AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0871800


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0871800 MtrunA17_Chr6g0453121 92.035 113 9 0 1 113 346 458 3.06e-65 204
Msa0871800 MtrunA17_Chr6g0450671 36.607 112 70 1 1 112 319 429 9.39e-17 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0871800 AT5G39830.2 67.257 113 37 0 1 113 322 434 7.54e-47 155
Msa0871800 AT5G39830.1 67.257 113 37 0 1 113 336 448 9.53e-47 155
Msa0871800 AT3G27925.1 36.937 111 69 1 1 111 326 435 2.32e-15 70.9
Msa0871800 AT3G27925.2 36.937 111 69 1 1 111 326 435 2.32e-15 70.9

Find 29 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCAATGATTCGTCAATTTA+AGG 0.147774 6_1:+6982843 Msa0871800:exon
AGGAGAGTTGTTGAAGGTAT+TGG 0.326012 6_1:+6983645 Msa0871800:CDS
GAATCATTGACTTAAGTATA+AGG 0.337537 6_1:-6982831 None:intergenic
AGTTGATTTGCAATCAAGTC+TGG 0.378170 6_1:-6982898 None:intergenic
ATATTACTGCATACAGTACT+TGG 0.391357 6_1:-6982775 None:intergenic
CCCACTGCAAGAGGATTTGC+TGG 0.394473 6_1:+6983089 Msa0871800:CDS
CAATTTAAGGTTGTTAGAGC+TGG 0.421502 6_1:+6982856 Msa0871800:CDS
CACAAGCAAACTTGTAATGT+TGG 0.437303 6_1:+6982741 Msa0871800:five_prime_UTR
AATCAACTTAACGTTCGAAA+TGG 0.442751 6_1:+6982913 Msa0871800:CDS
TTTGCTGGTAATATAGTCCT+TGG 0.459672 6_1:+6983104 Msa0871800:CDS
AATAGTCTTGCTGCCAAAGT+AGG 0.466843 6_1:+6983059 Msa0871800:CDS
TCAAAGAGGTGATGAAAAGT+TGG 0.474785 6_1:+6983705 Msa0871800:CDS
ACAAGCAAACTTGTAATGTT+GGG 0.490913 6_1:+6982742 Msa0871800:five_prime_UTR
AATCATTGACTTAAGTATAA+GGG 0.494987 6_1:-6982830 None:intergenic
AGTGGGATTTAGTCCTACTT+TGG 0.499080 6_1:-6983072 None:intergenic
CTCTGTTCCTCAAGTACCAT+AGG 0.543147 6_1:-6983733 None:intergenic
AAATGGAGCTCTTATTCGTA+CGG 0.547905 6_1:+6982930 Msa0871800:CDS
GAGCAAAGGAGAGTTGTTGA+AGG 0.562502 6_1:+6983639 Msa0871800:CDS
TGAAAAGTTGGAGCTGCCTA+TGG 0.567740 6_1:+6983717 Msa0871800:CDS
GTATTGGATGAGTATAATGT+AGG 0.571729 6_1:+6983661 Msa0871800:CDS
CCAGCAAATCCTCTTGCAGT+GGG 0.578110 6_1:-6983089 None:intergenic
TGTTAGAGCTGGATTGAATG+TGG 0.584555 6_1:+6982867 Msa0871800:CDS
GTAATGTTGTTGATTCAAAG+AGG 0.584814 6_1:+6983691 Msa0871800:CDS
TATTACTGCATACAGTACTT+GGG 0.591003 6_1:-6982774 None:intergenic
GGACTAAATCCCACTGCAAG+AGG 0.592687 6_1:+6983080 Msa0871800:CDS
ACCAGCAAATCCTCTTGCAG+TGG 0.597269 6_1:-6983090 None:intergenic
GGAGCTGCCTATGGTACTTG+AGG 0.600833 6_1:+6983726 Msa0871800:CDS
CTGCAATGATGATATCTCCA+AGG 0.619534 6_1:-6983121 None:intergenic
