AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene011796

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011796.t1 MTR_4g094842 91.241 411 33 2 1 410 39 447 0.0 764
MS.gene011796.t1 MTR_4g094835 60.732 410 143 7 3 407 46 442 2.20e-174 494
MS.gene011796.t1 MTR_8g041770 58.537 410 157 5 1 409 42 439 3.12e-165 471
MS.gene011796.t1 MTR_4g022825 57.831 415 155 7 2 409 37 438 1.67e-163 467
MS.gene011796.t1 MTR_4g094838 58.637 411 140 7 3 407 42 428 1.90e-161 461
MS.gene011796.t1 MTR_0190s0020 55.257 409 174 4 1 409 41 440 4.01e-160 458
MS.gene011796.t1 MTR_3g088420 58.049 410 153 8 3 409 38 431 1.33e-159 457
MS.gene011796.t1 MTR_4g094832 63.380 355 118 5 58 407 79 426 6.03e-159 455
MS.gene011796.t1 MTR_4g022720 58.495 412 152 8 2 409 37 433 6.84e-158 452
MS.gene011796.t1 MTR_4g022800 56.938 418 156 8 2 409 37 440 7.95e-157 450
MS.gene011796.t1 MTR_8g079710 57.421 411 147 9 3 402 43 436 8.13e-157 450
MS.gene011796.t1 MTR_4g022670 56.115 417 160 8 2 409 37 439 1.05e-154 444
MS.gene011796.t1 MTR_3g026310 55.582 421 159 8 3 408 38 445 1.35e-154 444
MS.gene011796.t1 MTR_7g026860 56.490 416 157 8 2 409 37 436 7.62e-154 442
MS.gene011796.t1 MTR_4g094828 53.883 412 167 8 3 403 43 442 1.67e-152 439
MS.gene011796.t1 MTR_4g022290 53.865 414 160 9 3 404 44 438 4.10e-148 428
MS.gene011796.t1 MTR_4g022760 50.962 416 139 7 2 409 37 395 4.41e-131 383
MS.gene011796.t1 MTR_3g101900 41.706 422 218 13 3 412 44 449 2.33e-96 296
MS.gene011796.t1 MTR_6g016815 44.359 390 140 8 19 407 64 377 4.82e-95 290
MS.gene011796.t1 MTR_3g101930 41.905 420 221 11 3 409 44 453 6.41e-95 292
MS.gene011796.t1 MTR_3g101960 40.476 420 228 10 3 412 43 450 3.13e-87 273
MS.gene011796.t1 MTR_3g101970 39.100 422 221 10 2 412 42 438 2.51e-86 270
MS.gene011796.t1 MTR_3g102020 39.379 419 226 10 3 407 43 447 3.98e-84 265
MS.gene011796.t1 MTR_3g102010 38.353 425 235 10 3 414 44 454 1.29e-81 258
MS.gene011796.t1 MTR_3g070170 35.440 443 225 9 3 407 47 466 2.96e-81 258
MS.gene011796.t1 MTR_8g040780 53.488 215 97 2 195 406 13 227 5.97e-76 236
MS.gene011796.t1 MTR_5g081960 33.190 464 229 10 3 407 41 482 6.75e-72 234
MS.gene011796.t1 MTR_4g015180 32.762 467 230 10 3 408 41 484 1.84e-68 225
MS.gene011796.t1 MTR_6g045553 32.805 442 243 12 2 407 48 471 3.60e-59 200
MS.gene011796.t1 MTR_7g026850 61.688 154 47 3 2 154 20 162 5.81e-59 190
MS.gene011796.t1 MTR_8g040760 56.831 183 70 5 2 183 40 214 7.49e-57 186
MS.gene011796.t1 MTR_4g094825 54.913 173 71 3 3 175 106 271 2.85e-55 187
MS.gene011796.t1 MTR_4g094825 56.291 151 54 2 258 407 248 387 9.10e-49 171
MS.gene011796.t1 MTR_7g026770 50.515 194 70 3 116 307 132 301 6.62e-50 171
MS.gene011796.t1 MTR_3g102030 33.038 339 184 10 35 365 17 320 2.48e-46 162
MS.gene011796.t1 MTR_6g034495 63.025 119 41 1 35 153 45 160 1.37e-45 155
MS.gene011796.t1 MTR_6g034485 55.469 128 53 2 161 288 1 124 4.90e-43 147
MS.gene011796.t1 MTR_4g007540 28.029 421 243 16 8 398 34 424 1.16e-38 144
MS.gene011796.t1 MTR_2g105330 27.845 413 253 17 8 398 34 423 2.93e-35 135
MS.gene011796.t1 MTR_2g064495 27.603 413 254 17 8 398 34 423 4.60e-35 134
MS.gene011796.t1 MTR_7g026830 62.222 90 34 0 51 140 8 97 3.93e-33 120
MS.gene011796.t1 MTR_8g075610 26.000 400 240 14 24 400 47 413 5.79e-32 125
MS.gene011796.t1 MTR_8g096530 30.199 351 208 13 22 360 57 382 6.55e-32 126
MS.gene011796.t1 MTR_3g098980 28.382 377 238 12 24 392 66 418 1.63e-29 119
MS.gene011796.t1 MTR_3g026950 27.294 425 240 15 12 396 42 437 7.56e-28 114
MS.gene011796.t1 MTR_1g081660 24.672 381 243 14 8 374 57 407 2.27e-23 102
MS.gene011796.t1 MTR_4g013050 26.699 412 227 13 15 402 44 404 4.44e-23 100
MS.gene011796.t1 MTR_4g127650 25.057 435 254 17 6 400 46 448 5.84e-23 100
