AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066656

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066656.t1 MTR_4g022825 93.864 440 27 0 1 440 1 440 0.0 860
MS.gene066656.t1 MTR_4g022800 73.303 442 116 2 1 440 1 442 0.0 683
MS.gene066656.t1 MTR_4g022720 73.923 441 108 2 1 440 1 435 0.0 682
MS.gene066656.t1 MTR_4g022670 73.636 440 115 1 1 439 1 440 0.0 679
MS.gene066656.t1 MTR_7g026860 72.336 441 118 2 1 440 1 438 0.0 667
MS.gene066656.t1 MTR_3g088420 67.500 440 136 1 1 440 1 433 0.0 617
MS.gene066656.t1 MTR_3g026310 65.101 447 148 3 1 439 1 447 0.0 585
MS.gene066656.t1 MTR_4g022760 64.853 441 110 2 1 440 1 397 0.0 579
MS.gene066656.t1 MTR_8g041770 58.889 450 164 6 1 439 1 440 0.0 535
MS.gene066656.t1 MTR_0190s0020 57.206 451 173 7 1 440 1 442 0.0 519
MS.gene066656.t1 MTR_4g094835 55.165 455 172 9 1 436 1 442 5.45e-171 487
MS.gene066656.t1 MTR_4g094842 56.044 455 174 8 1 438 1 446 1.44e-168 481
MS.gene066656.t1 MTR_4g094838 51.754 456 169 10 4 436 1 428 2.72e-154 444
MS.gene066656.t1 MTR_4g094828 50.763 459 196 12 1 439 1 449 5.18e-147 426
MS.gene066656.t1 MTR_4g094832 56.283 382 141 6 69 436 57 426 7.33e-146 422
MS.gene066656.t1 MTR_8g079710 51.092 458 191 12 1 440 1 443 1.86e-144 419
MS.gene066656.t1 MTR_6g016815 52.500 440 136 7 1 440 15 381 1.16e-142 412
MS.gene066656.t1 MTR_4g022290 50.763 459 187 13 1 437 1 442 2.16e-140 409
MS.gene066656.t1 MTR_3g101900 38.964 444 243 12 12 440 18 448 3.05e-92 286
MS.gene066656.t1 MTR_8g040760 63.208 212 72 2 1 206 1 212 1.54e-89 271
MS.gene066656.t1 MTR_3g102020 38.409 440 243 10 15 437 20 448 1.38e-88 277
MS.gene066656.t1 MTR_3g101930 37.615 436 241 10 24 439 30 454 8.89e-87 272
MS.gene066656.t1 MTR_3g101970 37.004 454 247 11 3 439 5 436 1.43e-86 271
MS.gene066656.t1 MTR_3g101960 37.529 437 246 9 15 435 20 445 2.65e-86 271
MS.gene066656.t1 MTR_8g040780 59.722 216 83 2 224 435 12 227 1.06e-84 260
MS.gene066656.t1 MTR_3g102010 37.644 433 243 10 24 440 30 451 8.53e-84 265
MS.gene066656.t1 MTR_7g026850 70.060 167 49 1 29 194 12 178 1.50e-81 249
MS.gene066656.t1 MTR_7g026770 59.474 190 48 2 141 329 132 293 1.53e-70 225
MS.gene066656.t1 MTR_3g070170 32.700 474 261 12 3 436 11 466 8.12e-63 210
MS.gene066656.t1 MTR_4g015180 32.784 485 248 11 16 439 19 486 3.50e-61 206
MS.gene066656.t1 MTR_5g081960 31.809 481 251 10 16 436 19 482 5.27e-61 206
MS.gene066656.t1 MTR_6g045553 30.820 451 276 10 16 436 27 471 1.76e-54 188
MS.gene066656.t1 MTR_4g094825 48.000 200 96 5 5 198 72 269 7.55e-54 184
MS.gene066656.t1 MTR_4g094825 57.823 147 49 2 292 436 252 387 9.72e-49 171
MS.gene066656.t1 MTR_3g102030 34.713 314 164 7 87 396 44 320 1.54e-50 174
MS.gene066656.t1 MTR_6g034495 61.194 134 42 3 57 180 31 164 2.16e-48 163
MS.gene066656.t1 MTR_6g034485 57.576 132 48 2 188 319 2 125 1.14e-45 154
MS.gene066656.t1 MTR_7g026800 50.292 171 54 4 211 379 19 160 1.36e-38 138
MS.gene066656.t1 MTR_4g022780 49.133 173 57 4 211 381 20 163 4.02e-38 136
MS.gene066656.t1 MTR_7g026830 71.429 84 24 0 82 165 14 97 1.33e-36 130
MS.gene066656.t1 MTR_7g026780 56.364 110 24 2 331 440 26 111 7.18e-31 115
MS.gene066656.t1 MTR_8g096530 30.312 353 213 12 49 391 53 382 3.59e-30 122
MS.gene066656.t1 MTR_8g075610 27.226 393 242 16 55 429 47 413 1.05e-28 117
MS.gene066656.t1 MTR_4g007540 30.084 359 215 16 55 398 56 393 4.78e-28 115
MS.gene066656.t1 MTR_3g098980 27.704 379 236 13 55 421 66 418 1.74e-27 114
