AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066660.t1 MTR_4g022670 94.417 412 23 0 5 416 31 442 0.0 803
MS.gene066660.t1 MTR_4g022800 82.567 413 71 1 5 416 31 443 0.0 713
MS.gene066660.t1 MTR_4g022720 80.340 412 75 1 5 416 31 436 0.0 690
MS.gene066660.t1 MTR_7g026860 77.317 410 90 1 7 416 33 439 0.0 671
MS.gene066660.t1 MTR_4g022825 74.146 410 105 1 5 414 31 439 0.0 630
MS.gene066660.t1 MTR_4g022760 74.757 412 60 1 5 416 31 398 0.0 627
MS.gene066660.t1 MTR_3g088420 66.748 412 129 2 5 416 31 434 0.0 560
MS.gene066660.t1 MTR_3g026310 64.748 417 140 2 7 416 33 449 0.0 544
MS.gene066660.t1 MTR_8g041770 60.584 411 146 5 11 416 43 442 1.30e-173 493
MS.gene066660.t1 MTR_0190s0020 58.637 411 154 6 11 415 42 442 1.04e-170 485
MS.gene066660.t1 MTR_4g094842 57.209 430 154 9 3 413 28 446 3.45e-158 454
MS.gene066660.t1 MTR_4g094835 54.197 417 168 7 5 411 39 442 7.93e-150 432
MS.gene066660.t1 MTR_4g094832 56.579 380 138 7 46 411 60 426 1.68e-141 410
MS.gene066660.t1 MTR_4g022290 52.153 418 166 11 12 413 44 443 8.75e-137 399
MS.gene066660.t1 MTR_8g079710 52.696 408 166 9 12 406 43 436 1.05e-134 394
MS.gene066660.t1 MTR_4g094828 52.404 416 178 10 12 415 43 450 1.31e-134 394
MS.gene066660.t1 MTR_4g094838 50.000 418 164 8 10 411 40 428 5.38e-133 389
MS.gene066660.t1 MTR_6g016815 51.456 412 126 7 5 416 45 382 4.21e-130 380
MS.gene066660.t1 MTR_3g101900 40.141 426 226 12 6 415 36 448 3.40e-90 280
MS.gene066660.t1 MTR_3g101930 39.151 424 224 10 12 414 44 454 4.38e-87 272
MS.gene066660.t1 MTR_7g026770 67.172 198 37 1 115 312 132 301 1.44e-85 263
MS.gene066660.t1 MTR_7g026850 74.847 163 41 0 7 169 16 178 5.06e-85 257
MS.gene066660.t1 MTR_3g102020 39.089 417 227 9 12 412 43 448 1.19e-81 258
MS.gene066660.t1 MTR_3g101960 38.406 414 229 9 12 410 43 445 3.05e-79 252
MS.gene066660.t1 MTR_8g040780 57.407 216 88 2 199 410 12 227 1.72e-78 243
MS.gene066660.t1 MTR_3g101970 37.560 418 224 9 11 414 42 436 5.63e-78 248
MS.gene066660.t1 MTR_3g102010 37.232 419 237 9 12 415 44 451 2.31e-77 247
MS.gene066660.t1 MTR_8g040760 65.143 175 57 2 10 181 39 212 3.02e-73 229
MS.gene066660.t1 MTR_3g070170 31.982 444 234 13 12 411 47 466 1.55e-57 196
MS.gene066660.t1 MTR_4g015180 31.478 467 239 13 12 416 41 488 3.84e-56 192
MS.gene066660.t1 MTR_5g081960 30.193 467 242 13 12 415 41 486 1.69e-55 191
MS.gene066660.t1 MTR_6g034485 61.364 132 43 1 163 294 2 125 3.38e-49 163
MS.gene066660.t1 MTR_7g026800 54.286 175 48 5 182 354 16 160 1.13e-47 162
MS.gene066660.t1 MTR_3g102030 35.161 310 162 5 64 371 48 320 2.44e-47 165
MS.gene066660.t1 MTR_4g022780 53.107 177 51 5 182 356 17 163 6.65e-47 159
MS.gene066660.t1 MTR_6g034495 55.152 165 62 5 1 154 1 164 2.03e-46 157
MS.gene066660.t1 MTR_4g094825 50.888 169 75 4 10 173 104 269 1.30e-45 162
MS.gene066660.t1 MTR_4g094825 51.656 151 60 2 263 411 248 387 1.44e-44 159
MS.gene066660.t1 MTR_6g045553 28.935 432 268 10 11 411 48 471 1.70e-45 164
MS.gene066660.t1 MTR_7g026780 62.162 111 18 1 306 416 26 112 1.15e-39 138
MS.gene066660.t1 MTR_7g026830 78.571 84 18 0 57 140 15 98 5.61e-39 135
MS.gene066660.t1 MTR_7g026870 86.765 68 4 1 263 325 84 151 2.69e-32 120
MS.gene066660.t1 MTR_8g075610 26.385 379 260 11 29 402 47 411 2.64e-30 121
MS.gene066660.t1 MTR_4g007540 26.531 392 247 13 29 402 56 424 3.56e-29 118
MS.gene066660.t1 MTR_2g105330 26.053 380 263 10 29 402 56 423 6.35e-29 117
