AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016477

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016477.t1 MTR_8g041770 87.444 446 51 1 1 446 1 441 0.0 807
MS.gene016477.t1 MTR_0190s0020 71.141 447 123 3 1 446 1 442 0.0 653
MS.gene016477.t1 MTR_4g022825 61.333 450 158 6 1 445 1 439 0.0 559
MS.gene016477.t1 MTR_3g088420 61.830 448 154 6 1 446 1 433 0.0 558
MS.gene016477.t1 MTR_4g022670 60.398 452 162 6 1 446 1 441 0.0 556
MS.gene016477.t1 MTR_4g022800 60.044 453 163 6 1 446 1 442 0.0 544
MS.gene016477.t1 MTR_4g022720 59.911 449 163 6 1 446 1 435 0.0 541
MS.gene016477.t1 MTR_3g026310 58.824 459 165 6 1 446 1 448 0.0 535
MS.gene016477.t1 MTR_7g026860 58.758 451 168 6 1 446 1 438 0.0 528
MS.gene016477.t1 MTR_4g094835 56.858 452 175 8 1 442 1 442 0.0 515
MS.gene016477.t1 MTR_4g094842 56.071 453 183 6 1 444 1 446 2.91e-176 501
MS.gene016477.t1 MTR_4g022760 53.437 451 151 5 1 446 1 397 2.61e-165 471
MS.gene016477.t1 MTR_4g094838 53.761 452 172 9 4 442 1 428 4.93e-162 464
MS.gene016477.t1 MTR_4g094828 52.516 457 199 9 1 446 1 450 1.27e-156 451
MS.gene016477.t1 MTR_4g094832 61.690 355 123 5 93 442 80 426 6.46e-156 448
MS.gene016477.t1 MTR_4g022290 51.641 457 194 8 1 444 1 443 5.17e-153 441
MS.gene016477.t1 MTR_8g079710 52.116 449 190 10 1 437 1 436 1.87e-149 432
MS.gene016477.t1 MTR_6g016815 48.667 450 144 10 1 446 15 381 5.52e-130 380
MS.gene016477.t1 MTR_3g101900 38.600 443 238 11 22 446 22 448 4.45e-95 294
MS.gene016477.t1 MTR_3g102020 38.549 441 247 10 19 443 16 448 3.58e-93 289
MS.gene016477.t1 MTR_3g101930 37.500 440 238 9 30 446 30 455 8.32e-91 283
MS.gene016477.t1 MTR_3g102010 39.070 430 241 9 30 446 30 451 1.01e-89 280
MS.gene016477.t1 MTR_8g040760 63.256 215 75 2 1 214 1 212 1.86e-89 271
MS.gene016477.t1 MTR_3g101970 38.028 426 236 9 30 445 29 436 9.66e-89 277
MS.gene016477.t1 MTR_8g040780 60.465 215 82 2 230 441 13 227 2.33e-86 264
MS.gene016477.t1 MTR_3g101960 38.353 425 241 10 30 441 29 445 6.88e-86 270
MS.gene016477.t1 MTR_3g070170 32.495 477 266 10 2 442 10 466 7.79e-72 234
MS.gene016477.t1 MTR_5g081960 32.359 479 249 9 28 447 25 487 2.41e-70 231
MS.gene016477.t1 MTR_4g015180 32.673 505 259 12 2 446 4 487 6.23e-69 227
MS.gene016477.t1 MTR_7g026850 63.871 155 53 1 35 189 12 163 9.04e-67 211
MS.gene016477.t1 MTR_4g094825 52.577 194 85 3 17 206 79 269 7.42e-60 201
MS.gene016477.t1 MTR_4g094825 51.923 156 63 2 293 447 248 392 8.52e-46 163
MS.gene016477.t1 MTR_7g026770 49.744 195 71 2 150 342 132 301 1.05e-54 184
MS.gene016477.t1 MTR_6g034485 64.615 130 41 2 195 324 1 125 4.11e-53 174
MS.gene016477.t1 MTR_6g045553 30.562 445 271 9 29 442 34 471 7.91e-52 181
MS.gene016477.t1 MTR_3g102030 33.119 311 168 7 96 400 44 320 4.26e-47 165
MS.gene016477.t1 MTR_6g034495 63.710 124 40 2 74 192 44 167 2.55e-46 158
MS.gene016477.t1 MTR_7g026830 65.476 84 29 0 92 175 15 98 3.84e-33 120
MS.gene016477.t1 MTR_4g007540 27.297 381 254 12 64 433 56 424 9.07e-32 126
MS.gene016477.t1 MTR_8g075610 26.972 393 238 13 64 434 47 412 3.50e-31 124
MS.gene016477.t1 MTR_3g026950 28.721 383 223 14 48 396 38 404 6.67e-31 124
MS.gene016477.t1 MTR_4g127650 25.792 442 292 12 18 435 19 448 2.14e-30 122
MS.gene016477.t1 MTR_2g105330 26.842 380 256 12 64 433 56 423 2.42e-30 122
MS.gene016477.t1 MTR_2g064495 26.579 380 257 12 64 433 56 423 6.97e-30 120
MS.gene016477.t1 MTR_4g097090 26.374 455 258 18 18 427 18 440 3.73e-27 113
MS.gene016477.t1 MTR_8g096530 30.321 343 209 14 64 395 59 382 1.20e-26 112
MS.gene016477.t1 MTR_3g049150 26.277 411 255 14 47 427 37 429 2.14e-24 105
