AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50379

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50379.t1 MTR_4g022825 93.409 440 29 0 1 440 1 440 0.0 857
MS.gene50379.t1 MTR_4g022800 73.303 442 116 2 1 440 1 442 0.0 682
MS.gene50379.t1 MTR_4g022720 73.923 441 108 2 1 440 1 435 0.0 681
MS.gene50379.t1 MTR_4g022670 73.636 440 115 1 1 439 1 440 0.0 679
MS.gene50379.t1 MTR_7g026860 72.562 441 117 2 1 440 1 438 0.0 669
MS.gene50379.t1 MTR_3g088420 67.500 440 136 1 1 440 1 433 0.0 617
MS.gene50379.t1 MTR_3g026310 64.955 448 149 3 1 440 1 448 0.0 585
MS.gene50379.t1 MTR_4g022760 64.853 441 110 2 1 440 1 397 0.0 579
MS.gene50379.t1 MTR_8g041770 58.889 450 164 6 1 439 1 440 0.0 535
MS.gene50379.t1 MTR_0190s0020 57.206 451 173 7 1 440 1 442 0.0 518
MS.gene50379.t1 MTR_4g094835 54.945 455 173 9 1 436 1 442 1.98e-170 486
MS.gene50379.t1 MTR_4g094842 55.824 455 175 8 1 438 1 446 1.66e-168 481
MS.gene50379.t1 MTR_4g094838 51.535 456 170 10 4 436 1 428 1.25e-153 442
MS.gene50379.t1 MTR_4g094828 50.545 459 197 12 1 439 1 449 4.57e-146 424
MS.gene50379.t1 MTR_4g094832 56.021 382 142 6 69 436 57 426 2.06e-145 421
MS.gene50379.t1 MTR_8g079710 50.873 458 192 12 1 440 1 443 8.64e-144 418
MS.gene50379.t1 MTR_6g016815 52.500 440 136 7 1 440 15 381 4.24e-142 411
MS.gene50379.t1 MTR_4g022290 50.545 459 188 13 1 437 1 442 3.66e-139 406
MS.gene50379.t1 MTR_3g101900 38.462 442 248 10 12 440 18 448 2.07e-92 286
MS.gene50379.t1 MTR_3g102020 38.409 440 243 10 15 437 20 448 7.27e-89 278
MS.gene50379.t1 MTR_8g040760 62.736 212 73 2 1 206 1 212 9.39e-89 269
MS.gene50379.t1 MTR_3g101970 37.004 454 247 11 3 439 5 436 8.43e-87 272
MS.gene50379.t1 MTR_3g101960 37.529 437 246 9 15 435 20 445 1.45e-86 272
MS.gene50379.t1 MTR_3g101930 37.385 436 242 10 24 439 30 454 1.47e-86 271
MS.gene50379.t1 MTR_8g040780 60.185 216 82 2 224 435 12 227 3.01e-85 261
MS.gene50379.t1 MTR_3g102010 37.644 433 243 10 24 440 30 451 3.39e-84 266
MS.gene50379.t1 MTR_7g026850 70.060 167 49 1 29 194 12 178 2.04e-81 249
MS.gene50379.t1 MTR_7g026770 60.000 190 47 2 141 329 132 293 5.84e-71 226
MS.gene50379.t1 MTR_3g070170 32.489 474 262 12 3 436 11 466 3.78e-62 209
MS.gene50379.t1 MTR_5g081960 31.809 481 251 10 16 436 19 482 3.97e-61 206
MS.gene50379.t1 MTR_4g015180 32.577 485 249 11 16 439 19 486 5.71e-60 203
MS.gene50379.t1 MTR_6g045553 30.599 451 277 10 16 436 27 471 1.54e-54 189
MS.gene50379.t1 MTR_4g094825 48.500 200 95 5 5 198 72 269 1.89e-54 186
MS.gene50379.t1 MTR_4g094825 57.823 147 49 2 292 436 252 387 1.00e-48 171
MS.gene50379.t1 MTR_3g102030 34.713 314 164 7 87 396 44 320 3.49e-51 176
MS.gene50379.t1 MTR_6g034495 60.448 134 43 3 57 180 31 164 1.02e-47 161
MS.gene50379.t1 MTR_6g034485 58.333 132 47 1 188 319 2 125 3.05e-46 156
MS.gene50379.t1 MTR_7g026800 50.299 167 53 3 211 376 19 156 1.14e-38 138
MS.gene50379.t1 MTR_4g022780 49.701 167 54 3 211 376 20 157 4.80e-38 136
MS.gene50379.t1 MTR_7g026830 71.429 84 24 0 82 165 14 97 1.21e-36 130
MS.gene50379.t1 MTR_7g026780 56.364 110 24 2 331 440 26 111 6.96e-31 115
MS.gene50379.t1 MTR_8g096530 30.312 353 213 12 49 391 53 382 3.56e-30 122
MS.gene50379.t1 MTR_8g075610 26.531 392 246 14 55 429 47 413 2.52e-29 119
MS.gene50379.t1 MTR_4g007540 30.362 359 214 16 55 398 56 393 1.69e-28 117
MS.gene50379.t1 MTR_2g064495 27.865 384 240 15 55 422 56 418 4.26e-27 112
