AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99221

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99221.t1 MTR_4g094835 68.653 453 131 5 1 448 1 447 0.0 629
MS.gene99221.t1 MTR_4g094838 66.004 453 124 5 4 447 1 432 0.0 588
MS.gene99221.t1 MTR_4g094842 58.499 453 169 8 1 443 1 444 0.0 526
MS.gene99221.t1 MTR_4g094832 67.539 382 104 4 78 447 57 430 0.0 521
MS.gene99221.t1 MTR_0190s0020 57.965 452 175 8 1 447 1 442 6.66e-179 508
MS.gene99221.t1 MTR_8g041770 55.973 452 183 9 1 447 1 441 1.80e-172 491
MS.gene99221.t1 MTR_4g094828 54.664 461 186 11 1 448 1 451 1.07e-171 489
MS.gene99221.t1 MTR_3g088420 56.570 449 177 10 1 447 1 433 5.69e-170 484
MS.gene99221.t1 MTR_4g022825 54.767 451 183 11 1 444 1 437 6.77e-166 474
MS.gene99221.t1 MTR_8g079710 56.696 448 172 11 1 438 1 436 3.61e-165 473
MS.gene99221.t1 MTR_4g022800 54.705 457 182 11 1 447 1 442 3.72e-164 470
MS.gene99221.t1 MTR_4g022290 54.825 456 180 11 1 444 1 442 5.28e-164 470
MS.gene99221.t1 MTR_4g022720 54.425 452 184 10 1 447 1 435 7.88e-163 466
MS.gene99221.t1 MTR_4g022670 53.422 453 187 11 1 444 1 438 7.03e-161 462
MS.gene99221.t1 MTR_3g026310 53.159 459 186 12 1 444 1 445 4.10e-159 457
MS.gene99221.t1 MTR_7g026860 53.626 455 186 12 1 447 1 438 3.72e-157 452
MS.gene99221.t1 MTR_4g022760 48.132 455 170 10 1 447 1 397 2.39e-135 395
MS.gene99221.t1 MTR_6g016815 42.411 448 176 12 1 447 15 381 5.31e-102 309
MS.gene99221.t1 MTR_3g101900 39.767 430 243 7 27 447 26 448 2.94e-96 297
MS.gene99221.t1 MTR_3g102020 39.394 462 250 11 3 448 5 452 7.91e-95 293
MS.gene99221.t1 MTR_3g101930 39.140 442 243 8 21 444 19 452 1.65e-93 290
MS.gene99221.t1 MTR_3g102010 38.696 460 253 11 4 448 7 452 4.17e-91 284
MS.gene99221.t1 MTR_3g101960 38.178 461 258 9 3 449 5 452 8.14e-91 283
MS.gene99221.t1 MTR_3g101970 38.551 428 235 8 27 444 25 434 1.21e-88 277
MS.gene99221.t1 MTR_5g081960 34.244 476 244 10 24 443 20 482 1.94e-81 260
MS.gene99221.t1 MTR_4g094825 76.301 173 36 3 19 188 80 250 2.02e-81 257
MS.gene99221.t1 MTR_4g094825 76.000 150 26 1 298 447 252 391 4.32e-70 228
MS.gene99221.t1 MTR_3g070170 35.294 459 240 10 24 443 26 466 3.15e-80 256
MS.gene99221.t1 MTR_8g040780 55.714 210 88 3 236 442 20 227 7.49e-74 233
MS.gene99221.t1 MTR_4g015180 32.794 494 260 9 8 443 4 483 9.52e-73 237
MS.gene99221.t1 MTR_8g040760 53.881 219 94 5 1 217 1 214 4.98e-68 216
MS.gene99221.t1 MTR_3g102030 32.938 337 183 7 75 401 17 320 1.02e-49 172
MS.gene99221.t1 MTR_7g026850 50.588 170 79 4 36 203 12 178 1.07e-47 162
MS.gene99221.t1 MTR_7g026770 48.619 181 70 3 151 331 132 289 2.73e-47 165
MS.gene99221.t1 MTR_6g045553 28.982 452 275 12 27 443 31 471 7.64e-47 168
MS.gene99221.t1 MTR_6g034485 53.846 130 55 2 196 325 1 125 3.17e-42 146
MS.gene99221.t1 MTR_6g034495 52.817 142 58 3 56 188 19 160 2.41e-41 145
MS.gene99221.t1 MTR_8g075610 27.949 390 241 17 64 436 47 413 1.42e-30 122
MS.gene99221.t1 MTR_4g007540 30.357 392 237 19 64 438 56 428 2.48e-30 122
MS.gene99221.t1 MTR_8g096530 31.105 344 206 13 64 396 59 382 7.16e-29 118
MS.gene99221.t1 MTR_3g098980 27.688 372 243 11 64 428 66 418 4.76e-27 113
MS.gene99221.t1 MTR_7g026830 58.537 82 34 0 94 175 16 97 6.86e-27 103
MS.gene99221.t1 MTR_2g105330 27.937 383 237 19 64 428 56 417 1.08e-26 112
MS.gene99221.t1 MTR_2g064495 27.937 383 237 19 64 428 56 417 2.29e-26 110
MS.gene99221.t1 MTR_3g026950 25.123 406 251 13 61 432 51 437 2.56e-25 108
MS.gene99221.t1 MTR_3g049150 24.557 395 262 12 61 432 52 433 1.10e-24 106
MS.gene99221.t1 MTR_4g127650 24.601 439 287 15 26 436 26 448 2.35e-24 105
MS.gene99221.t1 MTR_7g027175 27.604 384 253 10 59 434 63 429 2.63e-24 105
MS.gene99221.t1 MTR_4g097090 24.434 442 265 18 28 428 27 440 2.31e-23 102
MS.gene99221.t1 MTR_3g071320 27.103 321 206 10 29 333 24 332 9.73e-21 94.7
MS.gene99221.t1 MTR_7g026780 43.750 112 37 2 336 447 26 111 2.59e-20 86.3
