AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1210400


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1210400 MtrunA17_Chr4g0059621 95.833 48 2 0 1 48 1 48 8.84e-28 94.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGTGTTGTGAATCGAAAA+TGG 0.327426 8_1:+69310566 Msa1210400:exon
ATGGGAATAGCTGGTAAGAT+TGG 0.362277 8_1:+69310585 Msa1210400:CDS
TGGGAATAGCTGGTAAGATT+GGG 0.374359 8_1:+69310586 Msa1210400:CDS
GATTGGGGATTACACTTTCA+AGG 0.398797 8_1:+69310602 Msa1210400:CDS
AAGGCCTTTGTAAACATTAA+CGG 0.435862 8_1:-69310676 None:intergenic
TCCAATTAGGGGCTAGAGAA+AGG 0.438832 8_1:+69311244 Msa1210400:intron
TTGTGTTGTGAATCGAAAAT+GGG 0.473456 8_1:+69310567 Msa1210400:exon
GAATCGAAAATGGGAATAGC+TGG 0.500649 8_1:+69310576 Msa1210400:exon
CGACAGAGTATAACTTCGTA+AGG 0.505152 8_1:+69310515 None:intergenic
ATGTTTACAAAGGCCTTGCT+TGG 0.507155 8_1:+69310682 Msa1210400:CDS
TTCGATTCACAACACAAACT+AGG 0.520675 8_1:-69310561 None:intergenic
AAGGCTTTCACAGCAACTCT+CGG 0.525587 8_1:+69310621 Msa1210400:CDS
GGGAATAGCTGGTAAGATTG+GGG 0.543838 8_1:+69310587 Msa1210400:CDS
AACATTAACGGAGAAAGTGC+CGG 0.561590 8_1:-69310664 None:intergenic
TTCTCCGTTAATGTTTACAA+AGG 0.561828 8_1:+69310672 Msa1210400:CDS
TTCGTAAGGGTGTGTGAGCG+TGG 0.563753 8_1:+69310529 Msa1210400:five_prime_UTR
ATTGAGCTTTGTGCCAAGCA+AGG 0.577529 8_1:-69310695 None:intergenic
ATCACTACTATCTACCTCAC+CGG 0.580585 8_1:+69310645 Msa1210400:CDS
TAACGGAGAAAGTGCCGGTG+AGG 0.652823 8_1:-69310659 None:intergenic
GACAGAGTATAACTTCGTAA+GGG 0.688135 8_1:+69310516 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTACTAAATATGAATTAAA+TGG - chr8_1:69310852-69310871 None:intergenic 15.0%
!! AGTACTAAATATGAATTAAA+TGG - chr8_1:69310852-69310871 None:intergenic 15.0%
!! GTATAAAATATGAATGATCA+AGG - chr8_1:69310963-69310982 None:intergenic 20.0%
!!! AATTTGTTGTTTATCCAATT+AGG + chr8_1:69311231-69311250 Msa1210400:intron 20.0%
!!! ATTTGTTGTTTATCCAATTA+GGG + chr8_1:69311232-69311251 Msa1210400:intron 20.0%
!! GTATAAAATATGAATGATCA+AGG - chr8_1:69310963-69310982 None:intergenic 20.0%
!!! AATTTGTTGTTTATCCAATT+AGG + chr8_1:69311231-69311250 Msa1210400:intron 20.0%
!!! ATTTGTTGTTTATCCAATTA+GGG + chr8_1:69311232-69311251 Msa1210400:intron 20.0%
! GAAGATTGAAACAAAGTAAT+TGG - chr8_1:69310823-69310842 None:intergenic 25.0%
!! ATGTCATAAACTGATTTCAT+AGG - chr8_1:69311039-69311058 None:intergenic 25.0%
!!! TTTGTTGTTTATCCAATTAG+GGG + chr8_1:69311233-69311252 Msa1210400:intron 25.0%
! GAAGATTGAAACAAAGTAAT+TGG - chr8_1:69310823-69310842 None:intergenic 25.0%
!! ATGTCATAAACTGATTTCAT+AGG - chr8_1:69311039-69311058 None:intergenic 25.0%
!!! TTTGTTGTTTATCCAATTAG+GGG + chr8_1:69311233-69311252 Msa1210400:intron 25.0%
