AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066658

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066658.t1 MTR_4g022670 84.842 442 67 0 1 442 1 442 0.0 792
MS.gene066658.t1 MTR_4g022800 83.070 443 74 1 1 442 1 443 0.0 778
MS.gene066658.t1 MTR_4g022720 81.900 442 74 1 1 442 1 436 0.0 755
MS.gene066658.t1 MTR_7g026860 79.864 442 86 1 1 442 1 439 0.0 747
MS.gene066658.t1 MTR_4g022760 81.222 442 39 1 1 442 1 398 0.0 729
MS.gene066658.t1 MTR_4g022825 73.469 441 116 1 1 441 1 440 0.0 677
MS.gene066658.t1 MTR_3g088420 67.647 442 135 2 1 442 1 434 0.0 604
MS.gene066658.t1 MTR_3g026310 66.370 449 144 3 1 442 1 449 0.0 593
MS.gene066658.t1 MTR_0190s0020 57.301 452 172 7 1 441 1 442 1.86e-179 508
MS.gene066658.t1 MTR_8g041770 57.489 454 169 7 1 442 1 442 8.68e-176 499
MS.gene066658.t1 MTR_4g094842 56.427 459 167 10 1 439 1 446 3.22e-169 483
MS.gene066658.t1 MTR_4g094835 53.407 455 181 8 1 437 1 442 3.76e-161 462
MS.gene066658.t1 MTR_4g022290 51.193 461 185 13 1 439 1 443 2.92e-148 429
MS.gene066658.t1 MTR_4g094838 50.328 457 175 9 4 437 1 428 2.39e-147 427
MS.gene066658.t1 MTR_8g079710 51.435 453 182 11 1 432 1 436 3.56e-146 424
MS.gene066658.t1 MTR_4g094828 50.325 461 198 12 1 441 1 450 3.68e-143 417
MS.gene066658.t1 MTR_6g016815 51.810 442 139 8 1 442 15 382 1.63e-141 410
MS.gene066658.t1 MTR_4g094832 55.875 383 142 6 69 437 57 426 1.10e-139 407
MS.gene066658.t1 MTR_3g101930 40.359 446 232 11 16 440 22 454 1.12e-95 295
MS.gene066658.t1 MTR_3g101900 40.183 438 235 11 18 441 24 448 6.30e-95 293
MS.gene066658.t1 MTR_7g026850 77.576 165 36 1 23 187 7 170 1.85e-91 275
MS.gene066658.t1 MTR_3g102020 38.914 442 243 10 16 441 21 451 2.46e-91 284
MS.gene066658.t1 MTR_7g026770 68.687 198 34 1 141 338 132 301 7.65e-89 272
MS.gene066658.t1 MTR_3g101960 38.761 436 241 10 16 436 21 445 2.37e-88 276
MS.gene066658.t1 MTR_8g040760 62.911 213 72 3 1 207 1 212 2.93e-87 265
MS.gene066658.t1 MTR_3g101970 37.585 439 237 10 16 440 21 436 2.77e-86 270
MS.gene066658.t1 MTR_3g102010 36.961 441 252 10 16 441 22 451 1.72e-83 264
MS.gene066658.t1 MTR_8g040780 57.407 216 88 2 225 436 12 227 5.31e-78 243
MS.gene066658.t1 MTR_3g070170 31.458 480 270 13 2 441 10 470 7.22e-59 200
MS.gene066658.t1 MTR_4g015180 32.033 487 254 14 16 442 19 488 2.47e-58 199
MS.gene066658.t1 MTR_5g081960 29.461 482 262 11 16 437 19 482 3.50e-56 193
MS.gene066658.t1 MTR_4g094825 50.265 189 85 5 17 199 84 269 7.44e-52 179
MS.gene066658.t1 MTR_4g094825 52.532 158 62 2 286 441 245 391 1.73e-45 162
MS.gene066658.t1 MTR_3g102030 35.161 310 162 5 90 397 48 320 3.37e-50 173
MS.gene066658.t1 MTR_6g045553 29.684 475 276 13 4 437 14 471 6.70e-50 176
MS.gene066658.t1 MTR_7g026800 52.326 172 51 4 211 380 18 160 1.10e-46 159
MS.gene066658.t1 MTR_6g034495 58.955 134 47 2 54 179 28 161 3.82e-46 157
MS.gene066658.t1 MTR_4g022780 51.149 174 54 4 211 382 19 163 9.38e-46 157
MS.gene066658.t1 MTR_6g034485 61.364 132 43 2 189 320 2 125 5.76e-45 153
MS.gene066658.t1 MTR_7g026830 78.571 84 18 0 83 166 15 98 5.11e-41 141
MS.gene066658.t1 MTR_7g026780 63.964 111 16 2 332 442 26 112 8.25e-39 136
MS.gene066658.t1 MTR_8g075610 26.385 379 260 11 55 428 47 411 1.14e-30 122
MS.gene066658.t1 MTR_4g007540 26.817 399 237 14 55 428 56 424 1.78e-28 117
MS.gene066658.t1 MTR_2g105330 27.177 379 250 13 55 423 56 418 6.74e-28 115
MS.gene066658.t1 MTR_2g064495 27.177 379 250 13 55 423 56 418 8.68e-28 115
MS.gene066658.t1 MTR_7g026870 85.455 55 8 0 297 351 97 151 5.48e-27 105
