AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90493

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90493.t1 MTR_4g094825 63.848 343 88 8 18 355 80 391 5.56e-137 395
MS.gene90493.t1 MTR_4g094838 45.370 432 156 9 4 355 1 432 1.07e-105 317
MS.gene90493.t1 MTR_4g094835 68.750 208 63 1 151 356 240 447 1.03e-95 292
MS.gene90493.t1 MTR_4g094835 69.677 155 42 4 1 151 1 154 1.61e-63 209
MS.gene90493.t1 MTR_4g094832 68.599 207 63 1 151 355 224 430 2.05e-95 291
MS.gene90493.t1 MTR_4g094832 56.471 85 24 2 77 151 57 138 7.79e-21 93.6
MS.gene90493.t1 MTR_0190s0020 59.709 206 79 3 151 355 240 442 7.18e-78 246
MS.gene90493.t1 MTR_0190s0020 54.839 155 62 4 1 151 1 151 2.94e-43 155
MS.gene90493.t1 MTR_4g094842 59.406 202 78 3 151 351 246 444 8.00e-78 246
MS.gene90493.t1 MTR_4g094842 55.346 159 60 3 1 151 1 156 2.26e-49 172
MS.gene90493.t1 MTR_4g022825 59.113 203 78 3 151 352 239 437 1.09e-75 240
MS.gene90493.t1 MTR_4g022825 49.669 151 67 4 1 151 1 142 1.04e-37 140
MS.gene90493.t1 MTR_4g022800 58.937 207 79 4 151 355 240 442 1.23e-73 235
MS.gene90493.t1 MTR_4g022800 49.669 151 67 4 1 151 1 142 8.57e-38 141
MS.gene90493.t1 MTR_8g041770 55.825 206 87 3 151 355 239 441 1.98e-73 234
MS.gene90493.t1 MTR_8g041770 52.258 155 66 4 1 151 1 151 1.96e-42 153
MS.gene90493.t1 MTR_4g022720 57.767 206 82 3 151 355 234 435 1.76e-72 232
MS.gene90493.t1 MTR_4g022720 50.331 151 66 4 1 151 1 142 9.00e-39 143
MS.gene90493.t1 MTR_3g088420 57.282 206 83 3 151 355 232 433 3.82e-72 231
MS.gene90493.t1 MTR_3g088420 58.940 151 53 5 1 151 1 142 6.28e-48 168
MS.gene90493.t1 MTR_4g022670 55.665 203 85 3 151 352 240 438 3.09e-71 229
MS.gene90493.t1 MTR_4g022670 49.682 157 68 6 1 157 1 146 1.17e-35 135
MS.gene90493.t1 MTR_7g026860 57.282 206 80 4 151 355 240 438 2.42e-69 224
MS.gene90493.t1 MTR_7g026860 50.993 151 65 5 1 151 1 142 3.84e-37 139
MS.gene90493.t1 MTR_8g040780 54.187 203 88 3 151 350 27 227 4.07e-69 217
MS.gene90493.t1 MTR_3g026310 51.707 205 92 4 151 352 245 445 1.57e-63 209
MS.gene90493.t1 MTR_3g026310 56.291 151 57 5 1 151 1 142 4.76e-43 155
MS.gene90493.t1 MTR_4g094828 49.296 213 98 6 151 355 240 450 6.75e-63 207
MS.gene90493.t1 MTR_4g094828 61.438 153 51 3 1 151 1 147 6.09e-53 181
MS.gene90493.t1 MTR_8g079710 51.707 205 89 6 151 346 233 436 8.54e-60 199
MS.gene90493.t1 MTR_8g079710 61.438 153 51 3 1 151 1 147 2.01e-52 180
MS.gene90493.t1 MTR_6g016815 32.338 402 190 13 1 355 15 381 4.02e-57 191
MS.gene90493.t1 MTR_4g022290 60.927 151 56 1 1 151 1 148 2.61e-54 185
MS.gene90493.t1 MTR_4g022290 46.948 213 100 6 151 352 232 442 9.54e-52 178
MS.gene90493.t1 MTR_4g022760 46.117 206 62 3 151 355 240 397 3.35e-49 170
MS.gene90493.t1 MTR_4g022760 48.344 151 69 5 1 151 1 142 9.20e-35 132
MS.gene90493.t1 MTR_3g102020 40.488 205 119 2 152 355 249 451 3.62e-44 158
MS.gene90493.t1 MTR_3g102020 40.000 135 70 4 26 151 25 157 5.41e-17 82.4
MS.gene90493.t1 MTR_5g081960 39.474 190 113 2 163 351 294 482 3.63e-43 156
MS.gene90493.t1 MTR_5g081960 30.769 195 120 6 23 210 20 206 1.75e-12 68.6
MS.gene90493.t1 MTR_3g101900 39.024 205 122 2 152 355 246 448 1.09e-42 154
MS.gene90493.t1 MTR_3g101900 38.168 131 75 3 26 151 26 155 1.18e-15 78.2
MS.gene90493.t1 MTR_3g101930 39.303 201 120 1 152 352 254 452 3.68e-42 153
