AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90492

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90492.t1 MTR_4g094835 93.188 367 24 1 26 392 81 446 0.0 714
MS.gene90492.t1 MTR_4g094832 87.399 373 43 1 25 393 59 431 0.0 670
MS.gene90492.t1 MTR_4g094838 82.114 369 49 2 26 393 81 433 0.0 608
MS.gene90492.t1 MTR_4g094842 60.916 371 132 6 26 388 79 444 5.09e-162 462
MS.gene90492.t1 MTR_8g041770 60.109 366 134 5 26 388 81 437 1.32e-154 443
MS.gene90492.t1 MTR_0190s0020 58.311 367 141 5 26 388 80 438 3.96e-151 434
MS.gene90492.t1 MTR_4g022720 58.108 370 138 6 26 388 72 431 7.90e-146 421
MS.gene90492.t1 MTR_4g022825 56.267 375 142 7 26 388 72 436 3.93e-145 419
MS.gene90492.t1 MTR_8g079710 57.808 365 143 8 26 383 76 436 2.79e-140 407
MS.gene90492.t1 MTR_4g022800 56.233 377 141 7 26 388 72 438 3.83e-140 407
MS.gene90492.t1 MTR_4g022670 54.787 376 147 6 26 388 72 437 1.61e-139 405
MS.gene90492.t1 MTR_3g088420 55.827 369 146 7 26 388 72 429 2.11e-137 399
MS.gene90492.t1 MTR_3g026310 52.742 383 151 7 26 388 72 444 1.34e-135 395
MS.gene90492.t1 MTR_4g022290 55.285 369 147 8 26 384 77 437 1.52e-134 392
MS.gene90492.t1 MTR_4g094828 53.887 373 152 9 26 384 76 442 1.83e-134 392
MS.gene90492.t1 MTR_7g026860 53.989 376 147 7 26 388 72 434 7.53e-134 390
MS.gene90492.t1 MTR_4g022760 47.872 376 129 6 26 388 72 393 2.88e-111 332
MS.gene90492.t1 MTR_3g101900 42.775 346 188 5 48 388 104 444 5.24e-87 271
MS.gene90492.t1 MTR_3g101930 41.547 349 191 4 48 388 108 451 1.13e-86 270
MS.gene90492.t1 MTR_6g016815 42.697 356 127 10 38 388 94 377 2.38e-81 254
MS.gene90492.t1 MTR_3g102020 41.210 347 193 5 48 388 106 447 5.76e-81 256
MS.gene90492.t1 MTR_3g101970 40.816 343 186 5 48 388 106 433 1.58e-79 251
MS.gene90492.t1 MTR_8g040780 58.491 212 81 3 179 387 20 227 3.55e-79 244
MS.gene90492.t1 MTR_3g101960 41.040 346 193 5 48 387 105 445 8.84e-79 250
MS.gene90492.t1 MTR_3g102010 41.595 351 190 5 48 388 102 447 1.47e-78 249
MS.gene90492.t1 MTR_3g070170 35.696 381 208 6 45 392 94 470 2.17e-69 226
MS.gene90492.t1 MTR_4g094825 63.905 169 46 2 230 393 234 392 5.10e-68 220
MS.gene90492.t1 MTR_4g094825 70.229 131 39 0 25 155 139 269 7.95e-50 172
MS.gene90492.t1 MTR_5g081960 31.193 436 242 6 12 393 56 487 1.16e-64 214
MS.gene90492.t1 MTR_4g015180 33.496 409 212 7 36 388 79 483 2.19e-64 213
MS.gene90492.t1 MTR_6g045553 31.389 360 190 8 75 389 125 472 1.84e-47 168
MS.gene90492.t1 MTR_3g102030 31.624 351 198 9 3 346 5 320 2.74e-47 164
MS.gene90492.t1 MTR_6g034485 58.915 129 47 2 144 270 1 125 4.03e-46 154
MS.gene90492.t1 MTR_8g040760 56.028 141 57 2 26 165 78 214 6.28e-46 157
MS.gene90492.t1 MTR_7g026770 47.222 180 71 4 99 276 132 289 2.81e-43 153
MS.gene90492.t1 MTR_7g026850 58.929 112 42 1 26 137 55 162 2.21e-41 144
MS.gene90492.t1 MTR_6g034495 58.929 112 45 1 26 137 51 161 3.90e-41 143
MS.gene90492.t1 MTR_7g026830 65.060 83 29 0 40 122 14 96 3.09e-32 117
MS.gene90492.t1 MTR_4g007540 24.658 365 241 13 37 381 76 426 1.72e-26 110
MS.gene90492.t1 MTR_3g098980 29.577 355 210 16 40 375 87 420 2.63e-25 107
MS.gene90492.t1 MTR_8g075610 24.722 360 241 13 38 381 68 413 3.25e-25 106
MS.gene90492.t1 MTR_2g064495 23.034 356 241 11 37 373 76 417 1.93e-23 101
