AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032349

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032349.t1 MTR_0190s0020 93.948 347 21 0 1 347 97 443 0.0 682
MS.gene032349.t1 MTR_8g041770 71.304 345 98 1 2 346 98 441 2.68e-179 504
MS.gene032349.t1 MTR_4g022800 60.784 357 127 4 1 346 88 442 4.54e-156 445
MS.gene032349.t1 MTR_4g094832 61.960 347 123 4 1 342 84 426 4.72e-156 445
MS.gene032349.t1 MTR_4g022670 60.393 356 129 4 1 346 88 441 5.83e-154 440
MS.gene032349.t1 MTR_3g088420 61.671 347 129 3 2 346 89 433 4.86e-153 437
MS.gene032349.t1 MTR_4g022720 60.571 350 132 4 1 346 88 435 5.04e-152 435
MS.gene032349.t1 MTR_7g026860 59.664 357 127 5 1 346 88 438 9.72e-152 434
MS.gene032349.t1 MTR_4g022825 58.989 356 131 5 2 346 89 440 1.43e-151 434
MS.gene032349.t1 MTR_3g026310 58.564 362 131 4 2 346 89 448 1.97e-150 431
MS.gene032349.t1 MTR_4g094835 60.231 347 129 4 1 342 100 442 4.23e-150 430
MS.gene032349.t1 MTR_4g094842 56.522 345 149 1 1 344 102 446 7.71e-142 409
MS.gene032349.t1 MTR_4g094838 55.620 347 129 4 1 342 102 428 2.73e-134 389
MS.gene032349.t1 MTR_4g094828 50.278 360 163 7 1 346 93 450 1.29e-122 360
MS.gene032349.t1 MTR_8g079710 51.724 348 153 7 1 337 93 436 4.22e-121 356
MS.gene032349.t1 MTR_4g022760 51.549 355 118 4 1 346 88 397 5.06e-121 354
MS.gene032349.t1 MTR_4g022290 50.281 356 157 7 1 343 94 442 2.98e-116 344
MS.gene032349.t1 MTR_6g016815 45.115 348 119 7 2 346 103 381 6.85e-91 277
MS.gene032349.t1 MTR_8g040780 58.986 217 86 2 128 341 11 227 7.33e-89 267
MS.gene032349.t1 MTR_3g101900 42.120 349 193 6 3 346 104 448 1.20e-85 266
MS.gene032349.t1 MTR_3g101930 41.880 351 192 6 3 345 108 454 1.38e-84 263
MS.gene032349.t1 MTR_3g102010 40.960 354 195 6 3 346 102 451 6.51e-79 249
MS.gene032349.t1 MTR_3g102020 40.286 350 199 6 3 346 106 451 4.45e-78 246
MS.gene032349.t1 MTR_3g101960 39.710 345 198 6 3 341 105 445 3.72e-76 241
MS.gene032349.t1 MTR_3g101970 39.420 345 193 6 3 345 106 436 1.13e-75 240
MS.gene032349.t1 MTR_3g070170 31.443 388 210 5 2 346 96 470 9.26e-63 207
MS.gene032349.t1 MTR_4g015180 34.729 406 196 10 2 346 90 487 2.08e-62 207
MS.gene032349.t1 MTR_5g081960 32.658 395 209 11 2 342 91 482 4.03e-56 190
MS.gene032349.t1 MTR_7g026770 50.262 191 72 3 54 242 132 301 2.60e-55 183
MS.gene032349.t1 MTR_4g094825 52.980 151 59 2 197 346 252 391 1.91e-47 165
MS.gene032349.t1 MTR_4g094825 52.991 117 52 1 1 114 160 276 3.20e-34 130
MS.gene032349.t1 MTR_3g102030 33.993 303 165 7 3 300 48 320 2.07e-47 163
MS.gene032349.t1 MTR_6g034485 54.762 126 56 1 99 224 1 125 2.47e-47 156
MS.gene032349.t1 MTR_6g045553 30.659 349 202 6 31 342 126 471 4.03e-47 166
MS.gene032349.t1 MTR_6g034495 77.320 97 20 1 2 96 71 167 9.73e-47 156
MS.gene032349.t1 MTR_8g040760 64.407 118 41 1 2 118 95 212 1.65e-43 150
MS.gene032349.t1 MTR_7g026850 68.817 93 29 0 1 93 71 163 4.13e-41 142