MS.gene066656.t1 MTR_2g064495 27.604 384 241 15 55 422 56 418 1.28e-26 111
MS.gene066656.t1 MTR_2g105330 27.344 384 242 15 55 422 56 418 1.28e-26 111
MS.gene066656.t1 MTR_3g026950 26.562 384 209 16 52 392 51 404 9.54e-23 100
MS.gene066656.t1 MTR_1g081660 26.303 403 226 15 24 392 31 396 1.56e-22 99.8
MS.gene066656.t1 MTR_7g014250 25.280 447 277 16 22 431 24 450 3.91e-21 95.9
MS.gene066656.t1 MTR_4g013050 26.287 369 187 12 53 392 51 363 4.92e-21 95.1
MS.gene066656.t1 MTR_3g071320 25.115 434 291 14 17 436 19 432 8.31e-21 94.7
MS.gene066656.t1 MTR_2g089650 24.638 414 265 18 38 417 43 443 1.92e-20 94.0
MS.gene066656.t1 MTR_7g014330 25.412 425 260 16 18 405 20 424 4.03e-20 92.8
MS.gene066656.t1 MTR_7g026870 74.074 54 14 0 297 350 98 151 9.24e-20 85.9
MS.gene066656.t1 MTR_7g015860 24.029 412 248 14 22 392 26 413 6.38e-19 89.4
MS.gene066656.t1 MTR_7g114520 24.722 360 240 13 44 392 53 392 1.47e-18 88.2
MS.gene066656.t1 MTR_3g086020 26.076 395 243 16 55 440 61 415 1.53e-18 87.8
MS.gene066656.t1 MTR_5g076700 23.832 428 272 16 25 422 24 427 1.96e-18 87.8
MS.gene066656.t1 MTR_3g112180 25.000 444 273 19 9 427 18 426 2.04e-18 87.8
MS.gene066656.t1 MTR_7g114550 23.583 441 284 16 25 436 24 440 3.40e-18 87.0
MS.gene066656.t1 MTR_3g049150 24.823 282 171 8 138 391 131 399 3.64e-18 86.7
MS.gene066656.t1 MTR_7g026880 68.627 51 16 0 389 439 2 52 3.99e-18 78.2
MS.gene066656.t1 MTR_7g014230 25.576 434 248 15 18 405 22 426 1.27e-17 85.1
MS.gene066656.t1 MTR_1g053315 24.048 420 256 18 33 422 60 446 1.39e-17 85.1
MS.gene066656.t1 MTR_4g097090 24.079 407 273 13 55 439 65 457 2.28e-17 84.3
MS.gene066656.t1 MTR_6g015320 25.641 312 202 10 137 427 142 444 4.84e-17 83.6
MS.gene066656.t1 MTR_7g114470 23.898 431 269 18 25 422 25 429 6.43e-17 83.2
MS.gene066656.t1 MTR_7g014200 23.025 443 292 12 22 427 28 458 1.07e-16 82.4
MS.gene066656.t1 MTR_7g014160 25.945 397 254 12 55 421 69 455 1.19e-16 82.4
MS.gene066656.t1 MTR_2g089765 24.757 412 242 14 26 391 45 434 2.42e-16 81.3
MS.gene066656.t1 MTR_7g084940 26.950 282 179 10 53 323 67 332 2.44e-16 81.3
MS.gene066656.t1 MTR_2g089735 25.708 424 255 17 41 424 43 446 2.53e-16 81.3
MS.gene066656.t1 MTR_7g027175 24.870 386 253 14 55 427 68 429 3.51e-16 80.5
MS.gene066656.t1 MTR_2g089670 23.981 417 242 16 21 391 42 429 3.85e-16 80.9
MS.gene066656.t1 MTR_6g015315 24.684 316 207 10 137 430 139 445 4.79e-16 80.5
MS.gene066656.t1 MTR_4g055170 27.815 302 197 11 55 350 69 355 1.06e-15 79.3
MS.gene066656.t1 MTR_6g015830 25.455 440 287 15 17 429 27 452 1.20e-15 79.3
MS.gene066656.t1 MTR_8g012750 23.515 404 270 10 22 391 24 422 1.26e-15 79.3
MS.gene066656.t1 MTR_6g015815 21.719 442 291 14 22 429 32 452 2.45e-15 78.2
MS.gene066656.t1 MTR_7g084910 27.407 270 162 9 53 307 68 318 4.63e-15 77.4
MS.gene066656.t1 MTR_7g085930 26.523 279 186 6 43 308 47 319 7.12e-15 76.6
MS.gene066656.t1 MTR_6g015850 23.611 432 290 12 22 428 32 448 7.89e-15 76.6
MS.gene066656.t1 MTR_5g084870 23.380 432 270 15 5 413 4 397 1.03e-14 76.3
MS.gene066656.t1 MTR_7g014610 25.432 405 249 15 55 421 68 457 2.38e-14 75.1
MS.gene066656.t1 MTR_1g040195 24.645 422 260 18 25 427 23 405 2.55e-14 75.1
MS.gene066656.t1 MTR_6g053810 25.806 279 184 10 55 323 71 336 2.97e-14 74.7
MS.gene066656.t1 MTR_1g040215 23.721 430 260 18 8 408 13 403 8.67e-14 73.6
MS.gene066656.t1 MTR_8g079950 25.606 371 235 14 41 396 42 386 1.32e-13 72.8
