AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene008570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008570.t1 MTR_3g465990 92.842 461 33 0 1 461 1 461 0.0 894
MS.gene008570.t1 MTR_3g465980 91.323 461 40 0 1 461 1 461 0.0 877
MS.gene008570.t1 MTR_3g465930 68.240 466 140 5 1 460 1 464 0.0 667
MS.gene008570.t1 MTR_3g465910 68.182 462 138 5 1 457 1 458 0.0 662
MS.gene008570.t1 MTR_3g465880 67.021 470 144 6 1 461 1 468 0.0 641
MS.gene008570.t1 MTR_3g465950 67.634 448 133 3 1 445 1 439 0.0 619
MS.gene008570.t1 MTR_3g465870 61.720 465 133 7 1 460 1 425 0.0 560
MS.gene008570.t1 MTR_6g053810 56.774 465 183 10 4 457 5 462 4.96e-179 509
MS.gene008570.t1 MTR_4g055170 50.959 469 202 12 4 460 6 458 8.74e-155 447
MS.gene008570.t1 MTR_2g015430 32.237 456 277 15 10 455 2 435 5.38e-65 216
MS.gene008570.t1 MTR_8g096530 28.541 466 272 18 8 455 5 427 2.98e-42 155
MS.gene008570.t1 MTR_7g027175 30.730 397 236 17 17 401 23 392 2.71e-40 150
MS.gene008570.t1 MTR_7g110610 31.977 344 174 13 17 355 12 300 6.44e-40 148
MS.gene008570.t1 MTR_1g040215 30.811 370 223 11 4 361 3 351 1.14e-39 149
MS.gene008570.t1 MTR_3g098980 28.132 455 281 19 8 452 12 430 9.42e-39 145
MS.gene008570.t1 MTR_1g040175 30.227 397 240 13 17 401 16 387 2.52e-38 145
MS.gene008570.t1 MTR_3g071320 27.803 446 271 17 10 427 5 427 8.77e-38 143
MS.gene008570.t1 MTR_1g081660 28.070 456 280 15 17 457 25 447 2.89e-36 139
MS.gene008570.t1 MTR_5g076700 27.296 392 260 10 17 401 17 390 6.48e-36 138
MS.gene008570.t1 MTR_7g114520 29.620 395 248 13 17 401 18 392 4.32e-35 136
MS.gene008570.t1 MTR_1g053315 28.294 463 275 22 18 454 26 457 3.60e-34 134
MS.gene008570.t1 MTR_3g112180 24.519 416 259 11 18 412 20 401 8.40e-34 132
MS.gene008570.t1 MTR_7g114470 28.426 394 254 12 17 401 18 392 1.96e-33 131
MS.gene008570.t1 MTR_8g075610 29.526 359 224 13 17 366 1 339 1.32e-32 128
MS.gene008570.t1 MTR_7g114550 28.645 391 255 10 17 401 17 389 1.42e-32 129
MS.gene008570.t1 MTR_1g040315 28.530 347 211 10 4 338 3 324 6.67e-32 126
MS.gene008570.t1 MTR_4g007540 27.696 408 248 18 10 396 3 384 1.45e-31 125
MS.gene008570.t1 MTR_1g040320 28.085 470 253 19 4 457 3 403 2.44e-31 125
MS.gene008570.t1 MTR_2g105330 25.280 447 274 17 10 424 3 421 2.46e-31 125
MS.gene008570.t1 MTR_2g064495 24.667 450 273 16 10 424 3 421 4.48e-31 124
MS.gene008570.t1 MTR_7g114560 29.462 353 225 9 17 361 17 353 6.39e-31 124
MS.gene008570.t1 MTR_7g114490 28.499 393 253 12 17 401 17 389 1.56e-29 121
MS.gene008570.t1 MTR_3g026950 25.791 411 250 16 20 396 12 401 4.30e-29 119
MS.gene008570.t1 MTR_1g040195 25.551 454 270 15 17 457 16 414 3.23e-27 113
MS.gene008570.t1 MTR_7g086000 27.381 420 263 19 18 409 12 417 3.36e-25 108
MS.gene008570.t1 MTR_7g084940 27.027 407 249 17 8 396 14 390 5.86e-24 104
MS.gene008570.t1 MTR_0502s0010 32.020 203 126 5 147 345 44 238 2.47e-23 98.6
MS.gene008570.t1 MTR_3g049150 26.289 388 238 16 12 376 4 366 3.10e-23 102
MS.gene008570.t1 MTR_7g085930 26.368 402 239 19 44 406 38 421 3.24e-23 102
MS.gene008570.t1 MTR_4g097090 30.272 294 170 13 68 343 65 341 5.95e-23 101
MS.gene008570.t1 MTR_4g127650 28.571 287 183 9 68 343 66 341 9.97e-22 97.8
MS.gene008570.t1 MTR_4g013050 25.641 351 200 11 18 359 12 310 1.11e-21 97.1
MS.gene008570.t1 MTR_7g085990 25.722 381 229 18 56 397 45 410 1.54e-19 91.3
MS.gene008570.t1 MTR_7g114510 27.358 318 196 12 151 457 8 301 2.34e-19 89.0
MS.gene008570.t1 MTR_7g084910 28.863 343 204 14 8 332 15 335 2.17e-18 87.4
MS.gene008570.t1 MTR_3g101930 30.000 280 164 11 68 329 70 335 7.40e-18 86.3