MS.gene032349.t1 MTR_8g075610 27.143 350 222 13 2 333 77 411 2.30e-29 117
MS.gene032349.t1 MTR_2g064495 26.571 350 227 11 2 333 86 423 3.38e-29 117
MS.gene032349.t1 MTR_2g105330 26.571 350 227 11 2 333 86 423 6.40e-29 116
MS.gene032349.t1 MTR_7g026830 64.103 78 28 0 1 78 20 97 1.28e-28 107
MS.gene032349.t1 MTR_4g007540 25.284 352 230 12 2 333 86 424 1.90e-28 114
MS.gene032349.t1 MTR_3g098980 29.532 342 214 13 5 333 99 426 1.98e-28 114
MS.gene032349.t1 MTR_3g026950 29.688 320 176 12 50 331 129 437 2.77e-28 114
MS.gene032349.t1 MTR_3g049150 29.245 318 181 10 50 333 130 437 1.70e-26 109
MS.gene032349.t1 MTR_7g026780 48.214 112 33 2 235 346 25 111 1.10e-24 97.1
MS.gene032349.t1 MTR_8g096530 27.907 344 224 11 2 333 89 420 1.64e-24 103
MS.gene032349.t1 MTR_7g084940 29.126 309 188 11 2 295 100 392 2.38e-21 94.7
MS.gene032349.t1 MTR_7g026870 68.519 54 17 0 202 255 98 151 5.46e-21 88.6
MS.gene032349.t1 MTR_4g097090 27.303 304 191 12 50 327 141 440 1.31e-20 92.8
MS.gene032349.t1 MTR_7g027175 26.513 347 225 10 2 333 98 429 3.35e-20 91.7
MS.gene032349.t1 MTR_4g127650 24.501 351 234 12 2 327 96 440 1.50e-19 89.7
MS.gene032349.t1 MTR_1g040195 27.978 361 205 17 2 333 71 405 4.45e-19 88.2
MS.gene032349.t1 MTR_4g013050 24.789 355 215 10 2 337 83 404 5.17e-19 87.8
MS.gene032349.t1 MTR_7g014250 29.021 286 154 12 51 299 140 413 1.66e-18 86.7
MS.gene032349.t1 MTR_3g049140 29.167 240 131 10 116 331 6 230 2.57e-18 83.6
MS.gene032349.t1 MTR_6g015315 26.498 317 191 10 51 336 140 445 2.96e-18 85.9
MS.gene032349.t1 MTR_6g015320 27.742 310 189 11 51 333 143 444 1.27e-17 84.0
MS.gene032349.t1 MTR_1g040175 26.197 355 225 17 2 335 94 432 1.49e-17 84.0
MS.gene032349.t1 MTR_2g015430 27.715 267 175 7 42 298 128 386 5.71e-17 82.0
MS.gene032349.t1 MTR_7g086000 25.824 364 218 14 2 327 92 441 1.28e-16 80.9
MS.gene032349.t1 MTR_2g089755 27.698 278 167 10 50 299 162 433 1.81e-16 80.5
MS.gene032349.t1 MTR_3g087640 28.221 326 195 14 38 333 146 462 2.99e-16 80.1
MS.gene032349.t1 MTR_7g014230 29.102 323 166 16 43 319 134 439 8.19e-16 78.6
MS.gene032349.t1 MTR_6g015850 26.299 308 197 10 51 334 147 448 2.35e-15 77.0
MS.gene032349.t1 MTR_2g089765 25.625 320 188 9 50 330 159 467 1.06e-14 75.5
MS.gene032349.t1 MTR_7g014360 29.104 268 157 11 51 299 90 343 2.39e-14 73.9
MS.gene032349.t1 MTR_1g081660 25.309 324 197 14 2 305 104 402 3.29e-14 73.6
MS.gene032349.t1 MTR_7g015860 25.347 288 164 10 45 296 141 413 3.34e-14 73.6
MS.gene032349.t1 MTR_7g026880 59.259 54 20 1 293 346 2 53 4.07e-14 66.6
MS.gene032349.t1 MTR_1g040215 25.000 320 189 12 2 296 94 387 8.31e-14 72.4
MS.gene032349.t1 MTR_4g055170 28.378 222 142 5 45 255 140 355 1.09e-13 72.0
MS.gene032349.t1 MTR_3g086020 24.857 350 237 10 2 346 87 415 2.07e-13 71.2
MS.gene032349.t1 MTR_3g465980 26.943 193 130 3 45 228 141 331 3.91e-13 70.5
MS.gene032349.t1 MTR_7g085930 26.667 270 173 8 2 249 89 355 1.10e-12 68.9