MS.gene90492.t1 MTR_2g105330 23.034 356 241 11 37 373 76 417 5.17e-23 100
MS.gene90492.t1 MTR_3g026950 25.373 402 234 15 22 377 56 437 1.41e-21 96.3
MS.gene90492.t1 MTR_7g026800 37.805 164 68 6 168 329 29 160 1.36e-20 89.0
MS.gene90492.t1 MTR_8g096530 26.765 340 211 13 22 341 61 382 4.39e-20 92.0
MS.gene90492.t1 MTR_4g022780 36.747 166 71 6 168 331 30 163 1.52e-19 85.9
MS.gene90492.t1 MTR_7g026780 43.519 108 35 2 281 388 26 107 2.11e-19 83.6
MS.gene90492.t1 MTR_1g053315 26.349 315 190 15 51 342 115 410 1.22e-18 87.8
MS.gene90492.t1 MTR_4g127650 22.438 361 230 10 47 373 96 440 1.22e-18 87.8
MS.gene90492.t1 MTR_3g049150 25.325 308 201 11 95 377 130 433 1.55e-18 87.4
MS.gene90492.t1 MTR_4g097090 22.678 366 244 11 37 373 85 440 2.21e-18 87.0
MS.gene90492.t1 MTR_6g015315 24.920 313 202 9 96 382 140 445 4.58e-18 85.9
MS.gene90492.t1 MTR_3g086020 25.450 389 254 16 14 393 55 416 3.42e-17 83.2
MS.gene90492.t1 MTR_8g079970 23.881 335 217 10 44 358 82 398 4.19e-17 82.8
MS.gene90492.t1 MTR_1g040195 24.642 349 233 12 47 379 71 405 4.49e-17 82.8
MS.gene90492.t1 MTR_4g013050 24.384 406 238 13 9 383 37 404 7.79e-17 82.0
MS.gene90492.t1 MTR_2g089765 28.483 323 176 16 95 376 159 467 1.05e-16 82.0
MS.gene90492.t1 MTR_1g081660 23.488 430 244 17 2 392 68 451 1.25e-16 81.6
MS.gene90492.t1 MTR_7g027175 24.928 349 229 14 47 379 98 429 2.19e-16 80.9
MS.gene90492.t1 MTR_4g055170 29.091 220 143 6 90 300 140 355 3.50e-16 80.5
MS.gene90492.t1 MTR_6g015320 25.974 308 198 9 96 379 143 444 4.44e-16 80.1
MS.gene90492.t1 MTR_3g028210 25.382 327 205 12 78 373 130 448 9.02e-16 79.0
MS.gene90492.t1 MTR_6g015850 25.836 329 208 12 78 381 132 449 6.04e-15 76.6
MS.gene90492.t1 MTR_3g049140 24.786 234 154 6 158 377 5 230 1.25e-14 73.6
MS.gene90492.t1 MTR_7g084940 24.691 324 210 11 34 341 87 392 2.46e-14 74.7
MS.gene90492.t1 MTR_3g071320 25.088 283 186 8 33 297 77 351 4.94e-14 73.6
MS.gene90492.t1 MTR_6g015815 24.556 338 204 12 78 381 132 452 5.84e-14 73.6
MS.gene90492.t1 MTR_8g012750 25.788 349 199 14 77 379 126 460 7.38e-14 73.2
MS.gene90492.t1 MTR_3g112180 26.400 250 143 10 47 274 96 326 1.31e-13 72.4
MS.gene90492.t1 MTR_5g084850 24.064 374 228 16 44 392 82 424 1.56e-13 72.0
MS.gene90492.t1 MTR_3g087640 27.052 329 196 16 83 379 146 462 1.72e-13 72.0
MS.gene90492.t1 MTR_7g014230 24.595 309 192 12 77 354 126 424 2.62e-13 71.6
MS.gene90492.t1 MTR_7g015860 23.975 317 188 10 95 376 147 445 7.38e-13 70.1
MS.gene90492.t1 MTR_2g089650 24.497 298 194 10 95 369 154 443 2.28e-12 68.6
MS.gene90492.t1 MTR_7g085990 25.410 366 233 14 45 376 83 442 2.44e-12 68.6
MS.gene90492.t1 MTR_7g026870 50.704 71 28 3 236 300 82 151 2.58e-12 64.7
MS.gene90492.t1 MTR_7g056087 22.689 357 229 14 45 375 88 423 3.77e-12 67.8
MS.gene90492.t1 MTR_7g084910 24.409 254 166 6 34 271 88 331 4.68e-12 67.4
MS.gene90492.t1 MTR_5g026320 23.000 400 254 14 19 387 72 448 8.04e-12 67.0
MS.gene90492.t1 MTR_5g084870 23.780 328 210 13 44 353 82 387 1.03e-11 66.6
MS.gene90492.t1 MTR_7g014610 25.962 312 176 14 106 373 157 457 1.83e-11 65.9
MS.gene90492.t1 MTR_7g026880 56.000 50 20 1 339 388 2 49 1.93e-11 59.3
MS.gene90492.t1 MTR_1g040175 23.446 354 237 14 47 381 94 432 1.93e-11 65.9
MS.gene90492.t1 MTR_1g040215 23.457 324 224 10 47 360 94 403 2.41e-11 65.5