MS.gene008570.t1 MTR_0042s0190 28.326 233 157 6 169 401 1 223 1.33e-17 83.6
MS.gene008570.t1 MTR_0190s0020 26.296 270 181 7 68 329 63 322 1.58e-17 85.1
MS.gene008570.t1 MTR_3g101900 26.871 294 161 12 68 334 66 332 4.87e-17 83.6
MS.gene008570.t1 MTR_4g022760 28.114 281 174 10 68 331 55 324 7.38e-17 82.8
MS.gene008570.t1 MTR_3g101960 27.018 285 172 11 68 334 68 334 1.02e-16 82.8
MS.gene008570.t1 MTR_3g102020 26.882 279 180 10 68 334 69 335 1.53e-16 82.0
MS.gene008570.t1 MTR_4g022720 27.273 275 180 9 68 332 55 319 2.58e-16 81.3
MS.gene008570.t1 MTR_5g090880 25.436 401 256 14 54 426 55 440 2.82e-16 81.3
MS.gene008570.t1 MTR_4g022825 26.335 281 190 8 63 332 50 324 5.42e-16 80.1
MS.gene008570.t1 MTR_3g101970 25.806 279 169 10 68 334 69 321 1.78e-15 78.6
MS.gene008570.t1 MTR_7g056087 24.021 383 241 14 9 364 3 362 1.99e-15 78.6
MS.gene008570.t1 MTR_3g102010 25.436 287 178 12 68 334 65 335 1.51e-14 75.9
MS.gene008570.t1 MTR_4g022800 27.915 283 172 12 68 331 55 324 2.46e-14 75.1
MS.gene008570.t1 MTR_8g041770 25.092 271 181 8 68 329 64 321 1.18e-13 73.2
MS.gene008570.t1 MTR_7g114960 23.901 364 225 15 31 374 30 361 1.53e-13 72.8
MS.gene008570.t1 MTR_3g450710 24.204 314 213 11 68 364 65 370 1.74e-13 72.8
MS.gene008570.t1 MTR_3g088420 25.271 277 179 7 68 331 55 316 2.14e-13 72.4
MS.gene008570.t1 MTR_5g090810 25.321 312 210 10 68 364 66 369 1.50e-12 69.7
MS.gene008570.t1 MTR_3g086020 23.414 457 295 19 8 457 3 411 2.32e-12 68.9
MS.gene008570.t1 MTR_3g450690 23.794 311 215 8 68 364 72 374 3.51e-12 68.6
MS.gene008570.t1 MTR_5g090830 24.038 312 214 9 68 364 66 369 1.28e-11 67.0
MS.gene008570.t1 MTR_7g014200 22.642 371 238 12 69 404 70 426 1.62e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008570.t1 AT5G07080 38.544 467 240 15 8 457 12 448 2.60e-94 292
MS.gene008570.t1 AT2G25150 37.284 464 261 13 8 460 11 455 1.46e-88 278
MS.gene008570.t1 AT2G23510 37.694 451 251 15 17 457 20 450 2.57e-88 277
MS.gene008570.t1 AT3G47170 35.729 473 260 15 10 457 12 465 5.39e-82 261
MS.gene008570.t1 AT1G28680 35.011 457 267 13 10 455 4 441 1.50e-78 251
MS.gene008570.t1 AT1G28680 36.043 369 210 11 10 367 4 357 2.05e-66 218
MS.gene008570.t1 AT3G62160 29.574 399 240 13 16 405 11 377 3.65e-47 168
MS.gene008570.t1 AT5G41040 28.166 458 282 17 8 453 15 437 3.68e-47 169
MS.gene008570.t1 AT5G41040 28.758 459 278 18 8 453 31 453 4.33e-47 169
MS.gene008570.t1 AT3G48720 29.490 451 271 20 13 452 12 426 1.08e-42 156
MS.gene008570.t1 AT3G03480 29.500 400 248 12 7 396 16 391 3.91e-41 152
MS.gene008570.t1 AT5G17540 27.601 471 284 15 7 457 6 439 8.54e-39 146
MS.gene008570.t1 AT5G63560 29.551 423 248 18 13 421 12 398 5.01e-37 141
MS.gene008570.t1 AT2G40230 27.404 416 265 15 10 411 5 397 1.23e-33 131
MS.gene008570.t1 AT5G48930 27.073 410 252 16 8 396 1 384 2.64e-33 130
MS.gene008570.t1 AT1G27620 26.506 415 249 11 17 411 16 394 4.43e-32 127
MS.gene008570.t1 AT1G65450 26.609 466 289 18 8 455 16 446 8.58e-26 109
MS.gene008570.t1 AT1G03390 25.977 435 271 18 20 432 32 437 7.74e-25 107
MS.gene008570.t1 AT2G19070 29.268 410 249 18 67 454 57 447 1.52e-23 103
MS.gene008570.t1 AT4G31910 26.098 410 249 18 28 405 29 416 5.30e-20 92.8
MS.gene008570.t1 AT4G15390 23.909 481 296 19 4 459 2 437 3.81e-18 87.0
MS.gene008570.t1 AT4G15390 23.909 481 296 19 4 459 2 437 3.81e-18 87.0
MS.gene008570.t1 AT1G24430 25.183 409 244 19 69 461 63 425 1.37e-17 85.1
MS.gene008570.t1 AT5G07870 27.863 262 166 9 68 314 72 325 3.36e-16 81.3
MS.gene008570.t1 AT5G07860 27.099 262 160 11 68 315 72 316 1.94e-15 78.6