MS.gene066656.t1 MTR_5g090810 24.362 431 262 19 2 392 6 412 2.08e-13 72.4
MS.gene066656.t1 MTR_5g090830 24.594 431 261 19 2 392 6 412 8.68e-13 70.5
MS.gene066656.t1 MTR_7g086000 26.442 416 254 18 58 439 63 460 1.43e-12 69.7
MS.gene066656.t1 MTR_7g014360 26.692 266 175 8 138 395 90 343 1.53e-12 69.3
MS.gene066656.t1 MTR_7g085990 26.165 279 187 6 43 308 43 315 2.63e-12 68.9
MS.gene066656.t1 MTR_5g090880 24.728 368 229 16 55 393 67 415 5.22e-12 67.8
MS.gene066656.t1 MTR_3g450710 26.613 372 220 18 52 392 62 411 1.10e-11 67.0
MS.gene066656.t1 MTR_3g450690 26.829 369 220 18 52 392 69 415 1.70e-11 66.2
MS.gene066656.t1 MTR_7g013850 21.831 426 263 16 52 427 65 470 1.77e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066656.t1 AT5G67150 43.349 436 224 11 16 439 21 445 3.25e-112 337
MS.gene066656.t1 AT3G50300 42.069 435 227 11 1 421 1 424 4.25e-100 306
MS.gene066656.t1 AT3G50280 40.371 431 239 8 16 437 19 440 2.23e-98 302
MS.gene066656.t1 AT5G67160 38.657 432 236 10 17 440 22 432 4.51e-93 288
MS.gene066656.t1 AT3G50270 37.969 453 259 10 2 440 4 448 1.08e-91 285
MS.gene066656.t1 AT5G42830 37.123 431 246 10 24 440 30 449 1.49e-84 266
MS.gene066656.t1 AT5G38130 39.379 419 226 12 16 421 39 442 1.81e-84 266
MS.gene066656.t1 AT5G07860 37.701 435 235 10 24 439 31 448 1.85e-80 256
MS.gene066656.t1 AT5G07850 37.071 437 241 12 24 440 31 453 6.82e-75 241
MS.gene066656.t1 AT5G07870 38.061 423 236 10 38 440 45 461 5.66e-74 239
MS.gene066656.t1 AT2G39980 33.473 478 245 13 16 439 21 479 3.30e-68 225
MS.gene066656.t1 AT3G50280 38.871 319 180 5 125 437 1 310 1.88e-66 215
MS.gene066656.t1 AT5G01210 34.542 469 245 13 16 440 19 469 1.52e-64 215
MS.gene066656.t1 AT5G23940 32.751 458 269 13 5 436 4 448 1.69e-64 215
MS.gene066656.t1 AT3G50290 40.876 274 148 7 122 389 4 269 6.78e-51 173
MS.gene066656.t1 AT5G48930 27.989 368 215 10 53 398 54 393 1.32e-28 117
MS.gene066656.t1 AT3G29670 25.117 426 278 14 20 421 25 433 5.53e-25 107
MS.gene066656.t1 AT3G48720 27.473 364 229 12 42 392 44 385 9.77e-25 106
MS.gene066656.t1 AT3G29590 26.281 449 298 17 3 431 7 442 1.23e-23 103
MS.gene066656.t1 AT2G19070 25.054 459 271 21 27 438 19 451 1.05e-22 100
MS.gene066656.t1 AT5G41040 28.011 357 218 16 50 392 64 395 3.68e-22 98.6
MS.gene066656.t1 AT5G41040 28.011 357 218 16 50 392 80 411 4.32e-22 98.6
MS.gene066656.t1 AT5G57840 27.873 409 242 18 41 421 39 422 5.34e-20 92.4
MS.gene066656.t1 AT5G39080 25.990 404 245 18 52 421 62 445 7.19e-20 92.0
MS.gene066656.t1 AT3G29635 24.771 436 268 16 22 421 27 438 3.43e-19 90.1
MS.gene066656.t1 AT5G63560 26.735 389 238 16 50 422 56 413 4.74e-19 89.4
MS.gene066656.t1 AT3G29680 25.879 398 248 15 49 421 56 431 6.65e-19 89.0
MS.gene066656.t1 AT1G24430 24.654 434 262 19 26 440 38 425 1.46e-18 87.8
MS.gene066656.t1 AT5G39050 24.282 383 226 13 66 414 90 442 3.42e-18 87.0
MS.gene066656.t1 AT3G26040 24.272 412 257 17 49 440 53 429 5.46e-18 86.3
MS.gene066656.t1 AT2G40230 24.645 422 287 16 22 431 24 426 1.19e-17 85.1
MS.gene066656.t1 AT1G28680 25.126 398 248 15 58 440 65 427 1.91e-16 81.6
MS.gene066656.t1 AT5G07080 27.027 296 183 13 42 321 53 331 5.01e-16 80.5
MS.gene066656.t1 AT1G03390 25.490 408 255 19 42 422 59 444 1.54e-15 79.0
MS.gene066656.t1 AT5G39090 26.642 274 170 12 135 391 140 399 1.91e-15 78.6
MS.gene066656.t1 AT5G61160 21.554 399 267 14 22 392 23 403 8.49e-15 76.6