MS.gene032349.t1 MTR_7g056087 23.457 243 168 6 2 228 90 330 1.73e-12 68.6
MS.gene032349.t1 MTR_3g465990 27.273 198 131 5 41 228 137 331 3.28e-12 67.8
MS.gene032349.t1 MTR_3g112180 25.478 314 192 11 2 294 96 388 4.02e-12 67.4
MS.gene032349.t1 MTR_5g026320 24.865 370 234 14 1 346 104 453 5.82e-12 67.0
MS.gene032349.t1 MTR_2g089670 23.551 276 169 7 50 295 166 429 2.34e-11 65.1
MS.gene032349.t1 MTR_7g084910 27.803 223 144 7 2 212 101 318 2.38e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032349.t1 AT5G67150 40.571 350 196 6 2 342 96 442 7.79e-85 264
MS.gene032349.t1 AT3G50280 38.109 349 207 6 2 343 94 440 1.30e-80 253
MS.gene032349.t1 AT3G50300 40.336 357 196 9 2 345 94 446 3.72e-80 251
MS.gene032349.t1 AT5G67160 40.286 350 197 7 2 346 90 432 8.62e-78 245
MS.gene032349.t1 AT5G07860 40.227 353 191 7 2 345 107 448 2.81e-75 239
MS.gene032349.t1 AT3G50270 37.110 353 213 6 2 346 97 448 5.83e-75 238
MS.gene032349.t1 AT5G01210 36.198 384 202 6 1 346 91 469 4.43e-74 237
MS.gene032349.t1 AT5G42830 39.496 357 198 8 2 346 99 449 2.76e-73 234
MS.gene032349.t1 AT3G50280 38.462 312 184 5 38 343 1 310 6.24e-72 226
MS.gene032349.t1 AT5G07850 40.056 357 194 9 2 346 105 453 3.11e-69 224
MS.gene032349.t1 AT5G07870 37.741 363 200 7 2 346 107 461 6.99e-68 220
MS.gene032349.t1 AT2G39980 34.596 396 201 8 2 346 92 480 1.53e-65 215
MS.gene032349.t1 AT5G38130 37.982 337 190 9 2 327 114 442 7.57e-64 210
MS.gene032349.t1 AT5G23940 35.443 316 189 6 39 342 136 448 9.75e-58 194
MS.gene032349.t1 AT3G50290 41.948 267 146 5 35 293 4 269 2.45e-55 182
MS.gene032349.t1 AT5G48930 26.705 352 225 11 2 333 86 424 4.58e-27 111
MS.gene032349.t1 AT3G48720 25.513 341 238 8 2 334 91 423 1.72e-24 103
MS.gene032349.t1 AT2G19070 27.322 366 222 14 2 334 88 442 6.93e-23 99.4
MS.gene032349.t1 AT2G40230 28.846 312 198 11 2 300 91 391 7.30e-22 96.3
MS.gene032349.t1 AT5G41040 26.496 351 222 12 2 333 99 432 8.65e-22 96.3
MS.gene032349.t1 AT5G41040 26.496 351 222 12 2 333 115 448 1.09e-21 95.9
MS.gene032349.t1 AT1G28680 28.897 263 170 8 45 298 135 389 2.05e-21 95.1
MS.gene032349.t1 AT1G28680 31.937 191 120 5 45 228 135 322 8.96e-21 92.8
MS.gene032349.t1 AT5G63560 24.854 342 223 11 2 328 91 413 1.42e-19 89.7
MS.gene032349.t1 AT3G29590 25.484 310 206 9 48 337 138 442 1.35e-18 87.0
MS.gene032349.t1 AT3G29635 26.710 307 197 12 48 331 142 443 2.75e-17 83.2
MS.gene032349.t1 AT5G61160 25.240 313 194 11 48 333 139 438 8.75e-17 81.6
MS.gene032349.t1 AT1G24420 26.923 312 205 12 2 304 90 387 5.23e-16 79.0
MS.gene032349.t1 AT1G24420 26.923 312 205 12 2 304 90 387 5.23e-16 79.0
MS.gene032349.t1 AT5G39090 27.541 305 177 13 48 325 140 427 6.61e-16 79.0
MS.gene032349.t1 AT5G07080 26.289 194 113 4 52 226 149 331 8.60e-15 75.5
MS.gene032349.t1 AT3G03480 26.727 333 203 14 2 316 103 412 2.08e-14 74.3
MS.gene032349.t1 AT1G03390 26.857 350 215 16 5 328 110 444 2.75e-14 73.9