MS.gene011796.t1 MTR_4g097090 25.476 420 253 14 7 392 47 440 1.03e-22 100
MS.gene011796.t1 MTR_7g027175 26.263 396 255 13 15 398 59 429 1.03e-22 99.8
MS.gene011796.t1 MTR_1g040195 27.066 351 224 13 64 398 71 405 1.26e-22 99.8
MS.gene011796.t1 MTR_3g049150 26.456 412 237 17 21 396 52 433 2.53e-21 95.9
MS.gene011796.t1 MTR_1g040215 25.341 367 228 15 24 375 64 399 7.28e-21 94.7
MS.gene011796.t1 MTR_7g084940 27.424 361 221 14 15 360 58 392 8.13e-21 94.4
MS.gene011796.t1 MTR_3g071320 27.362 307 188 11 6 295 42 330 1.51e-20 93.6
MS.gene011796.t1 MTR_1g040175 25.205 365 241 13 19 375 59 399 1.99e-20 93.2
MS.gene011796.t1 MTR_7g014250 28.010 407 224 18 7 374 53 429 2.19e-20 93.2
MS.gene011796.t1 MTR_7g014230 28.238 386 220 16 24 372 64 429 5.17e-20 92.0
MS.gene011796.t1 MTR_7g026780 44.037 109 36 3 301 409 26 109 9.06e-20 84.7
MS.gene011796.t1 MTR_6g015315 27.653 311 198 11 113 401 140 445 1.03e-19 91.3
MS.gene011796.t1 MTR_6g015815 24.691 405 261 13 21 400 67 452 1.51e-19 90.9
MS.gene011796.t1 MTR_2g089765 27.044 318 185 13 112 393 159 465 1.78e-19 90.5
MS.gene011796.t1 MTR_7g085930 23.799 437 263 12 6 398 41 451 1.97e-19 90.5
MS.gene011796.t1 MTR_3g086020 24.190 401 243 14 6 392 43 396 2.64e-19 89.7
MS.gene011796.t1 MTR_6g015320 25.733 307 202 9 113 398 143 444 4.29e-19 89.4
MS.gene011796.t1 MTR_5g076700 23.058 412 275 16 2 392 36 426 1.97e-18 87.4
MS.gene011796.t1 MTR_6g015850 25.407 307 204 7 113 399 147 448 2.39e-18 87.0
MS.gene011796.t1 MTR_7g015860 24.528 424 251 15 24 403 64 462 2.49e-18 87.0
MS.gene011796.t1 MTR_4g055170 28.013 307 187 10 14 308 59 343 3.36e-18 86.7
MS.gene011796.t1 MTR_7g114550 23.474 426 277 18 2 403 36 436 9.67e-18 85.1
MS.gene011796.t1 MTR_3g028210 24.324 407 259 14 17 392 60 448 1.16e-17 85.1
MS.gene011796.t1 MTR_6g015830 23.529 408 262 11 21 400 67 452 1.21e-17 85.1
MS.gene011796.t1 MTR_7g026800 37.857 140 57 2 189 327 27 137 1.67e-17 80.5
MS.gene011796.t1 MTR_3g465980 26.263 297 203 10 1 291 43 329 2.50e-17 84.0
MS.gene011796.t1 MTR_5g084850 24.165 389 240 16 6 377 41 391 3.73e-17 83.6
MS.gene011796.t1 MTR_5g090830 26.330 376 210 14 24 361 66 412 5.00e-17 83.2
MS.gene011796.t1 MTR_7g114470 23.628 419 265 17 2 392 37 428 5.30e-17 83.2
MS.gene011796.t1 MTR_5g090810 25.532 376 213 14 24 361 66 412 7.00e-17 82.8
MS.gene011796.t1 MTR_8g079970 22.475 396 245 13 6 377 41 398 8.19e-17 82.4
MS.gene011796.t1 MTR_8g012750 25.668 374 228 12 24 360 62 422 8.64e-17 82.4
MS.gene011796.t1 MTR_7g114490 23.841 453 274 20 2 412 36 459 1.18e-16 82.0
MS.gene011796.t1 MTR_8g079980 22.251 391 251 12 6 377 41 397 1.48e-16 81.6
MS.gene011796.t1 MTR_7g086000 26.355 406 254 17 24 400 60 449 1.93e-16 81.3
MS.gene011796.t1 MTR_7g114520 24.074 378 252 15 9 374 53 407 2.15e-16 81.3
MS.gene011796.t1 MTR_6g053810 25.532 282 192 8 18 292 65 335 2.17e-16 81.3
MS.gene011796.t1 MTR_7g085990 27.365 296 175 11 6 278 37 315 2.18e-16 81.3
MS.gene011796.t1 MTR_5g084870 22.137 393 244 13 6 377 41 392 2.25e-16 80.9
MS.gene011796.t1 MTR_7g014160 26.442 416 242 18 24 397 69 462 3.65e-16 80.5
MS.gene011796.t1 MTR_3g087640 26.392 413 249 16 24 398 67 462 4.42e-16 80.5
MS.gene011796.t1 MTR_7g013850 23.350 394 232 13 19 363 63 435 5.85e-16 80.1
MS.gene011796.t1 MTR_7g014360 27.737 274 154 11 113 363 90 342 7.57e-16 79.0
MS.gene011796.t1 MTR_7g114560 23.059 425 280 18 2 403 36 436 8.54e-16 79.3
MS.gene011796.t1 MTR_5g026320 23.487 413 254 14 21 406 71 448 2.46e-15 77.8
MS.gene011796.t1 MTR_7g084910 25.871 402 229 17 15 393 59 414 2.50e-15 77.8
MS.gene011796.t1 MTR_3g450710 24.235 392 234 16 7 361 46 411 3.17e-15 77.8
MS.gene011796.t1 MTR_7g014610 24.515 412 246 18 24 392 68 457 5.51e-15 77.0