MS.gene008570.t1 AT5G47950 33.163 196 99 10 7 191 3 177 1.93e-14 75.5
MS.gene008570.t1 AT5G23940 27.692 260 170 11 68 315 65 318 1.64e-13 72.8
MS.gene008570.t1 AT3G30280 23.602 483 292 23 7 461 3 436 3.48e-13 71.6
MS.gene008570.t1 AT5G07850 25.000 316 202 13 68 365 70 368 3.24e-12 68.6
MS.gene008570.t1 AT5G23970 23.992 471 287 23 8 459 1 419 5.04e-11 65.1
MS.gene008570.t1 AT3G50280 22.346 358 247 13 68 409 59 401 6.68e-11 64.7

Find 94 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTACCTAATGGATATTA+TGG 0.105930 3.3:-54039765 MS.gene008570:CDS
CATCTTTCATTTCAAGCTTT+AGG 0.193928 3.3:+54041337 None:intergenic
CCTTTCTTCTGTTTGTTACT+TGG 0.204100 3.3:-54040330 MS.gene008570:CDS
TCATCATTGTCAAATTTATT+AGG 0.246582 3.3:+54039420 None:intergenic
TCCTTGTTCATAGGGCATTC+TGG 0.247654 3.3:+54040047 None:intergenic
ATCTTTCATTTCAAGCTTTA+GGG 0.261824 3.3:+54041338 None:intergenic
TTGACAGAGTGGAAACATTT+AGG 0.264666 3.3:-54039546 MS.gene008570:CDS
CTGTAGAGTTGTCTTGATTC+AGG 0.273504 3.3:+54040003 None:intergenic
CTTGATGGAGATGTAGATTT+TGG 0.274005 3.3:-54039519 MS.gene008570:CDS
AACAATTCATGATTCTAATT+CGG 0.274186 3.3:-54041199 MS.gene008570:CDS
AAATTATGGTGTTCCATTCT+TGG 0.284915 3.3:-54040372 MS.gene008570:CDS
ACAATTCATGATTCTAATTC+GGG 0.288860 3.3:-54041198 MS.gene008570:CDS
TGGGAAAGAGAGAGACTTAT+TGG 0.299884 3.3:-54040092 MS.gene008570:CDS
TTCAATTTCCTCCTTGAACT+TGG 0.303476 3.3:+54039341 None:intergenic
GCAACAGATTCCTTGTTCAT+AGG 0.312948 3.3:+54040038 None:intergenic
AATCCTAATGCAGAAAATTA+TGG 0.321826 3.3:-54040386 MS.gene008570:CDS
TTAACCCCTCCTTTCATTGA+TGG 0.333155 3.3:+54039396 None:intergenic
AACTTACCCTTTGGTGTTCA+AGG 0.353325 3.3:-54040255 MS.gene008570:CDS
TCCCCTTGCAAGTTCGATAA+TGG 0.359679 3.3:+54040142 None:intergenic
TTCATAGGGCATTCTGGAAA+TGG 0.363471 3.3:+54040053 None:intergenic
AACTTGGGCATGGCAGCAAC+AGG 0.371483 3.3:+54039357 None:intergenic
CACAGAAATTGCAAAGCATT+TGG 0.379681 3.3:-54040297 MS.gene008570:CDS
TGAAGGAAAGTGGAAATATA+AGG 0.398595 3.3:-54039917 MS.gene008570:CDS
CTTTACCTAATGGATATTAT+GGG 0.402882 3.3:-54039764 MS.gene008570:CDS
TTAGCAATTGCTTCCAAGAA+TGG 0.403315 3.3:+54040359 None:intergenic
GGAGGTTTCACAATCGGAAT+GGG 0.409540 3.3:-54040215 MS.gene008570:CDS
ATGCAACTTGTTTGCGTCCA+TGG 0.416476 3.3:-54039463 MS.gene008570:CDS
ACAACTTTCGAAGCGATTGC+AGG 0.428496 3.3:-54039891 MS.gene008570:CDS
TGCTGCCATGCCCAAGTTCA+AGG 0.433774 3.3:-54039352 MS.gene008570:CDS
TGAAGACAAAACTTACCCTT+TGG 0.442212 3.3:-54040264 MS.gene008570:CDS
TCAATTTCCTCCTTGAACTT+GGG 0.445520 3.3:+54039342 None:intergenic
GAATTCCCATAATATCCATT+AGG 0.447150 3.3:+54039759 None:intergenic
CAACAGATTCCTTGTTCATA+GGG 0.458713 3.3:+54040039 None:intergenic
CGTTGCGGAGGTTTCACAAT+CGG 0.459106 3.3:-54040221 MS.gene008570:CDS
GCTTAAGTTGAGTTTGATGA+AGG 0.461955 3.3:-54039934 MS.gene008570:CDS
TGGTATTAGACGCAACTTGA+AGG 0.474505 3.3:-54039796 MS.gene008570:CDS
CCAAGTAACAAACAGAAGAA+AGG 0.486102 3.3:+54040330 None:intergenic
TTGAGTTTGATGAAGGAAAG+TGG 0.489083 3.3:-54039927 MS.gene008570:CDS
TTGTCACCTTGAACACCAAA+GGG 0.494129 3.3:+54040249 None:intergenic
CGAATTATAACGAGGAATCA+AGG 0.497566 3.3:-54039596 MS.gene008570:CDS
CTCAAACTGTTCATGTATAC+CGG 0.499140 3.3:-54041227 MS.gene008570:CDS
CAAGTAACAAACAGAAGAAA+GGG 0.512291 3.3:+54040331 None:intergenic
ATTCAAAGAAGCACTTTCAA+AGG 0.519773 3.3:-54041157 MS.gene008570:CDS
AATCATGAATTGTTGATGAC+CGG 0.519842 3.3:+54041208 None:intergenic
ATGCTTTGCAATTTCTGTGT+CGG 0.522721 3.3:+54040301 None:intergenic
TCTACGTCTTGCAGGTAGAA+TGG 0.526428 3.3:-54040447 MS.gene008570:intron