MS.gene066658.t1 MTR_8g096530 28.261 414 240 19 49 432 53 439 2.03e-25 108
MS.gene066658.t1 MTR_7g026880 78.846 52 11 0 390 441 2 53 1.72e-24 95.9
MS.gene066658.t1 MTR_3g098980 28.763 372 196 18 49 393 60 389 1.33e-23 102
MS.gene066658.t1 MTR_3g026950 25.485 412 253 14 48 426 47 437 1.62e-23 102
MS.gene066658.t1 MTR_2g089755 25.056 443 288 15 24 432 37 469 2.39e-22 99.8
MS.gene066658.t1 MTR_4g013050 27.273 363 198 12 50 393 48 363 4.11e-22 98.2
MS.gene066658.t1 MTR_2g089650 24.940 417 265 17 38 420 43 445 7.60e-22 98.2
MS.gene066658.t1 MTR_8g012750 25.231 432 270 15 3 392 2 422 7.28e-20 92.0
MS.gene066658.t1 MTR_3g049150 25.313 399 257 16 52 426 52 433 1.11e-19 91.3
MS.gene066658.t1 MTR_3g028210 24.783 460 288 16 4 423 8 449 1.02e-18 88.6
MS.gene066658.t1 MTR_7g014250 25.301 415 251 16 19 396 21 413 1.05e-18 88.6
MS.gene066658.t1 MTR_6g015830 25.949 474 260 19 10 432 21 454 1.50e-18 88.2
MS.gene066658.t1 MTR_7g114490 24.385 447 278 18 25 439 24 442 1.60e-18 88.2
MS.gene066658.t1 MTR_7g027175 24.936 389 262 13 49 428 62 429 2.52e-18 87.0
MS.gene066658.t1 MTR_7g014230 27.506 429 226 20 18 396 22 415 3.64e-18 87.0
MS.gene066658.t1 MTR_7g014330 25.359 418 249 16 18 396 20 413 4.47e-18 86.7
MS.gene066658.t1 MTR_2g089765 25.000 424 249 16 17 392 32 434 7.26e-18 86.3
MS.gene066658.t1 MTR_7g114470 23.394 436 276 16 25 428 25 434 7.38e-18 85.9
MS.gene066658.t1 MTR_1g081660 23.645 406 234 16 24 393 31 396 7.45e-18 85.9
MS.gene066658.t1 MTR_3g086020 26.304 460 268 20 4 441 5 415 9.09e-18 85.5
MS.gene066658.t1 MTR_2g089670 25.974 385 240 16 38 392 60 429 1.31e-17 85.5
MS.gene066658.t1 MTR_3g087640 25.474 475 298 15 1 432 5 466 3.47e-17 84.0
MS.gene066658.t1 MTR_6g015315 27.899 276 173 8 138 395 140 407 4.03e-17 83.6
MS.gene066658.t1 MTR_5g076700 23.810 441 277 17 25 433 24 437 7.68e-17 82.8
MS.gene066658.t1 MTR_7g114550 24.717 441 272 17 25 433 24 436 8.15e-17 82.8
MS.gene066658.t1 MTR_6g015850 25.607 453 285 16 10 432 21 451 1.26e-16 82.0
MS.gene066658.t1 MTR_7g015860 21.689 438 266 15 4 393 5 413 1.74e-16 81.6
MS.gene066658.t1 MTR_1g040175 25.344 363 234 16 55 407 64 399 2.12e-16 81.6
MS.gene066658.t1 MTR_5g090810 23.896 385 238 12 43 393 49 412 2.31e-16 81.3
MS.gene066658.t1 MTR_2g089735 25.352 426 271 18 41 432 43 455 2.48e-16 81.3
MS.gene066658.t1 MTR_7g084940 27.401 354 215 16 53 392 67 392 3.25e-16 80.9
MS.gene066658.t1 MTR_7g114520 24.041 391 252 14 25 393 25 392 3.32e-16 80.9
MS.gene066658.t1 MTR_5g090830 24.156 385 237 12 43 393 49 412 6.99e-16 80.1
MS.gene066658.t1 MTR_1g040215 25.831 391 242 17 25 393 23 387 7.80e-16 79.7
MS.gene066658.t1 MTR_4g097090 25.064 391 255 13 55 422 65 440 1.21e-15 79.3
MS.gene066658.t1 MTR_5g090880 24.228 421 265 15 55 441 67 467 5.41e-15 77.4
MS.gene066658.t1 MTR_8g079970 24.942 429 260 18 4 405 3 396 1.39e-14 75.9
MS.gene066658.t1 MTR_7g114560 24.717 441 272 19 25 433 24 436 1.88e-14 75.5
MS.gene066658.t1 MTR_4g127650 24.552 391 256 12 55 422 66 440 4.59e-14 74.3
MS.gene066658.t1 MTR_3g112180 24.855 346 239 10 52 391 58 388 1.13e-13 73.2
MS.gene066658.t1 MTR_7g014360 28.214 280 154 10 138 396 90 343 1.20e-13 72.8
MS.gene066658.t1 MTR_3g071320 26.019 319 213 10 27 334 37 343 2.42e-13 72.0
MS.gene066658.t1 MTR_1g040315 27.243 301 184 13 25 309 23 304 2.51e-13 71.6
MS.gene066658.t1 MTR_7g013850 23.691 401 241 15 43 396 54 436 8.80e-13 70.5
MS.gene066658.t1 MTR_7g014200 24.242 429 255 17 15 396 19 424 3.85e-12 68.6
