AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87532

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87532.t1 MTR_4g022670 94.344 442 25 0 1 442 1 442 0.0 863
MS.gene87532.t1 MTR_4g022800 83.070 443 74 1 1 442 1 443 0.0 773
MS.gene87532.t1 MTR_4g022720 80.995 442 78 1 1 442 1 436 0.0 746
MS.gene87532.t1 MTR_7g026860 78.054 442 94 1 1 442 1 439 0.0 728
MS.gene87532.t1 MTR_4g022760 76.018 442 62 1 1 442 1 398 0.0 686
MS.gene87532.t1 MTR_4g022825 74.091 440 113 1 1 440 1 439 0.0 675
MS.gene87532.t1 MTR_3g088420 66.968 442 138 2 1 442 1 434 0.0 605
MS.gene87532.t1 MTR_3g026310 65.033 449 150 2 1 442 1 449 0.0 593
MS.gene87532.t1 MTR_8g041770 58.940 453 164 6 1 442 1 442 0.0 523
MS.gene87532.t1 MTR_0190s0020 57.743 452 170 7 1 441 1 442 0.0 520
MS.gene87532.t1 MTR_4g094842 56.674 457 169 9 1 439 1 446 5.44e-169 482
MS.gene87532.t1 MTR_4g094835 53.187 455 182 8 1 437 1 442 5.69e-161 462
MS.gene87532.t1 MTR_4g022290 51.410 461 184 12 1 439 1 443 3.73e-149 431
MS.gene87532.t1 MTR_6g016815 52.715 442 135 7 1 442 15 382 3.56e-148 427
MS.gene87532.t1 MTR_4g094838 49.672 457 178 9 4 437 1 428 2.95e-145 421
MS.gene87532.t1 MTR_4g094828 51.528 458 197 12 1 441 1 450 3.28e-144 419
MS.gene87532.t1 MTR_8g079710 51.333 450 187 10 1 432 1 436 6.14e-144 418
MS.gene87532.t1 MTR_4g094832 56.579 380 138 7 72 437 60 426 3.95e-141 410
MS.gene87532.t1 MTR_3g101900 39.726 438 237 11 18 441 24 448 2.48e-93 289
MS.gene87532.t1 MTR_3g101930 38.341 446 241 10 16 440 22 454 8.52e-90 280
MS.gene87532.t1 MTR_8g040760 64.319 213 69 3 1 207 1 212 2.16e-88 268
MS.gene87532.t1 MTR_7g026850 73.410 173 45 1 23 195 7 178 2.20e-87 264
MS.gene87532.t1 MTR_3g102020 38.462 442 245 9 16 441 21 451 1.23e-86 272
MS.gene87532.t1 MTR_7g026770 67.172 198 37 1 141 338 132 301 2.15e-85 263
MS.gene87532.t1 MTR_3g101960 38.073 436 244 9 16 436 21 445 7.75e-84 265
MS.gene87532.t1 MTR_3g101970 37.358 439 238 9 16 440 21 436 3.83e-83 262
MS.gene87532.t1 MTR_3g102010 36.961 441 252 9 16 441 22 451 2.38e-82 261
MS.gene87532.t1 MTR_8g040780 57.407 216 88 2 225 436 12 227 2.39e-78 244
MS.gene87532.t1 MTR_3g070170 31.545 466 251 13 16 437 25 466 3.55e-58 198
MS.gene87532.t1 MTR_4g015180 31.493 489 254 13 16 442 19 488 4.72e-57 196
MS.gene87532.t1 MTR_5g081960 30.061 489 258 13 16 441 19 486 6.60e-56 192
MS.gene87532.t1 MTR_4g094825 51.852 189 82 5 17 199 84 269 7.98e-53 182
MS.gene87532.t1 MTR_4g094825 47.953 171 66 3 269 437 238 387 2.10e-44 159
MS.gene87532.t1 MTR_6g034485 61.364 132 43 1 189 320 2 125 5.48e-49 163
MS.gene87532.t1 MTR_6g045553 28.541 466 293 11 4 437 14 471 5.67e-48 171
MS.gene87532.t1 MTR_7g026800 54.286 175 48 5 208 380 16 160 2.11e-47 161
MS.gene87532.t1 MTR_3g102030 35.161 310 162 5 90 397 48 320 5.40e-47 164
MS.gene87532.t1 MTR_4g022780 53.801 171 48 4 208 377 17 157 9.98e-47 159
MS.gene87532.t1 MTR_6g034495 59.124 137 46 3 54 180 28 164 2.25e-45 155
MS.gene87532.t1 MTR_7g026780 62.162 111 18 1 332 442 26 112 1.61e-39 138
MS.gene87532.t1 MTR_7g026830 78.571 84 18 0 83 166 15 98 8.12e-39 135
MS.gene87532.t1 MTR_7g026870 100.000 55 0 0 297 351 97 151 3.45e-32 120
MS.gene87532.t1 MTR_8g075610 26.385 379 260 11 55 428 47 411 3.82e-30 121
MS.gene87532.t1 MTR_4g007540 26.531 392 247 13 55 428 56 424 4.43e-29 118
MS.gene87532.t1 MTR_2g105330 26.053 380 263 10 55 428 56 423 1.05e-28 117
MS.gene87532.t1 MTR_2g064495 26.053 380 263 10 55 428 56 423 2.48e-28 116
MS.gene87532.t1 MTR_3g098980 29.296 355 204 13 55 393 66 389 1.91e-27 114