MS.gene90493.t1 MTR_3g101930 38.462 143 75 5 20 151 19 159 2.65e-16 80.1
MS.gene90493.t1 MTR_3g101970 39.604 202 119 2 152 352 235 434 4.77e-42 152
MS.gene90493.t1 MTR_3g101970 37.313 134 75 3 26 151 25 157 7.77e-15 75.9
MS.gene90493.t1 MTR_3g101960 39.130 207 123 2 152 357 248 452 6.15e-41 149
MS.gene90493.t1 MTR_3g101960 37.594 133 75 3 26 151 25 156 1.22e-15 78.2
MS.gene90493.t1 MTR_3g102010 39.024 205 122 2 152 355 249 451 8.96e-41 149
MS.gene90493.t1 MTR_3g102010 40.769 130 71 4 26 151 26 153 3.74e-17 82.8
MS.gene90493.t1 MTR_8g040760 51.316 152 69 4 1 151 1 148 6.94e-40 140
MS.gene90493.t1 MTR_3g070170 36.889 225 133 5 134 351 244 466 3.37e-36 137
MS.gene90493.t1 MTR_3g070170 36.434 129 77 2 23 151 26 149 8.03e-15 75.9
MS.gene90493.t1 MTR_4g015180 36.957 184 114 2 169 351 301 483 6.61e-35 134
MS.gene90493.t1 MTR_6g045553 33.498 203 132 3 151 351 270 471 3.00e-27 112
MS.gene90493.t1 MTR_7g026850 48.718 117 57 3 35 151 12 125 1.18e-26 104
MS.gene90493.t1 MTR_6g034485 59.036 83 32 1 151 233 45 125 3.28e-26 102
MS.gene90493.t1 MTR_7g026770 35.377 212 97 7 77 250 92 301 2.26e-24 101
MS.gene90493.t1 MTR_7g026780 43.750 112 37 2 244 355 26 111 1.65e-20 86.3
MS.gene90493.t1 MTR_6g034495 46.226 106 48 3 55 151 19 124 5.53e-20 86.3
MS.gene90493.t1 MTR_3g102030 32.812 128 86 0 182 309 193 320 4.09e-19 87.4
MS.gene90493.t1 MTR_3g098980 31.351 185 118 5 152 336 243 418 1.18e-16 81.3
MS.gene90493.t1 MTR_3g026950 27.317 205 131 5 152 340 235 437 1.20e-15 78.2
MS.gene90493.t1 MTR_7g026830 54.237 59 27 0 93 151 16 74 5.23e-15 70.5
MS.gene90493.t1 MTR_4g007540 29.353 201 133 7 151 346 232 428 5.70e-15 76.3
MS.gene90493.t1 MTR_3g049140 27.228 202 134 5 150 340 31 230 6.65e-15 73.9
MS.gene90493.t1 MTR_3g049150 26.368 201 134 5 152 340 235 433 1.85e-14 74.7
MS.gene90493.t1 MTR_8g075610 27.778 198 134 6 151 344 221 413 2.08e-14 74.3
MS.gene90493.t1 MTR_8g096530 33.766 154 95 4 151 304 236 382 1.10e-13 72.4
MS.gene90493.t1 MTR_6g015320 30.065 153 100 4 158 307 261 409 5.21e-13 70.1
MS.gene90493.t1 MTR_6g015315 29.032 155 100 5 158 307 258 407 1.01e-12 69.3
MS.gene90493.t1 MTR_7g026880 56.863 51 20 1 302 352 2 50 2.43e-12 61.6
MS.gene90493.t1 MTR_7g026870 43.662 71 34 2 199 263 81 151 3.05e-12 64.3
MS.gene90493.t1 MTR_4g127650 23.558 208 145 3 151 344 241 448 3.40e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90493.t1 AT5G67150 41.919 198 113 1 154 351 247 442 4.88e-50 174
MS.gene90493.t1 AT5G67150 37.580 157 91 4 4 158 5 156 2.59e-22 98.2
MS.gene90493.t1 AT3G50280 36.893 206 128 1 151 354 107 312 7.89e-44 154
MS.gene90493.t1 AT3G50270 37.255 204 126 2 151 352 242 445 2.73e-43 156
MS.gene90493.t1 AT3G50270 36.364 154 93 3 6 158 7 156 2.87e-19 89.0
MS.gene90493.t1 AT3G50280 36.893 206 128 1 151 354 237 442 8.59e-43 154
MS.gene90493.t1 AT3G50280 35.526 152 93 3 1 151 1 148 4.62e-21 94.4
MS.gene90493.t1 AT5G01210 38.220 191 116 2 162 351 276 465 3.08e-41 150
MS.gene90493.t1 AT3G50300 36.245 229 138 3 136 356 220 448 3.58e-41 150
MS.gene90493.t1 AT3G50300 39.375 160 89 5 1 158 1 154 3.65e-22 97.4
MS.gene90493.t1 AT5G07860 36.515 241 135 6 125 351 209 445 1.20e-38 143
MS.gene90493.t1 AT5G07870 35.628 247 143 5 125 357 219 463 2.15e-38 143
MS.gene90493.t1 AT2G39980 35.961 203 128 2 150 351 275 476 6.96e-38 142
