AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene011798

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011798.t1 MTR_4g094835 91.051 447 39 1 1 447 1 446 0.0 847
MS.gene011798.t1 MTR_4g094838 80.667 450 65 4 4 448 1 433 0.0 719
MS.gene011798.t1 MTR_4g094832 86.133 375 48 1 78 448 57 431 0.0 666
MS.gene011798.t1 MTR_4g094842 59.471 454 163 9 1 443 1 444 0.0 531
MS.gene011798.t1 MTR_8g041770 58.259 448 171 7 1 443 1 437 0.0 520
MS.gene011798.t1 MTR_0190s0020 56.793 449 177 8 1 443 1 438 1.72e-176 501
MS.gene011798.t1 MTR_8g079710 57.079 445 175 10 1 438 1 436 2.12e-170 486
MS.gene011798.t1 MTR_4g022720 55.333 450 175 8 1 443 1 431 8.35e-169 481
MS.gene011798.t1 MTR_4g022825 53.846 455 179 9 1 443 1 436 4.77e-166 474
MS.gene011798.t1 MTR_4g094828 54.084 453 183 11 1 439 1 442 5.13e-166 475
MS.gene011798.t1 MTR_4g022290 54.788 449 181 10 1 439 1 437 2.86e-163 468
MS.gene011798.t1 MTR_3g088420 53.675 449 182 9 1 443 1 429 6.66e-162 464
MS.gene011798.t1 MTR_4g022670 52.412 456 185 8 1 443 1 437 2.12e-161 463
MS.gene011798.t1 MTR_4g022800 53.392 457 180 9 1 443 1 438 4.41e-161 462
MS.gene011798.t1 MTR_3g026310 51.404 463 186 9 1 443 1 444 2.35e-158 455
MS.gene011798.t1 MTR_7g026860 51.316 456 187 9 1 443 1 434 2.75e-154 445
MS.gene011798.t1 MTR_4g022760 46.491 456 168 8 1 443 1 393 1.08e-131 385
MS.gene011798.t1 MTR_6g016815 41.964 448 170 13 1 443 15 377 8.19e-102 309
MS.gene011798.t1 MTR_3g101900 41.943 422 228 8 32 443 30 444 1.40e-99 305
MS.gene011798.t1 MTR_3g101930 40.783 434 231 9 28 443 26 451 4.53e-98 301
MS.gene011798.t1 MTR_3g102020 38.646 458 249 10 3 443 5 447 9.13e-95 293
MS.gene011798.t1 MTR_3g101960 38.816 456 248 10 3 442 5 445 2.78e-93 289
MS.gene011798.t1 MTR_3g102010 40.698 430 233 8 28 443 26 447 3.29e-93 289
MS.gene011798.t1 MTR_3g101970 38.876 427 232 8 28 443 25 433 3.23e-91 283
MS.gene011798.t1 MTR_4g094825 74.479 192 47 2 19 210 80 269 4.59e-87 271
MS.gene011798.t1 MTR_4g094825 63.314 169 47 2 285 448 234 392 8.40e-66 216
MS.gene011798.t1 MTR_3g070170 35.652 460 250 8 22 447 23 470 1.03e-83 265
MS.gene011798.t1 MTR_8g040780 58.491 212 81 3 234 442 20 227 1.79e-78 244
MS.gene011798.t1 MTR_5g081960 32.919 483 256 8 22 448 17 487 3.39e-77 249
MS.gene011798.t1 MTR_4g015180 34.238 479 242 10 24 443 19 483 8.30e-76 245
MS.gene011798.t1 MTR_8g040760 52.466 223 94 5 1 220 1 214 1.29e-71 225
MS.gene011798.t1 MTR_7g026850 53.846 156 67 2 37 192 12 162 2.55e-53 177
MS.gene011798.t1 MTR_6g045553 29.087 471 260 13 23 445 29 473 1.46e-50 178
MS.gene011798.t1 MTR_3g102030 32.673 303 170 4 103 401 48 320 1.63e-46 163
MS.gene011798.t1 MTR_6g034485 59.690 129 46 2 199 325 1 125 3.82e-46 156
MS.gene011798.t1 MTR_7g026770 47.222 180 71 4 154 331 132 289 8.37e-43 153
MS.gene011798.t1 MTR_6g034495 57.627 118 49 1 75 192 45 161 7.18e-42 146
MS.gene011798.t1 MTR_7g026830 65.432 81 28 0 97 177 16 96 2.53e-32 118
MS.gene011798.t1 MTR_4g007540 25.316 395 249 14 64 436 56 426 2.06e-29 119
MS.gene011798.t1 MTR_3g098980 26.974 456 252 18 18 430 3 420 2.40e-29 119
MS.gene011798.t1 MTR_8g075610 24.235 392 253 14 64 436 47 413 8.65e-27 112
MS.gene011798.t1 MTR_2g064495 23.177 384 254 12 64 428 56 417 3.34e-25 107
MS.gene011798.t1 MTR_2g105330 23.177 384 254 12 64 428 56 417 3.82e-25 107
MS.gene011798.t1 MTR_8g096530 28.090 356 215 11 57 396 52 382 8.49e-25 106
MS.gene011798.t1 MTR_3g026950 24.943 441 254 16 38 432 28 437 1.12e-23 103
MS.gene011798.t1 MTR_4g097090 23.664 393 255 11 64 428 65 440 5.47e-22 98.6
MS.gene011798.t1 MTR_3g049150 24.631 406 262 15 56 432 43 433 2.05e-21 96.7