MS.gene011796.t1 MTR_1g053315 23.333 360 227 16 24 361 78 410 5.71e-15 77.0
MS.gene011796.t1 MTR_8g079950 23.469 392 260 12 6 384 41 405 6.02e-15 76.6
MS.gene011796.t1 MTR_3g465990 25.965 285 194 9 13 291 56 329 6.46e-15 76.6
MS.gene011796.t1 MTR_2g089670 26.757 370 216 16 24 360 82 429 9.42e-15 76.3
MS.gene011796.t1 MTR_3g049140 26.033 242 143 8 185 408 18 241 9.99e-15 73.9
MS.gene011796.t1 MTR_7g056087 24.752 303 197 8 7 292 41 329 1.53e-14 75.5
MS.gene011796.t1 MTR_2g089735 25.157 318 201 13 112 402 148 455 4.31e-14 74.3
MS.gene011796.t1 MTR_5g090880 23.907 389 238 15 8 361 49 414 1.30e-13 72.8
MS.gene011796.t1 MTR_3g465930 24.911 281 191 6 19 291 64 332 1.82e-13 72.4
MS.gene011796.t1 MTR_1g040315 25.253 297 177 12 2 279 35 305 3.17e-13 71.2
MS.gene011796.t1 MTR_2g007880 24.578 415 253 17 24 414 74 452 1.66e-12 69.3
MS.gene011796.t1 MTR_7g014620 24.105 419 224 15 24 398 54 422 2.75e-12 68.6
MS.gene011796.t1 MTR_1g094790 24.700 417 258 16 16 407 52 437 3.71e-12 68.2
MS.gene011796.t1 MTR_2g015430 22.794 272 197 6 104 369 128 392 4.48e-12 67.8
MS.gene011796.t1 MTR_3g465910 25.623 281 187 9 19 291 63 329 3.55e-11 65.5
MS.gene011796.t1 MTR_7g026880 50.000 52 24 1 358 409 2 51 3.87e-11 58.5
MS.gene011796.t1 MTR_7g026870 53.704 54 25 0 267 320 98 151 8.51e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011796.t1 AT5G67150 42.482 419 218 13 3 412 43 447 6.93e-102 310
MS.gene011796.t1 AT3G50300 42.105 399 209 11 3 393 41 425 4.88e-91 282
MS.gene011796.t1 AT3G50270 40.049 412 230 10 3 407 43 444 8.12e-91 281
MS.gene011796.t1 AT3G50280 39.078 412 231 9 3 407 41 439 3.64e-90 280
MS.gene011796.t1 AT5G67160 39.853 409 217 11 3 406 43 427 4.27e-85 266
MS.gene011796.t1 AT5G38130 39.702 403 217 12 3 396 61 446 4.87e-85 267
MS.gene011796.t1 AT5G42830 38.095 420 237 10 3 412 44 450 4.14e-83 262
MS.gene011796.t1 AT5G07860 38.406 414 237 8 3 409 45 447 7.18e-83 261
MS.gene011796.t1 AT5G07850 38.756 418 234 9 3 409 45 451 4.27e-82 259
MS.gene011796.t1 AT5G07870 37.644 433 226 11 3 409 45 459 2.65e-80 255
MS.gene011796.t1 AT5G01210 35.360 444 231 7 3 408 41 466 3.24e-78 250
MS.gene011796.t1 AT2G39980 33.846 455 232 8 3 408 43 477 8.04e-78 249
MS.gene011796.t1 AT3G50280 38.019 313 185 4 100 407 1 309 3.91e-68 219
MS.gene011796.t1 AT5G23940 33.021 427 249 10 2 407 38 448 4.19e-67 221
MS.gene011796.t1 AT3G50290 42.963 270 142 7 97 358 4 269 5.73e-58 191
MS.gene011796.t1 AT5G48930 27.861 402 256 14 8 392 34 418 1.16e-35 136
MS.gene011796.t1 AT3G48720 25.250 400 272 9 7 399 44 423 5.07e-27 112
MS.gene011796.t1 AT2G19070 25.653 421 270 13 6 400 40 443 1.31e-26 111
MS.gene011796.t1 AT5G41040 27.273 407 247 16 21 409 66 441 1.45e-26 111
MS.gene011796.t1 AT5G41040 27.273 407 247 16 21 409 82 457 1.79e-26 110
MS.gene011796.t1 AT2G40230 27.221 349 233 8 23 365 58 391 5.58e-24 103
MS.gene011796.t1 AT5G57840 26.950 423 244 17 1 392 34 422 1.53e-23 102
MS.gene011796.t1 AT5G63560 25.432 405 247 12 7 392 44 412 1.94e-23 102
MS.gene011796.t1 AT3G29590 24.750 400 250 15 30 402 67 442 2.42e-23 102
MS.gene011796.t1 AT5G17540 27.123 424 254 19 2 398 35 430 8.11e-23 100
MS.gene011796.t1 AT3G29635 25.765 392 248 14 24 392 67 438 2.08e-22 99.4
MS.gene011796.t1 AT1G03940 25.980 408 244 16 21 393 63 447 6.19e-22 97.8
MS.gene011796.t1 AT3G03480 26.368 402 238 16 2 380 45 411 2.85e-21 95.9
MS.gene011796.t1 AT5G61160 25.862 290 174 10 110 372 139 414 7.77e-20 91.7
MS.gene011796.t1 AT1G24430 24.745 392 243 16 6 384 45 397 2.29e-19 90.1
MS.gene011796.t1 AT1G24420 25.195 385 245 16 34 408 75 426 4.80e-19 89.0
MS.gene011796.t1 AT1G24420 25.195 385 245 16 34 408 75 426 4.80e-19 89.0