MS.gene90493.t1 AT2G39980 37.097 124 73 1 28 151 27 145 7.77e-17 82.0
MS.gene90493.t1 AT5G42830 35.821 201 126 2 152 351 247 445 8.42e-37 138
MS.gene90493.t1 AT5G42830 37.500 128 77 3 26 151 26 152 6.01e-17 82.4
MS.gene90493.t1 AT5G67160 38.119 202 114 4 151 350 235 427 1.86e-36 137
MS.gene90493.t1 AT5G67160 39.552 134 71 4 26 158 25 149 1.04e-19 90.5
MS.gene90493.t1 AT5G38130 35.450 189 120 1 151 337 255 443 2.89e-34 132
MS.gene90493.t1 AT5G38130 40.625 160 87 5 1 158 21 174 5.22e-23 100
MS.gene90493.t1 AT5G07850 36.364 209 129 3 151 357 249 455 1.86e-33 129
MS.gene90493.t1 AT5G07850 33.333 135 80 4 30 157 31 162 2.72e-12 68.2
MS.gene90493.t1 AT5G23940 32.174 230 142 5 135 351 220 448 1.73e-30 121
MS.gene90493.t1 AT3G50290 36.774 155 96 1 150 302 115 269 2.52e-28 112
MS.gene90493.t1 AT1G28680 28.992 238 131 10 97 307 163 389 2.60e-14 74.3
MS.gene90493.t1 AT2G19070 25.980 204 138 4 153 344 241 443 3.55e-14 73.9
MS.gene90493.t1 AT5G48930 27.919 197 133 6 151 342 232 424 3.09e-13 70.9
MS.gene90493.t1 AT3G29590 24.873 197 147 1 151 346 246 442 4.38e-13 70.5
MS.gene90493.t1 AT5G41040 30.270 185 117 6 152 332 246 422 2.08e-12 68.6
MS.gene90493.t1 AT5G41040 30.270 185 117 6 152 332 262 438 2.12e-12 68.6
MS.gene90493.t1 AT1G28680 33.636 110 70 1 152 261 240 346 2.13e-12 68.2
MS.gene90493.t1 AT2G40230 26.923 182 127 2 134 315 222 397 4.02e-12 67.4
MS.gene90493.t1 AT3G48720 27.778 198 129 6 152 344 239 427 1.56e-11 65.9

Find 87 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCTTTCTTATGAGATTT+TGG 0.242506 8.3:+23602741 MS.gene90493:CDS
TGTTCCACTTCCAAGTTCTA+TGG 0.245627 8.3:+23602799 MS.gene90493:CDS
TTCCGAAATAATCCTCTTCT+AGG 0.269532 8.3:-23602378 None:intergenic
GGGATTGGTAACAAGTATAA+TGG 0.290220 8.3:+23602667 MS.gene90493:CDS
TTGGTATTGGAATGGGTTGT+TGG 0.293902 8.3:-23601616 None:intergenic
GTTTCTTATGTGTTGTTTAT+AGG 0.297168 8.3:+23602331 MS.gene90493:CDS
GCAGGAGAGGTTCTAGAGTA+TGG 0.316001 8.3:+23602433 MS.gene90493:CDS
GGGGAATGACACTGAGTTCA+TGG 0.325881 8.3:+23602771 MS.gene90493:CDS
TACATTCTGATAAGATAATA+AGG 0.327777 8.3:+23602497 MS.gene90493:CDS
TTTGTTTAAGATGGTTTATA+TGG 0.335226 8.3:-23601640 None:intergenic
GAAACATCTTCTTGTGGATC+AGG 0.367065 8.3:-23602313 None:intergenic
CCCCTAGAAGAGGATTATTT+CGG 0.368115 8.3:+23602376 MS.gene90493:CDS
TGGTTTCAAGATGGAGGAAT+TGG 0.375248 8.3:-23601571 None:intergenic
TATATGGTTTGGTATTGGAA+TGG 0.381013 8.3:-23601624 None:intergenic
TGGTTTATATGGTTTGGTAT+TGG 0.385182 8.3:-23601629 None:intergenic
AAGCGCTATTATCTCACGTT+TGG 0.385318 8.3:+23602266 MS.gene90493:CDS
TTAAGATGGTTTATATGGTT+TGG 0.398195 8.3:-23601635 None:intergenic
AGGAAAGTGTTTGTTTAAGA+TGG 0.419599 8.3:-23601649 None:intergenic
TTGGTTGTGACACAGCTTCA+TGG 0.433925 8.3:-23601883 None:intergenic
TAAGGAATCATTACGAGTCT+TGG 0.444628 8.3:+23602515 MS.gene90493:CDS
ATATGGTTTGGTATTGGAAT+GGG 0.445755 8.3:-23601623 None:intergenic
TCAAAAGCAAACGCCGAAGC+TGG 0.446381 8.3:+23602214 MS.gene90493:CDS
GAGGTGTATAGTTTGATTGA+AGG 0.450138 8.3:-23601820 None:intergenic
ACTGGTGGAGATCACTTTGA+TGG 0.452170 8.3:-23601474 None:intergenic
AACTTCTTTCCACCCCTCGC+TGG 0.454179 8.3:+23601680 MS.gene90493:CDS
TCCGAAATAATCCTCTTCTA+GGG 0.454875 8.3:-23602377 None:intergenic
TGGAATCGGAAAAGTTGCTT+TGG 0.455281 8.3:+23602453 MS.gene90493:CDS