MS.gene066660.t1 MTR_2g064495 26.053 380 263 10 29 402 56 423 1.50e-28 116
MS.gene066660.t1 MTR_8g096530 28.029 421 231 17 27 410 57 442 1.53e-27 114
MS.gene066660.t1 MTR_3g098980 29.296 355 204 13 29 367 66 389 2.01e-27 113
MS.gene066660.t1 MTR_7g026880 90.385 52 5 0 364 415 2 53 9.83e-27 101
MS.gene066660.t1 MTR_3g026950 26.371 383 212 16 26 367 51 404 5.11e-23 100
MS.gene066660.t1 MTR_4g013050 27.147 361 201 13 24 367 48 363 6.79e-22 97.4
MS.gene066660.t1 MTR_7g027175 25.974 385 251 11 29 402 68 429 2.84e-20 92.8
MS.gene066660.t1 MTR_3g049150 25.823 395 260 13 26 400 52 433 1.78e-19 90.5
MS.gene066660.t1 MTR_1g081660 26.255 259 177 7 26 282 70 316 1.75e-18 87.4
MS.gene066660.t1 MTR_2g089670 25.130 386 242 13 12 366 60 429 2.76e-16 80.9
MS.gene066660.t1 MTR_7g114470 22.803 421 273 15 11 402 37 434 1.00e-15 79.3
MS.gene066660.t1 MTR_2g089755 24.208 442 281 18 4 406 43 469 1.08e-15 79.3
MS.gene066660.t1 MTR_2g089765 26.389 288 168 11 111 366 159 434 1.09e-15 79.3
MS.gene066660.t1 MTR_5g076700 22.770 426 276 16 11 407 36 437 1.16e-15 79.0
MS.gene066660.t1 MTR_1g040175 27.240 279 181 11 26 298 61 323 1.30e-15 79.0
MS.gene066660.t1 MTR_3g087640 24.396 414 273 11 24 406 62 466 1.35e-15 79.0
MS.gene066660.t1 MTR_2g089650 24.279 416 265 17 12 392 43 443 2.44e-15 78.2
MS.gene066660.t1 MTR_1g040195 25.183 409 251 20 11 402 35 405 2.47e-15 77.8
MS.gene066660.t1 MTR_6g015830 25.949 316 204 9 112 406 148 454 2.95e-15 77.8
MS.gene066660.t1 MTR_7g085930 23.117 385 259 9 17 373 47 422 5.33e-15 77.0
MS.gene066660.t1 MTR_7g014330 25.000 380 229 13 24 370 57 413 5.47e-15 77.0
MS.gene066660.t1 MTR_7g014250 24.603 378 233 12 24 370 57 413 5.88e-15 76.6
MS.gene066660.t1 MTR_6g015320 26.316 304 186 11 112 388 143 435 8.88e-15 76.3
MS.gene066660.t1 MTR_7g114520 23.546 361 244 14 18 367 53 392 9.26e-15 76.3
MS.gene066660.t1 MTR_4g127650 23.544 395 255 15 29 396 66 440 9.63e-15 76.3
MS.gene066660.t1 MTR_4g097090 23.481 362 246 9 29 372 65 413 1.17e-14 75.9
MS.gene066660.t1 MTR_7g084910 27.215 316 179 14 29 315 70 363 1.47e-14 75.5
MS.gene066660.t1 MTR_7g114550 22.581 434 266 17 11 407 36 436 1.53e-14 75.5
MS.gene066660.t1 MTR_7g084940 26.277 274 184 9 29 296 69 330 3.36e-14 74.3
MS.gene066660.t1 MTR_7g085990 23.650 389 252 13 17 373 43 418 3.72e-14 74.3
MS.gene066660.t1 MTR_7g086000 25.067 375 235 16 32 380 63 417 4.09e-14 74.3
MS.gene066660.t1 MTR_1g040320 24.931 361 200 14 18 367 51 351 4.11e-14 73.9
MS.gene066660.t1 MTR_7g015860 25.172 290 172 10 106 367 141 413 4.16e-14 74.3
MS.gene066660.t1 MTR_3g465990 25.000 304 195 11 29 318 68 352 8.53e-14 73.2
MS.gene066660.t1 MTR_1g040315 26.644 289 177 13 11 283 35 304 2.61e-13 71.6
MS.gene066660.t1 MTR_3g465930 26.190 294 190 11 29 313 69 344 5.31e-13 70.9
MS.gene066660.t1 MTR_3g465910 25.965 285 186 10 29 304 68 336 5.81e-13 70.9
MS.gene066660.t1 MTR_5g090830 23.896 385 238 13 17 367 49 412 1.06e-12 70.1
MS.gene066660.t1 MTR_6g053810 25.267 281 184 10 29 298 71 336 1.37e-12 69.7
MS.gene066660.t1 MTR_5g090810 23.377 385 240 13 17 367 49 412 1.39e-12 69.7
MS.gene066660.t1 MTR_3g112180 22.821 390 273 13 23 402 55 426 1.55e-11 66.2
MS.gene066660.t1 MTR_5g090880 21.868 439 283 15 17 415 49 467 3.69e-11 65.1
MS.gene066660.t1 MTR_7g014610 24.169 331 195 13 123 416 158 469 4.82e-11 64.7