MS.gene066658.t1 MTR_3g465990 25.632 277 186 8 55 324 68 331 6.17e-12 67.8
MS.gene066658.t1 MTR_7g086000 24.694 409 243 17 58 426 63 446 6.75e-12 67.4
MS.gene066658.t1 MTR_7g084910 25.830 271 166 11 53 308 68 318 1.02e-11 67.0
MS.gene066658.t1 MTR_7g014610 23.179 453 290 13 15 422 18 457 1.49e-11 66.6
MS.gene066658.t1 MTR_3g049140 28.333 180 111 5 235 399 27 203 1.86e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066658.t1 AT5G67150 39.956 453 245 12 1 437 1 442 7.03e-99 303
MS.gene066658.t1 AT5G67160 39.414 444 238 11 6 441 12 432 5.96e-93 288
MS.gene066658.t1 AT3G50300 40.138 436 235 13 1 422 1 424 6.22e-91 283
MS.gene066658.t1 AT3G50280 36.889 450 262 10 1 438 1 440 1.54e-89 279
MS.gene066658.t1 AT5G42830 37.955 440 249 10 15 441 21 449 1.60e-88 276
MS.gene066658.t1 AT3G50270 38.074 457 255 13 2 441 3 448 4.77e-87 273
MS.gene066658.t1 AT5G07860 37.613 444 242 10 15 440 22 448 2.84e-82 261
MS.gene066658.t1 AT5G07850 38.202 445 246 12 15 442 22 454 1.24e-80 256
MS.gene066658.t1 AT5G38130 36.697 436 248 12 1 422 21 442 7.96e-79 252
MS.gene066658.t1 AT5G07870 38.210 458 236 14 15 442 22 462 1.86e-76 246
MS.gene066658.t1 AT2G39980 31.667 480 256 12 16 442 21 481 1.13e-67 223
MS.gene066658.t1 AT5G01210 32.553 470 254 12 16 441 19 469 3.74e-61 206
MS.gene066658.t1 AT3G50280 36.478 318 186 6 127 438 3 310 8.40e-61 201
MS.gene066658.t1 AT5G23940 30.330 455 287 14 4 437 3 448 5.17e-55 190
MS.gene066658.t1 AT3G50290 39.350 277 149 7 122 390 4 269 5.38e-52 176
MS.gene066658.t1 AT5G48930 28.608 395 233 15 53 425 54 421 9.48e-31 123
MS.gene066658.t1 AT3G48720 28.000 350 216 14 55 393 61 385 8.16e-23 100
MS.gene066658.t1 AT3G29590 24.569 464 288 15 3 432 7 442 5.84e-22 98.2
MS.gene066658.t1 AT5G41040 26.741 359 223 14 49 393 63 395 6.11e-22 98.2
MS.gene066658.t1 AT5G41040 26.741 359 223 14 49 393 79 411 7.36e-22 98.2
MS.gene066658.t1 AT3G29670 23.598 428 275 15 24 422 29 433 8.87e-21 94.7
MS.gene066658.t1 AT5G57840 27.252 444 261 20 11 422 9 422 1.47e-20 94.0
MS.gene066658.t1 AT5G39080 25.607 453 272 21 14 422 14 445 3.02e-20 93.2
MS.gene066658.t1 AT2G19070 24.601 439 282 15 27 430 19 443 3.30e-20 93.2
MS.gene066658.t1 AT3G03480 26.651 439 255 22 25 428 33 439 7.29e-20 92.0
MS.gene066658.t1 AT5G63560 27.374 358 216 15 49 393 55 381 9.37e-20 91.7
MS.gene066658.t1 AT5G61160 26.182 275 177 10 135 393 139 403 1.55e-16 82.0
MS.gene066658.t1 AT5G39090 28.102 274 167 13 135 392 140 399 4.20e-16 80.5
MS.gene066658.t1 AT3G29635 25.191 393 255 15 24 392 29 406 1.39e-15 79.0
MS.gene066658.t1 AT1G28680 24.944 445 261 19 15 432 18 416 1.65e-15 79.0
MS.gene066658.t1 AT5G16410 24.156 385 244 16 41 402 77 436 1.00e-14 76.6
MS.gene066658.t1 AT1G24430 24.760 416 265 15 41 442 45 426 1.23e-14 75.9
MS.gene066658.t1 AT1G03390 25.352 355 232 14 52 393 73 407 1.45e-14 75.9
MS.gene066658.t1 AT2G40230 24.697 413 273 17 1 397 1 391 1.87e-14 75.5
MS.gene066658.t1 AT1G32910 25.627 359 227 15 53 393 63 399 2.62e-14 75.1
MS.gene066658.t1 AT1G28680 25.680 331 201 13 15 325 18 323 3.93e-14 74.3
MS.gene066658.t1 AT1G31490 25.210 357 225 14 49 392 56 383 1.64e-13 72.4
MS.gene066658.t1 AT2G25150 24.234 359 239 13 52 396 64 403 1.83e-13 72.4
MS.gene066658.t1 AT3G30280 22.652 362 232 11 49 393 56 386 2.73e-13 72.0
MS.gene066658.t1 AT5G07080 25.952 289 164 12 56 322 71 331 3.31e-13 71.6
MS.gene066658.t1 AT1G78990 24.501 351 236 12 53 393 65 396 3.81e-13 71.6
MS.gene066658.t1 AT5G17540 23.750 400 240 15 25 393 23 388 5.53e-13 70.9