MS.gene032349.t1 AT5G57840 26.316 361 207 17 2 327 86 422 1.10e-13 72.0
MS.gene032349.t1 AT1G65450 25.625 320 190 13 2 295 103 400 4.67e-13 70.1
MS.gene032349.t1 AT5G39050 25.168 298 179 12 62 325 160 447 4.91e-13 70.1
MS.gene032349.t1 AT3G47170 25.128 195 123 6 52 226 153 344 9.09e-13 69.3
MS.gene032349.t1 AT1G32910 25.767 326 192 13 1 296 94 399 1.97e-12 68.6
MS.gene032349.t1 AT2G25150 24.823 282 176 12 52 312 148 414 8.04e-12 66.6
MS.gene032349.t1 AT1G78990 25.697 323 191 14 1 296 96 396 8.78e-12 66.2
MS.gene032349.t1 AT2G23510 24.138 261 173 8 53 298 150 400 1.27e-11 65.9

Find 71 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCTTGGTTTGATGTTTA+TGG 0.170784 8.2:+48621835 MS.gene032349:CDS
TGGAAATGATCCCGAGTTTA+TGG 0.213870 8.2:+48622002 MS.gene032349:CDS
CCGTTGCCTGAGGATTATTT+TGG 0.234642 8.2:+48621607 MS.gene032349:CDS
GCGAAATTCGTGCTGGATAT+TGG 0.263514 8.2:+48621562 MS.gene032349:CDS
GAGAGTATTTCATTTCAAAA+AGG 0.290139 8.2:+48621408 MS.gene032349:CDS
ATGTCTTCCTTGTGAAGTTT+TGG 0.303708 8.2:+48621972 MS.gene032349:CDS
GATGTTTATGGTAATGATTT+TGG 0.307637 8.2:+48621847 MS.gene032349:CDS
ACGATCAAATGTTGGAAGTT+TGG 0.316033 8.2:-48621287 None:intergenic
AAAATTCAAAGCAAACTTAG+AGG 0.330646 8.2:+48621447 MS.gene032349:CDS
TGGGCTTGGTAAGGGCTCTT+TGG 0.337089 8.2:+48621687 MS.gene032349:CDS
TATTGTGAAAGGAAATCTAA+TGG 0.359609 8.2:-48621335 None:intergenic
TTGAAATGAAATACTCTCTC+TGG 0.360829 8.2:-48621403 None:intergenic
GTTAACCACGTGATTGTTGA+TGG 0.384791 8.2:+48621202 MS.gene032349:CDS
CGAATTTCGCTTCTGCTTGT+GGG 0.396357 8.2:-48621549 None:intergenic
GGATTACAACCCACATTAGT+AGG 0.397014 8.2:-48622034 None:intergenic
ACGAATTTCGCTTCTGCTTG+TGG 0.401202 8.2:-48621550 None:intergenic
CATTAAAGCCGGTGACTTGT+TGG 0.413794 8.2:+48621657 MS.gene032349:CDS
TTGTTGATGGTACGTCGTTT+TGG 0.426327 8.2:+48621215 MS.gene032349:CDS
GGAAGACGGTGGGCTTGGTA+AGG 0.427310 8.2:+48621678 MS.gene032349:CDS
CCATCATGTGCAACAACAAA+GGG 0.428645 8.2:+48620993 None:intergenic
ATTGTGAAAGGAAATCTAAT+GGG 0.441170 8.2:-48621334 None:intergenic
TTAGTCGACGGCATCTTCAT+TGG 0.444242 8.2:+48621172 MS.gene032349:CDS
TTTGGCATTTCATCAATTCA+TGG 0.452731 8.2:+48621233 MS.gene032349:CDS
CACAAGGAAGACATACTTCA+AGG 0.464894 8.2:-48621963 None:intergenic
CAACTTCTCATCTGTGTGCA+AGG 0.466084 8.2:-48621728 None:intergenic
AACAGTGATTCATTGGTGAT+TGG 0.474184 8.2:+48621808 MS.gene032349:CDS
AACTTCCAACATTTGATCGT+TGG 0.480962 8.2:+48621290 MS.gene032349:CDS
TGGTGATTGGTGGTTCTCCT+TGG 0.487443 8.2:+48621821 MS.gene032349:CDS
TTGGCATTTCATCAATTCAT+GGG 0.489109 8.2:+48621234 MS.gene032349:CDS
AATGAGGATAATTATGAAGA+AGG 0.489922 8.2:+48621367 MS.gene032349:CDS
TGAATAGGAGGATGATAAGT+GGG 0.498813 8.2:-48621070 None:intergenic
CACAATAGAGTCACGAAATG+AGG 0.502975 8.2:+48621351 MS.gene032349:CDS
GTGAATAGGAGGATGATAAG+TGG 0.505672 8.2:-48621071 None:intergenic
CGTCGACTAACTCTGTTACT+TGG 0.506191 8.2:-48621159 None:intergenic