MS.gene50379.t1 MTR_2g105330 27.604 384 241 15 55 422 56 418 4.43e-27 112
MS.gene50379.t1 MTR_3g098980 27.441 379 237 13 55 421 66 418 8.33e-27 112
MS.gene50379.t1 MTR_3g026950 26.823 384 208 16 52 392 51 404 6.08e-23 101
MS.gene50379.t1 MTR_1g081660 26.303 403 226 15 24 392 31 396 2.03e-22 99.8
MS.gene50379.t1 MTR_3g071320 25.115 434 291 14 17 436 19 432 4.05e-21 95.5
MS.gene50379.t1 MTR_4g013050 26.287 369 187 12 53 392 51 363 6.84e-21 94.7
MS.gene50379.t1 MTR_7g014250 25.056 447 278 16 22 431 24 450 2.21e-20 93.6
MS.gene50379.t1 MTR_2g089650 24.638 414 265 18 38 417 43 443 3.53e-20 92.8
MS.gene50379.t1 MTR_7g026870 74.074 54 14 0 297 350 98 151 9.33e-20 85.9
MS.gene50379.t1 MTR_7g014330 25.176 425 261 16 18 405 20 424 2.88e-19 90.1
MS.gene50379.t1 MTR_3g086020 26.329 395 242 16 55 440 61 415 4.01e-19 89.4
MS.gene50379.t1 MTR_3g112180 25.495 404 244 18 49 427 55 426 1.16e-18 88.2
MS.gene50379.t1 MTR_7g015860 24.272 412 247 14 22 392 26 413 1.31e-18 88.2
MS.gene50379.t1 MTR_7g114520 24.722 360 240 13 44 392 53 392 1.37e-18 88.2
MS.gene50379.t1 MTR_3g049150 24.390 369 229 13 52 391 52 399 1.87e-18 87.8
MS.gene50379.t1 MTR_5g076700 23.832 428 272 16 25 422 24 427 1.90e-18 87.8
MS.gene50379.t1 MTR_7g114550 23.583 441 284 16 25 436 24 440 2.94e-18 87.0
MS.gene50379.t1 MTR_7g026880 68.627 51 16 0 389 439 2 52 3.99e-18 78.2
MS.gene50379.t1 MTR_7g014230 25.806 434 247 15 18 405 22 426 6.25e-18 86.3
MS.gene50379.t1 MTR_6g015320 25.641 312 202 10 137 427 142 444 4.00e-17 83.6
MS.gene50379.t1 MTR_7g114470 23.898 431 269 18 25 422 25 429 4.31e-17 83.6
MS.gene50379.t1 MTR_1g053315 23.810 420 257 18 33 422 60 446 7.32e-17 82.8
MS.gene50379.t1 MTR_7g014160 26.448 397 252 12 55 421 69 455 8.96e-17 82.8
MS.gene50379.t1 MTR_4g097090 23.833 407 274 13 55 439 65 457 8.97e-17 82.4
MS.gene50379.t1 MTR_7g014200 23.025 443 292 12 22 427 28 458 1.09e-16 82.4
MS.gene50379.t1 MTR_2g089670 24.221 417 241 16 21 391 42 429 1.53e-16 82.0
MS.gene50379.t1 MTR_2g089735 26.651 424 251 18 41 424 43 446 1.56e-16 82.0
MS.gene50379.t1 MTR_2g089765 24.757 412 242 14 26 391 45 434 3.74e-16 80.9
MS.gene50379.t1 MTR_7g084940 26.950 282 179 10 53 323 67 332 4.56e-16 80.5
MS.gene50379.t1 MTR_8g012750 23.833 407 265 12 22 391 24 422 1.13e-15 79.3
MS.gene50379.t1 MTR_7g027175 24.611 386 254 14 55 427 68 429 1.23e-15 79.0
MS.gene50379.t1 MTR_6g015830 24.886 438 292 12 17 429 27 452 1.31e-15 79.0
MS.gene50379.t1 MTR_4g055170 27.575 301 199 10 55 350 69 355 1.96e-15 78.6
MS.gene50379.t1 MTR_6g015850 23.611 432 290 12 22 428 32 448 7.54e-15 76.6
MS.gene50379.t1 MTR_7g084910 27.778 270 161 10 53 307 68 318 8.95e-15 76.3
MS.gene50379.t1 MTR_7g085930 26.523 279 186 6 43 308 47 319 1.19e-14 76.3
MS.gene50379.t1 MTR_5g084870 23.148 432 271 15 5 413 4 397 2.35e-14 75.1
MS.gene50379.t1 MTR_7g014610 25.432 405 249 14 55 421 68 457 2.60e-14 75.1
MS.gene50379.t1 MTR_1g040195 24.645 422 260 18 25 427 23 405 3.03e-14 74.7
MS.gene50379.t1 MTR_5g090810 24.362 431 262 19 2 392 6 412 6.76e-14 73.9
MS.gene50379.t1 MTR_1g040215 23.721 430 260 18 8 408 13 403 8.22e-14 73.6
MS.gene50379.t1 MTR_5g090830 24.594 431 261 19 2 392 6 412 3.09e-13 71.6
MS.gene50379.t1 MTR_8g079950 25.337 371 236 14 41 396 42 386 5.15e-13 70.9
MS.gene50379.t1 MTR_7g014360 27.068 266 174 8 138 395 90 343 5.18e-13 70.5