MS.gene011796.t1 AT5G07080 27.063 303 186 12 4 291 49 331 6.11e-19 89.0
MS.gene011796.t1 AT1G65450 27.010 311 200 13 64 360 103 400 1.01e-18 88.2
MS.gene011796.t1 AT3G29670 22.249 409 261 13 8 392 58 433 1.69e-18 87.4
MS.gene011796.t1 AT3G47170 27.402 281 177 10 28 291 74 344 2.26e-18 87.4
MS.gene011796.t1 AT5G39050 24.834 302 184 12 124 393 160 450 8.94e-18 85.5
MS.gene011796.t1 AT5G16410 26.050 357 217 10 24 361 91 419 2.79e-17 84.0
MS.gene011796.t1 AT5G23970 25.060 419 242 18 16 407 44 417 3.60e-17 83.6
MS.gene011796.t1 AT3G30280 23.430 414 235 16 5 384 43 408 1.20e-16 82.0
MS.gene011796.t1 AT5G39080 23.961 409 249 15 21 392 62 445 1.30e-16 82.0
MS.gene011796.t1 AT3G26040 23.934 422 274 15 6 412 41 430 1.80e-16 81.3
MS.gene011796.t1 AT1G32910 25.668 374 227 16 12 361 53 399 4.78e-16 80.1
MS.gene011796.t1 AT4G15390 22.196 428 271 15 5 407 45 435 7.58e-16 79.3
MS.gene011796.t1 AT4G15390 22.196 428 271 15 5 407 45 435 7.58e-16 79.3
MS.gene011796.t1 AT3G29680 24.809 262 160 9 124 361 152 400 4.40e-15 77.0
MS.gene011796.t1 AT1G28680 25.575 348 225 15 27 363 65 389 2.08e-14 75.1
MS.gene011796.t1 AT1G03390 24.119 369 246 15 7 361 59 407 2.66e-14 74.7
MS.gene011796.t1 AT5G39090 27.612 268 169 13 110 360 140 399 3.15e-14 74.7
MS.gene011796.t1 AT1G65450 27.347 245 158 9 127 360 1 236 8.29e-14 72.0
MS.gene011796.t1 AT1G28680 26.316 304 193 13 27 320 65 347 2.03e-13 71.6
MS.gene011796.t1 AT5G47980 24.590 427 262 20 6 407 41 432 3.52e-13 71.2
MS.gene011796.t1 AT2G23510 23.410 393 258 16 4 378 48 415 1.28e-12 69.7

Find 73 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCAAGGTTTGATGTTTA+TGG 0.150931 8.1:+26699342 MS.gene011796:CDS
TTGTCTTTCTCATGATATTT+TGG 0.231632 8.1:+26699479 MS.gene011796:CDS
CAATACATGATTTCTTCTTC+TGG 0.269181 8.1:-26699057 None:intergenic
CTCAATTTATACATATCTTT+TGG 0.280596 8.1:+26699520 MS.gene011796:CDS
ATTTGGTTTGAGGTGTCTTT+TGG 0.287416 8.1:-26698349 None:intergenic
GGAACGATAACTATGTTTAA+TGG 0.296463 8.1:+26699426 MS.gene011796:CDS
GAACGATAACTATGTTTAAT+GGG 0.345252 8.1:+26699427 MS.gene011796:CDS
CAAGTTACTGAGCTTGTTGA+TGG 0.358436 8.1:+26698658 MS.gene011796:CDS
TTGTCGATGGTAAGTCGTTT+TGG 0.363142 8.1:+26698713 MS.gene011796:CDS
TTACTTGCTGAAGGAGCGTT+TGG 0.366525 8.1:+26699180 MS.gene011796:CDS
ACAATGGAAGGAACATAGTT+TGG 0.370467 8.1:-26698568 None:intergenic
GCTTAGTAGTGTTAAGGCTT+TGG 0.398296 8.1:+26699308 MS.gene011796:CDS
CAAGTTGAAATGAACCAAAA+AGG 0.407254 8.1:+26698301 None:intergenic
TGCATCCTTTGTAACAATCT+AGG 0.410406 8.1:+26698490 MS.gene011796:CDS
TTATGGTAACGACTTTGGTT+GGG 0.416720 8.1:+26699359 MS.gene011796:CDS
TGTTTAGTCATTGATGTTAG+AGG 0.419291 8.1:+26699078 MS.gene011796:CDS
TTACTTCGGAAATGCATTGC+AGG 0.421621 8.1:+26699128 MS.gene011796:CDS
AGGTAGGCTTAGTAGTGTTA+AGG 0.422729 8.1:+26699302 MS.gene011796:CDS
TTTGGCATTTCATCAATTCA+TGG 0.423295 8.1:+26698731 MS.gene011796:CDS
CAAAAGATATGTATAAATTG+AGG 0.446238 8.1:-26699519 None:intergenic
TGCATTTCCGAAGTAATTCT+CGG 0.452398 8.1:-26699121 None:intergenic
TTTATGGTAACGACTTTGGT+TGG 0.458812 8.1:+26699358 MS.gene011796:CDS
GAGGTGTTGGATTTGGTTTG+AGG 0.469311 8.1:-26698359 None:intergenic
AGCCTGTTGCTGTTAGAAGT+GGG 0.474750 8.1:+26699385 MS.gene011796:CDS
AAAAGATATGTATAAATTGA+GGG 0.486716 8.1:-26699518 None:intergenic
ATCAATCATGTTGTTGTCGA+TGG 0.491424 8.1:+26698700 MS.gene011796:CDS
CTTTGGCAAGCTGTGATTCG+TGG 0.491749 8.1:+26699021 MS.gene011796:CDS
TGAAGAGAAACTATGAAAGT+TGG 0.492121 8.1:+26699256 MS.gene011796:CDS
GTTTAGTCATTGATGTTAGA+GGG 0.492217 8.1:+26699079 MS.gene011796:CDS
TATGGTAACGACTTTGGTTG+GGG 0.494123 8.1:+26699360 MS.gene011796:CDS
TTAAGTGTTGGAAGCTTCGA+TGG 0.495846 8.1:-26698781 None:intergenic
