AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87191

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87191.t1 MTR_3g088420 61.785 437 157 5 1 436 1 428 0.0 541
MS.gene87191.t1 MTR_4g022825 60.802 449 158 7 1 440 1 440 0.0 531
MS.gene87191.t1 MTR_8g041770 59.318 440 171 6 1 436 1 436 0.0 517
MS.gene87191.t1 MTR_0190s0020 58.864 440 174 6 1 436 1 437 1.35e-179 509
MS.gene87191.t1 MTR_4g022720 58.447 438 172 5 1 436 1 430 7.87e-175 497
MS.gene87191.t1 MTR_3g026310 58.575 449 163 8 1 434 1 441 5.17e-173 493
MS.gene87191.t1 MTR_4g022800 57.556 450 172 9 1 441 1 440 2.41e-171 489
MS.gene87191.t1 MTR_4g022670 56.982 444 175 6 1 436 1 436 1.13e-168 482
MS.gene87191.t1 MTR_7g026860 56.629 445 172 8 1 436 1 433 9.20e-166 474
MS.gene87191.t1 MTR_8g040780 90.361 249 21 2 213 458 1 249 1.38e-161 456
MS.gene87191.t1 MTR_4g094842 52.466 446 199 7 1 436 1 443 7.03e-157 452
MS.gene87191.t1 MTR_4g094835 51.535 456 199 10 1 443 1 447 7.55e-153 442
MS.gene87191.t1 MTR_4g022760 51.351 444 156 6 1 436 1 392 1.18e-144 419
MS.gene87191.t1 MTR_8g040760 89.671 213 22 0 1 213 1 213 1.74e-140 401
MS.gene87191.t1 MTR_4g094838 48.775 449 192 8 4 436 1 427 9.69e-140 408
MS.gene87191.t1 MTR_8g079710 48.879 446 204 12 1 432 1 436 2.60e-133 392
MS.gene87191.t1 MTR_4g094832 55.645 372 146 7 78 436 60 425 4.46e-133 391
MS.gene87191.t1 MTR_4g094828 47.884 449 211 12 1 433 1 442 3.76e-131 387
MS.gene87191.t1 MTR_4g022290 47.894 451 205 12 1 434 1 438 7.62e-130 383
MS.gene87191.t1 MTR_6g016815 45.805 441 163 9 1 441 15 379 9.77e-116 345
MS.gene87191.t1 MTR_3g101970 35.920 451 256 13 3 439 5 436 5.33e-77 247
MS.gene87191.t1 MTR_3g101900 36.449 428 246 11 21 433 24 440 6.74e-77 247
MS.gene87191.t1 MTR_3g102020 36.384 437 255 11 15 436 18 446 9.53e-74 239
MS.gene87191.t1 MTR_3g101960 34.956 452 269 13 3 438 5 447 3.09e-73 238
MS.gene87191.t1 MTR_3g102010 35.202 446 270 12 4 436 7 446 6.92e-71 232
MS.gene87191.t1 MTR_3g101930 35.211 426 255 8 27 436 30 450 1.35e-68 226
MS.gene87191.t1 MTR_7g026850 57.595 158 64 1 32 189 12 166 9.50e-57 186
MS.gene87191.t1 MTR_4g015180 31.656 477 252 14 20 436 20 482 2.67e-53 186
MS.gene87191.t1 MTR_5g081960 28.059 474 262 11 25 436 25 481 2.75e-52 183
MS.gene87191.t1 MTR_3g070170 29.536 474 277 14 2 436 10 465 6.63e-52 182
MS.gene87191.t1 MTR_4g094825 47.959 196 94 4 13 204 78 269 6.19e-50 175
MS.gene87191.t1 MTR_4g094825 52.055 146 59 1 291 436 252 386 6.82e-42 153
MS.gene87191.t1 MTR_7g026770 50.262 191 71 3 148 336 133 301 1.30e-44 158
MS.gene87191.t1 MTR_6g034485 57.937 126 48 3 194 317 2 124 1.57e-42 147
MS.gene87191.t1 MTR_6g034495 63.248 117 39 2 78 190 51 167 4.65e-42 147
MS.gene87191.t1 MTR_6g045553 28.540 452 279 12 22 438 30 472 1.03e-39 149
MS.gene87191.t1 MTR_3g102030 31.045 335 189 10 72 395 17 320 3.18e-39 144
MS.gene87191.t1 MTR_7g026830 63.095 84 31 0 88 171 14 97 1.14e-30 114
MS.gene87191.t1 MTR_2g105330 27.068 399 256 17 46 423 34 418 2.34e-27 114
MS.gene87191.t1 MTR_2g064495 26.980 404 250 16 46 423 34 418 7.23e-27 112
MS.gene87191.t1 MTR_4g007540 25.581 387 259 15 61 432 56 428 6.63e-26 109
MS.gene87191.t1 MTR_8g075610 26.961 408 254 18 47 430 26 413 2.13e-24 105
MS.gene87191.t1 MTR_7g026800 37.427 171 72 4 223 386 31 173 2.00e-23 97.8
MS.gene87191.t1 MTR_8g096530 27.457 346 217 12 59 390 57 382 1.67e-22 100
MS.gene87191.t1 MTR_4g022780 35.294 170 76 3 223 386 32 173 3.11e-22 94.0
MS.gene87191.t1 MTR_3g098980 25.269 372 247 13 62 422 67 418 9.40e-21 94.7