MS.gene066660.t1 MTR_7g014200 23.947 380 227 14 29 370 69 424 7.98e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066660.t1 AT5G67150 39.903 411 225 11 12 411 43 442 1.85e-87 273
MS.gene066660.t1 AT3G50300 41.457 398 206 13 12 396 41 424 2.82e-84 265
MS.gene066660.t1 AT5G67160 38.971 408 227 10 12 415 43 432 1.35e-82 260
MS.gene066660.t1 AT3G50280 37.412 425 239 11 3 412 28 440 5.21e-81 256
MS.gene066660.t1 AT5G42830 38.369 417 233 11 12 415 44 449 2.17e-80 255
MS.gene066660.t1 AT3G50270 37.850 428 242 11 3 415 30 448 2.71e-78 249
MS.gene066660.t1 AT5G07850 40.347 404 220 11 22 416 63 454 3.62e-78 249
MS.gene066660.t1 AT5G07860 38.005 421 226 9 12 414 45 448 9.09e-78 248
MS.gene066660.t1 AT5G07870 38.462 429 229 12 12 416 45 462 1.82e-73 237
MS.gene066660.t1 AT5G38130 36.709 395 227 10 12 396 61 442 5.15e-70 228
MS.gene066660.t1 AT2G39980 33.111 450 245 14 12 416 43 481 1.66e-65 217
MS.gene066660.t1 AT5G01210 33.412 425 206 12 40 415 73 469 6.55e-63 210
MS.gene066660.t1 AT3G50280 37.812 320 179 7 101 412 3 310 1.40e-62 204
MS.gene066660.t1 AT3G50290 40.511 274 150 6 96 364 4 269 2.85e-52 176
MS.gene066660.t1 AT5G23940 31.116 421 260 13 11 411 38 448 5.23e-50 176
MS.gene066660.t1 AT5G48930 26.582 395 270 10 17 402 41 424 6.49e-32 126
MS.gene066660.t1 AT3G48720 28.367 349 216 13 29 367 61 385 8.49e-23 100
MS.gene066660.t1 AT5G41040 25.978 358 221 13 26 367 66 395 2.76e-21 95.9
MS.gene066660.t1 AT5G41040 25.978 358 221 13 26 367 82 411 3.45e-21 95.5
MS.gene066660.t1 AT2G19070 24.503 453 275 20 5 414 23 451 9.31e-20 91.3
MS.gene066660.t1 AT5G63560 26.136 352 220 12 27 367 59 381 2.59e-19 89.7
MS.gene066660.t1 AT3G29670 22.025 395 274 11 29 406 66 443 6.49e-17 82.8
MS.gene066660.t1 AT5G39080 25.366 410 251 16 29 404 65 453 8.16e-17 82.4
MS.gene066660.t1 AT3G29590 23.919 393 261 15 35 406 67 442 2.12e-16 81.3
MS.gene066660.t1 AT2G25150 24.932 365 229 13 26 370 64 403 4.57e-15 77.4
MS.gene066660.t1 AT3G29635 25.641 351 237 11 29 366 67 406 7.38e-15 76.6
MS.gene066660.t1 AT1G24430 24.259 371 235 13 29 388 62 397 1.44e-14 75.5
MS.gene066660.t1 AT5G07080 27.758 281 169 12 30 296 71 331 3.24e-14 74.7
MS.gene066660.t1 AT1G28680 22.969 357 224 14 32 369 65 389 6.87e-14 73.6
MS.gene066660.t1 AT3G29680 25.255 392 247 15 29 396 62 431 9.58e-14 73.2
MS.gene066660.t1 AT2G40230 25.926 351 234 11 29 371 59 391 2.71e-13 71.6
MS.gene066660.t1 AT5G61160 24.727 275 181 10 109 367 139 403 3.93e-13 71.2
MS.gene066660.t1 AT5G16410 24.798 371 233 15 15 367 77 419 9.73e-13 70.1
MS.gene066660.t1 AT5G47980 25.000 364 222 14 29 374 61 391 1.39e-12 69.3
MS.gene066660.t1 AT1G28680 23.355 304 199 11 32 323 65 346 1.79e-12 68.9
MS.gene066660.t1 AT1G24420 25.364 343 208 14 39 369 75 381 1.94e-12 68.9
MS.gene066660.t1 AT1G24420 25.364 343 208 14 39 369 75 381 1.94e-12 68.9
MS.gene066660.t1 AT1G78990 26.646 319 205 14 61 367 95 396 4.29e-12 68.2
MS.gene066660.t1 AT5G23970 26.276 392 230 20 26 397 51 403 7.06e-12 67.4
MS.gene066660.t1 AT1G32910 23.729 354 236 13 29 367 65 399 2.34e-11 65.9
MS.gene066660.t1 AT5G39050 22.727 330 212 12 90 389 126 442 3.44e-11 65.1

Find 95 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCATGGTTTGATGTTTA+TGG 0.113342 4.2:+80095523 MS.gene066660:CDS
CCGTTACCAGAAGATTATTT+TGG 0.221902 4.2:+80095292 MS.gene066660:CDS
AATGGATAAGAAATTATTAT+TGG 0.233186 4.2:-80095494 None:intergenic
ACTTGCTTCAACCAACAAAA+AGG 0.237428 4.2:+80094490 MS.gene066660:CDS