MS.gene87532.t1 MTR_8g096530 28.029 421 231 17 53 436 57 442 2.71e-27 113
MS.gene87532.t1 MTR_7g026880 90.385 52 5 0 390 441 2 53 1.07e-26 102
MS.gene87532.t1 MTR_3g026950 26.371 383 212 16 52 393 51 404 7.70e-23 100
MS.gene87532.t1 MTR_4g013050 27.424 361 200 13 50 393 48 363 7.70e-23 100
MS.gene87532.t1 MTR_7g027175 25.974 385 251 11 55 428 68 429 4.03e-20 92.4
MS.gene87532.t1 MTR_3g049150 25.823 395 260 13 52 426 52 433 1.82e-19 90.9
MS.gene87532.t1 MTR_1g081660 25.168 298 198 8 24 308 31 316 7.18e-19 89.0
MS.gene87532.t1 MTR_7g114470 22.864 433 282 15 25 428 25 434 5.20e-17 83.2
MS.gene87532.t1 MTR_7g014330 23.963 434 267 15 1 396 5 413 7.83e-17 82.8
MS.gene87532.t1 MTR_3g087640 24.494 445 291 12 24 432 31 466 2.23e-16 81.6
MS.gene87532.t1 MTR_2g089755 24.554 448 284 18 24 432 37 469 4.38e-16 80.5
MS.gene87532.t1 MTR_1g040195 25.178 421 260 20 25 428 23 405 4.41e-16 80.5
MS.gene87532.t1 MTR_2g089670 25.130 386 242 13 38 392 60 429 4.93e-16 80.5
MS.gene87532.t1 MTR_2g089765 24.272 412 253 15 24 392 39 434 9.91e-16 79.7
MS.gene87532.t1 MTR_6g015830 24.306 432 287 13 22 432 42 454 1.06e-15 79.3
MS.gene87532.t1 MTR_7g014250 24.138 406 251 13 22 396 34 413 1.50e-15 79.0
MS.gene87532.t1 MTR_2g089650 24.664 446 278 18 8 418 21 443 1.53e-15 79.0
MS.gene87532.t1 MTR_1g040175 27.536 276 178 11 55 324 64 323 2.06e-15 78.6
MS.gene87532.t1 MTR_4g097090 23.148 432 269 14 8 398 4 413 3.37e-15 77.8
MS.gene87532.t1 MTR_7g114520 22.622 389 260 14 25 393 25 392 4.69e-15 77.4
MS.gene87532.t1 MTR_7g085930 23.117 385 259 9 43 399 47 422 5.61e-15 77.0
MS.gene87532.t1 MTR_7g084910 27.044 318 181 14 53 341 68 363 6.43e-15 76.6
MS.gene87532.t1 MTR_7g015860 23.908 435 260 17 4 393 5 413 7.42e-15 76.6
MS.gene87532.t1 MTR_6g015320 26.316 304 186 11 138 414 143 435 8.12e-15 76.6
MS.gene87532.t1 MTR_4g127650 23.544 395 255 15 55 422 66 440 1.01e-14 76.3
MS.gene87532.t1 MTR_7g084940 25.952 289 194 10 53 335 67 341 1.15e-14 76.3
MS.gene87532.t1 MTR_1g040315 26.578 301 186 13 25 309 23 304 1.68e-14 75.5
MS.gene87532.t1 MTR_7g085990 23.650 389 252 13 43 399 43 418 3.61e-14 74.7
MS.gene87532.t1 MTR_7g086000 25.067 375 235 16 58 406 63 417 5.38e-14 73.9
MS.gene87532.t1 MTR_1g040320 24.931 361 200 14 44 393 51 351 7.55e-14 73.6
MS.gene87532.t1 MTR_3g465990 25.000 304 195 11 55 344 68 352 7.88e-14 73.6
MS.gene87532.t1 MTR_3g465930 26.190 294 190 11 55 339 69 344 4.63e-13 71.2
MS.gene87532.t1 MTR_3g465910 25.965 285 186 10 55 330 68 336 6.97e-13 70.9
MS.gene87532.t1 MTR_5g090810 23.377 385 240 13 43 393 49 412 1.00e-12 70.1
MS.gene87532.t1 MTR_5g090830 23.896 385 238 13 43 393 49 412 1.03e-12 70.1
MS.gene87532.t1 MTR_6g053810 25.267 281 184 10 55 324 71 336 1.53e-12 69.7
MS.gene87532.t1 MTR_3g112180 22.897 428 296 15 11 428 23 426 3.60e-12 68.6
MS.gene87532.t1 MTR_7g014200 24.186 430 254 17 15 396 19 424 2.60e-11 65.9
MS.gene87532.t1 MTR_7g014610 24.169 331 195 13 149 442 158 469 4.64e-11 65.1
MS.gene87532.t1 MTR_5g090880 21.995 441 280 16 43 441 49 467 6.49e-11 64.7
MS.gene87532.t1 MTR_5g084850 24.292 424 244 19 4 397 3 379 6.84e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87532.t1 AT5G67150 39.468 451 247 12 2 437 3 442 1.02e-95 295
MS.gene87532.t1 AT3G50300 41.324 438 227 14 1 422 1 424 3.23e-92 286
MS.gene87532.t1 AT5G67160 38.636 440 247 11 6 441 12 432 1.73e-88 276
MS.gene87532.t1 AT3G50280 38.551 428 240 10 22 438 25 440 1.75e-87 274