Find 91 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTCTTCCTTACGAGATTT+TGG 0.108623 4.2:+80090709 MS.gene066658:CDS
CATACGATTTCCTTTCTTTA+TGG 0.108680 4.2:+80090055 MS.gene066658:CDS
CCATTACCAGAAAATTATTT+TGG 0.134239 4.2:+80090347 MS.gene066658:CDS
TCTCCAAGGTTTGATGTTTA+TGG 0.150931 4.2:+80090578 MS.gene066658:CDS
ACTTGTTTCAACCAACAAAA+AGG 0.207656 4.2:+80089545 MS.gene066658:CDS
TAAGAAATCCAAGCTTTCTT+AGG 0.256333 4.2:+80090513 MS.gene066658:CDS
CTAGTTGACGGTATCTTTAT+TGG 0.259204 4.2:+80089885 MS.gene066658:CDS
AGTGTCTCAGCCGTTGGATT+TGG 0.276668 4.2:-80089599 None:intergenic
ATTGTTTGATCACTTGATTT+TGG 0.339606 4.2:-80089492 None:intergenic
GATGTTTATGGTAATGATTT+CGG 0.343194 4.2:+80090590 MS.gene066658:CDS
GTGTCTCAGCCGTTGGATTT+GGG 0.349706 4.2:-80089598 None:intergenic
GGACTTGTTAATGTGTTTGC+TGG 0.362735 4.2:+80090656 MS.gene066658:CDS
AATGAAAGTTGGGGAGTTGT+TGG 0.379006 4.2:+80090397 MS.gene066658:CDS
ACACCTCACGTTGCATTAGT+TGG 0.383509 4.2:-80090771 None:intergenic
GTATGGTTAAAATGAAAGTT+GGG 0.388503 4.2:+80090387 MS.gene066658:CDS
TGTGGCATTTCATCAATTCA+TGG 0.389208 4.2:+80089946 MS.gene066658:CDS
ATGGGACTTTGAATACCATT+TGG 0.389462 4.2:-80090035 None:intergenic
GCCGGCAAGAGGTTGGAAAA+AGG 0.411903 4.2:-80089640 None:intergenic
TGTGAGTTAAAAGCGCTTGT+AGG 0.415532 4.2:-80090232 None:intergenic
TGTATGGTTAAAATGAAAGT+TGG 0.417382 4.2:+80090386 MS.gene066658:CDS
GGAAACCAACGTTCGATTGT+TGG 0.436462 4.2:-80090014 None:intergenic
TATGGTAATGATTTCGGATG+GGG 0.444025 4.2:+80090596 MS.gene066658:CDS
TTGGTTATCCTAAGAAAGCT+TGG 0.453190 4.2:-80090521 None:intergenic
GTTGGGGAGTTGTTGGAGGA+AGG 0.453345 4.2:+80090404 MS.gene066658:CDS
TATTAAATACTTCTTCTTGT+GGG 0.457431 4.2:-80090289 None:intergenic
GTATTTAATAGAGTCGTGAT+AGG 0.460162 4.2:+80090302 MS.gene066658:CDS
GTGGCATTTCATCAATTCAT+GGG 0.460215 4.2:+80089947 MS.gene066658:CDS
TGGATTTGGGTTTCTACCAC+CGG 0.460917 4.2:-80089585 None:intergenic
GTGGGTCAAATTGTTTGAAA+CGG 0.471551 4.2:-80090271 None:intergenic
GAGGTACGTAGACTGGTTCT+AGG 0.471874 4.2:-80089776 None:intergenic
ATCGAACGTTGGTTTCCAAA+TGG 0.475255 4.2:+80090020 MS.gene066658:CDS
AAACAATCCATTTGACTCCA+TGG 0.476645 4.2:+80089508 MS.gene066658:CDS
TGAAGAATCACTACGAGTCT+TGG 0.477110 4.2:+80090489 MS.gene066658:CDS
AAATGCAGTTACAAGATGTA+TGG 0.480196 4.2:+80090370 MS.gene066658:CDS
CTATTAAATACTTCTTCTTG+TGG 0.481064 4.2:-80090290 None:intergenic
GACACAACATCCACAAATTG+AGG 0.483588 4.2:-80090749 None:intergenic
GGGGAGTTGTTGGAGGAAGG+TGG 0.485977 4.2:+80090407 MS.gene066658:CDS
GTGAGTTAAAAGCGCTTGTA+GGG 0.487028 4.2:-80090231 None:intergenic
AATAGAGTCGTGATAGGAGT+TGG 0.491643 4.2:+80090308 MS.gene066658:CDS
CGTAGTGGATATAAAATGAA+TGG 0.496424 4.2:+80090635 MS.gene066658:CDS
TTCCGTTATTTCAAGACGGC+CGG 0.498398 4.2:-80089658 None:intergenic
TTGTATCGGTGGAAAGAACT+TGG 0.500545 4.2:-80089461 None:intergenic
CGTAAGGAAGACAGATTTGA+AGG 0.505427 4.2:-80090700 None:intergenic
TCTTTCCACCGATACAATCA+AGG 0.509277 4.2:+80089467 MS.gene066658:CDS
GAAAGTTGGGGAGTTGTTGG+AGG 0.511101 4.2:+80090400 MS.gene066658:CDS
GGAGATCCCATGGAGTCAAA+TGG 0.511534 4.2:-80089515 None:intergenic
TGCCGGCCGTCTTGAAATAA+CGG 0.512312 4.2:+80089656 MS.gene066658:CDS
GGGTCTTCTTTATCACCATC+CGG 0.514663 4.2:+80089566 MS.gene066658:CDS
CATAAAGAAAGGAAATCGTA+TGG 0.514898 4.2:-80090054 None:intergenic
TTTATGGTAATGATTTCGGA+TGG 0.520125 4.2:+80090594 MS.gene066658:CDS
GGGAAGTGACCCTCAATTTG+TGG 0.520189 4.2:+80090739 MS.gene066658:CDS