MS.gene011798.t1 MTR_7g014250 24.667 450 271 18 32 438 26 450 2.12e-21 96.7
MS.gene011798.t1 MTR_4g127650 22.959 392 258 10 64 428 66 440 2.63e-21 96.3
MS.gene011798.t1 MTR_2g089765 28.051 467 251 23 22 431 29 467 1.21e-20 94.7
MS.gene011798.t1 MTR_3g086020 26.396 394 243 17 64 448 61 416 1.54e-20 93.6
MS.gene011798.t1 MTR_4g055170 28.383 303 190 10 64 355 69 355 2.15e-20 93.6
MS.gene011798.t1 MTR_8g079970 24.029 412 252 13 33 413 17 398 2.28e-20 93.6
MS.gene011798.t1 MTR_7g026800 37.805 164 68 6 223 384 29 160 2.46e-20 89.0
MS.gene011798.t1 MTR_7g014330 24.402 418 255 16 32 409 26 422 3.55e-20 93.2
MS.gene011798.t1 MTR_4g022780 36.747 166 71 6 223 386 30 163 2.35e-19 85.9
MS.gene011798.t1 MTR_7g026780 43.519 108 35 2 336 443 26 107 4.04e-19 83.2
MS.gene011798.t1 MTR_7g027175 23.389 419 240 15 52 434 56 429 6.73e-19 89.0
MS.gene011798.t1 MTR_3g028210 24.731 465 286 19 4 428 8 448 1.34e-18 88.2
MS.gene011798.t1 MTR_7g014230 24.286 420 257 16 30 409 26 424 2.27e-18 87.4
MS.gene011798.t1 MTR_1g081660 22.876 459 273 19 32 447 31 451 2.88e-18 87.0
MS.gene011798.t1 MTR_6g015850 25.342 438 276 16 30 436 32 449 4.04e-18 86.7
MS.gene011798.t1 MTR_4g013050 23.951 405 239 12 57 438 46 404 4.05e-18 86.3
MS.gene011798.t1 MTR_8g012750 24.891 458 272 17 29 434 23 460 4.85e-18 86.7
MS.gene011798.t1 MTR_6g015315 23.928 443 256 16 37 437 42 445 5.48e-18 86.3
MS.gene011798.t1 MTR_1g053315 25.210 357 220 16 64 397 78 410 6.28e-18 86.3
MS.gene011798.t1 MTR_6g015320 24.722 449 259 15 32 434 29 444 6.82e-18 86.3
MS.gene011798.t1 MTR_3g071320 25.217 345 221 12 29 352 23 351 2.92e-17 84.0
MS.gene011798.t1 MTR_1g040195 24.474 380 243 13 74 434 51 405 3.64e-17 83.6
MS.gene011798.t1 MTR_7g084940 26.039 361 226 14 52 396 57 392 5.46e-17 83.2
MS.gene011798.t1 MTR_6g015815 23.864 440 283 15 30 436 32 452 5.96e-17 83.2
MS.gene011798.t1 MTR_7g085930 24.198 405 261 13 61 432 56 447 2.33e-16 81.6
MS.gene011798.t1 MTR_2g089670 26.292 445 260 21 30 431 43 462 7.35e-16 80.1
MS.gene011798.t1 MTR_5g084850 23.503 451 266 19 33 447 17 424 1.61e-15 78.6
MS.gene011798.t1 MTR_5g084870 23.704 405 246 16 33 408 17 387 2.16e-15 78.2
MS.gene011798.t1 MTR_1g040175 22.628 411 273 16 48 436 45 432 2.36e-15 78.2
MS.gene011798.t1 MTR_7g015860 23.913 414 249 15 58 431 58 445 2.46e-15 78.2
MS.gene011798.t1 MTR_7g014610 24.190 463 271 20 22 428 19 457 2.73e-15 78.2
MS.gene011798.t1 MTR_3g087640 26.225 408 252 18 64 434 67 462 3.83e-15 77.8
MS.gene011798.t1 MTR_7g014200 25.721 451 257 20 30 429 28 451 4.47e-15 77.4
MS.gene011798.t1 MTR_7g084910 25.430 291 184 8 52 326 58 331 1.06e-14 76.3
MS.gene011798.t1 MTR_3g049140 24.786 234 154 6 213 432 5 230 1.07e-14 74.3
MS.gene011798.t1 MTR_7g014160 23.947 451 264 18 32 428 30 455 1.97e-14 75.5
MS.gene011798.t1 MTR_8g079980 23.077 377 227 13 59 409 54 393 2.95e-14 74.7
MS.gene011798.t1 MTR_2g007880 24.324 407 253 16 64 446 74 449 2.96e-14 75.1
MS.gene011798.t1 MTR_7g085990 25.124 402 253 15 64 431 55 442 4.31e-14 74.3
MS.gene011798.t1 MTR_3g465980 24.911 281 189 8 59 329 63 331 7.59e-14 73.6
MS.gene011798.t1 MTR_5g076700 20.320 438 298 13 33 441 24 439 8.20e-14 73.6
MS.gene011798.t1 MTR_5g026320 22.794 408 259 14 61 442 71 448 8.84e-14 73.6
MS.gene011798.t1 MTR_2g089735 25.000 412 254 16 64 438 62 455 3.21e-13 71.6
MS.gene011798.t1 MTR_1g094790 26.207 435 261 20 40 443 32 437 3.86e-13 71.2
MS.gene011798.t1 MTR_7g114520 21.117 412 275 15 54 441 56 441 6.37e-13 70.9
MS.gene011798.t1 MTR_8g079950 23.333 450 275 18 33 447 17 431 1.20e-12 70.1