MS.gene87532.t1 AT5G42830 37.955 440 249 11 15 441 21 449 5.81e-86 270
MS.gene87532.t1 AT3G50270 38.359 451 254 11 6 441 7 448 6.85e-85 267
MS.gene87532.t1 AT5G07850 39.101 445 242 12 15 442 22 454 1.99e-81 258
MS.gene87532.t1 AT5G07860 37.162 444 244 9 15 440 22 448 1.09e-79 254
MS.gene87532.t1 AT5G07870 37.339 466 254 13 4 442 8 462 9.71e-76 244
MS.gene87532.t1 AT5G38130 36.721 433 246 12 4 422 24 442 1.22e-75 244
MS.gene87532.t1 AT2G39980 32.839 472 261 14 16 442 21 481 3.19e-66 219
MS.gene87532.t1 AT5G01210 33.412 425 206 12 66 441 73 469 1.97e-62 209
MS.gene87532.t1 AT3G50280 37.812 320 179 7 127 438 3 310 2.99e-62 204
MS.gene87532.t1 AT5G23940 30.702 456 284 14 4 437 3 448 3.44e-54 188
MS.gene87532.t1 AT3G50290 40.511 274 150 6 122 390 4 269 5.43e-52 176
MS.gene87532.t1 AT5G48930 26.582 395 270 10 43 428 41 424 5.75e-32 126
MS.gene87532.t1 AT3G48720 28.367 349 216 13 55 393 61 385 1.08e-22 100
MS.gene87532.t1 AT5G41040 25.978 358 221 13 52 393 82 411 4.39e-21 95.9
MS.gene87532.t1 AT2G19070 24.835 455 275 20 27 438 19 449 5.28e-21 95.5
MS.gene87532.t1 AT5G41040 25.978 358 221 13 52 393 66 395 5.37e-21 95.5
MS.gene87532.t1 AT3G29670 22.368 456 312 12 1 432 6 443 1.02e-19 91.7
MS.gene87532.t1 AT3G29590 24.945 453 300 16 3 432 7 442 1.17e-19 91.3
MS.gene87532.t1 AT5G63560 26.286 350 218 12 55 393 61 381 3.82e-19 89.7
MS.gene87532.t1 AT5G39080 25.679 405 246 16 52 422 62 445 5.62e-17 83.2
MS.gene87532.t1 AT3G29635 24.936 389 261 12 24 392 29 406 3.62e-16 80.9
MS.gene87532.t1 AT1G24430 23.514 387 249 13 41 414 45 397 3.65e-15 77.8
MS.gene87532.t1 AT2G25150 24.932 365 229 13 52 396 64 403 6.71e-15 77.0
MS.gene87532.t1 AT5G23970 26.471 442 262 23 4 423 3 403 2.06e-14 75.1
MS.gene87532.t1 AT2G40230 26.087 368 243 12 41 397 42 391 3.76e-14 74.3
MS.gene87532.t1 AT5G07080 27.758 281 169 12 56 322 71 331 4.11e-14 74.3
MS.gene87532.t1 AT3G29680 25.063 395 250 15 52 422 59 431 5.64e-14 73.9
MS.gene87532.t1 AT1G28680 22.969 357 224 14 58 395 65 389 6.57e-14 73.9
MS.gene87532.t1 AT5G47980 25.205 365 222 14 54 400 60 391 2.73e-13 72.0
MS.gene87532.t1 AT5G61160 24.727 275 181 10 135 393 139 403 3.45e-13 71.6
MS.gene87532.t1 AT5G16410 24.675 385 236 16 41 399 77 433 9.17e-13 70.5
MS.gene87532.t1 AT1G28680 23.355 304 199 11 58 349 65 346 1.74e-12 69.3
MS.gene87532.t1 AT1G24420 25.364 343 208 14 65 395 75 381 2.47e-12 68.9
MS.gene87532.t1 AT1G24420 25.364 343 208 14 65 395 75 381 2.47e-12 68.9
MS.gene87532.t1 AT1G78990 25.568 352 231 14 53 393 65 396 5.82e-12 67.8
MS.gene87532.t1 AT1G32910 23.596 356 238 13 53 393 63 399 2.18e-11 66.2
MS.gene87532.t1 AT5G39050 23.030 330 211 12 116 415 126 442 4.52e-11 65.1

Find 102 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCATGGTTTGATGTTTA+TGG 0.113342 4.1:+75697526 MS.gene87532:CDS
CCGTTACCAGAAGATTATTT+TGG 0.221902 4.1:+75697295 MS.gene87532:CDS
AATGGATAAGAAATTATTAT+TGG 0.233186 4.1:-75697497 None:intergenic
ACTTGCTTCAACCAACAAAA+AGG 0.235255 4.1:+75696493 MS.gene87532:CDS
CGCAGTGGATATAAAATAAA+TGG 0.235319 4.1:+75697583 MS.gene87532:CDS
AGTGTCTCAGCCGTTGGATT+TGG 0.276668 4.1:-75696547 None:intergenic
TAAGAAATCCAAGCTTTGTT+AGG 0.280992 4.1:+75697461 MS.gene87532:CDS
ATGTCTTCCTTACGAGGTTT+TGG 0.299283 4.1:+75697657 MS.gene87532:CDS
CATACGATTTCCTTTCACTA+TGG 0.304291 4.1:+75697003 MS.gene87532:CDS
GGAGATCCCATGGAGTTAAA+TGG 0.331299 4.1:-75696463 None:intergenic
GATGTTTATGGTAATGATTT+CGG 0.343194 4.1:+75697538 MS.gene87532:CDS
TATCCACTGCGAACTGCTAT+AGG 0.347610 4.1:-75697571 None:intergenic