TCTTGGTAGGTCAAGAGCAA+AGG 0.624326 6_1:+6983625 Msa0871800:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTAATTTGAAAACTT+TGG + chr6_1:6983597-6983616 Msa0871800:intron 10.0%
!!! TTTAATTAATTTGAAAACTT+TGG + chr6_1:6983597-6983616 Msa0871800:intron 10.0%
!!! TTATTTTATTTCGTAACAAA+CGG + chr6_1:6983452-6983471 Msa0871800:intron 15.0%
!!! GTAATAAGAAATGAATATTT+TGG - chr6_1:6983496-6983515 None:intergenic 15.0%
!!! TTATTTTATTTCGTAACAAA+CGG + chr6_1:6983452-6983471 Msa0871800:intron 15.0%
!!! GTAATAAGAAATGAATATTT+TGG - chr6_1:6983496-6983515 None:intergenic 15.0%
!!! AATCATTGACTTAAGTATAA+GGG - chr6_1:6982833-6982852 None:intergenic 20.0%
!! ATAAATACAAAGTTTGATTG+TGG - chr6_1:6982982-6983001 None:intergenic 20.0%
!! TCTTTACAAAAATTGAGATT+TGG + chr6_1:6983239-6983258 Msa0871800:intron 20.0%
!!! TATTTTATTTCGTAACAAAC+GGG + chr6_1:6983453-6983472 Msa0871800:intron 20.0%
!!! TAGAGTTTTACATAAAACAA+TGG - chr6_1:6983555-6983574 None:intergenic 20.0%
!!! TAAGTTCAGTTTTATTTTAG+TGG + chr6_1:6983871-6983890 Msa0871800:three_prime_UTR 20.0%
!!! AAGTTCAGTTTTATTTTAGT+GGG + chr6_1:6983872-6983891 Msa0871800:three_prime_UTR 20.0%
!!! AATCATTGACTTAAGTATAA+GGG - chr6_1:6982833-6982852 None:intergenic 20.0%
!! ATAAATACAAAGTTTGATTG+TGG - chr6_1:6982982-6983001 None:intergenic 20.0%
!! TCTTTACAAAAATTGAGATT+TGG + chr6_1:6983239-6983258 Msa0871800:intron 20.0%
!!! TATTTTATTTCGTAACAAAC+GGG + chr6_1:6983453-6983472 Msa0871800:intron 20.0%
!!! TAGAGTTTTACATAAAACAA+TGG - chr6_1:6983555-6983574 None:intergenic 20.0%
!!! TAAGTTCAGTTTTATTTTAG+TGG + chr6_1:6983871-6983890 Msa0871800:three_prime_UTR 20.0%
!!! AAGTTCAGTTTTATTTTAGT+GGG + chr6_1:6983872-6983891 Msa0871800:three_prime_UTR 20.0%
!!! GAATCATTGACTTAAGTATA+AGG - chr6_1:6982834-6982853 None:intergenic 25.0%
!!! GTTTCAAAAAAACCACTTTT+AGG + chr6_1:6983192-6983211 Msa0871800:intron 25.0%
! GAAAAATGTGATTGTCATTA+GGG + chr6_1:6983340-6983359 Msa0871800:intron 25.0%
! ATGTAAAACTCTACCTTTAT+AGG + chr6_1:6983562-6983581 Msa0871800:intron 25.0%
!!! GAATCATTGACTTAAGTATA+AGG - chr6_1:6982834-6982853 None:intergenic 25.0%
!!! GTTTCAAAAAAACCACTTTT+AGG + chr6_1:6983192-6983211 Msa0871800:intron 25.0%
! GAAAAATGTGATTGTCATTA+GGG + chr6_1:6983340-6983359 Msa0871800:intron 25.0%
! ATGTAAAACTCTACCTTTAT+AGG + chr6_1:6983562-6983581 Msa0871800:intron 25.0%
ACAAGCAAACTTGTAATGTT+GGG + chr6_1:6982742-6982761 Msa0871800:five_prime_UTR 30.0%
TATTACTGCATACAGTACTT+GGG - chr6_1:6982777-6982796 None:intergenic 30.0%
ATATTACTGCATACAGTACT+TGG - chr6_1:6982778-6982797 None:intergenic 30.0%