MS.gene011798.t1 MTR_7g056087 22.278 395 250 16 64 430 58 423 1.54e-12 69.7
MS.gene011798.t1 MTR_7g114490 21.818 440 274 17 33 434 24 431 1.55e-12 69.7
MS.gene011798.t1 MTR_7g013850 22.066 426 256 14 37 406 37 442 2.44e-12 68.9
MS.gene011798.t1 MTR_7g026870 50.704 71 28 3 291 355 82 151 3.02e-12 64.7
MS.gene011798.t1 MTR_3g465910 26.280 293 180 10 59 334 63 336 4.67e-12 68.2
MS.gene011798.t1 MTR_7g026880 56.000 50 20 1 394 443 2 49 2.02e-11 59.3
MS.gene011798.t1 MTR_3g465990 24.468 282 189 10 59 329 63 331 2.07e-11 66.2
MS.gene011798.t1 MTR_2g015430 21.203 349 242 10 64 399 58 386 8.93e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011798.t1 AT5G67150 41.850 454 242 8 2 445 3 444 1.48e-118 354
MS.gene011798.t1 AT3G50300 41.410 454 244 10 1 443 1 443 1.57e-107 326
MS.gene011798.t1 AT3G50280 39.024 451 255 8 1 443 1 439 3.07e-102 312
MS.gene011798.t1 AT5G67160 38.839 448 244 10 2 442 3 427 7.31e-101 308
MS.gene011798.t1 AT3G50270 37.500 456 256 12 4 445 6 446 6.79e-98 301
MS.gene011798.t1 AT5G38130 39.954 438 239 13 1 429 21 443 1.06e-95 296
MS.gene011798.t1 AT5G07850 38.318 428 239 10 32 443 31 449 6.10e-87 273
MS.gene011798.t1 AT5G42830 37.089 426 244 9 32 443 30 445 2.87e-86 271
MS.gene011798.t1 AT2G39980 33.476 466 246 7 30 445 27 478 8.49e-83 263
MS.gene011798.t1 AT5G07860 37.617 428 238 10 32 443 31 445 9.92e-82 259
MS.gene011798.t1 AT5G01210 34.698 464 252 8 24 448 19 470 2.34e-78 251
MS.gene011798.t1 AT3G50280 40.836 311 177 4 138 443 1 309 1.69e-75 239
MS.gene011798.t1 AT5G07870 35.991 439 242 11 32 443 31 457 2.19e-74 241
MS.gene011798.t1 AT3G50290 41.791 268 146 4 135 394 4 269 5.01e-62 202
MS.gene011798.t1 AT5G23940 33.411 431 263 10 32 444 25 449 1.53e-60 205
MS.gene011798.t1 AT3G29590 27.126 435 283 14 25 438 21 442 5.12e-28 115
MS.gene011798.t1 AT5G48930 24.936 393 243 15 64 431 56 421 2.49e-26 110
MS.gene011798.t1 AT2G19070 24.891 458 278 17 24 436 7 443 9.47e-25 106
MS.gene011798.t1 AT5G41040 25.253 396 243 13 57 430 78 442 2.06e-24 105
MS.gene011798.t1 AT5G41040 25.253 396 243 13 57 430 62 426 2.32e-24 105
MS.gene011798.t1 AT3G29670 24.485 437 291 15 15 428 13 433 6.16e-24 104
MS.gene011798.t1 AT5G39080 24.444 450 275 17 30 436 26 453 2.12e-22 99.8
MS.gene011798.t1 AT3G29635 24.306 432 276 15 29 428 26 438 6.22e-22 98.2
MS.gene011798.t1 AT5G39090 26.744 430 260 21 32 431 28 432 1.20e-21 97.4
MS.gene011798.t1 AT3G48720 26.265 415 256 19 45 436 40 427 2.04e-21 96.7
MS.gene011798.t1 AT2G40230 24.700 417 275 11 29 423 23 422 6.95e-21 95.1
MS.gene011798.t1 AT3G30280 25.056 443 282 20 26 447 23 436 8.97e-21 94.7
MS.gene011798.t1 AT5G39050 25.000 328 208 13 135 431 132 452 1.17e-19 91.7
MS.gene011798.t1 AT1G24430 25.490 408 256 14 54 447 52 425 1.57e-19 90.9
MS.gene011798.t1 AT5G57840 24.936 389 246 12 64 428 56 422 2.68e-18 87.4
MS.gene011798.t1 AT5G07080 27.508 309 180 12 42 327 44 331 3.21e-18 87.0
MS.gene011798.t1 AT5G63560 24.173 393 243 16 57 428 54 412 5.55e-18 86.3
MS.gene011798.t1 AT3G26040 24.105 419 259 16 52 447 47 429 9.23e-18 85.5
MS.gene011798.t1 AT1G28680 24.355 349 230 13 64 399 62 389 1.13e-16 82.4
MS.gene011798.t1 AT1G03940 25.243 412 248 19 58 431 60 449 1.14e-16 82.4
MS.gene011798.t1 AT3G03480 23.227 409 259 17 33 416 33 411 4.36e-16 80.5
MS.gene011798.t1 AT3G47170 23.734 316 204 9 64 357 70 370 8.39e-16 79.7
MS.gene011798.t1 AT1G24420 25.225 444 274 18 26 447 22 429 1.22e-15 79.3
MS.gene011798.t1 AT1G24420 25.225 444 274 18 26 447 22 429 1.22e-15 79.3
MS.gene011798.t1 AT5G61160 22.297 444 286 19 26 434 19 438 5.89e-15 77.0