CGTAGTGGATATAAAATAAA+TGG 0.258298 4.2:+80095580 MS.gene066660:CDS
AGTGTCTCAGCCATTGGATT+TGG 0.273871 4.2:-80094544 None:intergenic
TAAGAAATCCAAGCTTTGTT+AGG 0.280992 4.2:+80095458 MS.gene066660:CDS
ATGTCTTCCTTACGAGGTTT+TGG 0.299283 4.2:+80095654 MS.gene066660:CDS
CATACGATTTCCTTTCACTA+TGG 0.304291 4.2:+80095000 MS.gene066660:CDS
GTGTCTCAGCCATTGGATTT+GGG 0.322989 4.2:-80094543 None:intergenic
GATGTTTATGGTAATGATTT+CGG 0.343194 4.2:+80095535 MS.gene066660:CDS
GAAACCAACGCTCTAGTGTT+GGG 0.360883 4.2:-80094958 None:intergenic
TATCCACTACGAACTGCTAT+AGG 0.366106 4.2:-80095568 None:intergenic
GAGTTTCCAAAATAATCTTC+TGG 0.384510 4.2:-80095298 None:intergenic
GGGAAGTGACCCACATTTCA+TGG 0.400457 4.2:+80095684 MS.gene066660:CDS
ATGGGAAGTTGAATACCATT+TGG 0.405055 4.2:-80094980 None:intergenic
TTCAACCATGTTGTCGTTGA+CGG 0.405415 4.2:+80094860 MS.gene066660:CDS
AAACCATGGAGAACTACTAA+TGG 0.406093 4.2:-80095512 None:intergenic
GGAATTGTAAATGTATTTGC+TGG 0.411545 4.2:+80095601 MS.gene066660:CDS
GCCGACAAGAGGTTGGAAAA+AGG 0.413365 4.2:-80094585 None:intergenic
GATTATATACTTCTTCTTGT+GGG 0.433778 4.2:-80095234 None:intergenic
TGTATAGTCAAAATGAAAGT+TGG 0.440205 4.2:+80095331 MS.gene066660:CDS
TATGGTAATGATTTCGGATG+GGG 0.444025 4.2:+80095541 MS.gene066660:CDS
AAGCGCTATTAACTCACCTT+TGG 0.445268 4.2:+80095182 MS.gene066660:CDS
GACGAAGGTGGATTATATAA+AGG 0.446121 4.2:+80095364 MS.gene066660:CDS
AATGAAAGTTGGTGAGTTGT+TGG 0.448624 4.2:+80095342 MS.gene066660:CDS
AACCTTGAAGCTGCATTAGT+TGG 0.453624 4.2:-80095716 None:intergenic
AAGAAATCCAAGCTTTGTTA+GGG 0.462160 4.2:+80095459 MS.gene066660:CDS
TACTTGTGTTGATGACATCT+TGG 0.474749 4.2:+80094703 MS.gene066660:CDS
TGGATTTGGGTTGCTACCAC+CGG 0.474893 4.2:-80094530 None:intergenic
TTGGTTGAAGCAAGTGAAAA+CGG 0.475450 4.2:-80094482 None:intergenic
TGTGGCATTTCATCAATTCA+TGG 0.479156 4.2:+80094891 MS.gene066660:CDS
AGATTATATACTTCTTCTTG+TGG 0.484423 4.2:-80095235 None:intergenic
CCAAAATAATCTTCTGGTAA+CGG 0.486289 4.2:-80095292 None:intergenic
AATCTAGTCGTGATAGGAGT+TGG 0.488221 4.2:+80095253 MS.gene066660:CDS
TGGGTCAAATTGTTTGCAAC+GGG 0.488474 4.2:-80095215 None:intergenic
CTAGCAACCCTAACAAAGCT+TGG 0.494801 4.2:-80095466 None:intergenic
GTATATAATCTAGTCGTGAT+AGG 0.497624 4.2:+80095247 MS.gene066660:CDS
ATAGAGAGTGTCTCAGCCAT+TGG 0.510672 4.2:-80094550 None:intergenic
GGAAACCAACGCTCTAGTGT+TGG 0.511281 4.2:-80094959 None:intergenic
TGAAGAATCACTATGAGTCT+TGG 0.513376 4.2:+80095434 MS.gene066660:CDS
CATAGTGAAAGGAAATCGTA+TGG 0.513721 4.2:-80094999 None:intergenic
GGGCCTTCTTTATCACCATC+CGG 0.514663 4.2:+80094511 MS.gene066660:CDS
CTAGAGCGTTGGTTTCCAAA+TGG 0.515960 4.2:+80094965 MS.gene066660:CDS
AGGCACGTCAAAACCATTGT+TGG 0.517049 4.2:+80094790 MS.gene066660:CDS
ACAATTAGAGGAACGTAGAC+CGG 0.517745 4.2:-80094728 None:intergenic
TTTATGGTAATGATTTCGGA+TGG 0.520125 4.2:+80095539 MS.gene066660:CDS
CGTAAGGAAGACATACTTGA+AGG 0.521339 4.2:-80095645 None:intergenic
GTGGCATTTCATCAATTCAT+GGG 0.527709 4.2:+80094892 MS.gene066660:CDS
TGTTGATGACATCTTGGGAC+CGG 0.532244 4.2:+80094709 MS.gene066660:CDS
TGTCGGCCGTCTTGAAATAA+CGG 0.534950 4.2:+80094601 MS.gene066660:CDS
GTGGGTCAAATTGTTTGCAA+CGG 0.538778 4.2:-80095216 None:intergenic
GTCAACGACAACATGGTTGA+AGG 0.541580 4.2:-80094858 None:intergenic
TATCCATTAGTAGTTCTCCA+TGG 0.544589 4.2:+80095509 MS.gene066660:CDS
GGTGAGTTGTTGGACGAAGG+TGG 0.550907 4.2:+80095352 MS.gene066660:CDS