MS.gene016477.t1 MTR_3g098980 25.833 360 237 10 48 396 49 389 4.40e-24 104
MS.gene016477.t1 MTR_7g026800 38.235 170 73 5 217 383 20 160 1.14e-23 98.2
MS.gene016477.t1 MTR_4g022780 37.017 181 82 5 208 385 12 163 1.93e-23 97.1
MS.gene016477.t1 MTR_7g026780 47.748 111 33 3 336 446 26 111 4.56e-23 94.0
MS.gene016477.t1 MTR_3g086020 25.658 456 283 14 4 447 5 416 6.76e-23 100
MS.gene016477.t1 MTR_7g086000 26.000 400 252 14 67 435 63 449 1.79e-21 97.1
MS.gene016477.t1 MTR_4g013050 25.496 353 205 13 62 396 51 363 4.77e-21 95.1
MS.gene016477.t1 MTR_5g076700 24.129 402 252 16 20 394 13 388 7.70e-21 95.1
MS.gene016477.t1 MTR_2g089755 26.241 423 248 17 38 408 42 452 1.40e-20 94.4
MS.gene016477.t1 MTR_1g081660 24.416 385 254 12 30 396 31 396 2.40e-20 93.6
MS.gene016477.t1 MTR_7g085930 25.610 410 253 15 56 422 51 451 2.67e-20 93.6
MS.gene016477.t1 MTR_2g089765 25.850 441 247 17 44 430 53 467 3.95e-20 93.2
MS.gene016477.t1 MTR_7g114490 24.053 449 276 18 31 444 24 442 4.85e-20 92.8
MS.gene016477.t1 MTR_7g114560 22.444 450 292 17 20 438 13 436 2.23e-19 90.5
MS.gene016477.t1 MTR_7g114550 22.616 451 290 17 20 438 13 436 2.85e-19 90.1
MS.gene016477.t1 MTR_1g040215 24.810 395 264 13 31 410 23 399 4.31e-19 89.7
MS.gene016477.t1 MTR_7g085990 26.341 410 250 17 56 422 47 447 4.42e-19 89.7
MS.gene016477.t1 MTR_7g027175 26.062 353 223 12 59 396 63 392 4.75e-19 89.4
MS.gene016477.t1 MTR_7g014330 26.464 427 249 18 27 412 23 425 7.22e-19 89.0
MS.gene016477.t1 MTR_8g012750 27.157 394 234 14 44 395 40 422 1.12e-18 88.6
MS.gene016477.t1 MTR_2g089650 26.761 426 227 18 27 398 26 420 1.43e-18 88.2
MS.gene016477.t1 MTR_5g084870 25.792 442 255 22 4 419 3 397 2.59e-18 87.4
MS.gene016477.t1 MTR_7g015860 25.616 406 239 16 64 430 64 445 4.26e-18 86.7
MS.gene016477.t1 MTR_7g114520 23.750 400 273 13 20 401 14 399 5.13e-18 86.7
MS.gene016477.t1 MTR_1g040175 23.955 359 238 12 49 394 49 385 8.31e-18 85.9
MS.gene016477.t1 MTR_3g465990 26.871 294 187 10 48 326 49 329 1.21e-17 85.5
MS.gene016477.t1 MTR_3g465980 28.328 293 184 11 48 326 49 329 2.90e-17 84.3
MS.gene016477.t1 MTR_1g040315 27.362 307 198 10 20 314 12 305 3.00e-17 84.0
MS.gene016477.t1 MTR_3g071320 22.599 354 234 10 2 336 3 335 3.21e-17 84.0
MS.gene016477.t1 MTR_1g040195 25.405 370 242 11 31 396 23 362 3.30e-17 84.0
MS.gene016477.t1 MTR_4g055170 27.103 321 207 10 48 355 49 355 3.63e-17 84.0
MS.gene016477.t1 MTR_3g112180 27.465 284 185 10 58 330 55 328 5.04e-17 83.6
MS.gene016477.t1 MTR_7g084910 28.846 260 167 8 62 312 68 318 8.53e-17 82.4
MS.gene016477.t1 MTR_8g079970 24.479 384 242 14 47 410 41 396 1.12e-16 82.4
MS.gene016477.t1 MTR_5g084850 24.946 461 289 21 4 446 3 424 1.51e-16 82.0
MS.gene016477.t1 MTR_7g114470 23.907 389 252 15 31 396 25 392 1.62e-16 82.0
MS.gene016477.t1 MTR_7g084940 25.937 347 223 13 62 395 67 392 1.83e-16 81.6
MS.gene016477.t1 MTR_7g026870 54.286 70 27 1 291 355 82 151 2.92e-16 76.3
MS.gene016477.t1 MTR_7g014230 26.545 437 255 20 27 419 25 439 8.81e-16 79.7
MS.gene016477.t1 MTR_5g026320 24.838 463 291 20 4 441 18 448 2.28e-15 78.2
MS.gene016477.t1 MTR_3g087640 25.882 425 231 19 64 433 67 462 3.74e-15 77.8
MS.gene016477.t1 MTR_1g094790 27.033 455 263 22 22 442 18 437 4.97e-15 77.4
MS.gene016477.t1 MTR_1g040320 23.604 394 226 15 18 396 18 351 6.35e-15 76.6
MS.gene016477.t1 MTR_7g056087 22.715 361 248 11 19 361 14 361 1.29e-14 75.9
MS.gene016477.t1 MTR_7g014160 24.558 452 283 17 19 427 19 455 1.72e-14 75.9
MS.gene016477.t1 MTR_2g089735 26.683 401 241 19 38 398 31 418 3.02e-14 75.1
MS.gene016477.t1 MTR_6g015315 24.522 314 205 11 147 436 140 445 3.59e-14 74.7