GTGTCTCAGCCGTTGGATTT+GGG 0.349706 4.1:-75696546 None:intergenic
GAAACCAACGCTCTAGTGTT+GGG 0.360883 4.1:-75696961 None:intergenic
ATTGTATGATCACTTGATTT+TGG 0.375965 4.1:-75696440 None:intergenic
GAGTTTCCAAAATAATCTTC+TGG 0.384510 4.1:-75697301 None:intergenic
GGGAAGTGACCCACATTTCA+TGG 0.400457 4.1:+75697687 MS.gene87532:CDS
ATGGGAAGTTGAATACCATT+TGG 0.405055 4.1:-75696983 None:intergenic
TTCAACCATGTTGTCGTTGA+CGG 0.405415 4.1:+75696863 MS.gene87532:CDS
AAACCATGGAGAACTACTAA+TGG 0.406093 4.1:-75697515 None:intergenic
GGAATTGTAAATGTATTTGC+TGG 0.411545 4.1:+75697604 MS.gene87532:CDS
GCCGACAAGAGGTTGGAAAA+AGG 0.413365 4.1:-75696588 None:intergenic
GATTATATACTTCTTCTTGT+GGG 0.433778 4.1:-75697237 None:intergenic
TGTATAGTCAAAATGAAAGT+TGG 0.440205 4.1:+75697334 MS.gene87532:CDS
TATGGTAATGATTTCGGATG+GGG 0.444025 4.1:+75697544 MS.gene87532:CDS
AAGCGCTATTAACTCACCTT+TGG 0.445268 4.1:+75697185 MS.gene87532:CDS
GACGAAGGTGGATTATATAA+AGG 0.446121 4.1:+75697367 MS.gene87532:CDS
AATGAAAGTTGGTGAGTTGT+TGG 0.448624 4.1:+75697345 MS.gene87532:CDS
TGTGGCATTTCATTAATTCA+TGG 0.451210 4.1:+75696894 MS.gene87532:CDS
AACCTTGAAGCTGCATTAGT+TGG 0.453624 4.1:-75697719 None:intergenic
TGGATTTGGGTTGCTACCAC+CGG 0.462048 4.1:-75696533 None:intergenic
AAGAAATCCAAGCTTTGTTA+GGG 0.462160 4.1:+75697462 MS.gene87532:CDS
GTGGCATTTCATTAATTCAT+GGG 0.463542 4.1:+75696895 MS.gene87532:CDS
TACTTGTGTTGATGACATCT+TGG 0.474749 4.1:+75696706 MS.gene87532:CDS
AGATTATATACTTCTTCTTG+TGG 0.484423 4.1:-75697238 None:intergenic
CCAAAATAATCTTCTGGTAA+CGG 0.486289 4.1:-75697295 None:intergenic
AATCTAGTCGTGATAGGAGT+TGG 0.488221 4.1:+75697256 MS.gene87532:CDS
TGGGTCAAATTGTTTGCAAC+GGG 0.488474 4.1:-75697218 None:intergenic
CTAGCAACCCTAACAAAGCT+TGG 0.494801 4.1:-75697469 None:intergenic
GTATATAATCTAGTCGTGAT+AGG 0.497624 4.1:+75697250 MS.gene87532:CDS
GGAAACCAACGCTCTAGTGT+TGG 0.511281 4.1:-75696962 None:intergenic
TGAAGAATCACTATGAGTCT+TGG 0.513376 4.1:+75697437 MS.gene87532:CDS
CATAGTGAAAGGAAATCGTA+TGG 0.513721 4.1:-75697002 None:intergenic
GGGCCTTCTTTATCACCATC+CGG 0.514663 4.1:+75696514 MS.gene87532:CDS
CTAGAGCGTTGGTTTCCAAA+TGG 0.515960 4.1:+75696968 MS.gene87532:CDS
AGGCACGTCAAAACCATTGT+TGG 0.517049 4.1:+75696793 MS.gene87532:CDS
ACAATTAGAGGAACGTAGAC+CGG 0.517745 4.1:-75696731 None:intergenic
TTTATGGTAATGATTTCGGA+TGG 0.520125 4.1:+75697542 MS.gene87532:CDS
ATACAATCCATTTAACTCCA+TGG 0.520586 4.1:+75696456 MS.gene87532:CDS
CGTAAGGAAGACATACTTGA+AGG 0.521339 4.1:-75697648 None:intergenic
TGTTGATGACATCTTGGGAC+CGG 0.532244 4.1:+75696712 MS.gene87532:CDS
TGTCGGCCGTCTTGAAATAA+CGG 0.534950 4.1:+75696604 MS.gene87532:CDS
TGGTTGAAGCAAGTAGAAAA+CGG 0.535736 4.1:-75696484 None:intergenic
GTGGGTCAAATTGTTTGCAA+CGG 0.538778 4.1:-75697219 None:intergenic
GTCAACGACAACATGGTTGA+AGG 0.541580 4.1:-75696861 None:intergenic
TACAATCCATTTAACTCCAT+GGG 0.542338 4.1:+75696457 MS.gene87532:CDS
TATCCATTAGTAGTTCTCCA+TGG 0.544589 4.1:+75697512 MS.gene87532:CDS
GGTGAGTTGTTGGACGAAGG+TGG 0.550907 4.1:+75697355 MS.gene87532:CDS
ATTTCAAGACGGCCGACAAG+AGG 0.553153 4.1:-75696599 None:intergenic
ATAGAGAGTGTCTCAGCCGT+TGG 0.554838 4.1:-75696553 None:intergenic
ATTGCCTCCAAAACCTCGTA+AGG 0.558716 4.1:-75697664 None:intergenic
TTATGGTAATGATTTCGGAT+GGG 0.560500 4.1:+75697543 MS.gene87532:CDS
CAAGACGGCCGACAAGAGGT+TGG 0.562878 4.1:-75696595 None:intergenic
AACAAAATAGCCATTGTGAT+GGG 0.565478 4.1:+75697029 MS.gene87532:CDS
GTCACGACATCCATGAAATG+TGG 0.565781 4.1:-75697697 None:intergenic
