AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02573

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02573.t1 MTR_3g088420 94.457 433 24 0 1 433 1 433 0.0 859
MS.gene02573.t1 MTR_3g026310 72.098 448 110 3 1 433 1 448 0.0 662
MS.gene02573.t1 MTR_4g022800 68.778 442 129 3 1 433 1 442 0.0 617
MS.gene02573.t1 MTR_4g022670 67.347 441 136 2 1 433 1 441 0.0 614
MS.gene02573.t1 MTR_4g022825 67.500 440 136 1 1 433 1 440 0.0 609
MS.gene02573.t1 MTR_4g022720 68.046 435 137 2 1 433 1 435 0.0 602
MS.gene02573.t1 MTR_7g026860 66.440 441 137 3 1 433 1 438 0.0 588
MS.gene02573.t1 MTR_8g041770 61.261 444 158 6 1 433 1 441 0.0 536
MS.gene02573.t1 MTR_0190s0020 60.811 444 161 6 1 433 1 442 0.0 531
MS.gene02573.t1 MTR_4g022760 60.317 441 123 3 1 433 1 397 0.0 519
MS.gene02573.t1 MTR_4g094842 56.098 451 173 9 1 431 1 446 2.73e-168 480
MS.gene02573.t1 MTR_4g094835 54.018 448 181 10 1 429 1 442 1.28e-162 466
MS.gene02573.t1 MTR_6g016815 54.965 433 129 6 1 433 15 381 3.13e-152 437
MS.gene02573.t1 MTR_4g094828 51.209 455 197 11 1 434 1 451 3.46e-151 437
MS.gene02573.t1 MTR_8g079710 53.691 447 183 12 1 429 1 441 3.48e-149 431
MS.gene02573.t1 MTR_4g094838 51.448 449 174 9 4 429 1 428 2.44e-148 429
MS.gene02573.t1 MTR_4g022290 51.220 451 190 11 1 430 1 442 1.01e-146 425
MS.gene02573.t1 MTR_4g094832 53.867 375 154 6 69 429 57 426 1.67e-134 394
MS.gene02573.t1 MTR_3g101900 39.261 433 240 10 18 434 24 449 2.24e-93 289
MS.gene02573.t1 MTR_8g040780 63.229 223 78 2 210 428 5 227 2.83e-93 281
MS.gene02573.t1 MTR_8g040760 64.623 212 69 2 1 206 1 212 3.79e-90 272
MS.gene02573.t1 MTR_3g102020 38.073 436 245 10 18 434 23 452 1.34e-86 271
MS.gene02573.t1 MTR_3g101930 37.701 435 247 9 18 432 24 454 7.83e-86 270
MS.gene02573.t1 MTR_3g102010 37.931 435 246 10 18 434 24 452 1.04e-83 264
MS.gene02573.t1 MTR_3g101970 37.355 431 241 9 18 434 23 438 3.93e-83 262
MS.gene02573.t1 MTR_3g101960 36.512 430 247 10 18 428 23 445 1.57e-79 253
MS.gene02573.t1 MTR_7g026850 70.968 155 45 0 29 183 12 166 3.01e-77 238
MS.gene02573.t1 MTR_3g070170 30.962 478 267 13 2 433 10 470 5.28e-62 208
MS.gene02573.t1 MTR_6g034485 72.581 124 33 1 189 312 3 125 1.94e-58 187
MS.gene02573.t1 MTR_7g026770 49.362 235 77 4 109 322 80 293 3.75e-58 193
MS.gene02573.t1 MTR_5g081960 30.962 478 251 12 19 429 17 482 1.04e-57 197
MS.gene02573.t1 MTR_4g015180 33.610 482 241 16 17 433 20 487 2.87e-57 196
MS.gene02573.t1 MTR_4g094825 56.886 167 65 4 16 176 83 248 2.99e-53 183
MS.gene02573.t1 MTR_4g094825 51.656 151 60 2 285 433 252 391 1.01e-41 152
MS.gene02573.t1 MTR_6g045553 30.425 447 268 11 20 429 31 471 1.90e-49 175
MS.gene02573.t1 MTR_6g034495 61.314 137 43 3 49 180 33 164 5.23e-47 159
MS.gene02573.t1 MTR_3g102030 33.550 307 170 6 87 389 44 320 1.42e-45 160
MS.gene02573.t1 MTR_7g026830 71.429 84 24 0 82 165 14 97 7.80e-38 133
MS.gene02573.t1 MTR_8g096530 28.019 414 229 15 53 426 57 441 1.08e-31 125
MS.gene02573.t1 MTR_3g026950 27.322 366 222 12 52 385 51 404 1.32e-29 120
MS.gene02573.t1 MTR_7g026800 45.000 160 57 4 215 372 30 160 3.30e-29 113
MS.gene02573.t1 MTR_3g098980 27.066 351 209 11 55 385 66 389 7.21e-29 117
MS.gene02573.t1 MTR_8g075610 27.949 390 236 17 55 422 47 413 1.92e-28 116
MS.gene02573.t1 MTR_4g007540 26.110 383 246 14 55 417 56 421 2.31e-28 116
MS.gene02573.t1 MTR_4g022780 43.558 163 61 4 214 374 30 163 5.41e-28 109
MS.gene02573.t1 MTR_2g105330 25.916 382 243 15 55 415 56 418 1.03e-26 111