MS.gene99221.t1 MTR_1g081660 23.174 397 266 13 31 409 31 406 1.57e-18 88.2
MS.gene99221.t1 MTR_1g040175 23.529 408 272 17 61 450 61 446 1.58e-18 88.2
MS.gene99221.t1 MTR_6g015850 24.353 464 300 13 3 436 7 449 1.68e-18 88.2
MS.gene99221.t1 MTR_3g087640 27.753 454 260 23 29 434 29 462 2.91e-18 87.4
MS.gene99221.t1 MTR_8g012750 24.390 410 257 15 29 396 24 422 3.66e-18 87.0
MS.gene99221.t1 MTR_6g015315 25.208 361 231 12 63 399 62 407 4.64e-18 86.7
MS.gene99221.t1 MTR_7g014330 26.173 405 247 18 31 400 26 413 6.84e-18 86.3
MS.gene99221.t1 MTR_7g056087 25.773 388 247 18 62 429 56 422 1.43e-17 85.1
MS.gene99221.t1 MTR_7g084940 25.797 345 223 11 64 396 69 392 3.88e-17 84.0
MS.gene99221.t1 MTR_6g015830 23.656 465 305 15 3 436 7 452 5.72e-17 83.2
MS.gene99221.t1 MTR_4g013050 24.863 366 200 13 58 397 47 363 6.58e-17 82.8
MS.gene99221.t1 MTR_1g040195 25.349 430 247 21 32 434 23 405 8.27e-17 82.8
MS.gene99221.t1 MTR_7g114470 22.706 436 296 13 32 443 25 443 9.19e-17 82.8
MS.gene99221.t1 MTR_7g086000 27.363 402 255 16 62 436 58 449 1.12e-16 82.4
MS.gene99221.t1 MTR_3g112180 25.806 279 189 8 60 329 57 326 1.52e-16 82.0
MS.gene99221.t1 MTR_6g015320 25.281 356 236 11 63 399 65 409 1.59e-16 82.0
MS.gene99221.t1 MTR_1g053315 23.362 351 234 15 64 397 78 410 1.64e-16 82.0
MS.gene99221.t1 MTR_4g055170 26.781 351 227 11 64 399 69 404 3.26e-16 80.9
MS.gene99221.t1 MTR_2g015430 24.932 369 246 12 40 399 40 386 6.30e-16 80.1
MS.gene99221.t1 MTR_3g049140 27.230 213 142 5 231 432 20 230 6.84e-16 77.8
MS.gene99221.t1 MTR_7g014250 23.649 444 288 17 29 438 24 450 7.14e-16 80.1
MS.gene99221.t1 MTR_7g085930 25.269 372 236 13 64 402 59 421 7.19e-16 80.1
MS.gene99221.t1 MTR_5g076700 22.759 435 296 13 32 443 24 441 9.01e-16 79.7
MS.gene99221.t1 MTR_3g028210 25.602 457 292 19 4 428 8 448 2.14e-15 78.6
MS.gene99221.t1 MTR_3g465980 25.424 295 203 7 59 344 63 349 4.68e-15 77.4
MS.gene99221.t1 MTR_2g089755 26.190 378 219 17 64 399 73 432 5.79e-15 77.4
MS.gene99221.t1 MTR_2g089765 23.874 444 284 18 29 431 37 467 7.47e-15 77.0
MS.gene99221.t1 MTR_1g094790 26.000 450 261 23 39 449 32 448 2.06e-14 75.5
MS.gene99221.t1 MTR_7g114490 22.664 428 286 15 32 434 24 431 4.37e-14 74.7
MS.gene99221.t1 MTR_8g079980 22.753 356 240 11 59 402 54 386 4.91e-14 74.3
MS.gene99221.t1 MTR_7g015860 25.623 281 165 9 147 397 147 413 9.00e-14 73.6
MS.gene99221.t1 MTR_7g014230 23.844 411 253 16 29 400 26 415 9.90e-14 73.2
MS.gene99221.t1 MTR_3g465880 24.746 295 210 7 58 344 64 354 1.24e-13 73.2
MS.gene99221.t1 MTR_5g084850 23.056 360 235 15 62 408 57 387 3.11e-13 71.6
MS.gene99221.t1 MTR_1g040215 22.917 384 259 15 32 397 23 387 6.44e-13 70.9
MS.gene99221.t1 MTR_7g114550 20.920 435 303 13 32 443 24 440 8.44e-13 70.5
MS.gene99221.t1 MTR_3g465990 24.296 284 187 7 59 329 63 331 1.09e-12 70.1
MS.gene99221.t1 MTR_7g114560 24.149 323 209 11 32 332 24 332 1.22e-12 70.1
MS.gene99221.t1 MTR_7g114520 21.789 436 300 14 32 443 25 443 1.40e-12 69.7
MS.gene99221.t1 MTR_7g114960 24.752 404 264 18 61 443 60 444 1.98e-12 69.3
MS.gene99221.t1 MTR_2g089650 24.041 391 255 17 64 424 65 443 2.70e-12 68.9
MS.gene99221.t1 MTR_7g014160 23.821 424 251 16 49 428 60 455 2.83e-12 68.9
MS.gene99221.t1 MTR_7g026880 56.863 51 20 1 394 444 2 50 3.19e-12 61.6
MS.gene99221.t1 MTR_7g026870 43.662 71 34 2 291 355 81 151 4.70e-12 64.3
MS.gene99221.t1 MTR_1g040315 24.679 312 199 12 32 323 23 318 5.30e-12 67.8
MS.gene99221.t1 MTR_8g467580 24.944 449 275 19 23 443 18 432 7.17e-12 67.4
MS.gene99221.t1 MTR_0502s0010 27.136 199 123 7 147 332 42 231 3.13e-11 63.5
MS.gene99221.t1 MTR_5g090810 22.362 398 249 15 39 397 36 412 4.80e-11 65.1
MS.gene99221.t1 MTR_7g084910 25.746 268 162 10 64 313 70 318 6.38e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99221.t1 AT5G67150 41.111 450 250 7 1 443 1 442 9.70e-113 339