MS.gene90492.t1 MTR_2g007880 23.720 371 240 15 42 388 95 446 5.07e-11 64.3
MS.gene90492.t1 MTR_7g014160 24.528 318 191 12 96 373 147 455 9.74e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90492.t1 AT5G67150 42.179 358 192 6 41 388 90 442 8.46e-97 296
MS.gene90492.t1 AT3G50300 42.105 361 197 7 38 388 85 443 5.56e-92 284
MS.gene90492.t1 AT3G50280 40.616 357 204 5 38 388 85 439 1.56e-88 275
MS.gene90492.t1 AT5G67160 40.503 358 196 7 37 387 80 427 1.32e-86 270
MS.gene90492.t1 AT3G50270 38.503 374 211 8 26 388 79 444 2.66e-86 269
MS.gene90492.t1 AT5G38130 41.159 345 187 10 38 373 105 442 1.77e-81 257
MS.gene90492.t1 AT5G07850 39.612 361 203 8 37 388 95 449 1.69e-77 247
MS.gene90492.t1 AT3G50280 41.158 311 176 4 83 388 1 309 2.37e-77 242
MS.gene90492.t1 AT5G01210 35.958 381 204 6 44 388 89 465 1.86e-75 242
MS.gene90492.t1 AT5G07860 38.965 367 198 10 34 388 93 445 2.83e-74 238
MS.gene90492.t1 AT5G42830 36.975 357 210 6 42 388 94 445 7.23e-73 234
MS.gene90492.t1 AT2G39980 33.079 393 208 5 45 388 90 476 3.53e-70 228
MS.gene90492.t1 AT5G07870 37.027 370 206 8 37 388 97 457 2.07e-67 221
MS.gene90492.t1 AT3G50290 42.164 268 145 4 80 339 4 269 9.40e-64 205
MS.gene90492.t1 AT5G23940 34.957 349 214 7 51 389 104 449 1.40e-56 193
MS.gene90492.t1 AT3G29590 27.795 331 200 12 78 383 126 442 1.23e-23 102
MS.gene90492.t1 AT5G48930 23.836 365 240 13 37 379 76 424 2.38e-23 101
MS.gene90492.t1 AT2G19070 23.757 362 243 12 47 381 88 443 7.74e-21 94.4
MS.gene90492.t1 AT3G29635 26.230 305 193 10 93 373 142 438 7.23e-20 91.3
MS.gene90492.t1 AT5G39090 28.618 304 186 16 93 376 140 432 7.40e-20 91.3
MS.gene90492.t1 AT5G39050 25.915 328 205 14 80 376 132 452 1.06e-19 90.9
MS.gene90492.t1 AT5G41040 25.714 350 223 13 44 375 112 442 1.68e-19 90.5
MS.gene90492.t1 AT5G41040 25.714 350 223 13 44 375 96 426 1.90e-19 90.1
MS.gene90492.t1 AT3G29670 24.339 378 253 12 17 373 68 433 3.90e-19 89.4
MS.gene90492.t1 AT2G40230 25.434 346 220 10 47 368 91 422 1.33e-18 87.4
MS.gene90492.t1 AT3G48720 25.326 383 243 17 23 381 64 427 1.96e-16 80.9
MS.gene90492.t1 AT5G57840 24.654 361 234 11 37 373 76 422 8.30e-16 79.3
MS.gene90492.t1 AT1G24430 25.770 357 229 12 22 365 64 397 1.07e-15 79.0
MS.gene90492.t1 AT1G28680 25.194 258 170 9 99 344 143 389 1.26e-15 78.6
MS.gene90492.t1 AT3G47170 25.676 222 145 4 97 302 153 370 5.40e-15 76.6
MS.gene90492.t1 AT1G03390 25.478 314 195 14 51 342 111 407 1.45e-14 75.5
MS.gene90492.t1 AT1G03940 25.080 311 194 13 94 376 150 449 1.68e-14 75.1
MS.gene90492.t1 AT5G07080 29.167 192 111 5 97 272 149 331 2.97e-14 74.3
MS.gene90492.t1 AT5G63560 23.864 352 219 15 44 373 88 412 3.73e-14 73.9
MS.gene90492.t1 AT5G61160 24.194 310 202 14 93 379 139 438 3.81e-14 73.9
MS.gene90492.t1 AT3G30280 24.728 368 245 17 43 392 83 436 6.39e-14 73.6
MS.gene90492.t1 AT1G65450 25.818 275 171 12 45 300 101 361 1.52e-13 72.4
MS.gene90492.t1 AT1G28680 24.519 208 143 5 99 297 143 345 2.71e-13 71.2
MS.gene90492.t1 AT5G23970 23.836 365 236 17 44 389 77 418 5.58e-13 70.5
MS.gene90492.t1 AT4G15390 22.616 367 251 15 41 388 83 435 6.92e-13 70.1
MS.gene90492.t1 AT4G15390 22.616 367 251 15 41 388 83 435 6.92e-13 70.1
MS.gene90492.t1 AT3G03480 22.552 337 227 12 39 361 95 411 2.21e-12 68.6