MS.gene016477.t1 MTR_5g090810 23.373 415 253 14 22 396 23 412 5.74e-14 73.9
MS.gene016477.t1 MTR_5g090880 23.913 368 225 13 64 396 67 414 6.20e-14 73.9
MS.gene016477.t1 MTR_7g014610 23.799 458 282 16 19 427 18 457 7.51e-14 73.6
MS.gene016477.t1 MTR_3g049140 24.413 213 142 6 224 420 12 221 1.00e-13 71.2
MS.gene016477.t1 MTR_7g026880 56.364 55 22 1 393 447 2 54 1.59e-13 65.5
MS.gene016477.t1 MTR_3g450710 24.352 386 230 13 49 396 50 411 2.52e-13 72.0
MS.gene016477.t1 MTR_5g090830 23.971 413 253 15 22 396 23 412 2.99e-13 72.0
MS.gene016477.t1 MTR_2g015430 23.324 343 235 11 67 398 61 386 5.15e-13 70.9
MS.gene016477.t1 MTR_8g079950 25.057 439 253 18 31 437 17 411 5.91e-13 70.9
MS.gene016477.t1 MTR_3g465910 27.437 277 172 10 64 326 68 329 9.78e-13 70.5
MS.gene016477.t1 MTR_7g014360 27.857 280 149 11 147 399 90 343 1.26e-12 69.7
MS.gene016477.t1 MTR_7g114960 23.888 427 278 18 47 447 44 449 4.68e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016477.t1 AT5G67150 38.976 449 253 11 4 442 5 442 2.63e-101 310
MS.gene016477.t1 AT5G67160 40.139 431 233 9 22 446 21 432 3.11e-98 301
MS.gene016477.t1 AT3G50280 38.137 451 260 9 1 443 1 440 1.69e-97 300
MS.gene016477.t1 AT3G50300 39.909 441 236 12 1 428 1 425 3.54e-95 294
MS.gene016477.t1 AT3G50270 39.578 427 245 8 28 446 27 448 1.85e-92 287
MS.gene016477.t1 AT5G42830 36.175 434 246 9 30 446 30 449 5.73e-84 265
MS.gene016477.t1 AT5G38130 37.986 437 246 11 1 427 21 442 1.95e-83 264
MS.gene016477.t1 AT2G39980 34.110 472 240 8 28 446 27 480 1.55e-79 254
MS.gene016477.t1 AT5G07860 36.427 431 246 8 30 445 31 448 1.56e-77 248
MS.gene016477.t1 AT5G01210 34.924 461 244 10 28 447 25 470 3.86e-75 243
MS.gene016477.t1 AT5G07850 36.301 438 243 13 30 446 31 453 3.52e-74 240
MS.gene016477.t1 AT3G50280 39.557 316 179 5 134 443 1 310 6.97e-70 224
MS.gene016477.t1 AT5G07870 35.281 445 246 10 30 446 31 461 1.78e-69 228
MS.gene016477.t1 AT5G23940 32.477 428 270 8 30 442 25 448 1.13e-63 213
MS.gene016477.t1 AT3G50290 42.804 271 142 6 131 393 4 269 5.20e-55 184
MS.gene016477.t1 AT2G19070 27.578 446 278 15 19 435 14 443 6.85e-33 129
MS.gene016477.t1 AT5G48930 26.683 401 261 11 49 433 41 424 1.93e-30 122
MS.gene016477.t1 AT3G48720 26.389 360 241 11 45 396 42 385 3.77e-23 101
MS.gene016477.t1 AT3G29590 25.000 448 313 11 2 437 6 442 5.28e-23 101
MS.gene016477.t1 AT1G24430 27.455 448 265 22 16 446 21 425 3.94e-22 98.6
MS.gene016477.t1 AT5G41040 26.301 346 229 13 61 396 82 411 8.86e-21 94.7
MS.gene016477.t1 AT5G41040 26.301 346 229 13 61 396 66 395 9.19e-21 94.7
MS.gene016477.t1 AT5G57840 24.194 434 292 13 18 432 12 427 1.21e-20 94.4
MS.gene016477.t1 AT5G63560 25.140 358 244 12 45 396 42 381 5.30e-20 92.4
MS.gene016477.t1 AT5G39090 27.851 456 267 21 12 433 8 435 7.62e-20 92.0
MS.gene016477.t1 AT4G15390 23.874 444 292 14 22 446 23 439 1.35e-19 91.3
MS.gene016477.t1 AT4G15390 23.874 444 292 14 22 446 23 439 1.35e-19 91.3
MS.gene016477.t1 AT3G03480 25.404 433 269 16 8 416 10 412 1.68e-19 90.9
MS.gene016477.t1 AT2G40230 25.831 391 265 13 18 398 14 389 2.10e-19 90.5
MS.gene016477.t1 AT3G29635 25.587 426 279 15 27 427 26 438 2.57e-19 90.5
MS.gene016477.t1 AT1G78990 27.446 368 224 15 49 396 52 396 3.21e-18 87.0
MS.gene016477.t1 AT1G28680 25.074 339 233 11 67 398 65 389 3.92e-18 86.7
MS.gene016477.t1 AT5G61160 24.138 435 282 16 28 433 23 438 4.31e-17 83.6
MS.gene016477.t1 AT1G28680 25.468 267 185 8 67 328 65 322 1.88e-16 81.3
MS.gene016477.t1 AT1G32910 26.503 366 209 13 62 396 63 399 2.89e-16 81.3
MS.gene016477.t1 AT1G24420 25.326 383 242 18 74 443 75 426 3.06e-16 80.9
