AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066701

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066701.t1 MTR_4g022290 86.830 448 53 2 1 447 1 443 0.0 784
MS.gene066701.t1 MTR_4g094828 82.857 455 61 3 1 446 1 447 0.0 766
MS.gene066701.t1 MTR_8g079710 75.166 451 100 7 1 447 1 443 0.0 669
MS.gene066701.t1 MTR_4g094835 54.386 456 183 10 1 445 1 442 1.30e-166 477
MS.gene066701.t1 MTR_4g094842 52.941 459 181 8 1 442 1 441 1.85e-159 459
MS.gene066701.t1 MTR_0190s0020 49.890 455 203 6 1 446 1 439 2.35e-151 438
MS.gene066701.t1 MTR_4g022800 50.862 464 185 11 1 446 1 439 9.32e-148 429
MS.gene066701.t1 MTR_3g026310 50.000 476 187 13 1 452 1 449 4.35e-147 427
MS.gene066701.t1 MTR_4g094838 51.448 449 178 10 6 441 3 424 4.67e-147 426
MS.gene066701.t1 MTR_4g022825 50.429 466 182 12 1 446 1 437 1.09e-146 426
MS.gene066701.t1 MTR_4g022720 50.766 457 189 11 1 446 1 432 2.07e-145 422
MS.gene066701.t1 MTR_3g088420 50.000 458 189 12 1 446 1 430 6.37e-144 419
MS.gene066701.t1 MTR_4g022670 49.676 463 191 11 1 446 1 438 7.47e-144 419
MS.gene066701.t1 MTR_7g026860 50.000 462 188 11 1 446 1 435 1.88e-143 417
MS.gene066701.t1 MTR_8g041770 49.342 456 203 10 1 446 1 438 3.18e-143 417
MS.gene066701.t1 MTR_4g094832 55.381 381 143 8 73 441 57 422 5.41e-138 403
MS.gene066701.t1 MTR_4g022760 44.589 462 172 10 1 446 1 394 4.22e-118 351
MS.gene066701.t1 MTR_6g016815 40.618 453 175 10 1 447 15 379 1.90e-98 300
MS.gene066701.t1 MTR_3g101900 34.755 469 263 12 6 453 1 447 7.56e-82 260
MS.gene066701.t1 MTR_3g101970 37.209 430 218 14 21 430 21 418 5.25e-80 255
MS.gene066701.t1 MTR_3g101960 36.364 440 243 11 21 441 21 442 8.42e-80 255
MS.gene066701.t1 MTR_3g102010 36.222 450 252 12 21 452 22 454 9.23e-80 254
MS.gene066701.t1 MTR_3g101930 35.590 458 247 14 21 454 22 455 5.43e-79 253
MS.gene066701.t1 MTR_3g102020 35.714 448 250 12 21 448 21 450 2.20e-78 251
MS.gene066701.t1 MTR_8g040760 55.760 217 88 4 1 212 1 214 6.89e-77 239
MS.gene066701.t1 MTR_5g081960 32.665 499 258 14 6 450 8 482 1.98e-66 221
MS.gene066701.t1 MTR_4g094825 56.915 188 75 3 20 202 83 269 8.21e-63 209
MS.gene066701.t1 MTR_4g094825 47.945 146 62 4 297 441 251 383 2.45e-33 130
MS.gene066701.t1 MTR_3g070170 32.527 455 260 11 19 441 23 462 4.03e-62 209
MS.gene066701.t1 MTR_4g015180 33.197 488 260 14 8 441 4 479 4.87e-62 209
MS.gene066701.t1 MTR_7g026850 61.392 158 58 3 28 184 7 162 8.57e-61 196
MS.gene066701.t1 MTR_8g040780 40.833 240 123 5 206 442 2 225 2.72e-47 163
MS.gene066701.t1 MTR_6g034495 66.379 116 37 1 70 183 45 160 1.22e-46 159
MS.gene066701.t1 MTR_3g102030 32.174 345 181 13 70 402 17 320 1.28e-42 153
MS.gene066701.t1 MTR_6g045553 27.115 461 280 9 19 441 25 467 4.29e-38 144
MS.gene066701.t1 MTR_7g026770 42.781 187 78 4 146 332 132 289 2.29e-36 136
MS.gene066701.t1 MTR_7g026830 70.732 82 24 0 89 170 16 97 7.15e-35 125
MS.gene066701.t1 MTR_8g075610 26.065 399 244 15 59 438 47 413 8.14e-26 109
MS.gene066701.t1 MTR_6g034485 40.000 140 65 3 191 326 1 125 9.89e-26 102
MS.gene066701.t1 MTR_2g105330 26.087 414 248 18 46 433 39 420 4.53e-24 104
MS.gene066701.t1 MTR_4g007540 25.304 411 256 17 46 433 39 421 6.42e-24 103
MS.gene066701.t1 MTR_2g064495 26.087 414 248 18 46 433 39 420 6.42e-24 103
MS.gene066701.t1 MTR_3g026950 26.096 456 266 21 19 434 13 437 3.86e-22 99.0
MS.gene066701.t1 MTR_3g049150 24.651 430 249 16 46 434 38 433 4.09e-21 95.9
MS.gene066701.t1 MTR_8g096530 25.634 355 217 13 59 397 59 382 8.32e-20 92.0
