AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50356

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50356.t1 MTR_4g022670 90.950 442 40 0 1 442 1 442 0.0 835
MS.gene50356.t1 MTR_4g022800 82.844 443 75 1 1 442 1 443 0.0 768
MS.gene50356.t1 MTR_4g022720 81.900 442 74 1 1 442 1 436 0.0 759
MS.gene50356.t1 MTR_7g026860 80.090 442 85 1 1 442 1 439 0.0 741
MS.gene50356.t1 MTR_4g022825 75.909 440 105 1 1 440 1 439 0.0 696
MS.gene50356.t1 MTR_4g022760 76.018 442 62 1 1 442 1 398 0.0 682
MS.gene50356.t1 MTR_3g088420 67.873 442 134 2 1 442 1 434 0.0 613
MS.gene50356.t1 MTR_3g026310 65.479 449 148 2 1 442 1 449 0.0 597
MS.gene50356.t1 MTR_8g041770 58.720 453 165 6 1 442 1 442 0.0 514
MS.gene50356.t1 MTR_0190s0020 57.301 452 172 7 1 441 1 442 0.0 513
MS.gene50356.t1 MTR_4g094842 56.674 457 169 10 1 439 1 446 2.15e-168 481
MS.gene50356.t1 MTR_4g094835 53.626 455 180 8 1 437 1 442 1.61e-163 468
MS.gene50356.t1 MTR_4g022290 52.495 461 179 12 1 439 1 443 3.30e-151 437
MS.gene50356.t1 MTR_4g094828 51.747 458 196 11 1 441 1 450 3.20e-147 427
MS.gene50356.t1 MTR_6g016815 53.139 446 127 9 1 442 15 382 9.80e-147 423
MS.gene50356.t1 MTR_4g094838 49.234 457 180 10 4 437 1 428 5.03e-145 421
MS.gene50356.t1 MTR_8g079710 50.889 450 189 10 1 432 1 436 1.53e-143 417
MS.gene50356.t1 MTR_4g094832 55.091 383 145 6 69 437 57 426 6.78e-137 400
MS.gene50356.t1 MTR_3g101900 39.498 438 238 11 18 441 24 448 8.71e-95 293
MS.gene50356.t1 MTR_3g101930 39.013 446 238 11 16 440 22 454 1.13e-92 288
MS.gene50356.t1 MTR_7g026850 75.449 167 41 0 29 195 12 178 5.52e-90 271
MS.gene50356.t1 MTR_8g040760 64.186 215 70 3 1 209 1 214 4.11e-89 270
MS.gene50356.t1 MTR_3g102020 38.952 439 241 10 16 438 21 448 1.79e-88 277
MS.gene50356.t1 MTR_7g026770 67.677 198 36 1 141 338 132 301 5.53e-87 268
MS.gene50356.t1 MTR_3g101960 38.532 436 242 9 16 436 21 445 4.46e-86 270
MS.gene50356.t1 MTR_3g101970 37.585 439 237 10 16 440 21 436 8.00e-84 264
MS.gene50356.t1 MTR_3g102010 36.961 441 252 10 16 441 22 451 9.06e-82 259
MS.gene50356.t1 MTR_8g040780 58.525 217 86 2 224 436 11 227 3.09e-81 251
MS.gene50356.t1 MTR_4g015180 32.924 489 247 15 16 442 19 488 5.04e-59 201
MS.gene50356.t1 MTR_3g070170 31.533 463 255 12 16 437 25 466 3.87e-57 196
MS.gene50356.t1 MTR_4g094825 52.910 189 80 5 17 199 84 269 1.17e-54 187
MS.gene50356.t1 MTR_4g094825 50.649 154 63 2 286 437 245 387 1.35e-44 160
MS.gene50356.t1 MTR_5g081960 30.526 475 274 12 16 441 19 486 1.30e-54 189
MS.gene50356.t1 MTR_4g022780 52.941 187 54 6 200 382 7 163 8.25e-50 167
MS.gene50356.t1 MTR_7g026800 56.140 171 44 4 212 380 19 160 9.22e-50 167
MS.gene50356.t1 MTR_3g102030 35.161 310 162 5 90 397 48 320 3.99e-49 170
MS.gene50356.t1 MTR_6g034495 56.129 155 59 3 33 179 8 161 4.21e-48 162
MS.gene50356.t1 MTR_6g034485 62.879 132 41 2 189 320 2 125 2.32e-47 159
MS.gene50356.t1 MTR_6g045553 29.263 475 278 15 4 437 14 471 9.19e-46 165
MS.gene50356.t1 MTR_7g026830 80.952 84 16 0 83 166 15 98 3.88e-42 144
MS.gene50356.t1 MTR_7g026780 61.261 111 19 1 332 442 26 112 2.86e-39 137
MS.gene50356.t1 MTR_7g026870 100.000 55 0 0 297 351 97 151 2.89e-32 120
MS.gene50356.t1 MTR_4g007540 26.757 441 267 15 16 428 12 424 6.50e-32 126
MS.gene50356.t1 MTR_8g075610 26.649 379 259 10 55 428 47 411 5.06e-31 124
MS.gene50356.t1 MTR_2g064495 27.013 385 253 11 55 428 56 423 8.17e-31 123
MS.gene50356.t1 MTR_2g105330 27.368 380 248 11 55 423 56 418 2.43e-30 122
