AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99218

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99218.t1 MTR_4g094835 70.549 455 123 3 1 453 1 446 0.0 667
MS.gene99218.t1 MTR_4g094838 71.116 457 102 7 4 454 1 433 0.0 625
MS.gene99218.t1 MTR_4g094832 72.414 377 96 3 81 454 60 431 0.0 551
MS.gene99218.t1 MTR_4g094842 59.692 454 168 7 1 449 1 444 0.0 537
MS.gene99218.t1 MTR_0190s0020 57.965 452 173 8 1 449 1 438 0.0 519
MS.gene99218.t1 MTR_8g041770 56.195 452 180 8 1 449 1 437 3.65e-178 506
MS.gene99218.t1 MTR_4g094828 55.604 455 179 10 1 445 1 442 1.87e-174 496
MS.gene99218.t1 MTR_4g022720 55.973 452 175 8 1 449 1 431 2.07e-174 496
MS.gene99218.t1 MTR_3g088420 56.984 451 170 10 1 449 1 429 3.63e-174 495
MS.gene99218.t1 MTR_4g022825 55.142 457 176 10 1 449 1 436 5.22e-172 490
MS.gene99218.t1 MTR_8g079710 56.512 453 171 10 1 444 1 436 1.55e-171 489
MS.gene99218.t1 MTR_4g022800 54.902 459 176 10 1 449 1 438 3.62e-171 488
MS.gene99218.t1 MTR_4g022290 56.484 455 170 12 1 445 1 437 1.07e-169 484
MS.gene99218.t1 MTR_4g022670 53.712 458 182 10 1 449 1 437 4.11e-166 475
MS.gene99218.t1 MTR_3g026310 53.233 464 182 11 1 449 1 444 6.15e-165 472
MS.gene99218.t1 MTR_7g026860 53.829 457 180 11 1 449 1 434 1.55e-161 463
MS.gene99218.t1 MTR_4g022760 48.578 457 163 10 1 449 1 393 5.47e-139 404
MS.gene99218.t1 MTR_6g016815 42.163 453 168 14 1 449 15 377 7.82e-103 311
MS.gene99218.t1 MTR_4g094825 84.571 175 23 2 20 194 80 250 1.59e-93 288
MS.gene99218.t1 MTR_4g094825 84.516 155 14 1 300 454 248 392 8.46e-85 266
MS.gene99218.t1 MTR_3g101900 39.675 431 239 9 28 449 26 444 5.56e-93 288
MS.gene99218.t1 MTR_3g102020 37.931 464 250 11 3 449 5 447 6.00e-91 283
MS.gene99218.t1 MTR_3g101930 37.986 437 237 9 32 449 30 451 2.50e-88 277
MS.gene99218.t1 MTR_3g102010 37.879 462 250 11 4 449 7 447 1.66e-87 275
MS.gene99218.t1 MTR_3g101960 37.093 461 255 10 3 448 5 445 2.94e-86 271
MS.gene99218.t1 MTR_3g101970 37.150 428 236 8 32 449 29 433 1.04e-83 264
MS.gene99218.t1 MTR_5g081960 33.264 484 243 9 30 454 25 487 3.93e-79 254
MS.gene99218.t1 MTR_3g070170 35.421 463 237 10 30 453 31 470 1.76e-78 252
MS.gene99218.t1 MTR_8g040780 57.488 207 83 3 242 445 20 224 2.72e-75 236
MS.gene99218.t1 MTR_8g040760 54.185 227 89 5 1 224 1 215 1.21e-74 233
MS.gene99218.t1 MTR_4g015180 32.444 487 245 11 27 449 17 483 2.86e-69 228
MS.gene99218.t1 MTR_7g026850 54.286 175 70 3 37 209 12 178 1.72e-57 187
MS.gene99218.t1 MTR_6g045553 28.333 480 296 10 2 450 10 472 2.48e-49 175
MS.gene99218.t1 MTR_3g102030 33.007 306 168 4 106 407 48 320 7.15e-48 167
MS.gene99218.t1 MTR_7g026770 49.171 181 65 4 157 335 132 287 7.38e-46 161
MS.gene99218.t1 MTR_6g034495 58.594 128 44 2 72 194 37 160 9.36e-44 151
MS.gene99218.t1 MTR_6g034485 53.846 130 55 2 202 331 1 125 1.03e-42 147
MS.gene99218.t1 MTR_7g026830 68.293 82 26 0 100 181 16 97 3.62e-33 121
MS.gene99218.t1 MTR_4g007540 25.888 394 254 12 64 442 56 426 8.04e-28 115
MS.gene99218.t1 MTR_8g096530 29.201 363 214 13 57 405 52 385 2.01e-26 111
MS.gene99218.t1 MTR_3g098980 25.874 429 259 18 37 435 20 419 7.19e-26 109
MS.gene99218.t1 MTR_3g026950 24.450 409 256 15 61 438 51 437 9.65e-25 106
MS.gene99218.t1 MTR_2g105330 23.869 398 259 10 64 438 56 432 1.11e-24 106
MS.gene99218.t1 MTR_3g049150 24.515 412 267 11 56 440 43 437 2.25e-24 105
MS.gene99218.t1 MTR_2g064495 23.499 383 260 9 64 434 56 417 1.24e-23 103
MS.gene99218.t1 MTR_4g097090 24.814 403 244 13 64 434 65 440 3.83e-23 102
MS.gene99218.t1 MTR_8g075610 24.433 397 252 15 64 442 47 413 1.31e-22 100