TTCAAAGCAAACTTAGAGGT+TGG 0.511060 8.2:+48621451 MS.gene032349:CDS
TTGTTGGAAGACGGTGGGCT+TGG 0.520935 8.2:+48621673 MS.gene032349:CDS
AGTGATTCATTGGTGATTGG+TGG 0.529204 8.2:+48621811 MS.gene032349:CDS
TTTCGTGACTCTATTGTGAA+AGG 0.529927 8.2:-48621346 None:intergenic
TATGGCCAACAGTGATTCAT+TGG 0.530783 8.2:+48621801 MS.gene032349:CDS
ATGAGCGAAAATTGCTTGCA+AGG 0.532407 8.2:-48621491 None:intergenic
GAAAACCAACGATCAAATGT+TGG 0.535798 8.2:-48621295 None:intergenic
GAAGACGGTGGGCTTGGTAA+GGG 0.539527 8.2:+48621679 MS.gene032349:CDS
GTGACTTGTTGGAAGACGGT+GGG 0.548009 8.2:+48621668 MS.gene032349:CDS
ACCGTCTTCCAACAAGTCAC+CGG 0.551339 8.2:-48621665 None:intergenic
AGCAGAAGCGAAATTCGTGC+TGG 0.555369 8.2:+48621555 MS.gene032349:CDS
GGAAAGATTTATGTGTATGC+GGG 0.563929 8.2:+48621919 MS.gene032349:CDS
ATACCAGAGGTGACAAAACT+CGG 0.565166 8.2:-48621781 None:intergenic
GCATTACCAAAATAATCCTC+AGG 0.566644 8.2:-48621613 None:intergenic
TGTGTTGTTAGCATTAAAGC+CGG 0.567065 8.2:+48621646 MS.gene032349:CDS
TCCATCATGTGCAACAACAA+AGG 0.573572 8.2:+48620992 None:intergenic
CCAAAATAATCCTCAGGCAA+CGG 0.577247 8.2:-48621607 None:intergenic
GTGTATGCGGGTGTAGAAGA+TGG 0.583389 8.2:+48621931 MS.gene032349:CDS
GGCAACGGAGGAATCAACCT+TGG 0.584288 8.2:-48621592 None:intergenic
ATAAGTGGGTTTAACGATGT+CGG 0.584805 8.2:-48621056 None:intergenic
GCCGGTGACTTGTTGGAAGA+CGG 0.588361 8.2:+48621664 MS.gene032349:CDS
GTTGATTCCTCCGTTGCCTG+AGG 0.590687 8.2:+48621597 MS.gene032349:CDS
GGTGACTTGTTGGAAGACGG+TGG 0.597827 8.2:+48621667 MS.gene032349:CDS
GGACACAACATCCATAAACT+CGG 0.608508 8.2:-48622013 None:intergenic
GTTACTTGGACTGCAAGTAA+CGG 0.617187 8.2:-48621145 None:intergenic
AATCACCAATGAATCACTGT+TGG 0.617756 8.2:-48621806 None:intergenic
TGGAAAGATTTATGTGTATG+CGG 0.617965 8.2:+48621918 MS.gene032349:CDS
TCATGGGCTGAAATCTCGAA+AGG 0.621563 8.2:+48621250 MS.gene032349:CDS
GACACAACATCCATAAACTC+GGG 0.639899 8.2:-48622012 None:intergenic
AAATAATCCTCAGGCAACGG+AGG 0.648191 8.2:-48621604 None:intergenic
ATCACTGTTGGCCATACCAG+AGG 0.652147 8.2:-48621794 None:intergenic
CAAGTAACAGAGTTAGTCGA+CGG 0.663446 8.2:+48621160 MS.gene032349:CDS
ATTGCCTCCAAAACTTCACA+AGG 0.673346 8.2:-48621979 None:intergenic
TTACCATAAACATCAAACCA+AGG 0.678732 8.2:-48621838 None:intergenic
TGGATATTGGAATTAGACCA+AGG 0.680206 8.2:+48621575 MS.gene032349:CDS
CATCATGTGCAACAACAAAG+GGG 0.717111 8.2:+48620994 None:intergenic
ACGTACCATCAACAATCACG+TGG 0.741961 8.2:-48621207 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAAAATCAATATGAAAAT+TGG + chr8.2:48621749-48621768 MS.gene032349:CDS 15.0%
! AAAATTCAAAGCAAACTTAG+AGG + chr8.2:48621447-48621466 MS.gene032349:CDS 25.0%
! AATGAGGATAATTATGAAGA+AGG + chr8.2:48621367-48621386 MS.gene032349:CDS 25.0%
! AATGGAGTTAAAAACTATGA+AGG + chr8.2:48621112-48621131 MS.gene032349:CDS 25.0%
! ATTGTGAAAGGAAATCTAAT+GGG - chr8.2:48621337-48621356 None:intergenic 25.0%
! TATTGTGAAAGGAAATCTAA+TGG - chr8.2:48621338-48621357 None:intergenic 25.0%