MS.gene50356.t1 MTR_8g096530 27.361 413 250 15 50 436 54 442 4.32e-26 110
MS.gene50356.t1 MTR_7g026880 88.462 52 6 0 390 441 2 53 6.64e-26 99.8
MS.gene50356.t1 MTR_3g098980 27.019 359 207 13 55 393 66 389 1.66e-22 99.8
MS.gene50356.t1 MTR_4g013050 26.944 360 197 14 53 393 51 363 1.98e-21 96.3
MS.gene50356.t1 MTR_3g026950 25.310 403 257 14 52 426 51 437 1.40e-20 94.0
MS.gene50356.t1 MTR_3g049150 25.935 401 252 16 52 426 52 433 6.30e-20 92.0
MS.gene50356.t1 MTR_7g014330 24.272 412 261 11 18 396 20 413 8.10e-20 92.0
MS.gene50356.t1 MTR_7g027175 25.459 381 258 10 55 428 68 429 1.77e-19 90.5
MS.gene50356.t1 MTR_7g014250 24.213 413 258 12 19 396 21 413 4.39e-19 89.7
MS.gene50356.t1 MTR_3g086020 26.711 453 275 19 4 441 5 415 5.33e-18 85.9
MS.gene50356.t1 MTR_2g089765 23.583 441 265 16 3 392 15 434 8.24e-18 85.9
MS.gene50356.t1 MTR_7g014230 25.182 413 256 13 18 396 22 415 8.81e-18 85.9
MS.gene50356.t1 MTR_6g015315 27.273 275 176 8 138 395 140 407 1.05e-17 85.5
MS.gene50356.t1 MTR_5g076700 23.624 436 284 16 25 433 24 437 1.16e-17 85.5
MS.gene50356.t1 MTR_2g089670 24.737 380 251 14 38 392 60 429 2.49e-17 84.3
MS.gene50356.t1 MTR_3g087640 24.947 473 303 17 1 432 5 466 3.31e-17 84.0
MS.gene50356.t1 MTR_2g089650 24.096 415 267 15 38 418 43 443 3.45e-17 84.0
MS.gene50356.t1 MTR_1g040175 26.259 278 189 9 50 324 59 323 4.08e-17 83.6
MS.gene50356.t1 MTR_2g089755 23.198 444 295 14 24 432 37 469 6.03e-17 83.2
MS.gene50356.t1 MTR_7g114490 24.612 451 272 21 25 439 24 442 7.40e-17 83.2
MS.gene50356.t1 MTR_6g015830 25.109 458 284 15 10 432 21 454 1.13e-16 82.4
MS.gene50356.t1 MTR_1g081660 24.194 310 186 10 24 308 31 316 1.74e-16 81.6
MS.gene50356.t1 MTR_3g028210 24.459 462 287 19 4 423 8 449 2.91e-16 81.3
MS.gene50356.t1 MTR_7g015860 23.341 437 260 16 4 393 5 413 4.77e-16 80.5
MS.gene50356.t1 MTR_7g114560 24.283 453 259 20 25 433 24 436 9.87e-16 79.3
MS.gene50356.t1 MTR_4g097090 22.788 373 235 11 55 398 65 413 1.47e-15 79.0
MS.gene50356.t1 MTR_6g015850 25.559 313 207 9 138 432 147 451 1.66e-15 78.6
MS.gene50356.t1 MTR_6g015320 24.671 304 191 9 138 414 143 435 2.00e-15 78.6
MS.gene50356.t1 MTR_3g112180 24.356 427 291 14 11 428 23 426 3.15e-15 77.8
MS.gene50356.t1 MTR_3g071320 25.602 332 216 10 22 334 24 343 7.20e-15 76.6
MS.gene50356.t1 MTR_5g090880 23.990 421 266 15 55 441 67 467 1.48e-14 75.9
MS.gene50356.t1 MTR_1g040315 27.000 300 186 11 25 309 23 304 1.49e-14 75.5
MS.gene50356.t1 MTR_7g056087 22.449 392 268 13 54 428 57 429 3.17e-14 74.7
MS.gene50356.t1 MTR_7g114520 26.374 273 180 11 44 308 53 312 5.78e-14 73.9
MS.gene50356.t1 MTR_1g040215 25.128 390 246 15 25 393 23 387 5.97e-14 73.9
MS.gene50356.t1 MTR_4g127650 23.785 391 259 12 55 422 66 440 6.63e-14 73.9
MS.gene50356.t1 MTR_2g089735 24.362 431 269 18 41 432 43 455 1.58e-13 72.8
MS.gene50356.t1 MTR_1g040320 24.377 361 202 14 44 393 51 351 1.63e-13 72.4
MS.gene50356.t1 MTR_7g084940 26.923 286 171 12 53 322 67 330 2.02e-13 72.4
MS.gene50356.t1 MTR_1g053315 24.309 362 222 17 55 393 78 410 2.19e-13 72.4
MS.gene50356.t1 MTR_5g084870 23.245 413 263 16 4 397 3 380 3.79e-13 71.2
MS.gene50356.t1 MTR_7g084910 25.788 349 199 18 32 341 36 363 7.63e-13 70.5
MS.gene50356.t1 MTR_7g014200 24.828 435 245 18 15 396 19 424 9.71e-13 70.1
MS.gene50356.t1 MTR_3g465910 27.491 291 170 12 57 330 70 336 1.14e-12 70.1
MS.gene50356.t1 MTR_4g055170 26.471 306 197 11 55 351 69 355 1.33e-12 69.7