MS.gene99218.t1 MTR_1g081660 23.529 442 297 15 32 453 31 451 2.02e-21 96.7
MS.gene99218.t1 MTR_7g027175 26.131 398 261 12 52 440 56 429 3.17e-21 95.9
MS.gene99218.t1 MTR_4g127650 22.348 443 291 14 22 434 21 440 5.55e-21 95.5
MS.gene99218.t1 MTR_3g071320 26.488 336 203 12 49 362 42 355 7.36e-21 94.7
MS.gene99218.t1 MTR_7g026780 46.296 108 32 3 342 449 26 107 1.20e-20 87.4
MS.gene99218.t1 MTR_6g015315 26.019 319 201 7 155 442 141 455 5.54e-19 89.4
MS.gene99218.t1 MTR_6g015850 24.307 469 302 15 2 442 6 449 6.23e-19 89.4
MS.gene99218.t1 MTR_3g087640 26.201 458 281 19 30 447 29 469 8.24e-19 89.0
MS.gene99218.t1 MTR_7g026800 37.654 162 69 6 231 390 29 160 1.41e-18 84.0
MS.gene99218.t1 MTR_7g084940 26.027 365 227 12 52 402 57 392 2.98e-18 87.0
MS.gene99218.t1 MTR_2g089765 24.583 480 290 20 3 437 15 467 4.78e-18 86.7
MS.gene99218.t1 MTR_4g022780 36.364 165 73 6 231 393 30 164 5.57e-18 82.0
MS.gene99218.t1 MTR_6g015815 24.130 431 282 15 37 442 42 452 6.44e-18 86.3
MS.gene99218.t1 MTR_7g014330 25.061 411 245 16 30 402 24 409 6.84e-18 86.3
MS.gene99218.t1 MTR_5g084870 25.628 398 231 17 33 403 17 376 9.51e-18 85.5
MS.gene99218.t1 MTR_8g012750 25.647 425 238 18 30 402 24 422 1.85e-17 85.1
MS.gene99218.t1 MTR_6g015320 26.667 315 188 12 155 440 144 444 1.90e-17 84.7
MS.gene99218.t1 MTR_3g049140 26.160 237 152 6 220 440 5 234 2.00e-17 82.0
MS.gene99218.t1 MTR_2g089755 26.585 410 247 20 32 403 37 430 2.24e-17 84.7
MS.gene99218.t1 MTR_1g040195 23.476 443 258 18 33 447 23 412 3.07e-17 84.0
MS.gene99218.t1 MTR_7g015860 22.944 462 282 15 30 447 26 457 5.71e-17 83.2
MS.gene99218.t1 MTR_7g014250 25.598 418 234 18 30 402 24 409 8.57e-17 82.8
MS.gene99218.t1 MTR_8g079980 24.812 399 238 12 33 403 17 381 8.65e-17 82.8
MS.gene99218.t1 MTR_2g089670 25.000 416 242 17 29 402 42 429 1.12e-16 82.4
MS.gene99218.t1 MTR_5g076700 22.247 445 287 16 33 447 24 439 1.72e-16 82.0
MS.gene99218.t1 MTR_3g086020 25.123 406 243 17 62 454 59 416 2.45e-16 81.3
MS.gene99218.t1 MTR_7g114470 23.973 438 269 18 33 437 25 431 3.19e-16 80.9
MS.gene99218.t1 MTR_7g085930 23.786 412 249 14 64 429 59 451 6.34e-16 80.1
MS.gene99218.t1 MTR_4g055170 25.000 304 205 7 64 361 69 355 1.82e-15 78.6
MS.gene99218.t1 MTR_7g086000 25.666 413 254 14 62 442 58 449 2.10e-15 78.6
MS.gene99218.t1 MTR_7g114490 23.714 447 263 18 33 440 24 431 8.86e-15 76.6
MS.gene99218.t1 MTR_4g013050 21.898 411 246 12 57 444 46 404 9.89e-15 76.3
MS.gene99218.t1 MTR_7g014610 23.974 463 276 19 23 434 20 457 1.31e-14 76.3
MS.gene99218.t1 MTR_1g053315 22.051 390 263 15 64 434 78 445 1.69e-14 75.9
MS.gene99218.t1 MTR_7g014230 23.775 408 254 14 30 402 26 411 2.96e-14 75.1
MS.gene99218.t1 MTR_2g089650 24.725 364 240 16 64 403 65 418 3.11e-14 75.1
MS.gene99218.t1 MTR_7g014160 22.833 473 289 18 17 434 4 455 4.47e-14 74.3
MS.gene99218.t1 MTR_3g450710 25.450 389 218 16 58 407 59 414 4.55e-14 74.3
MS.gene99218.t1 MTR_5g090810 25.134 374 225 14 61 403 63 412 6.82e-14 73.9
MS.gene99218.t1 MTR_7g114520 23.153 406 256 17 33 410 25 402 1.61e-13 72.8
MS.gene99218.t1 MTR_3g112180 23.592 284 193 9 61 335 58 326 1.70e-13 72.8
MS.gene99218.t1 MTR_1g040215 20.952 420 262 16 33 421 23 403 3.33e-13 71.6
MS.gene99218.t1 MTR_1g040315 24.092 303 194 10 33 320 23 304 4.82e-13 70.9
MS.gene99218.t1 MTR_7g014200 24.654 434 239 18 23 402 21 420 4.86e-13 71.2
MS.gene99218.t1 MTR_8g467580 26.988 415 232 20 64 449 60 432 1.70e-12 69.3
MS.gene99218.t1 MTR_7g084910 24.373 279 182 8 52 319 58 318 2.15e-12 68.9
MS.gene99218.t1 MTR_7g056087 22.835 381 250 14 64 419 58 419 2.44e-12 68.9