GTCAATGATTCGTCAATTTA+AGG + chr6_1:6982843-6982862 Msa0871800:exon 30.0%
AATCAACTTAACGTTCGAAA+TGG + chr6_1:6982913-6982932 Msa0871800:CDS 30.0%
GGTACAAGAAACAAATTTCA+TGG - chr6_1:6982961-6982980 None:intergenic 30.0%
!!! AGACTATTTTTTGGAACCTA+GGG - chr6_1:6983047-6983066 None:intergenic 30.0%
!!! AAGACTATTTTTTGGAACCT+AGG - chr6_1:6983048-6983067 None:intergenic 30.0%
TAAAGAGCTTCTGTAAATGA+AGG - chr6_1:6983166-6983185 None:intergenic 30.0%
CGGATAATAAATCCTAAAAG+TGG - chr6_1:6983207-6983226 None:intergenic 30.0%
TGTAAAGAATCTGTAACGAA+CGG - chr6_1:6983227-6983246 None:intergenic 30.0%
CGAAAAATGTGATTGTCATT+AGG + chr6_1:6983339-6983358 Msa0871800:intron 30.0%
AATCACTTGTCTACCTATAA+AGG - chr6_1:6983578-6983597 None:intergenic 30.0%
TGAAAACTTTGGTACATTCT+TGG + chr6_1:6983608-6983627 Msa0871800:intron 30.0%
GTATTGGATGAGTATAATGT+AGG + chr6_1:6983661-6983680 Msa0871800:CDS 30.0%
!! GTAATGTTGTTGATTCAAAG+AGG + chr6_1:6983691-6983710 Msa0871800:CDS 30.0%
ACAAGCAAACTTGTAATGTT+GGG + chr6_1:6982742-6982761 Msa0871800:five_prime_UTR 30.0%
TATTACTGCATACAGTACTT+GGG - chr6_1:6982777-6982796 None:intergenic 30.0%
ATATTACTGCATACAGTACT+TGG - chr6_1:6982778-6982797 None:intergenic 30.0%
GTCAATGATTCGTCAATTTA+AGG + chr6_1:6982843-6982862 Msa0871800:exon 30.0%
AATCAACTTAACGTTCGAAA+TGG + chr6_1:6982913-6982932 Msa0871800:CDS 30.0%
GGTACAAGAAACAAATTTCA+TGG - chr6_1:6982961-6982980 None:intergenic 30.0%
!!! AGACTATTTTTTGGAACCTA+GGG - chr6_1:6983047-6983066 None:intergenic 30.0%
!!! AAGACTATTTTTTGGAACCT+AGG - chr6_1:6983048-6983067 None:intergenic 30.0%
TAAAGAGCTTCTGTAAATGA+AGG - chr6_1:6983166-6983185 None:intergenic 30.0%
CGGATAATAAATCCTAAAAG+TGG - chr6_1:6983207-6983226 None:intergenic 30.0%
TGTAAAGAATCTGTAACGAA+CGG - chr6_1:6983227-6983246 None:intergenic 30.0%
CGAAAAATGTGATTGTCATT+AGG + chr6_1:6983339-6983358 Msa0871800:intron 30.0%
AATCACTTGTCTACCTATAA+AGG - chr6_1:6983578-6983597 None:intergenic 30.0%
TGAAAACTTTGGTACATTCT+TGG + chr6_1:6983608-6983627 Msa0871800:intron 30.0%
GTATTGGATGAGTATAATGT+AGG + chr6_1:6983661-6983680 Msa0871800:CDS 30.0%
!! GTAATGTTGTTGATTCAAAG+AGG + chr6_1:6983691-6983710 Msa0871800:CDS 30.0%
CACAAGCAAACTTGTAATGT+TGG + chr6_1:6982741-6982760 Msa0871800:five_prime_UTR 35.0%
! CAATTTAAGGTTGTTAGAGC+TGG + chr6_1:6982856-6982875 Msa0871800:CDS 35.0%
!! AGTTGATTTGCAATCAAGTC+TGG - chr6_1:6982901-6982920 None:intergenic 35.0%
AAATGGAGCTCTTATTCGTA+CGG + chr6_1:6982930-6982949 Msa0871800:CDS 35.0%
!!! TTGGCAGCAAGACTATTTTT+TGG - chr6_1:6983056-6983075 None:intergenic 35.0%