MS.gene99221.t1 AT3G50280 37.111 450 271 6 1 446 1 442 5.55e-102 311
MS.gene99221.t1 AT3G50300 38.731 457 262 8 1 448 1 448 4.46e-101 309
MS.gene99221.t1 AT3G50270 39.052 443 262 6 6 444 7 445 2.61e-99 305
MS.gene99221.t1 AT5G67160 39.243 423 230 9 27 442 25 427 1.88e-93 289
MS.gene99221.t1 AT5G38130 38.249 434 252 7 1 429 21 443 2.34e-93 290
MS.gene99221.t1 AT5G42830 35.280 428 258 8 27 443 26 445 7.88e-84 265
MS.gene99221.t1 AT2G39980 33.983 462 246 6 29 443 27 476 3.89e-83 264
MS.gene99221.t1 AT5G07850 36.952 433 251 9 31 449 31 455 1.63e-80 256
MS.gene99221.t1 AT5G07860 35.897 429 245 9 31 443 31 445 7.88e-79 252
MS.gene99221.t1 AT5G01210 34.061 458 252 7 24 443 20 465 7.74e-76 245
MS.gene99221.t1 AT3G50280 38.413 315 188 3 135 446 1 312 1.63e-75 239
MS.gene99221.t1 AT5G07870 35.360 444 251 9 31 449 31 463 8.32e-75 242
MS.gene99221.t1 AT5G23940 31.954 435 271 11 27 443 21 448 9.25e-61 206
MS.gene99221.t1 AT3G50290 39.925 268 154 4 132 394 4 269 1.22e-59 196
MS.gene99221.t1 AT5G48930 29.058 382 241 16 64 431 56 421 1.55e-27 114
MS.gene99221.t1 AT5G41040 28.418 373 242 12 61 424 66 422 2.10e-26 111
MS.gene99221.t1 AT5G41040 28.418 373 242 12 61 424 82 438 2.29e-26 111
MS.gene99221.t1 AT3G48720 27.877 391 244 17 62 436 59 427 3.99e-25 107
MS.gene99221.t1 AT2G40230 25.362 414 285 8 1 407 1 397 4.42e-24 104
MS.gene99221.t1 AT3G29590 25.408 429 301 9 23 438 20 442 8.80e-24 103
MS.gene99221.t1 AT1G78990 28.184 369 219 14 51 397 52 396 1.37e-23 103
MS.gene99221.t1 AT1G28680 26.590 346 226 10 64 399 62 389 1.92e-22 99.8
MS.gene99221.t1 AT2G19070 24.198 405 266 13 59 436 53 443 3.29e-22 99.0
MS.gene99221.t1 AT5G63560 26.649 379 237 14 64 428 61 412 6.30e-22 98.2
MS.gene99221.t1 AT1G28680 26.871 294 196 7 67 353 65 346 5.58e-21 94.7
MS.gene99221.t1 AT1G24430 26.486 370 229 15 62 420 60 397 4.82e-20 92.4
MS.gene99221.t1 AT3G03480 26.914 405 250 18 32 416 33 411 9.90e-19 88.6
MS.gene99221.t1 AT3G29670 25.877 456 284 20 3 428 2 433 4.64e-18 86.7
MS.gene99221.t1 AT5G57840 25.296 423 244 19 44 428 34 422 9.93e-18 85.5
MS.gene99221.t1 AT5G39090 25.909 440 276 22 19 431 16 432 2.44e-17 84.3
MS.gene99221.t1 AT3G30280 24.667 450 264 19 32 443 20 432 5.59e-17 83.2
MS.gene99221.t1 AT5G16410 23.596 356 227 12 62 397 89 419 1.74e-16 82.0
MS.gene99221.t1 AT5G39080 22.609 460 302 16 14 436 11 453 1.92e-16 81.6
MS.gene99221.t1 AT3G26040 24.627 402 254 16 60 443 55 425 2.00e-16 81.6
MS.gene99221.t1 AT5G07080 23.611 432 283 13 40 448 43 450 3.19e-16 80.9
MS.gene99221.t1 AT1G31490 24.685 397 261 15 61 442 59 432 4.38e-16 80.5
MS.gene99221.t1 AT5G17540 24.041 391 261 13 32 405 23 394 6.26e-16 80.1
MS.gene99221.t1 AT5G39050 23.175 315 207 10 145 431 145 452 1.29e-14 76.3
MS.gene99221.t1 AT2G23510 23.188 345 243 9 64 399 69 400 1.99e-14 75.5
MS.gene99221.t1 AT5G47950 24.419 430 273 17 32 443 20 415 7.34e-14 73.6
MS.gene99221.t1 AT3G47170 23.490 298 206 8 57 335 58 352 9.43e-14 73.6
MS.gene99221.t1 AT1G03390 26.836 354 223 16 61 397 73 407 2.29e-13 72.4
MS.gene99221.t1 AT3G29635 24.352 386 257 14 64 428 67 438 5.75e-13 70.9
MS.gene99221.t1 AT5G61160 24.632 272 179 10 145 397 139 403 2.23e-12 69.3
MS.gene99221.t1 AT5G23970 24.581 358 218 15 61 399 51 375 9.41e-12 67.0
MS.gene99221.t1 AT1G03495 26.990 289 180 12 59 323 61 342 1.56e-11 65.9

Find 110 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCTTTCTTATGAGATTT+TGG 0.242506 8.4:+26237685 MS.gene99221:CDS
TGTTCCACTTCCAAGTTCTA+TGG 0.245627 8.4:+26237743 MS.gene99221:CDS
TTCCGAAATAATCCTCTTCT+AGG 0.269532 8.4:-26237322 None:intergenic
GGATTCTAAGCTTTCTTCTA+AGG 0.272080 8.4:-26237081 None:intergenic
GGGATTGGTAACAAGTATAA+TGG 0.290220 8.4:+26237611 MS.gene99221:CDS
TTGGTATTGGAATGGGTTGT+TGG 0.293902 8.4:-26236560 None:intergenic
GTTTCTTATGTGTTGTTTAT+AGG 0.297168 8.4:+26237275 MS.gene99221:CDS