!!! AACACTAATGTTTTTTGCTA+CGG - chr8.2:48621035-48621054 None:intergenic 25.0%
!!! GAGAGTATTTCATTTCAAAA+AGG + chr8.2:48621408-48621427 MS.gene032349:CDS 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr8.2:48621847-48621866 MS.gene032349:CDS 25.0%
!!! TTATGGTAATGATTTTGGTT+GGG + chr8.2:48621852-48621871 MS.gene032349:CDS 25.0%
!!! TTTATGGTAATGATTTTGGT+TGG + chr8.2:48621851-48621870 MS.gene032349:CDS 25.0%
TGGAAAGATTTATGTGTATG+CGG + chr8.2:48621918-48621937 MS.gene032349:CDS 30.0%
TTACCATAAACATCAAACCA+AGG - chr8.2:48621841-48621860 None:intergenic 30.0%
TTGAAATGAAATACTCTCTC+TGG - chr8.2:48621406-48621425 None:intergenic 30.0%
TTGGCATTTCATCAATTCAT+GGG + chr8.2:48621234-48621253 MS.gene032349:CDS 30.0%
TTTGGCATTTCATCAATTCA+TGG + chr8.2:48621233-48621252 MS.gene032349:CDS 30.0%
! AAGCAATTTTCGCTCATATT+TGG + chr8.2:48621497-48621516 MS.gene032349:CDS 30.0%
! CATTTAGAGCAAAAAATGAG+TGG - chr8.2:48621096-48621115 None:intergenic 30.0%
!!! CACTCATTTTTTGCTCTAAA+TGG + chr8.2:48621094-48621113 MS.gene032349:CDS 30.0%
!!! TATGGTAATGATTTTGGTTG+GGG + chr8.2:48621853-48621872 MS.gene032349:CDS 30.0%
!!! TTTGATCGTTGGTTTTCAAA+AGG + chr8.2:48621301-48621320 MS.gene032349:CDS 30.0%
AAAAATGAGTGGTGAATAGG+AGG - chr8.2:48621085-48621104 None:intergenic 35.0%
AACTTCCAACATTTGATCGT+TGG + chr8.2:48621290-48621309 MS.gene032349:CDS 35.0%
AATCACCAATGAATCACTGT+TGG - chr8.2:48621809-48621828 None:intergenic 35.0%
ACGATCAAATGTTGGAAGTT+TGG - chr8.2:48621290-48621309 None:intergenic 35.0%
GAAAACCAACGATCAAATGT+TGG - chr8.2:48621298-48621317 None:intergenic 35.0%
GCAAAAAATGAGTGGTGAAT+AGG - chr8.2:48621088-48621107 None:intergenic 35.0%
GCATTACCAAAATAATCCTC+AGG - chr8.2:48621616-48621635 None:intergenic 35.0%
GGAAAGATTTATGTGTATGC+GGG + chr8.2:48621919-48621938 MS.gene032349:CDS 35.0%
TGAATAGGAGGATGATAAGT+GGG - chr8.2:48621073-48621092 None:intergenic 35.0%
TGGATATTGGAATTAGACCA+AGG + chr8.2:48621575-48621594 MS.gene032349:CDS 35.0%
TTCAAAGCAAACTTAGAGGT+TGG + chr8.2:48621451-48621470 MS.gene032349:CDS 35.0%
TTTCGTGACTCTATTGTGAA+AGG - chr8.2:48621349-48621368 None:intergenic 35.0%
! AACAGTGATTCATTGGTGAT+TGG + chr8.2:48621808-48621827 MS.gene032349:CDS 35.0%
! ATAAGTGGGTTTAACGATGT+CGG - chr8.2:48621059-48621078 None:intergenic 35.0%
! ATGTCTTCCTTGTGAAGTTT+TGG + chr8.2:48621972-48621991 MS.gene032349:CDS 35.0%
! TCTCCTTGGTTTGATGTTTA+TGG + chr8.2:48621835-48621854 MS.gene032349:CDS 35.0%
! TGTGTTGTTAGCATTAAAGC+CGG + chr8.2:48621646-48621665 MS.gene032349:CDS 35.0%
!!! ATGGTAATGATTTTGGTTGG+GGG + chr8.2:48621854-48621873 MS.gene032349:CDS 35.0%
ATACCAGAGGTGACAAAACT+CGG - chr8.2:48621784-48621803 None:intergenic 40.0%
ATGAGCGAAAATTGCTTGCA+AGG - chr8.2:48621494-48621513 None:intergenic 40.0%
ATTGCCTCCAAAACTTCACA+AGG - chr8.2:48621982-48622001 None:intergenic 40.0%
CAAGTAACAGAGTTAGTCGA+CGG + chr8.2:48621160-48621179 MS.gene032349:CDS 40.0%