MS.gene87191.t1 MTR_7g026780 46.667 105 30 2 330 434 26 104 3.16e-20 86.3
MS.gene87191.t1 MTR_3g026950 25.783 415 260 16 45 426 38 437 5.91e-20 92.4
MS.gene87191.t1 MTR_4g055170 27.632 304 186 14 62 349 70 355 9.30e-19 89.0
MS.gene87191.t1 MTR_3g049150 24.627 402 257 15 58 430 52 436 4.38e-18 86.7
MS.gene87191.t1 MTR_4g097090 23.908 435 274 17 24 422 27 440 7.25e-18 86.3
MS.gene87191.t1 MTR_3g112180 24.760 416 268 17 31 428 38 426 1.69e-16 82.0
MS.gene87191.t1 MTR_1g081660 22.350 434 263 18 45 443 57 451 1.55e-15 79.0
MS.gene87191.t1 MTR_3g071320 21.538 390 261 13 25 391 24 391 4.32e-15 77.4
MS.gene87191.t1 MTR_7g085990 23.671 414 254 10 53 417 47 447 1.35e-14 76.3
MS.gene87191.t1 MTR_7g026870 52.564 78 36 1 273 349 74 151 1.53e-14 71.2
MS.gene87191.t1 MTR_2g089755 23.373 415 253 16 27 394 37 433 2.26e-14 75.5
MS.gene87191.t1 MTR_7g085930 24.079 407 261 10 53 417 51 451 4.26e-14 74.7
MS.gene87191.t1 MTR_3g465990 24.224 322 205 14 41 344 45 345 5.22e-14 74.3
MS.gene87191.t1 MTR_7g084940 26.354 277 180 9 59 322 67 332 6.42e-14 73.9
MS.gene87191.t1 MTR_4g013050 22.969 357 207 14 59 391 51 363 1.36e-13 72.8
MS.gene87191.t1 MTR_1g053315 24.808 391 240 20 63 423 80 446 1.52e-13 72.8
MS.gene87191.t1 MTR_5g076700 23.134 402 265 18 44 423 48 427 1.59e-13 72.8
MS.gene87191.t1 MTR_5g026320 22.970 431 275 18 44 446 57 458 4.65e-13 71.2
MS.gene87191.t1 MTR_6g015320 24.551 167 125 1 234 400 251 416 5.74e-13 70.9
MS.gene87191.t1 MTR_3g049140 25.630 238 150 9 210 430 6 233 1.01e-12 68.6
MS.gene87191.t1 MTR_6g015815 26.404 178 124 3 232 407 256 428 2.06e-12 69.3
MS.gene87191.t1 MTR_6g015315 22.500 320 218 10 135 431 133 445 3.76e-12 68.6
MS.gene87191.t1 MTR_3g086020 22.148 447 295 15 5 439 6 411 6.73e-12 67.4
MS.gene87191.t1 MTR_0502s0010 28.151 238 143 11 101 323 1 225 7.57e-12 65.5
MS.gene87191.t1 MTR_8g079980 21.410 383 247 15 44 403 42 393 8.07e-12 67.4
MS.gene87191.t1 MTR_3g465910 24.918 305 191 12 56 344 63 345 2.05e-11 66.2
MS.gene87191.t1 MTR_3g465930 25.246 305 182 13 61 344 69 348 2.15e-11 66.2
MS.gene87191.t1 MTR_7g084910 25.090 279 182 8 59 324 68 332 2.79e-11 65.5
MS.gene87191.t1 MTR_7g026880 55.102 49 20 1 388 436 2 48 3.14e-11 58.9
MS.gene87191.t1 MTR_7g014200 21.445 443 286 13 25 417 28 458 5.50e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87191.t1 AT5G67150 37.387 444 256 13 6 436 7 441 1.68e-85 269
MS.gene87191.t1 AT3G50270 37.647 425 254 9 6 422 8 429 4.00e-81 258
MS.gene87191.t1 AT3G50300 36.364 451 263 12 1 440 1 438 6.73e-77 247
MS.gene87191.t1 AT3G50280 34.004 447 275 10 1 436 1 438 4.01e-75 242
MS.gene87191.t1 AT5G42830 32.414 435 279 9 12 436 15 444 2.93e-74 240
MS.gene87191.t1 AT5G38130 35.927 437 250 11 1 422 21 442 6.10e-73 237
MS.gene87191.t1 AT5G67160 35.828 441 250 13 6 437 12 428 2.78e-71 232
MS.gene87191.t1 AT5G07850 33.187 455 286 10 2 443 6 455 1.00e-68 226
MS.gene87191.t1 AT5G07860 33.038 451 274 12 2 436 6 444 1.15e-67 223
MS.gene87191.t1 AT2G39980 32.196 469 251 12 20 436 22 475 4.53e-64 214
MS.gene87191.t1 AT5G07870 32.397 463 288 12 2 443 6 464 4.63e-63 211
MS.gene87191.t1 AT5G01210 31.291 457 262 12 20 436 20 464 2.18e-58 199
MS.gene87191.t1 AT5G23940 30.283 459 278 17 5 436 4 447 3.95e-52 183
MS.gene87191.t1 AT3G50280 34.824 313 192 6 131 436 1 308 4.83e-51 176
MS.gene87191.t1 AT3G50290 37.729 273 151 9 128 388 4 269 5.09e-41 147
MS.gene87191.t1 AT5G48930 26.667 390 261 13 46 422 41 418 4.24e-28 116
MS.gene87191.t1 AT3G48720 27.543 403 251 16 45 429 44 423 1.82e-25 108