CAAGACGGCCGGCAAGAGGT+TGG 0.533473 4.2:-80089647 None:intergenic
TTAGAAAAGAATCACTCTGA+TGG 0.541983 4.2:+80090080 MS.gene066658:CDS
ACAATTGGAGGTACGTAGAC+TGG 0.544025 4.2:-80089783 None:intergenic
GAGGAAGGTGGACTTTGTAA+AGG 0.546587 4.2:+80090419 MS.gene066658:CDS
ATTTCAAGACGGCCGGCAAG+AGG 0.547420 4.2:-80089651 None:intergenic
TATCCACTACGAACTGCCAC+AGG 0.548923 4.2:-80090623 None:intergenic
AACAATCCATTTGACTCCAT+GGG 0.553051 4.2:+80089509 MS.gene066658:CDS
ATAGAGAGTGTCTCAGCCGT+TGG 0.554838 4.2:-80089605 None:intergenic
TTCAACCATGTTGTTGTCGA+CGG 0.555168 4.2:+80089915 MS.gene066658:CDS
TTATGGTAATGATTTCGGAT+GGG 0.560500 4.2:+80090595 MS.gene066658:CDS
TAGAAAAGAATCACTCTGAT+GGG 0.561164 4.2:+80090081 MS.gene066658:CDS
TGGTTGAAACAAGTAGATAA+CGG 0.562482 4.2:-80089536 None:intergenic
GTGTTTGCTGGAATAGAAGA+AGG 0.564349 4.2:+80090668 MS.gene066658:CDS
AGTAGATAACGGAGATCCCA+TGG 0.564523 4.2:-80089525 None:intergenic
TTTGTTGACTGTTCAAGTAA+CGG 0.569286 4.2:+80089860 MS.gene066658:CDS
GGTAATGGTGGAACAAACCT+TGG 0.575179 4.2:-80090332 None:intergenic
ACACAACATCCACAAATTGA+GGG 0.576915 4.2:-80090748 None:intergenic
AACCAACGTTCGATTGTTGG+AGG 0.582650 4.2:-80090011 None:intergenic
TTACCATAAACATCAAACCT+TGG 0.582755 4.2:-80090581 None:intergenic
TAGATTGTTCCACTTTACAA+AGG 0.584748 4.2:+80090148 MS.gene066658:CDS
CAAGTAACGGAGCTAGTTGA+CGG 0.586570 4.2:+80089873 MS.gene066658:CDS
TGTGTTCCGTTATTTCAAGA+CGG 0.586751 4.2:-80089662 None:intergenic
ATAAAGAAAGGAAATCGTAT+GGG 0.589028 4.2:-80090053 None:intergenic
ATTGCCTCCAAAATCTCGTA+AGG 0.590594 4.2:-80090716 None:intergenic
TTTGGGTTTCTACCACCGGA+TGG 0.595045 4.2:-80089581 None:intergenic
ATCGGTGGAAAGAACTTGGA+CGG 0.599346 4.2:-80089457 None:intergenic
TGGTGCCTTGATTGTATCGG+TGG 0.602259 4.2:-80089472 None:intergenic
TAGTCATTAGCAGTTCTCCA+AGG 0.609623 4.2:+80090564 MS.gene066658:CDS
GTTACTTGAACAGTCAACAA+AGG 0.610666 4.2:-80089858 None:intergenic
AAGCCTGTGGCAGTTCGTAG+TGG 0.624075 4.2:+80090620 MS.gene066658:CDS
AAGTATTCTCAGCTAATGCG+TGG 0.631991 4.2:-80089740 None:intergenic
TATGGTTAAAATGAAAGTTG+GGG 0.634482 4.2:+80090388 MS.gene066658:CDS
AACCTCCAACAATCGAACGT+TGG 0.637858 4.2:+80090009 MS.gene066658:CDS
TTTCGGATGGGGAAAGCCTG+TGG 0.644016 4.2:+80090607 MS.gene066658:CDS
TCGTGATAGGAGTTGGACCA+AGG 0.645786 4.2:+80090315 MS.gene066658:CDS
TGGTAGAAACCCAAATCCAA+CGG 0.650160 4.2:+80089589 MS.gene066658:CDS
TCTGTTATATGCAACAATGC+AGG 0.665996 4.2:+80089705 MS.gene066658:CDS
TGAATTGATGAAATGCCACA+AGG 0.668442 4.2:-80089943 None:intergenic
TTGTCGACGGAAAATCCTTG+TGG 0.668665 4.2:+80089928 MS.gene066658:CDS
TCTTCTTTATCACCATCCGG+TGG 0.677054 4.2:+80089569 MS.gene066658:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAATACTTCTTCTTGT+GGG - chr4.2:80090292-80090311 None:intergenic 20.0%
!!! AATGACTAAGAAATTGTTTT+TGG - chr4.2:80090552-80090571 None:intergenic 20.0%
!!! ATAGTTTTTATCTCCAATAA+AGG - chr4.2:80089823-80089842 None:intergenic 20.0%
! ATAAAGAAAGGAAATCGTAT+GGG - chr4.2:80090056-80090075 None:intergenic 25.0%
! ATTGGAGATAAAAACTATGA+AGG + chr4.2:80089825-80089844 MS.gene066658:CDS 25.0%
! CTATTAAATACTTCTTCTTG+TGG - chr4.2:80090293-80090312 None:intergenic 25.0%
! GTATGGTTAAAATGAAAGTT+GGG + chr4.2:80090387-80090406 MS.gene066658:CDS 25.0%
! TATGGTTAAAATGAAAGTTG+GGG + chr4.2:80090388-80090407 MS.gene066658:CDS 25.0%
! TCAAAAATCAACAAAGAAGA+CGG + chr4.2:80090191-80090210 MS.gene066658:CDS 25.0%
! TGTATGGTTAAAATGAAAGT+TGG + chr4.2:80090386-80090405 MS.gene066658:CDS 25.0%