MS.gene016477.t1 AT1G24420 25.326 383 242 18 74 443 75 426 3.06e-16 80.9
MS.gene016477.t1 AT3G26040 24.385 447 281 18 22 446 18 429 1.37e-15 79.0
MS.gene016477.t1 AT1G27620 25.818 275 187 6 64 330 58 323 3.14e-15 77.8
MS.gene016477.t1 AT5G47950 23.394 436 278 18 22 442 21 415 3.42e-15 77.8
MS.gene016477.t1 AT1G31490 25.954 393 242 19 22 395 21 383 9.58e-15 76.3
MS.gene016477.t1 AT5G16410 25.946 370 201 16 96 421 120 460 1.52e-14 75.9
MS.gene016477.t1 AT3G47170 22.695 282 192 8 64 326 70 344 5.40e-14 74.3
MS.gene016477.t1 AT1G65450 24.643 280 191 9 62 329 70 341 8.01e-13 70.5
MS.gene016477.t1 AT2G23510 24.523 367 227 17 56 398 60 400 1.03e-12 70.1

Find 89 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCCTGGTTTAATGTTTA+TGG 0.116946 8.2:-59012976 MS.gene016477:CDS
AGTACAACCCTTTGAGATTT+TGG 0.143522 8.2:+59013566 None:intergenic
GGAAATATTTGCGTGTTTAA+AGG 0.204396 8.2:-59012892 MS.gene016477:CDS
ACGTTCAAATGATGGAATTT+TGG 0.223149 8.2:+59013536 None:intergenic
TGGTAATGATCCTGAGTTTA+TGG 0.261735 8.2:-59012809 MS.gene016477:CDS
GGGAGTGAAAATAATGAAAA+TGG 0.264658 8.2:-59012913 MS.gene016477:CDS
TGGATGAAAGAGAGTGTTTA+AGG 0.274612 8.2:+59013925 None:intergenic
CCATTGAAAGAGGATTATTT+TGG 0.285934 8.2:-59013204 MS.gene016477:CDS
TCGATTGTATGATGATGATT+TGG 0.320040 8.2:+59014050 None:intergenic
GGTGATAAATAAGGCCCTTT+TGG 0.336838 8.2:+59013985 None:intergenic
AATGTTTATGGTAATGATTT+TGG 0.340175 8.2:-59012964 MS.gene016477:CDS
GTGCATTATGTGGTGGTTAT+AGG 0.349094 8.2:-59013249 MS.gene016477:CDS
AAAATTCAAAGCCAACTTAG+AGG 0.353981 8.2:-59013364 MS.gene016477:CDS
CCAAAATAATCCTCTTTCAA+TGG 0.374389 8.2:+59013204 None:intergenic
TCGAAGGGTGGTGATAAATA+AGG 0.375279 8.2:+59013976 None:intergenic
CTACTTAACGGCATCTTCAT+TGG 0.381877 8.2:-59013651 MS.gene016477:CDS
CAATTGCCTCCAAAGTTTCA+TGG 0.386784 8.2:+59012830 None:intergenic
ATCGGAAGTTGAATACCATT+TGG 0.387072 8.2:+59013507 None:intergenic
TAGCTGATGGTAAGTCGATT+TGG 0.393842 8.2:-59013608 MS.gene016477:CDS
GATCTACAATTGCTCCCATT+TGG 0.398470 8.2:-59014014 MS.gene016477:CDS
TGGACTTGGTAAAGGTGCTT+TGG 0.409489 8.2:-59013124 MS.gene016477:CDS
CATGAAACTTTGGAGGCAAT+TGG 0.418429 8.2:-59012829 MS.gene016477:CDS
AGGTGTAGAAGATGGTAGTA+TGG 0.424099 8.2:-59012872 MS.gene016477:CDS
CCCATTTGGAGTCAACCAAA+AGG 0.428400 8.2:-59014000 MS.gene016477:CDS
GGAAACCAACGTTCAAATGA+TGG 0.431017 8.2:+59013528 None:intergenic
ATACTAGAGGCAACATTACT+TGG 0.433898 8.2:+59012777 None:intergenic
TTGTAGTGGTGGAGAGGACT+TGG 0.449037 8.2:+59014102 None:intergenic
TTTGGCATTTCATCAATTCA+TGG 0.451540 8.2:-59013590 MS.gene016477:CDS
CGATTGTATGATGATGATTT+GGG 0.466946 8.2:+59014051 None:intergenic
TTTGGTTGGGGAAAACCTGT+GGG 0.472290 8.2:-59012946 MS.gene016477:CDS
AATGAAACATCCTCTCATAT+AGG 0.477302 8.2:+59013412 None:intergenic
AAAGCCATTACTTGCAGTCC+AGG 0.484404 8.2:-59013682 MS.gene016477:CDS
GTTGATTCCTCCATTGAAAG+AGG 0.486725 8.2:-59013214 MS.gene016477:CDS
CTTTGTACCAGCCTCTAAGT+TGG 0.486862 8.2:+59013353 None:intergenic
TTTGAACGTTGGTTTCCAAA+TGG 0.487730 8.2:-59013522 MS.gene016477:CDS
TAGGATGATAAGTAGCTCCT+AGG 0.499348 8.2:+59013760 None:intergenic
CATAATGCACTTCTTCTTGA+GGG 0.502724 8.2:+59013262 None:intergenic
CAAGACAATGGACTTGGTAA+AGG 0.507284 8.2:-59013132 MS.gene016477:CDS
TGCAGGTCGTCTCAAAATCA+AGG 0.510077 8.2:-59013880 MS.gene016477:CDS
TTGGCATTTCATCAATTCAT+GGG 0.510084 8.2:-59013589 MS.gene016477:CDS
CAGGTAACAGAACTACTTAA+CGG 0.511669 8.2:-59013663 MS.gene016477:CDS
TTCAAAGCCAACTTAGAGGC+TGG 0.516313 8.2:-59013360 MS.gene016477:CDS
GTGTTTAAAGGTGTAGAAGA+TGG 0.522048 8.2:-59012880 MS.gene016477:CDS