TTTGCTGGTAATATAGTCCT+TGG + chr6_1:6983104-6983123 Msa0871800:CDS 35.0%
! AACAATGGCATAAGAAGCAT+AGG - chr6_1:6983540-6983559 None:intergenic 35.0%
AACTTTGGTACATTCTTGGT+AGG + chr6_1:6983612-6983631 Msa0871800:intron 35.0%
TCAAAGAGGTGATGAAAAGT+TGG + chr6_1:6983705-6983724 Msa0871800:CDS 35.0%
CACAAGCAAACTTGTAATGT+TGG + chr6_1:6982741-6982760 Msa0871800:five_prime_UTR 35.0%
! CAATTTAAGGTTGTTAGAGC+TGG + chr6_1:6982856-6982875 Msa0871800:CDS 35.0%
!! AGTTGATTTGCAATCAAGTC+TGG - chr6_1:6982901-6982920 None:intergenic 35.0%
AAATGGAGCTCTTATTCGTA+CGG + chr6_1:6982930-6982949 Msa0871800:CDS 35.0%
!!! TTGGCAGCAAGACTATTTTT+TGG - chr6_1:6983056-6983075 None:intergenic 35.0%
TTTGCTGGTAATATAGTCCT+TGG + chr6_1:6983104-6983123 Msa0871800:CDS 35.0%
! AACAATGGCATAAGAAGCAT+AGG - chr6_1:6983540-6983559 None:intergenic 35.0%
AACTTTGGTACATTCTTGGT+AGG + chr6_1:6983612-6983631 Msa0871800:intron 35.0%
TCAAAGAGGTGATGAAAAGT+TGG + chr6_1:6983705-6983724 Msa0871800:CDS 35.0%
TGTTAGAGCTGGATTGAATG+TGG + chr6_1:6982867-6982886 Msa0871800:CDS 40.0%
CTCTAAACCTTATGTTCCCT+AGG + chr6_1:6983028-6983047 Msa0871800:intron 40.0%
! TTTGGAACCTAGGGAACATA+AGG - chr6_1:6983038-6983057 None:intergenic 40.0%
! AATAGTCTTGCTGCCAAAGT+AGG + chr6_1:6983059-6983078 Msa0871800:CDS 40.0%
AGTGGGATTTAGTCCTACTT+TGG - chr6_1:6983075-6983094 None:intergenic 40.0%
CTGCAATGATGATATCTCCA+AGG - chr6_1:6983124-6983143 None:intergenic 40.0%
TTCTGTAAATGAAGGCTCAC+AGG - chr6_1:6983158-6983177 None:intergenic 40.0%
! AGGAGAGTTGTTGAAGGTAT+TGG + chr6_1:6983645-6983664 Msa0871800:CDS 40.0%
TGTTAGAGCTGGATTGAATG+TGG + chr6_1:6982867-6982886 Msa0871800:CDS 40.0%
CTCTAAACCTTATGTTCCCT+AGG + chr6_1:6983028-6983047 Msa0871800:intron 40.0%
! TTTGGAACCTAGGGAACATA+AGG - chr6_1:6983038-6983057 None:intergenic 40.0%
! AATAGTCTTGCTGCCAAAGT+AGG + chr6_1:6983059-6983078 Msa0871800:CDS 40.0%
AGTGGGATTTAGTCCTACTT+TGG - chr6_1:6983075-6983094 None:intergenic 40.0%
CTGCAATGATGATATCTCCA+AGG - chr6_1:6983124-6983143 None:intergenic 40.0%
TTCTGTAAATGAAGGCTCAC+AGG - chr6_1:6983158-6983177 None:intergenic 40.0%
! AGGAGAGTTGTTGAAGGTAT+TGG + chr6_1:6983645-6983664 Msa0871800:CDS 40.0%
TCTTGGTAGGTCAAGAGCAA+AGG + chr6_1:6983625-6983644 Msa0871800:intron 45.0%
GAGCAAAGGAGAGTTGTTGA+AGG + chr6_1:6983639-6983658 Msa0871800:CDS 45.0%
TGAAAAGTTGGAGCTGCCTA+TGG + chr6_1:6983717-6983736 Msa0871800:CDS 45.0%
CTCTGTTCCTCAAGTACCAT+AGG - chr6_1:6983736-6983755 None:intergenic 45.0%
TCTTGGTAGGTCAAGAGCAA+AGG + chr6_1:6983625-6983644 Msa0871800:intron 45.0%