MS.gene87191.t1 AT5G41040 25.964 389 250 14 58 429 82 449 1.31e-23 103
MS.gene87191.t1 AT5G41040 25.888 394 244 15 58 429 66 433 1.31e-23 103
MS.gene87191.t1 AT2G40230 25.967 362 247 11 44 395 41 391 1.92e-22 99.8
MS.gene87191.t1 AT5G63560 26.456 412 245 18 42 429 42 419 1.82e-18 87.8
MS.gene87191.t1 AT5G57840 24.205 409 258 16 43 422 37 422 1.36e-16 82.0
MS.gene87191.t1 AT2G19070 22.912 419 275 16 44 430 41 443 7.00e-16 80.1
MS.gene87191.t1 AT3G03480 26.024 415 244 20 45 428 57 439 2.14e-15 78.6
MS.gene87191.t1 AT5G17540 23.973 438 266 18 28 428 23 430 2.18e-15 78.6
MS.gene87191.t1 AT3G29590 22.149 456 311 16 2 432 6 442 1.53e-14 75.9
MS.gene87191.t1 AT1G27620 24.928 349 238 13 61 399 58 392 8.10e-14 73.6
MS.gene87191.t1 AT3G29670 21.729 428 286 14 24 422 26 433 1.31e-13 73.2
MS.gene87191.t1 AT1G28680 24.542 273 171 12 138 393 135 389 3.08e-13 72.0
MS.gene87191.t1 AT1G65450 24.028 283 186 12 59 323 70 341 1.01e-12 70.1
MS.gene87191.t1 AT3G30280 21.134 388 265 14 23 393 25 388 2.35e-12 68.9
MS.gene87191.t1 AT5G07080 25.362 276 176 12 61 320 70 331 3.85e-12 68.6
MS.gene87191.t1 AT1G24430 22.137 393 261 15 34 415 40 398 1.96e-11 66.2
MS.gene87191.t1 AT5G61160 21.659 434 294 16 25 429 23 439 2.83e-11 65.9
MS.gene87191.t1 AT5G16410 24.500 400 252 18 14 391 48 419 5.15e-11 65.1
MS.gene87191.t1 AT1G78990 23.836 365 243 14 44 391 50 396 9.77e-11 63.9

Find 115 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATATTCTCCTTTGTAAAA+TGG 0.189048 8.4:+58407813 None:intergenic
GTGTCTTCCATACAAGATTT+TGG 0.229588 8.4:-58407258 MS.gene87191:CDS
CAATTGTCATATGATTATTT+AGG 0.234653 8.4:-58407626 MS.gene87191:CDS
TAATAGCTCCTTGACTTTCA+TGG 0.273620 8.4:+58407577 None:intergenic
AGTATTTACAATATTGATAT+TGG 0.302617 8.4:-58407383 MS.gene87191:CDS
TTCTTGTGGGCCAAGTTGTT+TGG 0.303580 8.4:+58407694 None:intergenic
TCTCTCTACCACTACTATTA+AGG 0.338968 8.4:-58408500 MS.gene87191:CDS
TTCGCATTTCATCAATTCAT+TGG 0.349634 8.4:-58408002 MS.gene87191:CDS
CTATCAGCCTTAACAAAGTT+TGG 0.349682 8.4:+58407452 None:intergenic
TACCTTCGTAGTTTATAATT+CGG 0.356699 8.4:+58408122 None:intergenic
GGTGATAAAGAAACCCCTTT+TGG 0.367473 8.4:+58408398 None:intergenic
TTACAATATTGATATTGGTT+GGG 0.374062 8.4:-58407378 MS.gene87191:CDS
AAGCGCTTTGCACCCACCTT+TGG 0.380154 8.4:-58407733 MS.gene87191:CDS
TACAATATTGATATTGGTTG+GGG 0.385917 8.4:-58407377 MS.gene87191:CDS
ATGAACCATGTTGTCATTGA+TGG 0.388037 8.4:-58408034 MS.gene87191:CDS
AAAGAGACTGTTTAAAGTGT+TGG 0.388856 8.4:+58408344 None:intergenic
GATCTTCAGTTCCTTCCGTT+TGG 0.390790 8.4:-58408427 MS.gene87191:CDS
TATTGTAAATACTGAACAAT+GGG 0.393542 8.4:+58407393 None:intergenic
TGAATTGATGAAATGCGAAT+TGG 0.396696 8.4:+58408006 None:intergenic
TTTACAATGCAGTCGGTTGT+AGG 0.406712 8.4:-58407162 None:intergenic
TTTACAATATTGATATTGGT+TGG 0.411960 8.4:-58407379 MS.gene87191:CDS
AAATGGAATATTCTCTCAGT+TGG 0.431761 8.4:+58407830 None:intergenic
CGTCAACTAACTCTGTTACT+TGG 0.435188 8.4:+58408077 None:intergenic
TTGGAGGTACAGATATTTGA+TGG 0.438975 8.4:+58407957 None:intergenic
AACACAAAATTCACTTCTTG+TGG 0.439166 8.4:+58407680 None:intergenic
TTAGTTGAGTCACCTACATT+TGG 0.439647 8.4:+58408475 None:intergenic
CCGTTTGGATCCAACCAAAA+GGG 0.441426 8.4:-58408412 MS.gene87191:CDS
TAAACAGTCAATATGAATCT+TGG 0.441881 8.4:-58407484 MS.gene87191:CDS
ACATGTGTTGCCGACATCCT+TGG 0.445731 8.4:-58408190 MS.gene87191:CDS
ATGACCTCCAAAATCTTGTA+TGG 0.449025 8.4:+58407251 None:intergenic
TAGTTTATAATTCGGTTAAG+TGG 0.449322 8.4:+58408130 None:intergenic