MS.gene011798.t1 AT1G65450 23.839 323 206 13 52 355 60 361 6.40e-15 77.0
MS.gene011798.t1 AT5G47950 23.982 442 273 20 25 447 22 419 1.96e-14 75.5
MS.gene011798.t1 AT1G28680 23.746 299 203 9 64 352 62 345 2.17e-14 75.1
MS.gene011798.t1 AT1G31490 24.413 426 238 18 61 448 59 438 4.13e-14 74.3
MS.gene011798.t1 AT5G47980 23.171 410 257 16 63 447 60 436 2.28e-13 72.0
MS.gene011798.t1 AT2G25150 22.905 358 234 11 64 400 67 403 4.15e-13 71.6
MS.gene011798.t1 AT1G32910 21.763 363 227 10 64 397 65 399 1.65e-11 66.6
MS.gene011798.t1 AT2G23510 22.767 347 242 12 64 399 69 400 1.92e-11 66.2

Find 87 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGCTTGACGAGGATTATTT+TGG 0.141275 8.1:+26741609 MS.gene011798:CDS
AAGGAACATAGTTTGGTTTA+AGG 0.151696 8.1:-26741062 None:intergenic
TCTCCGAGGTTTGATGTTTA+TGG 0.199950 8.1:+26741837 MS.gene011798:CDS
GTAAAATGGAAAATTCTTTC+AGG 0.213962 8.1:-26741393 None:intergenic
CACATTCTGATGAGAAAATA+AGG 0.214956 8.1:+26741730 MS.gene011798:CDS
CGTTGAAATGAAGGGAGTTT+TGG 0.257934 8.1:-26741285 None:intergenic
GATTTGAACATTCTCTGTTT+TGG 0.269213 8.1:-26741460 None:intergenic
GGGAAATGATGCTGAGTTCT+TGG 0.295386 8.1:+26742004 MS.gene011798:CDS
TCAAATGGTGGATTTGGTTT+TGG 0.300399 8.1:-26740867 None:intergenic
GATGTTTATGGTAATGATTT+TGG 0.307637 8.1:+26741849 MS.gene011798:CDS
GCCTGTTAAGGGTGGAAAAT+AGG 0.317928 8.1:-26740914 None:intergenic
ACGTAATGAAAATCTTCTTC+TGG 0.318483 8.1:-26741552 None:intergenic
GTTGTTTGGGATTAATAGTT+TGG 0.327840 8.1:+26741785 MS.gene011798:CDS
TTTGTTTCAAATGGTGGATT+TGG 0.337767 8.1:-26740873 None:intergenic
ACTATAGAAGGAACATAGTT+TGG 0.352065 8.1:-26741069 None:intergenic
TGATGTGTTGTTGTTGTTTG+TGG 0.358976 8.1:-26740965 None:intergenic
CCACTTGAAACCATCCAAAA+AGG 0.359472 8.1:+26740817 MS.gene011798:CDS
GATTATAAGACGGCCTGTTA+AGG 0.359476 8.1:-26740926 None:intergenic
TTTGAGATATTGGAGGCTTT+GGG 0.368507 8.1:+26741984 MS.gene011798:CDS
GGGAAGATTACTGTGTTTGC+AGG 0.373043 8.1:+26741921 MS.gene011798:CDS
ATTGGAAGTTCAACGTCGTT+TGG 0.373841 8.1:-26741315 None:intergenic
TTTGTGATGGCAAGATGTTT+TGG 0.386713 8.1:+26741214 MS.gene011798:CDS
TTGAGAATGCCGAAGTTGTT+TGG 0.387603 8.1:+26741771 MS.gene011798:CDS
TTAGCTAATGGCATCTTCAT+TGG 0.396826 8.1:+26741171 MS.gene011798:CDS
CATTAGCTAACTCCGTTACT+TGG 0.402418 8.1:-26741158 None:intergenic
TTGGAAGTTCAACGTCGTTT+GGG 0.408058 8.1:-26741314 None:intergenic
TTTGGAAATGCAGGGATCAT+TGG 0.408778 8.1:+26741627 MS.gene011798:CDS
TGAGAATGCCGAAGTTGTTT+GGG 0.411706 8.1:+26741772 MS.gene011798:CDS
TTTGGCAGGTGCTAATTCAT+TGG 0.414867 8.1:+26741803 MS.gene011798:CDS
TGGAAAGTGTTTGTTTCAAA+TGG 0.417515 8.1:-26740882 None:intergenic
TTTGGCATTTCGTCAATACT+TGG 0.430278 8.1:+26741232 MS.gene011798:CDS
TTTGTGAAAGGAAATTGTAT+TGG 0.434338 8.1:-26741333 None:intergenic
TTTGGTTTGGAATGGATTGA+TGG 0.436388 8.1:-26740849 None:intergenic
TTTGGGATTAATAGTTTGGC+AGG 0.440696 8.1:+26741789 MS.gene011798:CDS
TGGGATTGGAAAATGTTCAT+TGG 0.444512 8.1:+26741686 MS.gene011798:CDS
AAGCGTTGTTCTCTCATGTA+TGG 0.446035 8.1:+26741499 MS.gene011798:CDS
GTAGTGTAACTATGAAAGTA+GGG 0.457732 8.1:+26741649 MS.gene011798:CDS
TTGAGATTATTGTTGTCAAT+TGG 0.459438 8.1:-26740739 None:intergenic
GGTAGTGTAACTATGAAAGT+AGG 0.460447 8.1:+26741648 MS.gene011798:CDS
GGAGTGCAAATAAAAGTAAT+GGG 0.465217 8.1:+26741901 MS.gene011798:CDS
CTCCCACTTCGAACCCCTAC+CGG 0.466626 8.1:-26741882 None:intergenic
ATTATAAGACGGCCTGTTAA+GGG 0.469230 8.1:-26740925 None:intergenic
GAATGGATTGATGGAAAAGA+AGG 0.472695 8.1:-26740840 None:intergenic
GGGAACCAACGTTGAAATGA+AGG 0.474068 8.1:-26741294 None:intergenic