CGTTAAGTAGTTCTGTTACC+TGG 0.524596 8.2:+59013664 None:intergenic
GTTACCTGGACTGCAAGTAA+TGG 0.527795 8.2:+59013678 None:intergenic
GCACTTCTTCTTGAGGGTCA+AGG 0.528530 8.2:+59013268 None:intergenic
TTCAATGGAGGAATCAACCT+TGG 0.531124 8.2:+59013219 None:intergenic
ACTAGTGTCGATGATATCCT+AGG 0.532387 8.2:-59013777 MS.gene016477:CDS
AATTGCCTCCAAAGTTTCAT+GGG 0.532415 8.2:+59012831 None:intergenic
TTGTGCGTGGATTGTAGTGG+TGG 0.533208 8.2:+59014091 None:intergenic
GTGTTTGTCCCATGAAACTT+TGG 0.534872 8.2:-59012839 MS.gene016477:CDS
GTACAATAGTAAAACATTAG+TGG 0.537819 8.2:-59013007 MS.gene016477:CDS
TACAATAGTAAAACATTAGT+GGG 0.542055 8.2:-59013006 MS.gene016477:CDS
TCCGTTGTTAGCATGAAAGT+TGG 0.545219 8.2:-59013165 MS.gene016477:CDS
AAATTCCATCATTTGAACGT+TGG 0.545341 8.2:-59013533 MS.gene016477:CDS
TCCATCACATGCAACAATGC+CGG 0.551602 8.2:-59013831 MS.gene016477:CDS
CCATTTGGAGTCAACCAAAA+GGG 0.559855 8.2:-59013999 MS.gene016477:CDS
ACATAATGCACTTCTTCTTG+AGG 0.564314 8.2:+59013261 None:intergenic
TTGGTTGGGGAAAACCTGTG+GGG 0.567130 8.2:-59012945 MS.gene016477:CDS
TCATGGGCCAAAATCTCAAA+GGG 0.568456 8.2:-59013573 MS.gene016477:CDS
TTTGATGTGTCTAGTTTCGA+AGG 0.568899 8.2:+59013960 None:intergenic
TTCATGGGCCAAAATCTCAA+AGG 0.573275 8.2:-59013574 MS.gene016477:CDS
ATAGTGAAAGGAAATCGAAT+CGG 0.575488 8.2:+59013489 None:intergenic
TGGTTGGGGAAAACCTGTGG+GGG 0.578502 8.2:-59012944 MS.gene016477:CDS
ATTAATCATGCAGTAGCTGA+TGG 0.579572 8.2:-59013621 MS.gene016477:CDS
ACCGGCATTGTTGCATGTGA+TGG 0.582691 8.2:+59013830 None:intergenic
TTTGTCCCATGAAACTTTGG+AGG 0.591981 8.2:-59012836 MS.gene016477:CDS
AAATAATCCTCTTTCAATGG+AGG 0.592205 8.2:+59013207 None:intergenic
ATGTGTCTAGTTTCGAAGGG+TGG 0.597172 8.2:+59013964 None:intergenic
TACAATCGAGCTAACACCAT+GGG 0.600648 8.2:-59014036 MS.gene016477:CDS
TTGTTGCAAGACAATGGACT+TGG 0.601437 8.2:-59013138 MS.gene016477:CDS
GGGAGCAATTGTAGATCCCA+TGG 0.605618 8.2:+59014020 None:intergenic
ATACCAAGAGTAATAAAACT+AGG 0.607573 8.2:+59013030 None:intergenic
GGTGATTTGTTGCAAGACAA+TGG 0.609643 8.2:-59013144 MS.gene016477:CDS
AGCATGAACAAATAGTGCAC+CGG 0.613200 8.2:+59013812 None:intergenic
TGGTTATAGGCGTTAGACCA+AGG 0.625694 8.2:-59013236 MS.gene016477:CDS
TTGATGTGTCTAGTTTCGAA+GGG 0.626200 8.2:+59013961 None:intergenic
TTGAGACGACCTGCAAAGGG+CGG 0.627335 8.2:+59013888 None:intergenic
TCAAGAAGAAGTGCATTATG+TGG 0.629418 8.2:-59013259 MS.gene016477:CDS
GTCACAACATCCATAAACTC+AGG 0.634083 8.2:+59012799 None:intergenic
AGAAGAAGTGCATTATGTGG+TGG 0.641210 8.2:-59013256 MS.gene016477:CDS
TGATGATGATTTGGGTCACA+AGG 0.653197 8.2:+59014059 None:intergenic
CGTGGATTGTAGTGGTGGAG+AGG 0.660519 8.2:+59014096 None:intergenic
TAGTGGGTAGTAGTTCACCC+TGG 0.662355 8.2:-59012990 MS.gene016477:CDS
ATTACCATAAACATTAAACC+AGG 0.672703 8.2:+59012972 None:intergenic
ACCAACTTTCATGCTAACAA+CGG 0.674514 8.2:+59013164 None:intergenic
AGTGGTGGAGAGGACTTGGA+TGG 0.683619 8.2:+59014106 None:intergenic
TTACCATAAACATTAAACCA+GGG 0.701842 8.2:+59012973 None:intergenic
ATACAATCGAGCTAACACCA+TGG 0.743736 8.2:-59014037 MS.gene016477:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTACAATTATTATTTTG+TGG + chr8.2:59013446-59013465 None:intergenic 10.0%
!! TACAATAGTAAAACATTAGT+GGG - chr8.2:59013899-59013918 MS.gene016477:CDS 20.0%
!!! AAAAATGAGTGAAGAATTTT+AGG + chr8.2:59013167-59013186 None:intergenic 20.0%
!!! AATGTTTATGGTAATGATTT+TGG - chr8.2:59013941-59013960 MS.gene016477:CDS 20.0%
!!! CACTCATTTTTTCAATTTAA+TGG - chr8.2:59013176-59013195 MS.gene016477:CDS 20.0%
! AATGGAATGAAAAATTACGA+AGG - chr8.2:59013194-59013213 MS.gene016477:CDS 25.0%