TGGTAAAGAAGAAGGAAGTA+TGG 0.453284 8.4:-58407291 MS.gene87191:CDS
GAAATGCTATGATAGTTTGC+GGG 0.453745 8.4:-58407604 MS.gene87191:CDS
CTATTAGAAGAAGGTGGGCT+TGG 0.454702 8.4:-58407560 MS.gene87191:CDS
CTTGAACGCTTATTTCCAAA+CGG 0.462676 8.4:-58407932 MS.gene87191:CDS
ATGGGGCGCTGAATTCCGTT+TGG 0.464989 8.4:+58407917 None:intergenic
GATGACCAAGGAATTACTAT+TGG 0.466288 8.4:+58407424 None:intergenic
AAGAACACCAAACTTTGTTA+AGG 0.466587 8.4:-58407459 MS.gene87191:CDS
AATTCGGTTAAGTGGAAAGA+AGG 0.467303 8.4:+58408138 None:intergenic
TCCGTTTGGATCCAACCAAA+AGG 0.468186 8.4:-58408413 MS.gene87191:CDS
GGAAATAAGCGTTCAAGTGT+TGG 0.472403 8.4:+58407938 None:intergenic
GGAAAGATTAGTGTGTTTGC+TGG 0.473290 8.4:-58407311 MS.gene87191:CDS
GTTGGTACCGGCCTCTACAT+TGG 0.477478 8.4:+58407772 None:intergenic
ACATGATCGATGCATCTACT+TGG 0.480123 8.4:-58407208 MS.gene87191:CDS
TCAGAACGGATCTTAATTTG+TGG 0.481803 8.4:+58407869 None:intergenic
TATGTATAAGTGTTAGATCA+AGG 0.487960 8.4:-58407658 MS.gene87191:CDS
TATTATGAAAGGAAATCGTA+TGG 0.495954 8.4:+58407898 None:intergenic
GGTAGTTCAAATAAAAGTAA+TGG 0.496789 8.4:-58407332 MS.gene87191:CDS
GAAGAAGGTGGGCTTGGTAA+AGG 0.502798 8.4:-58407554 MS.gene87191:CDS
GGAAATGCTATGATAGTTTG+CGG 0.503669 8.4:-58407605 MS.gene87191:CDS
AACCGACTGCATTGTAAAGA+AGG 0.508715 8.4:+58407167 None:intergenic
ACACAAAATTCACTTCTTGT+GGG 0.512772 8.4:+58407681 None:intergenic
AATGGAATATTCTCTCAGTT+GGG 0.513206 8.4:+58407831 None:intergenic
AGGAGTGGAAAACAGAAGGA+AGG 0.515796 8.4:+58408158 None:intergenic
CAAGTAACAGAGTTAGTTGA+CGG 0.521968 8.4:-58408076 MS.gene87191:CDS
TATAAGTGTTAGATCAAGGT+TGG 0.526724 8.4:-58407654 MS.gene87191:CDS
GTGTTTGCTGGTAAAGAAGA+AGG 0.526788 8.4:-58407299 MS.gene87191:CDS
TGTGGCCAATAGTAATTCCT+TGG 0.532331 8.4:-58407429 MS.gene87191:CDS
GTGGGCCAAGTTGTTTGGAA+CGG 0.534920 8.4:+58407699 None:intergenic
GTGGGTGCAAAGCGCTTGCA+TGG 0.537574 8.4:+58407739 None:intergenic
ATTATGAAAGGAAATCGTAT+GGG 0.537701 8.4:+58407899 None:intergenic
ATTTCGAGACGACATGCAAA+GGG 0.543479 8.4:+58408298 None:intergenic
GCATCGATCATGTTTAACTC+AGG 0.544730 8.4:+58407218 None:intergenic
AAGAAGGAGTGGAAAACAGA+AGG 0.551644 8.4:+58408154 None:intergenic
ACTATTAAGGCACCAAATGT+AGG 0.552094 8.4:-58408487 MS.gene87191:CDS
AACGGATCACTGAGCGCCAA+AGG 0.552464 8.4:+58407717 None:intergenic
GTCAAGGAGCTATTAGAAGA+AGG 0.552540 8.4:-58407569 MS.gene87191:CDS
CTGCATTGTAAAGAAGGTTG+TGG 0.554258 8.4:+58407173 None:intergenic
CGTTTGGATCCAACCAAAAG+GGG 0.556151 8.4:-58408411 MS.gene87191:CDS
TATTTCGAGACGACATGCAA+AGG 0.556622 8.4:+58408297 None:intergenic
AAATAATCATATGACAATTG+TGG 0.557071 8.4:+58407629 None:intergenic
AACCTTCTTTACAATGCAGT+CGG 0.562989 8.4:-58407169 MS.gene87191:CDS
TTAAAAGCCAATGTAGAGGC+CGG 0.565401 8.4:-58407779 MS.gene87191:CDS
GTTGGACGTGTTCACTACCG+TGG 0.566372 8.4:+58408372 None:intergenic
CTTTGTTAAGGCTGATAGTG+TGG 0.569170 8.4:-58407447 MS.gene87191:CDS
GTGATCCGTTCCAAACAACT+TGG 0.569439 8.4:-58407704 MS.gene87191:CDS
TAAAATTGAGTTAACATCGT+GGG 0.569675 8.4:-58408449 MS.gene87191:CDS
AAGGAGCTATTAGAAGAAGG+TGG 0.571952 8.4:-58407566 MS.gene87191:CDS
TCCATCGCATGCAACAATGC+CGG 0.578735 8.4:-58408244 MS.gene87191:CDS
ACCGGCATTGTTGCATGCGA+TGG 0.578867 8.4:+58408243 None:intergenic
AAGGAAGGTATGTGGATCCA+AGG 0.580681 8.4:+58408173 None:intergenic
ATTTGGTGCCTTAATAGTAG+TGG 0.582575 8.4:+58408492 None:intergenic
CTACCACTTCGAATTGCCAC+AGG 0.585255 8.4:+58407350 None:intergenic
CGGGGTTACCATGAAAGTCA+AGG 0.587965 8.4:-58407585 MS.gene87191:CDS
GAAAACAGAAGGAAGGTATG+TGG 0.590518 8.4:+58408165 None:intergenic