MS.gene99218.t1 MTR_5g090880 23.885 381 230 16 57 403 60 414 4.61e-12 68.2
MS.gene99218.t1 MTR_6g053810 22.819 298 192 9 64 345 71 346 4.69e-12 68.2
MS.gene99218.t1 MTR_3g028210 23.974 463 298 20 4 434 8 448 8.94e-12 67.4
MS.gene99218.t1 MTR_7g026880 57.778 45 19 0 405 449 5 49 1.01e-11 60.1
MS.gene99218.t1 MTR_1g094790 24.824 427 237 21 61 449 57 437 3.62e-11 65.5
MS.gene99218.t1 MTR_7g013850 22.169 415 255 13 37 402 37 432 5.68e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99218.t1 AT5G67150 40.789 456 246 7 2 449 3 442 2.16e-115 346
MS.gene99218.t1 AT3G50300 39.956 458 251 8 1 449 1 443 3.21e-103 315
MS.gene99218.t1 AT3G50280 36.424 453 270 6 1 449 1 439 1.37e-97 300
MS.gene99218.t1 AT3G50270 39.421 449 256 8 6 449 7 444 2.24e-97 300
MS.gene99218.t1 AT5G38130 38.621 435 250 6 5 434 20 442 1.43e-95 296
MS.gene99218.t1 AT5G67160 38.228 429 237 8 25 448 22 427 6.49e-93 288
MS.gene99218.t1 AT5G42830 35.253 434 255 9 28 449 26 445 1.55e-82 261
MS.gene99218.t1 AT2G39980 32.839 472 243 8 30 449 27 476 1.34e-78 252
MS.gene99218.t1 AT5G07860 36.782 435 238 11 32 449 31 445 1.65e-78 251
MS.gene99218.t1 AT5G07850 36.259 433 247 10 32 449 31 449 2.52e-78 251
MS.gene99218.t1 AT5G01210 33.113 453 246 8 36 449 31 465 5.95e-73 238
MS.gene99218.t1 AT5G07870 35.214 443 246 9 32 449 31 457 6.24e-73 237
MS.gene99218.t1 AT3G50280 38.141 312 187 3 141 449 1 309 4.49e-70 225
MS.gene99218.t1 AT5G23940 31.808 437 276 8 28 450 21 449 1.80e-62 210
MS.gene99218.t1 AT3G50290 39.179 268 156 3 138 400 4 269 6.99e-56 187
MS.gene99218.t1 AT2G19070 25.307 407 265 11 59 442 53 443 9.15e-26 109
MS.gene99218.t1 AT3G48720 26.479 355 230 12 57 403 54 385 2.23e-25 108
MS.gene99218.t1 AT3G29590 25.508 443 288 13 24 444 20 442 4.74e-25 107
MS.gene99218.t1 AT5G41040 25.831 391 251 11 57 434 62 426 1.55e-23 102
MS.gene99218.t1 AT5G41040 25.831 391 251 11 57 434 78 442 1.86e-23 102
MS.gene99218.t1 AT5G48930 24.490 392 252 10 64 437 56 421 5.88e-23 101
MS.gene99218.t1 AT1G78990 27.419 372 224 13 51 403 52 396 2.92e-21 96.3
MS.gene99218.t1 AT3G29635 24.138 435 277 15 30 434 27 438 9.82e-21 94.7
MS.gene99218.t1 AT5G57840 27.512 418 237 17 49 434 39 422 1.16e-20 94.4
MS.gene99218.t1 AT5G39080 22.414 464 293 17 20 442 16 453 9.61e-20 92.0
MS.gene99218.t1 AT5G63560 24.297 391 251 14 57 434 54 412 2.32e-19 90.5
MS.gene99218.t1 AT1G24430 23.177 384 244 14 55 426 53 397 1.07e-18 88.6
MS.gene99218.t1 AT3G29670 24.342 456 297 18 3 434 2 433 2.61e-18 87.4
MS.gene99218.t1 AT5G39090 25.114 438 287 19 20 437 16 432 2.74e-18 87.4
MS.gene99218.t1 AT1G31490 25.791 411 257 15 61 454 59 438 3.20e-18 87.0
MS.gene99218.t1 AT5G39050 24.920 313 200 12 151 435 145 450 5.37e-18 86.7
MS.gene99218.t1 AT5G23970 24.312 436 280 17 28 450 20 418 3.73e-17 84.0
MS.gene99218.t1 AT2G40230 24.880 418 268 15 1 403 1 387 1.05e-16 82.4
MS.gene99218.t1 AT3G26040 24.096 415 262 14 52 449 47 425 2.18e-16 81.6
MS.gene99218.t1 AT1G24420 24.936 393 244 16 74 453 75 429 2.28e-16 81.3
MS.gene99218.t1 AT1G24420 24.936 393 244 16 74 453 75 429 2.28e-16 81.3
MS.gene99218.t1 AT3G30280 24.533 428 256 20 1 403 1 386 3.77e-16 80.9
MS.gene99218.t1 AT5G17540 22.799 443 286 17 33 447 23 437 1.43e-15 79.0
MS.gene99218.t1 AT5G47950 22.658 459 301 16 1 449 1 415 3.32e-15 77.8
MS.gene99218.t1 AT1G28680 26.984 252 172 7 157 403 143 387 9.61e-15 76.6
MS.gene99218.t1 AT4G15390 24.090 357 217 16 4 347 8 323 1.53e-14 75.9
MS.gene99218.t1 AT4G15390 24.090 357 217 16 4 347 8 323 1.53e-14 75.9
MS.gene99218.t1 AT5G61160 26.752 314 192 15 151 440 139 438 2.45e-14 75.1