GGATTGGTAACAAGTATAAT+GGG 0.461227 8.3:+23602668 MS.gene90493:CDS
GCATCATTTCCCATAGAACT+TGG 0.464131 8.3:-23602809 None:intergenic
GGGAAAATTACAGTGTTTGC+TGG 0.464637 8.3:+23602688 MS.gene90493:CDS
GAGTTGTGATTGTTGTCAAT+TGG 0.482251 8.3:-23601509 None:intergenic
AATTGGTGCTTGAATTGTAC+TGG 0.484057 8.3:-23601492 None:intergenic
GAAAAGAAGGCCTGCTTGGA+TGG 0.486070 8.3:-23601591 None:intergenic
ATCCCACTTCGAACTGCCAC+AGG 0.501391 8.3:-23602649 None:intergenic
TGGGTTGTTGGTGGAAAAGA+AGG 0.501427 8.3:-23601604 None:intergenic
GTTCCACTTCCAAGTTCTAT+GGG 0.509409 8.3:+23602800 MS.gene90493:CDS
GTGGCAGTTCGAAGTGGGAT+TGG 0.513271 8.3:+23602652 MS.gene90493:CDS
GGGTGGAAAGAAGTTGAGAG+TGG 0.516551 8.3:-23601672 None:intergenic
GTATTGGAATGGGTTGTTGG+TGG 0.516749 8.3:-23601613 None:intergenic
TCCATCATTTGCAACAACCT+TGG 0.522468 8.3:+23601749 MS.gene90493:CDS
CCACTTGGAGGACTACCACT+TGG 0.530738 8.3:-23602559 None:intergenic
ACCAAGGTTGTTGCAAATGA+TGG 0.532824 8.3:-23601750 None:intergenic
TTTCCCATAGAACTTGGAAG+TGG 0.534045 8.3:-23602803 None:intergenic
GAGGTTCTAGAGTATGGAAT+CGG 0.537759 8.3:+23602439 MS.gene90493:CDS
TAGCAATCACTTGTTGTCCA+AGG 0.538515 8.3:+23602590 MS.gene90493:CDS
CTGCTTGGATGGTTTCAAGA+TGG 0.545156 8.3:-23601580 None:intergenic
GAAAATTGAATTAACACCAT+GGG 0.549693 8.3:+23601544 MS.gene90493:CDS
GGTGGAAAAGAAGGCCTGCT+TGG 0.549803 8.3:-23601595 None:intergenic
GCTAACGAATTACCACTTGG+AGG 0.558087 8.3:-23602571 None:intergenic
CATCTTGAAACCATCCAAGC+AGG 0.563003 8.3:+23601581 MS.gene90493:CDS
ACAACCAACTCTAGGAGTCC+AGG 0.567424 8.3:+23601898 MS.gene90493:CDS
TCTAGGGGCGGAATCAACCT+TGG 0.569302 8.3:-23602361 None:intergenic
GTGTTTGCTGGTGCTGAAGA+AGG 0.574224 8.3:+23602700 MS.gene90493:CDS
CTACCACTTGGAAACAACCT+TGG 0.580849 8.3:-23602547 None:intergenic
GTTGATTCCGCCCCTAGAAG+AGG 0.580936 8.3:+23602366 MS.gene90493:CDS
AGCCTGTGGCAGTTCGAAGT+GGG 0.585379 8.3:+23602647 MS.gene90493:CDS
GCTGTGTCACAACCAACTCT+AGG 0.587882 8.3:+23601890 MS.gene90493:CDS
CTTGGATGGTTTCAAGATGG+AGG 0.589594 8.3:-23601577 None:intergenic
TAGAACTTGGAAGTGGAACA+TGG 0.589628 8.3:-23602796 None:intergenic
TGGAAAGAAGTTGAGAGTGG+AGG 0.590316 8.3:-23601669 None:intergenic
ACATAAGAAACATCTTCTTG+TGG 0.596961 8.3:-23602319 None:intergenic
CCGAAATAATCCTCTTCTAG+GGG 0.597381 8.3:-23602376 None:intergenic
AGTGCAAAATACAACGGTCG+TGG 0.601343 8.3:+23601793 MS.gene90493:CDS
TGGAGGAATTGGAGATCCCA+TGG 0.604811 8.3:-23601560 None:intergenic
GTTACCTGGACTCCTAGAGT+TGG 0.609941 8.3:-23601902 None:intergenic
AGAAAATTGAATTAACACCA+TGG 0.610904 8.3:+23601543 MS.gene90493:CDS
TGTTTATAGGTGTTAGACCA+AGG 0.611467 8.3:+23602344 MS.gene90493:CDS
ACAAGACGACCAGCGAGGGG+TGG 0.619470 8.3:-23601689 None:intergenic
AAGCCTGTGGCAGTTCGAAG+TGG 0.622477 8.3:+23602646 MS.gene90493:CDS
ACTGTTGTGAGGATGAAAGC+AGG 0.624888 8.3:+23602415 MS.gene90493:CDS
TATTACAAGACGACCAGCGA+GGG 0.630336 8.3:-23601693 None:intergenic
AAATAATCCTCTTCTAGGGG+CGG 0.632202 8.3:-23602373 None:intergenic
GGATTATTTCGGAAACGCAG+TGG 0.636378 8.3:+23602387 MS.gene90493:CDS
TTATTTCGGAAACGCAGTGG+TGG 0.651714 8.3:+23602390 MS.gene90493:CDS
ATTGCTAACGAATTACCACT+TGG 0.655593 8.3:-23602574 None:intergenic
TGGTGCTTGAATTGTACTGG+TGG 0.661054 8.3:-23601489 None:intergenic
TGTGAGGATGAAAGCAGGAG+AGG 0.662636 8.3:+23602420 MS.gene90493:CDS