MS.gene50379.t1 MTR_7g086000 26.442 416 254 18 58 439 63 460 1.91e-12 69.3
MS.gene50379.t1 MTR_7g114510 25.681 257 170 9 143 392 8 250 2.08e-12 68.2
MS.gene50379.t1 MTR_7g085990 26.429 280 185 8 43 308 43 315 4.61e-12 68.2
MS.gene50379.t1 MTR_3g450710 27.200 375 214 18 52 392 62 411 5.97e-12 67.8
MS.gene50379.t1 MTR_5g090880 24.728 368 229 16 55 393 67 415 6.12e-12 67.8
MS.gene50379.t1 MTR_3g465990 23.913 276 191 9 55 323 68 331 1.54e-11 66.6
MS.gene50379.t1 MTR_2g015430 23.833 407 243 17 43 428 50 410 1.56e-11 66.2
MS.gene50379.t1 MTR_7g013850 21.831 426 263 16 52 427 65 470 3.09e-11 65.5
MS.gene50379.t1 MTR_3g450690 27.151 372 215 18 52 392 69 415 5.89e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50379.t1 AT5G67150 43.119 436 225 11 16 439 21 445 3.70e-112 337
MS.gene50379.t1 AT3G50300 41.839 435 228 11 1 421 1 424 5.48e-99 303
MS.gene50379.t1 AT3G50280 40.371 431 239 8 16 437 19 440 2.26e-98 302
MS.gene50379.t1 AT5G67160 38.426 432 237 10 17 440 22 432 2.32e-92 286
MS.gene50379.t1 AT3G50270 37.748 453 260 10 2 440 4 448 5.05e-91 283
MS.gene50379.t1 AT5G42830 37.355 431 245 10 24 440 30 449 5.49e-85 268
MS.gene50379.t1 AT5G38130 39.141 419 227 12 16 421 39 442 7.76e-84 265
MS.gene50379.t1 AT5G07860 37.701 435 235 10 24 439 31 448 1.22e-80 256
MS.gene50379.t1 AT5G07850 37.071 437 241 12 24 440 31 453 1.26e-75 243
MS.gene50379.t1 AT5G07870 38.061 423 236 10 38 440 45 461 5.66e-74 239
MS.gene50379.t1 AT2G39980 33.264 478 246 13 16 439 21 479 9.41e-68 224
MS.gene50379.t1 AT3G50280 38.871 319 180 5 125 437 1 310 1.96e-66 215
MS.gene50379.t1 AT5G01210 34.328 469 246 13 16 440 19 469 6.19e-64 213
MS.gene50379.t1 AT5G23940 32.533 458 270 13 5 436 4 448 6.39e-64 214
MS.gene50379.t1 AT3G50290 40.876 274 148 7 122 389 4 269 9.71e-51 173
MS.gene50379.t1 AT5G48930 27.717 368 216 9 53 398 54 393 1.01e-28 117
MS.gene50379.t1 AT3G29670 25.352 426 277 14 20 421 25 433 1.67e-25 108
MS.gene50379.t1 AT3G48720 27.473 364 229 12 42 392 44 385 1.22e-24 105
MS.gene50379.t1 AT3G29590 26.503 449 297 17 3 431 7 442 3.53e-24 104
MS.gene50379.t1 AT2G19070 25.054 459 271 21 27 438 19 451 9.53e-23 100
MS.gene50379.t1 AT5G41040 26.685 356 224 14 50 392 64 395 1.95e-21 96.7
MS.gene50379.t1 AT5G41040 26.685 356 224 14 50 392 80 411 2.35e-21 96.7
MS.gene50379.t1 AT5G39080 26.485 404 243 18 52 421 62 445 1.85e-20 94.0
MS.gene50379.t1 AT5G57840 27.873 409 242 18 41 421 39 422 4.56e-20 92.4
MS.gene50379.t1 AT3G29635 25.000 436 267 16 22 421 27 438 1.71e-19 90.9
MS.gene50379.t1 AT3G29680 26.131 398 247 15 49 421 56 431 2.56e-19 90.5
MS.gene50379.t1 AT5G63560 26.478 389 239 16 50 422 56 413 5.65e-19 89.0
MS.gene50379.t1 AT5G39050 24.543 383 225 13 66 414 90 442 1.83e-18 87.8
MS.gene50379.t1 AT2G40230 24.645 422 287 16 22 431 24 426 4.71e-18 86.3
MS.gene50379.t1 AT1G24430 24.654 434 262 19 26 440 38 425 6.87e-18 85.9
MS.gene50379.t1 AT3G26040 24.455 413 255 18 49 440 53 429 9.99e-18 85.5
MS.gene50379.t1 AT1G28680 25.377 398 247 15 58 440 65 427 4.16e-17 83.6
MS.gene50379.t1 AT5G39090 27.007 274 169 12 135 391 140 399 7.01e-16 79.7
MS.gene50379.t1 AT1G03390 25.490 408 255 19 42 422 59 444 1.85e-15 78.6
MS.gene50379.t1 AT5G07080 26.102 295 187 12 42 321 53 331 2.64e-15 78.2
MS.gene50379.t1 AT5G61160 21.805 399 266 14 22 392 23 403 5.46e-15 77.0
MS.gene50379.t1 AT1G28680 25.253 297 201 9 58 348 65 346 1.25e-14 75.9