ATTTCAAGACGGCCGACAAG+AGG 0.553153 4.2:-80094596 None:intergenic
ATTGCCTCCAAAACCTCGTA+AGG 0.558716 4.2:-80095661 None:intergenic
TTATGGTAATGATTTCGGAT+GGG 0.560500 4.2:+80095540 MS.gene066660:CDS
CAAGACGGCCGACAAGAGGT+TGG 0.562878 4.2:-80094592 None:intergenic
AACAAAATAGCCATTGTGAT+GGG 0.565478 4.2:+80095026 MS.gene066660:CDS
GTCACGACATCCATGAAATG+TGG 0.565781 4.2:-80095694 None:intergenic
ACTTGTGTTGATGACATCTT+GGG 0.567798 4.2:+80094704 MS.gene066660:CDS
GAAATCGAAAATCAATGAAG+AGG 0.568871 4.2:+80095132 MS.gene066660:CDS
AAACCAACGCTCTAGTGTTG+GGG 0.569521 4.2:-80094957 None:intergenic
GTATTTGCTGGAATAGAAGA+AGG 0.570455 4.2:+80095613 MS.gene066660:CDS
CAACAAAATAGCCATTGTGA+TGG 0.573773 4.2:+80095025 MS.gene066660:CDS
ACTTTCCGTCAACGACAACA+TGG 0.580850 4.2:-80094865 None:intergenic
TCAAGTATGTCTTCCTTACG+AGG 0.584114 4.2:+80095648 MS.gene066660:CDS
CAAGTAACGGAGCTAGTTGA+CGG 0.586570 4.2:+80094818 MS.gene066660:CDS
TGTGTTCCGTTATTTCAAGA+CGG 0.586751 4.2:-80094607 None:intergenic
ATTGTTGGCTGTTCAAGTAA+CGG 0.588367 4.2:+80094805 MS.gene066660:CDS
CCACCGGATGGTGATAAAGA+AGG 0.588719 4.2:-80094514 None:intergenic
GTTGGTGAGTTGTTGGACGA+AGG 0.590545 4.2:+80095349 MS.gene066660:CDS
CAAGTTAATCGCTTCACACA+CGG 0.591631 4.2:+80095402 MS.gene066660:CDS
GAAATATCACGTGGTTGTTG+TGG 0.597765 4.2:+80094917 MS.gene066660:CDS
GGTAACGGTGGAACAAACCT+TGG 0.603404 4.2:-80095277 None:intergenic
AACGGGTGACAAAGCACCAA+AGG 0.603630 4.2:-80095198 None:intergenic
AAGTGAAAACGGAGATCCCA+TGG 0.606554 4.2:-80094471 None:intergenic
TGGTAGCAACCCAAATCCAA+TGG 0.612872 4.2:+80094534 MS.gene066660:CDS
TAGGTTATTTCATTTCACAA+AGG 0.626286 4.2:+80095093 MS.gene066660:CDS
AACCAACGCTCTAGTGTTGG+GGG 0.632370 4.2:-80094956 None:intergenic
GTTACTTGAACAGCCAACAA+TGG 0.633211 4.2:-80094803 None:intergenic
ATAGTGAAAGGAAATCGTAT+GGG 0.637742 4.2:-80094998 None:intergenic
TTTGGGTTGCTACCACCGGA+TGG 0.640724 4.2:-80094526 None:intergenic
AAATAATCTTCTGGTAACGG+TGG 0.643941 4.2:-80095289 None:intergenic
TCACGACATCCATGAAATGT+GGG 0.644749 4.2:-80095693 None:intergenic
TCGTGATAGGAGTTGGACCA+AGG 0.645786 4.2:+80095260 MS.gene066660:CDS
TTACCATAAACATCAAACCA+TGG 0.659822 4.2:-80095526 None:intergenic
AAACCTATAGCAGTTCGTAG+TGG 0.661852 4.2:+80095565 MS.gene066660:CDS
TCTGTTATATGCAACAATGC+AGG 0.665996 4.2:+80094650 MS.gene066660:CDS
CCTTCTTTATCACCATCCGG+TGG 0.673821 4.2:+80094514 MS.gene066660:CDS
TGAATTGATGAAATGCCACA+CGG 0.679039 4.2:-80094888 None:intergenic
AAGTATTCTCTGCTGCAGCG+TGG 0.693165 4.2:-80094685 None:intergenic
TCATGGGCTGAAATATCACG+TGG 0.739984 4.2:+80094908 MS.gene066660:CDS
TCGTTGACGGAAAGTCCGTG+TGG 0.742237 4.2:+80094873 MS.gene066660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGGATAAGAAATTATTAT+TGG - chr4.2:80095497-80095516 None:intergenic 15.0%
!!! AAAAACTTTGTTTATCAAAT+AGG + chr4.2:80095074-80095093 MS.gene066660:CDS 15.0%
! AAAAATGAATCGACAATTAG+AGG - chr4.2:80094743-80094762 None:intergenic 25.0%
! AGATTATATACTTCTTCTTG+TGG - chr4.2:80095238-80095257 None:intergenic 25.0%
! GATTATATACTTCTTCTTGT+GGG - chr4.2:80095237-80095256 None:intergenic 25.0%
! TGTATAGTCAAAATGAAAGT+TGG + chr4.2:80095331-80095350 MS.gene066660:CDS 25.0%
!! ATAGTTTTTATCTCCGATAA+AGG - chr4.2:80094768-80094787 None:intergenic 25.0%
!! GATGTTTATGGTAATGATTT+CGG + chr4.2:80095535-80095554 MS.gene066660:CDS 25.0%
!! TAGGTTATTTCATTTCACAA+AGG + chr4.2:80095093-80095112 MS.gene066660:CDS 25.0%