MS.gene99218.t1 AT5G07080 25.484 310 191 12 42 333 44 331 2.74e-14 75.1
MS.gene99218.t1 AT1G28680 27.536 207 143 5 157 359 143 346 2.22e-13 72.0
MS.gene99218.t1 AT3G47170 23.934 305 197 10 57 341 63 352 3.62e-13 71.6
MS.gene99218.t1 AT5G16410 24.545 330 206 13 95 403 112 419 1.22e-12 70.1
MS.gene99218.t1 AT2G25150 25.287 261 181 8 155 406 148 403 2.83e-12 68.9

Find 93 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGTCTTTCTTATGAGATTT+TGG 0.228049 8.4:+26268057 MS.gene99218:CDS
CGTTGAAATGAAGGGAGTTT+TGG 0.257934 8.4:-26267359 None:intergenic
GGAAACGCGGTAGTGGTTAA+TGG 0.258596 8.4:+26267713 MS.gene99218:CDS
TCATTCTTCCCACCCTTTGC+AGG 0.261438 8.4:+26266978 MS.gene99218:CDS
TATGGGAACCTTTCGAGATT+TGG 0.266155 8.4:-26267331 None:intergenic
TACATTCTGCTGAGAAAATA+AGG 0.279160 8.4:+26267813 MS.gene99218:CDS
GGGAAATGATGTTGAGTTCT+TGG 0.297726 8.4:+26268087 MS.gene99218:CDS
TTTGGCATTTCGTTAATTCT+TGG 0.304274 8.4:+26267306 MS.gene99218:CDS
ATGGGAACCTTTCGAGATTT+GGG 0.326542 8.4:-26267330 None:intergenic
TGAGATGGTTGATTTGGTTT+TGG 0.333677 8.4:-26266932 None:intergenic
TAAGGAACCATTACGAATCT+TGG 0.357996 8.4:+26267831 MS.gene99218:CDS
TTTGTTTGAGATGGTTGATT+TGG 0.369144 8.4:-26266938 None:intergenic
ACCAAGGATGATTCCACCTT+TGG 0.373650 8.4:+26267676 MS.gene99218:CDS
ATTGGAATTTCAATATCGTT+TGG 0.377241 8.4:-26267389 None:intergenic
TTGGAATTTCAATATCGTTT+GGG 0.377876 8.4:-26267388 None:intergenic
AAGTACTATTATCTCATGTT+TGG 0.384120 8.4:+26267582 MS.gene99218:CDS
GAAGATTCTTTCAGCTAACT+TGG 0.391044 8.4:-26267474 None:intergenic
TTGTAGACGCCAAGTCGTTT+TGG 0.392431 8.4:+26267288 MS.gene99218:CDS
AGCTTTCTTCTGAGGAGTCT+TGG 0.397249 8.4:-26267445 None:intergenic
TGTTGTGGATATCACCTTGA+TGG 0.409513 8.4:-26266769 None:intergenic
AAAACCAACTCTAGCAATTC+AGG 0.414621 8.4:+26267214 MS.gene99218:CDS
TTAAGTGACGGCATCTTCAT+TGG 0.417429 8.4:+26267245 MS.gene99218:CDS
CCTTTGGAAGAAGATTATTT+CGG 0.422258 8.4:+26267692 MS.gene99218:CDS
GGTTGAAAAGAAGGCCTCTC+TGG 0.429060 8.4:-26266896 None:intergenic
TGTGTGAAAGGGAAGCGTAT+TGG 0.429614 8.4:-26267407 None:intergenic
CAGTCACATTACTTAGAACT+AGG 0.433385 8.4:-26268112 None:intergenic
GTTTGTGGTGTCGTTGTGTT+GGG 0.434146 8.4:-26267015 None:intergenic
CATTCTTCCCACCCTTTGCA+GGG 0.439523 8.4:+26266979 MS.gene99218:CDS
TCCAAAGGTGGAATCATCCT+TGG 0.454320 8.4:-26267677 None:intergenic
GAATGGGTTGGTTGAAAAGA+AGG 0.458480 8.4:-26266905 None:intergenic
TGTTTGTGGTGTCGTTGTGT+TGG 0.462700 8.4:-26267016 None:intergenic
CCGAAATAATCTTCTTCCAA+AGG 0.467292 8.4:-26267692 None:intergenic
AGATTATTATTGTTGTCAAT+TGG 0.472545 8.4:-26266804 None:intergenic
GGGAACCAACGTTGAAATGA+AGG 0.474068 8.4:-26267368 None:intergenic
GACAATTGGAGGTGTGTAGT+TGG 0.479530 8.4:-26267144 None:intergenic
TGGAAAGTGTTTGTTTGAGA+TGG 0.485281 8.4:-26266947 None:intergenic
TTCTCAACCAAGATTCGTAA+TGG 0.489014 8.4:-26267838 None:intergenic
GAGGTTCTAGAGTGTGAAAT+CGG 0.491146 8.4:+26267755 MS.gene99218:CDS
AACGAAATGCCAAAACGACT+TGG 0.492743 8.4:-26267297 None:intergenic
TGTGATTGTATTTGTGTGAA+AGG 0.505410 8.4:-26267419 None:intergenic
TTATTTCGGAAACGCGGTAG+TGG 0.506417 8.4:+26267706 MS.gene99218:CDS
CTTCTTCATGGATTCCATCA+AGG 0.511613 8.4:+26266755 None:intergenic
AATGGTGTGAGGATGAAAGC+AGG 0.512088 8.4:+26267731 MS.gene99218:CDS
GTTACCTGAATTGCTAGAGT+TGG 0.515092 8.4:-26267218 None:intergenic
TGGTTTCAAGTGGAAGGAAT+CGG 0.519621 8.4:-26266872 None:intergenic
GAGGTGTGTAGTTGGATTGA+AGG 0.522441 8.4:-26267136 None:intergenic
GGATTCTAAGCTTTCTTCTG+AGG 0.527823 8.4:-26267453 None:intergenic
GTGATTGTATTTGTGTGAAA+GGG 0.534325 8.4:-26267418 None:intergenic