MS.gene02573.t1 MTR_7g026780 50.909 110 30 2 324 433 26 111 2.45e-26 102
MS.gene02573.t1 MTR_2g064495 25.654 382 244 15 55 415 56 418 2.75e-26 110
MS.gene02573.t1 MTR_3g049150 27.990 418 244 19 4 384 2 399 1.23e-25 108
MS.gene02573.t1 MTR_4g013050 25.419 358 205 9 49 385 47 363 4.14e-24 103
MS.gene02573.t1 MTR_4g097090 25.223 448 282 19 21 432 27 457 1.03e-22 100
MS.gene02573.t1 MTR_4g127650 25.362 414 264 15 49 432 59 457 1.10e-22 100
MS.gene02573.t1 MTR_7g014330 25.000 416 245 15 18 388 20 413 1.74e-20 94.0
MS.gene02573.t1 MTR_7g014250 25.416 421 250 16 14 388 11 413 4.18e-20 92.8
MS.gene02573.t1 MTR_7g085930 25.648 386 220 13 52 390 56 421 8.48e-19 89.0
MS.gene02573.t1 MTR_7g027175 24.365 394 267 12 50 431 63 437 8.63e-19 88.6
MS.gene02573.t1 MTR_2g089670 26.699 412 230 16 21 384 42 429 1.39e-18 88.2
MS.gene02573.t1 MTR_7g014230 26.379 417 238 15 18 388 22 415 2.40e-18 87.4
MS.gene02573.t1 MTR_7g026870 48.718 78 40 0 266 343 74 151 3.22e-18 81.6
MS.gene02573.t1 MTR_2g089755 25.000 436 259 19 24 409 37 454 5.13e-18 86.3
MS.gene02573.t1 MTR_6g015320 23.907 389 263 8 24 387 29 409 1.10e-17 85.5
MS.gene02573.t1 MTR_1g040195 26.366 421 261 18 25 427 23 412 2.27e-17 84.3
MS.gene02573.t1 MTR_5g076700 23.077 442 279 17 25 429 24 441 2.37e-17 84.3
MS.gene02573.t1 MTR_3g087640 25.734 443 276 16 22 420 29 462 5.68e-17 83.2
MS.gene02573.t1 MTR_7g026880 63.462 52 19 0 382 433 2 53 6.60e-17 74.7
MS.gene02573.t1 MTR_7g085990 25.817 306 201 8 52 337 52 351 1.06e-15 79.3
MS.gene02573.t1 MTR_2g089650 23.709 426 245 16 38 409 43 442 1.70e-15 78.6
MS.gene02573.t1 MTR_6g015315 24.759 311 204 8 138 423 140 445 2.00e-15 78.6
MS.gene02573.t1 MTR_7g015860 24.886 438 244 19 4 385 5 413 3.40e-15 77.8
MS.gene02573.t1 MTR_7g014200 24.762 420 240 16 22 388 28 424 4.24e-15 77.4
MS.gene02573.t1 MTR_3g086020 25.416 421 258 15 4 406 5 387 6.01e-15 76.6
MS.gene02573.t1 MTR_3g071320 25.397 315 186 12 54 343 59 349 9.74e-15 76.3
MS.gene02573.t1 MTR_7g114550 21.396 444 283 19 25 429 24 440 1.06e-14 76.3
MS.gene02573.t1 MTR_7g084910 28.881 277 168 11 49 308 62 326 1.26e-14 75.9
MS.gene02573.t1 MTR_1g053315 25.323 387 247 19 55 416 78 447 1.33e-14 75.9
MS.gene02573.t1 MTR_8g012750 24.818 411 249 15 22 384 24 422 1.36e-14 75.9
MS.gene02573.t1 MTR_7g114520 24.235 392 242 17 25 385 25 392 2.02e-14 75.5
MS.gene02573.t1 MTR_2g089765 23.934 422 254 14 15 384 28 434 3.27e-14 74.7
MS.gene02573.t1 MTR_1g040175 26.950 282 176 11 50 317 59 324 3.35e-14 74.7
MS.gene02573.t1 MTR_7g014620 24.476 429 257 17 24 414 15 414 1.23e-13 72.8
MS.gene02573.t1 MTR_4g055170 25.413 303 196 10 55 343 69 355 2.15e-13 72.0
MS.gene02573.t1 MTR_7g086000 30.000 260 160 12 58 302 63 315 2.22e-13 72.0
MS.gene02573.t1 MTR_3g465980 25.589 297 188 9 55 336 68 346 3.32e-13 71.6
MS.gene02573.t1 MTR_3g049140 27.039 233 142 10 204 414 6 232 1.63e-12 67.8
MS.gene02573.t1 MTR_0502s0010 28.571 217 141 8 110 317 14 225 2.19e-12 67.0
MS.gene02573.t1 MTR_5g090880 23.971 413 245 17 55 422 67 455 5.09e-12 67.8
MS.gene02573.t1 MTR_5g090810 24.372 398 236 17 32 385 36 412 5.49e-12 67.8
MS.gene02573.t1 MTR_3g465990 25.540 278 177 10 55 316 68 331 9.56e-12 67.0
MS.gene02573.t1 MTR_3g112180 23.898 431 275 18 10 420 29 426 1.42e-11 66.6
MS.gene02573.t1 MTR_5g090830 24.181 397 238 16 32 385 36 412 1.68e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02573.t1 AT5G67150 38.987 454 250 11 1 434 1 447 1.99e-97 299
MS.gene02573.t1 AT5G67160 40.183 438 237 11 6 434 12 433 2.30e-97 298