!!! CGTAGTGGATATAAAATAAA+TGG + chr4.2:80095580-80095599 MS.gene066660:CDS 25.0%
!!! GATTCATTTTTTGCCTTTAT+CGG + chr4.2:80094752-80094771 MS.gene066660:CDS 25.0%
AACAAAATAGCCATTGTGAT+GGG + chr4.2:80095026-80095045 MS.gene066660:CDS 30.0%
ATAGTGAAAGGAAATCGTAT+GGG - chr4.2:80095001-80095020 None:intergenic 30.0%
ATCGGAGATAAAAACTATGA+AGG + chr4.2:80094770-80094789 MS.gene066660:CDS 30.0%
CCAAAATAATCTTCTGGTAA+CGG - chr4.2:80095295-80095314 None:intergenic 30.0%
GAAATCGAAAATCAATGAAG+AGG + chr4.2:80095132-80095151 MS.gene066660:CDS 30.0%
GAGTTTCCAAAATAATCTTC+TGG - chr4.2:80095301-80095320 None:intergenic 30.0%
GGAATTGTAAATGTATTTGC+TGG + chr4.2:80095601-80095620 MS.gene066660:CDS 30.0%
GTATATAATCTAGTCGTGAT+AGG + chr4.2:80095247-80095266 MS.gene066660:CDS 30.0%
TTACCATAAACATCAAACCA+TGG - chr4.2:80095529-80095548 None:intergenic 30.0%
! TTATGGTAATGATTTCGGAT+GGG + chr4.2:80095540-80095559 MS.gene066660:CDS 30.0%
! TTTATGGTAATGATTTCGGA+TGG + chr4.2:80095539-80095558 MS.gene066660:CDS 30.0%
!! AAGAAATCCAAGCTTTGTTA+GGG + chr4.2:80095459-80095478 MS.gene066660:CDS 30.0%
!! TAAGAAATCCAAGCTTTGTT+AGG + chr4.2:80095458-80095477 MS.gene066660:CDS 30.0%
!!! TTTTGTTGTTCCATAGTGAA+AGG - chr4.2:80095013-80095032 None:intergenic 30.0%
AAAAACTCAACCCATCACAA+TGG - chr4.2:80095039-80095058 None:intergenic 35.0%
AAACCATGGAGAACTACTAA+TGG - chr4.2:80095515-80095534 None:intergenic 35.0%
AAGAGGTTGGAAAAAGGAAA+GGG - chr4.2:80094582-80094601 None:intergenic 35.0%
ACTTGCTTCAACCAACAAAA+AGG + chr4.2:80094490-80094509 MS.gene066660:CDS 35.0%
ATGGGAAGTTGAATACCATT+TGG - chr4.2:80094983-80095002 None:intergenic 35.0%
CAACAAAATAGCCATTGTGA+TGG + chr4.2:80095025-80095044 MS.gene066660:CDS 35.0%
CATACGATTTCCTTTCACTA+TGG + chr4.2:80095000-80095019 MS.gene066660:CDS 35.0%
CATAGTGAAAGGAAATCGTA+TGG - chr4.2:80095002-80095021 None:intergenic 35.0%
CTTGCTTCAACCAACAAAAA+GGG + chr4.2:80094491-80094510 MS.gene066660:CDS 35.0%
GTGGCATTTCATCAATTCAT+GGG + chr4.2:80094892-80094911 MS.gene066660:CDS 35.0%
TATCCATTAGTAGTTCTCCA+TGG + chr4.2:80095509-80095528 MS.gene066660:CDS 35.0%
TCTCCATGGTTTGATGTTTA+TGG + chr4.2:80095523-80095542 MS.gene066660:CDS 35.0%
TCTGTTATATGCAACAATGC+AGG + chr4.2:80094650-80094669 MS.gene066660:CDS 35.0%
TGAAGAATCACTATGAGTCT+TGG + chr4.2:80095434-80095453 MS.gene066660:CDS 35.0%
TGAATTGATGAAATGCCACA+CGG - chr4.2:80094891-80094910 None:intergenic 35.0%
TGTGGCATTTCATCAATTCA+TGG + chr4.2:80094891-80094910 MS.gene066660:CDS 35.0%
TTGGTTGAAGCAAGTGAAAA+CGG - chr4.2:80094485-80094504 None:intergenic 35.0%
! ATAAAGAAGGCCCTTTTTGT+TGG - chr4.2:80094504-80094523 None:intergenic 35.0%
! ATTGTTGGCTGTTCAAGTAA+CGG + chr4.2:80094805-80094824 MS.gene066660:CDS 35.0%
! CCGTTACCAGAAGATTATTT+TGG + chr4.2:80095292-80095311 MS.gene066660:CDS 35.0%
! CTAGTTGACGGTATTTTCAT+TGG + chr4.2:80094830-80094849 MS.gene066660:CDS 35.0%
! GTATTTGCTGGAATAGAAGA+AGG + chr4.2:80095613-80095632 MS.gene066660:CDS 35.0%
! TATGGTAATGATTTCGGATG+GGG + chr4.2:80095541-80095560 MS.gene066660:CDS 35.0%
! TGTGTTCCGTTATTTCAAGA+CGG - chr4.2:80094610-80094629 None:intergenic 35.0%
!! AAATAATCTTCTGGTAACGG+TGG - chr4.2:80095292-80095311 None:intergenic 35.0%
!! AATGAAAGTTGGTGAGTTGT+TGG + chr4.2:80095342-80095361 MS.gene066660:CDS 35.0%
!! ACTTGTGTTGATGACATCTT+GGG + chr4.2:80094704-80094723 MS.gene066660:CDS 35.0%