MS.gene066656.t1 AT5G17540 25.792 442 269 21 25 434 23 437 4.13e-14 74.3
MS.gene066656.t1 AT5G23970 26.667 450 278 21 4 436 3 417 5.14e-14 73.9
MS.gene066656.t1 AT1G28680 24.916 297 202 9 58 348 65 346 5.73e-14 73.6
MS.gene066656.t1 AT3G30280 24.000 375 211 17 54 398 61 391 6.67e-14 73.9
MS.gene066656.t1 AT5G16410 22.558 430 286 15 1 413 45 444 3.53e-13 71.6
MS.gene066656.t1 AT1G65450 25.352 284 188 12 53 324 70 341 7.12e-13 70.5
MS.gene066656.t1 AT1G03940 23.020 404 277 11 50 428 61 455 9.05e-13 70.5
MS.gene066656.t1 AT1G32910 25.876 371 210 18 53 392 63 399 9.85e-13 70.1
MS.gene066656.t1 AT2G23510 24.611 386 255 18 55 422 69 436 1.04e-12 70.1
MS.gene066656.t1 AT3G47170 25.839 298 184 11 44 321 64 344 2.42e-12 68.9
MS.gene066656.t1 AT2G25150 25.414 362 234 14 50 395 62 403 7.25e-12 67.4
MS.gene066656.t1 AT4G15390 22.525 404 258 15 10 394 24 391 2.53e-11 65.9
MS.gene066656.t1 AT4G15390 22.525 404 258 15 10 394 24 391 2.53e-11 65.9

Find 82 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCATGGTTTGATGTTTA+TGG 0.113342 4.2:-80053023 MS.gene066656:CDS
GTGTCTTCCTTACAAGATTT+TGG 0.202537 4.2:-80052892 MS.gene066656:CDS
CCATTGCCAGAAGATTATTT+TGG 0.248348 4.2:-80053254 MS.gene066656:CDS
GATGTTTATGGTAATGATTT+TGG 0.308658 4.2:-80053011 MS.gene066656:CDS
TGGTTGGATAAAGTAGAAAT+TGG 0.327409 4.2:+80054062 None:intergenic
GCGCTTAATACGTTCATAAT+AGG 0.328958 4.2:-80053299 MS.gene066656:CDS
TGATCATGAATTCATGGATA+TGG 0.332225 4.2:-80052856 MS.gene066656:CDS
AATGTCTTAGCCGTTGAATT+TGG 0.349561 4.2:+80053999 None:intergenic
TTCTTGTGGATCAAGATGTT+TGG 0.349905 4.2:+80053325 None:intergenic
AATGAAAGCTGGGGAGTTGT+TGG 0.360707 4.2:-80053204 MS.gene066656:CDS
GAAACCAACGCTCTAGTGTT+GGG 0.360883 4.2:+80053588 None:intergenic
TTTCATTGCTTTGATTGTAT+TGG 0.364692 4.2:+80054123 None:intergenic
GGAATGGTTTGTGCCTTTGC+TGG 0.368156 4.2:-80052945 MS.gene066656:CDS
TGAGAAATCCAAGCTTCATT+AGG 0.373854 4.2:-80053088 MS.gene066656:CDS
GCGTTTCCAAAATAATCTTC+TGG 0.379778 4.2:+80053248 None:intergenic
GCCAGCAAGAGGTTCGAAAA+AGG 0.381613 4.2:+80053958 None:intergenic
CTAGCTGACGGCATCTTCAT+AGG 0.398680 4.2:-80053713 MS.gene066656:CDS
GGGAGTTGTTGGAGGAAGGT+GGG 0.400037 4.2:-80053193 MS.gene066656:CDS
GAAACGCGATTATAGGTTGC+GGG 0.410719 4.2:-80053232 MS.gene066656:CDS
ATGGGCTGTTGAATACCATT+TGG 0.419783 4.2:+80053566 None:intergenic
TTGTATTGGTGGAGAGTACT+TGG 0.455345 4.2:+80054137 None:intergenic
AAACCATGGAGAACTGCTAA+TGG 0.455624 4.2:+80053034 None:intergenic
GGGAAATGATCATGAATTCA+TGG 0.457595 4.2:-80052862 MS.gene066656:CDS
TTGTTGGAGGAAGGTGGGAT+TGG 0.457605 4.2:-80053188 MS.gene066656:CDS
GGGGAGTTGTTGGAGGAAGG+TGG 0.459892 4.2:-80053194 MS.gene066656:CDS
CGGAGTGGAAATAGAACAAA+TGG 0.464324 4.2:-80052966 MS.gene066656:CDS
TGCGGGTTTAAAATGAAAGC+TGG 0.465815 4.2:-80053215 MS.gene066656:CDS
GGCAATGGTGGAACAAGTCT+TGG 0.472119 4.2:+80053269 None:intergenic
CCAAAATAATCTTCTGGCAA+TGG 0.477773 4.2:+80053254 None:intergenic
GCTGGGGAGTTGTTGGAGGA+AGG 0.483907 4.2:-80053197 MS.gene066656:CDS
AAAACCCACATATGTTCCTT+CGG 0.485243 4.2:-80053819 MS.gene066656:CDS
GAGGAAGGTGGGATTGGTAA+AGG 0.486075 4.2:-80053182 MS.gene066656:CDS
CATTGCTTTGATTGTATTGG+TGG 0.486507 4.2:+80054126 None:intergenic
CTATCAAGCCTAATGAAGCT+TGG 0.491157 4.2:+80053080 None:intergenic
GGGGAAAGCCTGTGGCAGTT+CGG 0.491671 4.2:-80052986 MS.gene066656:CDS
ATAAAATTGATTTAACTCCA+TGG 0.501405 4.2:-80054090 MS.gene066656:CDS