MS.gene066701.t1 MTR_1g040195 24.194 434 282 14 30 453 23 419 1.25e-19 91.3
MS.gene066701.t1 MTR_7g014200 24.946 461 265 18 27 436 28 458 1.99e-19 90.9
MS.gene066701.t1 MTR_1g040175 24.397 373 243 14 34 398 46 387 3.95e-19 90.1
MS.gene066701.t1 MTR_1g081660 23.516 455 264 18 29 441 31 443 5.42e-19 89.4
MS.gene066701.t1 MTR_4g097090 23.608 449 258 20 29 430 30 440 5.87e-19 89.4
MS.gene066701.t1 MTR_5g076700 24.541 436 263 18 30 431 24 427 1.95e-18 87.8
MS.gene066701.t1 MTR_7g114470 23.341 437 268 18 30 431 25 429 2.28e-18 87.8
MS.gene066701.t1 MTR_8g012750 25.768 423 240 18 26 397 23 422 3.31e-18 87.4
MS.gene066701.t1 MTR_2g089765 23.605 466 260 21 26 430 36 466 4.75e-18 86.7
MS.gene066701.t1 MTR_3g098980 23.038 395 253 13 59 436 66 426 5.16e-18 86.3
MS.gene066701.t1 MTR_7g014330 24.524 420 279 14 29 422 26 433 1.00e-17 85.5
MS.gene066701.t1 MTR_7g027175 21.939 392 280 11 49 435 58 428 1.90e-17 84.7
MS.gene066701.t1 MTR_4g013050 24.010 404 217 16 57 430 51 394 2.97e-17 84.0
MS.gene066701.t1 MTR_3g087640 24.161 447 268 18 34 430 31 456 3.98e-17 84.0
MS.gene066701.t1 MTR_7g086000 26.196 439 239 22 59 448 60 462 4.09e-17 84.0
MS.gene066701.t1 MTR_6g015830 24.700 417 234 14 29 400 34 415 9.73e-17 82.8
MS.gene066701.t1 MTR_3g028210 23.476 443 285 15 29 436 31 454 1.37e-16 82.4
MS.gene066701.t1 MTR_6g015320 24.623 398 257 15 29 400 29 409 6.03e-16 80.1
MS.gene066701.t1 MTR_7g014250 23.557 433 259 17 33 422 30 433 7.06e-16 80.1
MS.gene066701.t1 MTR_4g127650 23.261 417 249 17 59 438 66 448 8.12e-16 79.7
MS.gene066701.t1 MTR_2g089670 24.619 394 238 17 41 397 58 429 1.52e-15 79.0
MS.gene066701.t1 MTR_7g014610 23.340 467 281 17 27 440 27 469 2.12e-15 78.6
MS.gene066701.t1 MTR_7g084940 27.654 358 210 16 57 397 67 392 3.07e-15 77.8
MS.gene066701.t1 MTR_5g084870 25.000 448 271 19 3 427 2 407 4.23e-15 77.4
MS.gene066701.t1 MTR_3g071320 26.471 340 189 15 26 334 23 332 5.08e-15 77.4
MS.gene066701.t1 MTR_7g114490 23.356 441 269 19 30 435 24 430 5.92e-15 77.4
MS.gene066701.t1 MTR_1g040320 23.245 413 241 15 54 453 59 408 1.43e-14 75.9
MS.gene066701.t1 MTR_5g090810 22.927 410 235 14 37 398 36 412 2.02e-14 75.5
MS.gene066701.t1 MTR_3g086020 26.905 420 245 20 28 428 22 398 2.31e-14 75.1
MS.gene066701.t1 MTR_6g015850 24.155 414 237 13 29 400 34 412 2.57e-14 75.1
MS.gene066701.t1 MTR_5g090880 24.409 381 214 16 59 398 67 414 2.63e-14 75.1
MS.gene066701.t1 MTR_4g055170 25.517 290 187 9 59 337 69 340 2.78e-14 75.1
MS.gene066701.t1 MTR_3g112180 26.645 304 173 11 33 315 38 312 7.55e-14 73.6
MS.gene066701.t1 MTR_6g053810 25.852 352 223 15 59 393 71 401 8.34e-14 73.6
MS.gene066701.t1 MTR_5g090830 22.683 410 236 14 37 398 36 412 1.09e-13 73.2
MS.gene066701.t1 MTR_8g079950 25.835 449 259 21 3 422 2 405 1.49e-13 72.8
MS.gene066701.t1 MTR_2g089755 25.428 409 257 17 29 401 37 433 2.69e-13 72.0
MS.gene066701.t1 MTR_8g079980 22.021 386 253 14 52 422 52 404 1.98e-12 69.3
MS.gene066701.t1 MTR_7g013850 22.222 459 283 17 32 436 32 470 2.57e-12 68.9
MS.gene066701.t1 MTR_7g110610 29.126 206 131 8 49 252 47 239 5.90e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066701.t1 AT5G67150 37.716 464 254 13 3 453 4 445 6.40e-96 296
MS.gene066701.t1 AT3G50300 37.901 467 248 15 1 447 1 445 3.96e-86 271
MS.gene066701.t1 AT3G50270 36.607 448 249 12 4 435 6 434 1.84e-85 269
MS.gene066701.t1 AT5G67160 36.748 449 251 10 1 441 1 424 1.42e-84 266