MS.gene50379.t1 AT3G30280 24.000 375 211 17 54 398 61 391 9.47e-14 73.2
MS.gene50379.t1 AT5G17540 25.000 440 275 20 25 434 23 437 1.18e-13 73.2
MS.gene50379.t1 AT1G78990 26.344 372 229 17 38 392 53 396 1.41e-13 72.8
MS.gene50379.t1 AT5G23970 26.222 450 280 21 4 436 3 417 5.70e-13 70.9
MS.gene50379.t1 AT2G23510 24.611 386 255 18 55 422 69 436 7.21e-13 70.5
MS.gene50379.t1 AT1G65450 25.352 284 188 12 53 324 70 341 9.06e-13 70.5
MS.gene50379.t1 AT5G16410 22.970 431 283 17 1 413 45 444 1.43e-12 69.7
MS.gene50379.t1 AT1G32910 25.876 371 210 18 53 392 63 399 1.97e-12 69.3
MS.gene50379.t1 AT1G03940 23.192 401 280 11 50 428 61 455 2.73e-12 68.9
MS.gene50379.t1 AT2G25150 25.414 362 234 14 50 395 62 403 6.29e-12 67.8
MS.gene50379.t1 AT4G15390 23.704 405 252 17 10 394 24 391 9.10e-12 67.0
MS.gene50379.t1 AT4G15390 23.704 405 252 17 10 394 24 391 9.10e-12 67.0
MS.gene50379.t1 AT3G47170 25.503 298 185 11 44 321 64 344 9.37e-12 67.0

Find 82 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCATGGTTTGATGTTTA+TGG 0.113342 4.4:-78886396 MS.gene50379:CDS
GTGTCTTCCTTACAAGATTT+TGG 0.202537 4.4:-78886265 MS.gene50379:CDS
CCATTGCCAGAAGATTATTT+TGG 0.248348 4.4:-78886627 MS.gene50379:CDS
GATGTTTATGGTAATGATTT+TGG 0.308658 4.4:-78886384 MS.gene50379:CDS
TGGTTGGATAAAGTAGAAAT+TGG 0.327409 4.4:+78887435 None:intergenic
GTGCTTAATACGTTCATAAT+AGG 0.328958 4.4:-78886672 MS.gene50379:CDS
TGATCATGAATTCATGGATA+TGG 0.332225 4.4:-78886229 MS.gene50379:CDS
AATGTCTTAGCCGTTGAATT+TGG 0.349561 4.4:+78887372 None:intergenic
TTCTTGTGGATCAAGATGTT+TGG 0.349905 4.4:+78886698 None:intergenic
AATGAAAGCTGGGGAGTTGT+TGG 0.360707 4.4:-78886577 MS.gene50379:CDS
GAAACCAACGCTCTAGTGTT+GGG 0.360883 4.4:+78886961 None:intergenic
TTTCATTGCTTTGATTGTAT+TGG 0.364692 4.4:+78887496 None:intergenic
GGAATGGTTTGTGCCTTTGC+TGG 0.368156 4.4:-78886318 MS.gene50379:CDS
TGAGAAATCCAAGCTTCATT+AGG 0.373854 4.4:-78886461 MS.gene50379:CDS
GCGTTTCCAAAATAATCTTC+TGG 0.379778 4.4:+78886621 None:intergenic
GCCAGCAAGAGGTTCGAAAA+AGG 0.381613 4.4:+78887331 None:intergenic
CTAGCTGACGGCATCTTCAT+AGG 0.398680 4.4:-78887086 MS.gene50379:CDS
GGGAGTTGTTGGAGGAAGGT+GGG 0.400037 4.4:-78886566 MS.gene50379:CDS
GAAACGCGATTATAGGTTGC+GGG 0.410719 4.4:-78886605 MS.gene50379:CDS
ATGGGCTGTTGAATACCATT+TGG 0.419783 4.4:+78886939 None:intergenic
TTGTATTGGTGGAGAGTACT+TGG 0.455345 4.4:+78887510 None:intergenic
AAACCATGGAGAACTGCTAA+TGG 0.455624 4.4:+78886407 None:intergenic
GGGAAATGATCATGAATTCA+TGG 0.457595 4.4:-78886235 MS.gene50379:CDS
TTGTTGGAGGAAGGTGGGAT+TGG 0.457605 4.4:-78886561 MS.gene50379:CDS
GGGGAGTTGTTGGAGGAAGG+TGG 0.459892 4.4:-78886567 MS.gene50379:CDS
CGGAGTGGAAATAGAACAAA+TGG 0.464324 4.4:-78886339 MS.gene50379:CDS
TGCGGGTTTAAAATGAAAGC+TGG 0.465815 4.4:-78886588 MS.gene50379:CDS
GGCAATGGTGGAACAAGTCT+TGG 0.472119 4.4:+78886642 None:intergenic
CCAAAATAATCTTCTGGCAA+TGG 0.477773 4.4:+78886627 None:intergenic
GCTGGGGAGTTGTTGGAGGA+AGG 0.483907 4.4:-78886570 MS.gene50379:CDS
AAAACCCACATATGTTCCTT+CGG 0.485243 4.4:-78887192 MS.gene50379:CDS
GAGGAAGGTGGGATTGGTAA+AGG 0.486075 4.4:-78886555 MS.gene50379:CDS
CATTGCTTTGATTGTATTGG+TGG 0.486507 4.4:+78887499 None:intergenic
CTATCAAGCCTAATGAAGCT+TGG 0.491157 4.4:+78886453 None:intergenic
GGGGAAAGCCTGTGGCAGTT+CGG 0.491671 4.4:-78886359 MS.gene50379:CDS
ATAAAATTGATTTAACTCCA+TGG 0.501405 4.4:-78887463 MS.gene50379:CDS