CCATAATATCCATTAGGTAA+AGG 0.529830 3.3:+54039765 None:intergenic
CGGAGGTTTCACAATCGGAA+TGG 0.531324 3.3:-54040216 MS.gene008570:CDS
TTCCTCCTTGAACTTGGGCA+TGG 0.531730 3.3:+54039347 None:intergenic
GCAAACAAGTTGCATGGTGC+TGG 0.535676 3.3:+54039471 None:intergenic
TTTGTCACCTTGAACACCAA+AGG 0.540211 3.3:+54040248 None:intergenic
TCATTGTCAAATTTATTAGG+AGG 0.540817 3.3:+54039423 None:intergenic
ATGGTGAAACATGCTGATGA+TGG 0.543171 3.3:-54040428 MS.gene008570:CDS
AAGCCATTATCGAACTTGCA+AGG 0.551949 3.3:-54040145 MS.gene008570:CDS
GAATCAAGGATGCTAAGTAC+TGG 0.553010 3.3:-54039582 MS.gene008570:CDS
AGCCATTATCGAACTTGCAA+GGG 0.556732 3.3:-54040144 MS.gene008570:CDS
ATGATCCATCAATGAAAGGA+GGG 0.556848 3.3:-54039401 MS.gene008570:CDS
GAGTTGTCTTGATTCAGGTA+AGG 0.559999 3.3:+54040008 None:intergenic
GCTAAGTACTGGTGCAGTAA+CGG 0.563324 3.3:-54039571 MS.gene008570:CDS
GAAAGTGAAAGAATACAAGA+AGG 0.574882 3.3:+54041285 None:intergenic
AATGATGATCCATCAATGAA+AGG 0.575055 3.3:-54039405 MS.gene008570:CDS
AGGTTAATAATTTCATAGAG+TGG 0.575699 3.3:+54039687 None:intergenic
TCCAGAATGCCCTATGAACA+AGG 0.582940 3.3:-54040048 MS.gene008570:CDS
AAGCGATTGCAGGTTATGTG+TGG 0.585990 3.3:-54039881 MS.gene008570:CDS
GAGGTTTCACAATCGGAATG+GGG 0.593146 3.3:-54040214 MS.gene008570:CDS
TCCCACACAGGTATCGTCGA+GGG 0.595299 3.3:+54040110 None:intergenic
AAACCTAGTATACCTGCAAG+AGG 0.599532 3.3:+54041117 None:intergenic
GTGACAAAATTTCGTTGCGG+AGG 0.602288 3.3:-54040233 MS.gene008570:CDS
GTGTTGCACGCTATCTGTGA+CGG 0.602692 3.3:-54040191 MS.gene008570:CDS
GCGTCTAATACCAGCTACAA+TGG 0.604485 3.3:+54039806 None:intergenic
GGTAGCTGTCTGATAAGCCA+TGG 0.606005 3.3:+54041359 None:intergenic
GCCCTAAAACTTAACAACAA+TGG 0.606771 3.3:-54039843 MS.gene008570:CDS
AGGTTTCACAATCGGAATGG+GGG 0.619066 3.3:-54040213 MS.gene008570:CDS
ATACATGAACAGTTTGAGCA+AGG 0.620162 3.3:+54041232 None:intergenic
AACCCTCGACGATACCTGTG+TGG 0.622732 3.3:-54040112 MS.gene008570:CDS
TTCCCACACAGGTATCGTCG+AGG 0.623361 3.3:+54040109 None:intergenic
AAGTTAGTGAGCTCAATGAA+AGG 0.624470 3.3:-54039710 MS.gene008570:CDS
CACGCTATCTGTGACGGAGT+TGG 0.625478 3.3:-54040185 MS.gene008570:CDS
GATGATCCATCAATGAAAGG+AGG 0.631222 3.3:-54039402 MS.gene008570:CDS
GCCATTATCGAACTTGCAAG+GGG 0.631450 3.3:-54040143 MS.gene008570:CDS
GCTTCTTTGAATACATGACA+AGG 0.632259 3.3:+54041168 None:intergenic
TGGAGGGTGAAATGATAACA+AGG 0.634756 3.3:-54039956 MS.gene008570:CDS
AGTCTCTCTCTTTCCCACAC+AGG 0.635132 3.3:+54040098 None:intergenic
ATGGACGCAAACAAGTTGCA+TGG 0.637526 3.3:+54039465 None:intergenic
CGATTGCAGGTTATGTGTGG+AGG 0.638354 3.3:-54039878 MS.gene008570:CDS
ACAGTGCTGACCATTGTAGC+TGG 0.644789 3.3:-54039816 MS.gene008570:CDS
TGATCCATCAATGAAAGGAG+GGG 0.653576 3.3:-54039400 MS.gene008570:CDS
ACCCTCGACGATACCTGTGT+GGG 0.660118 3.3:-54040111 MS.gene008570:CDS
ACTTGGGCATGGCAGCAACA+GGG 0.669220 3.3:+54039358 None:intergenic
CTTCTTTGAATACATGACAA+GGG 0.672661 3.3:+54041169 None:intergenic
ATATTATGGGAATTCATGTG+TGG 0.696693 3.3:-54039751 MS.gene008570:CDS
TGCCATGCCCAAGTTCAAGG+AGG 0.747229 3.3:-54039349 MS.gene008570:CDS
AAGGTGACAAAATTTCGTTG+CGG 0.748989 3.3:-54040236 MS.gene008570:CDS
GTTGGAGACGAATTATAACG+AGG 0.787236 3.3:-54039604 MS.gene008570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAATTTTAATAAAAATTT+TGG - chr3.3:54039727-54039746 MS.gene008570:CDS 0.0%
!!! TAAATTTTAATAAAAATTTT+GGG - chr3.3:54039728-54039747 MS.gene008570:CDS 0.0%
!! TTATAAGAAAAAAAATGATT+AGG + chr3.3:54039711-54039730 None:intergenic 10.0%
!!! AAATTATGATTTTAGTTTAT+AGG + chr3.3:54039826-54039845 None:intergenic 10.0%