MS.gene066701.t1 AT5G38130 37.585 439 248 12 1 430 21 442 8.79e-83 263
MS.gene066701.t1 AT3G50280 35.635 449 255 12 1 435 1 429 1.53e-81 259
MS.gene066701.t1 AT5G42830 35.116 430 246 11 28 441 29 441 7.57e-77 247
MS.gene066701.t1 AT5G07850 34.491 432 249 9 28 441 30 445 3.60e-73 238
MS.gene066701.t1 AT5G07860 35.035 431 244 11 28 441 30 441 8.48e-71 231
MS.gene066701.t1 AT2G39980 32.903 465 259 10 19 441 19 472 1.16e-68 226
MS.gene066701.t1 AT5G01210 33.624 458 264 11 19 445 17 465 4.21e-67 222
MS.gene066701.t1 AT5G07870 34.222 450 250 12 28 448 30 462 6.68e-65 216
MS.gene066701.t1 AT3G50280 35.032 314 181 7 130 435 1 299 3.11e-53 181
MS.gene066701.t1 AT3G50290 37.762 286 147 7 124 395 1 269 8.38e-50 171
MS.gene066701.t1 AT5G23940 30.995 442 256 12 25 438 21 441 6.79e-49 174
MS.gene066701.t1 AT5G41040 27.708 397 239 17 59 438 85 450 2.03e-24 105
MS.gene066701.t1 AT5G41040 27.708 397 239 17 59 438 69 434 2.06e-24 105
MS.gene066701.t1 AT5G48930 25.428 409 252 16 46 430 39 418 1.16e-22 100
MS.gene066701.t1 AT3G29590 23.529 442 281 15 29 440 28 442 1.38e-21 97.4
MS.gene066701.t1 AT3G48720 26.392 413 254 18 44 437 42 423 3.89e-21 95.9
MS.gene066701.t1 AT5G63560 25.316 395 231 16 59 431 61 413 4.06e-20 92.8
MS.gene066701.t1 AT1G24430 25.917 409 245 17 34 422 29 399 3.08e-19 90.1
MS.gene066701.t1 AT2G19070 25.000 460 280 18 21 438 7 443 4.53e-19 89.7
MS.gene066701.t1 AT5G57840 25.238 420 243 18 46 430 39 422 2.37e-18 87.4
MS.gene066701.t1 AT5G07080 25.145 346 200 11 59 377 70 383 2.27e-17 84.7
MS.gene066701.t1 AT3G03480 24.661 442 263 18 30 436 33 439 3.00e-17 84.3
MS.gene066701.t1 AT1G78990 26.471 374 219 14 48 398 56 396 3.71e-17 84.0
MS.gene066701.t1 AT5G47950 24.235 392 241 14 54 433 56 403 7.56e-17 82.8
MS.gene066701.t1 AT5G17540 24.270 445 252 18 30 431 23 425 1.83e-16 82.0
MS.gene066701.t1 AT1G32910 24.661 369 215 13 59 398 65 399 6.04e-16 80.5
MS.gene066701.t1 AT5G39080 23.758 463 295 16 13 438 12 453 9.77e-16 79.7
MS.gene066701.t1 AT3G26040 27.465 426 233 20 28 420 20 402 1.83e-15 78.6
MS.gene066701.t1 AT3G30280 23.469 392 248 18 58 431 61 418 6.91e-15 77.0
MS.gene066701.t1 AT5G16410 23.558 416 240 17 46 424 77 451 2.65e-14 75.5
MS.gene066701.t1 AT5G23970 23.844 411 244 16 45 431 38 403 2.79e-14 75.1
MS.gene066701.t1 AT1G24420 24.658 365 226 14 70 422 76 403 7.79e-14 73.6
MS.gene066701.t1 AT1G24420 24.658 365 226 14 70 422 76 403 7.79e-14 73.6
MS.gene066701.t1 AT2G40230 23.919 393 267 13 20 400 17 389 3.79e-13 71.6
MS.gene066701.t1 AT4G15390 22.059 408 255 15 59 442 64 432 7.55e-12 67.4
MS.gene066701.t1 AT4G15390 22.059 408 255 15 59 442 64 432 7.55e-12 67.4
MS.gene066701.t1 AT5G47980 24.939 413 237 19 58 441 60 428 3.45e-11 65.5
MS.gene066701.t1 AT5G39090 23.195 457 275 18 13 430 12 431 4.14e-11 65.1
MS.gene066701.t1 AT1G27620 22.829 403 249 15 59 438 58 421 5.86e-11 64.7
MS.gene066701.t1 AT1G31490 24.879 414 231 20 18 398 18 384 9.34e-11 63.9

Find 101 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTTCATCAATAATGTTAT+TGG 0.138724 4.3:+76291953 MS.gene066701:CDS
TTGTCTTCCTTATGATATTT+TGG 0.184663 4.3:+76291895 MS.gene066701:CDS
AAGTTAGAAGAGAATTATTT+TGG 0.198109 4.3:+76291524 MS.gene066701:CDS
ATGGTCTATTTGGTTTGTTT+TGG 0.227446 4.3:-76290763 None:intergenic
AGGAACTATTGTTGGCTTAT+TGG 0.232541 4.3:-76291165 None:intergenic
CATTCTTGTCCTACAATCTA+TGG 0.234125 4.3:-76291495 None:intergenic
GGATCTCAAATTTCTCAAAT+TGG 0.239669 4.3:+76290707 MS.gene066701:CDS