CTCCCACTTCGAAACGCCAC+AGG 0.534418 8.4:-26267965 None:intergenic
TTCCGAAGTGGTAGTTCGCT+AGG 0.540203 8.4:+26267872 MS.gene99218:CDS
ACCGAGGTTGTTGCAAATGA+TGG 0.541381 8.4:-26267066 None:intergenic
GATTACAAGACGCCCTGCAA+AGG 0.543778 8.4:-26266991 None:intergenic
AGCCTGTGGCGTTTCGAAGT+GGG 0.545492 8.4:+26267963 MS.gene99218:CDS
AACTCCCTTCATTTCAACGT+TGG 0.545872 8.4:+26267363 MS.gene99218:CDS
GTGGCGTTTCGAAGTGGGAG+TGG 0.549176 8.4:+26267968 MS.gene99218:CDS
ATGGTGTGAGGATGAAAGCA+GGG 0.551554 8.4:+26267732 MS.gene99218:CDS
GTGTTTGCTGGTGCTGAAGA+AGG 0.553326 8.4:+26268016 MS.gene99218:CDS
ATTACAAGACGCCCTGCAAA+GGG 0.555357 8.4:-26266990 None:intergenic
GAAAAGAAGGCCTCTCTGGA+TGG 0.557292 8.4:-26266892 None:intergenic
TTCGCTAGGTGGTAATATGT+TGG 0.564328 8.4:+26267886 MS.gene99218:CDS
AAATAATCTTCTTCCAAAGG+TGG 0.566011 8.4:-26267689 None:intergenic
TGGTGCTTGAATTGTTGTTG+TGG 0.571268 8.4:-26266784 None:intergenic
TCTGGATGGTTTCAAGTGGA+AGG 0.572079 8.4:-26266878 None:intergenic
GGAACCAACGTTGAAATGAA+GGG 0.576282 8.4:-26267367 None:intergenic
TCTTGGTCCCAAATCTCGAA+AGG 0.578370 8.4:+26267323 MS.gene99218:CDS
AACTCTAGCAATTCAGGTAA+CGG 0.581378 8.4:+26267220 MS.gene99218:CDS
GAAAATTGATCTAACACCAT+GGG 0.583475 8.4:+26266845 MS.gene99218:CDS
CACCTAGCGAACTACCACTT+CGG 0.583535 8.4:-26267874 None:intergenic
TCCATCATTTGCAACAACCT+CGG 0.586818 8.4:+26267065 MS.gene99218:CDS
GGGTGGGAAGAATGAGAGTG+TGG 0.597075 8.4:-26266970 None:intergenic
GGAAGGAATCGGAGATCCCA+TGG 0.597088 8.4:-26266861 None:intergenic
AAGCCTGTGGCGTTTCGAAG+TGG 0.599152 8.4:+26267962 MS.gene99218:CDS
ACATAGAAAACATCATCTTG+TGG 0.599444 8.4:-26267635 None:intergenic
ACAAGACGCCCTGCAAAGGG+TGG 0.600540 8.4:-26266987 None:intergenic
CCTCTCTGGATGGTTTCAAG+TGG 0.606841 8.4:-26266882 None:intergenic
ACACCAACGTTGTTCCGAAG+TGG 0.610004 8.4:+26267860 MS.gene99218:CDS
TGGGAAGAATGAGAGTGTGG+TGG 0.610635 8.4:-26266967 None:intergenic
AGAAAATTGATCTAACACCA+TGG 0.626932 8.4:+26266844 MS.gene99218:CDS
CTACCACTTCGGAACAACGT+TGG 0.629293 8.4:-26267863 None:intergenic
CAAGACGCCCTGCAAAGGGT+GGG 0.638377 8.4:-26266986 None:intergenic
AAGAATCTTCCATTTCACAA+AGG 0.647468 8.4:+26267487 MS.gene99218:CDS
TGGTGTGAGGATGAAAGCAG+GGG 0.647651 8.4:+26267733 MS.gene99218:CDS
CGAAGTGGTAGTTCGCTAGG+TGG 0.653912 8.4:+26267875 MS.gene99218:CDS
CAGGTAACGGAGTTAAGTGA+CGG 0.655221 8.4:+26267233 MS.gene99218:CDS
AGCACAAAACACAACTGTCG+CGG 0.659280 8.4:+26267109 MS.gene99218:CDS
TGTGAGGATGAAAGCAGGGG+AGG 0.662636 8.4:+26267736 MS.gene99218:CDS
CGGTAGTGGTTAATGGTGTG+AGG 0.683331 8.4:+26267720 MS.gene99218:CDS
TTTCACAAAGGAGAAAATCG+CGG 0.713213 8.4:+26267499 MS.gene99218:CDS
AGAAGATTATTTCGGAAACG+CGG 0.717549 8.4:+26267700 MS.gene99218:CDS
CCACTTGAAACCATCCAGAG+AGG 0.728529 8.4:+26266882 MS.gene99218:CDS
ACTTGGCGTCTACAACAGCG+TGG 0.778771 8.4:-26267280 None:intergenic
TGATGATAGATGTTAGACCA+AGG 0.780037 8.4:+26267660 MS.gene99218:CDS
CGTGGACGAAGAGTGCACCG+AGG 0.794757 8.4:-26267082 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTAGATTTATCTGTTT+CGG - chr8.4:26267552-26267571 None:intergenic 15.0%
!! AGATTATTATTGTTGTCAAT+TGG - chr8.4:26266807-26266826 None:intergenic 20.0%
! AAGTACTATTATCTCATGTT+TGG + chr8.4:26267582-26267601 MS.gene99218:CDS 25.0%
! ATTGGAATTTCAATATCGTT+TGG - chr8.4:26267392-26267411 None:intergenic 25.0%
! TTGGAATTTCAATATCGTTT+GGG - chr8.4:26267391-26267410 None:intergenic 25.0%
!!! CACTCATTTTTTACACTTAA+CGG + chr8.4:26267167-26267186 MS.gene99218:CDS 25.0%
AAATAATCTTCTTCCAAAGG+TGG - chr8.4:26267692-26267711 None:intergenic 30.0%
AAGAATCTTCCATTTCACAA+AGG + chr8.4:26267487-26267506 MS.gene99218:CDS 30.0%