MS.gene50356.t1 MTR_2g015430 24.444 405 250 17 14 395 15 386 1.40e-12 69.7
MS.gene50356.t1 MTR_3g465930 26.910 301 179 11 55 339 69 344 1.78e-12 69.3
MS.gene50356.t1 MTR_7g014160 24.078 461 273 17 15 422 19 455 1.80e-12 69.3
MS.gene50356.t1 MTR_5g090830 24.397 373 222 14 55 393 66 412 2.22e-12 68.9
MS.gene50356.t1 MTR_7g085930 22.564 390 255 12 43 399 47 422 5.33e-12 67.8
MS.gene50356.t1 MTR_3g049140 27.513 189 119 5 226 399 18 203 5.48e-12 66.2
MS.gene50356.t1 MTR_0502s0010 28.856 201 120 8 134 325 39 225 6.06e-11 62.8
MS.gene50356.t1 MTR_7g014360 25.993 277 164 10 138 396 90 343 7.66e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50356.t1 AT5G67150 39.514 453 247 11 1 437 1 442 5.23e-95 293
MS.gene50356.t1 AT5G67160 39.414 444 238 12 6 441 12 432 2.84e-92 286
MS.gene50356.t1 AT3G50300 39.954 438 233 13 1 422 1 424 3.71e-90 281
MS.gene50356.t1 AT3G50270 38.938 452 250 12 6 441 7 448 1.82e-88 276
MS.gene50356.t1 AT3G50280 37.111 450 261 10 1 438 1 440 2.72e-88 276
MS.gene50356.t1 AT5G42830 37.188 441 251 11 15 441 21 449 3.01e-83 263
MS.gene50356.t1 AT5G38130 37.844 436 243 12 1 422 21 442 4.90e-80 255
MS.gene50356.t1 AT5G07860 36.819 459 250 13 4 440 8 448 2.62e-79 253
MS.gene50356.t1 AT5G07850 38.427 445 245 13 15 442 22 454 9.18e-78 249
MS.gene50356.t1 AT5G07870 36.853 464 259 13 4 442 8 462 2.48e-74 240
MS.gene50356.t1 AT2G39980 32.851 484 245 15 16 442 21 481 4.76e-67 222
MS.gene50356.t1 AT3G50280 38.050 318 181 6 127 438 3 310 6.78e-64 209
MS.gene50356.t1 AT5G01210 32.839 472 250 13 16 441 19 469 1.44e-61 207
MS.gene50356.t1 AT3G50290 41.455 275 148 6 122 391 4 270 3.47e-54 182
MS.gene50356.t1 AT5G23940 29.956 454 290 13 4 437 3 448 1.46e-50 178
MS.gene50356.t1 AT5G48930 27.451 408 250 13 43 428 41 424 6.41e-32 126
MS.gene50356.t1 AT5G41040 26.685 356 225 13 50 393 64 395 1.81e-22 99.8
MS.gene50356.t1 AT5G41040 26.685 356 225 13 50 393 80 411 2.31e-22 99.4
MS.gene50356.t1 AT5G57840 25.517 435 280 17 11 422 9 422 3.71e-22 99.0
MS.gene50356.t1 AT3G48720 27.869 366 226 14 42 393 44 385 3.71e-22 98.6
MS.gene50356.t1 AT3G29590 25.277 451 301 16 3 432 7 442 1.71e-21 97.1
MS.gene50356.t1 AT3G29670 22.955 440 305 13 3 422 8 433 3.01e-20 93.2
MS.gene50356.t1 AT2G19070 24.724 453 278 19 27 438 19 449 7.58e-20 92.0
MS.gene50356.t1 AT5G63560 25.978 358 219 13 50 393 56 381 1.50e-19 90.9
MS.gene50356.t1 AT5G39080 26.464 461 258 23 14 422 14 445 7.76e-18 85.9
MS.gene50356.t1 AT3G29635 24.365 394 263 12 21 392 26 406 1.69e-16 82.0
MS.gene50356.t1 AT1G03940 25.322 466 278 18 5 425 9 449 2.87e-16 81.3
MS.gene50356.t1 AT1G28680 23.573 403 255 16 15 395 18 389 6.87e-16 80.1
MS.gene50356.t1 AT5G61160 25.455 275 179 10 135 393 139 403 6.90e-16 80.1
MS.gene50356.t1 AT1G32910 26.575 365 216 15 53 393 63 399 2.52e-15 78.2
MS.gene50356.t1 AT1G24430 25.000 388 242 16 41 414 45 397 5.42e-15 77.0
MS.gene50356.t1 AT1G27620 23.958 384 264 14 55 430 58 421 5.89e-15 77.0
MS.gene50356.t1 AT5G39090 26.812 276 168 10 135 392 140 399 8.94e-15 76.6
MS.gene50356.t1 AT5G16410 24.802 379 243 15 41 399 77 433 1.43e-14 75.9
MS.gene50356.t1 AT1G28680 24.000 350 230 13 15 349 18 346 2.56e-14 74.7
MS.gene50356.t1 AT1G03390 25.341 367 225 16 48 393 69 407 2.02e-13 72.4
MS.gene50356.t1 AT5G23970 25.000 440 271 18 4 423 3 403 2.37e-13 72.0
MS.gene50356.t1 AT3G26040 23.133 415 257 14 50 441 54 429 4.45e-13 71.2
MS.gene50356.t1 AT3G30280 21.813 353 236 9 54 393 61 386 5.44e-13 70.9