GGATATGTAATAATGCAAAA+AGG 0.251454 4.3:+76291566 MS.gene066701:CDS
TTTGTTTAAGATGGTCTATT+TGG 0.294119 4.3:-76290773 None:intergenic
CATCACCTTGATTTGTTGCT+TGG 0.302101 4.3:-76290647 None:intergenic
TATGGAAAAGAAGACCTCTT+TGG 0.321294 4.3:-76290737 None:intergenic
ATGGGACGGTTAACATTGTT+TGG 0.329441 4.3:-76291206 None:intergenic
TGAGATCCCATGGAGATAAA+TGG 0.346302 4.3:-76290692 None:intergenic
AAATAATTCTCTTCTAACTT+TGG 0.349532 4.3:-76291521 None:intergenic
GTGTAGACGGCAAGTCATTT+TGG 0.349934 4.3:+76291105 MS.gene066701:CDS
ATGGAAAAGAAGACCTCTTT+GGG 0.360598 4.3:-76290736 None:intergenic
ACGAATGCAGACCTTTCTTC+TGG 0.360777 4.3:-76291467 None:intergenic
GTTGAAAACCCCGTTTATAT+CGG 0.362533 4.3:+76291691 MS.gene066701:CDS
TTTGGCATTTCATCAACTCT+TGG 0.368556 4.3:+76291123 MS.gene066701:CDS
TTACGGTAATGACTTTGGTT+GGG 0.373170 4.3:+76291772 MS.gene066701:CDS
TTGGCATTTCATCAACTCTT+GGG 0.376104 4.3:+76291124 MS.gene066701:CDS
ATGGAACATGAGGCGTTGTT+AGG 0.379377 4.3:-76290864 None:intergenic
AGCCTATTGCAGTTAGAAAT+GGG 0.388093 4.3:+76291798 MS.gene066701:CDS
GTTGGACTGCAAAGAAATAT+TGG 0.392396 4.3:+76291821 MS.gene066701:CDS
ACCCCATTTCTAACTGCAAT+AGG 0.394232 4.3:-76291800 None:intergenic
TCGTCTTACCCATAGATTGT+AGG 0.400779 4.3:+76291486 MS.gene066701:CDS
AAGCCTATTGCAGTTAGAAA+TGG 0.401535 4.3:+76291797 MS.gene066701:CDS
AACTATTACGGTAATGACTT+TGG 0.405935 4.3:+76291767 MS.gene066701:CDS
TTAATCGACGGTATCTTCAT+TGG 0.408333 4.3:+76291062 MS.gene066701:CDS
TCGAAAGAAGGAACTATTGT+TGG 0.415115 4.3:-76291173 None:intergenic
AGGAAAGTGTTTGTTTAAGA+TGG 0.419599 4.3:-76290782 None:intergenic
TCACCACGGTTCAACTATTA+CGG 0.422782 4.3:+76291755 MS.gene066701:CDS
CTACCTGGCTCCGATATAAA+CGG 0.425817 4.3:-76291701 None:intergenic
TACCTGGCTCCGATATAAAC+GGG 0.427106 4.3:-76291700 None:intergenic
GGGAGTGGAATCGGAGCAAC+TGG 0.428061 4.3:+76291545 MS.gene066701:CDS
AAATTATCCATTTATCTCCA+TGG 0.447952 4.3:+76290685 MS.gene066701:CDS
ATTACGGTAATGACTTTGGT+TGG 0.447960 4.3:+76291771 MS.gene066701:CDS
TACGGTAATGACTTTGGTTG+GGG 0.461686 4.3:+76291773 MS.gene066701:CDS
GGAAACCAGCGTTCGAAAGA+AGG 0.465595 4.3:-76291185 None:intergenic
AAAGGGGAATTAATGGAAAG+TGG 0.488381 4.3:+76291584 MS.gene066701:CDS
GTGAATGGTGGGGCAGGAGA+AGG 0.488559 4.3:+76291854 MS.gene066701:CDS
TAAGAATAATGAAGAAACAA+TGG 0.493631 4.3:-76290976 None:intergenic
ATAGCATCCAAAATATCATA+AGG 0.495132 4.3:-76291902 None:intergenic
ACAATGGGAGGAACATACTT+TGG 0.495798 4.3:-76290960 None:intergenic
AAGAATAATGAAGAAACAAT+GGG 0.496513 4.3:-76290975 None:intergenic
GTTATTGTGAATGGTGGGGC+AGG 0.497656 4.3:+76291848 MS.gene066701:CDS
TTGAGAAATTTGAGATCCCA+TGG 0.499411 4.3:-76290702 None:intergenic
GGTAGAGTTATTGTGAATGG+TGG 0.501616 4.3:+76291842 MS.gene066701:CDS
GAATTAATGGAAAGTGGGAT+TGG 0.504651 4.3:+76291590 MS.gene066701:CDS
ACCAGCGTTGTTACAAATGA+TGG 0.505482 4.3:-76290883 None:intergenic
AATTATCCATTTATCTCCAT+GGG 0.510570 4.3:+76290686 MS.gene066701:CDS
ACAATCCAAGCAACAAATCA+AGG 0.513131 4.3:+76290642 MS.gene066701:CDS
ATTGGTAGAGTTATTGTGAA+TGG 0.514289 4.3:+76291839 MS.gene066701:CDS
TGGGATTGGTAAAATTGCTA+TGG 0.515611 4.3:+76291604 MS.gene066701:CDS
AACTTCTTTCCACCACTCGC+TGG 0.516081 4.3:+76290813 MS.gene066701:CDS
AACTAGAATCCATGGACATA+GGG 0.518441 4.3:-76291934 None:intergenic
TAATGAGTGATATTGAATCT+TGG 0.525795 4.3:+76291669 MS.gene066701:CDS
TACTTATAATTAAACGCCAA+AGG 0.529613 4.3:-76291430 None:intergenic
AAGGGGAATTAATGGAAAGT+GGG 0.533012 4.3:+76291585 MS.gene066701:CDS