ACACCAAGGTTGTTTCCAAG+TGG 0.665409 8.3:+23602544 MS.gene90493:CDS
ATTACAAGACGACCAGCGAG+GGG 0.669009 8.3:-23601692 None:intergenic
TTTCACAAAGGAGAAAATCG+CGG 0.682651 8.3:+23602183 MS.gene90493:intron
CAGTGGTGGTTACTGTTGTG+AGG 0.691041 8.3:+23602404 MS.gene90493:CDS
GTTGCAAATGATGGAACATG+TGG 0.705405 8.3:-23601741 None:intergenic
CCAAGTGGTAGTCCTCCAAG+TGG 0.727113 8.3:+23602559 MS.gene90493:CDS
TGCCACAGTGCAAAATACAA+CGG 0.729193 8.3:+23601787 MS.gene90493:CDS
CATGGACGAATAGTGCACCA+AGG 0.738412 8.3:-23601766 None:intergenic
TTATTACAAGACGACCAGCG+AGG 0.755763 8.3:-23601694 None:intergenic
AGTCTTGGTTAAGAACACCA+AGG 0.764465 8.3:+23602530 MS.gene90493:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACATTCTGATAAGATAATA+AGG + chr8.3:23602497-23602516 MS.gene90493:CDS 20.0%
!!! TTTGTTTAAGATGGTTTATA+TGG - chr8.3:23601643-23601662 None:intergenic 20.0%
! AAGAATCTTTCATTTCACAA+AGG + chr8.3:23602171-23602190 MS.gene90493:intron 25.0%
! AGAAAATTGAATTAACACCA+TGG + chr8.3:23601543-23601562 MS.gene90493:CDS 25.0%
! ATTGGAATTTCAATATCGTT+TGG - chr8.3:23602076-23602095 None:intergenic 25.0%
! GAAAATTGAATTAACACCAT+GGG + chr8.3:23601544-23601563 MS.gene90493:CDS 25.0%
! TGTGAAATGAAAGATTCTTT+CGG - chr8.3:23602170-23602189 None:intergenic 25.0%
! TTAAGATGGTTTATATGGTT+TGG - chr8.3:23601638-23601657 None:intergenic 25.0%
! TTGGAATTTCAATATCGTTT+GGG - chr8.3:23602075-23602094 None:intergenic 25.0%
!! TTGTCTTTCTTATGAGATTT+TGG + chr8.3:23602741-23602760 MS.gene90493:CDS 25.0%
!!! GTTTCTTATGTGTTGTTTAT+AGG + chr8.3:23602331-23602350 MS.gene90493:CDS 25.0%
AAAACAGAATCACAAAACCA+AGG + chr8.3:23602112-23602131 MS.gene90493:intron 30.0%
ACATAAGAAACATCTTCTTG+TGG - chr8.3:23602322-23602341 None:intergenic 30.0%
TTTGGCATTTCGTTAATTCA+TGG + chr8.3:23601990-23602009 MS.gene90493:intron 30.0%
! GGATTGGTAACAAGTATAAT+GGG + chr8.3:23602668-23602687 MS.gene90493:CDS 30.0%
! TCTTTCTTATGAGATTTTGG+AGG + chr8.3:23602744-23602763 MS.gene90493:CDS 30.0%
!! AAGGGAGTTTTGATATTTTG+TGG - chr8.3:23602036-23602055 None:intergenic 30.0%
!! AGGAAAGTGTTTGTTTAAGA+TGG - chr8.3:23601652-23601671 None:intergenic 30.0%
!! AGGGAGTTTTGATATTTTGT+GGG - chr8.3:23602035-23602054 None:intergenic 30.0%
!! ATATGGTTTGGTATTGGAAT+GGG - chr8.3:23601626-23601645 None:intergenic 30.0%
!! TATATGGTTTGGTATTGGAA+TGG - chr8.3:23601627-23601646 None:intergenic 30.0%
!! TGGTTTATATGGTTTGGTAT+TGG - chr8.3:23601632-23601651 None:intergenic 30.0%
!! TTTTTGGCAATGATTTTGGT+TGG + chr8.3:23602620-23602639 MS.gene90493:CDS 30.0%
!!! GATGTTTTTGGCAATGATTT+TGG + chr8.3:23602616-23602635 MS.gene90493:CDS 30.0%
!!! GTGATTCTGTTTTTGTGAAA+GGG - chr8.3:23602105-23602124 None:intergenic 30.0%
!!! TGTGATTCTGTTTTTGTGAA+AGG - chr8.3:23602106-23602125 None:intergenic 30.0%
!!! TTTTGGCAATGATTTTGGTT+GGG + chr8.3:23602621-23602640 MS.gene90493:CDS 30.0%
AAAGATTCTTTCGGCTAACT+TGG - chr8.3:23602161-23602180 None:intergenic 35.0%
ATTGCTAACGAATTACCACT+TGG - chr8.3:23602577-23602596 None:intergenic 35.0%
GAGGTGTATAGTTTGATTGA+AGG - chr8.3:23601823-23601842 None:intergenic 35.0%
GAGTTGTGATTGTTGTCAAT+TGG - chr8.3:23601512-23601531 None:intergenic 35.0%
GGTTAAGTGGAAAAAATGAG+CGG - chr8.3:23601853-23601872 None:intergenic 35.0%
TAAGGAATCATTACGAGTCT+TGG + chr8.3:23602515-23602534 MS.gene90493:CDS 35.0%