ACATAGAAAACATCATCTTG+TGG - chr8.4:26267638-26267657 None:intergenic 30.0%
AGAAAATTGATCTAACACCA+TGG + chr8.4:26266844-26266863 MS.gene99218:CDS 30.0%
CGTTAAGTGTAAAAAATGAG+TGG - chr8.4:26267169-26267188 None:intergenic 30.0%
GAAAATTGATCTAACACCAT+GGG + chr8.4:26266845-26266864 MS.gene99218:CDS 30.0%
GGGAGTGGTAATAAAAATAA+TGG + chr8.4:26267983-26268002 MS.gene99218:CDS 30.0%
GTAAAAAATGAGTGGACAAT+TGG - chr8.4:26267161-26267180 None:intergenic 30.0%
GTGATTGTATTTGTGTGAAA+GGG - chr8.4:26267421-26267440 None:intergenic 30.0%
TACATTCTGCTGAGAAAATA+AGG + chr8.4:26267813-26267832 MS.gene99218:CDS 30.0%
TGTGATTGTATTTGTGTGAA+AGG - chr8.4:26267422-26267441 None:intergenic 30.0%
TTGCCAAAAACATCAAATCT+CGG - chr8.4:26267926-26267945 None:intergenic 30.0%
TTTGGCATTTCGTTAATTCT+TGG + chr8.4:26267306-26267325 MS.gene99218:CDS 30.0%
! CCTTTGGAAGAAGATTATTT+CGG + chr8.4:26267692-26267711 MS.gene99218:CDS 30.0%
! GTGTCTTTCTTATGAGATTT+TGG + chr8.4:26268057-26268076 MS.gene99218:CDS 30.0%
! TCTTTCTTATGAGATTTTGG+AGG + chr8.4:26268060-26268079 MS.gene99218:CDS 30.0%
! TTTGTTTGAGATGGTTGATT+TGG - chr8.4:26266941-26266960 None:intergenic 30.0%
!! AAGGGAGTTTTGGTATTTTA+TGG - chr8.4:26267352-26267371 None:intergenic 30.0%
!! AGGGAGTTTTGGTATTTTAT+GGG - chr8.4:26267351-26267370 None:intergenic 30.0%
!! TTTTTGGCAATGATTTTGGT+TGG + chr8.4:26267936-26267955 MS.gene99218:CDS 30.0%
!!! ATTTGGTTTTGGTTAGGAAT+GGG - chr8.4:26266924-26266943 None:intergenic 30.0%
!!! GATGTTTTTGGCAATGATTT+TGG + chr8.4:26267932-26267951 MS.gene99218:CDS 30.0%
!!! TTTTGGCAATGATTTTGGTT+GGG + chr8.4:26267937-26267956 MS.gene99218:CDS 30.0%
AAAAATGAGTGGACAATTGG+AGG - chr8.4:26267158-26267177 None:intergenic 35.0%
AAAACCAACTCTAGCAATTC+AGG + chr8.4:26267214-26267233 MS.gene99218:CDS 35.0%
AACTCTAGCAATTCAGGTAA+CGG + chr8.4:26267220-26267239 MS.gene99218:CDS 35.0%
AGAAGATTATTTCGGAAACG+CGG + chr8.4:26267700-26267719 MS.gene99218:CDS 35.0%
CCGAAATAATCTTCTTCCAA+AGG - chr8.4:26267695-26267714 None:intergenic 35.0%
GAAGATTCTTTCAGCTAACT+TGG - chr8.4:26267477-26267496 None:intergenic 35.0%
GGAAAAATTAGTGTGTTTGC+TGG + chr8.4:26268004-26268023 MS.gene99218:CDS 35.0%
TAAGGAACCATTACGAATCT+TGG + chr8.4:26267831-26267850 MS.gene99218:CDS 35.0%
TGATGATAGATGTTAGACCA+AGG + chr8.4:26267660-26267679 MS.gene99218:CDS 35.0%
TTCTCAACCAAGATTCGTAA+TGG - chr8.4:26267841-26267860 None:intergenic 35.0%
TTTCACAAAGGAGAAAATCG+CGG + chr8.4:26267499-26267518 MS.gene99218:CDS 35.0%
! CGATTTTCTCCTTTGTGAAA+TGG - chr8.4:26267499-26267518 None:intergenic 35.0%
! TCTCCGAGATTTGATGTTTT+TGG + chr8.4:26267920-26267939 MS.gene99218:CDS 35.0%
!! GTTGTTGTTGTTGTTGTTTG+TGG - chr8.4:26267033-26267052 None:intergenic 35.0%
!! TATGAGATTTTGGAGGCTTT+GGG + chr8.4:26268067-26268086 MS.gene99218:CDS 35.0%
!! TGGAAAGTGTTTGTTTGAGA+TGG - chr8.4:26266950-26266969 None:intergenic 35.0%
!! TTATGAGATTTTGGAGGCTT+TGG + chr8.4:26268066-26268085 MS.gene99218:CDS 35.0%
!!! GATTTGGTTTTGGTTAGGAA+TGG - chr8.4:26266925-26266944 None:intergenic 35.0%
!!! TGAGATGGTTGATTTGGTTT+TGG - chr8.4:26266935-26266954 None:intergenic 35.0%
!!! TGGTTGATTTGGTTTTGGTT+AGG - chr8.4:26266930-26266949 None:intergenic 35.0%
!!! TTTGGCAATGATTTTGGTTG+GGG + chr8.4:26267938-26267957 MS.gene99218:CDS 35.0%
AACGAAATGCCAAAACGACT+TGG - chr8.4:26267300-26267319 None:intergenic 40.0%
AACTCCCTTCATTTCAACGT+TGG + chr8.4:26267363-26267382 MS.gene99218:CDS 40.0%
ATGGGAACCTTTCGAGATTT+GGG - chr8.4:26267333-26267352 None:intergenic 40.0%
GGAACCAACGTTGAAATGAA+GGG - chr8.4:26267370-26267389 None:intergenic 40.0%