TTTGTGTTGTGAATCGAAAA+TGG + chr8_1:69310566-69310585 Msa1210400:exon 30.0%
TTGTGTTGTGAATCGAAAAT+GGG + chr8_1:69310567-69310586 Msa1210400:exon 30.0%
TTCTCCGTTAATGTTTACAA+AGG + chr8_1:69310672-69310691 Msa1210400:CDS 30.0%
AAGGCCTTTGTAAACATTAA+CGG - chr8_1:69310679-69310698 None:intergenic 30.0%
! TGTAGAATTGTTACTTGATC+TGG + chr8_1:69310919-69310938 Msa1210400:intron 30.0%
! TCTCTTTTCATAAGCTATCT+TGG - chr8_1:69311099-69311118 None:intergenic 30.0%
TTTGTGTTGTGAATCGAAAA+TGG + chr8_1:69310566-69310585 Msa1210400:exon 30.0%
TTGTGTTGTGAATCGAAAAT+GGG + chr8_1:69310567-69310586 Msa1210400:exon 30.0%
TTCTCCGTTAATGTTTACAA+AGG + chr8_1:69310672-69310691 Msa1210400:CDS 30.0%
AAGGCCTTTGTAAACATTAA+CGG - chr8_1:69310679-69310698 None:intergenic 30.0%
! TGTAGAATTGTTACTTGATC+TGG + chr8_1:69310919-69310938 Msa1210400:intron 30.0%
! TCTCTTTTCATAAGCTATCT+TGG - chr8_1:69311099-69311118 None:intergenic 30.0%
TTCGATTCACAACACAAACT+AGG - chr8_1:69310564-69310583 None:intergenic 35.0%
AGCTTATTTGTGAGACTGTT+TGG + chr8_1:69311008-69311027 Msa1210400:intron 35.0%
GCTTATTTGTGAGACTGTTT+GGG + chr8_1:69311009-69311028 Msa1210400:intron 35.0%
CTTATTTGTGAGACTGTTTG+GGG + chr8_1:69311010-69311029 Msa1210400:intron 35.0%
TTCGATTCACAACACAAACT+AGG - chr8_1:69310564-69310583 None:intergenic 35.0%
AGCTTATTTGTGAGACTGTT+TGG + chr8_1:69311008-69311027 Msa1210400:intron 35.0%
GCTTATTTGTGAGACTGTTT+GGG + chr8_1:69311009-69311028 Msa1210400:intron 35.0%
CTTATTTGTGAGACTGTTTG+GGG + chr8_1:69311010-69311029 Msa1210400:intron 35.0%
GAATCGAAAATGGGAATAGC+TGG + chr8_1:69310576-69310595 Msa1210400:exon 40.0%
ATGGGAATAGCTGGTAAGAT+TGG + chr8_1:69310585-69310604 Msa1210400:CDS 40.0%
TGGGAATAGCTGGTAAGATT+GGG + chr8_1:69310586-69310605 Msa1210400:CDS 40.0%
GATTGGGGATTACACTTTCA+AGG + chr8_1:69310602-69310621 Msa1210400:CDS 40.0%
ATCACTACTATCTACCTCAC+CGG + chr8_1:69310645-69310664 Msa1210400:CDS 40.0%
! AACATTAACGGAGAAAGTGC+CGG - chr8_1:69310667-69310686 None:intergenic 40.0%
ATGTTTACAAAGGCCTTGCT+TGG + chr8_1:69310682-69310701 Msa1210400:CDS 40.0%
AAGCTATCTTGGAGAGCTTA+CGG - chr8_1:69311088-69311107 None:intergenic 40.0%
GAATCGAAAATGGGAATAGC+TGG + chr8_1:69310576-69310595 Msa1210400:exon 40.0%
ATGGGAATAGCTGGTAAGAT+TGG + chr8_1:69310585-69310604 Msa1210400:CDS 40.0%
TGGGAATAGCTGGTAAGATT+GGG + chr8_1:69310586-69310605 Msa1210400:CDS 40.0%
GATTGGGGATTACACTTTCA+AGG + chr8_1:69310602-69310621 Msa1210400:CDS 40.0%
ATCACTACTATCTACCTCAC+CGG + chr8_1:69310645-69310664 Msa1210400:CDS 40.0%
! AACATTAACGGAGAAAGTGC+CGG - chr8_1:69310667-69310686 None:intergenic 40.0%
ATGTTTACAAAGGCCTTGCT+TGG + chr8_1:69310682-69310701 Msa1210400:CDS 40.0%