GCAGGAGAGGTTCTAGAGTA+TGG 0.316001 8.4:+26237377 MS.gene99221:CDS
GGGGAATGACACTGAGTTCA+TGG 0.325881 8.4:+26237715 MS.gene99221:CDS
TACATTCTGATAAGATAATA+AGG 0.327777 8.4:+26237441 MS.gene99221:CDS
TTTGTTTAAGATGGTTTATA+TGG 0.335226 8.4:-26236584 None:intergenic
TTGCAGACGGCAAGTCGTTT+TGG 0.346102 8.4:+26236916 MS.gene99221:CDS
GAAACATCTTCTTGTGGATC+AGG 0.367065 8.4:-26237257 None:intergenic
CCCCTAGAAGAGGATTATTT+CGG 0.368115 8.4:+26237320 MS.gene99221:CDS
TGGTTTCAAGATGGAGGAAT+TGG 0.375248 8.4:-26236515 None:intergenic
TTGGAATTTCAATATCGTTT+GGG 0.375437 8.4:-26237016 None:intergenic
TATATGGTTTGGTATTGGAA+TGG 0.381013 8.4:-26236568 None:intergenic
AAAGATTCTTTCGGCTAACT+TGG 0.384070 8.4:-26237102 None:intergenic
TGGTTTATATGGTTTGGTAT+TGG 0.385182 8.4:-26236573 None:intergenic
AAGCGCTATTATCTCACGTT+TGG 0.385318 8.4:+26237210 MS.gene99221:CDS
TGTGAAATGAAAGATTCTTT+CGG 0.395295 8.4:-26237111 None:intergenic
TTAAGATGGTTTATATGGTT+TGG 0.398195 8.4:-26236579 None:intergenic
ATTGGAATTTCAATATCGTT+TGG 0.400486 8.4:-26237017 None:intergenic
AGCTTTCTTCTAAGGAGCCT+TGG 0.410094 8.4:-26237073 None:intergenic
TTTGGCATTTCGTTAATTCA+TGG 0.411811 8.4:+26236934 MS.gene99221:CDS
TTGTGATTGTTGTTGTCAAT+TGG 0.414221 8.4:-26236450 None:intergenic
AGGAAAGTGTTTGTTTAAGA+TGG 0.419599 8.4:-26236593 None:intergenic
TTGGTTGTGACACAGCTTCA+TGG 0.433925 8.4:-26236827 None:intergenic
TTAAGTGACGGCGTCTTCAT+TGG 0.436912 8.4:+26236873 MS.gene99221:CDS
TAAGGAATCATTACGAGTCT+TGG 0.444628 8.4:+26237459 MS.gene99221:CDS
ATATGGTTTGGTATTGGAAT+GGG 0.445755 8.4:-26236567 None:intergenic
TCAAAAGCAAACGCCGAAGC+TGG 0.446381 8.4:+26237158 MS.gene99221:CDS
GAGGTGTATAGTTTGATTGA+AGG 0.450138 8.4:-26236764 None:intergenic
ACTGGTGGAGATCACTTTGA+TGG 0.452170 8.4:-26236415 None:intergenic
AACTTCTTTCCACCCCTCGC+TGG 0.454179 8.4:+26236624 MS.gene99221:CDS
TCCGAAATAATCCTCTTCTA+GGG 0.454875 8.4:-26237321 None:intergenic
TGGAATCGGAAAAGTTGCTT+TGG 0.455281 8.4:+26237397 MS.gene99221:CDS
TTTGTGAAAGGGAAGTGTAT+TGG 0.457281 8.4:-26237035 None:intergenic
GGATTGGTAACAAGTATAAT+GGG 0.461227 8.4:+26237612 MS.gene99221:CDS
GCATCATTTCCCATAGAACT+TGG 0.464131 8.4:-26237753 None:intergenic
GGGAAAATTACAGTGTTTGC+TGG 0.464637 8.4:+26237632 MS.gene99221:CDS
GGGAACCAACGTTGAAATGA+AGG 0.474068 8.4:-26236996 None:intergenic
AATTGGTGCTTGAATTGTAC+TGG 0.484057 8.4:-26236433 None:intergenic
GAAAAGAAGGCCTGCTTGGA+TGG 0.486070 8.4:-26236535 None:intergenic
ATCCCACTTCGAACTGCCAC+AGG 0.501391 8.4:-26237593 None:intergenic
TGGGTTGTTGGTGGAAAAGA+AGG 0.501427 8.4:-26236548 None:intergenic
TCATGGTCACAAATCTCAAA+AGG 0.504161 8.4:+26236951 MS.gene99221:CDS
TGGAAAGAAGTTGAGGGTGG+AGG 0.509184 8.4:-26236613 None:intergenic
GTTCCACTTCCAAGTTCTAT+GGG 0.509409 8.4:+26237744 MS.gene99221:CDS
GTGGCAGTTCGAAGTGGGAT+TGG 0.513271 8.4:+26237596 MS.gene99221:CDS
GTATTGGAATGGGTTGTTGG+TGG 0.516749 8.4:-26236557 None:intergenic
TCCATCATTTGCAACAACCT+TGG 0.522468 8.4:+26236693 MS.gene99221:CDS
CCACTTGGAGGACTACCACT+TGG 0.530738 8.4:-26237503 None:intergenic
ACCAAGGTTGTTGCAAATGA+TGG 0.532824 8.4:-26236694 None:intergenic
TTTCCCATAGAACTTGGAAG+TGG 0.534045 8.4:-26237747 None:intergenic
GAGGTTCTAGAGTATGGAAT+CGG 0.537759 8.4:+26237383 MS.gene99221:CDS
TAGCAATCACTTGTTGTCCA+AGG 0.538515 8.4:+26237534 MS.gene99221:CDS
CTGCTTGGATGGTTTCAAGA+TGG 0.545156 8.4:-26236524 None:intergenic
AACTCCCTTCATTTCAACGT+TGG 0.545872 8.4:+26236991 MS.gene99221:CDS
GAAAATTGAATTAACACCAT+GGG 0.549693 8.4:+26236488 MS.gene99221:CDS
GGTGGAAAAGAAGGCCTGCT+TGG 0.549803 8.4:-26236539 None:intergenic
CGTCACTTAACTCCGTTACC+TGG 0.550388 8.4:-26236860 None:intergenic
GCTAACGAATTACCACTTGG+AGG 0.558087 8.4:-26237515 None:intergenic
CGAGGGGTGGAAAGAAGTTG+AGG 0.560871 8.4:-26236620 None:intergenic
CATCTTGAAACCATCCAAGC+AGG 0.563003 8.4:+26236525 MS.gene99221:CDS
ACAACCAACTCTAGGAGTCC+AGG 0.567424 8.4:+26236842 MS.gene99221:CDS