MS.gene50356.t1 AT5G47980 24.570 407 250 18 54 437 60 432 8.13e-13 70.5
MS.gene50356.t1 AT5G39050 25.338 296 179 12 149 415 160 442 8.59e-13 70.5
MS.gene50356.t1 AT1G78990 25.627 359 222 15 53 393 65 396 1.33e-12 69.7
MS.gene50356.t1 AT5G07080 25.758 330 195 14 17 322 28 331 2.24e-12 68.9
MS.gene50356.t1 AT2G25150 23.967 363 239 15 50 396 62 403 1.07e-11 67.0
MS.gene50356.t1 AT5G17540 22.685 432 282 18 25 428 23 430 1.66e-11 66.2

Find 90 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCAAGGTTTGATGTTTA+TGG 0.150931 4.4:+78611987 MS.gene50356:CDS
CCATTACCAGAAGATTATTT+TGG 0.185967 4.4:+78611756 MS.gene50356:CDS
ACTTCTTTCAACCAACAAAA+AGG 0.205483 4.4:+78610954 MS.gene50356:CDS
AATGGATAGGAAATTATTAT+TGG 0.237638 4.4:-78611958 None:intergenic
TTCAATCATGTTGTTGTTGA+TGG 0.263269 4.4:+78611324 MS.gene50356:CDS
ATAGTTTGATCACTTGATTT+TGG 0.265670 4.4:-78610901 None:intergenic
CTAGTTGACGGTATCTTTAT+TGG 0.271020 4.4:+78611294 MS.gene50356:CDS
CTTAGTGAAAGGAAATATTA+TGG 0.298942 4.4:-78611463 None:intergenic
ATGTCTTCCTTACGAGGTTT+TGG 0.299283 4.4:+78612118 MS.gene50356:CDS
GATGTTTATGGTAATGATTT+TGG 0.308658 4.4:+78611999 MS.gene50356:CDS
GGAATTGTTAATGTATTTGC+TGG 0.313464 4.4:+78612065 MS.gene50356:CDS
TTCTCGTCGGTCAAACTGTT+TGG 0.325468 4.4:-78611685 None:intergenic
AGTGGCTCAGCCGTTGGATT+TGG 0.349065 4.4:-78611008 None:intergenic
CCAAAATAATCTTCTGGTAA+TGG 0.369361 4.4:-78611756 None:intergenic
TGAAGAATCACTATGAATCT+TGG 0.374462 4.4:+78611898 MS.gene50356:CDS
TGGATTTGGTCAGCTTCAAC+TGG 0.382433 4.4:-78610994 None:intergenic
GAGTTTCCAAAATAATCTTC+TGG 0.384510 4.4:-78611762 None:intergenic
ATGGGCCGTTGAACACCATT+TGG 0.389138 4.4:-78611444 None:intergenic
AAACCTTGGAGAACTACTAA+TGG 0.391518 4.4:-78611976 None:intergenic
TGGTTGAAAGAAGTAGAAAC+TGG 0.396348 4.4:-78610945 None:intergenic
GGGAAGTGACCCGCATTTCA+TGG 0.402104 4.4:+78612148 MS.gene50356:CDS
GCCGGCAAGAGGTTGGAAAA+AGG 0.411903 4.4:-78611049 None:intergenic
CGTAGTGGATATAAAATAAA+CGG 0.412390 4.4:+78612044 MS.gene50356:CDS
TATCCACTACGAACTGCTAT+AGG 0.429557 4.4:-78612032 None:intergenic
TCGAAAATCAATGAAGAGAT+TGG 0.431338 4.4:+78611600 MS.gene50356:CDS
CATAATATTTCCTTTCACTA+AGG 0.435233 4.4:+78611464 MS.gene50356:CDS
TGTATAGTCAAAATGAAAGT+TGG 0.440205 4.4:+78611795 MS.gene50356:CDS
AAGCGCTATTAACACACCTT+TGG 0.445268 4.4:+78611646 MS.gene50356:CDS
AGGCACATCAAAACCTTTGT+TGG 0.448515 4.4:+78611254 MS.gene50356:CDS
AATGAAAGTTGGTGAGTTGT+TGG 0.448624 4.4:+78611806 MS.gene50356:CDS
TTAGTGAAAGGAAATATTAT+GGG 0.456793 4.4:-78611462 None:intergenic
ACCTGCATTGTTGCATATAA+CGG 0.463614 4.4:-78611115 None:intergenic
GTATATAATCTAATCGTGAT+AGG 0.468498 4.4:+78611711 MS.gene50356:CDS
GAGGTACGTAGACTGGTTCT+AGG 0.471874 4.4:-78611185 None:intergenic
CTAGCAACCCTAACGAAGCT+TGG 0.478486 4.4:-78611930 None:intergenic
AATCTAATCGTGATAGGAGT+TGG 0.478549 4.4:+78611717 MS.gene50356:CDS
TGTGGCATTTCATCAATTCA+TGG 0.479156 4.4:+78611355 MS.gene50356:CDS
GACGAAGGTGGACTATATAA+AGG 0.481976 4.4:+78611828 MS.gene50356:CDS
TTCCGTTATTTCAAGACGGC+CGG 0.498398 4.4:-78611067 None:intergenic
AAACTATTGATTTGACTCCA+TGG 0.507614 4.4:+78610917 MS.gene50356:CDS
AACCATGAAGCTGCATTAGT+TGG 0.510186 4.4:-78612180 None:intergenic
TGCCGGCCGTCTTGAAATAA+CGG 0.512312 4.4:+78611065 MS.gene50356:CDS
CTAGAGCGTTGGTTTCCAAA+TGG 0.513520 4.4:+78611429 MS.gene50356:CDS
AACTATTGATTTGACTCCAT+GGG 0.516810 4.4:+78610918 MS.gene50356:CDS
GGTTTCCAAATGGTGTTCAA+CGG 0.518919 4.4:+78611439 MS.gene50356:CDS