AAGCTATCTTGGAGAGCTTA+CGG - chr8_1:69311088-69311107 None:intergenic 40.0%
GGGAATAGCTGGTAAGATTG+GGG + chr8_1:69310587-69310606 Msa1210400:CDS 45.0%
AAGGCTTTCACAGCAACTCT+CGG + chr8_1:69310621-69310640 Msa1210400:CDS 45.0%
! ATTGAGCTTTGTGCCAAGCA+AGG - chr8_1:69310698-69310717 None:intergenic 45.0%
GAGAGAGAGAAAGAGGGTTA+CGG - chr8_1:69310721-69310740 None:intergenic 45.0%
GAGAGAGAGAGAGAGAAAGA+GGG - chr8_1:69310727-69310746 None:intergenic 45.0%
AGAGAGAGAGAGAGAGAAAG+AGG - chr8_1:69310728-69310747 None:intergenic 45.0%
TCCAATTAGGGGCTAGAGAA+AGG + chr8_1:69311244-69311263 Msa1210400:intron 45.0%
GGGAATAGCTGGTAAGATTG+GGG + chr8_1:69310587-69310606 Msa1210400:CDS 45.0%
AAGGCTTTCACAGCAACTCT+CGG + chr8_1:69310621-69310640 Msa1210400:CDS 45.0%
! ATTGAGCTTTGTGCCAAGCA+AGG - chr8_1:69310698-69310717 None:intergenic 45.0%
GAGAGAGAGAAAGAGGGTTA+CGG - chr8_1:69310721-69310740 None:intergenic 45.0%
GAGAGAGAGAGAGAGAAAGA+GGG - chr8_1:69310727-69310746 None:intergenic 45.0%
AGAGAGAGAGAGAGAGAAAG+AGG - chr8_1:69310728-69310747 None:intergenic 45.0%
TCCAATTAGGGGCTAGAGAA+AGG + chr8_1:69311244-69311263 Msa1210400:intron 45.0%
GCCTTTCTCTAGCCCCTAAT+TGG - chr8_1:69311248-69311267 None:intergenic 50.0%
GCCTTTCTCTAGCCCCTAAT+TGG - chr8_1:69311248-69311267 None:intergenic 50.0%
! TTCGTAAGGGTGTGTGAGCG+TGG + chr8_1:69310529-69310548 Msa1210400:five_prime_UTR 55.0%
!! TAACGGAGAAAGTGCCGGTG+AGG - chr8_1:69310662-69310681 None:intergenic 55.0%
! TTCGTAAGGGTGTGTGAGCG+TGG + chr8_1:69310529-69310548 Msa1210400:five_prime_UTR 55.0%
!! TAACGGAGAAAGTGCCGGTG+AGG - chr8_1:69310662-69310681 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 69310529 69311288 69310529 ID=Msa1210400;Name=Msa1210400
chr8_1 mRNA 69310529 69311288 69310529 ID=Msa1210400-mRNA-1;Parent=Msa1210400;Name=Msa1210400-mRNA-1;_AED=0.43;_eAED=0.43;_QI=56|0|0.5|0.5|1|1|2|0|56
chr8_1 exon 69310529 69310719 69310529 ID=Msa1210400-mRNA-1:exon:23203;Parent=Msa1210400-mRNA-1
chr8_1 exon 69311253 69311288 69311253 ID=Msa1210400-mRNA-1:exon:23204;Parent=Msa1210400-mRNA-1
chr8_1 five_prime_UTR 69310529 69310584 69310529 ID=Msa1210400-mRNA-1:five_prime_utr;Parent=Msa1210400-mRNA-1
chr8_1 CDS 69310585 69310719 69310585 ID=Msa1210400-mRNA-1:cds;Parent=Msa1210400-mRNA-1
chr8_1 CDS 69311253 69311288 69311253 ID=Msa1210400-mRNA-1:cds;Parent=Msa1210400-mRNA-1
Gene Sequence

>Msa1210400

ATGGGAATAGCTGGTAAGATTGGGGATTACACTTTCAAGGCTTTCACAGCAACTCTCGGCATCACTACTATCTACCTCACCGGCACTTTCTCCGTTAATGTTTACAAAGGCCTTGCTTGGCACAAAGCTCAATCCGGGCTAGAGAAAGGCATTTTTGTTGTTAATCAATAA

Protein sequence

>Msa1210400

MGIAGKIGDYTFKAFTATLGITTIYLTGTFSVNVYKGLAWHKAQSGLEKGIFVVNQ*