TCTAGGGGCGGAATCAACCT+TGG 0.569302 8.4:-26237305 None:intergenic
TGCAACCACGTTGTTGCAGA+CGG 0.570748 8.4:+26236903 MS.gene99221:CDS
GTGTTTGCTGGTGCTGAAGA+AGG 0.574224 8.4:+26237644 MS.gene99221:CDS
GGAACCAACGTTGAAATGAA+GGG 0.576282 8.4:-26236995 None:intergenic
CTACCACTTGGAAACAACCT+TGG 0.580849 8.4:-26237491 None:intergenic
GTTGATTCCGCCCCTAGAAG+AGG 0.580936 8.4:+26237310 MS.gene99221:CDS
AGCCTGTGGCAGTTCGAAGT+GGG 0.585379 8.4:+26237591 MS.gene99221:CDS
GGGTGGAAAGAAGTTGAGGG+TGG 0.587080 8.4:-26236616 None:intergenic
GCTGTGTCACAACCAACTCT+AGG 0.587882 8.4:+26236834 MS.gene99221:CDS
CTTGGATGGTTTCAAGATGG+AGG 0.589594 8.4:-26236521 None:intergenic
TAGAACTTGGAAGTGGAACA+TGG 0.589628 8.4:-26237740 None:intergenic
ACATAAGAAACATCTTCTTG+TGG 0.596961 8.4:-26237263 None:intergenic
CCGAAATAATCCTCTTCTAG+GGG 0.597381 8.4:-26237320 None:intergenic
AGTGCAAAATACAACGGTCG+TGG 0.601343 8.4:+26236737 MS.gene99221:CDS
TGGAGGAATTGGAGATCCCA+TGG 0.604811 8.4:-26236504 None:intergenic
AACTCTAGGAGTCCAGGTAA+CGG 0.604904 8.4:+26236848 MS.gene99221:CDS
AAGAATCTTTCATTTCACAA+AGG 0.609560 8.4:+26237115 MS.gene99221:CDS
GTTACCTGGACTCCTAGAGT+TGG 0.609941 8.4:-26236846 None:intergenic
AGAAAATTGAATTAACACCA+TGG 0.610904 8.4:+26236487 MS.gene99221:CDS
TGTTTATAGGTGTTAGACCA+AGG 0.611467 8.4:+26237288 MS.gene99221:CDS
GAGGGGTGGAAAGAAGTTGA+GGG 0.618695 8.4:-26236619 None:intergenic
ACAAGACGACCAGCGAGGGG+TGG 0.619470 8.4:-26236633 None:intergenic
AAGCCTGTGGCAGTTCGAAG+TGG 0.622477 8.4:+26237590 MS.gene99221:CDS
ACTGTTGTGAGGATGAAAGC+AGG 0.624888 8.4:+26237359 MS.gene99221:CDS
TATTACAAGACGACCAGCGA+GGG 0.630336 8.4:-26236637 None:intergenic
AAATAATCCTCTTCTAGGGG+CGG 0.632202 8.4:-26237317 None:intergenic
GGATTATTTCGGAAACGCAG+TGG 0.636378 8.4:+26237331 MS.gene99221:CDS
TTATTTCGGAAACGCAGTGG+TGG 0.651714 8.4:+26237334 MS.gene99221:CDS
ATTGCTAACGAATTACCACT+TGG 0.655593 8.4:-26237518 None:intergenic
TGGTGCTTGAATTGTACTGG+TGG 0.661054 8.4:-26236430 None:intergenic
TGTGAGGATGAAAGCAGGAG+AGG 0.662636 8.4:+26237364 MS.gene99221:CDS
ACACCAAGGTTGTTTCCAAG+TGG 0.665409 8.4:+26237488 MS.gene99221:CDS
AAAACAGAATCACAAAACCA+AGG 0.668777 8.4:+26237056 MS.gene99221:CDS
ATTACAAGACGACCAGCGAG+GGG 0.669009 8.4:-26236636 None:intergenic
CAGGTAACGGAGTTAAGTGA+CGG 0.674400 8.4:+26236861 MS.gene99221:CDS
TTTCACAAAGGAGAAAATCG+CGG 0.682651 8.4:+26237127 MS.gene99221:CDS
CAGTGGTGGTTACTGTTGTG+AGG 0.691041 8.4:+26237348 MS.gene99221:CDS
GTTGCAAATGATGGAACATG+TGG 0.705405 8.4:-26236685 None:intergenic
CCAAGTGGTAGTCCTCCAAG+TGG 0.727113 8.4:+26237503 MS.gene99221:CDS
TGCCACAGTGCAAAATACAA+CGG 0.729193 8.4:+26236731 MS.gene99221:CDS
ACTTGCCGTCTGCAACAACG+TGG 0.730274 8.4:-26236908 None:intergenic
CATGGACGAATAGTGCACCA+AGG 0.738412 8.4:-26236710 None:intergenic
TTATTACAAGACGACCAGCG+AGG 0.755763 8.4:-26236638 None:intergenic
AGTCTTGGTTAAGAACACCA+AGG 0.764465 8.4:+26237474 MS.gene99221:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACATTCTGATAAGATAATA+AGG + chr8.4:26237441-26237460 MS.gene99221:CDS 20.0%
!!! TTTGTTTAAGATGGTTTATA+TGG - chr8.4:26236587-26236606 None:intergenic 20.0%
! AAGAATCTTTCATTTCACAA+AGG + chr8.4:26237115-26237134 MS.gene99221:CDS 25.0%
! AGAAAATTGAATTAACACCA+TGG + chr8.4:26236487-26236506 MS.gene99221:CDS 25.0%
! ATTGGAATTTCAATATCGTT+TGG - chr8.4:26237020-26237039 None:intergenic 25.0%
! GAAAATTGAATTAACACCAT+GGG + chr8.4:26236488-26236507 MS.gene99221:CDS 25.0%
! TGTGAAATGAAAGATTCTTT+CGG - chr8.4:26237114-26237133 None:intergenic 25.0%
! TTAAGATGGTTTATATGGTT+TGG - chr8.4:26236582-26236601 None:intergenic 25.0%
! TTGGAATTTCAATATCGTTT+GGG - chr8.4:26237019-26237038 None:intergenic 25.0%
!! TTGTCTTTCTTATGAGATTT+TGG + chr8.4:26237685-26237704 MS.gene99221:CDS 25.0%
!!! GTTTCTTATGTGTTGTTTAT+AGG + chr8.4:26237275-26237294 MS.gene99221:CDS 25.0%
AAAACAGAATCACAAAACCA+AGG + chr8.4:26237056-26237075 MS.gene99221:CDS 30.0%