GGGAGTGCAAATAAAAGTAA+TGG 0.474744 8.1:+26741900 MS.gene011798:CDS
GTTTGTGGCTACGTTGTGTT+GGG 0.476915 8.1:-26740950 None:intergenic
AAACCGGTAGGGGTTCGAAG+TGG 0.479206 8.1:+26741879 MS.gene011798:CDS
CAAGTAACGGAGTTAGCTAA+TGG 0.482336 8.1:+26741159 MS.gene011798:CDS
AAAGTGTTTGTTTCAAATGG+TGG 0.488128 8.1:-26740879 None:intergenic
ACCAAGGTTGTTGCAAATGA+TGG 0.492052 8.1:-26740992 None:intergenic
GTTACTTGGACTGCTAGAAT+AGG 0.498322 8.1:-26741144 None:intergenic
TGTTTGTGGCTACGTTGTGT+TGG 0.512319 8.1:-26740951 None:intergenic
TTTGGATGGTTTCAAGTGGA+AGG 0.513017 8.1:-26740813 None:intergenic
CTCAACCACGTTGTTTGTGA+TGG 0.514978 8.1:+26741201 MS.gene011798:CDS
GAGATTCTAGAATGTGGGAT+TGG 0.518222 8.1:+26741672 MS.gene011798:CDS
TAGGGGAGATTCTAGAATGT+GGG 0.520801 8.1:+26741667 MS.gene011798:CDS
TCCATCATTTGCAACAACCT+TGG 0.522468 8.1:+26740991 MS.gene011798:CDS
TATTGTTGTCAATTGGTGCT+TGG 0.527153 8.1:-26740732 None:intergenic
GTAGGGGAGATTCTAGAATG+TGG 0.529927 8.1:+26741666 MS.gene011798:CDS
ACATTCTGATGAGAAAATAA+GGG 0.533534 8.1:+26741731 MS.gene011798:CDS
AACCGGTAGGGGTTCGAAGT+GGG 0.542449 8.1:+26741880 MS.gene011798:CDS
TCAAGCGGAGGAATCATCCT+TGG 0.542679 8.1:-26741594 None:intergenic
GATAATCGATTTAACACCAT+GGG 0.544330 8.1:+26740780 MS.gene011798:CDS
AACTCCCTTCATTTCAACGT+TGG 0.545872 8.1:+26741289 MS.gene011798:CDS
CTTTCAGGTAAGTTCTGCGT+CGG 0.565322 8.1:-26741378 None:intergenic
TATCTTCGTCCACGCAAAAG+CGG 0.573385 8.1:+26741017 MS.gene011798:CDS
GGAACCAACGTTGAAATGAA+GGG 0.576282 8.1:-26741293 None:intergenic
GTGTTTGCAGGAGCTGAAGA+AGG 0.577173 8.1:+26741933 MS.gene011798:CDS
CTATTAATCCCAAACAACTT+CGG 0.578183 8.1:-26741780 None:intergenic
CCAAAATAATCCTCGTCAAG+CGG 0.581464 8.1:-26741609 None:intergenic
ACTAATTGAGATCACTGTGA+TGG 0.585183 8.1:-26740704 None:intergenic
TAAGGGATGATTATGAGTGT+TGG 0.592031 8.1:+26741748 MS.gene011798:CDS
TAGTTATAGGTGTTAGACCA+AGG 0.600032 8.1:+26741577 MS.gene011798:CDS
GGAAGGAATTGAAGATCCCA+TGG 0.614918 8.1:-26740796 None:intergenic
TATTCTAGCAGTCCAAGTAA+CGG 0.629197 8.1:+26741146 MS.gene011798:CDS
TGTGTTGGGTGATTATAAGA+CGG 0.629490 8.1:-26740936 None:intergenic
GATGATTCCTCCGCTTGACG+AGG 0.630798 8.1:+26741599 MS.gene011798:CDS
AAATAATCCTCGTCAAGCGG+AGG 0.636490 8.1:-26741606 None:intergenic
AGATAATCGATTTAACACCA+TGG 0.645348 8.1:+26740779 MS.gene011798:CDS
TGCTAGAATAGGTTGTGAAG+TGG 0.647856 8.1:-26741133 None:intergenic
ATAAGACGGCCTGTTAAGGG+TGG 0.648919 8.1:-26740922 None:intergenic
TGGAAAATAGGAGAGTGTTG+TGG 0.662672 8.1:-26740902 None:intergenic
TAGTGTAACTATGAAAGTAG+GGG 0.674189 8.1:+26741650 MS.gene011798:CDS
TTACCATAAACATCAAACCT+CGG 0.674952 8.1:-26741840 None:intergenic
TGGCTACGAGTAGTTCTCCG+AGG 0.676435 8.1:+26741823 MS.gene011798:CDS
CGTGGACGAAGATAGCACCA+AGG 0.735616 8.1:-26741008 None:intergenic
TCTTGCCATCACAAACAACG+TGG 0.787958 8.1:-26741206 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAATTTTCCATTTTACAA+AGG + chr8.1:26741398-26741417 MS.gene011798:CDS 20.0%
!!! TTTCATTACGTTTTAGTTAT+AGG + chr8.1:26741564-26741583 MS.gene011798:CDS 20.0%
! AAAAATGAGTGAACTATAGA+AGG - chr8.1:26741084-26741103 None:intergenic 25.0%
! ACATTCTGATGAGAAAATAA+GGG + chr8.1:26741731-26741750 MS.gene011798:CDS 25.0%
! GTAAAATGGAAAATTCTTTC+AGG - chr8.1:26741396-26741415 None:intergenic 25.0%
! TTGAGATTATTGTTGTCAAT+TGG - chr8.1:26740742-26740761 None:intergenic 25.0%
! TTTGTGAAAGGAAATTGTAT+TGG - chr8.1:26741336-26741355 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr8.1:26741849-26741868 MS.gene011798:CDS 25.0%
!!! TTATGGTAATGATTTTGGTT+GGG + chr8.1:26741854-26741873 MS.gene011798:CDS 25.0%