GGATTCTAAGCTTTCTTCTG+AGG - chr8.4:26267456-26267475 None:intergenic 40.0%
GGGAAATGATGTTGAGTTCT+TGG + chr8.4:26268087-26268106 MS.gene99218:CDS 40.0%
GTTACCTGAATTGCTAGAGT+TGG - chr8.4:26267221-26267240 None:intergenic 40.0%
TATGGGAACCTTTCGAGATT+TGG - chr8.4:26267334-26267353 None:intergenic 40.0%
TCCATCATTTGCAACAACCT+CGG + chr8.4:26267065-26267084 MS.gene99218:CDS 40.0%
TGTTGTGGATATCACCTTGA+TGG - chr8.4:26266772-26266791 None:intergenic 40.0%
TTAAGTGACGGCATCTTCAT+TGG + chr8.4:26267245-26267264 MS.gene99218:CDS 40.0%
TTCGCTAGGTGGTAATATGT+TGG + chr8.4:26267886-26267905 MS.gene99218:CDS 40.0%
! CGTTGAAATGAAGGGAGTTT+TGG - chr8.4:26267362-26267381 None:intergenic 40.0%
! TGGTGCTTGAATTGTTGTTG+TGG - chr8.4:26266787-26266806 None:intergenic 40.0%
! TGGTTTCAAGTGGAAGGAAT+CGG - chr8.4:26266875-26266894 None:intergenic 40.0%
!! GAATGGGTTGGTTGAAAAGA+AGG - chr8.4:26266908-26266927 None:intergenic 40.0%
!! GAGGTTCTAGAGTGTGAAAT+CGG + chr8.4:26267755-26267774 MS.gene99218:CDS 40.0%
AATGGTGTGAGGATGAAAGC+AGG + chr8.4:26267731-26267750 MS.gene99218:CDS 45.0%
ACCAAGGATGATTCCACCTT+TGG + chr8.4:26267676-26267695 MS.gene99218:CDS 45.0%
ACCGAGGTTGTTGCAAATGA+TGG - chr8.4:26267069-26267088 None:intergenic 45.0%
AGCACAAAACACAACTGTCG+CGG + chr8.4:26267109-26267128 MS.gene99218:CDS 45.0%
ATGGTGTGAGGATGAAAGCA+GGG + chr8.4:26267732-26267751 MS.gene99218:CDS 45.0%
ATTACAAGACGCCCTGCAAA+GGG - chr8.4:26266993-26267012 None:intergenic 45.0%
GAGGTGTGTAGTTGGATTGA+AGG - chr8.4:26267139-26267158 None:intergenic 45.0%
GGGAACCAACGTTGAAATGA+AGG - chr8.4:26267371-26267390 None:intergenic 45.0%
TCCAAAGGTGGAATCATCCT+TGG - chr8.4:26267680-26267699 None:intergenic 45.0%
TCTTGGTCCCAAATCTCGAA+AGG + chr8.4:26267323-26267342 MS.gene99218:CDS 45.0%
TTATTTCGGAAACGCGGTAG+TGG + chr8.4:26267706-26267725 MS.gene99218:CDS 45.0%
! AGCTTTCTTCTGAGGAGTCT+TGG - chr8.4:26267448-26267467 None:intergenic 45.0%
! GACAATTGGAGGTGTGTAGT+TGG - chr8.4:26267147-26267166 None:intergenic 45.0%
! GACAGTTGTGTTTTGTGCTG+TGG - chr8.4:26267108-26267127 None:intergenic 45.0%
! TCTGGATGGTTTCAAGTGGA+AGG - chr8.4:26266881-26266900 None:intergenic 45.0%
! TGTGTGAAAGGGAAGCGTAT+TGG - chr8.4:26267410-26267429 None:intergenic 45.0%
! TTGTAGACGCCAAGTCGTTT+TGG + chr8.4:26267288-26267307 MS.gene99218:CDS 45.0%
!! CAGGTAACGGAGTTAAGTGA+CGG + chr8.4:26267233-26267252 MS.gene99218:CDS 45.0%
!! GTTTGTGGTGTCGTTGTGTT+GGG - chr8.4:26267018-26267037 None:intergenic 45.0%
!! TGTTTGTGGTGTCGTTGTGT+TGG - chr8.4:26267019-26267038 None:intergenic 45.0%
!!! GGTTTTGGTTAGGAATGGGT+TGG - chr8.4:26266920-26266939 None:intergenic 45.0%
ACACCAACGTTGTTCCGAAG+TGG + chr8.4:26267860-26267879 MS.gene99218:CDS 50.0%
CACCTAGCGAACTACCACTT+CGG - chr8.4:26267877-26267896 None:intergenic 50.0%
CATTCTTCCCACCCTTTGCA+GGG + chr8.4:26266979-26266998 MS.gene99218:CDS 50.0%
CCACTTGAAACCATCCAGAG+AGG + chr8.4:26266882-26266901 MS.gene99218:CDS 50.0%
CCTCTCTGGATGGTTTCAAG+TGG - chr8.4:26266885-26266904 None:intergenic 50.0%
CTACCACTTCGGAACAACGT+TGG - chr8.4:26267866-26267885 None:intergenic 50.0%
GAAAAGAAGGCCTCTCTGGA+TGG - chr8.4:26266895-26266914 None:intergenic 50.0%
GATTACAAGACGCCCTGCAA+AGG - chr8.4:26266994-26267013 None:intergenic 50.0%
GGTTGAAAAGAAGGCCTCTC+TGG - chr8.4:26266899-26266918 None:intergenic 50.0%
TCATTCTTCCCACCCTTTGC+AGG + chr8.4:26266978-26266997 MS.gene99218:CDS 50.0%
TGGTGTGAGGATGAAAGCAG+GGG + chr8.4:26267733-26267752 MS.gene99218:CDS 50.0%
TTCCGAAGTGGTAGTTCGCT+AGG + chr8.4:26267872-26267891 MS.gene99218:CDS 50.0%
! CGGTAGTGGTTAATGGTGTG+AGG + chr8.4:26267720-26267739 MS.gene99218:CDS 50.0%