AGCTTCCAACACTTAATCGT+TGG 0.496175 8.1:+26698788 MS.gene011796:CDS
GTGAAATGGAAAATCCTCTC+AGG 0.496490 8.1:-26698910 None:intergenic
AAGCCTGTTGCTGTTAGAAG+TGG 0.503104 8.1:+26699384 MS.gene011796:CDS
AGCCTACCTTGAAATAACCT+TGG 0.508363 8.1:-26699288 None:intergenic
CCGAAGTAATTCTCGGGCAA+AGG 0.515823 8.1:-26699114 None:intergenic
GAAGAGAAACTATGAAAGTT+GGG 0.516810 8.1:+26699257 MS.gene011796:CDS
ATGTTTAATGGGGCAGAAGA+AGG 0.517612 8.1:+26699438 MS.gene011796:CDS
AGAAAACAAGAGTTGATGAA+AGG 0.528232 8.1:-26698390 None:intergenic
GAAAACAAGAGTTGATGAAA+GGG 0.528741 8.1:-26698389 None:intergenic
ATGAGACGACCGGTGAGGGG+TGG 0.536608 8.1:-26698415 None:intergenic
GCATTTCCGAAGTAATTCTC+GGG 0.538714 8.1:-26699120 None:intergenic
AAGTAATTCTCGGGCAAAGG+TGG 0.543022 8.1:-26699111 None:intergenic
GATGTTTATGGTAACGACTT+TGG 0.543853 8.1:+26699354 MS.gene011796:CDS
AATGATGAGACGACCGGTGA+GGG 0.544298 8.1:-26698419 None:intergenic
CCTTTGCCCGAGAATTACTT+CGG 0.547729 8.1:+26699114 MS.gene011796:CDS
ATCCAAGGTTATTTCAAGGT+AGG 0.548103 8.1:+26699286 MS.gene011796:CDS
GGAAATGCATTGCAGGTTGG+TGG 0.549600 8.1:+26699135 MS.gene011796:CDS
TGAGACGACCGGTGAGGGGT+GGG 0.555527 8.1:-26698414 None:intergenic
TTCGGAAATGCATTGCAGGT+TGG 0.562745 8.1:+26699132 MS.gene011796:CDS
GTAACTTGAACTGCAAGCAA+TGG 0.569233 8.1:-26698643 None:intergenic
GCCTGTTGCTGTTAGAAGTG+GGG 0.572234 8.1:+26699386 MS.gene011796:CDS
AGAAACCAACGATTAAGTGT+TGG 0.580659 8.1:-26698793 None:intergenic
GCAAAGGAATTACTTGCTGA+AGG 0.591175 8.1:+26699171 MS.gene011796:CDS
TTACCATAAACATCAAACCT+TGG 0.591743 8.1:-26699345 None:intergenic
AAATGGAAAATCCTCTCAGG+TGG 0.591822 8.1:-26698907 None:intergenic
CCTGTTGCTGTTAGAAGTGG+GGG 0.592205 8.1:+26699387 MS.gene011796:CDS
TATAAAATTTCCATCCACAA+AGG 0.594467 8.1:+26698837 MS.gene011796:CDS
TGGCAACTAGTAGTTCTCCA+AGG 0.596495 8.1:+26699328 MS.gene011796:CDS
CACGAATCACAGCTTGCCAA+AGG 0.614383 8.1:-26699020 None:intergenic
AAAATTTCCATCCACAAAGG+AGG 0.623863 8.1:+26698840 MS.gene011796:CDS
AAGATGATAGCTTCACATTG+TGG 0.626657 8.1:+26699225 MS.gene011796:CDS
ACGCACCTAGATTGTTACAA+AGG 0.626814 8.1:-26698495 None:intergenic
ATGTTCAATGATGAGACGAC+CGG 0.630993 8.1:-26698425 None:intergenic
GTTACAAAGGATGCAACATG+AGG 0.639280 8.1:-26698482 None:intergenic
GAAATCAAAAGCTAACGAAG+AGG 0.643741 8.1:+26698954 MS.gene011796:CDS
AACGATAACTATGTTTAATG+GGG 0.647537 8.1:+26699428 MS.gene011796:CDS
GAACATCAAGAAGAAGAAGA+CGG 0.649390 8.1:+26698442 MS.gene011796:CDS
ATGATGAGACGACCGGTGAG+GGG 0.660103 8.1:-26698418 None:intergenic
CAATGATGAGACGACCGGTG+AGG 0.661803 8.1:-26698420 None:intergenic
TCATGGTCTGAAATCTCACG+TGG 0.682326 8.1:+26698748 MS.gene011796:CDS
AAAACTCTATTCCACCTGAG+AGG 0.686471 8.1:+26698896 MS.gene011796:CDS
TGGTGTGAAAGTGAAAGCAA+AGG 0.742441 8.1:+26699155 MS.gene011796:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTTTTTTACTTTATTAAA+CGG - chr8.1:26698607-26698626 None:intergenic 10.0%
!! AAAAGATATGTATAAATTGA+GGG - chr8.1:26699521-26699540 None:intergenic 15.0%
!! AATAAAGTAAAAAACTACGA+AGG + chr8.1:26698610-26698629 MS.gene011796:CDS 20.0%
!! CAAAAGATATGTATAAATTG+AGG - chr8.1:26699522-26699541 None:intergenic 20.0%
!!! AGAAGATATTTTGTTTGTAT+TGG - chr8.1:26698980-26698999 None:intergenic 20.0%
!!! CTCAATTTATACATATCTTT+TGG + chr8.1:26699520-26699539 MS.gene011796:CDS 20.0%
! AACGATAACTATGTTTAATG+GGG + chr8.1:26699428-26699447 MS.gene011796:CDS 25.0%
! GAACGATAACTATGTTTAAT+GGG + chr8.1:26699427-26699446 MS.gene011796:CDS 25.0%
! TATAAAATTTCCATCCACAA+AGG + chr8.1:26698837-26698856 MS.gene011796:CDS 25.0%
!! AAAAATCCAAGGTTATTTCA+AGG + chr8.1:26699282-26699301 MS.gene011796:CDS 25.0%
!! TAATTTTCTCCTTTGTGAAA+TGG - chr8.1:26698927-26698946 None:intergenic 25.0%