MS.gene02573.t1 AT3G50300 41.589 428 232 11 1 414 1 424 1.21e-95 295
MS.gene02573.t1 AT3G50270 39.775 445 250 11 6 434 7 449 2.63e-92 286
MS.gene02573.t1 AT3G50280 37.613 444 259 10 1 430 1 440 2.64e-91 283
MS.gene02573.t1 AT5G38130 39.627 429 237 11 1 414 21 442 1.78e-87 274
MS.gene02573.t1 AT5G42830 36.866 434 256 10 15 434 21 450 1.42e-85 269
MS.gene02573.t1 AT5G07860 38.118 425 240 12 24 432 31 448 1.50e-77 248
MS.gene02573.t1 AT5G07870 37.385 436 242 12 24 433 31 461 4.05e-76 245
MS.gene02573.t1 AT5G07850 35.828 441 252 13 15 433 22 453 2.74e-70 229
MS.gene02573.t1 AT3G50280 39.103 312 178 6 127 430 3 310 1.56e-65 213
MS.gene02573.t1 AT2G39980 32.495 477 245 14 19 433 19 480 6.76e-65 216
MS.gene02573.t1 AT3G50290 42.963 270 141 5 122 382 4 269 2.41e-61 200
MS.gene02573.t1 AT5G01210 32.683 410 221 11 66 433 73 469 1.50e-58 199
MS.gene02573.t1 AT5G23940 31.333 450 281 11 4 429 3 448 5.67e-57 195
MS.gene02573.t1 AT3G48720 27.616 344 217 10 55 385 61 385 1.84e-27 114
MS.gene02573.t1 AT5G48930 26.816 358 225 11 53 391 54 393 6.45e-27 112
MS.gene02573.t1 AT5G41040 25.937 347 227 10 52 385 66 395 4.12e-25 107
MS.gene02573.t1 AT5G41040 26.513 347 225 11 52 385 82 411 4.48e-25 107
MS.gene02573.t1 AT3G29590 25.223 448 299 14 2 424 6 442 2.67e-23 102
MS.gene02573.t1 AT5G57840 26.263 396 238 15 49 414 51 422 2.12e-21 96.3
MS.gene02573.t1 AT2G19070 25.120 418 258 19 50 431 53 451 2.58e-21 96.3
MS.gene02573.t1 AT3G29635 25.952 420 272 14 24 414 29 438 5.93e-21 95.1
MS.gene02573.t1 AT5G63560 24.857 350 215 11 55 385 61 381 1.36e-20 94.0
MS.gene02573.t1 AT3G26040 26.290 407 246 17 51 434 55 430 2.18e-20 93.6
MS.gene02573.t1 AT1G28680 24.503 453 282 19 1 427 1 419 1.03e-19 91.7
MS.gene02573.t1 AT3G29670 22.936 436 284 15 20 422 25 441 1.45e-18 88.2
MS.gene02573.t1 AT5G39050 27.273 352 188 15 122 420 132 468 4.83e-18 86.7
MS.gene02573.t1 AT1G24420 23.556 450 301 16 4 434 5 430 1.46e-17 84.7
MS.gene02573.t1 AT1G24420 23.556 450 301 16 4 434 5 430 1.46e-17 84.7
MS.gene02573.t1 AT1G28680 25.068 367 227 17 1 343 1 343 3.72e-17 83.2
MS.gene02573.t1 AT2G40230 27.887 355 216 15 54 389 58 391 4.01e-17 83.6
MS.gene02573.t1 AT5G39090 28.831 385 216 18 42 394 56 414 1.21e-15 79.0
MS.gene02573.t1 AT1G24430 24.603 378 219 16 54 406 61 397 2.00e-14 75.5
MS.gene02573.t1 AT1G03390 23.587 407 257 16 42 415 59 444 2.07e-14 75.5
MS.gene02573.t1 AT3G29680 24.619 394 247 13 24 385 25 400 6.38e-14 73.9
MS.gene02573.t1 AT5G61160 21.995 391 268 13 22 385 23 403 1.18e-13 73.2
MS.gene02573.t1 AT1G27620 23.097 381 263 13 55 422 58 421 3.36e-12 68.6
MS.gene02573.t1 AT1G78990 25.141 354 222 15 53 385 65 396 3.64e-12 68.6

Find 93 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCCGTGGTTTGATGTTTA+TGG 0.126200 3.2:+75327460 MS.gene02573:CDS
CCATTGCCTGAAGATTATTT+TGG 0.195668 3.2:+75327229 MS.gene02573:CDS
ATTGGCTAATGTATTATTAT+TGG 0.203749 3.2:-75327431 None:intergenic
GTGTCTTCCTCACAAGATTT+TGG 0.229479 3.2:+75327591 MS.gene02573:CDS
ACTAATTGCACCCACCAAAA+AGG 0.252147 3.2:+75326451 MS.gene02573:CDS
GATGTTTATGGTAATGATTT+TGG 0.308658 3.2:+75327472 MS.gene02573:CDS
AGTGTTTCAGATGTTGAATT+TGG 0.310563 3.2:-75326505 None:intergenic
ACGTTCTAGCGTTATGAGTT+TGG 0.324935 3.2:-75326909 None:intergenic
GCCTGCAAGTGGTGGGAAAA+AGG 0.338259 3.2:-75326546 None:intergenic
GTGGGCTTGGTAAAGGTGCT+TGG 0.384077 3.2:+75327308 MS.gene02573:CDS
ACACGAAGAAGTTTATTATA+TGG 0.390132 3.2:+75327174 MS.gene02573:CDS