MS.gene90492.t1 AT5G16410 21.833 371 234 13 34 367 109 460 6.59e-11 64.3

Find 72 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGCTTGACGAGGATTATTT+TGG 0.141275 8.3:+23598871 MS.gene90492:CDS
AAGGAACATAGTTTGGTTTA+AGG 0.151696 8.3:-23598324 None:intergenic
TCTCCGAGGTTTGATGTTTA+TGG 0.199950 8.3:+23599099 MS.gene90492:CDS
GTAAAATGGAAAATTCTTTC+AGG 0.213962 8.3:-23598655 None:intergenic
CACATTCTGATGAGAAAATA+AGG 0.214956 8.3:+23598992 MS.gene90492:CDS
CGTTGAAATGAAGGGAGTTT+TGG 0.257934 8.3:-23598547 None:intergenic
GATTTGAACATTCTCTGTTT+TGG 0.269213 8.3:-23598722 None:intergenic
GGGAAATGATGCTGAGTTCT+TGG 0.295386 8.3:+23599266 MS.gene90492:CDS
GATGTTTATGGTAATGATTT+TGG 0.307637 8.3:+23599111 MS.gene90492:CDS
ACGTAATGAAAATCTTCTTC+TGG 0.318483 8.3:-23598814 None:intergenic
GTTGTTTGGGATTAATAGTT+TGG 0.327840 8.3:+23599047 MS.gene90492:CDS
ACTATAGAAGGAACATAGTT+TGG 0.352065 8.3:-23598331 None:intergenic
TGATGTGTTGTTGTTGTTTG+TGG 0.358976 8.3:-23598227 None:intergenic
TTTGAGATATTGGAGGCTTT+GGG 0.368507 8.3:+23599246 MS.gene90492:CDS
GGGAAGATTACTGTGTTTGC+AGG 0.373043 8.3:+23599183 MS.gene90492:CDS
ATTGGAAGTTCAACGTCGTT+TGG 0.373841 8.3:-23598577 None:intergenic
TTAGCTAATGGCATCTTCAT+AGG 0.378743 8.3:+23598433 MS.gene90492:CDS
TTTGTGATGGCAAGATGTTT+TGG 0.386713 8.3:+23598476 MS.gene90492:CDS
TTGAGAATGCCGAAGTTGTT+TGG 0.387603 8.3:+23599033 MS.gene90492:CDS
CATTAGCTAACTCCGTTACT+TGG 0.402418 8.3:-23598420 None:intergenic
TTGGAAGTTCAACGTCGTTT+GGG 0.408058 8.3:-23598576 None:intergenic
TTTGGAAATGCAGGGATCAT+TGG 0.408778 8.3:+23598889 MS.gene90492:CDS
TGAGAATGCCGAAGTTGTTT+GGG 0.411706 8.3:+23599034 MS.gene90492:CDS
TTTGGCAGGTGCTAATTCAT+TGG 0.414867 8.3:+23599065 MS.gene90492:CDS
TTTGGCATTTCGTCAATACT+TGG 0.430278 8.3:+23598494 MS.gene90492:CDS
TTTGGGATTAATAGTTTGGC+AGG 0.440696 8.3:+23599051 MS.gene90492:CDS
TGGGATTGGAAAATGTTCAT+TGG 0.444512 8.3:+23598948 MS.gene90492:CDS
AAGCGTTGTTCTCTCATGTA+TGG 0.446035 8.3:+23598761 MS.gene90492:CDS
TTTGTGAAAGGAAAGTGTAT+TGG 0.453790 8.3:-23598595 None:intergenic
GTAGTGTAACTATGAAAGTA+GGG 0.457732 8.3:+23598911 MS.gene90492:CDS
TTGAGATTATTGTTGTCAAT+TGG 0.459438 8.3:-23598001 None:intergenic
GGTAGTGTAACTATGAAAGT+AGG 0.460447 8.3:+23598910 MS.gene90492:CDS
GGAGTGCAAATAAAAGTAAT+GGG 0.465217 8.3:+23599163 MS.gene90492:CDS
CTCCCACTTCGAACCCCTAC+CGG 0.466626 8.3:-23599144 None:intergenic
GGGAACCAACGTTGAAATGA+AGG 0.474068 8.3:-23598556 None:intergenic
GGGAGTGCAAATAAAAGTAA+TGG 0.474744 8.3:+23599162 MS.gene90492:CDS
GTTTGTGGCTACGTTGTGTT+GGG 0.476915 8.3:-23598212 None:intergenic
AAACCGGTAGGGGTTCGAAG+TGG 0.479206 8.3:+23599141 MS.gene90492:CDS
CAAGTAACGGAGTTAGCTAA+TGG 0.482336 8.3:+23598421 MS.gene90492:CDS
ACCAAGGTTGTTGCAAATGA+TGG 0.487314 8.3:-23598254 None:intergenic
CAGTCAGATTACTTAGAACT+AGG 0.495624 8.3:-23599291 None:intergenic
GTTACTTGGACTGCTAGAAT+AGG 0.498322 8.3:-23598406 None:intergenic
TGTTTGTGGCTACGTTGTGT+TGG 0.512319 8.3:-23598213 None:intergenic
CTCAACCACGTTGTTTGTGA+TGG 0.514978 8.3:+23598463 MS.gene90492:CDS
GAGATTCTAGAATGTGGGAT+TGG 0.518222 8.3:+23598934 MS.gene90492:CDS
TAGGGGAGATTCTAGAATGT+GGG 0.520801 8.3:+23598929 MS.gene90492:CDS
TCCATCATTTGCAACAACCT+TGG 0.522468 8.3:+23598253 MS.gene90492:CDS