!! TTGTCTTTCTCATGATATTT+TGG + chr8.1:26699479-26699498 MS.gene011796:CDS 25.0%
!! TTTGTGGATGGAAATTTTAT+AGG - chr8.1:26698838-26698857 None:intergenic 25.0%
CAATACATGATTTCTTCTTC+TGG - chr8.1:26699060-26699079 None:intergenic 30.0%
GAAGAGAAACTATGAAAGTT+GGG + chr8.1:26699257-26699276 MS.gene011796:CDS 30.0%
GGAACGATAACTATGTTTAA+TGG + chr8.1:26699426-26699445 MS.gene011796:CDS 30.0%
GTTTAGTCATTGATGTTAGA+GGG + chr8.1:26699079-26699098 MS.gene011796:CDS 30.0%
TAGTTTATGTTACTGTGCAT+GGG + chr8.1:26699544-26699563 MS.gene011796:intron 30.0%
TGAAGAGAAACTATGAAAGT+TGG + chr8.1:26699256-26699275 MS.gene011796:CDS 30.0%
TGTTTAGTCATTGATGTTAG+AGG + chr8.1:26699078-26699097 MS.gene011796:CDS 30.0%
TTACCATAAACATCAAACCT+TGG - chr8.1:26699348-26699367 None:intergenic 30.0%
TTAGTTTATGTTACTGTGCA+TGG + chr8.1:26699543-26699562 MS.gene011796:intron 30.0%
TTTGGCATTTCATCAATTCA+TGG + chr8.1:26698731-26698750 MS.gene011796:CDS 30.0%
! TATGAAAGAGAAGTCCTTTT+TGG - chr8.1:26698318-26698337 None:intergenic 30.0%
!! AGAAAACAAGAGTTGATGAA+AGG - chr8.1:26698393-26698412 None:intergenic 30.0%
!! GAAAACAAGAGTTGATGAAA+GGG - chr8.1:26698392-26698411 None:intergenic 30.0%
!! TCTTTCTCATGATATTTTGG+AGG + chr8.1:26699482-26699501 MS.gene011796:CDS 30.0%
!!! TTCTTTATTTTCCTCCTTTG+TGG - chr8.1:26698854-26698873 None:intergenic 30.0%
AAAAAAGAGCGAACAATGGA+AGG - chr8.1:26698583-26698602 None:intergenic 35.0%
AAAATTTCCATCCACAAAGG+AGG + chr8.1:26698840-26698859 MS.gene011796:CDS 35.0%
AAAGTTGGGTGAAAAATCCA+AGG + chr8.1:26699271-26699290 MS.gene011796:CDS 35.0%
AAGATGATAGCTTCACATTG+TGG + chr8.1:26699225-26699244 MS.gene011796:CDS 35.0%
ACAATGGAAGGAACATAGTT+TGG - chr8.1:26698571-26698590 None:intergenic 35.0%
AGAAACCAACGATTAAGTGT+TGG - chr8.1:26698796-26698815 None:intergenic 35.0%
ATAAGGGTAGTTGTGAGAAT+TGG - chr8.1:26699617-26699636 None:intergenic 35.0%
ATCAATCATGTTGTTGTCGA+TGG + chr8.1:26698700-26698719 MS.gene011796:CDS 35.0%
GAAATCAAAAGCTAACGAAG+AGG + chr8.1:26698954-26698973 MS.gene011796:CDS 35.0%
GAACATCAAGAAGAAGAAGA+CGG + chr8.1:26698442-26698461 MS.gene011796:CDS 35.0%
TAAGGGTAGTTGTGAGAATT+GGG - chr8.1:26699616-26699635 None:intergenic 35.0%
TCTCCAAGGTTTGATGTTTA+TGG + chr8.1:26699342-26699361 MS.gene011796:CDS 35.0%
TGCATCCTTTGTAACAATCT+AGG + chr8.1:26698490-26698509 MS.gene011796:CDS 35.0%
TGCATTTCCGAAGTAATTCT+CGG - chr8.1:26699124-26699143 None:intergenic 35.0%
! ATCCAAGGTTATTTCAAGGT+AGG + chr8.1:26699286-26699305 MS.gene011796:CDS 35.0%
! GAGGATTTTCCATTTCACAA+AGG + chr8.1:26698915-26698934 MS.gene011796:CDS 35.0%
! GATGTTTATGGTAACGACTT+TGG + chr8.1:26699354-26699373 MS.gene011796:CDS 35.0%
! TTATGGTAACGACTTTGGTT+GGG + chr8.1:26699359-26699378 MS.gene011796:CDS 35.0%
! TTATTTTCCTCCTTTGTGGA+TGG - chr8.1:26698850-26698869 None:intergenic 35.0%
! TTGTGTTTTCAGCTATTGCA+TGG - chr8.1:26698528-26698547 None:intergenic 35.0%
! TTTATGGTAACGACTTTGGT+TGG + chr8.1:26699358-26699377 MS.gene011796:CDS 35.0%
!! AAGCACTTTTAACTCACCTT+TGG + chr8.1:26699004-26699023 MS.gene011796:CDS 35.0%
!! CATGATATTTTGGAGGCTAT+GGG + chr8.1:26699489-26699508 MS.gene011796:CDS 35.0%
!! TCATGATATTTTGGAGGCTA+TGG + chr8.1:26699488-26699507 MS.gene011796:CDS 35.0%
!!! ATTTGGTTTGAGGTGTCTTT+TGG - chr8.1:26698352-26698371 None:intergenic 35.0%
!!! CTTGTTGATGGCATTTTCAT+TGG + chr8.1:26698670-26698689 MS.gene011796:CDS 35.0%
!!! TGTTTTTGAGGTGTTGGATT+TGG - chr8.1:26698369-26698388 None:intergenic 35.0%
!!! TTGATGAAAGGGTGTTTTTG+AGG - chr8.1:26698381-26698400 None:intergenic 35.0%
!!! TTTGGTTTGAGGTGTCTTTT+GGG - chr8.1:26698351-26698370 None:intergenic 35.0%
AAAACTCTATTCCACCTGAG+AGG + chr8.1:26698896-26698915 MS.gene011796:CDS 40.0%