!!! TTGTCTTTCTTTTGAGATAT+TGG + chr8.1:26741974-26741993 MS.gene011798:CDS 25.0%
!!! TTTATGGTAATGATTTTGGT+TGG + chr8.1:26741853-26741872 MS.gene011798:CDS 25.0%
ACGTAATGAAAATCTTCTTC+TGG - chr8.1:26741555-26741574 None:intergenic 30.0%
ACTATAGAAGGAACATAGTT+TGG - chr8.1:26741072-26741091 None:intergenic 30.0%
CACATTCTGATGAGAAAATA+AGG + chr8.1:26741730-26741749 MS.gene011798:CDS 30.0%
CTATTAATCCCAAACAACTT+CGG - chr8.1:26741783-26741802 None:intergenic 30.0%
GGAGTGCAAATAAAAGTAAT+GGG + chr8.1:26741901-26741920 MS.gene011798:CDS 30.0%
GTAGTGTAACTATGAAAGTA+GGG + chr8.1:26741649-26741668 MS.gene011798:CDS 30.0%
TAGTGTAACTATGAAAGTAG+GGG + chr8.1:26741650-26741669 MS.gene011798:CDS 30.0%
TTACCATAAACATCAAACCT+CGG - chr8.1:26741843-26741862 None:intergenic 30.0%
! AAAGTGTTTGTTTCAAATGG+TGG - chr8.1:26740882-26740901 None:intergenic 30.0%
! AAGGAACATAGTTTGGTTTA+AGG - chr8.1:26741065-26741084 None:intergenic 30.0%
! AGATAATCGATTTAACACCA+TGG + chr8.1:26740779-26740798 MS.gene011798:CDS 30.0%
! CGATTTTCTCCTTTGTAAAA+TGG - chr8.1:26741410-26741429 None:intergenic 30.0%
! GATAATCGATTTAACACCAT+GGG + chr8.1:26740780-26740799 MS.gene011798:CDS 30.0%
! GATTTGAACATTCTCTGTTT+TGG - chr8.1:26741463-26741482 None:intergenic 30.0%
! GTTGTTTGGGATTAATAGTT+TGG + chr8.1:26741785-26741804 MS.gene011798:CDS 30.0%
! TTTTACAAAGGAGAAAATCG+CGG + chr8.1:26741410-26741429 MS.gene011798:CDS 30.0%
!! AGTTTTTAACTCCGTTAAGT+GGG - chr8.1:26741107-26741126 None:intergenic 30.0%
!! TAGTTTTTAACTCCGTTAAG+TGG - chr8.1:26741108-26741127 None:intergenic 30.0%
!! TGGAAAGTGTTTGTTTCAAA+TGG - chr8.1:26740885-26740904 None:intergenic 30.0%
!! TTTGTTTCAAATGGTGGATT+TGG - chr8.1:26740876-26740895 None:intergenic 30.0%
!!! AGGATTATTTTGGAAATGCA+GGG + chr8.1:26741619-26741638 MS.gene011798:CDS 30.0%
!!! TATGGTAATGATTTTGGTTG+GGG + chr8.1:26741855-26741874 MS.gene011798:CDS 30.0%
!!! TCTTTCTTTTGAGATATTGG+AGG + chr8.1:26741977-26741996 MS.gene011798:CDS 30.0%
ACTAATTGAGATCACTGTGA+TGG - chr8.1:26740707-26740726 None:intergenic 35.0%
ATTATAAGACGGCCTGTTAA+GGG - chr8.1:26740928-26740947 None:intergenic 35.0%
GGTAGTGTAACTATGAAAGT+AGG + chr8.1:26741648-26741667 MS.gene011798:CDS 35.0%
TAAGGGATGATTATGAGTGT+TGG + chr8.1:26741748-26741767 MS.gene011798:CDS 35.0%
TAGTTATAGGTGTTAGACCA+AGG + chr8.1:26741577-26741596 MS.gene011798:CDS 35.0%
TATTCTAGCAGTCCAAGTAA+CGG + chr8.1:26741146-26741165 MS.gene011798:CDS 35.0%
TGGGATTGGAAAATGTTCAT+TGG + chr8.1:26741686-26741705 MS.gene011798:CDS 35.0%
TGTGTTGGGTGATTATAAGA+CGG - chr8.1:26740939-26740958 None:intergenic 35.0%
TTAGCTAATGGCATCTTCAT+TGG + chr8.1:26741171-26741190 MS.gene011798:CDS 35.0%
TTTGGCATTTCGTCAATACT+TGG + chr8.1:26741232-26741251 MS.gene011798:CDS 35.0%
! CGGTTTTCTACATTGTGATT+CGG - chr8.1:26741361-26741380 None:intergenic 35.0%
! GGGAGTGCAAATAAAAGTAA+TGG + chr8.1:26741900-26741919 MS.gene011798:CDS 35.0%
! TATTGTTGTCAATTGGTGCT+TGG - chr8.1:26740735-26740754 None:intergenic 35.0%
! TTTGAGATATTGGAGGCTTT+GGG + chr8.1:26741984-26742003 MS.gene011798:CDS 35.0%
! TTTGGGATTAATAGTTTGGC+AGG + chr8.1:26741789-26741808 MS.gene011798:CDS 35.0%
! TTTGTGATGGCAAGATGTTT+TGG + chr8.1:26741214-26741233 MS.gene011798:CDS 35.0%
!! CACTCATTTTTCCCACTTAA+CGG + chr8.1:26741093-26741112 MS.gene011798:CDS 35.0%
!! GAATGGATTGATGGAAAAGA+AGG - chr8.1:26740843-26740862 None:intergenic 35.0%
!! TGATGTGTTGTTGTTGTTTG+TGG - chr8.1:26740968-26740987 None:intergenic 35.0%
!! TTTGGTTTGGAATGGATTGA+TGG - chr8.1:26740852-26740871 None:intergenic 35.0%
!!! ATGGTAATGATTTTGGTTGG+GGG + chr8.1:26741856-26741875 MS.gene011798:CDS 35.0%