GATCACTGAGCGCCAAAGGT+GGG 0.592080 8.4:+58407721 None:intergenic
AGTAATTCCTTGGTCATCAG+TGG 0.592810 8.4:-58407419 MS.gene87191:CDS
ATATTGTAAATACTGAACAA+TGG 0.602893 8.4:+58407392 None:intergenic
TTTGGTTGGATCCAAACGGA+AGG 0.604535 8.4:+58408416 None:intergenic
GGATCACTGAGCGCCAAAGG+TGG 0.611585 8.4:+58407720 None:intergenic
GGTTAAGTGGAAAGAAGGAG+TGG 0.615609 8.4:+58408143 None:intergenic
AGGAGCTATTAGAAGAAGGT+GGG 0.616873 8.4:-58407565 MS.gene87191:CDS
AAAATTAAAAGCCAATGTAG+AGG 0.618767 8.4:-58407783 MS.gene87191:CDS
CATCGATCATGTTTAACTCA+GGG 0.619384 8.4:+58407219 None:intergenic
TTATGAAAGGAAATCGTATG+GGG 0.619650 8.4:+58407900 None:intergenic
TGAGTTGTTCTTCATCAGAA+CGG 0.620984 8.4:+58407855 None:intergenic
GTTACTTGGAATGCAAGCAA+TGG 0.622375 8.4:+58408091 None:intergenic
AATAAGCGTTCAAGTGTTGG+AGG 0.635161 8.4:+58407941 None:intergenic
GTATGTGGATCCAAGGATGT+CGG 0.635698 8.4:+58408180 None:intergenic
AAATGCTATGATAGTTTGCG+GGG 0.636971 8.4:-58407603 MS.gene87191:CDS
TGCATGAACAAAGAGTGCAC+CGG 0.640901 8.4:+58408225 None:intergenic
AAGAAGGTTGTGGAGGATCG+AGG 0.642827 8.4:+58407183 None:intergenic
CATTGTAAAGAAGGTTGTGG+AGG 0.644905 8.4:+58407176 None:intergenic
TGGGGAACCACTGATGACCA+AGG 0.650837 8.4:+58407412 None:intergenic
ACATTCCATCAATGACAACA+TGG 0.651969 8.4:+58408029 None:intergenic
TCGAGACGACATGCAAAGGG+TGG 0.652780 8.4:+58408301 None:intergenic
AACCGAATTATAAACTACGA+AGG 0.653559 8.4:-58408124 MS.gene87191:CDS
ACGTGTTCACTACCGTGGGA+TGG 0.666792 8.4:+58408377 None:intergenic
AATCCTGTGGCAATTCGAAG+TGG 0.668136 8.4:-58407353 MS.gene87191:CDS
GTTTCTTTATCACCATCCCA+CGG 0.670189 8.4:-58408389 MS.gene87191:CDS
TTGGACGTGTTCACTACCGT+GGG 0.674752 8.4:+58408373 None:intergenic
TCATTGGCTGAAATCTCACG+TGG 0.705667 8.4:-58407986 MS.gene87191:CDS
ATTGTAAATACTGAACAATG+GGG 0.706549 8.4:+58407394 None:intergenic
TATTGGTTGGGGAAATCCTG+TGG 0.714145 8.4:-58407366 MS.gene87191:CDS
GTAAAATTGAGTTAACATCG+TGG 0.722445 8.4:-58408450 MS.gene87191:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTATTTACAATATTGATAT+TGG - chr8.4:58408297-58408316 MS.gene87191:CDS 15.0%
!! AAATAATCATATGACAATTG+TGG + chr8.4:58408054-58408073 None:intergenic 20.0%
!! ATATTGTAAATACTGAACAA+TGG + chr8.4:58408291-58408310 None:intergenic 20.0%
!! CAATTGTCATATGATTATTT+AGG - chr8.4:58408054-58408073 MS.gene87191:CDS 20.0%
!! TATTGTAAATACTGAACAAT+GGG + chr8.4:58408290-58408309 None:intergenic 20.0%
!! TTTACAATATTGATATTGGT+TGG - chr8.4:58408301-58408320 MS.gene87191:CDS 20.0%
!!! TTACAATATTGATATTGGTT+GGG - chr8.4:58408302-58408321 MS.gene87191:CDS 20.0%
! AAAATTAAAAGCCAATGTAG+AGG - chr8.4:58407897-58407916 MS.gene87191:CDS 25.0%
! ATTATGAAAGGAAATCGTAT+GGG + chr8.4:58407784-58407803 None:intergenic 25.0%
! ATTGTAAATACTGAACAATG+GGG + chr8.4:58408289-58408308 None:intergenic 25.0%
! GGTAGTTCAAATAAAAGTAA+TGG - chr8.4:58408348-58408367 MS.gene87191:CDS 25.0%
! TAAAATTGAGTTAACATCGT+GGG - chr8.4:58407231-58407250 MS.gene87191:CDS 25.0%
! TAAACAGTCAATATGAATCT+TGG - chr8.4:58408196-58408215 MS.gene87191:CDS 25.0%
! TACCTTCGTAGTTTATAATT+CGG + chr8.4:58407561-58407580 None:intergenic 25.0%
! TAGTTTATAATTCGGTTAAG+TGG + chr8.4:58407553-58407572 None:intergenic 25.0%
! TATGTATAAGTGTTAGATCA+AGG - chr8.4:58408022-58408041 MS.gene87191:CDS 25.0%
! TATTATGAAAGGAAATCGTA+TGG + chr8.4:58407785-58407804 None:intergenic 25.0%
! TGATATTCTCCTTTGTAAAA+TGG + chr8.4:58407870-58407889 None:intergenic 25.0%
!! GAGAATATTCCATTTTACAA+AGG - chr8.4:58407858-58407877 MS.gene87191:CDS 25.0%