AAATGGAAAATCCTCTCAGG+TGG - chr8.1:26698910-26698929 None:intergenic 40.0%
ACGAGGTGGCAATAGAATAA+GGG - chr8.1:26699633-26699652 None:intergenic 40.0%
ACGCACCTAGATTGTTACAA+AGG - chr8.1:26698498-26698517 None:intergenic 40.0%
AGAAAGCAGCAAGAGAAGAA+AGG - chr8.1:26699656-26699675 None:intergenic 40.0%
AGCCTACCTTGAAATAACCT+TGG - chr8.1:26699291-26699310 None:intergenic 40.0%
AGCTTCCAACACTTAATCGT+TGG + chr8.1:26698788-26698807 MS.gene011796:CDS 40.0%
AGGTAGGCTTAGTAGTGTTA+AGG + chr8.1:26699302-26699321 MS.gene011796:CDS 40.0%
ATGTTCAATGATGAGACGAC+CGG - chr8.1:26698428-26698447 None:intergenic 40.0%
ATGTTTAATGGGGCAGAAGA+AGG + chr8.1:26699438-26699457 MS.gene011796:CDS 40.0%
CGGAAAAAAAGAGCGAACAA+TGG - chr8.1:26698587-26698606 None:intergenic 40.0%
GCATTTCCGAAGTAATTCTC+GGG - chr8.1:26699123-26699142 None:intergenic 40.0%
GCTTAGTAGTGTTAAGGCTT+TGG + chr8.1:26699308-26699327 MS.gene011796:CDS 40.0%
GTAACTTGAACTGCAAGCAA+TGG - chr8.1:26698646-26698665 None:intergenic 40.0%
GTGAAATGGAAAATCCTCTC+AGG - chr8.1:26698913-26698932 None:intergenic 40.0%
GTTACAAAGGATGCAACATG+AGG - chr8.1:26698485-26698504 None:intergenic 40.0%
TGGTGTGAAAGTGAAAGCAA+AGG + chr8.1:26699155-26699174 MS.gene011796:CDS 40.0%
TTACTTCGGAAATGCATTGC+AGG + chr8.1:26699128-26699147 MS.gene011796:CDS 40.0%
TTTATGTTACTGTGCATGGG+CGG + chr8.1:26699547-26699566 MS.gene011796:intron 40.0%
! GCAAAGGAATTACTTGCTGA+AGG + chr8.1:26699171-26699190 MS.gene011796:CDS 40.0%
! TATGGTAACGACTTTGGTTG+GGG + chr8.1:26699360-26699379 MS.gene011796:CDS 40.0%
! TTGTCGATGGTAAGTCGTTT+TGG + chr8.1:26698713-26698732 MS.gene011796:CDS 40.0%
!! CAAGTTACTGAGCTTGTTGA+TGG + chr8.1:26698658-26698677 MS.gene011796:CDS 40.0%
!! TTAAGTGTTGGAAGCTTCGA+TGG - chr8.1:26698784-26698803 None:intergenic 40.0%
!!! AAAGGGTGTTTTTGAGGTGT+TGG - chr8.1:26698375-26698394 None:intergenic 40.0%
!!! GAGGTGTCTTTTGGGTTTTT+GGG - chr8.1:26698343-26698362 None:intergenic 40.0%
!!! TGAGGTGTCTTTTGGGTTTT+TGG - chr8.1:26698344-26698363 None:intergenic 40.0%
AAGTAATTCTCGGGCAAAGG+TGG - chr8.1:26699114-26699133 None:intergenic 45.0%
CCTTTGCCCGAGAATTACTT+CGG + chr8.1:26699114-26699133 MS.gene011796:CDS 45.0%
GACGAGGTGGCAATAGAATA+AGG - chr8.1:26699634-26699653 None:intergenic 45.0%
TAGTTGTGAGAATTGGGCCT+GGG - chr8.1:26699610-26699629 None:intergenic 45.0%
TGGCAACTAGTAGTTCTCCA+AGG + chr8.1:26699328-26699347 MS.gene011796:CDS 45.0%
TTCGGAAATGCATTGCAGGT+TGG + chr8.1:26699132-26699151 MS.gene011796:CDS 45.0%
! AAGCCTGTTGCTGTTAGAAG+TGG + chr8.1:26699384-26699403 MS.gene011796:CDS 45.0%
! AGCCTGTTGCTGTTAGAAGT+GGG + chr8.1:26699385-26699404 MS.gene011796:CDS 45.0%
! GGGGGTGCAAATAAAAGTGA+TGG + chr8.1:26699405-26699424 MS.gene011796:CDS 45.0%
! TCATGGTCTGAAATCTCACG+TGG + chr8.1:26698748-26698767 MS.gene011796:CDS 45.0%
!! CTTCTCTTGCTGCTTTCTGT+TGG + chr8.1:26699658-26699677 MS.gene011796:intron 45.0%
!! GAGGTGTTGGATTTGGTTTG+AGG - chr8.1:26698362-26698381 None:intergenic 45.0%
!! TTACTTGCTGAAGGAGCGTT+TGG + chr8.1:26699180-26699199 MS.gene011796:CDS 45.0%
AATGATGAGACGACCGGTGA+GGG - chr8.1:26698422-26698441 None:intergenic 50.0%
CAAGAGAAGAAAGGACGAGG+TGG - chr8.1:26699647-26699666 None:intergenic 50.0%
CACGAATCACAGCTTGCCAA+AGG - chr8.1:26699023-26699042 None:intergenic 50.0%
CAGCAAGAGAAGAAAGGACG+AGG - chr8.1:26699650-26699669 None:intergenic 50.0%
CCGAAGTAATTCTCGGGCAA+AGG - chr8.1:26699117-26699136 None:intergenic 50.0%
CTTTGGCAAGCTGTGATTCG+TGG + chr8.1:26699021-26699040 MS.gene011796:CDS 50.0%
GTAGTTGTGAGAATTGGGCC+TGG - chr8.1:26699611-26699630 None:intergenic 50.0%
! CCTGTTGCTGTTAGAAGTGG+GGG + chr8.1:26699387-26699406 MS.gene011796:CDS 50.0%
! GCCTGTTGCTGTTAGAAGTG+GGG + chr8.1:26699386-26699405 MS.gene011796:CDS 50.0%