ACTTGTGTTGATGACATCTT+GGG 0.567798 4.1:+75696707 MS.gene87532:CDS
GAAATCGAAAATCAATGAAG+AGG 0.568871 4.1:+75697135 MS.gene87532:CDS
AAACCAACGCTCTAGTGTTG+GGG 0.569521 4.1:-75696960 None:intergenic
GTATTTGCTGGAATAGAAGA+AGG 0.570455 4.1:+75697616 MS.gene87532:CDS
CAACAAAATAGCCATTGTGA+TGG 0.573773 4.1:+75697028 MS.gene87532:CDS
TTGTATCAGTGGAGAGAACT+CGG 0.576741 4.1:-75696409 None:intergenic
TCTCTCCACTGATACAATCA+AGG 0.580251 4.1:+75696415 MS.gene87532:CDS
ACTTTCCGTCAACGACAACA+TGG 0.580850 4.1:-75696868 None:intergenic
AGTAGAAAACGGAGATCCCA+TGG 0.582005 4.1:-75696473 None:intergenic
TCAAGTATGTCTTCCTTACG+AGG 0.584114 4.1:+75697651 MS.gene87532:CDS
CAAGTTAATTGCTTCACACA+CGG 0.586291 4.1:+75697405 MS.gene87532:CDS
CAAGTAACGGAGCTAGTTGA+CGG 0.586570 4.1:+75696821 MS.gene87532:CDS
TGTGTTCCGTTATTTCAAGA+CGG 0.586751 4.1:-75696610 None:intergenic
TGGTGCCTTGATTGTATCAG+TGG 0.587630 4.1:-75696420 None:intergenic
ATTGTTGGCTGTTCAAGTAA+CGG 0.588367 4.1:+75696808 MS.gene87532:CDS
CCACCGGATGGTGATAAAGA+AGG 0.588719 4.1:-75696517 None:intergenic
GTTGGTGAGTTGTTGGACGA+AGG 0.590545 4.1:+75697352 MS.gene87532:CDS
GAAATATCACGTGGTTGTTG+TGG 0.597765 4.1:+75696920 MS.gene87532:CDS
GGTAACGGTGGAACAAACCT+TGG 0.603404 4.1:-75697280 None:intergenic
AACGGGTGACAAAGCACCAA+AGG 0.603630 4.1:-75697201 None:intergenic
TAGGTTATTTCATTTCACAA+AGG 0.626286 4.1:+75697096 MS.gene87532:CDS
AACCAACGCTCTAGTGTTGG+GGG 0.632370 4.1:-75696959 None:intergenic
GTTACTTGAACAGCCAACAA+TGG 0.633211 4.1:-75696806 None:intergenic
ATAGTGAAAGGAAATCGTAT+GGG 0.637742 4.1:-75697001 None:intergenic
TTTGGGTTGCTACCACCGGA+TGG 0.640724 4.1:-75696529 None:intergenic
AAATAATCTTCTGGTAACGG+TGG 0.643941 4.1:-75697292 None:intergenic
TGGTAGCAACCCAAATCCAA+CGG 0.644473 4.1:+75696537 MS.gene87532:CDS
TCACGACATCCATGAAATGT+GGG 0.644749 4.1:-75697696 None:intergenic
TCGTGATAGGAGTTGGACCA+AGG 0.645786 4.1:+75697263 MS.gene87532:CDS
TTACCATAAACATCAAACCA+TGG 0.659822 4.1:-75697529 None:intergenic
TCTGTTATATGCAACAATGC+AGG 0.665996 4.1:+75696653 MS.gene87532:CDS
CCTTCTTTATCACCATCCGG+TGG 0.673821 4.1:+75696517 MS.gene87532:CDS
TGAATTAATGAAATGCCACA+CGG 0.688433 4.1:-75696891 None:intergenic
AAGTATTCTCTGCTGCAGCG+TGG 0.693165 4.1:-75696688 None:intergenic
AAACCTATAGCAGTTCGCAG+TGG 0.705186 4.1:+75697568 MS.gene87532:CDS
TCATGGGCTGAAATATCACG+TGG 0.731649 4.1:+75696911 MS.gene87532:CDS
TCGTTGACGGAAAGTCCGTG+TGG 0.742237 4.1:+75696876 MS.gene87532:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGGATAAGAAATTATTAT+TGG - chr4.1:75697500-75697519 None:intergenic 15.0%
!!! AAAAACTTTGTTTATCAAAT+AGG + chr4.1:75697077-75697096 MS.gene87532:CDS 15.0%
! AAAAATGAATCGACAATTAG+AGG - chr4.1:75696746-75696765 None:intergenic 25.0%
! AGATTATATACTTCTTCTTG+TGG - chr4.1:75697241-75697260 None:intergenic 25.0%
! GATTATATACTTCTTCTTGT+GGG - chr4.1:75697240-75697259 None:intergenic 25.0%
! TGTATAGTCAAAATGAAAGT+TGG + chr4.1:75697334-75697353 MS.gene87532:CDS 25.0%
!! ATAGTTTTTATCTCCGATAA+AGG - chr4.1:75696771-75696790 None:intergenic 25.0%
!! ATTGTATGATCACTTGATTT+TGG - chr4.1:75696443-75696462 None:intergenic 25.0%
!! GATGTTTATGGTAATGATTT+CGG + chr4.1:75697538-75697557 MS.gene87532:CDS 25.0%
!! TAGGTTATTTCATTTCACAA+AGG + chr4.1:75697096-75697115 MS.gene87532:CDS 25.0%
!!! GATTCATTTTTTGCCTTTAT+CGG + chr4.1:75696755-75696774 MS.gene87532:CDS 25.0%
AACAAAATAGCCATTGTGAT+GGG + chr4.1:75697029-75697048 MS.gene87532:CDS 30.0%
ATACAATCCATTTAACTCCA+TGG + chr4.1:75696456-75696475 MS.gene87532:CDS 30.0%