! GGAAACGCGGTAGTGGTTAA+TGG + chr8.4:26267713-26267732 MS.gene99218:CDS 50.0%
! GTGTTTGCTGGTGCTGAAGA+AGG + chr8.4:26268016-26268035 MS.gene99218:CDS 50.0%
! TGGGAAGAATGAGAGTGTGG+TGG - chr8.4:26266970-26266989 None:intergenic 50.0%
! TTGTGTTTTGTGCTGTGGCG+TGG - chr8.4:26267103-26267122 None:intergenic 50.0%
!! TTTTGGTTGGGGAAAGCCTG+TGG + chr8.4:26267949-26267968 MS.gene99218:CDS 50.0%
AAGCCTGTGGCGTTTCGAAG+TGG + chr8.4:26267962-26267981 MS.gene99218:CDS 55.0%
ACTTGGCGTCTACAACAGCG+TGG - chr8.4:26267283-26267302 None:intergenic 55.0%
AGCCTGTGGCGTTTCGAAGT+GGG + chr8.4:26267963-26267982 MS.gene99218:CDS 55.0%
CGAAGTGGTAGTTCGCTAGG+TGG + chr8.4:26267875-26267894 MS.gene99218:CDS 55.0%
GGAAGGAATCGGAGATCCCA+TGG - chr8.4:26266864-26266883 None:intergenic 55.0%
GGGTGGGAAGAATGAGAGTG+TGG - chr8.4:26266973-26266992 None:intergenic 55.0%
TGTGAGGATGAAAGCAGGGG+AGG + chr8.4:26267736-26267755 MS.gene99218:CDS 55.0%
ACAAGACGCCCTGCAAAGGG+TGG - chr8.4:26266990-26267009 None:intergenic 60.0%
CAAGACGCCCTGCAAAGGGT+GGG - chr8.4:26266989-26267008 None:intergenic 60.0%
CTCCCACTTCGAAACGCCAC+AGG - chr8.4:26267968-26267987 None:intergenic 60.0%
GTGGCGTTTCGAAGTGGGAG+TGG + chr8.4:26267968-26267987 MS.gene99218:CDS 60.0%
! CGTGGACGAAGAGTGCACCG+AGG - chr8.4:26267085-26267104 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 26266762 26268126 26266762 ID=MS.gene99218
chr8.4 mRNA 26266762 26268126 26266762 ID=MS.gene99218.t1;Parent=MS.gene99218
chr8.4 exon 26266762 26268126 26266762 ID=MS.gene99218.t1.exon1;Parent=MS.gene99218.t1
chr8.4 CDS 26266762 26268126 26266762 ID=cds.MS.gene99218.t1;Parent=MS.gene99218.t1
Gene Sequence

>MS.gene99218

ATGGATTCCATCAAGGTGATATCCACAACAACAATTCAAGCACCAATTGACAACAATAATAATCTCAATTCAACTGATTATCAGAAAATTGATCTAACACCATGGGATCTCCGATTCCTTCCACTTGAAACCATCCAGAGAGGCCTTCTTTTCAACCAACCCATTCCTAACCAAAACCAAATCAACCATCTCAAACAAACACTTTCCACCACACTCTCATTCTTCCCACCCTTTGCAGGGCGTCTTGTAATCACCCAACACAACGACACCACAAACAACAACAACAACAACAACACATCATGTTCCATCATTTGCAACAACCTCGGTGCACTCTTCGTCCACGCCACAGCACAAAACACAACTGTCGCGGACATCCTTCAATCCAACTACACACCTCCAATTGTCCACTCATTTTTTACACTTAACGGTGTTAAAAACTACGAAGCTACGTCAAAACCAACTCTAGCAATTCAGGTAACGGAGTTAAGTGACGGCATCTTCATTGGCTTCACATGCAACCACGCTGTTGTAGACGCCAAGTCGTTTTGGCATTTCGTTAATTCTTGGTCCCAAATCTCGAAAGGTTCCCATAAAATACCAAAACTCCCTTCATTTCAACGTTGGTTCCCAAACGATATTGAAATTCCAATACGCTTCCCTTTCACACAAATACAATCACAAAACCAAGACTCCTCAGAAGAAAGCTTAGAATCCAAGTTAGCTGAAAGAATCTTCCATTTCACAAAGGAGAAAATCGCGGAACTGAAATCAAAAGCAAACGCAGAAGCCGAAACAGATAAATCTAAAATATCTTCGCTTCAAGTACTATTATCTCATGTTTGGCGTTCTGTTGTTCGTTGCAAAAAACACGATCCACAAGATGATGTTTTCTATGTGTTGATGATAGATGTTAGACCAAGGATGATTCCACCTTTGGAAGAAGATTATTTCGGAAACGCGGTAGTGGTTAATGGTGTGAGGATGAAAGCAGGGGAGGTTCTAGAGTGTGAAATCGGAAAAGTTGCTTTAGAGATAAACAAGATGATTTCGTTACATTCTGCTGAGAAAATAAGGAACCATTACGAATCTTGGTTGAGAACACCAACGTTGTTCCGAAGTGGTAGTTCGCTAGGTGGTAATATGTTGGCAATGAGCAGTTCTCCGAGATTTGATGTTTTTGGCAATGATTTTGGTTGGGGAAAGCCTGTGGCGTTTCGAAGTGGGAGTGGTAATAAAAATAATGGAAAAATTAGTGTGTTTGCTGGTGCTGAAGAAGGTAGTATAGATGTTGAAATGTGTCTTTCTTATGAGATTTTGGAGGCTTTGGGAAATGATGTTGAGTTCTTGGTTCCTAGTTCTAAGTAA
Protein sequence

>MS.gene99218.t1

MDSIKVISTTTIQAPIDNNNNLNSTDYQKIDLTPWDLRFLPLETIQRGLLFNQPIPNQNQINHLKQTLSTTLSFFPPFAGRLVITQHNDTTNNNNNNNTSCSIICNNLGALFVHATAQNTTVADILQSNYTPPIVHSFFTLNGVKNYEATSKPTLAIQVTELSDGIFIGFTCNHAVVDAKSFWHFVNSWSQISKGSHKIPKLPSFQRWFPNDIEIPIRFPFTQIQSQNQDSSEESLESKLAERIFHFTKEKIAELKSKANAEAETDKSKISSLQVLLSHVWRSVVRCKKHDPQDDVFYVLMIDVRPRMIPPLEEDYFGNAVVVNGVRMKAGEVLECEIGKVALEINKMISLHSAEKIRNHYESWLRTPTLFRSGSSLGGNMLAMSSSPRFDVFGNDFGWGKPVAFRSGSGNKNNGKISVFAGAEEGSIDVEMCLSYEILEALGNDVEFLVPSSK