!!! GAGGATTATTTTGGAAATGC+AGG + chr8.1:26741618-26741637 MS.gene011798:CDS 35.0%
!!! GATTTGGTTTTGGTTTGGAA+TGG - chr8.1:26740860-26740879 None:intergenic 35.0%
!!! TCAAATGGTGGATTTGGTTT+TGG - chr8.1:26740870-26740889 None:intergenic 35.0%
!!! TGTGATTCGGTTTTTGTGAA+AGG - chr8.1:26741348-26741367 None:intergenic 35.0%
!!! TTTTGAGATATTGGAGGCTT+TGG + chr8.1:26741983-26742002 MS.gene011798:CDS 35.0%
AACTCCCTTCATTTCAACGT+TGG + chr8.1:26741289-26741308 MS.gene011798:CDS 40.0%
ACCAAGGTTGTTGCAAATGA+TGG - chr8.1:26740995-26741014 None:intergenic 40.0%
ATTGGAAGTTCAACGTCGTT+TGG - chr8.1:26741318-26741337 None:intergenic 40.0%
CATTAGCTAACTCCGTTACT+TGG - chr8.1:26741161-26741180 None:intergenic 40.0%
CCAAAATAATCCTCGTCAAG+CGG - chr8.1:26741612-26741631 None:intergenic 40.0%
CCACTTGAAACCATCCAAAA+AGG + chr8.1:26740817-26740836 MS.gene011798:CDS 40.0%
GATTATAAGACGGCCTGTTA+AGG - chr8.1:26740929-26740948 None:intergenic 40.0%
GGAACCAACGTTGAAATGAA+GGG - chr8.1:26741296-26741315 None:intergenic 40.0%
GTTACTTGGACTGCTAGAAT+AGG - chr8.1:26741147-26741166 None:intergenic 40.0%
TCCATCATTTGCAACAACCT+TGG + chr8.1:26740991-26741010 MS.gene011798:CDS 40.0%
TCTCCGAGGTTTGATGTTTA+TGG + chr8.1:26741837-26741856 MS.gene011798:CDS 40.0%
TGAGAATGCCGAAGTTGTTT+GGG + chr8.1:26741772-26741791 MS.gene011798:CDS 40.0%
TGCTAGAATAGGTTGTGAAG+TGG - chr8.1:26741136-26741155 None:intergenic 40.0%
TTGAGAATGCCGAAGTTGTT+TGG + chr8.1:26741771-26741790 MS.gene011798:CDS 40.0%
TTGGAAGTTCAACGTCGTTT+GGG - chr8.1:26741317-26741336 None:intergenic 40.0%
TTTGGAAATGCAGGGATCAT+TGG + chr8.1:26741627-26741646 MS.gene011798:CDS 40.0%
TTTGGCAGGTGCTAATTCAT+TGG + chr8.1:26741803-26741822 MS.gene011798:CDS 40.0%
! AAGCGTTGTTCTCTCATGTA+TGG + chr8.1:26741499-26741518 MS.gene011798:CDS 40.0%
! CAAGTAACGGAGTTAGCTAA+TGG + chr8.1:26741159-26741178 MS.gene011798:CDS 40.0%
! CCTTTTTGGATGGTTTCAAG+TGG - chr8.1:26740820-26740839 None:intergenic 40.0%
! CGTTGAAATGAAGGGAGTTT+TGG - chr8.1:26741288-26741307 None:intergenic 40.0%
! GAAAAGAAGGCCTTTTTGGA+TGG - chr8.1:26740830-26740849 None:intergenic 40.0%
! GATGGAAAAGAAGGCCTTTT+TGG - chr8.1:26740834-26740853 None:intergenic 40.0%
! TCCTATTTTCCACCCTTAAC+AGG + chr8.1:26740913-26740932 MS.gene011798:CDS 40.0%
! TTTGGATGGTTTCAAGTGGA+AGG - chr8.1:26740816-26740835 None:intergenic 40.0%
!! AAGGGAGTTTTGGTATACTC+TGG - chr8.1:26741278-26741297 None:intergenic 40.0%
!! AGGGAGTTTTGGTATACTCT+GGG - chr8.1:26741277-26741296 None:intergenic 40.0%
!! GAGATTCTAGAATGTGGGAT+TGG + chr8.1:26741672-26741691 MS.gene011798:CDS 40.0%
!! TAGGGGAGATTCTAGAATGT+GGG + chr8.1:26741667-26741686 MS.gene011798:CDS 40.0%
!! TGGAAAATAGGAGAGTGTTG+TGG - chr8.1:26740905-26740924 None:intergenic 40.0%
!!! TGGTGGATTTGGTTTTGGTT+TGG - chr8.1:26740865-26740884 None:intergenic 40.0%
AAATAATCCTCGTCAAGCGG+AGG - chr8.1:26741609-26741628 None:intergenic 45.0%
CTCAACCACGTTGTTTGTGA+TGG + chr8.1:26741201-26741220 MS.gene011798:CDS 45.0%
GCCTGTTAAGGGTGGAAAAT+AGG - chr8.1:26740917-26740936 None:intergenic 45.0%
GGAAGGAATTGAAGATCCCA+TGG - chr8.1:26740799-26740818 None:intergenic 45.0%
GGGAAATGATGCTGAGTTCT+TGG + chr8.1:26742004-26742023 MS.gene011798:CDS 45.0%
GGGAACCAACGTTGAAATGA+AGG - chr8.1:26741297-26741316 None:intergenic 45.0%
GGGAAGATTACTGTGTTTGC+AGG + chr8.1:26741921-26741940 MS.gene011798:CDS 45.0%
GTTTGTGGCTACGTTGTGTT+GGG - chr8.1:26740953-26740972 None:intergenic 45.0%
TATCTTCGTCCACGCAAAAG+CGG + chr8.1:26741017-26741036 MS.gene011798:CDS 45.0%
TCTTGCCATCACAAACAACG+TGG - chr8.1:26741209-26741228 None:intergenic 45.0%
TGTTTGTGGCTACGTTGTGT+TGG - chr8.1:26740954-26740973 None:intergenic 45.0%