ATAGTGAAAGGAAATCGTAT+GGG - chr4.1:75697004-75697023 None:intergenic 30.0%
ATCGGAGATAAAAACTATGA+AGG + chr4.1:75696773-75696792 MS.gene87532:CDS 30.0%
CCAAAATAATCTTCTGGTAA+CGG - chr4.1:75697298-75697317 None:intergenic 30.0%
CGCAGTGGATATAAAATAAA+TGG + chr4.1:75697583-75697602 MS.gene87532:CDS 30.0%
GAAATCGAAAATCAATGAAG+AGG + chr4.1:75697135-75697154 MS.gene87532:CDS 30.0%
GAGTTTCCAAAATAATCTTC+TGG - chr4.1:75697304-75697323 None:intergenic 30.0%
GGAATTGTAAATGTATTTGC+TGG + chr4.1:75697604-75697623 MS.gene87532:CDS 30.0%
GTATATAATCTAGTCGTGAT+AGG + chr4.1:75697250-75697269 MS.gene87532:CDS 30.0%
GTGGCATTTCATTAATTCAT+GGG + chr4.1:75696895-75696914 MS.gene87532:CDS 30.0%
TACAATCCATTTAACTCCAT+GGG + chr4.1:75696457-75696476 MS.gene87532:CDS 30.0%
TGAATTAATGAAATGCCACA+CGG - chr4.1:75696894-75696913 None:intergenic 30.0%
TGTGGCATTTCATTAATTCA+TGG + chr4.1:75696894-75696913 MS.gene87532:CDS 30.0%
TTACCATAAACATCAAACCA+TGG - chr4.1:75697532-75697551 None:intergenic 30.0%
! TTATGGTAATGATTTCGGAT+GGG + chr4.1:75697543-75697562 MS.gene87532:CDS 30.0%
! TTTATGGTAATGATTTCGGA+TGG + chr4.1:75697542-75697561 MS.gene87532:CDS 30.0%
!! AAGAAATCCAAGCTTTGTTA+GGG + chr4.1:75697462-75697481 MS.gene87532:CDS 30.0%
!! TAAGAAATCCAAGCTTTGTT+AGG + chr4.1:75697461-75697480 MS.gene87532:CDS 30.0%
!!! TTTTGTTGTTCCATAGTGAA+AGG - chr4.1:75697016-75697035 None:intergenic 30.0%
AAAAACTCAACCCATCACAA+TGG - chr4.1:75697042-75697061 None:intergenic 35.0%
AAACCATGGAGAACTACTAA+TGG - chr4.1:75697518-75697537 None:intergenic 35.0%
AAGAGGTTGGAAAAAGGAAA+GGG - chr4.1:75696585-75696604 None:intergenic 35.0%
ACTTGCTTCAACCAACAAAA+AGG + chr4.1:75696493-75696512 MS.gene87532:CDS 35.0%
ATGGGAAGTTGAATACCATT+TGG - chr4.1:75696986-75697005 None:intergenic 35.0%
CAACAAAATAGCCATTGTGA+TGG + chr4.1:75697028-75697047 MS.gene87532:CDS 35.0%
CAAGTTAATTGCTTCACACA+CGG + chr4.1:75697405-75697424 MS.gene87532:CDS 35.0%
CATACGATTTCCTTTCACTA+TGG + chr4.1:75697003-75697022 MS.gene87532:CDS 35.0%
CATAGTGAAAGGAAATCGTA+TGG - chr4.1:75697005-75697024 None:intergenic 35.0%
CTTGCTTCAACCAACAAAAA+GGG + chr4.1:75696494-75696513 MS.gene87532:CDS 35.0%
TATCCATTAGTAGTTCTCCA+TGG + chr4.1:75697512-75697531 MS.gene87532:CDS 35.0%
TCTCCATGGTTTGATGTTTA+TGG + chr4.1:75697526-75697545 MS.gene87532:CDS 35.0%
TCTGTTATATGCAACAATGC+AGG + chr4.1:75696653-75696672 MS.gene87532:CDS 35.0%
TGAAGAATCACTATGAGTCT+TGG + chr4.1:75697437-75697456 MS.gene87532:CDS 35.0%
TGGTTGAAGCAAGTAGAAAA+CGG - chr4.1:75696487-75696506 None:intergenic 35.0%
! ATAAAGAAGGCCCTTTTTGT+TGG - chr4.1:75696507-75696526 None:intergenic 35.0%
! ATTGTTGGCTGTTCAAGTAA+CGG + chr4.1:75696808-75696827 MS.gene87532:CDS 35.0%
! CCGTTACCAGAAGATTATTT+TGG + chr4.1:75697295-75697314 MS.gene87532:CDS 35.0%
! CTAGTTGACGGTATTTTCAT+TGG + chr4.1:75696833-75696852 MS.gene87532:CDS 35.0%
! GTATTTGCTGGAATAGAAGA+AGG + chr4.1:75697616-75697635 MS.gene87532:CDS 35.0%
! TATGGTAATGATTTCGGATG+GGG + chr4.1:75697544-75697563 MS.gene87532:CDS 35.0%
! TGTGTTCCGTTATTTCAAGA+CGG - chr4.1:75696613-75696632 None:intergenic 35.0%
!! AAATAATCTTCTGGTAACGG+TGG - chr4.1:75697295-75697314 None:intergenic 35.0%
!! AATGAAAGTTGGTGAGTTGT+TGG + chr4.1:75697345-75697364 MS.gene87532:CDS 35.0%
!! ACTTGTGTTGATGACATCTT+GGG + chr4.1:75696707-75696726 MS.gene87532:CDS 35.0%
!! GACGAAGGTGGATTATATAA+AGG + chr4.1:75697367-75697386 MS.gene87532:CDS 35.0%
!! TACTTGTGTTGATGACATCT+TGG + chr4.1:75696706-75696725 MS.gene87532:CDS 35.0%