CGTAAGGAAGACATACTTGA+AGG 0.521339 4.4:-78612109 None:intergenic
CCACAAAAGAATCACTCTGA+TGG 0.525279 4.4:+78611489 MS.gene50356:CDS
GTGGCATTTCATCAATTCAT+GGG 0.527709 4.4:+78611356 MS.gene50356:CDS
AACCAACGCTCTAGTGTTGG+AGG 0.532701 4.4:-78611420 None:intergenic
CAAGACGGCCGGCAAGAGGT+TGG 0.533473 4.4:-78611056 None:intergenic
ACAGAGAGTGGCTCAGCCGT+TGG 0.535281 4.4:-78611014 None:intergenic
ACAATTGGAGGTACGTAGAC+TGG 0.544025 4.4:-78611192 None:intergenic
AAATAATCTTCTGGTAATGG+TGG 0.545669 4.4:-78611753 None:intergenic
CACAAAAGAATCACTCTGAT+GGG 0.546152 4.4:+78611490 MS.gene50356:CDS
ATTTCAAGACGGCCGGCAAG+AGG 0.547420 4.4:-78611060 None:intergenic
GATTATATACTTCTTCTCGT+CGG 0.547635 4.4:-78611698 None:intergenic
AACCTCCAACACTAGAGCGT+TGG 0.548790 4.4:+78611418 MS.gene50356:CDS
TCCGTTATATGCAACAATGC+AGG 0.551070 4.4:+78611114 MS.gene50356:CDS
TTGGAGAACTACTAATGGAT+AGG 0.555964 4.4:-78611971 None:intergenic
TTTGTTGGCTGTTCAAGTAA+CGG 0.556867 4.4:+78611269 MS.gene50356:CDS
ATTGCCTCCAAAACCTCGTA+AGG 0.558716 4.4:-78612125 None:intergenic
GGAAACCAACGCTCTAGTGT+TGG 0.568216 4.4:-78611423 None:intergenic
TCACGACATCCATGAAATGC+GGG 0.574220 4.4:-78612157 None:intergenic
GGTAATGGTGGAACAAACCT+TGG 0.575179 4.4:-78611741 None:intergenic
GTATTTGCTGGAATAGAAGA+AGG 0.577275 4.4:+78612077 MS.gene50356:CDS
TCTCTCCACTGATACAATCA+AGG 0.580251 4.4:+78610876 MS.gene50356:CDS
CAAGAGCAGAGGACAGAGAG+TGG 0.582669 4.4:-78611026 None:intergenic
TTACCATAAACATCAAACCT+TGG 0.582755 4.4:-78611990 None:intergenic
TCAAGTATGTCTTCCTTACG+AGG 0.584114 4.4:+78612112 MS.gene50356:CDS
CAAGTAACGGAGCTAGTTGA+CGG 0.586570 4.4:+78611282 MS.gene50356:CDS
TTTGGTCAGCTTCAACTGGA+TGG 0.587605 4.4:-78610990 None:intergenic
TGGTGCCTTGATTGTATCAG+TGG 0.587630 4.4:-78610881 None:intergenic
TGTGTTCCGTTATTTCAAGA+CGG 0.588005 4.4:-78611071 None:intergenic
GTTGGTGAGTTGTTGGACGA+AGG 0.590545 4.4:+78611813 MS.gene50356:CDS
CAAGTTAATCGCTTCACACA+CGG 0.591631 4.4:+78611866 MS.gene50356:CDS
AGTAGAAACTGGAGATCCCA+TGG 0.592204 4.4:-78610934 None:intergenic
AACGGATGACAGAGCACCAA+AGG 0.598048 4.4:-78611662 None:intergenic
TATCCATTAGTAGTTCTCCA+AGG 0.603764 4.4:+78611973 MS.gene50356:CDS
GTCGGTCAAACTGTTTGGAA+CGG 0.617663 4.4:-78611680 None:intergenic
ATCAGTGGAGAGAACTCGTA+CGG 0.619099 4.4:-78610866 None:intergenic
GTCACGACATCCATGAAATG+CGG 0.620554 4.4:-78612158 None:intergenic
GTTACTTGAACAGCCAACAA+AGG 0.636862 4.4:-78611267 None:intergenic
GGTGAGTTGTTGGACGAAGG+TGG 0.644953 4.4:+78611816 MS.gene50356:CDS
TCGTGATAGGAGTTGGACCA+AGG 0.646491 4.4:+78611724 MS.gene50356:CDS
ACACCTATAGCAGTTCGTAG+TGG 0.662092 4.4:+78612029 MS.gene50356:CDS
TAGGTTATTCCACTTCACAA+AGG 0.669253 4.4:+78611557 MS.gene50356:CDS
TTGAAGCTGACCAAATCCAA+CGG 0.669951 4.4:+78610998 MS.gene50356:CDS
TGAATTGATGAAATGCCACA+CGG 0.683993 4.4:-78611352 None:intergenic
AAGTATTCTCAGCTGCAGCG+TGG 0.695338 4.4:-78611149 None:intergenic
TTGTTGATGGAAAATCCGTG+TGG 0.770650 4.4:+78611337 MS.gene50356:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGGATAGGAAATTATTAT+TGG - chr4.4:78611961-78611980 None:intergenic 20.0%
!! TTAGTGAAAGGAAATATTAT+GGG - chr4.4:78611465-78611484 None:intergenic 20.0%
!!! ATAGTTTTTATCTCCAATAA+AGG - chr4.4:78611232-78611251 None:intergenic 20.0%
! AAAAACTCTGCTTAACAAAT+AGG + chr4.4:78611538-78611557 MS.gene50356:CDS 25.0%
! ATTGGAGATAAAAACTATGA+AGG + chr4.4:78611234-78611253 MS.gene50356:CDS 25.0%
! CATAATATTTCCTTTCACTA+AGG + chr4.4:78611464-78611483 MS.gene50356:CDS 25.0%