TATTGTTGTCAATTGGTGCT+TGG 0.527153 8.3:-23597994 None:intergenic
GTAGGGGAGATTCTAGAATG+TGG 0.529927 8.3:+23598928 MS.gene90492:CDS
ACATTCTGATGAGAAAATAA+GGG 0.533534 8.3:+23598993 MS.gene90492:CDS
AACCGGTAGGGGTTCGAAGT+GGG 0.542449 8.3:+23599142 MS.gene90492:CDS
TCAAGCGGAGGAATCATCCT+TGG 0.542679 8.3:-23598856 None:intergenic
AACTCCCTTCATTTCAACGT+TGG 0.545872 8.3:+23598551 MS.gene90492:CDS
CTTTCAGGTAAGTTCTGCGT+CGG 0.565322 8.3:-23598640 None:intergenic
GGAACCAACGTTGAAATGAA+GGG 0.576282 8.3:-23598555 None:intergenic
GTGTTTGCAGGAGCTGAAGA+AGG 0.577173 8.3:+23599195 MS.gene90492:CDS
CTATTAATCCCAAACAACTT+CGG 0.578183 8.3:-23599042 None:intergenic
TCTCTTCGTCCACGCAAAAG+CGG 0.578199 8.3:+23598279 MS.gene90492:CDS
CCAAAATAATCCTCGTCAAG+CGG 0.581464 8.3:-23598871 None:intergenic
TAAGGGATGATTATGAGTGT+TGG 0.592031 8.3:+23599010 MS.gene90492:CDS
TAGTTATAGGTGTTAGACCA+AGG 0.600032 8.3:+23598839 MS.gene90492:CDS
TATTCTAGCAGTCCAAGTAA+CGG 0.629197 8.3:+23598408 MS.gene90492:CDS
TGTGTTGGGTGATTATAAGA+CGG 0.629490 8.3:-23598198 None:intergenic
GATGATTCCTCCGCTTGACG+AGG 0.630798 8.3:+23598861 MS.gene90492:CDS
AAATAATCCTCGTCAAGCGG+AGG 0.636490 8.3:-23598868 None:intergenic
TGCTAGAATAGGTTGTGAAG+TGG 0.647856 8.3:-23598395 None:intergenic
ACTAGTTGAGATCACTGTGA+TGG 0.656954 8.3:-23597966 None:intergenic
TAGTGTAACTATGAAAGTAG+GGG 0.674189 8.3:+23598912 MS.gene90492:CDS
TTACCATAAACATCAAACCT+CGG 0.674952 8.3:-23599102 None:intergenic
TGGCTACGAGTAGTTCTCCG+AGG 0.676435 8.3:+23599085 MS.gene90492:CDS
CGTGGACGAAGAGAGCACCA+AGG 0.726493 8.3:-23598270 None:intergenic
TCTTGCCATCACAAACAACG+TGG 0.787958 8.3:-23598468 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAATTTTCCATTTTACAA+AGG + chr8.3:23598660-23598679 MS.gene90492:CDS 20.0%
!!! TTTCATTACGTTTTAGTTAT+AGG + chr8.3:23598826-23598845 MS.gene90492:CDS 20.0%
! AAAAATGAGTGAACTATAGA+AGG - chr8.3:23598346-23598365 None:intergenic 25.0%
! ACATTCTGATGAGAAAATAA+GGG + chr8.3:23598993-23599012 MS.gene90492:CDS 25.0%
! GTAAAATGGAAAATTCTTTC+AGG - chr8.3:23598658-23598677 None:intergenic 25.0%
! TTGAGATTATTGTTGTCAAT+TGG - chr8.3:23598004-23598023 None:intergenic 25.0%
! TTTGTTTCAAATGGTTGATT+TGG - chr8.3:23598138-23598157 None:intergenic 25.0%
!! AATAATCGATTTAACACCAT+AGG + chr8.3:23598042-23598061 MS.gene90492:intron 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr8.3:23599111-23599130 MS.gene90492:CDS 25.0%
!!! TTATGGTAATGATTTTGGTT+GGG + chr8.3:23599116-23599135 MS.gene90492:CDS 25.0%
!!! TTGTCTTTCTTTTGAGATAT+TGG + chr8.3:23599236-23599255 MS.gene90492:CDS 25.0%
!!! TTTATGGTAATGATTTTGGT+TGG + chr8.3:23599115-23599134 MS.gene90492:CDS 25.0%
ACGTAATGAAAATCTTCTTC+TGG - chr8.3:23598817-23598836 None:intergenic 30.0%
ACTATAGAAGGAACATAGTT+TGG - chr8.3:23598334-23598353 None:intergenic 30.0%
CACATTCTGATGAGAAAATA+AGG + chr8.3:23598992-23599011 MS.gene90492:CDS 30.0%
CTAGTTGAAACCATTCAAAA+AGG + chr8.3:23598079-23598098 MS.gene90492:intron 30.0%
CTATTAATCCCAAACAACTT+CGG - chr8.3:23599045-23599064 None:intergenic 30.0%
GGAGTGCAAATAAAAGTAAT+GGG + chr8.3:23599163-23599182 MS.gene90492:CDS 30.0%
GTAGTGTAACTATGAAAGTA+GGG + chr8.3:23598911-23598930 MS.gene90492:CDS 30.0%
TAGTGTAACTATGAAAGTAG+GGG + chr8.3:23598912-23598931 MS.gene90492:CDS 30.0%