GAGCAAAGGAGAGTTGTTGA+AGG + chr6_1:6983639-6983658 Msa0871800:CDS 45.0%
TGAAAAGTTGGAGCTGCCTA+TGG + chr6_1:6983717-6983736 Msa0871800:CDS 45.0%
CTCTGTTCCTCAAGTACCAT+AGG - chr6_1:6983736-6983755 None:intergenic 45.0%
GGACTAAATCCCACTGCAAG+AGG + chr6_1:6983080-6983099 Msa0871800:CDS 50.0%
CCAGCAAATCCTCTTGCAGT+GGG - chr6_1:6983092-6983111 None:intergenic 50.0%
ACCAGCAAATCCTCTTGCAG+TGG - chr6_1:6983093-6983112 None:intergenic 50.0%
GGACTAAATCCCACTGCAAG+AGG + chr6_1:6983080-6983099 Msa0871800:CDS 50.0%
CCAGCAAATCCTCTTGCAGT+GGG - chr6_1:6983092-6983111 None:intergenic 50.0%
ACCAGCAAATCCTCTTGCAG+TGG - chr6_1:6983093-6983112 None:intergenic 50.0%
CCCACTGCAAGAGGATTTGC+TGG + chr6_1:6983089-6983108 Msa0871800:CDS 55.0%
GGAGCTGCCTATGGTACTTG+AGG + chr6_1:6983726-6983745 Msa0871800:CDS 55.0%
CCCACTGCAAGAGGATTTGC+TGG + chr6_1:6983089-6983108 Msa0871800:CDS 55.0%
GGAGCTGCCTATGGTACTTG+AGG + chr6_1:6983726-6983745 Msa0871800:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 6982733 6983899 6982733 ID=Msa0871800;Name=Msa0871800
chr6_1 mRNA 6982733 6983899 6982733 ID=Msa0871800-mRNA-1;Parent=Msa0871800;Name=Msa0871800-mRNA-1;_AED=0.00;_eAED=0.00;_QI=114|1|1|1|1|1|3|137|113
chr6_1 exon 6982733 6982951 6982733 ID=Msa0871800-mRNA-1:exon:2116;Parent=Msa0871800-mRNA-1
chr6_1 exon 6983050 6983157 6983050 ID=Msa0871800-mRNA-1:exon:2117;Parent=Msa0871800-mRNA-1
chr6_1 exon 6983634 6983899 6983634 ID=Msa0871800-mRNA-1:exon:2118;Parent=Msa0871800-mRNA-1
chr6_1 five_prime_UTR 6982733 6982846 6982733 ID=Msa0871800-mRNA-1:five_prime_utr;Parent=Msa0871800-mRNA-1
chr6_1 CDS 6982847 6982951 6982847 ID=Msa0871800-mRNA-1:cds;Parent=Msa0871800-mRNA-1
chr6_1 CDS 6983050 6983157 6983050 ID=Msa0871800-mRNA-1:cds;Parent=Msa0871800-mRNA-1
chr6_1 CDS 6983634 6983762 6983634 ID=Msa0871800-mRNA-1:cds;Parent=Msa0871800-mRNA-1
chr6_1 three_prime_UTR 6983763 6983899 6983763 ID=Msa0871800-mRNA-1:three_prime_utr;Parent=Msa0871800-mRNA-1
Gene Sequence

>Msa0871800

ATGATTCGTCAATTTAAGGTTGTTAGAGCTGGATTGAATGTGGATATTGCTCCAGACTTGATTGCAAATCAACTTAACGTTCGAAATGGAGCTCTTATTCGTACGGTTCCAAAAAATAGTCTTGCTGCCAAAGTAGGACTAAATCCCACTGCAAGAGGATTTGCTGGTAATATAGTCCTTGGAGATATCATCATTGCAGTTGACAATAAACCTGTCAAGAGCAAAGGAGAGTTGTTGAAGGTATTGGATGAGTATAATGTAGGAGAAAAAGTAATGTTGTTGATTCAAAGAGGTGATGAAAAGTTGGAGCTGCCTATGGTACTTGAGGAACAGAGTTCATGA

Protein sequence

>Msa0871800

MIRQFKVVRAGLNVDIAPDLIANQLNVRNGALIRTVPKNSLAAKVGLNPTARGFAGNIVLGDIIIAVDNKPVKSKGELLKVLDEYNVGEKVMLLIQRGDEKLELPMVLEEQSS*