! ATACCAAGAGTAATAAAACT+AGG + chr8.2:59013878-59013897 None:intergenic 25.0%
! ATGGAATGAAAAATTACGAA+GGG - chr8.2:59013195-59013214 MS.gene016477:CDS 25.0%
! ATTACCATAAACATTAAACC+AGG + chr8.2:59013936-59013955 None:intergenic 25.0%
! GTACAATAGTAAAACATTAG+TGG - chr8.2:59013898-59013917 MS.gene016477:CDS 25.0%
! TTACCATAAACATTAAACCA+GGG + chr8.2:59013935-59013954 None:intergenic 25.0%
!!! TTATGGTAATGATTTTGGTT+GGG - chr8.2:59013946-59013965 MS.gene016477:CDS 25.0%
!!! TTTATGGTAATGATTTTGGT+TGG - chr8.2:59013945-59013964 MS.gene016477:CDS 25.0%
AAAAACTCAACCTATATGAG+AGG - chr8.2:59013483-59013502 MS.gene016477:CDS 30.0%
AAAATTCAAAGCCAACTTAG+AGG - chr8.2:59013541-59013560 MS.gene016477:CDS 30.0%
AAATAATCCTCTTTCAATGG+AGG + chr8.2:59013701-59013720 None:intergenic 30.0%
AAATTCCATCATTTGAACGT+TGG - chr8.2:59013372-59013391 MS.gene016477:CDS 30.0%
AATGAAACATCCTCTCATAT+AGG + chr8.2:59013496-59013515 None:intergenic 30.0%
ATAGTGAAAGGAAATCGAAT+CGG + chr8.2:59013419-59013438 None:intergenic 30.0%
CCAAAATAATCCTCTTTCAA+TGG + chr8.2:59013704-59013723 None:intergenic 30.0%
GGAAATATTTGCGTGTTTAA+AGG - chr8.2:59014013-59014032 MS.gene016477:CDS 30.0%
GGGAGTGAAAATAATGAAAA+TGG - chr8.2:59013992-59014011 MS.gene016477:CDS 30.0%
TGAAAAATGACTATGAGAGT+TGG - chr8.2:59013843-59013862 MS.gene016477:CDS 30.0%
TTGGCATTTCATCAATTCAT+GGG - chr8.2:59013316-59013335 MS.gene016477:CDS 30.0%
TTTGGCATTTCATCAATTCA+TGG - chr8.2:59013315-59013334 MS.gene016477:CDS 30.0%
! AAGCACTTTTCACACATATT+TGG - chr8.2:59013591-59013610 MS.gene016477:CDS 30.0%
! CCATTGAAAGAGGATTATTT+TGG - chr8.2:59013701-59013720 MS.gene016477:CDS 30.0%
! CGATTGTATGATGATGATTT+GGG + chr8.2:59012857-59012876 None:intergenic 30.0%
! TCGATTGTATGATGATGATT+TGG + chr8.2:59012858-59012877 None:intergenic 30.0%
! TTTTGTGGTTGTATAGTGAA+AGG + chr8.2:59013431-59013450 None:intergenic 30.0%
!! ACGTTCAAATGATGGAATTT+TGG + chr8.2:59013372-59013391 None:intergenic 30.0%
!!! ACACCTAGTTTTATTACTCT+TGG - chr8.2:59013872-59013891 MS.gene016477:CDS 30.0%
!!! TATGGTAATGATTTTGGTTG+GGG - chr8.2:59013947-59013966 MS.gene016477:CDS 30.0%
AAAGGGCGGAAAAAATTCAA+GGG + chr8.2:59013006-59013025 None:intergenic 35.0%
AATTGCCTCCAAAGTTTCAT+GGG + chr8.2:59014077-59014096 None:intergenic 35.0%
ACATAATGCACTTCTTCTTG+AGG + chr8.2:59013647-59013666 None:intergenic 35.0%
ACCAACTTTCATGCTAACAA+CGG + chr8.2:59013744-59013763 None:intergenic 35.0%
ATCGGAAGTTGAATACCATT+TGG + chr8.2:59013401-59013420 None:intergenic 35.0%
ATTAATCATGCAGTAGCTGA+TGG - chr8.2:59013284-59013303 MS.gene016477:CDS 35.0%
CAGGTAACAGAACTACTTAA+CGG - chr8.2:59013242-59013261 MS.gene016477:CDS 35.0%
CATAATGCACTTCTTCTTGA+GGG + chr8.2:59013646-59013665 None:intergenic 35.0%
TCACCCTGGTTTAATGTTTA+TGG - chr8.2:59013929-59013948 MS.gene016477:CDS 35.0%
! GTGTTTAAAGGTGTAGAAGA+TGG - chr8.2:59014025-59014044 MS.gene016477:CDS 35.0%
! TCAAGAAGAAGTGCATTATG+TGG - chr8.2:59013646-59013665 MS.gene016477:CDS 35.0%
! TGGTAATGATCCTGAGTTTA+TGG - chr8.2:59014096-59014115 MS.gene016477:CDS 35.0%
! TTGATGTGTCTAGTTTCGAA+GGG + chr8.2:59012947-59012966 None:intergenic 35.0%
! TTTGATGTGTCTAGTTTCGA+AGG + chr8.2:59012948-59012967 None:intergenic 35.0%
!! TGGATGAAAGAGAGTGTTTA+AGG + chr8.2:59012983-59013002 None:intergenic 35.0%
!! TTTGAACGTTGGTTTCCAAA+TGG - chr8.2:59013383-59013402 MS.gene016477:CDS 35.0%
!!! AGTACAACCCTTTGAGATTT+TGG + chr8.2:59013342-59013361 None:intergenic 35.0%
AGCATGAACAAATAGTGCAC+CGG + chr8.2:59013096-59013115 None:intergenic 40.0%
AGGTGTAGAAGATGGTAGTA+TGG - chr8.2:59014033-59014052 MS.gene016477:CDS 40.0%