ATTGCCTCCAAAATCTTGTA+AGG 0.504306 4.4:+78886258 None:intergenic
TAAAATTGATTTAACTCCAT+GGG 0.505681 4.4:-78887462 MS.gene50379:CDS
GGAAACCAACGCTCTAGTGT+TGG 0.511281 4.4:+78886960 None:intergenic
GCCTTTGCTGGAATAGAAGA+AGG 0.511653 4.4:-78886306 MS.gene50379:CDS
TTCAATCATGTTGTAGCTGA+CGG 0.511810 4.4:-78887056 MS.gene50379:CDS
TATAGTTAAAGGAAATCGTA+TGG 0.515144 4.4:+78886920 None:intergenic
CTAGAGCGTTGGTTTCCAAA+TGG 0.515960 4.4:-78886954 MS.gene50379:CDS
TTTCCACTCCGAACTGCCAC+AGG 0.522499 4.4:+78886351 None:intergenic
TGAATTTGGTCTGATGCTAC+AGG 0.526430 4.4:+78887386 None:intergenic
TGTAAGGAAGACACACTTGA+AGG 0.538009 4.4:+78886274 None:intergenic
TAAAGAATGATTATGAGTCA+TGG 0.538860 4.4:-78886485 MS.gene50379:CDS
GCGGGTTTAAAATGAAAGCT+GGG 0.538973 4.4:-78886587 MS.gene50379:CDS
AGGTACATCAAAATCATTGT+TGG 0.540953 4.4:-78887126 MS.gene50379:CDS
GAAAGCTGGGGAGTTGTTGG+AGG 0.541663 4.4:-78886574 MS.gene50379:CDS
TGGCCATTAGCAGTTCTCCA+TGG 0.541677 4.4:-78886410 MS.gene50379:CDS
AAATAATCTTCTGGCAATGG+TGG 0.542517 4.4:+78886630 None:intergenic
GGAAACGCGATTATAGGTTG+CGG 0.544739 4.4:-78886606 MS.gene50379:CDS
TAGTTTGTAACTCCGTTGAA+AGG 0.548129 4.4:+78887152 None:intergenic
CGTGTTCTGTAACTTTGAGA+CGG 0.548497 4.4:+78887309 None:intergenic
TGGAAATAGAACAAATGGAA+TGG 0.553380 4.4:-78886334 MS.gene50379:CDS
GTATTAAGCACATCTTCTTG+TGG 0.555043 4.4:+78886684 None:intergenic
ACCTTCTTCTATTCCAGCAA+AGG 0.555908 4.4:+78886305 None:intergenic
AGTAGAAATTGGAGATCCCA+TGG 0.565431 4.4:+78887446 None:intergenic
AAACCAACGCTCTAGTGTTG+GGG 0.569521 4.4:+78886962 None:intergenic
ATGGCATTTCATCAATTCGT+GGG 0.570140 4.4:-78887024 MS.gene50379:CDS
AACAACCGAAGGAACATATG+TGG 0.575430 4.4:+78887187 None:intergenic
ATAGTTAAAGGAAATCGTAT+GGG 0.585010 4.4:+78886921 None:intergenic
CTACAGGATGGTGATAAAGA+AGG 0.592406 4.4:+78887402 None:intergenic
CATGGCATTTCATCAATTCG+TGG 0.610417 4.4:-78887025 MS.gene50379:CDS
ACTTTGAGACGGCCAGCAAG+AGG 0.616523 4.4:+78887320 None:intergenic
TCTGTAACATGCAATAATGC+AGG 0.619827 4.4:-78887266 MS.gene50379:CDS
ATTGTTGGCAGTTCAAGTGA+CGG 0.619862 4.4:-78887111 MS.gene50379:CDS
AACCAACGCTCTAGTGTTGG+GGG 0.632370 4.4:+78886963 None:intergenic
TAGCTGACGGACAGTCCACA+TGG 0.634434 4.4:-78887043 MS.gene50379:CDS
CAAGTGACGGAGCTAGCTGA+CGG 0.639821 4.4:-78887098 MS.gene50379:CDS
CGGGTTTAAAATGAAAGCTG+GGG 0.642028 4.4:-78886586 MS.gene50379:CDS
AAGCCTGTGGCAGTTCGGAG+TGG 0.647498 4.4:-78886354 MS.gene50379:CDS
ACAACCGAAGGAACATATGT+GGG 0.650889 4.4:+78887188 None:intergenic
AACGGAGTTACAAACTACGA+AGG 0.656439 4.4:-78887146 MS.gene50379:CDS
TTACCATAAACATCAAACCA+TGG 0.659822 4.4:+78886393 None:intergenic
TAGCATCAGACCAAATTCAA+CGG 0.663707 4.4:-78887382 MS.gene50379:CDS
AGGTGTAAACTTTATACATG+CGG 0.673861 4.4:-78887246 MS.gene50379:CDS
GCCACAAAAGAATTACTCTG+AGG 0.711684 4.4:-78886895 MS.gene50379:CDS
CGAATTGATGAAATGCCATG+TGG 0.718082 4.4:+78887028 None:intergenic
TTTGGTCTGATGCTACAGGA+TGG 0.756053 4.4:+78887390 None:intergenic
TCGTGGGCTGAAATGTCACG+TGG 0.758657 4.4:-78887008 MS.gene50379:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAAATTGATTTAACTCCA+TGG - chr4.4:78886279-78886298 MS.gene50379:CDS 20.0%
!!! TAAAATTGATTTAACTCCAT+GGG - chr4.4:78886280-78886299 MS.gene50379:CDS 20.0%
!!! TTGTAGAGAAGATATTTTTT+TGG + chr4.4:78886987-78887006 None:intergenic 20.0%
! AAAAATTCTATCTATCTGAG+AGG - chr4.4:78886897-78886916 MS.gene50379:CDS 25.0%