!! ATTGTTTGATCACTTGATTT+TGG - chr4.2:80089495-80089514 None:intergenic 25.0%
!! CCATTACCAGAAAATTATTT+TGG + chr4.2:80090347-80090366 MS.gene066658:CDS 25.0%
!! GATGTTTATGGTAATGATTT+CGG + chr4.2:80090590-80090609 MS.gene066658:CDS 25.0%
!! GCATTTCCAAAATAATTTTC+TGG - chr4.2:80090356-80090375 None:intergenic 25.0%
!! TTTTCTAACTCCATAAAGAA+AGG - chr4.2:80090068-80090087 None:intergenic 25.0%
!!! AAATAATTTTCTGGTAATGG+TGG - chr4.2:80090347-80090366 None:intergenic 25.0%
!!! CCAAAATAATTTTCTGGTAA+TGG - chr4.2:80090350-80090369 None:intergenic 25.0%
AAAAATGAATCGACAATTGG+AGG - chr4.2:80089798-80089817 None:intergenic 30.0%
AAATGCAGTTACAAGATGTA+TGG + chr4.2:80090370-80090389 MS.gene066658:CDS 30.0%
ACTTGTTTCAACCAACAAAA+AGG + chr4.2:80089545-80089564 MS.gene066658:CDS 30.0%
CATAAAGAAAGGAAATCGTA+TGG - chr4.2:80090057-80090076 None:intergenic 30.0%
CATACGATTTCCTTTCTTTA+TGG + chr4.2:80090055-80090074 MS.gene066658:CDS 30.0%
CTTGTTTCAACCAACAAAAA+GGG + chr4.2:80089546-80089565 MS.gene066658:CDS 30.0%
GTATTTAATAGAGTCGTGAT+AGG + chr4.2:80090302-80090321 MS.gene066658:CDS 30.0%
TAAGAAATCCAAGCTTTCTT+AGG + chr4.2:80090513-80090532 MS.gene066658:CDS 30.0%
TAGAAAAGAATCACTCTGAT+GGG + chr4.2:80090081-80090100 MS.gene066658:CDS 30.0%
TAGATTGTTCCACTTTACAA+AGG + chr4.2:80090148-80090167 MS.gene066658:CDS 30.0%
TGGTTGAAACAAGTAGATAA+CGG - chr4.2:80089539-80089558 None:intergenic 30.0%
TTACCATAAACATCAAACCT+TGG - chr4.2:80090584-80090603 None:intergenic 30.0%
TTAGAAAAGAATCACTCTGA+TGG + chr4.2:80090080-80090099 MS.gene066658:CDS 30.0%
! ATAAAGAAGACCCTTTTTGT+TGG - chr4.2:80089559-80089578 None:intergenic 30.0%
! GTGATTCTTCAACTTTTCAT+CGG - chr4.2:80090480-80090499 None:intergenic 30.0%
! TTATGGTAATGATTTCGGAT+GGG + chr4.2:80090595-80090614 MS.gene066658:CDS 30.0%
! TTTATGGTAATGATTTCGGA+TGG + chr4.2:80090594-80090613 MS.gene066658:CDS 30.0%
! TTTGTTGACTGTTCAAGTAA+CGG + chr4.2:80089860-80089879 MS.gene066658:CDS 30.0%
!! CGTAGTGGATATAAAATGAA+TGG + chr4.2:80090635-80090654 MS.gene066658:CDS 30.0%
!! GATTCATTTTTCGCCTTTAT+TGG + chr4.2:80089807-80089826 MS.gene066658:CDS 30.0%
!! TTGTAGGGAAGATATTTTGA+TGG - chr4.2:80090219-80090238 None:intergenic 30.0%
!!! GAAATTGTTTTTGGCTATGT+TGG - chr4.2:80090543-80090562 None:intergenic 30.0%
AAACAATCCATTTGACTCCA+TGG + chr4.2:80089508-80089527 MS.gene066658:CDS 35.0%
AACAATCCATTTGACTCCAT+GGG + chr4.2:80089509-80089528 MS.gene066658:CDS 35.0%
AAGAGGTTGGAAAAAGGAAA+GGG - chr4.2:80089637-80089656 None:intergenic 35.0%
ACACAACATCCACAAATTGA+GGG - chr4.2:80090751-80090770 None:intergenic 35.0%
ATGGGACTTTGAATACCATT+TGG - chr4.2:80090038-80090057 None:intergenic 35.0%
CTAGTTGACGGTATCTTTAT+TGG + chr4.2:80089885-80089904 MS.gene066658:CDS 35.0%
GCGAAAAATGAATCGACAAT+TGG - chr4.2:80089801-80089820 None:intergenic 35.0%
GTGGCATTTCATCAATTCAT+GGG + chr4.2:80089947-80089966 MS.gene066658:CDS 35.0%
GTGGGTCAAATTGTTTGAAA+CGG - chr4.2:80090274-80090293 None:intergenic 35.0%
GTTACTTGAACAGTCAACAA+AGG - chr4.2:80089861-80089880 None:intergenic 35.0%
TCTCCAAGGTTTGATGTTTA+TGG + chr4.2:80090578-80090597 MS.gene066658:CDS 35.0%
TCTGTTATATGCAACAATGC+AGG + chr4.2:80089705-80089724 MS.gene066658:CDS 35.0%
TGAATTGATGAAATGCCACA+AGG - chr4.2:80089946-80089965 None:intergenic 35.0%
TGTGGCATTTCATCAATTCA+TGG + chr4.2:80089946-80089965 MS.gene066658:CDS 35.0%
TTGGTTATCCTAAGAAAGCT+TGG - chr4.2:80090524-80090543 None:intergenic 35.0%
! CGATTTTCTCCTTTGTAAAG+TGG - chr4.2:80090160-80090179 None:intergenic 35.0%