!! GACGAAGGTGGATTATATAA+AGG + chr4.2:80095364-80095383 MS.gene066660:CDS 35.0%
!! TACTTGTGTTGATGACATCT+TGG + chr4.2:80094703-80094722 MS.gene066660:CDS 35.0%
AAACCTATAGCAGTTCGTAG+TGG + chr4.2:80095565-80095584 MS.gene066660:CDS 40.0%
AAGCGCTATTAACTCACCTT+TGG + chr4.2:80095182-80095201 MS.gene066660:CDS 40.0%
AATCTAGTCGTGATAGGAGT+TGG + chr4.2:80095253-80095272 MS.gene066660:CDS 40.0%
ACAATTAGAGGAACGTAGAC+CGG - chr4.2:80094731-80094750 None:intergenic 40.0%
CAAGAGGTTGGAAAAAGGAA+AGG - chr4.2:80094583-80094602 None:intergenic 40.0%
CAAGTTAATCGCTTCACACA+CGG + chr4.2:80095402-80095421 MS.gene066660:CDS 40.0%
CGTAAGGAAGACATACTTGA+AGG - chr4.2:80095648-80095667 None:intergenic 40.0%
GAAATATCACGTGGTTGTTG+TGG + chr4.2:80094917-80094936 MS.gene066660:CDS 40.0%
GTGGGTCAAATTGTTTGCAA+CGG - chr4.2:80095219-80095238 None:intergenic 40.0%
GTTACTTGAACAGCCAACAA+TGG - chr4.2:80094806-80094825 None:intergenic 40.0%
TATCCACTACGAACTGCTAT+AGG - chr4.2:80095571-80095590 None:intergenic 40.0%
TCAAGTATGTCTTCCTTACG+AGG + chr4.2:80095648-80095667 MS.gene066660:CDS 40.0%
TCACGACATCCATGAAATGT+GGG - chr4.2:80095696-80095715 None:intergenic 40.0%
TGGGTCAAATTGTTTGCAAC+GGG - chr4.2:80095218-80095237 None:intergenic 40.0%
TTCAACCATGTTGTCGTTGA+CGG + chr4.2:80094860-80094879 MS.gene066660:CDS 40.0%
! ATGTCTTCCTTACGAGGTTT+TGG + chr4.2:80095654-80095673 MS.gene066660:CDS 40.0%
! TCCTTTTTCCAACCTCTTGT+CGG + chr4.2:80094584-80094603 MS.gene066660:CDS 40.0%
!! GAGTTTTTCAGCGTCTAATG+AGG + chr4.2:80095051-80095070 MS.gene066660:CDS 40.0%
AAACCAACGCTCTAGTGTTG+GGG - chr4.2:80094960-80094979 None:intergenic 45.0%
ACTTTCCGTCAACGACAACA+TGG - chr4.2:80094868-80094887 None:intergenic 45.0%
AGGCACGTCAAAACCATTGT+TGG + chr4.2:80094790-80094809 MS.gene066660:CDS 45.0%
AGTGTCTCAGCCATTGGATT+TGG - chr4.2:80094547-80094566 None:intergenic 45.0%
ATTGCCTCCAAAACCTCGTA+AGG - chr4.2:80095664-80095683 None:intergenic 45.0%
CTAGCAACCCTAACAAAGCT+TGG - chr4.2:80095469-80095488 None:intergenic 45.0%
GAAACCAACGCTCTAGTGTT+GGG - chr4.2:80094961-80094980 None:intergenic 45.0%
GTCAACGACAACATGGTTGA+AGG - chr4.2:80094861-80094880 None:intergenic 45.0%
GTCACGACATCCATGAAATG+TGG - chr4.2:80095697-80095716 None:intergenic 45.0%
GTGTCTCAGCCATTGGATTT+GGG - chr4.2:80094546-80094565 None:intergenic 45.0%
TGGTAGCAACCCAAATCCAA+TGG + chr4.2:80094534-80094553 MS.gene066660:CDS 45.0%
TGTCGGCCGTCTTGAAATAA+CGG + chr4.2:80094601-80094620 MS.gene066660:CDS 45.0%
TTGGAAAAAGGAAAGGGCAG+AGG - chr4.2:80094576-80094595 None:intergenic 45.0%
! CAAGTAACGGAGCTAGTTGA+CGG + chr4.2:80094818-80094837 MS.gene066660:CDS 45.0%
! TCATGGGCTGAAATATCACG+TGG + chr4.2:80094908-80094927 MS.gene066660:CDS 45.0%
!! ATAGAGAGTGTCTCAGCCAT+TGG - chr4.2:80094553-80094572 None:intergenic 45.0%
!! CTAGAGCGTTGGTTTCCAAA+TGG + chr4.2:80094965-80094984 MS.gene066660:CDS 45.0%
!! TCTTCCTTACGAGGTTTTGG+AGG + chr4.2:80095657-80095676 MS.gene066660:CDS 45.0%
!! TGTTGATGACATCTTGGGAC+CGG + chr4.2:80094709-80094728 MS.gene066660:CDS 45.0%
!!! TACGAGGTTTTGGAGGCAAT+GGG + chr4.2:80095664-80095683 MS.gene066660:CDS 45.0%
!!! TTACGAGGTTTTGGAGGCAA+TGG + chr4.2:80095663-80095682 MS.gene066660:CDS 45.0%
AAGTATTCTCTGCTGCAGCG+TGG - chr4.2:80094688-80094707 None:intergenic 50.0%
ATTTCAAGACGGCCGACAAG+AGG - chr4.2:80094599-80094618 None:intergenic 50.0%
CCACCGGATGGTGATAAAGA+AGG - chr4.2:80094517-80094536 None:intergenic 50.0%