!! TACAATATTGATATTGGTTG+GGG - chr8.4:58408303-58408322 MS.gene87191:CDS 25.0%
!!! TGTGGTGTTTTTATTATGAA+AGG + chr8.4:58407796-58407815 None:intergenic 25.0%
!!! TTTAAAGTGTTGGATTTTGT+TGG + chr8.4:58407329-58407348 None:intergenic 25.0%
AAATGGAATATTCTCTCAGT+TGG + chr8.4:58407853-58407872 None:intergenic 30.0%
AACACAAAATTCACTTCTTG+TGG + chr8.4:58408003-58408022 None:intergenic 30.0%
AACCGAATTATAAACTACGA+AGG - chr8.4:58407556-58407575 MS.gene87191:CDS 30.0%
AAGAACACCAAACTTTGTTA+AGG - chr8.4:58408221-58408240 MS.gene87191:CDS 30.0%
AATGGAATATTCTCTCAGTT+GGG + chr8.4:58407852-58407871 None:intergenic 30.0%
ACACAAAATTCACTTCTTGT+GGG + chr8.4:58408002-58408021 None:intergenic 30.0%
GTAAAATTGAGTTAACATCG+TGG - chr8.4:58407230-58407249 MS.gene87191:CDS 30.0%
TATAAGTGTTAGATCAAGGT+TGG - chr8.4:58408026-58408045 MS.gene87191:CDS 30.0%
TGAATTGATGAAATGCGAAT+TGG + chr8.4:58407677-58407696 None:intergenic 30.0%
TGGAAAGAATGCAAAAACAT+GGG + chr8.4:58407362-58407381 None:intergenic 30.0%
TTATGAAAGGAAATCGTATG+GGG + chr8.4:58407783-58407802 None:intergenic 30.0%
TTCGCATTTCATCAATTCAT+TGG - chr8.4:58407678-58407697 MS.gene87191:CDS 30.0%
! AAAGAGACTGTTTAAAGTGT+TGG + chr8.4:58407339-58407358 None:intergenic 30.0%
! TTGCATGGAAGATATTTTGT+TGG + chr8.4:58407929-58407948 None:intergenic 30.0%
AACCTTCTTTACAATGCAGT+CGG - chr8.4:58408511-58408530 MS.gene87191:CDS 35.0%
AATTCGGTTAAGTGGAAAGA+AGG + chr8.4:58407545-58407564 None:intergenic 35.0%
ACATTCCATCAATGACAACA+TGG + chr8.4:58407654-58407673 None:intergenic 35.0%
ATGAACCATGTTGTCATTGA+TGG - chr8.4:58407646-58407665 MS.gene87191:CDS 35.0%
ATGACCTCCAAAATCTTGTA+TGG + chr8.4:58408432-58408451 None:intergenic 35.0%
CAAGTAACAGAGTTAGTTGA+CGG - chr8.4:58407604-58407623 MS.gene87191:CDS 35.0%
CATCGATCATGTTTAACTCA+GGG + chr8.4:58408464-58408483 None:intergenic 35.0%
CTATCAGCCTTAACAAAGTT+TGG + chr8.4:58408231-58408250 None:intergenic 35.0%
CTTGAACGCTTATTTCCAAA+CGG - chr8.4:58407748-58407767 MS.gene87191:CDS 35.0%
GATGACCAAGGAATTACTAT+TGG + chr8.4:58408259-58408278 None:intergenic 35.0%
GGAAATGCTATGATAGTTTG+CGG - chr8.4:58408075-58408094 MS.gene87191:CDS 35.0%
GTGGAAAGAATGCAAAAACA+TGG + chr8.4:58407363-58407382 None:intergenic 35.0%
TAATAGCTCCTTGACTTTCA+TGG + chr8.4:58408106-58408125 None:intergenic 35.0%
TCTCTCTACCACTACTATTA+AGG - chr8.4:58407180-58407199 MS.gene87191:CDS 35.0%
TGAGTTGTTCTTCATCAGAA+CGG + chr8.4:58407828-58407847 None:intergenic 35.0%
TGGTAAAGAAGAAGGAAGTA+TGG - chr8.4:58408389-58408408 MS.gene87191:CDS 35.0%
TTAGTTGAGTCACCTACATT+TGG + chr8.4:58407208-58407227 None:intergenic 35.0%
! AAATGCTATGATAGTTTGCG+GGG - chr8.4:58408077-58408096 MS.gene87191:CDS 35.0%
! ACTATTAAGGCACCAAATGT+AGG - chr8.4:58407193-58407212 MS.gene87191:CDS 35.0%
! ATAAAGAAACCCCTTTTGGT+TGG + chr8.4:58407281-58407300 None:intergenic 35.0%
! GAAATGCTATGATAGTTTGC+GGG - chr8.4:58408076-58408095 MS.gene87191:CDS 35.0%
! GGAAGATATTTTGTTGGTAC+CGG + chr8.4:58407923-58407942 None:intergenic 35.0%
! GTGTCTTCCATACAAGATTT+TGG - chr8.4:58408422-58408441 MS.gene87191:CDS 35.0%
! TCAGAACGGATCTTAATTTG+TGG + chr8.4:58407814-58407833 None:intergenic 35.0%
! TCTTCCATACAAGATTTTGG+AGG - chr8.4:58408425-58408444 MS.gene87191:CDS 35.0%
! TTGACGGTACTTTTGTTAGT+TGG - chr8.4:58407620-58407639 MS.gene87191:CDS 35.0%
! TTGGAGGTACAGATATTTGA+TGG + chr8.4:58407726-58407745 None:intergenic 35.0%
!! ATACAAGATTTTGGAGGTCA+TGG - chr8.4:58408431-58408450 MS.gene87191:CDS 35.0%
!! ATTTGGTGCCTTAATAGTAG+TGG + chr8.4:58407191-58407210 None:intergenic 35.0%