! TATGGTAATGATTTCGGATG+GGG + chr4.2:80090596-80090615 MS.gene066658:CDS 35.0%
! TGTGTTCCGTTATTTCAAGA+CGG - chr4.2:80089665-80089684 None:intergenic 35.0%
!! AAGCGCTTTTAACTCACATT+TGG + chr4.2:80090237-80090256 MS.gene066658:CDS 35.0%
!!! TTTTGGTGCCTTGATTGTAT+CGG - chr4.2:80089478-80089497 None:intergenic 35.0%
AAGTATTCTCAGCTAATGCG+TGG - chr4.2:80089743-80089762 None:intergenic 40.0%
AATAGAGTCGTGATAGGAGT+TGG + chr4.2:80090308-80090327 MS.gene066658:CDS 40.0%
AATGAAAGTTGGGGAGTTGT+TGG + chr4.2:80090397-80090416 MS.gene066658:CDS 40.0%
ATTGCCTCCAAAATCTCGTA+AGG - chr4.2:80090719-80090738 None:intergenic 40.0%
CAAGAGGTTGGAAAAAGGAA+AGG - chr4.2:80089638-80089657 None:intergenic 40.0%
CGTAAGGAAGACAGATTTGA+AGG - chr4.2:80090703-80090722 None:intergenic 40.0%
GACACAACATCCACAAATTG+AGG - chr4.2:80090752-80090771 None:intergenic 40.0%
TAGTCATTAGCAGTTCTCCA+AGG + chr4.2:80090564-80090583 MS.gene066658:CDS 40.0%
TCTTTCCACCGATACAATCA+AGG + chr4.2:80089467-80089486 MS.gene066658:CDS 40.0%
TGAAGAATCACTACGAGTCT+TGG + chr4.2:80090489-80090508 MS.gene066658:CDS 40.0%
TGGTAGAAACCCAAATCCAA+CGG + chr4.2:80089589-80089608 MS.gene066658:CDS 40.0%
TTCAACCATGTTGTTGTCGA+CGG + chr4.2:80089915-80089934 MS.gene066658:CDS 40.0%
! ATTTTCCGTCGACAACAACA+TGG - chr4.2:80089923-80089942 None:intergenic 40.0%
! CTGTCTTCCTTACGAGATTT+TGG + chr4.2:80090709-80090728 MS.gene066658:CDS 40.0%
! GTGTTTGCTGGAATAGAAGA+AGG + chr4.2:80090668-80090687 MS.gene066658:CDS 40.0%
! TCTTCCTTACGAGATTTTGG+AGG + chr4.2:80090712-80090731 MS.gene066658:CDS 40.0%
! TTGTATCGGTGGAAAGAACT+TGG - chr4.2:80089464-80089483 None:intergenic 40.0%
!! ATCGAACGTTGGTTTCCAAA+TGG + chr4.2:80090020-80090039 MS.gene066658:CDS 40.0%
!! GGACTTGTTAATGTGTTTGC+TGG + chr4.2:80090656-80090675 MS.gene066658:CDS 40.0%
!! GTGAGTTAAAAGCGCTTGTA+GGG - chr4.2:80090234-80090253 None:intergenic 40.0%
!! TACGAGATTTTGGAGGCAAT+GGG + chr4.2:80090719-80090738 MS.gene066658:CDS 40.0%
!! TGTGAGTTAAAAGCGCTTGT+AGG - chr4.2:80090235-80090254 None:intergenic 40.0%
!! TTACGAGATTTTGGAGGCAA+TGG + chr4.2:80090718-80090737 MS.gene066658:CDS 40.0%
AACCAACGTTCGATTGTTGG+AGG - chr4.2:80090014-80090033 None:intergenic 45.0%
AACCTCCAACAATCGAACGT+TGG + chr4.2:80090009-80090028 MS.gene066658:CDS 45.0%
ATCGGTGGAAAGAACTTGGA+CGG - chr4.2:80089460-80089479 None:intergenic 45.0%
GGAAACCAACGTTCGATTGT+TGG - chr4.2:80090017-80090036 None:intergenic 45.0%
GGGTCTTCTTTATCACCATC+CGG + chr4.2:80089566-80089585 MS.gene066658:CDS 45.0%
TCTTCTTTATCACCATCCGG+TGG + chr4.2:80089569-80089588 MS.gene066658:CDS 45.0%
TTGGAAAAAGGAAAGGGCAG+AGG - chr4.2:80089631-80089650 None:intergenic 45.0%
TTGTCGACGGAAAATCCTTG+TGG + chr4.2:80089928-80089947 MS.gene066658:CDS 45.0%
! ACAATTGGAGGTACGTAGAC+TGG - chr4.2:80089786-80089805 None:intergenic 45.0%
! CAAGTAACGGAGCTAGTTGA+CGG + chr4.2:80089873-80089892 MS.gene066658:CDS 45.0%
! GAGGAAGGTGGACTTTGTAA+AGG + chr4.2:80090419-80090438 MS.gene066658:CDS 45.0%
! GGTAATGGTGGAACAAACCT+TGG - chr4.2:80090335-80090354 None:intergenic 45.0%
! TCCTTTTTCCAACCTCTTGC+CGG + chr4.2:80089639-80089658 MS.gene066658:CDS 45.0%
! TGGATTTGGGTTTCTACCAC+CGG - chr4.2:80089588-80089607 None:intergenic 45.0%
! TTCCGTTATTTCAAGACGGC+CGG - chr4.2:80089661-80089680 None:intergenic 45.0%
!! AGTAGATAACGGAGATCCCA+TGG - chr4.2:80089528-80089547 None:intergenic 45.0%
AGTGTCTCAGCCGTTGGATT+TGG - chr4.2:80089602-80089621 None:intergenic 50.0%
GAAAGTTGGGGAGTTGTTGG+AGG + chr4.2:80090400-80090419 MS.gene066658:CDS 50.0%