ACATAAGAAACATCTTCTTG+TGG - chr8.4:26237266-26237285 None:intergenic 30.0%
TTTGGCATTTCGTTAATTCA+TGG + chr8.4:26236934-26236953 MS.gene99221:CDS 30.0%
! GGATTGGTAACAAGTATAAT+GGG + chr8.4:26237612-26237631 MS.gene99221:CDS 30.0%
! TCTTTCTTATGAGATTTTGG+AGG + chr8.4:26237688-26237707 MS.gene99221:CDS 30.0%
! TTGTGATTGTTGTTGTCAAT+TGG - chr8.4:26236453-26236472 None:intergenic 30.0%
!! AAGGGAGTTTTGATATTTTG+TGG - chr8.4:26236980-26236999 None:intergenic 30.0%
!! AGGAAAGTGTTTGTTTAAGA+TGG - chr8.4:26236596-26236615 None:intergenic 30.0%
!! AGGGAGTTTTGATATTTTGT+GGG - chr8.4:26236979-26236998 None:intergenic 30.0%
!! ATATGGTTTGGTATTGGAAT+GGG - chr8.4:26236570-26236589 None:intergenic 30.0%
!! TATATGGTTTGGTATTGGAA+TGG - chr8.4:26236571-26236590 None:intergenic 30.0%
!! TGGTTTATATGGTTTGGTAT+TGG - chr8.4:26236576-26236595 None:intergenic 30.0%
!! TTTTTGGCAATGATTTTGGT+TGG + chr8.4:26237564-26237583 MS.gene99221:CDS 30.0%
!!! GATGTTTTTGGCAATGATTT+TGG + chr8.4:26237560-26237579 MS.gene99221:CDS 30.0%
!!! GTGATTCTGTTTTTGTGAAA+GGG - chr8.4:26237049-26237068 None:intergenic 30.0%
!!! TGTGATTCTGTTTTTGTGAA+AGG - chr8.4:26237050-26237069 None:intergenic 30.0%
!!! TTTTGGCAATGATTTTGGTT+GGG + chr8.4:26237565-26237584 MS.gene99221:CDS 30.0%
AAAAATGAGTGGACAATTGG+AGG - chr8.4:26236786-26236805 None:intergenic 35.0%
AAAGATTCTTTCGGCTAACT+TGG - chr8.4:26237105-26237124 None:intergenic 35.0%
ATTGCTAACGAATTACCACT+TGG - chr8.4:26237521-26237540 None:intergenic 35.0%
GAGGTGTATAGTTTGATTGA+AGG - chr8.4:26236767-26236786 None:intergenic 35.0%
GGAAAAAATGAGTGGACAAT+TGG - chr8.4:26236789-26236808 None:intergenic 35.0%
GGATTCTAAGCTTTCTTCTA+AGG - chr8.4:26237084-26237103 None:intergenic 35.0%
GGTTAAGTGGAAAAAATGAG+TGG - chr8.4:26236797-26236816 None:intergenic 35.0%
TAAGGAATCATTACGAGTCT+TGG + chr8.4:26237459-26237478 MS.gene99221:CDS 35.0%
TCATGGTCACAAATCTCAAA+AGG + chr8.4:26236951-26236970 MS.gene99221:CDS 35.0%
TCCGAAATAATCCTCTTCTA+GGG - chr8.4:26237324-26237343 None:intergenic 35.0%
TGTTTATAGGTGTTAGACCA+AGG + chr8.4:26237288-26237307 MS.gene99221:CDS 35.0%
TTCCGAAATAATCCTCTTCT+AGG - chr8.4:26237325-26237344 None:intergenic 35.0%
TTGCCAAAAACATCAAACCT+TGG - chr8.4:26237554-26237573 None:intergenic 35.0%
TTTCACAAAGGAGAAAATCG+CGG + chr8.4:26237127-26237146 MS.gene99221:CDS 35.0%
! AATTGGTGCTTGAATTGTAC+TGG - chr8.4:26236436-26236455 None:intergenic 35.0%
! GGGATTGGTAACAAGTATAA+TGG + chr8.4:26237611-26237630 MS.gene99221:CDS 35.0%
! TGTCCAAGGTTTGATGTTTT+TGG + chr8.4:26237548-26237567 MS.gene99221:CDS 35.0%
! TTTGTGAAAGGGAAGTGTAT+TGG - chr8.4:26237038-26237057 None:intergenic 35.0%
!! TATGAGATTTTGGAGGCTTT+GGG + chr8.4:26237695-26237714 MS.gene99221:CDS 35.0%
!! TTATGAGATTTTGGAGGCTT+TGG + chr8.4:26237694-26237713 MS.gene99221:CDS 35.0%
!!! TTTGGCAATGATTTTGGTTG+GGG + chr8.4:26237566-26237585 MS.gene99221:CDS 35.0%
AACTCCCTTCATTTCAACGT+TGG + chr8.4:26236991-26237010 MS.gene99221:CDS 40.0%
AAGCGCTATTATCTCACGTT+TGG + chr8.4:26237210-26237229 MS.gene99221:CDS 40.0%
ACCAAGGTTGTTGCAAATGA+TGG - chr8.4:26236697-26236716 None:intergenic 40.0%
AGTCTTGGTTAAGAACACCA+AGG + chr8.4:26237474-26237493 MS.gene99221:CDS 40.0%
CCCCTAGAAGAGGATTATTT+CGG + chr8.4:26237320-26237339 MS.gene99221:CDS 40.0%
CCGAAATAATCCTCTTCTAG+GGG - chr8.4:26237323-26237342 None:intergenic 40.0%
GAAACATCTTCTTGTGGATC+AGG - chr8.4:26237260-26237279 None:intergenic 40.0%
GGAACCAACGTTGAAATGAA+GGG - chr8.4:26236998-26237017 None:intergenic 40.0%
GGGAAAATTACAGTGTTTGC+TGG + chr8.4:26237632-26237651 MS.gene99221:CDS 40.0%
GTTCCACTTCCAAGTTCTAT+GGG + chr8.4:26237744-26237763 MS.gene99221:CDS 40.0%
TAGAACTTGGAAGTGGAACA+TGG - chr8.4:26237743-26237762 None:intergenic 40.0%
TCCATCATTTGCAACAACCT+TGG + chr8.4:26236693-26236712 MS.gene99221:CDS 40.0%
TGCCACAGTGCAAAATACAA+CGG + chr8.4:26236731-26236750 MS.gene99221:CDS 40.0%