AAACTAGAATCCATGGACAT+AGG 0.534361 4.3:-76291935 None:intergenic
AAAATCTTAAACCAGAAGAA+AGG 0.538946 4.3:+76291456 MS.gene066701:CDS
CATACGGTTTCCTTTCACAA+AGG 0.546568 4.3:+76291226 MS.gene066701:CDS
ACCCCGTTTATATCGGAGCC+AGG 0.547815 4.3:+76291698 MS.gene066701:CDS
ATTGATGAAACTAGAATCCA+TGG 0.549335 4.3:-76291942 None:intergenic
AGATTACTTGATGATCTACC+TGG 0.550288 4.3:-76291716 None:intergenic
AGGAAACGACCCTATGTCCA+TGG 0.554536 4.3:+76291925 MS.gene066701:CDS
ATCAAAATCTCATCATGAGA+AGG 0.558207 4.3:+76291646 MS.gene066701:CDS
TCGAATCTTTCACTTTACAA+AGG 0.560649 4.3:+76291283 MS.gene066701:CDS
TGGTTGAAGTATGTCGGCAA+TGG 0.566982 4.3:-76290940 None:intergenic
ATTGCAGTTAGAAATGGGGT+TGG 0.568648 4.3:+76291803 MS.gene066701:CDS
ATACTTTGGTTGAAGTATGT+CGG 0.572459 4.3:-76290946 None:intergenic
AAAAGAAGACCTCTTTGGGT+TGG 0.572933 4.3:-76290732 None:intergenic
ATTCTTGTCCTACAATCTAT+GGG 0.578627 4.3:-76291494 None:intergenic
CTTTGTGAAAGGAAACCGTA+TGG 0.579781 4.3:-76291225 None:intergenic
TAGTTCCTTCTTTCGAACGC+TGG 0.581888 4.3:+76291180 MS.gene066701:CDS
GTAGAGTTATTGTGAATGGT+GGG 0.583851 4.3:+76291843 MS.gene066701:CDS
ACAATGTTAACCGTCCCATA+CGG 0.591996 4.3:+76291210 MS.gene066701:CDS
TGGTGGAAAGAAGTTAAGTG+TGG 0.592664 4.3:-76290805 None:intergenic
GTTACTTGTACTGCAAGTAG+TGG 0.602193 4.3:-76291035 None:intergenic
ATTAACCATGTGTGTGTAGA+CGG 0.608212 4.3:+76291092 MS.gene066701:CDS
GCCTATTGCAGTTAGAAATG+GGG 0.609427 4.3:+76291799 MS.gene066701:CDS
TCCATCATTTGTAACAACGC+TGG 0.618527 4.3:+76290882 MS.gene066701:CDS
GATAAGTAGAATCTCTGTCA+TGG 0.620107 4.3:-76290610 None:intergenic
GCTAATGCAGAAATTAGTAG+TGG 0.623588 4.3:+76291332 MS.gene066701:CDS
ACAAGACGACCAGCGAGTGG+TGG 0.630223 4.3:-76290822 None:intergenic
TGAAAGGAAACCGTATGGGA+CGG 0.630636 4.3:-76291220 None:intergenic
GTTACAAATGATGGAACATG+AGG 0.636643 4.3:-76290874 None:intergenic
AATTGCTATGGAAATGAACA+AGG 0.638167 4.3:+76291616 MS.gene066701:CDS
AAATTGGCACCAACCCAAAG+AGG 0.639346 4.3:+76290723 MS.gene066701:CDS
TTTGTGAAAGGAAACCGTAT+GGG 0.644706 4.3:-76291224 None:intergenic
TCTTGGGCTGAAATCTCACA+AGG 0.661149 4.3:+76291140 MS.gene066701:CDS
TGGAAAGAAGTTAAGTGTGG+AGG 0.665000 4.3:-76290802 None:intergenic
CAAGTAACAGAGTTAATCGA+CGG 0.676652 4.3:+76291050 MS.gene066701:CDS
AATAATGAAGAAACAATGGG+AGG 0.681867 4.3:-76290972 None:intergenic
ACTTGCCGTCTACACACACA+TGG 0.683354 4.3:-76291097 None:intergenic
ATTACAAGACGACCAGCGAG+TGG 0.695572 4.3:-76290825 None:intergenic
TGATAGCAAGCAGTTCACCA+CGG 0.696229 4.3:+76291741 MS.gene066701:CDS
AATGATGTGAAAAGCTACGA+AGG 0.699069 4.3:+76291002 MS.gene066701:CDS
TAGAGTTATTGTGAATGGTG+GGG 0.705324 4.3:+76291844 MS.gene066701:CDS
ACCTGGCTCCGATATAAACG+GGG 0.710991 4.3:-76291699 None:intergenic
TTACCGTAATAGTTGAACCG+TGG 0.734430 4.3:-76291758 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTCTCTTCTAACTT+TGG - chr4.3:76291524-76291543 None:intergenic 20.0%
!! AAGAATAATGAAGAAACAAT+GGG - chr4.3:76290978-76290997 None:intergenic 20.0%
!! AGTTTCATCAATAATGTTAT+TGG + chr4.3:76291953-76291972 MS.gene066701:CDS 20.0%
!! ATATGTAATAATGCAAAAAG+GGG + chr4.3:76291568-76291587 MS.gene066701:CDS 20.0%
!! GATATGTAATAATGCAAAAA+GGG + chr4.3:76291567-76291586 MS.gene066701:CDS 20.0%
!! TAAGAATAATGAAGAAACAA+TGG - chr4.3:76290979-76290998 None:intergenic 20.0%
!!! AAGTTAGAAGAGAATTATTT+TGG + chr4.3:76291524-76291543 MS.gene066701:CDS 20.0%
!!! AGTTAGAAGAGAATTATTTT+GGG + chr4.3:76291525-76291544 MS.gene066701:CDS 20.0%