!!! ATAGTCTTTTTTTTTTTTTT+TGG - chr3.3:54040062-54040081 MS.gene008570:CDS 10.0%
!!! TGAAATTTTGAATATTTATA+AGG + chr3.3:54039797-54039816 None:intergenic 10.0%
!! AAACATTTGTATTTAATGAA+AGG + chr3.3:54039596-54039615 None:intergenic 15.0%
!! TCTACCAAAAAAAATAAATA+TGG - chr3.3:54039649-54039668 MS.gene008570:CDS 15.0%
!! TTTCATAAAATATTCAAAAG+TGG + chr3.3:54040150-54040169 None:intergenic 15.0%
!!! AAAAAAAGACTATCATATTT+TGG + chr3.3:54040055-54040074 None:intergenic 15.0%
!!! AAAAAAGACTATCATATTTT+GGG + chr3.3:54040054-54040073 None:intergenic 15.0%
!!! AAAATTTATGTTAGCATTTT+CGG - chr3.3:54039919-54039938 MS.gene008570:CDS 15.0%
!!! AAATATTGTTTTAATATGGA+GGG - chr3.3:54040705-54040724 MS.gene008570:intron 15.0%
!!! AAATGTGATTTTATTTTGTA+AGG - chr3.3:54039979-54039998 MS.gene008570:CDS 15.0%
!!! ATGTTGACATAAAATTTTAA+AGG + chr3.3:54039855-54039874 None:intergenic 15.0%
!!! GAAATATATTTTTTTAGATG+AGG - chr3.3:54039754-54039773 MS.gene008570:CDS 15.0%
!!! GAAGAAATATTGTTTTAATA+TGG - chr3.3:54040701-54040720 MS.gene008570:intron 15.0%
!!! TAATATAAACCTTTAAAAGA+GGG - chr3.3:54039679-54039698 MS.gene008570:CDS 15.0%
!!! TACTAATGGAAAATTTTAAA+AGG + chr3.3:54039885-54039904 None:intergenic 15.0%
!! AACAATTCATGATTCTAATT+CGG - chr3.3:54039478-54039497 MS.gene008570:CDS 20.0%
!! TATTAGAATGTCCATAAAAA+TGG - chr3.3:54040175-54040194 MS.gene008570:CDS 20.0%
!! TCATCATTGTCAAATTTATT+AGG + chr3.3:54041260-54041279 None:intergenic 20.0%
!!! AAACTAGTGTTGTTAAAATA+TGG - chr3.3:54039617-54039636 MS.gene008570:CDS 20.0%
!!! ATGCTAACATAAATTTTACA+AGG + chr3.3:54039916-54039935 None:intergenic 20.0%
!!! ATTTTCCTTTAGCATTTTTA+AGG + chr3.3:54039946-54039965 None:intergenic 20.0%
!!! CTAATATAAACCTTTAAAAG+AGG - chr3.3:54039678-54039697 MS.gene008570:CDS 20.0%
!!! CTTTTAAAGGTTTATATTAG+AGG + chr3.3:54039678-54039697 None:intergenic 20.0%
!!! GAAATATTGTTTTAATATGG+AGG - chr3.3:54040704-54040723 MS.gene008570:intron 20.0%
!!! GCATCCATATTTATTTTTTT+TGG + chr3.3:54039656-54039675 None:intergenic 20.0%
!!! TATTTTACGAAAAATTGCAA+TGG - chr3.3:54040123-54040142 MS.gene008570:CDS 20.0%
!!! TGCAATTTTTCGTAAAATAA+TGG + chr3.3:54040122-54040141 None:intergenic 20.0%
!!! TTATTTTTTTTGGTAGATAG+AGG + chr3.3:54039646-54039665 None:intergenic 20.0%
!!! TTCCATAAAATCCATTTTTA+TGG + chr3.3:54040189-54040208 None:intergenic 20.0%
! AATCCTAATGCAGAAAATTA+TGG - chr3.3:54040291-54040310 MS.gene008570:CDS 25.0%
! ACAAGGACTAAAAATACTAA+TGG + chr3.3:54039899-54039918 None:intergenic 25.0%
! ACAATTCATGATTCTAATTC+GGG - chr3.3:54039479-54039498 MS.gene008570:CDS 25.0%
! AGGTTAATAATTTCATAGAG+TGG + chr3.3:54040993-54041012 None:intergenic 25.0%
! ATCTTTCATTTCAAGCTTTA+GGG + chr3.3:54039342-54039361 None:intergenic 25.0%
! TCATTGTCAAATTTATTAGG+AGG + chr3.3:54041257-54041276 None:intergenic 25.0%
!! CTTTACCTAATGGATATTAT+GGG - chr3.3:54040913-54040932 MS.gene008570:intron 25.0%
!! GATGAGGTTAGATATATTTT+TGG - chr3.3:54039770-54039789 MS.gene008570:CDS 25.0%
!! TGAATTTTTGACAAAATCAG+TGG + chr3.3:54041045-54041064 None:intergenic 25.0%
!! TTTTATTATTATCCTCTTGC+AGG - chr3.3:54039548-54039567 MS.gene008570:CDS 25.0%
!!! AAACATTTAGGTTTTCTTGA+TGG - chr3.3:54041143-54041162 MS.gene008570:CDS 25.0%
!!! ACCATTGTTGTTAAGTTTTA+GGG + chr3.3:54040838-54040857 None:intergenic 25.0%
!!! GTCCATAAAAATGGATTTTA+TGG - chr3.3:54040184-54040203 MS.gene008570:CDS 25.0%
AAATTATGGTGTTCCATTCT+TGG - chr3.3:54040305-54040324 MS.gene008570:CDS 30.0%
AATGATGATCCATCAATGAA+AGG - chr3.3:54041272-54041291 MS.gene008570:CDS 30.0%
ATATTATGGGAATTCATGTG+TGG - chr3.3:54040926-54040945 MS.gene008570:intron 30.0%