TGGAATCGGAAAAGTTGCTT+TGG + chr8.4:26237397-26237416 MS.gene99221:CDS 40.0%
TGGTTTCAAGATGGAGGAAT+TGG - chr8.4:26236518-26236537 None:intergenic 40.0%
TGTTCCACTTCCAAGTTCTA+TGG + chr8.4:26237743-26237762 MS.gene99221:CDS 40.0%
TTTCCCATAGAACTTGGAAG+TGG - chr8.4:26237750-26237769 None:intergenic 40.0%
! AAATAATCCTCTTCTAGGGG+CGG - chr8.4:26237320-26237339 None:intergenic 40.0%
! AGGTTTTTCGCAACGAACAA+CGG - chr8.4:26237240-26237259 None:intergenic 40.0%
! AGTTTTCTCTGTTCCAGCTT+CGG - chr8.4:26237174-26237193 None:intergenic 40.0%
! CAGCTTCATGGTTTTTAACG+CGG - chr8.4:26236818-26236837 None:intergenic 40.0%
! GTTGCAAATGATGGAACATG+TGG - chr8.4:26236688-26236707 None:intergenic 40.0%
! TGGTTTTTAACGCGGTTAAG+TGG - chr8.4:26236810-26236829 None:intergenic 40.0%
! TTGTATTTTGCACTGTGGCA+TGG - chr8.4:26236731-26236750 None:intergenic 40.0%
!! ATGAGATTTTGGAGGCTTTG+GGG + chr8.4:26237696-26237715 MS.gene99221:CDS 40.0%
!! GAGGTTCTAGAGTATGGAAT+CGG + chr8.4:26237383-26237402 MS.gene99221:CDS 40.0%
!! TAGCAATCACTTGTTGTCCA+AGG + chr8.4:26237534-26237553 MS.gene99221:CDS 40.0%
!! TTGGTATTGGAATGGGTTGT+TGG - chr8.4:26236563-26236582 None:intergenic 40.0%
ACTGGTGGAGATCACTTTGA+TGG - chr8.4:26236418-26236437 None:intergenic 45.0%
ACTGTTGTGAGGATGAAAGC+AGG + chr8.4:26237359-26237378 MS.gene99221:CDS 45.0%
AGTGCAAAATACAACGGTCG+TGG + chr8.4:26236737-26236756 MS.gene99221:CDS 45.0%
CATCTTGAAACCATCCAAGC+AGG + chr8.4:26236525-26236544 MS.gene99221:CDS 45.0%
CTACCACTTGGAAACAACCT+TGG - chr8.4:26237494-26237513 None:intergenic 45.0%
CTGCTTGGATGGTTTCAAGA+TGG - chr8.4:26236527-26236546 None:intergenic 45.0%
CTTGGATGGTTTCAAGATGG+AGG - chr8.4:26236524-26236543 None:intergenic 45.0%
GCTAACGAATTACCACTTGG+AGG - chr8.4:26237518-26237537 None:intergenic 45.0%
GGATTATTTCGGAAACGCAG+TGG + chr8.4:26237331-26237350 MS.gene99221:CDS 45.0%
GGGAACCAACGTTGAAATGA+AGG - chr8.4:26236999-26237018 None:intergenic 45.0%
TATTACAAGACGACCAGCGA+GGG - chr8.4:26236640-26236659 None:intergenic 45.0%
TGGTGCTTGAATTGTACTGG+TGG - chr8.4:26236433-26236452 None:intergenic 45.0%
TTAAGTGACGGCGTCTTCAT+TGG + chr8.4:26236873-26236892 MS.gene99221:CDS 45.0%
TTATTACAAGACGACCAGCG+AGG - chr8.4:26236641-26236660 None:intergenic 45.0%
TTATTTCGGAAACGCAGTGG+TGG + chr8.4:26237334-26237353 MS.gene99221:CDS 45.0%
TTGGTTGTGACACAGCTTCA+TGG - chr8.4:26236830-26236849 None:intergenic 45.0%
! AACTCTAGGAGTCCAGGTAA+CGG + chr8.4:26236848-26236867 MS.gene99221:CDS 45.0%
! ACACCAAGGTTGTTTCCAAG+TGG + chr8.4:26237488-26237507 MS.gene99221:CDS 45.0%
! AGCTTTCTTCTAAGGAGCCT+TGG - chr8.4:26237076-26237095 None:intergenic 45.0%
! GACCGTTGTATTTTGCACTG+TGG - chr8.4:26236736-26236755 None:intergenic 45.0%
! TGGGTTGTTGGTGGAAAAGA+AGG - chr8.4:26236551-26236570 None:intergenic 45.0%
!! CAGGTAACGGAGTTAAGTGA+CGG + chr8.4:26236861-26236880 MS.gene99221:CDS 45.0%
!! GTATTGGAATGGGTTGTTGG+TGG - chr8.4:26236560-26236579 None:intergenic 45.0%
ATTACAAGACGACCAGCGAG+GGG - chr8.4:26236639-26236658 None:intergenic 50.0%
CAGTGGTGGTTACTGTTGTG+AGG + chr8.4:26237348-26237367 MS.gene99221:CDS 50.0%
CATGGACGAATAGTGCACCA+AGG - chr8.4:26236713-26236732 None:intergenic 50.0%
CGTCACTTAACTCCGTTACC+TGG - chr8.4:26236863-26236882 None:intergenic 50.0%
GAAAAGAAGGCCTGCTTGGA+TGG - chr8.4:26236538-26236557 None:intergenic 50.0%
GAGGGGTGGAAAGAAGTTGA+GGG - chr8.4:26236622-26236641 None:intergenic 50.0%
GCTGTGTCACAACCAACTCT+AGG + chr8.4:26236834-26236853 MS.gene99221:CDS 50.0%
GGGGAATGACACTGAGTTCA+TGG + chr8.4:26237715-26237734 MS.gene99221:CDS 50.0%
GTTACCTGGACTCCTAGAGT+TGG - chr8.4:26236849-26236868 None:intergenic 50.0%
TCAAAAGCAAACGCCGAAGC+TGG + chr8.4:26237158-26237177 MS.gene99221:CDS 50.0%
TGCAACCACGTTGTTGCAGA+CGG + chr8.4:26236903-26236922 MS.gene99221:CDS 50.0%
TGGAAAGAAGTTGAGGGTGG+AGG - chr8.4:26236616-26236635 None:intergenic 50.0%