TCATGGTCACAAATCTCAAA+AGG + chr8.3:23602007-23602026 MS.gene90493:intron 35.0%
TCCGAAATAATCCTCTTCTA+GGG - chr8.3:23602380-23602399 None:intergenic 35.0%
TGTTTATAGGTGTTAGACCA+AGG + chr8.3:23602344-23602363 MS.gene90493:CDS 35.0%
TTCCGAAATAATCCTCTTCT+AGG - chr8.3:23602381-23602400 None:intergenic 35.0%
TTGCCAAAAACATCAAACCT+TGG - chr8.3:23602610-23602629 None:intergenic 35.0%
TTTCACAAAGGAGAAAATCG+CGG + chr8.3:23602183-23602202 MS.gene90493:intron 35.0%
! AATTGGTGCTTGAATTGTAC+TGG - chr8.3:23601495-23601514 None:intergenic 35.0%
! GGGATTGGTAACAAGTATAA+TGG + chr8.3:23602667-23602686 MS.gene90493:CDS 35.0%
! TGTCCAAGGTTTGATGTTTT+TGG + chr8.3:23602604-23602623 MS.gene90493:CDS 35.0%
! TTTGTGAAAGGGAAGTGTAT+TGG - chr8.3:23602094-23602113 None:intergenic 35.0%
!! TATGAGATTTTGGAGGCTTT+GGG + chr8.3:23602751-23602770 MS.gene90493:CDS 35.0%
!! TTATGAGATTTTGGAGGCTT+TGG + chr8.3:23602750-23602769 MS.gene90493:CDS 35.0%
!!! AGGTTTTTTGCAACGAACAA+CGG - chr8.3:23602296-23602315 None:intergenic 35.0%
!!! TTTGGCAATGATTTTGGTTG+GGG + chr8.3:23602622-23602641 MS.gene90493:CDS 35.0%
AAAAATGAGCGGACAATTGG+AGG - chr8.3:23601842-23601861 None:intergenic 40.0%
AACTCCCTTCATTTCAACGT+TGG + chr8.3:23602047-23602066 MS.gene90493:intron 40.0%
AAGCGCTATTATCTCACGTT+TGG + chr8.3:23602266-23602285 MS.gene90493:CDS 40.0%
ACCAAGGTTGTTGCAAATGA+TGG - chr8.3:23601753-23601772 None:intergenic 40.0%
AGTCTTGGTTAAGAACACCA+AGG + chr8.3:23602530-23602549 MS.gene90493:CDS 40.0%
CCCCTAGAAGAGGATTATTT+CGG + chr8.3:23602376-23602395 MS.gene90493:CDS 40.0%
CCGAAATAATCCTCTTCTAG+GGG - chr8.3:23602379-23602398 None:intergenic 40.0%
GAAACATCTTCTTGTGGATC+AGG - chr8.3:23602316-23602335 None:intergenic 40.0%
GGAAAAAATGAGCGGACAAT+TGG - chr8.3:23601845-23601864 None:intergenic 40.0%
GGAACCAACGTTGAAATGAA+GGG - chr8.3:23602054-23602073 None:intergenic 40.0%
GGATTCTAGGCTTTCTTCTA+AGG - chr8.3:23602140-23602159 None:intergenic 40.0%
GGGAAAATTACAGTGTTTGC+TGG + chr8.3:23602688-23602707 MS.gene90493:CDS 40.0%
GTTCCACTTCCAAGTTCTAT+GGG + chr8.3:23602800-23602819 MS.gene90493:CDS 40.0%
TAGAACTTGGAAGTGGAACA+TGG - chr8.3:23602799-23602818 None:intergenic 40.0%
TCCATCATTTGCAACAACCT+TGG + chr8.3:23601749-23601768 MS.gene90493:CDS 40.0%
TGCCACAGTGCAAAATACAA+CGG + chr8.3:23601787-23601806 MS.gene90493:CDS 40.0%
TGGAATCGGAAAAGTTGCTT+TGG + chr8.3:23602453-23602472 MS.gene90493:CDS 40.0%
TGGTTTCAAGATGGAGGAAT+TGG - chr8.3:23601574-23601593 None:intergenic 40.0%
TGTTCCACTTCCAAGTTCTA+TGG + chr8.3:23602799-23602818 MS.gene90493:CDS 40.0%
TTTCCCATAGAACTTGGAAG+TGG - chr8.3:23602806-23602825 None:intergenic 40.0%
TTTCGGCTAACTTGGATTCT+AGG - chr8.3:23602153-23602172 None:intergenic 40.0%
! AAATAATCCTCTTCTAGGGG+CGG - chr8.3:23602376-23602395 None:intergenic 40.0%
! AGTTTTCTCTGTTCCAGCTT+CGG - chr8.3:23602230-23602249 None:intergenic 40.0%
! CAGCTTCATGGTTTTTAACG+CGG - chr8.3:23601874-23601893 None:intergenic 40.0%
! GTTGCAAATGATGGAACATG+TGG - chr8.3:23601744-23601763 None:intergenic 40.0%
! TGGTTTTTAACGCGGTTAAG+TGG - chr8.3:23601866-23601885 None:intergenic 40.0%
! TTGTATTTTGCACTGTGGCA+TGG - chr8.3:23601787-23601806 None:intergenic 40.0%
!! ATGAGATTTTGGAGGCTTTG+GGG + chr8.3:23602752-23602771 MS.gene90493:CDS 40.0%
!! GAGGTTCTAGAGTATGGAAT+CGG + chr8.3:23602439-23602458 MS.gene90493:CDS 40.0%