GGAGATCCCATGGAGTCAAA+TGG - chr4.2:80089518-80089537 None:intergenic 50.0%
GGGAAGTGACCCTCAATTTG+TGG + chr4.2:80090739-80090758 MS.gene066658:CDS 50.0%
GTGTCTCAGCCGTTGGATTT+GGG - chr4.2:80089601-80089620 None:intergenic 50.0%
TATCCACTACGAACTGCCAC+AGG - chr4.2:80090626-80090645 None:intergenic 50.0%
TCGTGATAGGAGTTGGACCA+AGG + chr4.2:80090315-80090334 MS.gene066658:CDS 50.0%
TGCCGGCCGTCTTGAAATAA+CGG + chr4.2:80089656-80089675 MS.gene066658:CDS 50.0%
TGGTGCCTTGATTGTATCGG+TGG - chr4.2:80089475-80089494 None:intergenic 50.0%
! GAGGTACGTAGACTGGTTCT+AGG - chr4.2:80089779-80089798 None:intergenic 50.0%
! TTTGGGTTTCTACCACCGGA+TGG - chr4.2:80089584-80089603 None:intergenic 50.0%
!! ATAGAGAGTGTCTCAGCCGT+TGG - chr4.2:80089608-80089627 None:intergenic 50.0%
AAGCCTGTGGCAGTTCGTAG+TGG + chr4.2:80090620-80090639 MS.gene066658:CDS 55.0%
ATTTCAAGACGGCCGGCAAG+AGG - chr4.2:80089654-80089673 None:intergenic 55.0%
GCCGGCAAGAGGTTGGAAAA+AGG - chr4.2:80089643-80089662 None:intergenic 55.0%
GTTGGGGAGTTGTTGGAGGA+AGG + chr4.2:80090404-80090423 MS.gene066658:CDS 55.0%
TTTCGGATGGGGAAAGCCTG+TGG + chr4.2:80090607-80090626 MS.gene066658:CDS 55.0%
GGGGAGTTGTTGGAGGAAGG+TGG + chr4.2:80090407-80090426 MS.gene066658:CDS 60.0%
CAAGACGGCCGGCAAGAGGT+TGG - chr4.2:80089650-80089669 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 80089450 80090778 80089450 ID=MS.gene066658
chr4.2 mRNA 80089450 80090778 80089450 ID=MS.gene066658.t1;Parent=MS.gene066658
chr4.2 exon 80089450 80090778 80089450 ID=MS.gene066658.t1.exon1;Parent=MS.gene066658.t1
chr4.2 CDS 80089450 80090778 80089450 ID=cds.MS.gene066658.t1;Parent=MS.gene066658.t1
Gene Sequence

>MS.gene066658

ATGGGTGCCGTCCAAGTTCTTTCCACCGATACAATCAAGGCACCAAAATCAAGTGATCAAACAATCCATTTGACTCCATGGGATCTCCGTTATCTACTTGTTTCAACCAACAAAAAGGGTCTTCTTTATCACCATCCGGTGGTAGAAACCCAAATCCAACGGCTGAGACACTCTCTATCCTCTGCCCTTTCCTTTTTCCAACCTCTTGCCGGCCGTCTTGAAATAACGGAACACAAAGACAATATTGTCTCGTGTTCTGTTATATGCAACAATGCAGGTGTACTCTTTATCCACGCATTAGCTGAGAATACTTGTGTTGCTGACATCCTAGAACCAGTCTACGTACCTCCAATTGTCGATTCATTTTTCGCCTTTATTGGAGATAAAAACTATGAAGGCACGTCAAAACCTTTGTTGACTGTTCAAGTAACGGAGCTAGTTGACGGTATCTTTATTGGCTGCACATTCAACCATGTTGTTGTCGACGGAAAATCCTTGTGGCATTTCATCAATTCATGGGCTGAAATCTCACGTAATTGTTGTCATCATCAAATATCTAAACCTCCAACAATCGAACGTTGGTTTCCAAATGGTATTCAAAGTCCCATACGATTTCCTTTCTTTATGGAGTTAGAAAAGAATCACTCTGATGGGTTGACTATTTCATCATCTAATGATGAAAAACTTTGTTTATTAAATAGATTGTTCCACTTTACAAAGGAGAAAATCGTGCAACTTAAATCAAAAATCAACAAAGAAGACGGTACCATCAAAATATCTTCCCTACAAGCGCTTTTAACTCACATTTGGTGTTATGTTATCCGTTTCAAACAATTTGACCCACAAGAAGAAGTATTTAATAGAGTCGTGATAGGAGTTGGACCAAGGTTTGTTCCACCATTACCAGAAAATTATTTTGGAAATGCAGTTACAAGATGTATGGTTAAAATGAAAGTTGGGGAGTTGTTGGAGGAAGGTGGACTTTGTAAAGGTGCTTGTGAGATGAACAAGTTAATTGCTTCACACACCGATGAAAAGTTGAAGAATCACTACGAGTCTTGGTTAAGAAATCCAAGCTTTCTTAGGATAACCAACATAGCCAAAAACAATTTCTTAGTCATTAGCAGTTCTCCAAGGTTTGATGTTTATGGTAATGATTTCGGATGGGGAAAGCCTGTGGCAGTTCGTAGTGGATATAAAATGAATGGACTTGTTAATGTGTTTGCTGGAATAGAAGAAGGTAGCATTGACCTTCAAATCTGTCTTCCTTACGAGATTTTGGAGGCAATGGGAAGTGACCCTCAATTTGTGGATGTTGTGTCCAACTAA
Protein sequence

>MS.gene066658.t1

MGAVQVLSTDTIKAPKSSDQTIHLTPWDLRYLLVSTNKKGLLYHHPVVETQIQRLRHSLSSALSFFQPLAGRLEITEHKDNIVSCSVICNNAGVLFIHALAENTCVADILEPVYVPPIVDSFFAFIGDKNYEGTSKPLLTVQVTELVDGIFIGCTFNHVVVDGKSLWHFINSWAEISRNCCHHQISKPPTIERWFPNGIQSPIRFPFFMELEKNHSDGLTISSSNDEKLCLLNRLFHFTKEKIVQLKSKINKEDGTIKISSLQALLTHIWCYVIRFKQFDPQEEVFNRVVIGVGPRFVPPLPENYFGNAVTRCMVKMKVGELLEEGGLCKGACEMNKLIASHTDEKLKNHYESWLRNPSFLRITNIAKNNFLVISSSPRFDVYGNDFGWGKPVAVRSGYKMNGLVNVFAGIEEGSIDLQICLPYEILEAMGSDPQFVDVVSN