! AAAATCTTAAACCAGAAGAA+AGG + chr4.3:76291456-76291475 MS.gene066701:CDS 25.0%
! AAATTATCCATTTATCTCCA+TGG + chr4.3:76290685-76290704 MS.gene066701:CDS 25.0%
! AATTATCCATTTATCTCCAT+GGG + chr4.3:76290686-76290705 MS.gene066701:CDS 25.0%
! ATAGCATCCAAAATATCATA+AGG - chr4.3:76291905-76291924 None:intergenic 25.0%
! GGATATGTAATAATGCAAAA+AGG + chr4.3:76291566-76291585 MS.gene066701:CDS 25.0%
! TAATGAGTGATATTGAATCT+TGG + chr4.3:76291669-76291688 MS.gene066701:CDS 25.0%
! TACTTATAATTAAACGCCAA+AGG - chr4.3:76291433-76291452 None:intergenic 25.0%
!! TTGTCTTCCTTATGATATTT+TGG + chr4.3:76291895-76291914 MS.gene066701:CDS 25.0%
!! TTTGTTTAAGATGGTCTATT+TGG - chr4.3:76290776-76290795 None:intergenic 25.0%
!!! TATGATATTTTGGATGCTAT+AGG + chr4.3:76291905-76291924 MS.gene066701:CDS 25.0%
AACTATTACGGTAATGACTT+TGG + chr4.3:76291767-76291786 MS.gene066701:CDS 30.0%
AATAATGAAGAAACAATGGG+AGG - chr4.3:76290975-76290994 None:intergenic 30.0%
AATGCAAAAAGGGGAATTAA+TGG + chr4.3:76291577-76291596 MS.gene066701:CDS 30.0%
AATTGCTATGGAAATGAACA+AGG + chr4.3:76291616-76291635 MS.gene066701:CDS 30.0%
ATACTTTGGTTGAAGTATGT+CGG - chr4.3:76290949-76290968 None:intergenic 30.0%
ATCAAAATCTCATCATGAGA+AGG + chr4.3:76291646-76291665 MS.gene066701:CDS 30.0%
ATTCTTGTCCTACAATCTAT+GGG - chr4.3:76291497-76291516 None:intergenic 30.0%
ATTGATGAAACTAGAATCCA+TGG - chr4.3:76291945-76291964 None:intergenic 30.0%
ATTGGTAGAGTTATTGTGAA+TGG + chr4.3:76291839-76291858 MS.gene066701:CDS 30.0%
GGATCTCAAATTTCTCAAAT+TGG + chr4.3:76290707-76290726 MS.gene066701:CDS 30.0%
TCGAATCTTTCACTTTACAA+AGG + chr4.3:76291283-76291302 MS.gene066701:CDS 30.0%
!! AGGAAAGTGTTTGTTTAAGA+TGG - chr4.3:76290785-76290804 None:intergenic 30.0%
!!! AATTATTTTGGGAGTGGAAT+CGG + chr4.3:76291536-76291555 MS.gene066701:CDS 30.0%
!!! ATGGTCTATTTGGTTTGTTT+TGG - chr4.3:76290766-76290785 None:intergenic 30.0%
!!! TGGTCTATTTGGTTTGTTTT+GGG - chr4.3:76290765-76290784 None:intergenic 30.0%
!!! TTTGGTTTGTTTTGGGTTTA+TGG - chr4.3:76290758-76290777 None:intergenic 30.0%
AAACTAGAATCCATGGACAT+AGG - chr4.3:76291938-76291957 None:intergenic 35.0%
AAAGGGGAATTAATGGAAAG+TGG + chr4.3:76291584-76291603 MS.gene066701:CDS 35.0%
AACTAGAATCCATGGACATA+GGG - chr4.3:76291937-76291956 None:intergenic 35.0%
AAGCCTATTGCAGTTAGAAA+TGG + chr4.3:76291797-76291816 MS.gene066701:CDS 35.0%
AAGGGGAATTAATGGAAAGT+GGG + chr4.3:76291585-76291604 MS.gene066701:CDS 35.0%
AATGATGTGAAAAGCTACGA+AGG + chr4.3:76291002-76291021 MS.gene066701:CDS 35.0%
ACAATCCAAGCAACAAATCA+AGG + chr4.3:76290642-76290661 MS.gene066701:CDS 35.0%
AGCCTATTGCAGTTAGAAAT+GGG + chr4.3:76291798-76291817 MS.gene066701:CDS 35.0%
ATGGAAAAGAAGACCTCTTT+GGG - chr4.3:76290739-76290758 None:intergenic 35.0%
ATTAACCATGTGTGTGTAGA+CGG + chr4.3:76291092-76291111 MS.gene066701:CDS 35.0%
ATTACGGTAATGACTTTGGT+TGG + chr4.3:76291771-76291790 MS.gene066701:CDS 35.0%
CAAGTAACAGAGTTAATCGA+CGG + chr4.3:76291050-76291069 MS.gene066701:CDS 35.0%
CATTCTTGTCCTACAATCTA+TGG - chr4.3:76291498-76291517 None:intergenic 35.0%
GAATTAATGGAAAGTGGGAT+TGG + chr4.3:76291590-76291609 MS.gene066701:CDS 35.0%
GCTAATGCAGAAATTAGTAG+TGG + chr4.3:76291332-76291351 MS.gene066701:CDS 35.0%
GTAGAGTTATTGTGAATGGT+GGG + chr4.3:76291843-76291862 MS.gene066701:CDS 35.0%
GTTGAAAACCCCGTTTATAT+CGG + chr4.3:76291691-76291710 MS.gene066701:CDS 35.0%
GTTGGACTGCAAAGAAATAT+TGG + chr4.3:76291821-76291840 MS.gene066701:CDS 35.0%
TAGAGTTATTGTGAATGGTG+GGG + chr4.3:76291844-76291863 MS.gene066701:CDS 35.0%