TTCGTGTGGGTGAAATTGTT+TGG 0.393408 3.2:-75327158 None:intergenic
TTCAGATGTTGAATTTGGTT+TGG 0.398000 3.2:-75326500 None:intergenic
GCATTTCCAAAATAATCTTC+AGG 0.400923 3.2:-75327235 None:intergenic
AATTTGGTGACTTGATTGTA+TGG 0.401693 3.2:-75326382 None:intergenic
TTAGTTGACGGCGTCTTCAT+TGG 0.412859 3.2:+75326791 MS.gene02573:CDS
AATGAAAGCAGGACAGTTGT+TGG 0.413215 3.2:+75327279 MS.gene02573:CDS
GGAATGATTACTGTATTTGC+TGG 0.415451 3.2:+75327538 MS.gene02573:CDS
TTGTATGGGTAGAGAGGACT+TGG 0.418563 3.2:-75326367 None:intergenic
AAACCACGGGGAATTGCTAT+TGG 0.430657 3.2:-75327449 None:intergenic
GGGAAATGACCATGACTTCA+TGG 0.444511 3.2:+75327621 MS.gene02573:CDS
TGGAAAAGAAGAAGGTAGTA+TGG 0.447023 3.2:+75327558 MS.gene02573:CDS
GAAATGCTGTTGAAGTTTGT+AGG 0.451612 3.2:+75327251 MS.gene02573:CDS
TTTAACCACGCGGTTGTTGA+CGG 0.460621 3.2:+75326821 MS.gene02573:CDS
TTGAAAGCCAACATGGAGAT+TGG 0.465372 3.2:+75327073 MS.gene02573:CDS
CGCAGTGGAAATAAAATAAG+TGG 0.469980 3.2:+75327517 MS.gene02573:CDS
TGAAGAATCACTACGAGTCT+TGG 0.474671 3.2:+75327371 MS.gene02573:CDS
CCAAAATAATCTTCAGGCAA+TGG 0.482695 3.2:-75327229 None:intergenic
TGGGCTTGGTAAAGGTGCTT+GGG 0.485107 3.2:+75327309 MS.gene02573:CDS
ATTTAACTCCATGGGATCTC+CGG 0.488953 3.2:+75326423 MS.gene02573:CDS
GAAGATGGTGGGCTTGGTAA+AGG 0.490096 3.2:+75327301 MS.gene02573:CDS
ACCTTGATCTAGCTCCTATC+GGG 0.496130 3.2:-75327198 None:intergenic
AACCTTGATCTAGCTCCTAT+CGG 0.500296 3.2:-75327199 None:intergenic
CATGGCTGTTCATTAACTCA+TGG 0.501160 3.2:+75326852 MS.gene02573:CDS
GCATGTGACGGAACATGAAA+TGG 0.505364 3.2:-75326600 None:intergenic
AATCACTACGAGTCTTGGTT+GGG 0.505413 3.2:+75327376 MS.gene02573:CDS
GTTGGTACCAATCTCCATGT+TGG 0.507485 3.2:-75327080 None:intergenic
GAATCACTACGAGTCTTGGT+TGG 0.508104 3.2:+75327375 MS.gene02573:CDS
TGTGATCTGTTATTTCTAGA+CGG 0.512647 3.2:-75326568 None:intergenic
GCAGGACAGTTGTTGGAAGA+TGG 0.523872 3.2:+75327286 MS.gene02573:CDS
TATAGTGAAAGGAAATCGTA+TGG 0.523888 3.2:-75326957 None:intergenic
TACCATAAACATCAAACCAC+GGG 0.529973 3.2:-75327462 None:intergenic
TTTCCACTGCGAATTGCCAC+AGG 0.532396 3.2:-75327505 None:intergenic
TCTAGACGGCCTGCAAGTGG+TGG 0.533401 3.2:-75326554 None:intergenic
GACAGTTGTTGGAAGATGGT+GGG 0.539492 3.2:+75327290 MS.gene02573:CDS
AACTCATAACGCTAGAACGT+TGG 0.541457 3.2:+75326912 MS.gene02573:CDS
AAATGGAATAACCTCTCTGA+TGG 0.546561 3.2:-75327022 None:intergenic
TGTAGGGTTACAATGAAAGC+AGG 0.550470 3.2:+75327268 MS.gene02573:CDS
TTGTTGGAAGATGGTGGGCT+TGG 0.553654 3.2:+75327295 MS.gene02573:CDS
ACCAGCATTGTTGCATGTGA+CGG 0.553807 3.2:-75326612 None:intergenic
AAATGCTGTTGAAGTTTGTA+GGG 0.553867 3.2:+75327252 MS.gene02573:CDS
TCCCGATAGGAGCTAGATCA+AGG 0.559796 3.2:+75327197 MS.gene02573:CDS
GTATTTGCTGGAAAAGAAGA+AGG 0.567059 3.2:+75327550 MS.gene02573:CDS
AAATAATCTTCAGGCAATGG+TGG 0.571644 3.2:-75327226 None:intergenic
GGACAGTTGTTGGAAGATGG+TGG 0.574775 3.2:+75327289 MS.gene02573:CDS
AACGGATCACAGAGCGCCAT+AGG 0.575889 3.2:-75327135 None:intergenic
ACTGAAATTGAAAGCCAACA+TGG 0.579416 3.2:+75327066 MS.gene02573:CDS
ATGGCTGTTCATTAACTCAT+GGG 0.585409 3.2:+75326853 MS.gene02573:CDS
AAGTGTTCTTAGCCGCGACA+TGG 0.586192 3.2:-75326646 None:intergenic
ATTTCTAGACGGCCTGCAAG+TGG 0.587340 3.2:-75326557 None:intergenic
TGGGTGCAATTAGTAGGTAC+CGG 0.591093 3.2:-75326442 None:intergenic
GTTTATTATATGGTCCCGAT+AGG 0.594388 3.2:+75327184 MS.gene02573:CDS
ATATAATAAACTTCTTCGTG+TGG 0.595960 3.2:-75327172 None:intergenic
TATAATAAACTTCTTCGTGT+GGG 0.598129 3.2:-75327171 None:intergenic