TTACCATAAACATCAAACCT+CGG - chr8.3:23599105-23599124 None:intergenic 30.0%
! AAGGAACATAGTTTGGTTTA+AGG - chr8.3:23598327-23598346 None:intergenic 30.0%
! CGATTTTCTCCTTTGTAAAA+TGG - chr8.3:23598672-23598691 None:intergenic 30.0%
! CTTTTTGAATGGTTTCAACT+AGG - chr8.3:23598081-23598100 None:intergenic 30.0%
! GATTTGAACATTCTCTGTTT+TGG - chr8.3:23598725-23598744 None:intergenic 30.0%
! GTTGTTTGGGATTAATAGTT+TGG + chr8.3:23599047-23599066 MS.gene90492:CDS 30.0%
! TTTGTGAAAGGAAAGTGTAT+TGG - chr8.3:23598598-23598617 None:intergenic 30.0%
! TTTTACAAAGGAGAAAATCG+CGG + chr8.3:23598672-23598691 MS.gene90492:CDS 30.0%
!! AGTTTTTAACTCCGTTAAGT+GGG - chr8.3:23598369-23598388 None:intergenic 30.0%
!! TAGTTTTTAACTCCGTTAAG+TGG - chr8.3:23598370-23598389 None:intergenic 30.0%
!! TGGAAAGTGTTTGTTTCAAA+TGG - chr8.3:23598147-23598166 None:intergenic 30.0%
!!! AGGATTATTTTGGAAATGCA+GGG + chr8.3:23598881-23598900 MS.gene90492:CDS 30.0%
!!! ATTTGGTTTTGGTTTGCAAT+GGG - chr8.3:23598121-23598140 None:intergenic 30.0%
!!! TATGGTAATGATTTTGGTTG+GGG + chr8.3:23599117-23599136 MS.gene90492:CDS 30.0%
!!! TCAAATGGTTGATTTGGTTT+TGG - chr8.3:23598132-23598151 None:intergenic 30.0%
!!! TCTTTCTTTTGAGATATTGG+AGG + chr8.3:23599239-23599258 MS.gene90492:CDS 30.0%
ATTATAAGACGGCCTGTTAA+GGG - chr8.3:23598190-23598209 None:intergenic 35.0%
GGTAGTGTAACTATGAAAGT+AGG + chr8.3:23598910-23598929 MS.gene90492:CDS 35.0%
TAAGGGATGATTATGAGTGT+TGG + chr8.3:23599010-23599029 MS.gene90492:CDS 35.0%
TAGTTATAGGTGTTAGACCA+AGG + chr8.3:23598839-23598858 MS.gene90492:CDS 35.0%
TATTCTAGCAGTCCAAGTAA+CGG + chr8.3:23598408-23598427 MS.gene90492:CDS 35.0%
TGGGATTGGAAAATGTTCAT+TGG + chr8.3:23598948-23598967 MS.gene90492:CDS 35.0%
TGGTTTCAACTAGGAGAAAT+TGG - chr8.3:23598072-23598091 None:intergenic 35.0%
TGTGTTGGGTGATTATAAGA+CGG - chr8.3:23598201-23598220 None:intergenic 35.0%
TTAGCTAATGGCATCTTCAT+AGG + chr8.3:23598433-23598452 MS.gene90492:CDS 35.0%
TTTGGCATTTCGTCAATACT+TGG + chr8.3:23598494-23598513 MS.gene90492:CDS 35.0%
! CGGTTTTCTACATTGTGATT+CGG - chr8.3:23598623-23598642 None:intergenic 35.0%
! GAAAAGAAGGCCTTTTTGAA+TGG - chr8.3:23598092-23598111 None:intergenic 35.0%
! GGGAGTGCAAATAAAAGTAA+TGG + chr8.3:23599162-23599181 MS.gene90492:CDS 35.0%
! TATTGTTGTCAATTGGTGCT+TGG - chr8.3:23597997-23598016 None:intergenic 35.0%
! TTTGAGATATTGGAGGCTTT+GGG + chr8.3:23599246-23599265 MS.gene90492:CDS 35.0%
! TTTGGGATTAATAGTTTGGC+AGG + chr8.3:23599051-23599070 MS.gene90492:CDS 35.0%
! TTTGTGATGGCAAGATGTTT+TGG + chr8.3:23598476-23598495 MS.gene90492:CDS 35.0%
!! CACTCATTTTTCCCACTTAA+CGG + chr8.3:23598355-23598374 MS.gene90492:CDS 35.0%
!! TGATGTGTTGTTGTTGTTTG+TGG - chr8.3:23598230-23598249 None:intergenic 35.0%
!!! ATGGTAATGATTTTGGTTGG+GGG + chr8.3:23599118-23599137 MS.gene90492:CDS 35.0%
!!! GAGGATTATTTTGGAAATGC+AGG + chr8.3:23598880-23598899 MS.gene90492:CDS 35.0%
!!! GATTTGGTTTTGGTTTGCAA+TGG - chr8.3:23598122-23598141 None:intergenic 35.0%
!!! TGTGATTCGGTTTTTGTGAA+AGG - chr8.3:23598610-23598629 None:intergenic 35.0%
!!! TTTTGAGATATTGGAGGCTT+TGG + chr8.3:23599245-23599264 MS.gene90492:CDS 35.0%
AACTCCCTTCATTTCAACGT+TGG + chr8.3:23598551-23598570 MS.gene90492:CDS 40.0%
ACCAAGGTTGTTGCAAATGA+TGG - chr8.3:23598257-23598276 None:intergenic 40.0%