! ATAGTTAAAGGAAATCGTAT+GGG + chr4.4:78886824-78886843 None:intergenic 25.0%
! TAAAGAATGATTATGAGTCA+TGG - chr4.4:78887257-78887276 MS.gene50379:CDS 25.0%
! TATAGTTAAAGGAAATCGTA+TGG + chr4.4:78886825-78886844 None:intergenic 25.0%
! TTTCATTGCTTTGATTGTAT+TGG + chr4.4:78886249-78886268 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG - chr4.4:78887358-78887377 MS.gene50379:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG - chr4.4:78887363-78887382 MS.gene50379:CDS 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG - chr4.4:78887362-78887381 MS.gene50379:CDS 25.0%
AGGTACATCAAAATCATTGT+TGG - chr4.4:78886616-78886635 MS.gene50379:CDS 30.0%
AGGTGTAAACTTTATACATG+CGG - chr4.4:78886496-78886515 MS.gene50379:CDS 30.0%
CTTTATCCAACCAACAAAAA+AGG - chr4.4:78886317-78886336 MS.gene50379:CDS 30.0%
GTGCTTAATACGTTCATAAT+AGG - chr4.4:78887070-78887089 MS.gene50379:CDS 30.0%
TGATCATGAATTCATGGATA+TGG - chr4.4:78887513-78887532 MS.gene50379:CDS 30.0%
TGGAAATAGAACAAATGGAA+TGG - chr4.4:78887408-78887427 MS.gene50379:CDS 30.0%
TGGTTGGATAAAGTAGAAAT+TGG + chr4.4:78886310-78886329 None:intergenic 30.0%
TTACCATAAACATCAAACCA+TGG + chr4.4:78887352-78887371 None:intergenic 30.0%
!! TATGGTAATGATTTTGGATG+GGG - chr4.4:78887364-78887383 MS.gene50379:CDS 30.0%
!! TATTTTGGAAACGCGATTAT+AGG - chr4.4:78887130-78887149 MS.gene50379:CDS 30.0%
!! TTTTGTGGCTCTATAGTTAA+AGG + chr4.4:78886836-78886855 None:intergenic 30.0%
!!! ATAAAGAAGGCCTTTTTTGT+TGG + chr4.4:78886330-78886349 None:intergenic 30.0%
!!! CGTTCATTTTTTCCTTTCAA+CGG - chr4.4:78886578-78886597 MS.gene50379:CDS 30.0%
!!! CTTGAGCACTAAGAATTTTT+TGG - chr4.4:78887312-78887331 MS.gene50379:CDS 30.0%
AAAAATGAACGAACAACCGA+AGG + chr4.4:78886569-78886588 None:intergenic 35.0%
AAAACCCACATATGTTCCTT+CGG - chr4.4:78886550-78886569 MS.gene50379:CDS 35.0%
AAATAATCTTCTGGCAATGG+TGG + chr4.4:78887115-78887134 None:intergenic 35.0%
AAGAGGTTCGAAAAAGGAAA+GGG + chr4.4:78886408-78886427 None:intergenic 35.0%
AATGTCTTAGCCGTTGAATT+TGG + chr4.4:78886373-78886392 None:intergenic 35.0%
ATGGCATTTCATCAATTCGT+GGG - chr4.4:78886718-78886737 MS.gene50379:CDS 35.0%
ATTGCCTCCAAAATCTTGTA+AGG + chr4.4:78887487-78887506 None:intergenic 35.0%
CATTGCTTTGATTGTATTGG+TGG + chr4.4:78886246-78886265 None:intergenic 35.0%
CCAAAATAATCTTCTGGCAA+TGG + chr4.4:78887118-78887137 None:intergenic 35.0%
GCGTTTCCAAAATAATCTTC+TGG + chr4.4:78887124-78887143 None:intergenic 35.0%
GGGAAATGATCATGAATTCA+TGG - chr4.4:78887507-78887526 MS.gene50379:CDS 35.0%
GTATTAAGCACATCTTCTTG+TGG + chr4.4:78887061-78887080 None:intergenic 35.0%
TAGCATCAGACCAAATTCAA+CGG - chr4.4:78886360-78886379 MS.gene50379:CDS 35.0%
TCTCCATGGTTTGATGTTTA+TGG - chr4.4:78887346-78887365 MS.gene50379:CDS 35.0%
TCTGTAACATGCAATAATGC+AGG - chr4.4:78886476-78886495 MS.gene50379:CDS 35.0%
TGAGAAATCCAAGCTTCATT+AGG - chr4.4:78887281-78887300 MS.gene50379:CDS 35.0%
TTCAATCATGTTGTAGCTGA+CGG - chr4.4:78886686-78886705 MS.gene50379:CDS 35.0%
TTCTTGTGGATCAAGATGTT+TGG + chr4.4:78887047-78887066 None:intergenic 35.0%
! AAGGAACATATGTGGGTTTT+AGG + chr4.4:78886550-78886569 None:intergenic 35.0%
! CCATTGCCAGAAGATTATTT+TGG - chr4.4:78887115-78887134 MS.gene50379:CDS 35.0%
! GTGTCTTCCTTACAAGATTT+TGG - chr4.4:78887477-78887496 MS.gene50379:CDS 35.0%
! TAGTTTGTAACTCCGTTGAA+AGG + chr4.4:78886593-78886612 None:intergenic 35.0%