CAAAAATTCAATCAACACGT+TGG - chr3.3:54041055-54041074 MS.gene008570:intron 30.0%
CAAGTAACAAACAGAAGAAA+GGG + chr3.3:54040349-54040368 None:intergenic 30.0%
CATCTTTCATTTCAAGCTTT+AGG + chr3.3:54039343-54039362 None:intergenic 30.0%
CCATAATATCCATTAGGTAA+AGG + chr3.3:54040915-54040934 None:intergenic 30.0%
CTTCTTTGAATACATGACAA+GGG + chr3.3:54039511-54039530 None:intergenic 30.0%
GAAAGTGAAAGAATACAAGA+AGG + chr3.3:54039395-54039414 None:intergenic 30.0%
GAATTCCCATAATATCCATT+AGG + chr3.3:54040921-54040940 None:intergenic 30.0%
TGAAGGAAAGTGGAAATATA+AGG - chr3.3:54040760-54040779 MS.gene008570:intron 30.0%
TGTAAAAATGCTCATTTCCA+TGG + chr3.3:54041234-54041253 None:intergenic 30.0%
! ACACCATAATTTTCTGCATT+AGG + chr3.3:54040297-54040316 None:intergenic 30.0%
! ATTTTTGACAAAATCAGTGG+TGG + chr3.3:54041042-54041061 None:intergenic 30.0%
! CCTTTACCTAATGGATATTA+TGG - chr3.3:54040912-54040931 MS.gene008570:intron 30.0%
!! AATCATGAATTGTTGATGAC+CGG + chr3.3:54039472-54039491 None:intergenic 30.0%
!! ATTCAAAGAAGCACTTTCAA+AGG - chr3.3:54039520-54039539 MS.gene008570:CDS 30.0%
!! CAAGAAGGAATTGATTTTGA+TGG + chr3.3:54039380-54039399 None:intergenic 30.0%
!! CACCATTGTTGTTAAGTTTT+AGG + chr3.3:54040839-54040858 None:intergenic 30.0%
!! GATTTTGATCCTTTACCTAA+TGG - chr3.3:54040903-54040922 MS.gene008570:intron 30.0%
!!! TGTTTTTAGTCTCTCTTATC+AGG + chr3.3:54041013-54041032 None:intergenic 30.0%
AAGGTGACAAAATTTCGTTG+CGG - chr3.3:54040441-54040460 MS.gene008570:intron 35.0%
AAGTTAGTGAGCTCAATGAA+AGG - chr3.3:54040967-54040986 MS.gene008570:intron 35.0%
ATACATGAACAGTTTGAGCA+AGG + chr3.3:54039448-54039467 None:intergenic 35.0%
ATGATCCATCAATGAAAGGA+GGG - chr3.3:54041276-54041295 MS.gene008570:CDS 35.0%
ATGCTTTGCAATTTCTGTGT+CGG + chr3.3:54040379-54040398 None:intergenic 35.0%
CAACAGATTCCTTGTTCATA+GGG + chr3.3:54040641-54040660 None:intergenic 35.0%
CCAAGTAACAAACAGAAGAA+AGG + chr3.3:54040350-54040369 None:intergenic 35.0%
CGAATTATAACGAGGAATCA+AGG - chr3.3:54041081-54041100 MS.gene008570:intron 35.0%
CTCAAACTGTTCATGTATAC+CGG - chr3.3:54039450-54039469 MS.gene008570:CDS 35.0%
GCCCTAAAACTTAACAACAA+TGG - chr3.3:54040834-54040853 MS.gene008570:intron 35.0%
GCTTCTTTGAATACATGACA+AGG + chr3.3:54039512-54039531 None:intergenic 35.0%
TCAATTTCCTCCTTGAACTT+GGG + chr3.3:54041338-54041357 None:intergenic 35.0%
TCGGTCCTTAAAAATGCTAA+AGG - chr3.3:54039938-54039957 MS.gene008570:CDS 35.0%
TGAAGACAAAACTTACCCTT+TGG - chr3.3:54040413-54040432 MS.gene008570:CDS 35.0%
TTAGCAATTGCTTCCAAGAA+TGG + chr3.3:54040321-54040340 None:intergenic 35.0%
TTCAATTTCCTCCTTGAACT+TGG + chr3.3:54041339-54041358 None:intergenic 35.0%
TTGACAGAGTGGAAACATTT+AGG - chr3.3:54041131-54041150 MS.gene008570:CDS 35.0%
TTGAGTTTGATGAAGGAAAG+TGG - chr3.3:54040750-54040769 MS.gene008570:intron 35.0%
! CACAGAAATTGCAAAGCATT+TGG - chr3.3:54040380-54040399 MS.gene008570:CDS 35.0%
! CCTTTCTTCTGTTTGTTACT+TGG - chr3.3:54040347-54040366 MS.gene008570:CDS 35.0%
! CTTGATGGAGATGTAGATTT+TGG - chr3.3:54041158-54041177 MS.gene008570:CDS 35.0%
!! GCTTAAGTTGAGTTTGATGA+AGG - chr3.3:54040743-54040762 MS.gene008570:intron 35.0%
AAACCTAGTATACCTGCAAG+AGG + chr3.3:54039563-54039582 None:intergenic 40.0%
AACATATCTCTACGTCTTGC+AGG - chr3.3:54040222-54040241 MS.gene008570:CDS 40.0%
AAGCCATTATCGAACTTGCA+AGG - chr3.3:54040532-54040551 MS.gene008570:intron 40.0%
AGCCATTATCGAACTTGCAA+GGG - chr3.3:54040533-54040552 MS.gene008570:intron 40.0%
ATGGTGAAACATGCTGATGA+TGG - chr3.3:54040249-54040268 MS.gene008570:CDS 40.0%
CTGTAGAGTTGTCTTGATTC+AGG + chr3.3:54040677-54040696 None:intergenic 40.0%