!! TAGCAATCACTTGTTGTCCA+AGG + chr8.3:23602590-23602609 MS.gene90493:CDS 40.0%
!! TTGGTATTGGAATGGGTTGT+TGG - chr8.3:23601619-23601638 None:intergenic 40.0%
ACTGGTGGAGATCACTTTGA+TGG - chr8.3:23601477-23601496 None:intergenic 45.0%
ACTGTTGTGAGGATGAAAGC+AGG + chr8.3:23602415-23602434 MS.gene90493:CDS 45.0%
AGTGCAAAATACAACGGTCG+TGG + chr8.3:23601793-23601812 MS.gene90493:CDS 45.0%
CATCTTGAAACCATCCAAGC+AGG + chr8.3:23601581-23601600 MS.gene90493:CDS 45.0%
CTACCACTTGGAAACAACCT+TGG - chr8.3:23602550-23602569 None:intergenic 45.0%
CTGCTTGGATGGTTTCAAGA+TGG - chr8.3:23601583-23601602 None:intergenic 45.0%
CTTGGATGGTTTCAAGATGG+AGG - chr8.3:23601580-23601599 None:intergenic 45.0%
GCTAACGAATTACCACTTGG+AGG - chr8.3:23602574-23602593 None:intergenic 45.0%
GGATTATTTCGGAAACGCAG+TGG + chr8.3:23602387-23602406 MS.gene90493:CDS 45.0%
GGGAACCAACGTTGAAATGA+AGG - chr8.3:23602055-23602074 None:intergenic 45.0%
TATTACAAGACGACCAGCGA+GGG - chr8.3:23601696-23601715 None:intergenic 45.0%
TGGAAAGAAGTTGAGAGTGG+AGG - chr8.3:23601672-23601691 None:intergenic 45.0%
TGGTGCTTGAATTGTACTGG+TGG - chr8.3:23601492-23601511 None:intergenic 45.0%
TTATTACAAGACGACCAGCG+AGG - chr8.3:23601697-23601716 None:intergenic 45.0%
TTATTTCGGAAACGCAGTGG+TGG + chr8.3:23602390-23602409 MS.gene90493:CDS 45.0%
TTGGTTGTGACACAGCTTCA+TGG - chr8.3:23601886-23601905 None:intergenic 45.0%
! AACTCTAGGAGTCCAGGTAA+CGG + chr8.3:23601904-23601923 MS.gene90493:intron 45.0%
! ACACCAAGGTTGTTTCCAAG+TGG + chr8.3:23602544-23602563 MS.gene90493:CDS 45.0%
! GACCGTTGTATTTTGCACTG+TGG - chr8.3:23601792-23601811 None:intergenic 45.0%
! TGGGTTGTTGGTGGAAAAGA+AGG - chr8.3:23601607-23601626 None:intergenic 45.0%
!! GTATTGGAATGGGTTGTTGG+TGG - chr8.3:23601616-23601635 None:intergenic 45.0%
ATTACAAGACGACCAGCGAG+GGG - chr8.3:23601695-23601714 None:intergenic 50.0%
CAGTGGTGGTTACTGTTGTG+AGG + chr8.3:23602404-23602423 MS.gene90493:CDS 50.0%
CATGGACGAATAGTGCACCA+AGG - chr8.3:23601769-23601788 None:intergenic 50.0%
GAAAAGAAGGCCTGCTTGGA+TGG - chr8.3:23601594-23601613 None:intergenic 50.0%
GCTGTGTCACAACCAACTCT+AGG + chr8.3:23601890-23601909 MS.gene90493:CDS 50.0%
GGGGAATGACACTGAGTTCA+TGG + chr8.3:23602771-23602790 MS.gene90493:CDS 50.0%
GGGTGGAAAGAAGTTGAGAG+TGG - chr8.3:23601675-23601694 None:intergenic 50.0%
GTTACCTGGACTCCTAGAGT+TGG - chr8.3:23601905-23601924 None:intergenic 50.0%
TCAAAAGCAAACGCCGAAGC+TGG + chr8.3:23602214-23602233 MS.gene90493:CDS 50.0%
TGAAGTGACGGCGTCTTCAT+TGG + chr8.3:23601929-23601948 MS.gene90493:intron 50.0%
TGCAACCACGTTGTTGCAGA+CGG + chr8.3:23601959-23601978 MS.gene90493:intron 50.0%
TGGAGGAATTGGAGATCCCA+TGG - chr8.3:23601563-23601582 None:intergenic 50.0%
TGTGAGGATGAAAGCAGGAG+AGG + chr8.3:23602420-23602439 MS.gene90493:CDS 50.0%
! ACAACCAACTCTAGGAGTCC+AGG + chr8.3:23601898-23601917 MS.gene90493:CDS 50.0%
! GGCTTTCTTCTAAGGAGCCT+TGG - chr8.3:23602132-23602151 None:intergenic 50.0%
! GTGTTTGCTGGTGCTGAAGA+AGG + chr8.3:23602700-23602719 MS.gene90493:CDS 50.0%
! TTGCAGACGGCAAGTCGTTT+TGG + chr8.3:23601972-23601991 MS.gene90493:intron 50.0%
!! CAGGTAACGGAGTGAAGTGA+CGG + chr8.3:23601917-23601936 MS.gene90493:intron 50.0%
!! GCAGGAGAGGTTCTAGAGTA+TGG + chr8.3:23602433-23602452 MS.gene90493:CDS 50.0%
!! TTTTGGTTGGGGAAAGCCTG+TGG + chr8.3:23602633-23602652 MS.gene90493:CDS 50.0%