! TCCTCAGAGTAATTCTTTTG+TGG + chr4.4:78886851-78886870 None:intergenic 35.0%
! TCTTCCTTACAAGATTTTGG+AGG - chr4.4:78887480-78887499 MS.gene50379:CDS 35.0%
!! AAGCGCTTTTAACTCATCTT+TGG - chr4.4:78887005-78887024 MS.gene50379:CDS 35.0%
!! TACAAGATTTTGGAGGCAAT+GGG - chr4.4:78887487-78887506 MS.gene50379:CDS 35.0%
!! TTACAAGATTTTGGAGGCAA+TGG - chr4.4:78887486-78887505 MS.gene50379:CDS 35.0%
AAACCATGGAGAACTGCTAA+TGG + chr4.4:78887338-78887357 None:intergenic 40.0%
AACAACCGAAGGAACATATG+TGG + chr4.4:78886558-78886577 None:intergenic 40.0%
AACGGAGTTACAAACTACGA+AGG - chr4.4:78886596-78886615 MS.gene50379:CDS 40.0%
ACAACCGAAGGAACATATGT+GGG + chr4.4:78886557-78886576 None:intergenic 40.0%
ACCTTCTTCTATTCCAGCAA+AGG + chr4.4:78887440-78887459 None:intergenic 40.0%
AGTAGAAATTGGAGATCCCA+TGG + chr4.4:78886299-78886318 None:intergenic 40.0%
ATTGTTGGCAGTTCAAGTGA+CGG - chr4.4:78886631-78886650 MS.gene50379:CDS 40.0%
CAAGAGGTTCGAAAAAGGAA+AGG + chr4.4:78886409-78886428 None:intergenic 40.0%
CATGGCATTTCATCAATTCG+TGG - chr4.4:78886717-78886736 MS.gene50379:CDS 40.0%
CGAATTGATGAAATGCCATG+TGG + chr4.4:78886717-78886736 None:intergenic 40.0%
CGGAGTGGAAATAGAACAAA+TGG - chr4.4:78887403-78887422 MS.gene50379:CDS 40.0%
CTACAGGATGGTGATAAAGA+AGG + chr4.4:78886343-78886362 None:intergenic 40.0%
CTATCAAGCCTAATGAAGCT+TGG + chr4.4:78887292-78887311 None:intergenic 40.0%
GCCACAAAAGAATTACTCTG+AGG - chr4.4:78886847-78886866 MS.gene50379:CDS 40.0%
TGAATTTGGTCTGATGCTAC+AGG + chr4.4:78886359-78886378 None:intergenic 40.0%
TGTAAGGAAGACACACTTGA+AGG + chr4.4:78887471-78887490 None:intergenic 40.0%
! ATGGGCTGTTGAATACCATT+TGG + chr4.4:78886806-78886825 None:intergenic 40.0%
! CGGGTTTAAAATGAAAGCTG+GGG - chr4.4:78887156-78887175 MS.gene50379:CDS 40.0%
! CGTGTTCTGTAACTTTGAGA+CGG + chr4.4:78886436-78886455 None:intergenic 40.0%
! GCGGGTTTAAAATGAAAGCT+GGG - chr4.4:78887155-78887174 MS.gene50379:CDS 40.0%
! TGCGGGTTTAAAATGAAAGC+TGG - chr4.4:78887154-78887173 MS.gene50379:CDS 40.0%
!! TTGTATTGGTGGAGAGTACT+TGG + chr4.4:78886235-78886254 None:intergenic 40.0%
!!! AGAAGGCCTTTTTTGTTGGT+TGG + chr4.4:78886326-78886345 None:intergenic 40.0%
AAACCAACGCTCTAGTGTTG+GGG + chr4.4:78886783-78886802 None:intergenic 45.0%
AATGAAAGCTGGGGAGTTGT+TGG - chr4.4:78887165-78887184 MS.gene50379:CDS 45.0%
GAAACCAACGCTCTAGTGTT+GGG + chr4.4:78886784-78886803 None:intergenic 45.0%
GAAACGCGATTATAGGTTGC+GGG - chr4.4:78887137-78887156 MS.gene50379:CDS 45.0%
GCCTTTGCTGGAATAGAAGA+AGG - chr4.4:78887436-78887455 MS.gene50379:CDS 45.0%
GGAAACGCGATTATAGGTTG+CGG - chr4.4:78887136-78887155 MS.gene50379:CDS 45.0%
TTTGGTCTGATGCTACAGGA+TGG + chr4.4:78886355-78886374 None:intergenic 45.0%
!! CTAGAGCGTTGGTTTCCAAA+TGG - chr4.4:78886788-78886807 MS.gene50379:CDS 45.0%
!!! TCCTTTTTCGAACCTCTTGC+TGG - chr4.4:78886410-78886429 MS.gene50379:CDS 45.0%
CTAGCTGACGGCATCTTCAT+AGG - chr4.4:78886656-78886675 MS.gene50379:CDS 50.0%
GCCAGCAAGAGGTTCGAAAA+AGG + chr4.4:78886414-78886433 None:intergenic 50.0%
GGAAACCAACGCTCTAGTGT+TGG + chr4.4:78886785-78886804 None:intergenic 50.0%
TGGCCATTAGCAGTTCTCCA+TGG - chr4.4:78887332-78887351 MS.gene50379:CDS 50.0%
TTGTTGGAGGAAGGTGGGAT+TGG - chr4.4:78887181-78887200 MS.gene50379:CDS 50.0%
! AACCAACGCTCTAGTGTTGG+GGG + chr4.4:78886782-78886801 None:intergenic 50.0%
! GAGGAAGGTGGGATTGGTAA+AGG - chr4.4:78887187-78887206 MS.gene50379:CDS 50.0%