!! TACAAGATTTTGGAGGTCAT+GGG - chr8.4:58408432-58408451 MS.gene87191:CDS 35.0%
AACCGACTGCATTGTAAAGA+AGG + chr8.4:58408516-58408535 None:intergenic 40.0%
AAGAAGGAGTGGAAAACAGA+AGG + chr8.4:58407529-58407548 None:intergenic 40.0%
AAGGAGCTATTAGAAGAAGG+TGG - chr8.4:58408114-58408133 MS.gene87191:CDS 40.0%
ACATGATCGATGCATCTACT+TGG - chr8.4:58408472-58408491 MS.gene87191:CDS 40.0%
AGGAGCTATTAGAAGAAGGT+GGG - chr8.4:58408115-58408134 MS.gene87191:CDS 40.0%
AGTAATTCCTTGGTCATCAG+TGG - chr8.4:58408261-58408280 MS.gene87191:CDS 40.0%
ATTTCGAGACGACATGCAAA+GGG + chr8.4:58407385-58407404 None:intergenic 40.0%
CATTGTAAAGAAGGTTGTGG+AGG + chr8.4:58408507-58408526 None:intergenic 40.0%
CGTCAACTAACTCTGTTACT+TGG + chr8.4:58407606-58407625 None:intergenic 40.0%
CTGCATTGTAAAGAAGGTTG+TGG + chr8.4:58408510-58408529 None:intergenic 40.0%
GGAAAGATTAGTGTGTTTGC+TGG - chr8.4:58408369-58408388 MS.gene87191:CDS 40.0%
GGAAATAAGCGTTCAAGTGT+TGG + chr8.4:58407745-58407764 None:intergenic 40.0%
GTCAAGGAGCTATTAGAAGA+AGG - chr8.4:58408111-58408130 MS.gene87191:CDS 40.0%
GTTACTTGGAATGCAAGCAA+TGG + chr8.4:58407592-58407611 None:intergenic 40.0%
GTTTCTTTATCACCATCCCA+CGG - chr8.4:58407291-58407310 MS.gene87191:CDS 40.0%
TATTTCGAGACGACATGCAA+AGG + chr8.4:58407386-58407405 None:intergenic 40.0%
TGTGGCCAATAGTAATTCCT+TGG - chr8.4:58408251-58408270 MS.gene87191:CDS 40.0%
TTAAAAGCCAATGTAGAGGC+CGG - chr8.4:58407901-58407920 MS.gene87191:CDS 40.0%
! CTTTGTTAAGGCTGATAGTG+TGG - chr8.4:58408233-58408252 MS.gene87191:CDS 40.0%
! GAAAACAGAAGGAAGGTATG+TGG + chr8.4:58407518-58407537 None:intergenic 40.0%
! GCATCGATCATGTTTAACTC+AGG + chr8.4:58408465-58408484 None:intergenic 40.0%
! GGTGATAAAGAAACCCCTTT+TGG + chr8.4:58407285-58407304 None:intergenic 40.0%
! GTGTTTGCTGGTAAAGAAGA+AGG - chr8.4:58408381-58408400 MS.gene87191:CDS 40.0%
!! AATAAGCGTTCAAGTGTTGG+AGG + chr8.4:58407742-58407761 None:intergenic 40.0%
AATCCTGTGGCAATTCGAAG+TGG - chr8.4:58408327-58408346 MS.gene87191:CDS 45.0%
AGGAGTGGAAAACAGAAGGA+AGG + chr8.4:58407525-58407544 None:intergenic 45.0%
CCGTTTGGATCCAACCAAAA+GGG - chr8.4:58407268-58407287 MS.gene87191:CDS 45.0%
CGTTTGGATCCAACCAAAAG+GGG - chr8.4:58407269-58407288 MS.gene87191:CDS 45.0%
GATCTTCAGTTCCTTCCGTT+TGG - chr8.4:58407253-58407272 MS.gene87191:CDS 45.0%
GGTTAAGTGGAAAGAAGGAG+TGG + chr8.4:58407540-58407559 None:intergenic 45.0%
GTATGTGGATCCAAGGATGT+CGG + chr8.4:58407503-58407522 None:intergenic 45.0%
GTGATCCGTTCCAAACAACT+TGG - chr8.4:58407976-58407995 MS.gene87191:CDS 45.0%
TCCGTTTGGATCCAACCAAA+AGG - chr8.4:58407267-58407286 MS.gene87191:CDS 45.0%
TTCTTGTGGGCCAAGTTGTT+TGG + chr8.4:58407989-58408008 None:intergenic 45.0%
TTTGGTTGGATCCAAACGGA+AGG + chr8.4:58407267-58407286 None:intergenic 45.0%
! AAGGAAGGTATGTGGATCCA+AGG + chr8.4:58407510-58407529 None:intergenic 45.0%
! CTATTAGAAGAAGGTGGGCT+TGG - chr8.4:58408120-58408139 MS.gene87191:CDS 45.0%
! TATTGGTTGGGGAAATCCTG+TGG - chr8.4:58408314-58408333 MS.gene87191:CDS 45.0%
! TCATTGGCTGAAATCTCACG+TGG - chr8.4:58407694-58407713 MS.gene87191:CDS 45.0%
! TGCATGAACAAAGAGTGCAC+CGG + chr8.4:58407458-58407477 None:intergenic 45.0%
!! CCCTTTTGGTTGGATCCAAA+CGG + chr8.4:58407271-58407290 None:intergenic 45.0%
AAGAAGGTTGTGGAGGATCG+AGG + chr8.4:58408500-58408519 None:intergenic 50.0%
ACATGTGTTGCCGACATCCT+TGG - chr8.4:58407490-58407509 MS.gene87191:CDS 50.0%
CGGGGTTACCATGAAAGTCA+AGG - chr8.4:58408095-58408114 MS.gene87191:CDS 50.0%
CTACCACTTCGAATTGCCAC+AGG + chr8.4:58408333-58408352 None:intergenic 50.0%