ATACAATCGAGCTAACACCA+TGG - chr8.2:59012868-59012887 MS.gene016477:CDS 40.0%
CAAAGGGCGGAAAAAATTCA+AGG + chr8.2:59013007-59013026 None:intergenic 40.0%
CAATTGCCTCCAAAGTTTCA+TGG + chr8.2:59014078-59014097 None:intergenic 40.0%
CTACTTAACGGCATCTTCAT+TGG - chr8.2:59013254-59013273 MS.gene016477:CDS 40.0%
GATCTACAATTGCTCCCATT+TGG - chr8.2:59012891-59012910 MS.gene016477:CDS 40.0%
GGAAACCAACGTTCAAATGA+TGG + chr8.2:59013380-59013399 None:intergenic 40.0%
GTCACAACATCCATAAACTC+AGG + chr8.2:59014109-59014128 None:intergenic 40.0%
GTGCATTATGTGGTGGTTAT+AGG - chr8.2:59013656-59013675 MS.gene016477:CDS 40.0%
GTGTTTGTCCCATGAAACTT+TGG - chr8.2:59014066-59014085 MS.gene016477:CDS 40.0%
TACAATCGAGCTAACACCAT+GGG - chr8.2:59012869-59012888 MS.gene016477:CDS 40.0%
TAGCTGATGGTAAGTCGATT+TGG - chr8.2:59013297-59013316 MS.gene016477:CDS 40.0%
TAGGATGATAAGTAGCTCCT+AGG + chr8.2:59013148-59013167 None:intergenic 40.0%
TCATGGGCCAAAATCTCAAA+GGG - chr8.2:59013332-59013351 MS.gene016477:CDS 40.0%
TCCGTTGTTAGCATGAAAGT+TGG - chr8.2:59013740-59013759 MS.gene016477:CDS 40.0%
TCGAAGGGTGGTGATAAATA+AGG + chr8.2:59012932-59012951 None:intergenic 40.0%
TTCAATGGAGGAATCAACCT+TGG + chr8.2:59013689-59013708 None:intergenic 40.0%
TTCATGGGCCAAAATCTCAA+AGG - chr8.2:59013331-59013350 MS.gene016477:CDS 40.0%
TTTGTCCCATGAAACTTTGG+AGG - chr8.2:59014069-59014088 MS.gene016477:CDS 40.0%
! AAGGATTGTGATTTTGTGCG+TGG + chr8.2:59012830-59012849 None:intergenic 40.0%
! AGAAGAAGTGCATTATGTGG+TGG - chr8.2:59013649-59013668 MS.gene016477:CDS 40.0%
! CAAGACAATGGACTTGGTAA+AGG - chr8.2:59013773-59013792 MS.gene016477:CDS 40.0%
! CATGAAACTTTGGAGGCAAT+TGG - chr8.2:59014076-59014095 MS.gene016477:CDS 40.0%
! CCATTTGGAGTCAACCAAAA+GGG - chr8.2:59012906-59012925 MS.gene016477:CDS 40.0%
! CGTTAAGTAGTTCTGTTACC+TGG + chr8.2:59013244-59013263 None:intergenic 40.0%
! GGTGATAAATAAGGCCCTTT+TGG + chr8.2:59012923-59012942 None:intergenic 40.0%
! GTTGATTCCTCCATTGAAAG+AGG - chr8.2:59013691-59013710 MS.gene016477:CDS 40.0%
! TGATGATGATTTGGGTCACA+AGG + chr8.2:59012849-59012868 None:intergenic 40.0%
! TTCTTGAGGGTCAAGGTTTT+TGG + chr8.2:59013633-59013652 None:intergenic 40.0%
! TTGTTGCAAGACAATGGACT+TGG - chr8.2:59013767-59013786 MS.gene016477:CDS 40.0%
!! ACTAGTGTCGATGATATCCT+AGG - chr8.2:59013128-59013147 MS.gene016477:CDS 40.0%
!! ATTTTGTGCGTGGATTGTAG+TGG + chr8.2:59012820-59012839 None:intergenic 40.0%
!! CCTTTTGGTTGACTCCAAAT+GGG + chr8.2:59012908-59012927 None:intergenic 40.0%
!! GGTGATTTGTTGCAAGACAA+TGG - chr8.2:59013761-59013780 MS.gene016477:CDS 40.0%
!!! ATTTTGAGACGACCTGCAAA+GGG + chr8.2:59013023-59013042 None:intergenic 40.0%
AAAGCCATTACTTGCAGTCC+AGG - chr8.2:59013223-59013242 MS.gene016477:CDS 45.0%
CTTTGTACCAGCCTCTAAGT+TGG + chr8.2:59013555-59013574 None:intergenic 45.0%
GTTACCTGGACTGCAAGTAA+TGG + chr8.2:59013230-59013249 None:intergenic 45.0%
TCCATCACATGCAACAATGC+CGG - chr8.2:59013074-59013093 MS.gene016477:CDS 45.0%
TGCAGGTCGTCTCAAAATCA+AGG - chr8.2:59013025-59013044 MS.gene016477:CDS 45.0%
TGGTTATAGGCGTTAGACCA+AGG - chr8.2:59013669-59013688 MS.gene016477:CDS 45.0%
TTCAAAGCCAACTTAGAGGC+TGG - chr8.2:59013545-59013564 MS.gene016477:CDS 45.0%
TTTGGTTGGGGAAAACCTGT+GGG - chr8.2:59013959-59013978 MS.gene016477:CDS 45.0%
! ATGTGTCTAGTTTCGAAGGG+TGG + chr8.2:59012944-59012963 None:intergenic 45.0%
! CCCATTTGGAGTCAACCAAA+AGG - chr8.2:59012905-59012924 MS.gene016477:CDS 45.0%
!! CCCTTTTGGTTGACTCCAAA+TGG + chr8.2:59012909-59012928 None:intergenic 45.0%
!! TGGACTTGGTAAAGGTGCTT+TGG - chr8.2:59013781-59013800 MS.gene016477:CDS 45.0%