TCCGTCACATGCAACAATGC+TGG 0.599979 3.2:+75326611 MS.gene02573:CDS
ACACAACATCCATGAAGTCA+TGG 0.605152 3.2:-75327630 None:intergenic
ATCGACGATGGGAGGAACGT+AGG 0.605346 3.2:-75326693 None:intergenic
CAAGTGACTGAGTTAGTTGA+CGG 0.606507 3.2:+75326779 MS.gene02573:CDS
AGTAGGTACCGGAGATCCCA+TGG 0.606618 3.2:-75326431 None:intergenic
ATTTGGTGACTTGATTGTAT+GGG 0.612690 3.2:-75326381 None:intergenic
ATGGGATGTTGAATACCACT+TGG 0.613611 3.2:-75326938 None:intergenic
ACTTGTGTAGGTGACATACT+TGG 0.616854 3.2:+75326665 MS.gene02573:CDS
GTGGGTGAAATTGTTTGGAA+CGG 0.621788 3.2:-75327153 None:intergenic
GCGGCTAAGAACACTTGTGT+AGG 0.621937 3.2:+75326653 MS.gene02573:CDS
TGAATTTGGTTTGGCACTAG+AGG 0.623195 3.2:-75326491 None:intergenic
CTAGACGGCCTGCAAGTGGT+GGG 0.623531 3.2:-75326553 None:intergenic
ATAGTGAAAGGAAATCGTAT+GGG 0.628856 3.2:-75326956 None:intergenic
ACTTGATTGTATGGGTAGAG+AGG 0.629120 3.2:-75326373 None:intergenic
GTCACTTGCACAGCTAACAA+TGG 0.634835 3.2:-75326764 None:intergenic
GAGGTTATTCCATTTCACAA+AGG 0.635344 3.2:+75327030 MS.gene02573:CDS
TTGTTGACGGAGAGTCCACA+TGG 0.635951 3.2:+75326834 MS.gene02573:CDS
TTTGGTTTGGCACTAGAGGA+TGG 0.637344 3.2:-75326487 None:intergenic
TCATGGGCTAAAATCTCGCG+TGG 0.637561 3.2:+75326869 MS.gene02573:CDS
AAACCTGTGGCAATTCGCAG+TGG 0.640198 3.2:+75327502 MS.gene02573:CDS
TTACCATAAACATCAAACCA+CGG 0.662908 3.2:-75327463 None:intergenic
ATTGCCTCCAAAATCTTGTG+AGG 0.672285 3.2:-75327598 None:intergenic
ATCGGAGTTCAAAACTACGA+AGG 0.674883 3.2:+75326731 MS.gene02573:CDS
TGCGCTCTTTGTCCATGTCG+CGG 0.682848 3.2:+75326634 MS.gene02573:CDS
CTAGAACGTTGGTTTCCAAG+TGG 0.685713 3.2:+75326923 MS.gene02573:CDS
TGAGTTAATGAACAGCCATG+TGG 0.699666 3.2:-75326849 None:intergenic
ACCATAAACATCAAACCACG+GGG 0.743607 3.2:-75327461 None:intergenic
TAGCCAATAGCAATTCCCCG+TGG 0.764549 3.2:+75327446 MS.gene02573:CDS
ACTCTCCGTCAACAACCGCG+TGG 0.786940 3.2:-75326826 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGGCTAATGTATTATTAT+TGG - chr3.2:75327434-75327453 None:intergenic 20.0%
!! CTAAAAAATCTAACAATGTT+TGG - chr3.2:75327406-75327425 None:intergenic 20.0%
!!! AAAAAATTGATTTAACTCCA+TGG + chr3.2:75326414-75326433 MS.gene02573:CDS 20.0%
!!! AAAAATTGATTTAACTCCAT+GGG + chr3.2:75326415-75326434 MS.gene02573:CDS 20.0%
!!! ATTTTTTGATCACTTGAATT+TGG - chr3.2:75326401-75326420 None:intergenic 20.0%
!!! GATTCATTTTTTCCATTAAT+CGG + chr3.2:75326713-75326732 MS.gene02573:CDS 20.0%
! ACACGAAGAAGTTTATTATA+TGG + chr3.2:75327174-75327193 MS.gene02573:CDS 25.0%
! ATATAATAAACTTCTTCGTG+TGG - chr3.2:75327175-75327194 None:intergenic 25.0%
! TATAATAAACTTCTTCGTGT+GGG - chr3.2:75327174-75327193 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr3.2:75327472-75327491 MS.gene02573:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG + chr3.2:75327477-75327496 MS.gene02573:CDS 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG + chr3.2:75327476-75327495 MS.gene02573:CDS 25.0%
AAAAATTCAATCCATCAGAG+AGG + chr3.2:75327011-75327030 MS.gene02573:CDS 30.0%
AAATGCTGTTGAAGTTTGTA+GGG + chr3.2:75327252-75327271 MS.gene02573:CDS 30.0%
AGTGTTTCAGATGTTGAATT+TGG - chr3.2:75326508-75326527 None:intergenic 30.0%
ATAGTGAAAGGAAATCGTAT+GGG - chr3.2:75326959-75326978 None:intergenic 30.0%
GCATTTCCAAAATAATCTTC+AGG - chr3.2:75327238-75327257 None:intergenic 30.0%
TATAGTGAAAGGAAATCGTA+TGG - chr3.2:75326960-75326979 None:intergenic 30.0%
TTACCATAAACATCAAACCA+CGG - chr3.2:75327466-75327485 None:intergenic 30.0%
TTCAGATGTTGAATTTGGTT+TGG - chr3.2:75326503-75326522 None:intergenic 30.0%