GAATCAAGGATGCTAAGTAC+TGG - chr3.3:54041095-54041114 MS.gene008570:intron 40.0%
GAGTTGTCTTGATTCAGGTA+AGG + chr3.3:54040672-54040691 None:intergenic 40.0%
GATGATCCATCAATGAAAGG+AGG - chr3.3:54041275-54041294 MS.gene008570:CDS 40.0%
GCAACAGATTCCTTGTTCAT+AGG + chr3.3:54040642-54040661 None:intergenic 40.0%
GTTGGAGACGAATTATAACG+AGG - chr3.3:54041073-54041092 MS.gene008570:intron 40.0%
TATCCTCTTGCAGGTATACT+AGG - chr3.3:54039557-54039576 MS.gene008570:CDS 40.0%
TGATCCATCAATGAAAGGAG+GGG - chr3.3:54041277-54041296 MS.gene008570:CDS 40.0%
TGGAGGGTGAAATGATAACA+AGG - chr3.3:54040721-54040740 MS.gene008570:intron 40.0%
TGGGAAAGAGAGAGACTTAT+TGG - chr3.3:54040585-54040604 MS.gene008570:intron 40.0%
TGGTATTAGACGCAACTTGA+AGG - chr3.3:54040881-54040900 MS.gene008570:intron 40.0%
TTAACCCCTCCTTTCATTGA+TGG + chr3.3:54041284-54041303 None:intergenic 40.0%
TTGTCACCTTGAACACCAAA+GGG + chr3.3:54040431-54040450 None:intergenic 40.0%
TTTGTCACCTTGAACACCAA+AGG + chr3.3:54040432-54040451 None:intergenic 40.0%
! AGGCATACACCCTCTTTTAA+AGG + chr3.3:54039691-54039710 None:intergenic 40.0%
! GATGGAGATGTAGATTTTGG+TGG - chr3.3:54041161-54041180 MS.gene008570:CDS 40.0%
! TTCATAGGGCATTCTGGAAA+TGG + chr3.3:54040627-54040646 None:intergenic 40.0%
!! AACTTACCCTTTGGTGTTCA+AGG - chr3.3:54040422-54040441 MS.gene008570:CDS 40.0%
AAGCGATTGCAGGTTATGTG+TGG - chr3.3:54040796-54040815 MS.gene008570:intron 45.0%
ACAACTTTCGAAGCGATTGC+AGG - chr3.3:54040786-54040805 MS.gene008570:intron 45.0%
AGGTTTCACAATCGGAATGG+GGG - chr3.3:54040464-54040483 MS.gene008570:intron 45.0%
ATGGACGCAAACAAGTTGCA+TGG + chr3.3:54041215-54041234 None:intergenic 45.0%
GAGGTTTCACAATCGGAATG+GGG - chr3.3:54040463-54040482 MS.gene008570:intron 45.0%
GCCATTATCGAACTTGCAAG+GGG - chr3.3:54040534-54040553 MS.gene008570:intron 45.0%
GCGTCTAATACCAGCTACAA+TGG + chr3.3:54040874-54040893 None:intergenic 45.0%
GGAGGTTTCACAATCGGAAT+GGG - chr3.3:54040462-54040481 MS.gene008570:intron 45.0%
GTGACAAAATTTCGTTGCGG+AGG - chr3.3:54040444-54040463 MS.gene008570:intron 45.0%
TCCAGAATGCCCTATGAACA+AGG - chr3.3:54040629-54040648 MS.gene008570:intron 45.0%
TCCCCTTGCAAGTTCGATAA+TGG + chr3.3:54040538-54040557 None:intergenic 45.0%
TCTACGTCTTGCAGGTAGAA+TGG - chr3.3:54040230-54040249 MS.gene008570:CDS 45.0%
! ATGCAACTTGTTTGCGTCCA+TGG - chr3.3:54041214-54041233 MS.gene008570:CDS 45.0%
! TCCTTGTTCATAGGGCATTC+TGG + chr3.3:54040633-54040652 None:intergenic 45.0%
!! CAGTAACGGTTTTGACAGAG+TGG - chr3.3:54041120-54041139 MS.gene008570:intron 45.0%
!! GCTAAGTACTGGTGCAGTAA+CGG - chr3.3:54041106-54041125 MS.gene008570:intron 45.0%
AGTCTCTCTCTTTCCCACAC+AGG + chr3.3:54040582-54040601 None:intergenic 50.0%
CGATTGCAGGTTATGTGTGG+AGG - chr3.3:54040799-54040818 MS.gene008570:intron 50.0%
CGGAGGTTTCACAATCGGAA+TGG - chr3.3:54040461-54040480 MS.gene008570:intron 50.0%
CGTTGCGGAGGTTTCACAAT+CGG - chr3.3:54040456-54040475 MS.gene008570:intron 50.0%
GCAAACAAGTTGCATGGTGC+TGG + chr3.3:54041209-54041228 None:intergenic 50.0%
GTGTTGCACGCTATCTGTGA+CGG - chr3.3:54040486-54040505 MS.gene008570:intron 50.0%
TTCCTCCTTGAACTTGGGCA+TGG + chr3.3:54041333-54041352 None:intergenic 50.0%
!! ACAGTGCTGACCATTGTAGC+TGG - chr3.3:54040861-54040880 MS.gene008570:intron 50.0%
AACCCTCGACGATACCTGTG+TGG - chr3.3:54040565-54040584 MS.gene008570:intron 55.0%
AACTTGGGCATGGCAGCAAC+AGG + chr3.3:54041323-54041342 None:intergenic 55.0%
ACCCTCGACGATACCTGTGT+GGG - chr3.3:54040566-54040585 MS.gene008570:intron 55.0%
ACTTGGGCATGGCAGCAACA+GGG + chr3.3:54041322-54041341 None:intergenic 55.0%