CACAAGGAAGACATACTTCA+AGG - chr8.2:48621966-48621985 None:intergenic 40.0%
CACAATAGAGTCACGAAATG+AGG + chr8.2:48621351-48621370 MS.gene032349:CDS 40.0%
CCAAAATAATCCTCAGGCAA+CGG - chr8.2:48621610-48621629 None:intergenic 40.0%
GACACAACATCCATAAACTC+GGG - chr8.2:48622015-48622034 None:intergenic 40.0%
GGACACAACATCCATAAACT+CGG - chr8.2:48622016-48622035 None:intergenic 40.0%
GTGAATAGGAGGATGATAAG+TGG - chr8.2:48621074-48621093 None:intergenic 40.0%
GTTAACCACGTGATTGTTGA+TGG + chr8.2:48621202-48621221 MS.gene032349:CDS 40.0%
GTTACTTGGACTGCAAGTAA+CGG - chr8.2:48621148-48621167 None:intergenic 40.0%
TATGGCCAACAGTGATTCAT+TGG + chr8.2:48621801-48621820 MS.gene032349:CDS 40.0%
TGGAAATGATCCCGAGTTTA+TGG + chr8.2:48622002-48622021 MS.gene032349:CDS 40.0%
! AGTGATTCATTGGTGATTGG+TGG + chr8.2:48621811-48621830 MS.gene032349:CDS 40.0%
!! TCTTCCTTGTGAAGTTTTGG+AGG + chr8.2:48621975-48621994 MS.gene032349:CDS 40.0%
!!! TGTGAAGTTTTGGAGGCAAT+TGG + chr8.2:48621982-48622001 MS.gene032349:CDS 40.0%
!!! TTGTTGATGGTACGTCGTTT+TGG + chr8.2:48621215-48621234 MS.gene032349:CDS 40.0%
AAATAATCCTCAGGCAACGG+AGG - chr8.2:48621607-48621626 None:intergenic 45.0%
ACGAATTTCGCTTCTGCTTG+TGG - chr8.2:48621553-48621572 None:intergenic 45.0%
ACGTACCATCAACAATCACG+TGG - chr8.2:48621210-48621229 None:intergenic 45.0%
CAACTTCTCATCTGTGTGCA+AGG - chr8.2:48621731-48621750 None:intergenic 45.0%
CGAATTTCGCTTCTGCTTGT+GGG - chr8.2:48621552-48621571 None:intergenic 45.0%
GCGAAATTCGTGCTGGATAT+TGG + chr8.2:48621562-48621581 MS.gene032349:CDS 45.0%
TTAGTCGACGGCATCTTCAT+TGG + chr8.2:48621172-48621191 MS.gene032349:CDS 45.0%
! CATTAAAGCCGGTGACTTGT+TGG + chr8.2:48621657-48621676 MS.gene032349:CDS 45.0%
! CCGTTGCCTGAGGATTATTT+TGG + chr8.2:48621607-48621626 MS.gene032349:CDS 45.0%
! CGTCGACTAACTCTGTTACT+TGG - chr8.2:48621162-48621181 None:intergenic 45.0%
! TCATGGGCTGAAATCTCGAA+AGG + chr8.2:48621250-48621269 MS.gene032349:CDS 45.0%
!! GAGTTTTGTCACCTCTGGTA+TGG + chr8.2:48621783-48621802 MS.gene032349:CDS 45.0%
ACCGTCTTCCAACAAGTCAC+CGG - chr8.2:48621668-48621687 None:intergenic 50.0%
AGCAGAAGCGAAATTCGTGC+TGG + chr8.2:48621555-48621574 MS.gene032349:CDS 50.0%
ATCACTGTTGGCCATACCAG+AGG - chr8.2:48621797-48621816 None:intergenic 50.0%
GTGTATGCGGGTGTAGAAGA+TGG + chr8.2:48621931-48621950 MS.gene032349:CDS 50.0%
TTTGGTTGGGGGAAACCTGT+GGG + chr8.2:48621865-48621884 MS.gene032349:CDS 50.0%
! GGGGGTGCGAGTAAAAGAAA+TGG + chr8.2:48621898-48621917 MS.gene032349:CDS 50.0%
! TGGTGATTGGTGGTTCTCCT+TGG + chr8.2:48621821-48621840 MS.gene032349:CDS 50.0%
!! ACACCGAGTTTTGTCACCTC+TGG + chr8.2:48621778-48621797 MS.gene032349:CDS 50.0%
!! GTGACTTGTTGGAAGACGGT+GGG + chr8.2:48621668-48621687 MS.gene032349:CDS 50.0%
!! TTTTGGTTGGGGGAAACCTG+TGG + chr8.2:48621864-48621883 MS.gene032349:CDS 50.0%
AAACCTGTGGGGGTTCGAAG+TGG + chr8.2:48621877-48621896 MS.gene032349:CDS 55.0%