! AATTTGGTGACTTGATTGTA+TGG - chr3.2:75326385-75326404 None:intergenic 30.0%
! ATTTGGTGACTTGATTGTAT+GGG - chr3.2:75326384-75326403 None:intergenic 30.0%
!! TATGGTAATGATTTTGGATG+GGG + chr3.2:75327478-75327497 MS.gene02573:CDS 30.0%
!! TGTGATCTGTTATTTCTAGA+CGG - chr3.2:75326571-75326590 None:intergenic 30.0%
!!! TAGTTTTGAACTCCGATTAA+TGG - chr3.2:75326728-75326747 None:intergenic 30.0%
AAATAATCTTCAGGCAATGG+TGG - chr3.2:75327229-75327248 None:intergenic 35.0%
AAATGGAATAACCTCTCTGA+TGG - chr3.2:75327025-75327044 None:intergenic 35.0%
ACTGAAATTGAAAGCCAACA+TGG + chr3.2:75327066-75327085 MS.gene02573:CDS 35.0%
ATGGCTGTTCATTAACTCAT+GGG + chr3.2:75326853-75326872 MS.gene02573:CDS 35.0%
CCAAAATAATCTTCAGGCAA+TGG - chr3.2:75327232-75327251 None:intergenic 35.0%
CGCAGTGGAAATAAAATAAG+TGG + chr3.2:75327517-75327536 MS.gene02573:CDS 35.0%
GAAATGCTGTTGAAGTTTGT+AGG + chr3.2:75327251-75327270 MS.gene02573:CDS 35.0%
GAGGTTATTCCATTTCACAA+AGG + chr3.2:75327030-75327049 MS.gene02573:CDS 35.0%
GGAAAAAATGAATCGACGAT+GGG - chr3.2:75326707-75326726 None:intergenic 35.0%
GGAATGATTACTGTATTTGC+TGG + chr3.2:75327538-75327557 MS.gene02573:CDS 35.0%
GTTTATTATATGGTCCCGAT+AGG + chr3.2:75327184-75327203 MS.gene02573:CDS 35.0%
TACCATAAACATCAAACCAC+GGG - chr3.2:75327465-75327484 None:intergenic 35.0%
TGGAAAAAATGAATCGACGA+TGG - chr3.2:75326708-75326727 None:intergenic 35.0%
TGGAAAAGAAGAAGGTAGTA+TGG + chr3.2:75327558-75327577 MS.gene02573:CDS 35.0%
! ATAAAGAAGACCCTTTTTGG+TGG - chr3.2:75326465-75326484 None:intergenic 35.0%
! CCATTGCCTGAAGATTATTT+TGG + chr3.2:75327229-75327248 MS.gene02573:CDS 35.0%
! CGATTTTCTCCTTTGTGAAA+TGG - chr3.2:75327042-75327061 None:intergenic 35.0%
! GTATTTGCTGGAAAAGAAGA+AGG + chr3.2:75327550-75327569 MS.gene02573:CDS 35.0%
! GTGATAAAGAAGACCCTTTT+TGG - chr3.2:75326468-75326487 None:intergenic 35.0%
! TTGGAGGGATGATATTTTGT+TGG - chr3.2:75327101-75327120 None:intergenic 35.0%
!! AAGCACTTTTTACACACCTA+TGG + chr3.2:75327119-75327138 MS.gene02573:CDS 35.0%
!! ATAGGTGTGTAAAAAGTGCT+TGG - chr3.2:75327120-75327139 None:intergenic 35.0%
!! TAAAGAAGACCCTTTTTGGT+GGG - chr3.2:75326464-75326483 None:intergenic 35.0%
!! TCATCAGAGTCGTTATTTTG+TGG - chr3.2:75326986-75327005 None:intergenic 35.0%
!! TGGTTTTACATTTAACCACG+CGG + chr3.2:75326811-75326830 MS.gene02573:CDS 35.0%
!! TTTTGTGGCTCTATAGTGAA+AGG - chr3.2:75326971-75326990 None:intergenic 35.0%
AAAAATGAATCGACGATGGG+AGG - chr3.2:75326704-75326723 None:intergenic 40.0%
AACTCATAACGCTAGAACGT+TGG + chr3.2:75326912-75326931 MS.gene02573:CDS 40.0%
AATCACTACGAGTCTTGGTT+GGG + chr3.2:75327376-75327395 MS.gene02573:CDS 40.0%
AATGAAAGCAGGACAGTTGT+TGG + chr3.2:75327279-75327298 MS.gene02573:CDS 40.0%
ACACAACATCCATGAAGTCA+TGG - chr3.2:75327633-75327652 None:intergenic 40.0%
ACCATAAACATCAAACCACG+GGG - chr3.2:75327464-75327483 None:intergenic 40.0%
ACGTTCTAGCGTTATGAGTT+TGG - chr3.2:75326912-75326931 None:intergenic 40.0%
ACTAATTGCACCCACCAAAA+AGG + chr3.2:75326451-75326470 MS.gene02573:CDS 40.0%
ACTTGATTGTATGGGTAGAG+AGG - chr3.2:75326376-75326395 None:intergenic 40.0%
ACTTGTGTAGGTGACATACT+TGG + chr3.2:75326665-75326684 MS.gene02573:CDS 40.0%
ATCGGAGTTCAAAACTACGA+AGG + chr3.2:75326731-75326750 MS.gene02573:CDS 40.0%
ATGGGATGTTGAATACCACT+TGG - chr3.2:75326941-75326960 None:intergenic 40.0%
ATTGCCTCCAAAATCTTGTG+AGG - chr3.2:75327601-75327620 None:intergenic 40.0%
ATTTAACTCCATGGGATCTC+CGG + chr3.2:75326423-75326442 MS.gene02573:CDS 40.0%