! GGAAATGCATTGCAGGTTGG+TGG + chr8.1:26699135-26699154 MS.gene011796:CDS 50.0%
ATGATGAGACGACCGGTGAG+GGG - chr8.1:26698421-26698440 None:intergenic 55.0%
CAATGATGAGACGACCGGTG+AGG - chr8.1:26698423-26698442 None:intergenic 55.0%
CCCCCACTTCTAACAGCAAC+AGG - chr8.1:26699390-26699409 None:intergenic 55.0%
! GTTTTCTTCCCACCCCTCAC+CGG + chr8.1:26698406-26698425 MS.gene011796:CDS 55.0%
GCACTTGCCACACTAGGCCT+TGG - chr8.1:26699579-26699598 None:intergenic 60.0%
GGTGTGGCACTTGCCACACT+AGG - chr8.1:26699585-26699604 None:intergenic 60.0%
TGTGAGAATTGGGCCTGGGC+TGG - chr8.1:26699606-26699625 None:intergenic 60.0%
TTACTGTGCATGGGCGGACC+TGG + chr8.1:26699553-26699572 MS.gene011796:intron 60.0%
ATGAGACGACCGGTGAGGGG+TGG - chr8.1:26698418-26698437 None:intergenic 65.0%
GACCTGGCCAAGGCCTAGTG+TGG + chr8.1:26699569-26699588 MS.gene011796:intron 65.0%
TGAGACGACCGGTGAGGGGT+GGG - chr8.1:26698417-26698436 None:intergenic 65.0%
TGCCACACTAGGCCTTGGCC+AGG - chr8.1:26699574-26699593 None:intergenic 65.0%
!! GAATTGGGCCTGGGCTGGTG+TGG - chr8.1:26699601-26699620 None:intergenic 65.0%
GGCAAGTGCCACACCAGCCC+AGG + chr8.1:26699590-26699609 MS.gene011796:intron 70.0%
TGCATGGGCGGACCTGGCCA+AGG + chr8.1:26699559-26699578 MS.gene011796:intron 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 26698310 26699701 26698310 ID=MS.gene011796
chr8.1 mRNA 26698310 26699701 26698310 ID=MS.gene011796.t1;Parent=MS.gene011796
chr8.1 exon 26698310 26699541 26698310 ID=MS.gene011796.t1.exon1;Parent=MS.gene011796.t1
chr8.1 CDS 26698310 26699541 26698310 ID=cds.MS.gene011796.t1;Parent=MS.gene011796.t1
chr8.1 exon 26699689 26699701 26699689 ID=MS.gene011796.t1.exon2;Parent=MS.gene011796.t1
chr8.1 CDS 26699689 26699701 26699689 ID=cds.MS.gene011796.t1;Parent=MS.gene011796.t1
Gene Sequence

>MS.gene011796

ATGAACCAAAAAGGACTTCTCTTTCATAATCCCAAAAACCCAAAAGACACCTCAAACCAAATCCAACACCTCAAAAACACCCTTTCATCAACTCTTGTTTTCTTCCCACCCCTCACCGGTCGTCTCATCATTGAACATCAAGAAGAAGAAGACGGCGACAACAACAACACAACCTCATGTTGCATCCTTTGTAACAATCTAGGTGCGTTATTTATCCATGCAATAGCTGAAAACACAAGTGTCTCTGATATTCTTAAACCAAACTATGTTCCTTCCATTGTTCGCTCTTTTTTTCCGTTTAATAAAGTAAAAAACTACGAAGGCACATCAAAACCATTGCTTGCAGTTCAAGTTACTGAGCTTGTTGATGGCATTTTCATTGGCTTTTCAATCAATCATGTTGTTGTCGATGGTAAGTCGTTTTGGCATTTCATCAATTCATGGTCTGAAATCTCACGTGGTTTTGATCGACCATCGAAGCTTCCAACACTTAATCGTTGGTTTCTCGATGACTCAATTGAAATTCCTATAAAATTTCCATCCACAAAGGAGGAAAATAAAGAAAGTACTGAAGCTGAAACTGATGAAAACTCTATTCCACCTGAGAGGATTTTCCATTTCACAAAGGAGAAAATTACGAAACTGAAATCAAAAGCTAACGAAGAGGCCAATACAAACAAAATATCTTCTTTACAAGCACTTTTAACTCACCTTTGGCAAGCTGTGATTCGTGGTCAGAATATCGATCCAGAAGAAGAAATCATGTATTGTTTAGTCATTGATGTTAGAGGGAGAGTCATTCCACCTTTGCCCGAGAATTACTTCGGAAATGCATTGCAGGTTGGTGGTGTGAAAGTGAAAGCAAAGGAATTACTTGCTGAAGGAGCGTTTGGAAAAGTTGCTTTTGAGATGAACAAGATGATAGCTTCACATTGTGGTGATACTGTGAAGAGAAACTATGAAAGTTGGGTGAAAAATCCAAGGTTATTTCAAGGTAGGCTTAGTAGTGTTAAGGCTTTGGCAACTAGTAGTTCTCCAAGGTTTGATGTTTATGGTAACGACTTTGGTTGGGGTAAGCCTGTTGCTGTTAGAAGTGGGGGTGCAAATAAAAGTGATGGAACGATAACTATGTTTAATGGGGCAGAAGAAGGTAGTATTGATGTTGAAGTTTGTCTTTCTCATGATATTTTGGAGGCTATGGGAAATGACCCTCAATTTATACATATCTTTTGGTTAGTTTATGTTACTGTGCATGGGCGGACCTGGCCAAGGCCTAGTGTGGCAAGTGCCACACCAGCCCAGGCCCAATTCTCACAACTACCCTTATTCTATTGCCACCTCGTCCTTTCTTCTCTTGCTGCTTTCTGTTGGTGTAACAGCTTGTATAATTGA
Protein sequence

>MS.gene011796.t1

MNQKGLLFHNPKNPKDTSNQIQHLKNTLSSTLVFFPPLTGRLIIEHQEEEDGDNNNTTSCCILCNNLGALFIHAIAENTSVSDILKPNYVPSIVRSFFPFNKVKNYEGTSKPLLAVQVTELVDGIFIGFSINHVVVDGKSFWHFINSWSEISRGFDRPSKLPTLNRWFLDDSIEIPIKFPSTKEENKESTEAETDENSIPPERIFHFTKEKITKLKSKANEEANTNKISSLQALLTHLWQAVIRGQNIDPEEEIMYCLVIDVRGRVIPPLPENYFGNALQVGGVKVKAKELLAEGAFGKVAFEMNKMIASHCGDTVKRNYESWVKNPRLFQGRLSSVKALATSSSPRFDVYGNDFGWGKPVAVRSGGANKSDGTITMFNGAEEGSIDVEVCLSHDILEAMGNDPQFIHIFCLYN