ATTGCCTCCAAAATCTTGTA+AGG 0.504306 4.2:+80052885 None:intergenic
TAAAATTGATTTAACTCCAT+GGG 0.505681 4.2:-80054089 MS.gene066656:CDS
GGAAACCAACGCTCTAGTGT+TGG 0.511281 4.2:+80053587 None:intergenic
GCCTTTGCTGGAATAGAAGA+AGG 0.511653 4.2:-80052933 MS.gene066656:CDS
TTCAATCATGTTGTAGCTGA+CGG 0.511810 4.2:-80053683 MS.gene066656:CDS
TATAGTTAAAGGAAATCGTA+TGG 0.515144 4.2:+80053547 None:intergenic
CTAGAGCGTTGGTTTCCAAA+TGG 0.515960 4.2:-80053581 MS.gene066656:CDS
TTTCCACTCCGAACTGCCAC+AGG 0.522499 4.2:+80052978 None:intergenic
TGAATTTGGTCTGATGCTAC+AGG 0.526430 4.2:+80054013 None:intergenic
TGTAAGGAAGACACACTTGA+AGG 0.538009 4.2:+80052901 None:intergenic
TAAAGAATGATTATGAGTCA+TGG 0.538860 4.2:-80053112 MS.gene066656:CDS
GCGGGTTTAAAATGAAAGCT+GGG 0.538973 4.2:-80053214 MS.gene066656:CDS
GTATTAAGCGCATCTTCTTG+TGG 0.540410 4.2:+80053311 None:intergenic
AGGTACATCAAAATCATTGT+TGG 0.540953 4.2:-80053753 MS.gene066656:CDS
GAAAGCTGGGGAGTTGTTGG+AGG 0.541663 4.2:-80053201 MS.gene066656:CDS
TGGCCATTAGCAGTTCTCCA+TGG 0.541677 4.2:-80053037 MS.gene066656:CDS
AAATAATCTTCTGGCAATGG+TGG 0.542517 4.2:+80053257 None:intergenic
GGAAACGCGATTATAGGTTG+CGG 0.544739 4.2:-80053233 MS.gene066656:CDS
TAGTTTGTAACTCCGTTGAA+AGG 0.548129 4.2:+80053779 None:intergenic
CGTGTTCTGTAACTTTGAGA+CGG 0.548497 4.2:+80053936 None:intergenic
TGGAAATAGAACAAATGGAA+TGG 0.553380 4.2:-80052961 MS.gene066656:CDS
ACCTTCTTCTATTCCAGCAA+AGG 0.555908 4.2:+80052932 None:intergenic
AGTAGAAATTGGAGATCCCA+TGG 0.565431 4.2:+80054073 None:intergenic
AAACCAACGCTCTAGTGTTG+GGG 0.569521 4.2:+80053589 None:intergenic
ATGGCATTTCATCAATTCGT+GGG 0.570140 4.2:-80053651 MS.gene066656:CDS
AACAACCGAAGGAACATATG+TGG 0.575430 4.2:+80053814 None:intergenic
ATAGTTAAAGGAAATCGTAT+GGG 0.585010 4.2:+80053548 None:intergenic
CTACAGGATGGTGATAAAGA+AGG 0.592406 4.2:+80054029 None:intergenic
CATGGCATTTCATCAATTCG+TGG 0.610417 4.2:-80053652 MS.gene066656:CDS
ACTTTGAGACGGCCAGCAAG+AGG 0.616523 4.2:+80053947 None:intergenic
TCTGTAACATGCAATAATGC+AGG 0.619827 4.2:-80053893 MS.gene066656:CDS
ATTGTTGGCAGTTCAAGTGA+CGG 0.619862 4.2:-80053738 MS.gene066656:CDS
AACCAACGCTCTAGTGTTGG+GGG 0.632370 4.2:+80053590 None:intergenic
TAGCTGACGGACAGTCCACA+TGG 0.634434 4.2:-80053670 MS.gene066656:CDS
CAAGTGACGGAGCTAGCTGA+CGG 0.639821 4.2:-80053725 MS.gene066656:CDS
CGGGTTTAAAATGAAAGCTG+GGG 0.642028 4.2:-80053213 MS.gene066656:CDS
AAGCCTGTGGCAGTTCGGAG+TGG 0.647498 4.2:-80052981 MS.gene066656:CDS
ACAACCGAAGGAACATATGT+GGG 0.650889 4.2:+80053815 None:intergenic
AACGGAGTTACAAACTACGA+AGG 0.656439 4.2:-80053773 MS.gene066656:CDS
TTACCATAAACATCAAACCA+TGG 0.659822 4.2:+80053020 None:intergenic
TAGCATCAGACCAAATTCAA+CGG 0.663707 4.2:-80054009 MS.gene066656:CDS
AGGTGTAAACTTTATACATG+CGG 0.677512 4.2:-80053873 MS.gene066656:CDS
GCCACAAAAGAATTACTCTG+AGG 0.711684 4.2:-80053522 MS.gene066656:CDS
CGAATTGATGAAATGCCATG+TGG 0.718082 4.2:+80053655 None:intergenic
TTTGGTCTGATGCTACAGGA+TGG 0.756053 4.2:+80054017 None:intergenic
TCGTGGGCTGAAATGTCACG+TGG 0.758657 4.2:-80053635 MS.gene066656:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAAATTGATTTAACTCCA+TGG - chr4.2:80052906-80052925 MS.gene066656:CDS 20.0%
!!! TAAAATTGATTTAACTCCAT+GGG - chr4.2:80052907-80052926 MS.gene066656:CDS 20.0%
!!! TTGTAGAGAAGATATTTTTT+TGG + chr4.2:80053614-80053633 None:intergenic 20.0%
! AAAAATTCTATCTATCTGAG+AGG - chr4.2:80053524-80053543 MS.gene066656:CDS 25.0%