TGCCATGCCCAAGTTCAAGG+AGG - chr3.3:54041328-54041347 MS.gene008570:CDS 55.0%
TGCTGCCATGCCCAAGTTCA+AGG - chr3.3:54041325-54041344 MS.gene008570:CDS 55.0%
! CACGCTATCTGTGACGGAGT+TGG - chr3.3:54040492-54040511 MS.gene008570:intron 55.0%
! TCCCACACAGGTATCGTCGA+GGG + chr3.3:54040570-54040589 None:intergenic 55.0%
! TTCCCACACAGGTATCGTCG+AGG + chr3.3:54040571-54040590 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 54039320 54041379 54039320 ID=MS.gene008570
chr3.3 mRNA 54039320 54041379 54039320 ID=MS.gene008570.t1;Parent=MS.gene008570
chr3.3 exon 54041130 54041379 54041130 ID=MS.gene008570.t1.exon1;Parent=MS.gene008570.t1
chr3.3 CDS 54041130 54041379 54041130 ID=cds.MS.gene008570.t1;Parent=MS.gene008570.t1
chr3.3 exon 54039320 54040455 54039320 ID=MS.gene008570.t1.exon2;Parent=MS.gene008570.t1
chr3.3 CDS 54039320 54040455 54039320 ID=cds.MS.gene008570.t1;Parent=MS.gene008570.t1
Gene Sequence

>MS.gene008570

ATGGCTTATCAGACAGCTACCCTAAAGCTTGAAATGAAAGATGTTGTGCTCATAAAACCATCAAAATCAATTCCTTCTTGTATTCTTTCACTTTCTACAATTGACAATAGAGAAATCTATAATAACCTTGCTCAAACTGTTCATGTATACCGGTCATCAACAATTCATGATTCTAATTCGGGTTTCGACCCTTGTCATGTATTCAAAGAAGCACTTTCAAAGGCTTTGTTTTATTATTATCCTCTTGCAGGTATACTAGGTTTTTTTTTTTTTCCTTTCATTAAATACAAATGTTTCAAACTAGTGTTGTTAAAATATGGATGCCTCTATCTACCAAAAAAAATAAATATGGATGCCTCTAATATAAACCTTTAAAAGAGGGTGTATGCCTAATCATTTTTTTTCTTATAAATTTTAATAAAAATTTTGGGTTAGAAATATATTTTTTTAGATGAGGTTAGATATATTTTTGGTCCTTATAAATATTCAAAATTTCATTATTTCCTATAAACTAAAATCATAATTTTTAATACCTTTAAAATTTTATGTCAACATTTATAGTCCTTTTAAAATTTTCCATTAGTATTTTTAGTCCTTGTAAAATTTATGTTAGCATTTTCGGTCCTTAAAAATGCTAAAGGAAAATATTACAGAAACTAAAATGTGATTTTATTTTGTAAGGTCAAAAAACAAAATTGTAAATATTTATAGAGACAGAAAAACATATTTAACCCAAAATATGATAGTCTTTTTTTTTTTTTTTGGTACAATGATAGTCTTCTTTTAGTTATAGAAAAATCCATTATTTTACGAAAAATTGCAATGGTCCACTTTTGAATATTTTATGAAAGTCATTATTAGAATGTCCATAAAAATGGATTTTATGGAACACTAAACTAATTAACATATCTCTACGTCTTGCAGGTAGAATGGTGAAACATGCTGATGATGGAAAACTTAGAGTCAATTGCAATCCTAATGCAGAAAATTATGGTGTTCCATTCTTGGAAGCAATTGCTAACTGCACCCTTTCTTCTGTTTGTTACTTGGACAATACCGACACAGAAATTGCAAAGCATTTGGTGCTTGATCTTGAAGACAAAACTTACCCTTTGGTGTTCAAGGTGACAAAATTTCGTTGCGGAGGTTTCACAATCGGAATGGGGGTGTTGCACGCTATCTGTGACGGAGTTGGTGCATCTCAATTCTTCAAAGCCATTATCGAACTTGCAAGGGGAAGAAATGAACCCTCGACGATACCTGTGTGGGAAAGAGAGAGACTTATTGGATCAATAACTAAACAACCATTTCCAGAATGCCCTATGAACAAGGAATCTGTTGCATTTTCACCTTACCTGAATCAAGACAACTCTACAGTTATGAAGAAATATTGTTTTAATATGGAGGGTGAAATGATAACAAGGCTTAAGTTGAGTTTGATGAAGGAAAGTGGAAATATAAGGTTCACAACTTTCGAAGCGATTGCAGGTTATGTGTGGAGGTCAAGAGCAAGAGCCCTAAAACTTAACAACAATGGTGAAACAGTGCTGACCATTGTAGCTGGTATTAGACGCAACTTGAAGGATTTTGATCCTTTACCTAATGGATATTATGGGAATTCATGTGTGGATGCAAACGCTGTGTTAAAAGTTAGTGAGCTCAATGAAAGGCCACTCTATGAAATTATTAACCTGATAAGAGAGACTAAAAACATTGCTTCCACCACTGATTTTGTCAAAAATTCAATCAACACGTTGGAGACGAATTATAACGAGGAATCAAGGATGCTAAGTACTGGTGCAGTAACGGTTTTGACAGAGTGGAAACATTTAGGTTTTCTTGATGGAGATGTAGATTTTGGTGGACATGAAGCAGTTAATTTAGTACCAGCACCATGCAACTTGTTTGCGTCCATGGAAATGAGCATTTTTACACCTCCTAATAAATTTGACAATGATGATCCATCAATGAAAGGAGGGGTTAAGCTTTTTACTACACTCCCTGTTGCTGCCATGCCCAAGTTCAAGGAGGAAATTGAAGCTTTAAGATTTCTTAGTTAA
Protein sequence

>MS.gene008570.t1

MAYQTATLKLEMKDVVLIKPSKSIPSCILSLSTIDNREIYNNLAQTVHVYRSSTIHDSNSGFDPCHVFKEALSKALFYYYPLAGRMVKHADDGKLRVNCNPNAENYGVPFLEAIANCTLSSVCYLDNTDTEIAKHLVLDLEDKTYPLVFKVTKFRCGGFTIGMGVLHAICDGVGASQFFKAIIELARGRNEPSTIPVWERERLIGSITKQPFPECPMNKESVAFSPYLNQDNSTVMKKYCFNMEGEMITRLKLSLMKESGNIRFTTFEAIAGYVWRSRARALKLNNNGETVLTIVAGIRRNLKDFDPLPNGYYGNSCVDANAVLKVSELNERPLYEIINLIRETKNIASTTDFVKNSINTLETNYNEESRMLSTGAVTVLTEWKHLGFLDGDVDFGGHEAVNLVPAPCNLFASMEMSIFTPPNKFDNDDPSMKGGVKLFTTLPVAAMPKFKEEIEALRFLS