AAGCGCTATTAACTCACCTT+TGG + chr4.1:75697185-75697204 MS.gene87532:CDS 40.0%
AATCTAGTCGTGATAGGAGT+TGG + chr4.1:75697256-75697275 MS.gene87532:CDS 40.0%
ACAATTAGAGGAACGTAGAC+CGG - chr4.1:75696734-75696753 None:intergenic 40.0%
CAAGAGGTTGGAAAAAGGAA+AGG - chr4.1:75696586-75696605 None:intergenic 40.0%
CGTAAGGAAGACATACTTGA+AGG - chr4.1:75697651-75697670 None:intergenic 40.0%
GAAATATCACGTGGTTGTTG+TGG + chr4.1:75696920-75696939 MS.gene87532:CDS 40.0%
GTGGGTCAAATTGTTTGCAA+CGG - chr4.1:75697222-75697241 None:intergenic 40.0%
GTTACTTGAACAGCCAACAA+TGG - chr4.1:75696809-75696828 None:intergenic 40.0%
TCAAGTATGTCTTCCTTACG+AGG + chr4.1:75697651-75697670 MS.gene87532:CDS 40.0%
TCACGACATCCATGAAATGT+GGG - chr4.1:75697699-75697718 None:intergenic 40.0%
TCTCTCCACTGATACAATCA+AGG + chr4.1:75696415-75696434 MS.gene87532:CDS 40.0%
TGGGTCAAATTGTTTGCAAC+GGG - chr4.1:75697221-75697240 None:intergenic 40.0%
TTCAACCATGTTGTCGTTGA+CGG + chr4.1:75696863-75696882 MS.gene87532:CDS 40.0%
TTGTATCAGTGGAGAGAACT+CGG - chr4.1:75696412-75696431 None:intergenic 40.0%
! ATGTCTTCCTTACGAGGTTT+TGG + chr4.1:75697657-75697676 MS.gene87532:CDS 40.0%
! TCCTTTTTCCAACCTCTTGT+CGG + chr4.1:75696587-75696606 MS.gene87532:CDS 40.0%
!! GAGTTTTTCAGCGTCTAATG+AGG + chr4.1:75697054-75697073 MS.gene87532:CDS 40.0%
AAACCAACGCTCTAGTGTTG+GGG - chr4.1:75696963-75696982 None:intergenic 45.0%
AAACCTATAGCAGTTCGCAG+TGG + chr4.1:75697568-75697587 MS.gene87532:CDS 45.0%
ACTTTCCGTCAACGACAACA+TGG - chr4.1:75696871-75696890 None:intergenic 45.0%
AGGCACGTCAAAACCATTGT+TGG + chr4.1:75696793-75696812 MS.gene87532:CDS 45.0%
AGTAGAAAACGGAGATCCCA+TGG - chr4.1:75696476-75696495 None:intergenic 45.0%
ATTGCCTCCAAAACCTCGTA+AGG - chr4.1:75697667-75697686 None:intergenic 45.0%
CTAGCAACCCTAACAAAGCT+TGG - chr4.1:75697472-75697491 None:intergenic 45.0%
GAAACCAACGCTCTAGTGTT+GGG - chr4.1:75696964-75696983 None:intergenic 45.0%
GGAGATCCCATGGAGTTAAA+TGG - chr4.1:75696466-75696485 None:intergenic 45.0%
GTCAACGACAACATGGTTGA+AGG - chr4.1:75696864-75696883 None:intergenic 45.0%
GTCACGACATCCATGAAATG+TGG - chr4.1:75697700-75697719 None:intergenic 45.0%
TATCCACTGCGAACTGCTAT+AGG - chr4.1:75697574-75697593 None:intergenic 45.0%
TGGTAGCAACCCAAATCCAA+CGG + chr4.1:75696537-75696556 MS.gene87532:CDS 45.0%
TGGTGCCTTGATTGTATCAG+TGG - chr4.1:75696423-75696442 None:intergenic 45.0%
TGTCGGCCGTCTTGAAATAA+CGG + chr4.1:75696604-75696623 MS.gene87532:CDS 45.0%
TTGGAAAAAGGAAAGGGCAG+AGG - chr4.1:75696579-75696598 None:intergenic 45.0%
! CAAGTAACGGAGCTAGTTGA+CGG + chr4.1:75696821-75696840 MS.gene87532:CDS 45.0%
! TCATGGGCTGAAATATCACG+TGG + chr4.1:75696911-75696930 MS.gene87532:CDS 45.0%
!! CTAGAGCGTTGGTTTCCAAA+TGG + chr4.1:75696968-75696987 MS.gene87532:CDS 45.0%
!! TCTTCCTTACGAGGTTTTGG+AGG + chr4.1:75697660-75697679 MS.gene87532:CDS 45.0%
!! TGTTGATGACATCTTGGGAC+CGG + chr4.1:75696712-75696731 MS.gene87532:CDS 45.0%
!!! TACGAGGTTTTGGAGGCAAT+GGG + chr4.1:75697667-75697686 MS.gene87532:CDS 45.0%
!!! TTACGAGGTTTTGGAGGCAA+TGG + chr4.1:75697666-75697685 MS.gene87532:CDS 45.0%
AAGTATTCTCTGCTGCAGCG+TGG - chr4.1:75696691-75696710 None:intergenic 50.0%
AGTGTCTCAGCCGTTGGATT+TGG - chr4.1:75696550-75696569 None:intergenic 50.0%
ATTTCAAGACGGCCGACAAG+AGG - chr4.1:75696602-75696621 None:intergenic 50.0%
CCACCGGATGGTGATAAAGA+AGG - chr4.1:75696520-75696539 None:intergenic 50.0%
CCTTCTTTATCACCATCCGG+TGG + chr4.1:75696517-75696536 MS.gene87532:CDS 50.0%
GCCGACAAGAGGTTGGAAAA+AGG - chr4.1:75696591-75696610 None:intergenic 50.0%