!! TTTTGGTTGGGGAAAACCTG+TGG - chr8.2:59013958-59013977 MS.gene016477:CDS 45.0%
!!! GAATTTTTTCCGCCCTTTGC+AGG - chr8.2:59013008-59013027 MS.gene016477:CDS 45.0%
!!! GATTTTGAGACGACCTGCAA+AGG + chr8.2:59013024-59013043 None:intergenic 45.0%
ACCGGCATTGTTGCATGTGA+TGG + chr8.2:59013078-59013097 None:intergenic 50.0%
GCACTTCTTCTTGAGGGTCA+AGG + chr8.2:59013640-59013659 None:intergenic 50.0%
GGGAGCAATTGTAGATCCCA+TGG + chr8.2:59012888-59012907 None:intergenic 50.0%
GGGCGGAAAAAATTCAAGGG+TGG + chr8.2:59013003-59013022 None:intergenic 50.0%
TTGGTTGGGGAAAACCTGTG+GGG - chr8.2:59013960-59013979 MS.gene016477:CDS 50.0%
! TAGTGGGTAGTAGTTCACCC+TGG - chr8.2:59013915-59013934 MS.gene016477:CDS 50.0%
! TTGTAGTGGTGGAGAGGACT+TGG + chr8.2:59012806-59012825 None:intergenic 50.0%
! TTGTGCGTGGATTGTAGTGG+TGG + chr8.2:59012817-59012836 None:intergenic 50.0%
AAACCTGTGGGGGTTCGAAC+TGG - chr8.2:59013971-59013990 MS.gene016477:CDS 55.0%
AACCTGTGGGGGTTCGAACT+GGG - chr8.2:59013972-59013991 MS.gene016477:CDS 55.0%
TGGTTGGGGAAAACCTGTGG+GGG - chr8.2:59013961-59013980 MS.gene016477:CDS 55.0%
TTGAGACGACCTGCAAAGGG+CGG + chr8.2:59013020-59013039 None:intergenic 55.0%
! AGTGGTGGAGAGGACTTGGA+TGG + chr8.2:59012802-59012821 None:intergenic 55.0%
! CGTGGATTGTAGTGGTGGAG+AGG + chr8.2:59012812-59012831 None:intergenic 55.0%
CTCCCAGTTCGAACCCCCAC+AGG + chr8.2:59013977-59013996 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 59012792 59014135 59012792 ID=MS.gene016477
chr8.2 mRNA 59012792 59014135 59012792 ID=MS.gene016477.t1;Parent=MS.gene016477
chr8.2 exon 59012792 59014135 59012792 ID=MS.gene016477.t1.exon1;Parent=MS.gene016477.t1
chr8.2 CDS 59012792 59014135 59012792 ID=cds.MS.gene016477.t1;Parent=MS.gene016477.t1
Gene Sequence

>MS.gene016477

ATGAGTTCCATCCAAGTCCTCTCCACCACTACAATCCACGCACAAAATCACAATCCTTGTGACCCAAATCATCATCATACAATCGAGCTAACACCATGGGATCTACAATTGCTCCCATTTGGAGTCAACCAAAAGGGCCTTATTTATCACCACCCTTCGAAACTAGACACATCAAATCAAATTGAACACCTTAAACACTCTCTTTCATCCACCCTTGAATTTTTTCCGCCCTTTGCAGGTCGTCTCAAAATCAAGGAACACGAAGATAACATTATCTCATGTTCCATCACATGCAACAATGCCGGTGCACTATTTGTTCATGCTGCAGCAGAAAATACTAGTGTCGATGATATCCTAGGAGCTACTTATCATCCTAAAATTCTTCACTCATTTTTTCAATTTAATGGAATGAAAAATTACGAAGGGACATCAAAGCCATTACTTGCAGTCCAGGTAACAGAACTACTTAACGGCATCTTCATTGGTTGCACAATTAATCATGCAGTAGCTGATGGTAAGTCGATTTGGCATTTCATCAATTCATGGGCCAAAATCTCAAAGGGTTGTACTGAAATATCCAAAATTCCATCATTTGAACGTTGGTTTCCAAATGGTATTCAACTTCCGATTCGATTTCCTTTCACTATACAACCACAAAATAATAATTGTAATAATGATAATCATAAAGAAGAAAAACTCAACCTATATGAGAGGATGTTTCATTTCACAAAAGAGAATATTGCAAAACTAAAATTCAAAGCCAACTTAGAGGCTGGTACAAAGAACATATCTTCTTTGCAAGCACTTTTCACACATATTTGGCGATCTATTATACGTTCCAAAAACCTTGACCCTCAAGAAGAAGTGCATTATGTGGTGGTTATAGGCGTTAGACCAAGGTTGATTCCTCCATTGAAAGAGGATTATTTTGGTAATGCTATGATAGACTCCGTTGTTAGCATGAAAGTTGGTGATTTGTTGCAAGACAATGGACTTGGTAAAGGTGCTTTGGAGATGAACAAGATGATTGCTTTGCAATCTGATGAGATGTTGAAAAATGACTATGAGAGTTGGTTGAAAACACCTAGTTTTATTACTCTTGGTATGTACAATAGTAAAACATTAGTGGGTAGTAGTTCACCCTGGTTTAATGTTTATGGTAATGATTTTGGTTGGGGAAAACCTGTGGGGGTTCGAACTGGGAGTGAAAATAATGAAAATGGAAATATTTGCGTGTTTAAAGGTGTAGAAGATGGTAGTATGGATCTTGAAGTGTGTTTGTCCCATGAAACTTTGGAGGCAATTGGTAATGATCCTGAGTTTATGGATGTTGTGACCAAGTAA
Protein sequence

>MS.gene016477.t1

MSSIQVLSTTTIHAQNHNPCDPNHHHTIELTPWDLQLLPFGVNQKGLIYHHPSKLDTSNQIEHLKHSLSSTLEFFPPFAGRLKIKEHEDNIISCSITCNNAGALFVHAAAENTSVDDILGATYHPKILHSFFQFNGMKNYEGTSKPLLAVQVTELLNGIFIGCTINHAVADGKSIWHFINSWAKISKGCTEISKIPSFERWFPNGIQLPIRFPFTIQPQNNNCNNDNHKEEKLNLYERMFHFTKENIAKLKFKANLEAGTKNISSLQALFTHIWRSIIRSKNLDPQEEVHYVVVIGVRPRLIPPLKEDYFGNAMIDSVVSMKVGDLLQDNGLGKGALEMNKMIALQSDEMLKNDYESWLKTPSFITLGMYNSKTLVGSSSPWFNVYGNDFGWGKPVGVRTGSENNENGNICVFKGVEDGSMDLEVCLSHETLEAIGNDPEFMDVVTK