TATGGAAAAGAAGACCTCTT+TGG - chr4.3:76290740-76290759 None:intergenic 35.0%
TCGAAAGAAGGAACTATTGT+TGG - chr4.3:76291176-76291195 None:intergenic 35.0%
TTAATCGACGGTATCTTCAT+TGG + chr4.3:76291062-76291081 MS.gene066701:CDS 35.0%
TTACGGTAATGACTTTGGTT+GGG + chr4.3:76291772-76291791 MS.gene066701:CDS 35.0%
TTGGCATTTCATCAACTCTT+GGG + chr4.3:76291124-76291143 MS.gene066701:CDS 35.0%
TTTGGCATTTCATCAACTCT+TGG + chr4.3:76291123-76291142 MS.gene066701:CDS 35.0%
TTTGTGAAAGGAAACCGTAT+GGG - chr4.3:76291227-76291246 None:intergenic 35.0%
! AGATTACTTGATGATCTACC+TGG - chr4.3:76291719-76291738 None:intergenic 35.0%
! GTTACAAATGATGGAACATG+AGG - chr4.3:76290877-76290896 None:intergenic 35.0%
! TGGGATTGGTAAAATTGCTA+TGG + chr4.3:76291604-76291623 MS.gene066701:CDS 35.0%
! TTGAGAAATTTGAGATCCCA+TGG - chr4.3:76290705-76290724 None:intergenic 35.0%
! TTTTGCACATCCTTTGTGAA+AGG - chr4.3:76291239-76291258 None:intergenic 35.0%
!! AGGAACTATTGTTGGCTTAT+TGG - chr4.3:76291168-76291187 None:intergenic 35.0%
!!! GAAGAGAATTATTTTGGGAG+TGG + chr4.3:76291530-76291549 MS.gene066701:CDS 35.0%
AAAAGAAGACCTCTTTGGGT+TGG - chr4.3:76290735-76290754 None:intergenic 40.0%
ACAATGGGAGGAACATACTT+TGG - chr4.3:76290963-76290982 None:intergenic 40.0%
ACAATGTTAACCGTCCCATA+CGG + chr4.3:76291210-76291229 MS.gene066701:CDS 40.0%
ACCAGCGTTGTTACAAATGA+TGG - chr4.3:76290886-76290905 None:intergenic 40.0%
ACCCCATTTCTAACTGCAAT+AGG - chr4.3:76291803-76291822 None:intergenic 40.0%
ATGGGACGGTTAACATTGTT+TGG - chr4.3:76291209-76291228 None:intergenic 40.0%
ATTGCAGTTAGAAATGGGGT+TGG + chr4.3:76291803-76291822 MS.gene066701:CDS 40.0%
CATACGGTTTCCTTTCACAA+AGG + chr4.3:76291226-76291245 MS.gene066701:CDS 40.0%
CTTTGTGAAAGGAAACCGTA+TGG - chr4.3:76291228-76291247 None:intergenic 40.0%
GCCTATTGCAGTTAGAAATG+GGG + chr4.3:76291799-76291818 MS.gene066701:CDS 40.0%
GGTAGAGTTATTGTGAATGG+TGG + chr4.3:76291842-76291861 MS.gene066701:CDS 40.0%
GTTACTTGTACTGCAAGTAG+TGG - chr4.3:76291038-76291057 None:intergenic 40.0%
TACGGTAATGACTTTGGTTG+GGG + chr4.3:76291773-76291792 MS.gene066701:CDS 40.0%
TCACCACGGTTCAACTATTA+CGG + chr4.3:76291755-76291774 MS.gene066701:CDS 40.0%
TCCATCATTTGTAACAACGC+TGG + chr4.3:76290882-76290901 MS.gene066701:CDS 40.0%
TCGTCTTACCCATAGATTGT+AGG + chr4.3:76291486-76291505 MS.gene066701:CDS 40.0%
TGAGATCCCATGGAGATAAA+TGG - chr4.3:76290695-76290714 None:intergenic 40.0%
TGGAAAGAAGTTAAGTGTGG+AGG - chr4.3:76290805-76290824 None:intergenic 40.0%
TGGTGGAAAGAAGTTAAGTG+TGG - chr4.3:76290808-76290827 None:intergenic 40.0%
TTACCGTAATAGTTGAACCG+TGG - chr4.3:76291761-76291780 None:intergenic 40.0%
!! AAGCGCTTTTAAGTCACCTT+TGG + chr4.3:76291414-76291433 MS.gene066701:CDS 40.0%
!! AATGGTAGTGTTTTCTGCTG+TGG - chr4.3:76290925-76290944 None:intergenic 40.0%
!! CATCACCTTGATTTGTTGCT+TGG - chr4.3:76290650-76290669 None:intergenic 40.0%
AAATTGGCACCAACCCAAAG+AGG + chr4.3:76290723-76290742 MS.gene066701:CDS 45.0%
ACGAATGCAGACCTTTCTTC+TGG - chr4.3:76291470-76291489 None:intergenic 45.0%
CTACCTGGCTCCGATATAAA+CGG - chr4.3:76291704-76291723 None:intergenic 45.0%
TACCTGGCTCCGATATAAAC+GGG - chr4.3:76291703-76291722 None:intergenic 45.0%
TAGTTCCTTCTTTCGAACGC+TGG + chr4.3:76291180-76291199 MS.gene066701:CDS 45.0%
TCTTGGGCTGAAATCTCACA+AGG + chr4.3:76291140-76291159 MS.gene066701:CDS 45.0%
TGAAAGGAAACCGTATGGGA+CGG - chr4.3:76291223-76291242 None:intergenic 45.0%
TGATAGCAAGCAGTTCACCA+CGG + chr4.3:76291741-76291760 MS.gene066701:CDS 45.0%