AACCTGTGGGGGTTCGAAGT+GGG + chr8.2:48621878-48621897 MS.gene032349:CDS 55.0%
GGCAACGGAGGAATCAACCT+TGG - chr8.2:48621595-48621614 None:intergenic 55.0%
TTGGTTGGGGGAAACCTGTG+GGG + chr8.2:48621866-48621885 MS.gene032349:CDS 55.0%
! GAAGACGGTGGGCTTGGTAA+GGG + chr8.2:48621679-48621698 MS.gene032349:CDS 55.0%
! GTTGATTCCTCCGTTGCCTG+AGG + chr8.2:48621597-48621616 MS.gene032349:CDS 55.0%
! TGGGCTTGGTAAGGGCTCTT+TGG + chr8.2:48621687-48621706 MS.gene032349:CDS 55.0%
! TTGTTGGAAGACGGTGGGCT+TGG + chr8.2:48621673-48621692 MS.gene032349:CDS 55.0%
!! GCCGGTGACTTGTTGGAAGA+CGG + chr8.2:48621664-48621683 MS.gene032349:CDS 55.0%
!! GGTGACTTGTTGGAAGACGG+TGG + chr8.2:48621667-48621686 MS.gene032349:CDS 55.0%
ACCTGTGGGGGTTCGAAGTG+GGG + chr8.2:48621879-48621898 MS.gene032349:CDS 60.0%
TGGTTGGGGGAAACCTGTGG+GGG + chr8.2:48621867-48621886 MS.gene032349:CDS 60.0%
! GGAAGACGGTGGGCTTGGTA+AGG + chr8.2:48621678-48621697 MS.gene032349:CDS 60.0%
CCTGTGGGGGTTCGAAGTGG+GGG + chr8.2:48621880-48621899 MS.gene032349:CDS 65.0%
CCCCCACTTCGAACCCCCAC+AGG - chr8.2:48621883-48621902 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 48620998 48622041 48620998 ID=MS.gene032349
chr8.2 mRNA 48620998 48622041 48620998 ID=MS.gene032349.t1;Parent=MS.gene032349
chr8.2 exon 48620998 48622041 48620998 ID=MS.gene032349.t1.exon1;Parent=MS.gene032349.t1
chr8.2 CDS 48620998 48622041 48620998 ID=cds.MS.gene032349.t1;Parent=MS.gene032349.t1
Gene Sequence

>MS.gene032349

ATGTGCAACAACAAAGGGGCACTCTTTGTTCATGCCGTAGCAAAAAACATTAGTGTTGCCGACATCGTTAAACCCACTTATCATCCTCCTATTCACCACTCATTTTTTGCTCTAAATGGAGTTAAAAACTATGAAGGAACATCACAACCGTTACTTGCAGTCCAAGTAACAGAGTTAGTCGACGGCATCTTCATTGGCTTCACAGTTAACCACGTGATTGTTGATGGTACGTCGTTTTGGCATTTCATCAATTCATGGGCTGAAATCTCGAAAGGTTGTCTTAAAATATCCAAACTTCCAACATTTGATCGTTGGTTTTCAAAAGGTATTGAATGTCCCATTAGATTTCCTTTCACAATAGAGTCACGAAATGAGGATAATTATGAAGAAGGAAAAATCAACTTTCCAGAGAGAGTATTTCATTTCAAAAAGGAGAGTATCGCAAAACTAAAATTCAAAGCAAACTTAGAGGTTGGTACAAAAAATATATCTTCCTTGCAAGCAATTTTCGCTCATATTTGGCGATCTATTATACGTTGCAAAAAACTTGACCCACAAGCAGAAGCGAAATTCGTGCTGGATATTGGAATTAGACCAAGGTTGATTCCTCCGTTGCCTGAGGATTATTTTGGTAATGCTGTGATAGATTGTGTTGTTAGCATTAAAGCCGGTGACTTGTTGGAAGACGGTGGGCTTGGTAAGGGCTCTTTGGAGATGAACAAGATGATTGCCTTGCACACAGATGAGAAGTTGAAAAATCAATATGAAAATTGGTTAATAACACCGAGTTTTGTCACCTCTGGTATGGCCAACAGTGATTCATTGGTGATTGGTGGTTCTCCTTGGTTTGATGTTTATGGTAATGATTTTGGTTGGGGGAAACCTGTGGGGGTTCGAAGTGGGGGTGCGAGTAAAAGAAATGGAAAGATTTATGTGTATGCGGGTGTAGAAGATGGTAGTATGAACCTTGAAGTATGTCTTCCTTGTGAAGTTTTGGAGGCAATTGGAAATGATCCCGAGTTTATGGATGTTGTGTCCTACTAA
Protein sequence

>MS.gene032349.t1

MCNNKGALFVHAVAKNISVADIVKPTYHPPIHHSFFALNGVKNYEGTSQPLLAVQVTELVDGIFIGFTVNHVIVDGTSFWHFINSWAEISKGCLKISKLPTFDRWFSKGIECPIRFPFTIESRNEDNYEEGKINFPERVFHFKKESIAKLKFKANLEVGTKNISSLQAIFAHIWRSIIRCKKLDPQAEAKFVLDIGIRPRLIPPLPEDYFGNAVIDCVVSIKAGDLLEDGGLGKGSLEMNKMIALHTDEKLKNQYENWLITPSFVTSGMANSDSLVIGGSPWFDVYGNDFGWGKPVGVRSGGASKRNGKIYVYAGVEDGSMNLEVCLPCEVLEAIGNDPEFMDVVSY