! ATAGTTAAAGGAAATCGTAT+GGG + chr4.2:80053451-80053470 None:intergenic 25.0%
! TAAAGAATGATTATGAGTCA+TGG - chr4.2:80053884-80053903 MS.gene066656:CDS 25.0%
! TATAGTTAAAGGAAATCGTA+TGG + chr4.2:80053452-80053471 None:intergenic 25.0%
! TTTCATTGCTTTGATTGTAT+TGG + chr4.2:80052876-80052895 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG - chr4.2:80053985-80054004 MS.gene066656:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG - chr4.2:80053990-80054009 MS.gene066656:CDS 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG - chr4.2:80053989-80054008 MS.gene066656:CDS 25.0%
AGGTACATCAAAATCATTGT+TGG - chr4.2:80053243-80053262 MS.gene066656:CDS 30.0%
AGGTGTAAACTTTATACATG+CGG - chr4.2:80053123-80053142 MS.gene066656:CDS 30.0%
CTTTATCCAACCAACAAAAA+AGG - chr4.2:80052944-80052963 MS.gene066656:CDS 30.0%
TGATCATGAATTCATGGATA+TGG - chr4.2:80054140-80054159 MS.gene066656:CDS 30.0%
TGGAAATAGAACAAATGGAA+TGG - chr4.2:80054035-80054054 MS.gene066656:CDS 30.0%
TGGTTGGATAAAGTAGAAAT+TGG + chr4.2:80052937-80052956 None:intergenic 30.0%
TTACCATAAACATCAAACCA+TGG + chr4.2:80053979-80053998 None:intergenic 30.0%
!! TATGGTAATGATTTTGGATG+GGG - chr4.2:80053991-80054010 MS.gene066656:CDS 30.0%
!! TATTTTGGAAACGCGATTAT+AGG - chr4.2:80053757-80053776 MS.gene066656:CDS 30.0%
!! TTTTGTGGCTCTATAGTTAA+AGG + chr4.2:80053463-80053482 None:intergenic 30.0%
!!! ATAAAGAAGGCCTTTTTTGT+TGG + chr4.2:80052957-80052976 None:intergenic 30.0%
!!! CGTTCATTTTTTCCTTTCAA+CGG - chr4.2:80053205-80053224 MS.gene066656:CDS 30.0%
!!! CTTGAGCACTAAGAATTTTT+TGG - chr4.2:80053939-80053958 MS.gene066656:CDS 30.0%
AAAAATGAACGAACAACCGA+AGG + chr4.2:80053196-80053215 None:intergenic 35.0%
AAAACCCACATATGTTCCTT+CGG - chr4.2:80053177-80053196 MS.gene066656:CDS 35.0%
AAATAATCTTCTGGCAATGG+TGG + chr4.2:80053742-80053761 None:intergenic 35.0%
AAGAGGTTCGAAAAAGGAAA+GGG + chr4.2:80053035-80053054 None:intergenic 35.0%
AATGTCTTAGCCGTTGAATT+TGG + chr4.2:80053000-80053019 None:intergenic 35.0%
ATGGCATTTCATCAATTCGT+GGG - chr4.2:80053345-80053364 MS.gene066656:CDS 35.0%
ATTGCCTCCAAAATCTTGTA+AGG + chr4.2:80054114-80054133 None:intergenic 35.0%
CATTGCTTTGATTGTATTGG+TGG + chr4.2:80052873-80052892 None:intergenic 35.0%
CCAAAATAATCTTCTGGCAA+TGG + chr4.2:80053745-80053764 None:intergenic 35.0%
GCGCTTAATACGTTCATAAT+AGG - chr4.2:80053697-80053716 MS.gene066656:CDS 35.0%
GCGTTTCCAAAATAATCTTC+TGG + chr4.2:80053751-80053770 None:intergenic 35.0%
GGGAAATGATCATGAATTCA+TGG - chr4.2:80054134-80054153 MS.gene066656:CDS 35.0%
TAGCATCAGACCAAATTCAA+CGG - chr4.2:80052987-80053006 MS.gene066656:CDS 35.0%
TCTCCATGGTTTGATGTTTA+TGG - chr4.2:80053973-80053992 MS.gene066656:CDS 35.0%
TCTGTAACATGCAATAATGC+AGG - chr4.2:80053103-80053122 MS.gene066656:CDS 35.0%
TGAGAAATCCAAGCTTCATT+AGG - chr4.2:80053908-80053927 MS.gene066656:CDS 35.0%
TTCAATCATGTTGTAGCTGA+CGG - chr4.2:80053313-80053332 MS.gene066656:CDS 35.0%
TTCTTGTGGATCAAGATGTT+TGG + chr4.2:80053674-80053693 None:intergenic 35.0%
! AAGGAACATATGTGGGTTTT+AGG + chr4.2:80053177-80053196 None:intergenic 35.0%
! CCATTGCCAGAAGATTATTT+TGG - chr4.2:80053742-80053761 MS.gene066656:CDS 35.0%
! GTGTCTTCCTTACAAGATTT+TGG - chr4.2:80054104-80054123 MS.gene066656:CDS 35.0%
! TAGTTTGTAACTCCGTTGAA+AGG + chr4.2:80053220-80053239 None:intergenic 35.0%
! TCCTCAGAGTAATTCTTTTG+TGG + chr4.2:80053478-80053497 None:intergenic 35.0%