ACTAGTTGAGATCACTGTGA+TGG - chr8.3:23597969-23597988 None:intergenic 40.0%
AGGAGAAATTGGAGATCCTA+TGG - chr8.3:23598061-23598080 None:intergenic 40.0%
ATTGGAAGTTCAACGTCGTT+TGG - chr8.3:23598580-23598599 None:intergenic 40.0%
CATTAGCTAACTCCGTTACT+TGG - chr8.3:23598423-23598442 None:intergenic 40.0%
CCAAAATAATCCTCGTCAAG+CGG - chr8.3:23598874-23598893 None:intergenic 40.0%
GATTATAAGACGGCCTGTTA+AGG - chr8.3:23598191-23598210 None:intergenic 40.0%
GGAACCAACGTTGAAATGAA+GGG - chr8.3:23598558-23598577 None:intergenic 40.0%
GTTACTTGGACTGCTAGAAT+AGG - chr8.3:23598409-23598428 None:intergenic 40.0%
TCCATCATTTGCAACAACCT+TGG + chr8.3:23598253-23598272 MS.gene90492:CDS 40.0%
TCTCCGAGGTTTGATGTTTA+TGG + chr8.3:23599099-23599118 MS.gene90492:CDS 40.0%
TGAGAATGCCGAAGTTGTTT+GGG + chr8.3:23599034-23599053 MS.gene90492:CDS 40.0%
TGCTAGAATAGGTTGTGAAG+TGG - chr8.3:23598398-23598417 None:intergenic 40.0%
TTGAGAATGCCGAAGTTGTT+TGG + chr8.3:23599033-23599052 MS.gene90492:CDS 40.0%
TTGGAAGTTCAACGTCGTTT+GGG - chr8.3:23598579-23598598 None:intergenic 40.0%
TTTGGAAATGCAGGGATCAT+TGG + chr8.3:23598889-23598908 MS.gene90492:CDS 40.0%
TTTGGCAGGTGCTAATTCAT+TGG + chr8.3:23599065-23599084 MS.gene90492:CDS 40.0%
! AAGCGTTGTTCTCTCATGTA+TGG + chr8.3:23598761-23598780 MS.gene90492:CDS 40.0%
! CAAGTAACGGAGTTAGCTAA+TGG + chr8.3:23598421-23598440 MS.gene90492:CDS 40.0%
! CGTTGAAATGAAGGGAGTTT+TGG - chr8.3:23598550-23598569 None:intergenic 40.0%
! TCCTATTTTCCACCCTTAAC+AGG + chr8.3:23598175-23598194 MS.gene90492:intron 40.0%
!! AAGGGAGTTTTGGTATACTC+TGG - chr8.3:23598540-23598559 None:intergenic 40.0%
!! AGGGAGTTTTGGTATACTCT+GGG - chr8.3:23598539-23598558 None:intergenic 40.0%
!! CAATGGGTTGATGGAAAAGA+AGG - chr8.3:23598105-23598124 None:intergenic 40.0%
!! GAGATTCTAGAATGTGGGAT+TGG + chr8.3:23598934-23598953 MS.gene90492:CDS 40.0%
!! TAGGGGAGATTCTAGAATGT+GGG + chr8.3:23598929-23598948 MS.gene90492:CDS 40.0%
!! TGGAAAATAGGAGAGTGTTG+TGG - chr8.3:23598167-23598186 None:intergenic 40.0%
!! TTTGGTTTGCAATGGGTTGA+TGG - chr8.3:23598114-23598133 None:intergenic 40.0%
AAATAATCCTCGTCAAGCGG+AGG - chr8.3:23598871-23598890 None:intergenic 45.0%
CTCAACCACGTTGTTTGTGA+TGG + chr8.3:23598463-23598482 MS.gene90492:CDS 45.0%
GCCTGTTAAGGGTGGAAAAT+AGG - chr8.3:23598179-23598198 None:intergenic 45.0%
GGGAAATGATGCTGAGTTCT+TGG + chr8.3:23599266-23599285 MS.gene90492:CDS 45.0%
GGGAACCAACGTTGAAATGA+AGG - chr8.3:23598559-23598578 None:intergenic 45.0%
GGGAAGATTACTGTGTTTGC+AGG + chr8.3:23599183-23599202 MS.gene90492:CDS 45.0%
GTTTGTGGCTACGTTGTGTT+GGG - chr8.3:23598215-23598234 None:intergenic 45.0%
TCTTGCCATCACAAACAACG+TGG - chr8.3:23598471-23598490 None:intergenic 45.0%
TGTTTGTGGCTACGTTGTGT+TGG - chr8.3:23598216-23598235 None:intergenic 45.0%
! CCGCTTGACGAGGATTATTT+TGG + chr8.3:23598871-23598890 MS.gene90492:CDS 45.0%
! CTTTCAGGTAAGTTCTGCGT+CGG - chr8.3:23598643-23598662 None:intergenic 45.0%
!! GTAGGGGAGATTCTAGAATG+TGG + chr8.3:23598928-23598947 MS.gene90492:CDS 45.0%
!! TTGTGTTTTCCGCTTTTGCG+TGG - chr8.3:23598291-23598310 None:intergenic 45.0%
!!! TGATTTTGGTTGGGGGAAAC+CGG + chr8.3:23599125-23599144 MS.gene90492:CDS 45.0%
ATAAGACGGCCTGTTAAGGG+TGG - chr8.3:23598187-23598206 None:intergenic 50.0%