! CCGCTTGACGAGGATTATTT+TGG + chr8.1:26741609-26741628 MS.gene011798:CDS 45.0%
! CTTTCAGGTAAGTTCTGCGT+CGG - chr8.1:26741381-26741400 None:intergenic 45.0%
!! GTAGGGGAGATTCTAGAATG+TGG + chr8.1:26741666-26741685 MS.gene011798:CDS 45.0%
!! TTGTGTTTTCCGCTTTTGCG+TGG - chr8.1:26741029-26741048 None:intergenic 45.0%
!!! TGATTTTGGTTGGGGGAAAC+CGG + chr8.1:26741863-26741882 MS.gene011798:CDS 45.0%
ATAAGACGGCCTGTTAAGGG+TGG - chr8.1:26740925-26740944 None:intergenic 50.0%
GTGTTTGCAGGAGCTGAAGA+AGG + chr8.1:26741933-26741952 MS.gene011798:CDS 50.0%
TCAAGCGGAGGAATCATCCT+TGG - chr8.1:26741597-26741616 None:intergenic 50.0%
AAACCGGTAGGGGTTCGAAG+TGG + chr8.1:26741879-26741898 MS.gene011798:CDS 55.0%
AACCGGTAGGGGTTCGAAGT+GGG + chr8.1:26741880-26741899 MS.gene011798:CDS 55.0%
CGTGGACGAAGATAGCACCA+AGG - chr8.1:26741011-26741030 None:intergenic 55.0%
GATGATTCCTCCGCTTGACG+AGG + chr8.1:26741599-26741618 MS.gene011798:CDS 55.0%
TGGCTACGAGTAGTTCTCCG+AGG + chr8.1:26741823-26741842 MS.gene011798:CDS 55.0%
TTGGTTGGGGGAAACCGGTA+GGG + chr8.1:26741868-26741887 MS.gene011798:CDS 55.0%
TTTGGTTGGGGGAAACCGGT+AGG + chr8.1:26741867-26741886 MS.gene011798:CDS 55.0%
CTCCCACTTCGAACCCCTAC+CGG - chr8.1:26741885-26741904 None:intergenic 60.0%
TGGTTGGGGGAAACCGGTAG+GGG + chr8.1:26741869-26741888 MS.gene011798:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 26740697 26742043 26740697 ID=MS.gene011798
chr8.1 mRNA 26740697 26742043 26740697 ID=MS.gene011798.t1;Parent=MS.gene011798
chr8.1 exon 26740697 26742043 26740697 ID=MS.gene011798.t1.exon1;Parent=MS.gene011798.t1
chr8.1 CDS 26740697 26742043 26740697 ID=cds.MS.gene011798.t1;Parent=MS.gene011798.t1
Gene Sequence

>MS.gene011798

ATGGATTCCATCACAGTGATCTCAATTAGTATAATCCAAGCACCAATTGACAACAATAATCTCAACTCAAGAAGTGATTATCAGATAATCGATTTAACACCATGGGATCTTCAATTCCTTCCACTTGAAACCATCCAAAAAGGCCTTCTTTTCCATCAATCCATTCCAAACCAAAACCAAATCCACCATTTGAAACAAACACTTTCCACAACACTCTCCTATTTTCCACCCTTAACAGGCCGTCTTATAATCACCCAACACAACGTAGCCACAAACAACAACAACACATCATGTTCCATCATTTGCAACAACCTTGGTGCTATCTTCGTCCACGCAAAAGCGGAAAACACAACTGTATCAGACATCCTTAAACCAAACTATGTTCCTTCTATAGTTCACTCATTTTTCCCACTTAACGGAGTTAAAAACTACGAAGCCACTTCACAACCTATTCTAGCAGTCCAAGTAACGGAGTTAGCTAATGGCATCTTCATTGGTTTGACTCTCAACCACGTTGTTTGTGATGGCAAGATGTTTTGGCATTTCGTCAATACTTGGTCACAAATCTCAAAAAACTCCCAGAGTATACCAAAACTCCCTTCATTTCAACGTTGGTTCCCAAACGACGTTGAACTTCCAATACAATTTCCTTTCACAAAAACCGAATCACAATGTAGAAAACCGACGCAGAACTTACCTGAAAGAATTTTCCATTTTACAAAGGAGAAAATCGCGGAACTGAAATCAAAAGCAAATGAAGAAACCAAAACAGAGAATGTTCAAATCAAAATATCTTCGCTTCAAGCGTTGTTCTCTCATGTATGGCGATGTGTTGTTCGAAGCAAAAAACTCGATCCAGAAGAAGATTTTCATTACGTTTTAGTTATAGGTGTTAGACCAAGGATGATTCCTCCGCTTGACGAGGATTATTTTGGAAATGCAGGGATCATTGGTAGTGTAACTATGAAAGTAGGGGAGATTCTAGAATGTGGGATTGGAAAATGTTCATTGGAGATGAACAAGATGATTATGTCACATTCTGATGAGAAAATAAGGGATGATTATGAGTGTTGGTTGAGAATGCCGAAGTTGTTTGGGATTAATAGTTTGGCAGGTGCTAATTCATTGGCTACGAGTAGTTCTCCGAGGTTTGATGTTTATGGTAATGATTTTGGTTGGGGGAAACCGGTAGGGGTTCGAAGTGGGAGTGCAAATAAAAGTAATGGGAAGATTACTGTGTTTGCAGGAGCTGAAGAAGGTAGCATTGATATTGAAGTTTGTCTTTCTTTTGAGATATTGGAGGCTTTGGGAAATGATGCTGAGTTCTTGGTTCATAGTTCTAAGTAA
Protein sequence

>MS.gene011798.t1

MDSITVISISIIQAPIDNNNLNSRSDYQIIDLTPWDLQFLPLETIQKGLLFHQSIPNQNQIHHLKQTLSTTLSYFPPLTGRLIITQHNVATNNNNTSCSIICNNLGAIFVHAKAENTTVSDILKPNYVPSIVHSFFPLNGVKNYEATSQPILAVQVTELANGIFIGLTLNHVVCDGKMFWHFVNTWSQISKNSQSIPKLPSFQRWFPNDVELPIQFPFTKTESQCRKPTQNLPERIFHFTKEKIAELKSKANEETKTENVQIKISSLQALFSHVWRCVVRSKKLDPEEDFHYVLVIGVRPRMIPPLDEDYFGNAGIIGSVTMKVGEILECGIGKCSLEMNKMIMSHSDEKIRDDYECWLRMPKLFGINSLAGANSLATSSSPRFDVYGNDFGWGKPVGVRSGSANKSNGKITVFAGAEEGSIDIEVCLSFEILEALGNDAEFLVHSSK