CAAGTGACTGAGTTAGTTGA+CGG + chr3.2:75326779-75326798 MS.gene02573:CDS 40.0%
CATGGCTGTTCATTAACTCA+TGG + chr3.2:75326852-75326871 MS.gene02573:CDS 40.0%
CTAATTGCACCCACCAAAAA+GGG + chr3.2:75326452-75326471 MS.gene02573:CDS 40.0%
GTGGGTGAAATTGTTTGGAA+CGG - chr3.2:75327156-75327175 None:intergenic 40.0%
TGAAGAATCACTACGAGTCT+TGG + chr3.2:75327371-75327390 MS.gene02573:CDS 40.0%
TGAGTTAATGAACAGCCATG+TGG - chr3.2:75326852-75326871 None:intergenic 40.0%
TGTAGGGTTACAATGAAAGC+AGG + chr3.2:75327268-75327287 MS.gene02573:CDS 40.0%
TTCGTGTGGGTGAAATTGTT+TGG - chr3.2:75327161-75327180 None:intergenic 40.0%
TTGAAAGCCAACATGGAGAT+TGG + chr3.2:75327073-75327092 MS.gene02573:CDS 40.0%
! AACCTTGATCTAGCTCCTAT+CGG - chr3.2:75327202-75327221 None:intergenic 40.0%
! GTGTCTTCCTCACAAGATTT+TGG + chr3.2:75327591-75327610 MS.gene02573:CDS 40.0%
! TCTTCCTCACAAGATTTTGG+AGG + chr3.2:75327594-75327613 MS.gene02573:CDS 40.0%
!! CACAAGATTTTGGAGGCAAT+GGG + chr3.2:75327601-75327620 MS.gene02573:CDS 40.0%
!! GTGTGTAAAAAGTGCTTGGA+GGG - chr3.2:75327116-75327135 None:intergenic 40.0%
!! TCACAAGATTTTGGAGGCAA+TGG + chr3.2:75327600-75327619 MS.gene02573:CDS 40.0%
!! TGAATTTGGTTTGGCACTAG+AGG - chr3.2:75326494-75326513 None:intergenic 40.0%
!! TTTTGGTGGGTGCAATTAGT+AGG - chr3.2:75326451-75326470 None:intergenic 40.0%
AAACCACGGGGAATTGCTAT+TGG - chr3.2:75327452-75327471 None:intergenic 45.0%
ACCAGCATTGTTGCATGTGA+CGG - chr3.2:75326615-75326634 None:intergenic 45.0%
GAATCACTACGAGTCTTGGT+TGG + chr3.2:75327375-75327394 MS.gene02573:CDS 45.0%
GACAGTTGTTGGAAGATGGT+GGG + chr3.2:75327290-75327309 MS.gene02573:CDS 45.0%
GGGAAATGACCATGACTTCA+TGG + chr3.2:75327621-75327640 MS.gene02573:CDS 45.0%
GTCACTTGCACAGCTAACAA+TGG - chr3.2:75326767-75326786 None:intergenic 45.0%
GTTGGTACCAATCTCCATGT+TGG - chr3.2:75327083-75327102 None:intergenic 45.0%
TCCCCGTGGTTTGATGTTTA+TGG + chr3.2:75327460-75327479 MS.gene02573:CDS 45.0%
TGGGTGCAATTAGTAGGTAC+CGG - chr3.2:75326445-75326464 None:intergenic 45.0%
TTAGTTGACGGCGTCTTCAT+TGG + chr3.2:75326791-75326810 MS.gene02573:CDS 45.0%
TTGTATGGGTAGAGAGGACT+TGG - chr3.2:75326370-75326389 None:intergenic 45.0%
TTTAACCACGCGGTTGTTGA+CGG + chr3.2:75326821-75326840 MS.gene02573:CDS 45.0%
! AAGTGGTGGGAAAAAGGCAA+GGG - chr3.2:75326543-75326562 None:intergenic 45.0%
! ACCTTGATCTAGCTCCTATC+GGG - chr3.2:75327201-75327220 None:intergenic 45.0%
! GCATGTGACGGAACATGAAA+TGG - chr3.2:75326603-75326622 None:intergenic 45.0%
! TTTTGGATGGGGAAAACCTG+TGG + chr3.2:75327489-75327508 MS.gene02573:CDS 45.0%
!! CTAGAACGTTGGTTTCCAAG+TGG + chr3.2:75326923-75326942 MS.gene02573:CDS 45.0%
!! GGTGTGTAAAAAGTGCTTGG+AGG - chr3.2:75327117-75327136 None:intergenic 45.0%
!! TTTGGTTTGGCACTAGAGGA+TGG - chr3.2:75326490-75326509 None:intergenic 45.0%
AAACCTGTGGCAATTCGCAG+TGG + chr3.2:75327502-75327521 MS.gene02573:CDS 50.0%
AAGTGTTCTTAGCCGCGACA+TGG - chr3.2:75326649-75326668 None:intergenic 50.0%
GCAGGACAGTTGTTGGAAGA+TGG + chr3.2:75327286-75327305 MS.gene02573:CDS 50.0%
GCGGCTAAGAACACTTGTGT+AGG + chr3.2:75326653-75326672 MS.gene02573:CDS 50.0%
GGACAGTTGTTGGAAGATGG+TGG + chr3.2:75327289-75327308 MS.gene02573:CDS 50.0%
TAGCCAATAGCAATTCCCCG+TGG + chr3.2:75327446-75327465 MS.gene02573:CDS 50.0%
TCCCGATAGGAGCTAGATCA+AGG + chr3.2:75327197-75327216 MS.gene02573:CDS 50.0%
TCCGTCACATGCAACAATGC+TGG + chr3.2:75326611-75326630 MS.gene02573:CDS 50.0%
TTGTTGACGGAGAGTCCACA+TGG + chr3.2:75326834-75326853 MS.gene02573:CDS 50.0%