! GGAATGGTTTGTGCCTTTGC+TGG - chr4.4:78887424-78887443 MS.gene50379:CDS 50.0%
! GGCAATGGTGGAACAAGTCT+TGG + chr4.4:78887103-78887122 None:intergenic 50.0%
! TTTTGGATGGGGAAAGCCTG+TGG - chr4.4:78887375-78887394 MS.gene50379:CDS 50.0%
AACCCCCAACACTAGAGCGT+TGG - chr4.4:78886777-78886796 MS.gene50379:CDS 55.0%
GAAAGCTGGGGAGTTGTTGG+AGG - chr4.4:78887168-78887187 MS.gene50379:CDS 55.0%
GGGAGTTGTTGGAGGAAGGT+GGG - chr4.4:78887176-78887195 MS.gene50379:CDS 55.0%
TTTCCACTCCGAACTGCCAC+AGG + chr4.4:78887394-78887413 None:intergenic 55.0%
! CAAGTGACGGAGCTAGCTGA+CGG - chr4.4:78886644-78886663 MS.gene50379:CDS 55.0%
! TAGCTGACGGACAGTCCACA+TGG - chr4.4:78886699-78886718 MS.gene50379:CDS 55.0%
! TCGTGGGCTGAAATGTCACG+TGG - chr4.4:78886734-78886753 MS.gene50379:CDS 55.0%
!! ACTTTGAGACGGCCAGCAAG+AGG + chr4.4:78886425-78886444 None:intergenic 55.0%
AAGCCTGTGGCAGTTCGGAG+TGG - chr4.4:78887388-78887407 MS.gene50379:CDS 60.0%
GCTGGGGAGTTGTTGGAGGA+AGG - chr4.4:78887172-78887191 MS.gene50379:CDS 60.0%
GGGGAAAGCCTGTGGCAGTT+CGG - chr4.4:78887383-78887402 MS.gene50379:CDS 60.0%
GGGGAGTTGTTGGAGGAAGG+TGG - chr4.4:78887175-78887194 MS.gene50379:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 78886221 78887543 78886221 ID=MS.gene50379
chr4.4 mRNA 78886221 78887543 78886221 ID=MS.gene50379.t1;Parent=MS.gene50379
chr4.4 exon 78886221 78887543 78886221 ID=MS.gene50379.t1.exon1;Parent=MS.gene50379.t1
chr4.4 CDS 78886221 78887543 78886221 ID=cds.MS.gene50379.t1;Parent=MS.gene50379.t1
Gene Sequence

>MS.gene50379

ATGGGTGCATTCCAAGTACTCTCCACCAATACAATCAAAGCAATGAAATCAAGTAATCATAAAATTGATTTAACTCCATGGGATCTCCAATTTCTACTTTATCCAACCAACAAAAAAGGCCTTCTTTATCACCATCCTGTAGCATCAGACCAAATTCAACGGCTAAGACATTCTCTTTCTTCTGCCCTTTCCTTTTTCGAACCTCTTGCTGGCCGTCTCAAAGTTACAGAACACGAAGACAGTACTGTCTCGTGTTCTGTAACATGCAATAATGCAGGTGTAAACTTTATACATGCGGCAACAAAGAATACTTGTGTTGCTGACATCCTAAAACCCACATATGTTCCTTCGGTTGTTCGTTCATTTTTTCCTTTCAACGGAGTTACAAACTACGAAGGTACATCAAAATCATTGTTGGCAGTTCAAGTGACGGAGCTAGCTGACGGCATCTTCATAGGCTGTAATTTCAATCATGTTGTAGCTGACGGACAGTCCACATGGCATTTCATCAATTCGTGGGCTGAAATGTCACGTGGTTGTCATCATCAAATATCTAAACCCCCAACACTAGAGCGTTGGTTTCCAAATGGTATTCAACAGCCCATACGATTTCCTTTAACTATAGAGCCACAAAAGAATTACTCTGAGGAGTTGAGTTTTTCGTCATCTGATGACGAAAAATTCTATCTATCTGAGAGGATATTTCACTTCACAAAGAAGAAAATCATGCAATTGAAATCAAAAGTCAACGCAGCGATTGATACCAAAAAAATATCTTCTCTACAAGCGCTTTTAACTCATCTTTGGTGTTCAGTGATTCGTTCCAAACATCTTGATCCACAAGAAGATGTGCTTAATACGTTCATAATAGGAGTTAGACCAAGACTTGTTCCACCATTGCCAGAAGATTATTTTGGAAACGCGATTATAGGTTGCGGGTTTAAAATGAAAGCTGGGGAGTTGTTGGAGGAAGGTGGGATTGGTAAAGGTGCTTTGTTGATGAATAAGTTAATTGCTTTGCACTCTAATGAGAAATTAAAGAATGATTATGAGTCATGGTTGAGAAATCCAAGCTTCATTAGGCTTGATAGCTTGAGCACTAAGAATTTTTTGGCCATTAGCAGTTCTCCATGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAGCCTGTGGCAGTTCGGAGTGGAAATAGAACAAATGGAATGGTTTGTGCCTTTGCTGGAATAGAAGAAGGTAGCATTGACCTTCAAGTGTGTCTTCCTTACAAGATTTTGGAGGCAATGGGAAATGATCATGAATTCATGGATATGGTTAACTAA
Protein sequence

>MS.gene50379.t1

MGAFQVLSTNTIKAMKSSNHKIDLTPWDLQFLLYPTNKKGLLYHHPVASDQIQRLRHSLSSALSFFEPLAGRLKVTEHEDSTVSCSVTCNNAGVNFIHAATKNTCVADILKPTYVPSVVRSFFPFNGVTNYEGTSKSLLAVQVTELADGIFIGCNFNHVVADGQSTWHFINSWAEMSRGCHHQISKPPTLERWFPNGIQQPIRFPLTIEPQKNYSEELSFSSSDDEKFYLSERIFHFTKKKIMQLKSKVNAAIDTKKISSLQALLTHLWCSVIRSKHLDPQEDVLNTFIIGVRPRLVPPLPEDYFGNAIIGCGFKMKAGELLEEGGIGKGALLMNKLIALHSNEKLKNDYESWLRNPSFIRLDSLSTKNFLAISSSPWFDVYGNDFGWGKPVAVRSGNRTNGMVCAFAGIEEGSIDLQVCLPYKILEAMGNDHEFMDMVN