! TCTTCCTTACAAGATTTTGG+AGG - chr4.2:80054107-80054126 MS.gene066656:CDS 35.0%
!! AAGCGCTTTTAACTCATCTT+TGG - chr4.2:80053632-80053651 MS.gene066656:CDS 35.0%
!! TACAAGATTTTGGAGGCAAT+GGG - chr4.2:80054114-80054133 MS.gene066656:CDS 35.0%
!! TTACAAGATTTTGGAGGCAA+TGG - chr4.2:80054113-80054132 MS.gene066656:CDS 35.0%
AAACCATGGAGAACTGCTAA+TGG + chr4.2:80053965-80053984 None:intergenic 40.0%
AACAACCGAAGGAACATATG+TGG + chr4.2:80053185-80053204 None:intergenic 40.0%
AACGGAGTTACAAACTACGA+AGG - chr4.2:80053223-80053242 MS.gene066656:CDS 40.0%
ACAACCGAAGGAACATATGT+GGG + chr4.2:80053184-80053203 None:intergenic 40.0%
ACCTTCTTCTATTCCAGCAA+AGG + chr4.2:80054067-80054086 None:intergenic 40.0%
AGTAGAAATTGGAGATCCCA+TGG + chr4.2:80052926-80052945 None:intergenic 40.0%
ATTGTTGGCAGTTCAAGTGA+CGG - chr4.2:80053258-80053277 MS.gene066656:CDS 40.0%
CAAGAGGTTCGAAAAAGGAA+AGG + chr4.2:80053036-80053055 None:intergenic 40.0%
CATGGCATTTCATCAATTCG+TGG - chr4.2:80053344-80053363 MS.gene066656:CDS 40.0%
CGAATTGATGAAATGCCATG+TGG + chr4.2:80053344-80053363 None:intergenic 40.0%
CGGAGTGGAAATAGAACAAA+TGG - chr4.2:80054030-80054049 MS.gene066656:CDS 40.0%
CTACAGGATGGTGATAAAGA+AGG + chr4.2:80052970-80052989 None:intergenic 40.0%
CTATCAAGCCTAATGAAGCT+TGG + chr4.2:80053919-80053938 None:intergenic 40.0%
GCCACAAAAGAATTACTCTG+AGG - chr4.2:80053474-80053493 MS.gene066656:CDS 40.0%
GTATTAAGCGCATCTTCTTG+TGG + chr4.2:80053688-80053707 None:intergenic 40.0%
TGAATTTGGTCTGATGCTAC+AGG + chr4.2:80052986-80053005 None:intergenic 40.0%
TGTAAGGAAGACACACTTGA+AGG + chr4.2:80054098-80054117 None:intergenic 40.0%
! ATGGGCTGTTGAATACCATT+TGG + chr4.2:80053433-80053452 None:intergenic 40.0%
! CGGGTTTAAAATGAAAGCTG+GGG - chr4.2:80053783-80053802 MS.gene066656:CDS 40.0%
! CGTGTTCTGTAACTTTGAGA+CGG + chr4.2:80053063-80053082 None:intergenic 40.0%
! GCGGGTTTAAAATGAAAGCT+GGG - chr4.2:80053782-80053801 MS.gene066656:CDS 40.0%
! TGCGGGTTTAAAATGAAAGC+TGG - chr4.2:80053781-80053800 MS.gene066656:CDS 40.0%
!! TTGTATTGGTGGAGAGTACT+TGG + chr4.2:80052862-80052881 None:intergenic 40.0%
!!! AGAAGGCCTTTTTTGTTGGT+TGG + chr4.2:80052953-80052972 None:intergenic 40.0%
AAACCAACGCTCTAGTGTTG+GGG + chr4.2:80053410-80053429 None:intergenic 45.0%
AATGAAAGCTGGGGAGTTGT+TGG - chr4.2:80053792-80053811 MS.gene066656:CDS 45.0%
GAAACCAACGCTCTAGTGTT+GGG + chr4.2:80053411-80053430 None:intergenic 45.0%
GAAACGCGATTATAGGTTGC+GGG - chr4.2:80053764-80053783 MS.gene066656:CDS 45.0%
GCCTTTGCTGGAATAGAAGA+AGG - chr4.2:80054063-80054082 MS.gene066656:CDS 45.0%
GGAAACGCGATTATAGGTTG+CGG - chr4.2:80053763-80053782 MS.gene066656:CDS 45.0%
TTTGGTCTGATGCTACAGGA+TGG + chr4.2:80052982-80053001 None:intergenic 45.0%
!! CTAGAGCGTTGGTTTCCAAA+TGG - chr4.2:80053415-80053434 MS.gene066656:CDS 45.0%
!!! TCCTTTTTCGAACCTCTTGC+TGG - chr4.2:80053037-80053056 MS.gene066656:CDS 45.0%
CTAGCTGACGGCATCTTCAT+AGG - chr4.2:80053283-80053302 MS.gene066656:CDS 50.0%
GCCAGCAAGAGGTTCGAAAA+AGG + chr4.2:80053041-80053060 None:intergenic 50.0%
GGAAACCAACGCTCTAGTGT+TGG + chr4.2:80053412-80053431 None:intergenic 50.0%
TGGCCATTAGCAGTTCTCCA+TGG - chr4.2:80053959-80053978 MS.gene066656:CDS 50.0%
TTGTTGGAGGAAGGTGGGAT+TGG - chr4.2:80053808-80053827 MS.gene066656:CDS 50.0%
! AACCAACGCTCTAGTGTTGG+GGG + chr4.2:80053409-80053428 None:intergenic 50.0%
! GAGGAAGGTGGGATTGGTAA+AGG - chr4.2:80053814-80053833 MS.gene066656:CDS 50.0%