GTGTTTGCAGGAGCTGAAGA+AGG + chr8.3:23599195-23599214 MS.gene90492:CDS 50.0%
TCAAGCGGAGGAATCATCCT+TGG - chr8.3:23598859-23598878 None:intergenic 50.0%
TCTCTTCGTCCACGCAAAAG+CGG + chr8.3:23598279-23598298 MS.gene90492:CDS 50.0%
AAACCGGTAGGGGTTCGAAG+TGG + chr8.3:23599141-23599160 MS.gene90492:CDS 55.0%
AACCGGTAGGGGTTCGAAGT+GGG + chr8.3:23599142-23599161 MS.gene90492:CDS 55.0%
GATGATTCCTCCGCTTGACG+AGG + chr8.3:23598861-23598880 MS.gene90492:CDS 55.0%
TGGCTACGAGTAGTTCTCCG+AGG + chr8.3:23599085-23599104 MS.gene90492:CDS 55.0%
TTGGTTGGGGGAAACCGGTA+GGG + chr8.3:23599130-23599149 MS.gene90492:CDS 55.0%
TTTGGTTGGGGGAAACCGGT+AGG + chr8.3:23599129-23599148 MS.gene90492:CDS 55.0%
CTCCCACTTCGAACCCCTAC+CGG - chr8.3:23599147-23599166 None:intergenic 60.0%
TGGTTGGGGGAAACCGGTAG+GGG + chr8.3:23599131-23599150 MS.gene90492:CDS 60.0%
!! CGTGGACGAAGAGAGCACCA+AGG - chr8.3:23598273-23598292 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 23597959 23599305 23597959 ID=MS.gene90492
chr8.3 mRNA 23597959 23599305 23597959 ID=MS.gene90492.t1;Parent=MS.gene90492
chr8.3 exon 23597959 23598031 23597959 ID=MS.gene90492.t1.exon1;Parent=MS.gene90492.t1
chr8.3 CDS 23597959 23598031 23597959 ID=cds.MS.gene90492.t1;Parent=MS.gene90492.t1
chr8.3 exon 23598197 23599305 23598197 ID=MS.gene90492.t1.exon2;Parent=MS.gene90492.t1
chr8.3 CDS 23598197 23599305 23598197 ID=cds.MS.gene90492.t1;Parent=MS.gene90492.t1
Gene Sequence

>MS.gene90492

ATGGATTCCATCACAGTGATCTCAACTAGTACAATCCAAGCACCAATTGACAACAATAATCTCAACTCAAGAAGTGATTATCAAATAATCGATTTAACACCATAGGATCTCCAATTTCTCCTAGTTGAAACCATTCAAAAAGGCCTTCTTTTCCATCAACCCATTGCAAACCAAAACCAAATCAACCATTTGAAACAAACACTTTCCACAACACTCTCCTATTTTCCACCCTTAACAGGCCGTCTTATAATCACCCAACACAACGTAGCCACAAACAACAACAACACATCATGTTCCATCATTTGCAACAACCTTGGTGCTCTCTTCGTCCACGCAAAAGCGGAAAACACAACTGTATCAGACATCCTTAAACCAAACTATGTTCCTTCTATAGTTCACTCATTTTTCCCACTTAACGGAGTTAAAAACTACGAAGCCACTTCACAACCTATTCTAGCAGTCCAAGTAACGGAGTTAGCTAATGGCATCTTCATAGGTTTGACTCTCAACCACGTTGTTTGTGATGGCAAGATGTTTTGGCATTTCGTCAATACTTGGTCACAAATCTCAAAAAACTCCCAGAGTATACCAAAACTCCCTTCATTTCAACGTTGGTTCCCAAACGACGTTGAACTTCCAATACACTTTCCTTTCACAAAAACCGAATCACAATGTAGAAAACCGACGCAGAACTTACCTGAAAGAATTTTCCATTTTACAAAGGAGAAAATCGCGGAACTGAAATCAAAAGCAAATGAAGAAACCAAAACAGAGAATGTTCAAATCAAAATATCTTCGCTTCAAGCGTTGTTCTCTCATGTATGGCGATGTGTTGTTCGAAGCAAAAAACTCGATCCAGAAGAAGATTTTCATTACGTTTTAGTTATAGGTGTTAGACCAAGGATGATTCCTCCGCTTGACGAGGATTATTTTGGAAATGCAGGGATCATTGGTAGTGTAACTATGAAAGTAGGGGAGATTCTAGAATGTGGGATTGGAAAATGTTCATTGGAGATGAACAAGATGATTATGTCACATTCTGATGAGAAAATAAGGGATGATTATGAGTGTTGGTTGAGAATGCCGAAGTTGTTTGGGATTAATAGTTTGGCAGGTGCTAATTCATTGGCTACGAGTAGTTCTCCGAGGTTTGATGTTTATGGTAATGATTTTGGTTGGGGGAAACCGGTAGGGGTTCGAAGTGGGAGTGCAAATAAAAGTAATGGGAAGATTACTGTGTTTGCAGGAGCTGAAGAAGGTAGCATTGATATTGAAGTTTGTCTTTCTTTTGAGATATTGGAGGCTTTGGGAAATGATGCTGAGTTCTTGGTTCCTAGTTCTAAGTAA
Protein sequence

>MS.gene90492.t1

MDSITVISTSTIQAPIDNNNLNSRSRLIITQHNVATNNNNTSCSIICNNLGALFVHAKAENTTVSDILKPNYVPSIVHSFFPLNGVKNYEATSQPILAVQVTELANGIFIGLTLNHVVCDGKMFWHFVNTWSQISKNSQSIPKLPSFQRWFPNDVELPIHFPFTKTESQCRKPTQNLPERIFHFTKEKIAELKSKANEETKTENVQIKISSLQALFSHVWRCVVRSKKLDPEEDFHYVLVIGVRPRMIPPLDEDYFGNAGIIGSVTMKVGEILECGIGKCSLEMNKMIMSHSDEKIRDDYECWLRMPKLFGINSLAGANSLATSSSPRFDVYGNDFGWGKPVGVRSGSANKSNGKITVFAGAEEGSIDIEVCLSFEILEALGNDAEFLVPSSK