TGGAGGAATTGGAGATCCCA+TGG - chr8.4:26236507-26236526 None:intergenic 50.0%
TGTGAGGATGAAAGCAGGAG+AGG + chr8.4:26237364-26237383 MS.gene99221:CDS 50.0%
! ACAACCAACTCTAGGAGTCC+AGG + chr8.4:26236842-26236861 MS.gene99221:CDS 50.0%
! GTGTTTGCTGGTGCTGAAGA+AGG + chr8.4:26237644-26237663 MS.gene99221:CDS 50.0%
! TTGCAGACGGCAAGTCGTTT+TGG + chr8.4:26236916-26236935 MS.gene99221:CDS 50.0%
!! GCAGGAGAGGTTCTAGAGTA+TGG + chr8.4:26237377-26237396 MS.gene99221:CDS 50.0%
!! TTTTGGTTGGGGAAAGCCTG+TGG + chr8.4:26237577-26237596 MS.gene99221:CDS 50.0%
AACTTCTTTCCACCCCTCGC+TGG + chr8.4:26236624-26236643 MS.gene99221:CDS 55.0%
AAGCCTGTGGCAGTTCGAAG+TGG + chr8.4:26237590-26237609 MS.gene99221:CDS 55.0%
ACTTGCCGTCTGCAACAACG+TGG - chr8.4:26236911-26236930 None:intergenic 55.0%
AGCCTGTGGCAGTTCGAAGT+GGG + chr8.4:26237591-26237610 MS.gene99221:CDS 55.0%
ATCCCACTTCGAACTGCCAC+AGG - chr8.4:26237596-26237615 None:intergenic 55.0%
CCAAGTGGTAGTCCTCCAAG+TGG + chr8.4:26237503-26237522 MS.gene99221:CDS 55.0%
CCACTTGGAGGACTACCACT+TGG - chr8.4:26237506-26237525 None:intergenic 55.0%
CGAGGGGTGGAAAGAAGTTG+AGG - chr8.4:26236623-26236642 None:intergenic 55.0%
GGGTGGAAAGAAGTTGAGGG+TGG - chr8.4:26236619-26236638 None:intergenic 55.0%
GGTGGAAAAGAAGGCCTGCT+TGG - chr8.4:26236542-26236561 None:intergenic 55.0%
GTGGCAGTTCGAAGTGGGAT+TGG + chr8.4:26237596-26237615 MS.gene99221:CDS 55.0%
TCTAGGGGCGGAATCAACCT+TGG - chr8.4:26237308-26237327 None:intergenic 55.0%
! GTTGATTCCGCCCCTAGAAG+AGG + chr8.4:26237310-26237329 MS.gene99221:CDS 55.0%
ACAAGACGACCAGCGAGGGG+TGG - chr8.4:26236636-26236655 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 26236408 26237772 26236408 ID=MS.gene99221
chr8.4 mRNA 26236408 26237772 26236408 ID=MS.gene99221.t1;Parent=MS.gene99221
chr8.4 exon 26236408 26237772 26236408 ID=MS.gene99221.t1.exon1;Parent=MS.gene99221.t1
chr8.4 CDS 26236408 26237772 26236408 ID=cds.MS.gene99221.t1;Parent=MS.gene99221.t1
Gene Sequence

>MS.gene99221

ATGGGTTCCATCAAAGTGATCTCCACCAGTACAATTCAAGCACCAATTGACAACAACAATCACAACTCATCAGATTATCAGAAAATTGAATTAACACCATGGGATCTCCAATTCCTCCATCTTGAAACCATCCAAGCAGGCCTTCTTTTCCACCAACAACCCATTCCAATACCAAACCATATAAACCATCTTAAACAAACACTTTCCTCCACCCTCAACTTCTTTCCACCCCTCGCTGGTCGTCTTGTAATAACGCGAGACGATGAACATAACAACGCCACATGTTCCATCATTTGCAACAACCTTGGTGCACTATTCGTCCATGCCACAGTGCAAAATACAACGGTCGTGGATATCCTTCAATCAAACTATACACCTCCAATTGTCCACTCATTTTTTCCACTTAACCGCGTTAAAAACCATGAAGCTGTGTCACAACCAACTCTAGGAGTCCAGGTAACGGAGTTAAGTGACGGCGTCTTCATTGGCTTCACATGCAACCACGTTGTTGCAGACGGCAAGTCGTTTTGGCATTTCGTTAATTCATGGTCACAAATCTCAAAAGGTTCCCACAAAATATCAAAACTCCCTTCATTTCAACGTTGGTTCCCAAACGATATTGAAATTCCAATACACTTCCCTTTCACAAAAACAGAATCACAAAACCAAGGCTCCTTAGAAGAAAGCTTAGAATCCAAGTTAGCCGAAAGAATCTTTCATTTCACAAAGGAGAAAATCGCGGAACTCAAATCAAAAGCAAACGCCGAAGCTGGAACAGAGAAAACTAAAATAACTTCATTTCAAGCGCTATTATCTCACGTTTGGCGTTCCGTTGTTCGTTGCGAAAAACCTGATCCACAAGAAGATGTTTCTTATGTGTTGTTTATAGGTGTTAGACCAAGGTTGATTCCGCCCCTAGAAGAGGATTATTTCGGAAACGCAGTGGTGGTTACTGTTGTGAGGATGAAAGCAGGAGAGGTTCTAGAGTATGGAATCGGAAAAGTTGCTTTGGAGATAAACAAGATGATTTCGTTACATTCTGATAAGATAATAAGGAATCATTACGAGTCTTGGTTAAGAACACCAAGGTTGTTTCCAAGTGGTAGTCCTCCAAGTGGTAATTCGTTAGCAATCACTTGTTGTCCAAGGTTTGATGTTTTTGGCAATGATTTTGGTTGGGGAAAGCCTGTGGCAGTTCGAAGTGGGATTGGTAACAAGTATAATGGGAAAATTACAGTGTTTGCTGGTGCTGAAGAAGGTAGTATAAATGTTGAATTTTGTCTTTCTTATGAGATTTTGGAGGCTTTGGGGAATGACACTGAGTTCATGGATCCATGTTCCACTTCCAAGTTCTATGGGAAATGA
Protein sequence

>MS.gene99221.t1

MGSIKVISTSTIQAPIDNNNHNSSDYQKIELTPWDLQFLHLETIQAGLLFHQQPIPIPNHINHLKQTLSSTLNFFPPLAGRLVITRDDEHNNATCSIICNNLGALFVHATVQNTTVVDILQSNYTPPIVHSFFPLNRVKNHEAVSQPTLGVQVTELSDGVFIGFTCNHVVADGKSFWHFVNSWSQISKGSHKISKLPSFQRWFPNDIEIPIHFPFTKTESQNQGSLEESLESKLAERIFHFTKEKIAELKSKANAEAGTEKTKITSFQALLSHVWRSVVRCEKPDPQEDVSYVLFIGVRPRLIPPLEEDYFGNAVVVTVVRMKAGEVLEYGIGKVALEINKMISLHSDKIIRNHYESWLRTPRLFPSGSPPSGNSLAITCCPRFDVFGNDFGWGKPVAVRSGIGNKYNGKITVFAGAEEGSINVEFCLSYEILEALGNDTEFMDPCSTSKFYGK