! GGAATGGTTTGTGCCTTTGC+TGG - chr4.2:80054051-80054070 MS.gene066656:CDS 50.0%
! GGCAATGGTGGAACAAGTCT+TGG + chr4.2:80053730-80053749 None:intergenic 50.0%
! TTTTGGATGGGGAAAGCCTG+TGG - chr4.2:80054002-80054021 MS.gene066656:CDS 50.0%
AACCCCCAACACTAGAGCGT+TGG - chr4.2:80053404-80053423 MS.gene066656:CDS 55.0%
GAAAGCTGGGGAGTTGTTGG+AGG - chr4.2:80053795-80053814 MS.gene066656:CDS 55.0%
GGGAGTTGTTGGAGGAAGGT+GGG - chr4.2:80053803-80053822 MS.gene066656:CDS 55.0%
TTTCCACTCCGAACTGCCAC+AGG + chr4.2:80054021-80054040 None:intergenic 55.0%
! CAAGTGACGGAGCTAGCTGA+CGG - chr4.2:80053271-80053290 MS.gene066656:CDS 55.0%
! TAGCTGACGGACAGTCCACA+TGG - chr4.2:80053326-80053345 MS.gene066656:CDS 55.0%
! TCGTGGGCTGAAATGTCACG+TGG - chr4.2:80053361-80053380 MS.gene066656:CDS 55.0%
!! ACTTTGAGACGGCCAGCAAG+AGG + chr4.2:80053052-80053071 None:intergenic 55.0%
AAGCCTGTGGCAGTTCGGAG+TGG - chr4.2:80054015-80054034 MS.gene066656:CDS 60.0%
GCTGGGGAGTTGTTGGAGGA+AGG - chr4.2:80053799-80053818 MS.gene066656:CDS 60.0%
GGGGAAAGCCTGTGGCAGTT+CGG - chr4.2:80054010-80054029 MS.gene066656:CDS 60.0%
GGGGAGTTGTTGGAGGAAGG+TGG - chr4.2:80053802-80053821 MS.gene066656:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 80052848 80054170 80052848 ID=MS.gene066656
chr4.2 mRNA 80052848 80054170 80052848 ID=MS.gene066656.t1;Parent=MS.gene066656
chr4.2 exon 80052848 80054170 80052848 ID=MS.gene066656.t1.exon1;Parent=MS.gene066656.t1
chr4.2 CDS 80052848 80054170 80052848 ID=cds.MS.gene066656.t1;Parent=MS.gene066656.t1
Gene Sequence

>MS.gene066656

ATGGGTGCATTCCAAGTACTCTCCACCAATACAATCAAAGCAATGAAATCAAGTAATCATAAAATTGATTTAACTCCATGGGATCTCCAATTTCTACTTTATCCAACCAACAAAAAAGGCCTTCTTTATCACCATCCTGTAGCATCAGACCAAATTCAACGGCTAAGACATTCTCTTTCTTCTGCCCTTTCCTTTTTCGAACCTCTTGCTGGCCGTCTCAAAGTTACAGAACACGAAGACAGTACTGTCTCGTGTTCTGTAACATGCAATAATGCAGGTGTAAACTTTATACATGCGGCAGCAAAGAATACTTGTGTTGCTGACATCCTAAAACCCACATATGTTCCTTCGGTTGTTCGTTCATTTTTTCCTTTCAACGGAGTTACAAACTACGAAGGTACATCAAAATCATTGTTGGCAGTTCAAGTGACGGAGCTAGCTGACGGCATCTTCATAGGCTGTAATTTCAATCATGTTGTAGCTGACGGACAGTCCACATGGCATTTCATCAATTCGTGGGCTGAAATGTCACGTGGTTGTCATCATCAAATATCTAAACCCCCAACACTAGAGCGTTGGTTTCCAAATGGTATTCAACAGCCCATACGATTTCCTTTAACTATAGAGCCACAAAAGAATTACTCTGAGGAGTTGAGTTTTTCGTCATCTGATGACGAAAAATTCTATCTATCTGAGAGGATATTTCACTTCACAAAGAAGAAAATCATGCAATTGAAATCAAAAGTCAACGCAGCGATTGATACCAAAAAAATATCTTCTCTACAAGCGCTTTTAACTCATCTTTGGTGTTCAGTGATTCGTTCCAAACATCTTGATCCACAAGAAGATGCGCTTAATACGTTCATAATAGGAGTTAGACCAAGACTTGTTCCACCATTGCCAGAAGATTATTTTGGAAACGCGATTATAGGTTGCGGGTTTAAAATGAAAGCTGGGGAGTTGTTGGAGGAAGGTGGGATTGGTAAAGGTGCTTTGTTGATGAATAAGTTAATTGCTTTGCACTCTAATGAGAAATTAAAGAATGATTATGAGTCATGGTTGAGAAATCCAAGCTTCATTAGGCTTGATAGCTTGAGCACTAAGAATTTTTTGGCCATTAGCAGTTCTCCATGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAGCCTGTGGCAGTTCGGAGTGGAAATAGAACAAATGGAATGGTTTGTGCCTTTGCTGGAATAGAAGAAGGTAGCATTGACCTTCAAGTGTGTCTTCCTTACAAGATTTTGGAGGCAATGGGAAATGATCATGAATTCATGGATATGGTTAACTAA
Protein sequence

>MS.gene066656.t1

MGAFQVLSTNTIKAMKSSNHKIDLTPWDLQFLLYPTNKKGLLYHHPVASDQIQRLRHSLSSALSFFEPLAGRLKVTEHEDSTVSCSVTCNNAGVNFIHAAAKNTCVADILKPTYVPSVVRSFFPFNGVTNYEGTSKSLLAVQVTELADGIFIGCNFNHVVADGQSTWHFINSWAEMSRGCHHQISKPPTLERWFPNGIQQPIRFPLTIEPQKNYSEELSFSSSDDEKFYLSERIFHFTKKKIMQLKSKVNAAIDTKKISSLQALLTHLWCSVIRSKHLDPQEDALNTFIIGVRPRLVPPLPEDYFGNAIIGCGFKMKAGELLEEGGIGKGALLMNKLIALHSNEKLKNDYESWLRNPSFIRLDSLSTKNFLAISSSPWFDVYGNDFGWGKPVAVRSGNRTNGMVCAFAGIEEGSIDLQVCLPYKILEAMGNDHEFMDMVN