CCTTCTTTATCACCATCCGG+TGG + chr4.2:80094514-80094533 MS.gene066660:CDS 50.0%
GCCGACAAGAGGTTGGAAAA+AGG - chr4.2:80094588-80094607 None:intergenic 50.0%
GGAAACCAACGCTCTAGTGT+TGG - chr4.2:80094962-80094981 None:intergenic 50.0%
GGGAAGTGACCCACATTTCA+TGG + chr4.2:80095684-80095703 MS.gene066660:CDS 50.0%
GGGCCTTCTTTATCACCATC+CGG + chr4.2:80094511-80094530 MS.gene066660:CDS 50.0%
TCGTGATAGGAGTTGGACCA+AGG + chr4.2:80095260-80095279 MS.gene066660:CDS 50.0%
! AACCAACGCTCTAGTGTTGG+GGG - chr4.2:80094959-80094978 None:intergenic 50.0%
! AACGGGTGACAAAGCACCAA+AGG - chr4.2:80095201-80095220 None:intergenic 50.0%
! GGTAACGGTGGAACAAACCT+TGG - chr4.2:80095280-80095299 None:intergenic 50.0%
! GTTGGTGAGTTGTTGGACGA+AGG + chr4.2:80095349-80095368 MS.gene066660:CDS 50.0%
!! TGGATTTGGGTTGCTACCAC+CGG - chr4.2:80094533-80094552 None:intergenic 50.0%
AACCCCCAACACTAGAGCGT+TGG + chr4.2:80094954-80094973 MS.gene066660:CDS 55.0%
GGTGAGTTGTTGGACGAAGG+TGG + chr4.2:80095352-80095371 MS.gene066660:CDS 55.0%
TCGTTGACGGAAAGTCCGTG+TGG + chr4.2:80094873-80094892 MS.gene066660:CDS 55.0%
! ACGCTCTAGTGTTGGGGGTT+TGG - chr4.2:80094954-80094973 None:intergenic 55.0%
!! TTTGGGTTGCTACCACCGGA+TGG - chr4.2:80094529-80094548 None:intergenic 55.0%
CAAGACGGCCGACAAGAGGT+TGG - chr4.2:80094595-80094614 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 80094473 80095723 80094473 ID=MS.gene066660
chr4.2 mRNA 80094473 80095723 80094473 ID=MS.gene066660.t1;Parent=MS.gene066660
chr4.2 exon 80094473 80095723 80094473 ID=MS.gene066660.t1.exon1;Parent=MS.gene066660.t1
chr4.2 CDS 80094473 80095723 80094473 ID=cds.MS.gene066660.t1;Parent=MS.gene066660.t1
Gene Sequence

>MS.gene066660

ATGGGATCTCCGTTTTCACTTGCTTCAACCAACAAAAAGGGCCTTCTTTATCACCATCCGGTGGTAGCAACCCAAATCCAATGGCTGAGACACTCTCTATCCTCTGCCCTTTCCTTTTTCCAACCTCTTGTCGGCCGTCTTGAAATAACGGAACACAAAGACAATATTGTCTCGTGTTCTGTTATATGCAACAATGCAGGTGTACTCTTTATCCACGCTGCAGCAGAGAATACTTGTGTTGATGACATCTTGGGACCGGTCTACGTTCCTCTAATTGTCGATTCATTTTTTGCCTTTATCGGAGATAAAAACTATGAAGGCACGTCAAAACCATTGTTGGCTGTTCAAGTAACGGAGCTAGTTGACGGTATTTTCATTGGTTGCTCCTTCAACCATGTTGTCGTTGACGGAAAGTCCGTGTGGCATTTCATCAATTCATGGGCTGAAATATCACGTGGTTGTTGTGGTGATCAAATATCCAAACCCCCAACACTAGAGCGTTGGTTTCCAAATGGTATTCAACTTCCCATACGATTTCCTTTCACTATGGAACAACAAAATAGCCATTGTGATGGGTTGAGTTTTTCAGCGTCTAATGAGGAAAAACTTTGTTTATCAAATAGGTTATTTCATTTCACAAAGGAGAAAATCATTCAACTGAAATCGAAAATCAATGAAGAGGTTAGTACAATCAAAATATCATCTCTACAAGCGCTATTAACTCACCTTTGGTGCTTTGTCACCCGTTGCAAACAATTTGACCCACAAGAAGAAGTATATAATCTAGTCGTGATAGGAGTTGGACCAAGGTTTGTTCCACCGTTACCAGAAGATTATTTTGGAAACTCGATTATAAGTTGTATAGTCAAAATGAAAGTTGGTGAGTTGTTGGACGAAGGTGGATTATATAAAGGTGCTTGTGAGATGAACAAGTTAATCGCTTCACACACGGATGAGAAGTTGAAGAATCACTATGAGTCTTGGCTAAGAAATCCAAGCTTTGTTAGGGTTGCTAGCATGTCCAATAATAATTTCTTATCCATTAGTAGTTCTCCATGGTTTGATGTTTATGGTAATGATTTCGGATGGGGAAAACCTATAGCAGTTCGTAGTGGATATAAAATAAATGGAATTGTAAATGTATTTGCTGGAATAGAAGAAGGTAGTATTGACCTTCAAGTATGTCTTCCTTACGAGGTTTTGGAGGCAATGGGAAGTGACCCACATTTCATGGATGTCGTGACCAACTAA
Protein sequence

>MS.gene066660.t1

MGSPFSLASTNKKGLLYHHPVVATQIQWLRHSLSSALSFFQPLVGRLEITEHKDNIVSCSVICNNAGVLFIHAAAENTCVDDILGPVYVPLIVDSFFAFIGDKNYEGTSKPLLAVQVTELVDGIFIGCSFNHVVVDGKSVWHFINSWAEISRGCCGDQISKPPTLERWFPNGIQLPIRFPFTMEQQNSHCDGLSFSASNEEKLCLSNRLFHFTKEKIIQLKSKINEEVSTIKISSLQALLTHLWCFVTRCKQFDPQEEVYNLVVIGVGPRFVPPLPEDYFGNSIISCIVKMKVGELLDEGGLYKGACEMNKLIASHTDEKLKNHYESWLRNPSFVRVASMSNNNFLSISSSPWFDVYGNDFGWGKPIAVRSGYKINGIVNVFAGIEEGSIDLQVCLPYEVLEAMGSDPHFMDVVTN