GGAAACCAACGCTCTAGTGT+TGG - chr4.1:75696965-75696984 None:intergenic 50.0%
GGGAAGTGACCCACATTTCA+TGG + chr4.1:75697687-75697706 MS.gene87532:CDS 50.0%
GGGCCTTCTTTATCACCATC+CGG + chr4.1:75696514-75696533 MS.gene87532:CDS 50.0%
GTGTCTCAGCCGTTGGATTT+GGG - chr4.1:75696549-75696568 None:intergenic 50.0%
TCGTGATAGGAGTTGGACCA+AGG + chr4.1:75697263-75697282 MS.gene87532:CDS 50.0%
! AACCAACGCTCTAGTGTTGG+GGG - chr4.1:75696962-75696981 None:intergenic 50.0%
! AACGGGTGACAAAGCACCAA+AGG - chr4.1:75697204-75697223 None:intergenic 50.0%
! GGTAACGGTGGAACAAACCT+TGG - chr4.1:75697283-75697302 None:intergenic 50.0%
! GTTGGTGAGTTGTTGGACGA+AGG + chr4.1:75697352-75697371 MS.gene87532:CDS 50.0%
!! ATAGAGAGTGTCTCAGCCGT+TGG - chr4.1:75696556-75696575 None:intergenic 50.0%
!! TGGATTTGGGTTGCTACCAC+CGG - chr4.1:75696536-75696555 None:intergenic 50.0%
AACCCCCAACACTAGAGCGT+TGG + chr4.1:75696957-75696976 MS.gene87532:CDS 55.0%
GGTGAGTTGTTGGACGAAGG+TGG + chr4.1:75697355-75697374 MS.gene87532:CDS 55.0%
TCGTTGACGGAAAGTCCGTG+TGG + chr4.1:75696876-75696895 MS.gene87532:CDS 55.0%
! ACGCTCTAGTGTTGGGGGTT+TGG - chr4.1:75696957-75696976 None:intergenic 55.0%
!! TTTGGGTTGCTACCACCGGA+TGG - chr4.1:75696532-75696551 None:intergenic 55.0%
CAAGACGGCCGACAAGAGGT+TGG - chr4.1:75696598-75696617 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 75696398 75697726 75696398 ID=MS.gene87532
chr4.1 mRNA 75696398 75697726 75696398 ID=MS.gene87532.t1;Parent=MS.gene87532
chr4.1 exon 75696398 75697726 75696398 ID=MS.gene87532.t1.exon1;Parent=MS.gene87532.t1
chr4.1 CDS 75696398 75697726 75696398 ID=cds.MS.gene87532.t1;Parent=MS.gene87532.t1
Gene Sequence

>MS.gene87532

ATGGATGTCGTCCGAGTTCTCTCCACTGATACAATCAAGGCACCAAAATCAAGTGATCATACAATCCATTTAACTCCATGGGATCTCCGTTTTCTACTTGCTTCAACCAACAAAAAGGGCCTTCTTTATCACCATCCGGTGGTAGCAACCCAAATCCAACGGCTGAGACACTCTCTATCCTCTGCCCTTTCCTTTTTCCAACCTCTTGTCGGCCGTCTTGAAATAACGGAACACAAAGACAATATTGTCTCGTGTTCTGTTATATGCAACAATGCAGGTGTACTCTTTATCCACGCTGCAGCAGAGAATACTTGTGTTGATGACATCTTGGGACCGGTCTACGTTCCTCTAATTGTCGATTCATTTTTTGCCTTTATCGGAGATAAAAACTATGAAGGCACGTCAAAACCATTGTTGGCTGTTCAAGTAACGGAGCTAGTTGACGGTATTTTCATTGGTTGCTCCTTCAACCATGTTGTCGTTGACGGAAAGTCCGTGTGGCATTTCATTAATTCATGGGCTGAAATATCACGTGGTTGTTGTGGTGATCAAATATCCAAACCCCCAACACTAGAGCGTTGGTTTCCAAATGGTATTCAACTTCCCATACGATTTCCTTTCACTATGGAACAACAAAATAGCCATTGTGATGGGTTGAGTTTTTCAGCGTCTAATGAGGAAAAACTTTGTTTATCAAATAGGTTATTTCATTTCACAAAGGAGAAAATCATTCAACTGAAATCGAAAATCAATGAAGAGGTTAGTACAATCAAAATATCATCTCTACAAGCGCTATTAACTCACCTTTGGTGCTTTGTCACCCGTTGCAAACAATTTGACCCACAAGAAGAAGTATATAATCTAGTCGTGATAGGAGTTGGACCAAGGTTTGTTCCACCGTTACCAGAAGATTATTTTGGAAACTCGATTATAAGTTGTATAGTCAAAATGAAAGTTGGTGAGTTGTTGGACGAAGGTGGATTATATAAAGGTGCTTGTGAGATGAACAAGTTAATTGCTTCACACACGGATGAGAAGTTGAAGAATCACTATGAGTCTTGGCTAAGAAATCCAAGCTTTGTTAGGGTTGCTAGCATGTCCAATAATAATTTCTTATCCATTAGTAGTTCTCCATGGTTTGATGTTTATGGTAATGATTTCGGATGGGGAAAACCTATAGCAGTTCGCAGTGGATATAAAATAAATGGAATTGTAAATGTATTTGCTGGAATAGAAGAAGGTAGTATTGACCTTCAAGTATGTCTTCCTTACGAGGTTTTGGAGGCAATGGGAAGTGACCCACATTTCATGGATGTCGTGACCAACTAA
Protein sequence

>MS.gene87532.t1

MDVVRVLSTDTIKAPKSSDHTIHLTPWDLRFLLASTNKKGLLYHHPVVATQIQRLRHSLSSALSFFQPLVGRLEITEHKDNIVSCSVICNNAGVLFIHAAAENTCVDDILGPVYVPLIVDSFFAFIGDKNYEGTSKPLLAVQVTELVDGIFIGCSFNHVVVDGKSVWHFINSWAEISRGCCGDQISKPPTLERWFPNGIQLPIRFPFTMEQQNSHCDGLSFSASNEEKLCLSNRLFHFTKEKIIQLKSKINEEVSTIKISSLQALLTHLWCFVTRCKQFDPQEEVYNLVVIGVGPRFVPPLPEDYFGNSIISCIVKMKVGELLDEGGLYKGACEMNKLIASHTDEKLKNHYESWLRNPSFVRVASMSNNNFLSISSSPWFDVYGNDFGWGKPIAVRSGYKINGIVNVFAGIEEGSIDLQVCLPYEVLEAMGSDPHFMDVVTN