AACTTCTTTCCACCCCTCGC+TGG + chr8.3:23601680-23601699 MS.gene90493:CDS 55.0%
AAGCCTGTGGCAGTTCGAAG+TGG + chr8.3:23602646-23602665 MS.gene90493:CDS 55.0%
ACTTGCCGTCTGCAACAACG+TGG - chr8.3:23601967-23601986 None:intergenic 55.0%
AGCCTGTGGCAGTTCGAAGT+GGG + chr8.3:23602647-23602666 MS.gene90493:CDS 55.0%
ATCCCACTTCGAACTGCCAC+AGG - chr8.3:23602652-23602671 None:intergenic 55.0%
CCAAGTGGTAGTCCTCCAAG+TGG + chr8.3:23602559-23602578 MS.gene90493:CDS 55.0%
CCACTTGGAGGACTACCACT+TGG - chr8.3:23602562-23602581 None:intergenic 55.0%
CGTCACTTCACTCCGTTACC+TGG - chr8.3:23601919-23601938 None:intergenic 55.0%
GGTGGAAAAGAAGGCCTGCT+TGG - chr8.3:23601598-23601617 None:intergenic 55.0%
GTGGCAGTTCGAAGTGGGAT+TGG + chr8.3:23602652-23602671 MS.gene90493:CDS 55.0%
TCTAGGGGCGGAATCAACCT+TGG - chr8.3:23602364-23602383 None:intergenic 55.0%
! GTTGATTCCGCCCCTAGAAG+AGG + chr8.3:23602366-23602385 MS.gene90493:CDS 55.0%
ACAAGACGACCAGCGAGGGG+TGG - chr8.3:23601692-23601711 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 23601467 23602828 23601467 ID=MS.gene90493
chr8.3 mRNA 23601467 23602828 23601467 ID=MS.gene90493.t1;Parent=MS.gene90493
chr8.3 exon 23601467 23601919 23601467 ID=MS.gene90493.t1.exon1;Parent=MS.gene90493.t1
chr8.3 CDS 23601467 23601919 23601467 ID=cds.MS.gene90493.t1;Parent=MS.gene90493.t1
chr8.3 exon 23602193 23602828 23602193 ID=MS.gene90493.t1.exon2;Parent=MS.gene90493.t1
chr8.3 CDS 23602193 23602828 23602193 ID=cds.MS.gene90493.t1;Parent=MS.gene90493.t1
Gene Sequence

>MS.gene90493

ATGGGTTCCATCAAAGTGATCTCCACCAGTACAATTCAAGCACCAATTGACAACAATCACAACTCGTCAGATTATCAGAAAATTGAATTAACACCATGGGATCTCCAATTCCTCCATCTTGAAACCATCCAAGCAGGCCTTCTTTTCCACCAACAACCCATTCCAATACCAAACCATATAAACCATCTTAAACAAACACTTTCCTCCACTCTCAACTTCTTTCCACCCCTCGCTGGTCGTCTTGTAATAACGCGAGACGATGAACATAACAACGCCACATGTTCCATCATTTGCAACAACCTTGGTGCACTATTCGTCCATGCCACAGTGCAAAATACAACGGTCGTGGATATCCTTCAATCAAACTATACACCTCCAATTGTCCGCTCATTTTTTCCACTTAACCGCGTTAAAAACCATGAAGCTGTGTCACAACCAACTCTAGGAGTCCAGGTAACGGAGTGAAGTGACGGCGTCTTCATTGGCTTCACATGCAACCACGTTGTTGCAGACGGCAAGTCGTTTTGGCATTTCGTTAATTCATGGTCACAAATCTCAAAAGGTTCCCACAAAATATCAAAACTCCCTTCATTTCAACGTTGGTTCCCAAACGATATTGAAATTCCAATACACTTCCCTTTCACAAAAACAGAATCACAAAACCAAGGCTCCTTAGAAGAAAGCCTAGAATCCAAGTTAGCCGAAAGAATCTTTCATTTCACAAAGGAGAAAATCGCGGAACTCAAATCAAAAGCAAACGCCGAAGCTGGAACAGAGAAAACTAAAATAACTTCATTTCAAGCGCTATTATCTCACGTTTGGCGTTCCGTTGTTCGTTGCAAAAAACCTGATCCACAAGAAGATGTTTCTTATGTGTTGTTTATAGGTGTTAGACCAAGGTTGATTCCGCCCCTAGAAGAGGATTATTTCGGAAACGCAGTGGTGGTTACTGTTGTGAGGATGAAAGCAGGAGAGGTTCTAGAGTATGGAATCGGAAAAGTTGCTTTGGAGATAAACAAGATGATTTCGTTACATTCTGATAAGATAATAAGGAATCATTACGAGTCTTGGTTAAGAACACCAAGGTTGTTTCCAAGTGGTAGTCCTCCAAGTGGTAATTCGTTAGCAATCACTTGTTGTCCAAGGTTTGATGTTTTTGGCAATGATTTTGGTTGGGGAAAGCCTGTGGCAGTTCGAAGTGGGATTGGTAACAAGTATAATGGGAAAATTACAGTGTTTGCTGGTGCTGAAGAAGGTAGTATAAATGTTGAATTTTGTCTTTCTTATGAGATTTTGGAGGCTTTGGGGAATGACACTGAGTTCATGGATCCATGTTCCACTTCCAAGTTCTATGGGAAATGA
Protein sequence

>MS.gene90493.t1

MGSIKVISTSTIQAPIDNNHNSSDYQKIELTPWDLQFLHLETIQAGLLFHQQPIPIPNHINHLKQTLSSTLNFFPPLAGRLVITRDDEHNNATCSIICNNLGALFVHATVQNTTVVDILQSNYTPPIVRSFFPLNRVKNHEAVSQPTLGVQEKIAELKSKANAEAGTEKTKITSFQALLSHVWRSVVRCKKPDPQEDVSYVLFIGVRPRLIPPLEEDYFGNAVVVTVVRMKAGEVLEYGIGKVALEINKMISLHSDKIIRNHYESWLRTPRLFPSGSPPSGNSLAITCCPRFDVFGNDFGWGKPVAVRSGIGNKYNGKITVFAGAEEGSINVEFCLSYEILEALGNDTEFMDPCSTSKFYGK