! CTTAGTGAAAGGAAATATTA+TGG - chr4.4:78611466-78611485 None:intergenic 25.0%
! GTATATAATCTAATCGTGAT+AGG + chr4.4:78611711-78611730 MS.gene50356:CDS 25.0%
! TGTATAGTCAAAATGAAAGT+TGG + chr4.4:78611795-78611814 MS.gene50356:CDS 25.0%
!! ATAGTTTGATCACTTGATTT+TGG - chr4.4:78610904-78610923 None:intergenic 25.0%
!!! CGTAGTGGATATAAAATAAA+CGG + chr4.4:78612044-78612063 MS.gene50356:CDS 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr4.4:78611999-78612018 MS.gene50356:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG + chr4.4:78612004-78612023 MS.gene50356:CDS 25.0%
!!! TTGTAGAGAAGATATTTTGA+TGG - chr4.4:78611628-78611647 None:intergenic 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG + chr4.4:78612003-78612022 MS.gene50356:CDS 25.0%
AAAAAATCCAAGCTTCGTTA+GGG + chr4.4:78611923-78611942 MS.gene50356:CDS 30.0%
AAAAATGAATCGACAATTGG+AGG - chr4.4:78611207-78611226 None:intergenic 30.0%
ACTTCTTTCAACCAACAAAA+AGG + chr4.4:78610954-78610973 MS.gene50356:CDS 30.0%
CCAAAATAATCTTCTGGTAA+TGG - chr4.4:78611759-78611778 None:intergenic 30.0%
CTTCTTTCAACCAACAAAAA+GGG + chr4.4:78610955-78610974 MS.gene50356:CDS 30.0%
GAGTTTCCAAAATAATCTTC+TGG - chr4.4:78611765-78611784 None:intergenic 30.0%
GATTATATACTTCTTCTCGT+CGG - chr4.4:78611701-78611720 None:intergenic 30.0%
GGAATTGTTAATGTATTTGC+TGG + chr4.4:78612065-78612084 MS.gene50356:CDS 30.0%
TAAAAAATCCAAGCTTCGTT+AGG + chr4.4:78611922-78611941 MS.gene50356:CDS 30.0%
TCGAAAATCAATGAAGAGAT+TGG + chr4.4:78611600-78611619 MS.gene50356:CDS 30.0%
TGAAGAATCACTATGAATCT+TGG + chr4.4:78611898-78611917 MS.gene50356:CDS 30.0%
TTACCATAAACATCAAACCT+TGG - chr4.4:78611993-78612012 None:intergenic 30.0%
! ATAAAGAAGACCCTTTTTGT+TGG - chr4.4:78610968-78610987 None:intergenic 30.0%
! CCATTACCAGAAGATTATTT+TGG + chr4.4:78611756-78611775 MS.gene50356:CDS 30.0%
! TAATTTTCTCCTTTGTGAAG+TGG - chr4.4:78611569-78611588 None:intergenic 30.0%
! TTCAATCATGTTGTTGTTGA+TGG + chr4.4:78611324-78611343 MS.gene50356:CDS 30.0%
!! AAACTATTGATTTGACTCCA+TGG + chr4.4:78610917-78610936 MS.gene50356:CDS 30.0%
!! AAATAATCTTCTGGTAATGG+TGG - chr4.4:78611756-78611775 None:intergenic 30.0%
!! AACTATTGATTTGACTCCAT+GGG + chr4.4:78610918-78610937 MS.gene50356:CDS 30.0%
!! GATTCATTTTTCGCCTTTAT+TGG + chr4.4:78611216-78611235 MS.gene50356:CDS 30.0%
!! TATGGTAATGATTTTGGATG+GGG + chr4.4:78612005-78612024 MS.gene50356:CDS 30.0%
AAACCTTGGAGAACTACTAA+TGG - chr4.4:78611979-78611998 None:intergenic 35.0%
AATCTAATCGTGATAGGAGT+TGG + chr4.4:78611717-78611736 MS.gene50356:CDS 35.0%
ACCTGCATTGTTGCATATAA+CGG - chr4.4:78611118-78611137 None:intergenic 35.0%
CACAAAAGAATCACTCTGAT+GGG + chr4.4:78611490-78611509 MS.gene50356:CDS 35.0%
CTAGTTGACGGTATCTTTAT+TGG + chr4.4:78611294-78611313 MS.gene50356:CDS 35.0%
GCGAAAAATGAATCGACAAT+TGG - chr4.4:78611210-78611229 None:intergenic 35.0%
GTGGCATTTCATCAATTCAT+GGG + chr4.4:78611356-78611375 MS.gene50356:CDS 35.0%
TAGGTTATTCCACTTCACAA+AGG + chr4.4:78611557-78611576 MS.gene50356:CDS 35.0%
TATCCATTAGTAGTTCTCCA+AGG + chr4.4:78611973-78611992 MS.gene50356:CDS 35.0%
TCTCCAAGGTTTGATGTTTA+TGG + chr4.4:78611987-78612006 MS.gene50356:CDS 35.0%
TGAATTGATGAAATGCCACA+CGG - chr4.4:78611355-78611374 None:intergenic 35.0%
TGGTTGAAAGAAGTAGAAAC+TGG - chr4.4:78610948-78610967 None:intergenic 35.0%
TGTGGCATTTCATCAATTCA+TGG + chr4.4:78611355-78611374 MS.gene50356:CDS 35.0%
! GTATTTGCTGGAATAGAAGA+AGG + chr4.4:78612077-78612096 MS.gene50356:CDS 35.0%