GTGGGCCAAGTTGTTTGGAA+CGG + chr8.4:58407984-58408003 None:intergenic 50.0%
TCCATCGCATGCAACAATGC+CGG - chr8.4:58407436-58407455 MS.gene87191:CDS 50.0%
TTGGACGTGTTCACTACCGT+GGG + chr8.4:58407310-58407329 None:intergenic 50.0%
! GAAGAAGGTGGGCTTGGTAA+AGG - chr8.4:58408126-58408145 MS.gene87191:CDS 50.0%
AACGGATCACTGAGCGCCAA+AGG + chr8.4:58407966-58407985 None:intergenic 55.0%
AAGCGCTTTGCACCCACCTT+TGG - chr8.4:58407947-58407966 MS.gene87191:CDS 55.0%
ACCGGCATTGTTGCATGCGA+TGG + chr8.4:58407440-58407459 None:intergenic 55.0%
ACGTGTTCACTACCGTGGGA+TGG + chr8.4:58407306-58407325 None:intergenic 55.0%
GATCACTGAGCGCCAAAGGT+GGG + chr8.4:58407962-58407981 None:intergenic 55.0%
GTTGGACGTGTTCACTACCG+TGG + chr8.4:58407311-58407330 None:intergenic 55.0%
TCGAGACGACATGCAAAGGG+TGG + chr8.4:58407382-58407401 None:intergenic 55.0%
TGGGGAACCACTGATGACCA+AGG + chr8.4:58408271-58408290 None:intergenic 55.0%
!! ATGGGGCGCTGAATTCCGTT+TGG + chr8.4:58407766-58407785 None:intergenic 55.0%
!! GTTGGTACCGGCCTCTACAT+TGG + chr8.4:58407911-58407930 None:intergenic 55.0%
GGATCACTGAGCGCCAAAGG+TGG + chr8.4:58407963-58407982 None:intergenic 60.0%
! GTGGGTGCAAAGCGCTTGCA+TGG + chr8.4:58407944-58407963 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 58407163 58408539 58407163 ID=MS.gene87191
chr8.4 mRNA 58407163 58408539 58407163 ID=MS.gene87191.t1;Parent=MS.gene87191
chr8.4 exon 58407163 58408539 58407163 ID=MS.gene87191.t1.exon1;Parent=MS.gene87191.t1
chr8.4 CDS 58407163 58408539 58407163 ID=cds.MS.gene87191.t1;Parent=MS.gene87191.t1
Gene Sequence

>MS.gene87191

ATGAGTGCTTTTCGAGTTCTCTCTACCACTACTATTAAGGCACCAAATGTAGGTGACTCAACTAATCGTAAAATTGAGTTAACATCGTGGGATCTTCAGTTCCTTCCGTTTGGATCCAACCAAAAGGGGTTTCTTTATCACCATCCCACGGTAGTGAACACGTCCAACAAAATCCAACACTTTAAACAGTCTCTTTCCCATGTTTTTGCATTCTTTCCACCCTTTGCATGTCGTCTCGAAATAACTAAACATGAAGATAACACTATCTCGTGTTCCATCGCATGCAACAATGCCGGTGCACTCTTTGTTCATGCAAAAGCAGAGAATACATGTGTTGCCGACATCCTTGGATCCACATACCTTCCTTCTGTTTTCCACTCCTTCTTTCCACTTAACCGAATTATAAACTACGAAGGTACATCACATCCATTGCTTGCATTCCAAGTAACAGAGTTAGTTGACGGTACTTTTGTTAGTTGGACAATGAACCATGTTGTCATTGATGGAATGTCCAATTCGCATTTCATCAATTCATTGGCTGAAATCTCACGTGGTTCTTGCCATCAAATATCTGTACCTCCAACACTTGAACGCTTATTTCCAAACGGAATTCAGCGCCCCATACGATTTCCTTTCATAATAAAAACACCACAAATTAAGATCCGTTCTGATGAAGAACAACTCATCCCAACTGAGAGAATATTCCATTTTACAAAGGAGAATATCATGAGAATAAAATTAAAAGCCAATGTAGAGGCCGGTACCAACAAAATATCTTCCATGCAAGCGCTTTGCACCCACCTTTGGCGCTCAGTGATCCGTTCCAAACAACTTGGCCCACAAGAAGTGAATTTTGTGTTATGTATAAGTGTTAGATCAAGGTTGGTTCCACAATTGTCATATGATTATTTAGGAAATGCTATGATAGTTTGCGGGGTTACCATGAAAGTCAAGGAGCTATTAGAAGAAGGTGGGCTTGGTAAAGGTGCTTTAGAGATGTACAAGATCATTGCTTTGCACTCTGATGAGAAGTTAAACAGTCAATATGAATCTTGGTCAAGAACACCAAACTTTGTTAAGGCTGATAGTGTGGCCAATAGTAATTCCTTGGTCATCAGTGGTTCCCCATTGTTCAGTATTTACAATATTGATATTGGTTGGGGAAATCCTGTGGCAATTCGAAGTGGTAGTTCAAATAAAAGTAATGGAAAGATTAGTGTGTTTGCTGGTAAAGAAGAAGGAAGTATGGACTTTGAAGTGTGTCTTCCATACAAGATTTTGGAGGTCATGGGAAATGACCCTGAGTTAAACATGATCGATGCATCTACTTGGAACCTCGATCCTCCACAACCTTCTTTACAATGCAGTCGGTTGTAG
Protein sequence

>MS.gene87191.t1

MSAFRVLSTTTIKAPNVGDSTNRKIELTSWDLQFLPFGSNQKGFLYHHPTVVNTSNKIQHFKQSLSHVFAFFPPFACRLEITKHEDNTISCSIACNNAGALFVHAKAENTCVADILGSTYLPSVFHSFFPLNRIINYEGTSHPLLAFQVTELVDGTFVSWTMNHVVIDGMSNSHFINSLAEISRGSCHQISVPPTLERLFPNGIQRPIRFPFIIKTPQIKIRSDEEQLIPTERIFHFTKENIMRIKLKANVEAGTNKISSMQALCTHLWRSVIRSKQLGPQEVNFVLCISVRSRLVPQLSYDYLGNAMIVCGVTMKVKELLEEGGLGKGALEMYKIIALHSDEKLNSQYESWSRTPNFVKADSVANSNSLVISGSPLFSIYNIDIGWGNPVAIRSGSSNKSNGKISVFAGKEEGSMDFEVCLPYKILEVMGNDPELNMIDASTWNLDPPQPSLQCSRL