TGGTTGAAGTATGTCGGCAA+TGG - chr4.3:76290943-76290962 None:intergenic 45.0%
! GTGTAGACGGCAAGTCATTT+TGG + chr4.3:76291105-76291124 MS.gene066701:CDS 45.0%
!! ATGGAACATGAGGCGTTGTT+AGG - chr4.3:76290867-76290886 None:intergenic 45.0%
AACTTCTTTCCACCACTCGC+TGG + chr4.3:76290813-76290832 MS.gene066701:CDS 50.0%
ACCTGGCTCCGATATAAACG+GGG - chr4.3:76291702-76291721 None:intergenic 50.0%
ACTTGCCGTCTACACACACA+TGG - chr4.3:76291100-76291119 None:intergenic 50.0%
AGGAAACGACCCTATGTCCA+TGG + chr4.3:76291925-76291944 MS.gene066701:CDS 50.0%
ATTACAAGACGACCAGCGAG+TGG - chr4.3:76290828-76290847 None:intergenic 50.0%
GGAAACCAGCGTTCGAAAGA+AGG - chr4.3:76291188-76291207 None:intergenic 50.0%
! GTTATTGTGAATGGTGGGGC+AGG + chr4.3:76291848-76291867 MS.gene066701:CDS 50.0%
! TAGTGTTTTCTGCTGTGGCG+TGG - chr4.3:76290920-76290939 None:intergenic 50.0%
ACCCCGTTTATATCGGAGCC+AGG + chr4.3:76291698-76291717 MS.gene066701:CDS 55.0%
ACAAGACGACCAGCGAGTGG+TGG - chr4.3:76290825-76290844 None:intergenic 60.0%
GGGAGTGGAATCGGAGCAAC+TGG + chr4.3:76291545-76291564 MS.gene066701:CDS 60.0%
! GTGAATGGTGGGGCAGGAGA+AGG + chr4.3:76291854-76291873 MS.gene066701:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 76290612 76291988 76290612 ID=MS.gene066701
chr4.3 mRNA 76290612 76291988 76290612 ID=MS.gene066701.t1;Parent=MS.gene066701
chr4.3 exon 76290612 76291988 76290612 ID=MS.gene066701.t1.exon1;Parent=MS.gene066701.t1
chr4.3 CDS 76290612 76291988 76290612 ID=cds.MS.gene066701.t1;Parent=MS.gene066701.t1
Gene Sequence

>MS.gene066701

ATGACAGAGATTCTACTTATCTCAACTACTACAATCCAAGCAACAAATCAAGGTGATGACAAATCAACTCACAAAATTATCCATTTATCTCCATGGGATCTCAAATTTCTCAAATTGGCACCAACCCAAAGAGGTCTTCTTTTCCATAAACCCAAAACAAACCAAATAGACCATCTTAAACAAACACTTTCCTCCACACTTAACTTCTTTCCACCACTCGCTGGTCGTCTTGTAATAACACAACACGACGAACCTAACAACGCCTCATGTTCCATCATTTGTAACAACGCTGGTGCATTGTTTGTCCACGCCACAGCAGAAAACACTACCATTGCCGACATACTTCAACCAAAGTATGTTCCTCCCATTGTTTCTTCATTATTCTTACTCAATGATGTGAAAAGCTACGAAGGCACGTCACAACCACTACTTGCAGTACAAGTAACAGAGTTAATCGACGGTATCTTCATTGGTTTTGCAATTAACCATGTGTGTGTAGACGGCAAGTCATTTTGGCATTTCATCAACTCTTGGGCTGAAATCTCACAAGGTTCCAATAAGCCAACAATAGTTCCTTCTTTCGAACGCTGGTTTCCAAACAATGTTAACCGTCCCATACGGTTTCCTTTCACAAAGGATGTGCAAAATCAACAGTGTGAAGTTTCGTCTAATCGAATCTTTCACTTTACAAAGGAACAAATTCTGCAACTCAAATCAAAAGCTAATGCAGAAATTAGTAGTGGTAGTTGTAGTAGTAGTAGTAGTAATAGTAATGATAAAATAATAATGATATCTTCATTGCAAGCGCTTTTAAGTCACCTTTGGCGTTTAATTATAAGTAAACAAAATCTTAAACCAGAAGAAAGGTCTGCATTCGTCTTACCCATAGATTGTAGGACAAGAATGTGTCCAAAGTTAGAAGAGAATTATTTTGGGAGTGGAATCGGAGCAACTGGATATGTAATAATGCAAAAAGGGGAATTAATGGAAAGTGGGATTGGTAAAATTGCTATGGAAATGAACAAGGAGATTTCATCAAAATCTCATCATGAGAAGGTAATGAGTGATATTGAATCTTGGTTGAAAACCCCGTTTATATCGGAGCCAGGTAGATCATCAAGTAATCTTTTGATAGCAAGCAGTTCACCACGGTTCAACTATTACGGTAATGACTTTGGTTGGGGTAAGCCTATTGCAGTTAGAAATGGGGTTGGACTGCAAAGAAATATTGGTAGAGTTATTGTGAATGGTGGGGCAGGAGAAGGTAGTGTTGATATTGAAGTTTGTCTTCCTTATGATATTTTGGATGCTATAGGAAACGACCCTATGTCCATGGATTCTAGTTTCATCAATAATGTTATTGGAACACATGCTTGA
Protein sequence

>MS.gene066701.t1

MTEILLISTTTIQATNQGDDKSTHKIIHLSPWDLKFLKLAPTQRGLLFHKPKTNQIDHLKQTLSSTLNFFPPLAGRLVITQHDEPNNASCSIICNNAGALFVHATAENTTIADILQPKYVPPIVSSLFLLNDVKSYEGTSQPLLAVQVTELIDGIFIGFAINHVCVDGKSFWHFINSWAEISQGSNKPTIVPSFERWFPNNVNRPIRFPFTKDVQNQQCEVSSNRIFHFTKEQILQLKSKANAEISSGSCSSSSSNSNDKIIMISSLQALLSHLWRLIISKQNLKPEERSAFVLPIDCRTRMCPKLEENYFGSGIGATGYVIMQKGELMESGIGKIAMEMNKEISSKSHHEKVMSDIESWLKTPFISEPGRSSSNLLIASSSPRFNYYGNDFGWGKPIAVRNGVGLQRNIGRVIVNGGAGEGSVDIEVCLPYDILDAIGNDPMSMDSSFINNVIGTHA