! TGTGTTCCGTTATTTCAAGA+CGG - chr4.4:78611074-78611093 None:intergenic 35.0%
! TTGGAGAACTACTAATGGAT+AGG - chr4.4:78611974-78611993 None:intergenic 35.0%
! TTTGTTGGCTGTTCAAGTAA+CGG + chr4.4:78611269-78611288 MS.gene50356:CDS 35.0%
!! AATGAAAGTTGGTGAGTTGT+TGG + chr4.4:78611806-78611825 MS.gene50356:CDS 35.0%
AAGCGCTATTAACACACCTT+TGG + chr4.4:78611646-78611665 MS.gene50356:CDS 40.0%
AGGCACATCAAAACCTTTGT+TGG + chr4.4:78611254-78611273 MS.gene50356:CDS 40.0%
CAAGTTAATCGCTTCACACA+CGG + chr4.4:78611866-78611885 MS.gene50356:CDS 40.0%
CCACAAAAGAATCACTCTGA+TGG + chr4.4:78611489-78611508 MS.gene50356:CDS 40.0%
CGTAAGGAAGACATACTTGA+AGG - chr4.4:78612112-78612131 None:intergenic 40.0%
GGTTTCCAAATGGTGTTCAA+CGG + chr4.4:78611439-78611458 MS.gene50356:CDS 40.0%
GTTACTTGAACAGCCAACAA+AGG - chr4.4:78611270-78611289 None:intergenic 40.0%
TATCCACTACGAACTGCTAT+AGG - chr4.4:78612035-78612054 None:intergenic 40.0%
TCAAGTATGTCTTCCTTACG+AGG + chr4.4:78612112-78612131 MS.gene50356:CDS 40.0%
TCCGTTATATGCAACAATGC+AGG + chr4.4:78611114-78611133 MS.gene50356:CDS 40.0%
TCTCTCCACTGATACAATCA+AGG + chr4.4:78610876-78610895 MS.gene50356:CDS 40.0%
TTGAAGCTGACCAAATCCAA+CGG + chr4.4:78610998-78611017 MS.gene50356:CDS 40.0%
! ATGTCTTCCTTACGAGGTTT+TGG + chr4.4:78612118-78612137 MS.gene50356:CDS 40.0%
! CCATCAGAGTGATTCTTTTG+TGG - chr4.4:78611492-78611511 None:intergenic 40.0%
! TTGTTGATGGAAAATCCGTG+TGG + chr4.4:78611337-78611356 MS.gene50356:CDS 40.0%
! TTTTGTGGCTCCTTAGTGAA+AGG - chr4.4:78611477-78611496 None:intergenic 40.0%
!! GACGAAGGTGGACTATATAA+AGG + chr4.4:78611828-78611847 MS.gene50356:CDS 40.0%
ACACCTATAGCAGTTCGTAG+TGG + chr4.4:78612029-78612048 MS.gene50356:CDS 45.0%
AGTAGAAACTGGAGATCCCA+TGG - chr4.4:78610937-78610956 None:intergenic 45.0%
ATCAGTGGAGAGAACTCGTA+CGG - chr4.4:78610869-78610888 None:intergenic 45.0%
ATTGCCTCCAAAACCTCGTA+AGG - chr4.4:78612128-78612147 None:intergenic 45.0%
GTCACGACATCCATGAAATG+CGG - chr4.4:78612161-78612180 None:intergenic 45.0%
GTCGGTCAAACTGTTTGGAA+CGG - chr4.4:78611683-78611702 None:intergenic 45.0%
TCACGACATCCATGAAATGC+GGG - chr4.4:78612160-78612179 None:intergenic 45.0%
TGGTGCCTTGATTGTATCAG+TGG - chr4.4:78610884-78610903 None:intergenic 45.0%
TTCTCGTCGGTCAAACTGTT+TGG - chr4.4:78611688-78611707 None:intergenic 45.0%
TTGGAAAAAGGCAAGAGCAG+AGG - chr4.4:78611040-78611059 None:intergenic 45.0%
TTTGGTCAGCTTCAACTGGA+TGG - chr4.4:78610993-78611012 None:intergenic 45.0%
! ACAATTGGAGGTACGTAGAC+TGG - chr4.4:78611195-78611214 None:intergenic 45.0%
! CAAGTAACGGAGCTAGTTGA+CGG + chr4.4:78611282-78611301 MS.gene50356:CDS 45.0%
! GGTAATGGTGGAACAAACCT+TGG - chr4.4:78611744-78611763 None:intergenic 45.0%
! TGGATTTGGTCAGCTTCAAC+TGG - chr4.4:78610997-78611016 None:intergenic 45.0%
! TTCCGTTATTTCAAGACGGC+CGG - chr4.4:78611070-78611089 None:intergenic 45.0%
!! CTAGAGCGTTGGTTTCCAAA+TGG + chr4.4:78611429-78611448 MS.gene50356:CDS 45.0%
!! TCTTCCTTACGAGGTTTTGG+AGG + chr4.4:78612121-78612140 MS.gene50356:CDS 45.0%
!!! TACGAGGTTTTGGAGGCAAT+GGG + chr4.4:78612128-78612147 MS.gene50356:CDS 45.0%
!!! TTACGAGGTTTTGGAGGCAA+TGG + chr4.4:78612127-78612146 MS.gene50356:CDS 45.0%
AACCTCCAACACTAGAGCGT+TGG + chr4.4:78611418-78611437 MS.gene50356:CDS 50.0%
AAGTATTCTCAGCTGCAGCG+TGG - chr4.4:78611152-78611171 None:intergenic 50.0%
ATGGGCCGTTGAACACCATT+TGG - chr4.4:78611447-78611466 None:intergenic 50.0%
CTAGCAACCCTAACGAAGCT+TGG - chr4.4:78611933-78611952 None:intergenic 50.0%