TTTCCACTGCGAATTGCCAC+AGG - chr3.2:75327508-75327527 None:intergenic 50.0%
! ATTTCTAGACGGCCTGCAAG+TGG - chr3.2:75326560-75326579 None:intergenic 50.0%
! CAAGTGGTGGGAAAAAGGCA+AGG - chr3.2:75326544-75326563 None:intergenic 50.0%
! TCATGGGCTAAAATCTCGCG+TGG + chr3.2:75326869-75326888 MS.gene02573:CDS 50.0%
!! GAAGATGGTGGGCTTGGTAA+AGG + chr3.2:75327301-75327320 MS.gene02573:CDS 50.0%
!! TGGGCTTGGTAAAGGTGCTT+GGG + chr3.2:75327309-75327328 MS.gene02573:CDS 50.0%
!! TTGTTGGAAGATGGTGGGCT+TGG + chr3.2:75327295-75327314 MS.gene02573:CDS 50.0%
ATCGACGATGGGAGGAACGT+AGG - chr3.2:75326696-75326715 None:intergenic 55.0%
TGCGCTCTTTGTCCATGTCG+CGG + chr3.2:75326634-75326653 MS.gene02573:CDS 55.0%
! AACGGATCACAGAGCGCCAT+AGG - chr3.2:75327138-75327157 None:intergenic 55.0%
! GCCTGCAAGTGGTGGGAAAA+AGG - chr3.2:75326549-75326568 None:intergenic 55.0%
! GCCTTTTTCCCACCACTTGC+AGG + chr3.2:75326545-75326564 MS.gene02573:CDS 55.0%
!! AGTAGGTACCGGAGATCCCA+TGG - chr3.2:75326434-75326453 None:intergenic 55.0%
!! GTGGGCTTGGTAAAGGTGCT+TGG + chr3.2:75327308-75327327 MS.gene02573:CDS 55.0%
ACTCTCCGTCAACAACCGCG+TGG - chr3.2:75326829-75326848 None:intergenic 60.0%
CTAGACGGCCTGCAAGTGGT+GGG - chr3.2:75326556-75326575 None:intergenic 60.0%
TCTAGACGGCCTGCAAGTGG+TGG - chr3.2:75326557-75326576 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 75326356 75327660 75326356 ID=MS.gene02573
chr3.2 mRNA 75326356 75327660 75326356 ID=MS.gene02573.t1;Parent=MS.gene02573
chr3.2 exon 75326356 75327660 75326356 ID=MS.gene02573.t1.exon1;Parent=MS.gene02573.t1
chr3.2 CDS 75326356 75327660 75326356 ID=cds.MS.gene02573.t1;Parent=MS.gene02573.t1
Gene Sequence

>MS.gene02573

ATGGCTGCAATCCAAGTCCTCTCTACCCATACAATCAAGTCACCAAATTCAAGTGATCAAAAAATTGATTTAACTCCATGGGATCTCCGGTACCTACTAATTGCACCCACCAAAAAGGGTCTTCTTTATCACCATCCTCTAGTGCCAAACCAAATTCAACATCTGAAACACTCTCTATCTTCTGCCCTTGCCTTTTTCCCACCACTTGCAGGCCGTCTAGAAATAACAGATCACAAAGACAACACCATTTCATGTTCCGTCACATGCAACAATGCTGGTGCGCTCTTTGTCCATGTCGCGGCTAAGAACACTTGTGTAGGTGACATACTTGGATGCACCTACGTTCCTCCCATCGTCGATTCATTTTTTCCATTAATCGGAGTTCAAAACTACGAAGGCACGTCACAACCATTGTTAGCTGTGCAAGTGACTGAGTTAGTTGACGGCGTCTTCATTGGTTTTACATTTAACCACGCGGTTGTTGACGGAGAGTCCACATGGCTGTTCATTAACTCATGGGCTAAAATCTCGCGTGGTTGTTGCAATCAAGTATCCAAACTCATAACGCTAGAACGTTGGTTTCCAAGTGGTATTCAACATCCCATACGATTTCCTTTCACTATAGAGCCACAAAATAACGACTCTGATGATAACGAAAAATTCAATCCATCAGAGAGGTTATTCCATTTCACAAAGGAGAAAATCGCTCAACTGAAATTGAAAGCCAACATGGAGATTGGTACCAACAAAATATCATCCCTCCAAGCACTTTTTACACACCTATGGCGCTCTGTGATCCGTTCCAAACAATTTCACCCACACGAAGAAGTTTATTATATGGTCCCGATAGGAGCTAGATCAAGGTTTGTTCCACCATTGCCTGAAGATTATTTTGGAAATGCTGTTGAAGTTTGTAGGGTTACAATGAAAGCAGGACAGTTGTTGGAAGATGGTGGGCTTGGTAAAGGTGCTTGGGCGATACACAAAATGATTTCTTTGCAGTCAAATGAGAAGTTGAAGAATCACTACGAGTCTTGGTTGGGAAATCCAAACATTGTTAGATTTTTTAGTGCGACCAATAATAATACATTAGCCAATAGCAATTCCCCGTGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAACCTGTGGCAATTCGCAGTGGAAATAAAATAAGTGGAATGATTACTGTATTTGCTGGAAAAGAAGAAGGTAGTATGGACTTTCAAGTGTGTCTTCCTCACAAGATTTTGGAGGCAATGGGAAATGACCATGACTTCATGGATGTTGTGTCTATTTAA
Protein sequence

>MS.gene02573.t1

MAAIQVLSTHTIKSPNSSDQKIDLTPWDLRYLLIAPTKKGLLYHHPLVPNQIQHLKHSLSSALAFFPPLAGRLEITDHKDNTISCSVTCNNAGALFVHVAAKNTCVGDILGCTYVPPIVDSFFPLIGVQNYEGTSQPLLAVQVTELVDGVFIGFTFNHAVVDGESTWLFINSWAKISRGCCNQVSKLITLERWFPSGIQHPIRFPFTIEPQNNDSDDNEKFNPSERLFHFTKEKIAQLKLKANMEIGTNKISSLQALFTHLWRSVIRSKQFHPHEEVYYMVPIGARSRFVPPLPEDYFGNAVEVCRVTMKAGQLLEDGGLGKGAWAIHKMISLQSNEKLKNHYESWLGNPNIVRFFSATNNNTLANSNSPWFDVYGNDFGWGKPVAIRSGNKISGMITVFAGKEEGSMDFQVCLPHKILEAMGNDHDFMDVVSI