GGAAACCAACGCTCTAGTGT+TGG - chr4.4:78611426-78611445 None:intergenic 50.0%
TCGTGATAGGAGTTGGACCA+AGG + chr4.4:78611724-78611743 MS.gene50356:CDS 50.0%
TGCCGGCCGTCTTGAAATAA+CGG + chr4.4:78611065-78611084 MS.gene50356:CDS 50.0%
! AACCAACGCTCTAGTGTTGG+AGG - chr4.4:78611423-78611442 None:intergenic 50.0%
! AACGGATGACAGAGCACCAA+AGG - chr4.4:78611665-78611684 None:intergenic 50.0%
! GAGGTACGTAGACTGGTTCT+AGG - chr4.4:78611188-78611207 None:intergenic 50.0%
! GCCTTTTTCCAACCTCTTGC+CGG + chr4.4:78611048-78611067 MS.gene50356:CDS 50.0%
! GTTGGTGAGTTGTTGGACGA+AGG + chr4.4:78611813-78611832 MS.gene50356:CDS 50.0%
AGTGGCTCAGCCGTTGGATT+TGG - chr4.4:78611011-78611030 None:intergenic 55.0%
ATTTCAAGACGGCCGGCAAG+AGG - chr4.4:78611063-78611082 None:intergenic 55.0%
CAAGAGCAGAGGACAGAGAG+TGG - chr4.4:78611029-78611048 None:intergenic 55.0%
GCCGGCAAGAGGTTGGAAAA+AGG - chr4.4:78611052-78611071 None:intergenic 55.0%
GGGAAGTGACCCGCATTTCA+TGG + chr4.4:78612148-78612167 MS.gene50356:CDS 55.0%
GGTGAGTTGTTGGACGAAGG+TGG + chr4.4:78611816-78611835 MS.gene50356:CDS 55.0%
ACAGAGAGTGGCTCAGCCGT+TGG - chr4.4:78611017-78611036 None:intergenic 60.0%
CAAGACGGCCGGCAAGAGGT+TGG - chr4.4:78611059-78611078 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 78610859 78612187 78610859 ID=MS.gene50356
chr4.4 mRNA 78610859 78612187 78610859 ID=MS.gene50356.t1;Parent=MS.gene50356
chr4.4 exon 78610859 78612187 78610859 ID=MS.gene50356.t1.exon1;Parent=MS.gene50356.t1
chr4.4 CDS 78610859 78612187 78610859 ID=cds.MS.gene50356.t1;Parent=MS.gene50356.t1
Gene Sequence

>MS.gene50356

ATGGGAACCGTACGAGTTCTCTCCACTGATACAATCAAGGCACCAAAATCAAGTGATCAAACTATTGATTTGACTCCATGGGATCTCCAGTTTCTACTTCTTTCAACCAACAAAAAGGGTCTTCTTTATCACCATCCAGTTGAAGCTGACCAAATCCAACGGCTGAGCCACTCTCTGTCCTCTGCTCTTGCCTTTTTCCAACCTCTTGCCGGCCGTCTTGAAATAACGGAACACAGAGACAACATTGTCTCGTGTTCCGTTATATGCAACAATGCAGGTGTACTCTTTATCCACGCTGCAGCTGAGAATACTTGTGTTGCTGACATCCTAGAACCAGTCTACGTACCTCCAATTGTCGATTCATTTTTCGCCTTTATTGGAGATAAAAACTATGAAGGCACATCAAAACCTTTGTTGGCTGTTCAAGTAACGGAGCTAGTTGACGGTATCTTTATTGGTTGTACATTCAATCATGTTGTTGTTGATGGAAAATCCGTGTGGCATTTCATCAATTCATGGGCTGAAATCTCACGTAGTTGTTGTCATGATCAAATATCTAAACCTCCAACACTAGAGCGTTGGTTTCCAAATGGTGTTCAACGGCCCATAATATTTCCTTTCACTAAGGAGCCACAAAAGAATCACTCTGATGGGCTCAGTTTTTCGTCATTAAATGACGAAAAACTCTGCTTAACAAATAGGTTATTCCACTTCACAAAGGAGAAAATTATGCAGCTGAAATCGAAAATCAATGAAGAGATTGGTACCATCAAAATATCTTCTCTACAAGCGCTATTAACACACCTTTGGTGCTCTGTCATCCGTTCCAAACAGTTTGACCGACGAGAAGAAGTATATAATCTAATCGTGATAGGAGTTGGACCAAGGTTTGTTCCACCATTACCAGAAGATTATTTTGGAAACTCGATTATAAGTTGTATAGTCAAAATGAAAGTTGGTGAGTTGTTGGACGAAGGTGGACTATATAAAGGTGCGTGTGAGATGAACAAGTTAATCGCTTCACACACGGATGAGAAGTTGAAGAATCACTATGAATCTTGGTTAAAAAATCCAAGCTTCGTTAGGGTTGCTAGCATGTCCAATAATAATTTCCTATCCATTAGTAGTTCTCCAAGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAACACCTATAGCAGTTCGTAGTGGATATAAAATAAACGGAATTGTTAATGTATTTGCTGGAATAGAAGAAGGTAGTATTGACCTTCAAGTATGTCTTCCTTACGAGGTTTTGGAGGCAATGGGAAGTGACCCGCATTTCATGGATGTCGTGACCAACTAA
Protein sequence

>MS.gene50356.t1

MGTVRVLSTDTIKAPKSSDQTIDLTPWDLQFLLLSTNKKGLLYHHPVEADQIQRLSHSLSSALAFFQPLAGRLEITEHRDNIVSCSVICNNAGVLFIHAAAENTCVADILEPVYVPPIVDSFFAFIGDKNYEGTSKPLLAVQVTELVDGIFIGCTFNHVVVDGKSVWHFINSWAEISRSCCHDQISKPPTLERWFPNGVQRPIIFPFTKEPQKNHSDGLSFSSLNDEKLCLTNRLFHFTKEKIMQLKSKINEEIGTIKISSLQALLTHLWCSVIRSKQFDRREEVYNLIVIGVGPRFVPPLPEDYFGNSIISCIVKMKVGELLDEGGLYKGACEMNKLIASHTDEKLKNHYESWLKNPSFVRVASMSNNNFLSISSSPRFDVYGNDFGWGTPIAVRSGYKINGIVNVFAGIEEGSIDLQVCLPYEVLEAMGSDPHFMDVVTN