AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87537

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87537.t1 MTR_4g022825 93.636 440 28 0 1 440 1 440 0.0 860
MS.gene87537.t1 MTR_4g022800 73.077 442 117 2 1 440 1 442 0.0 682
MS.gene87537.t1 MTR_4g022720 73.696 441 109 2 1 440 1 435 0.0 681
MS.gene87537.t1 MTR_4g022670 73.409 440 116 1 1 439 1 440 0.0 678
MS.gene87537.t1 MTR_7g026860 72.109 441 119 2 1 440 1 438 0.0 667
MS.gene87537.t1 MTR_3g088420 67.273 440 137 1 1 440 1 433 0.0 616
MS.gene87537.t1 MTR_3g026310 64.732 448 150 3 1 440 1 448 0.0 585
MS.gene87537.t1 MTR_4g022760 64.626 441 111 2 1 440 1 397 0.0 578
MS.gene87537.t1 MTR_8g041770 58.667 450 165 6 1 439 1 440 0.0 534
MS.gene87537.t1 MTR_0190s0020 56.984 451 174 7 1 440 1 442 0.0 518
MS.gene87537.t1 MTR_4g094835 55.385 455 171 9 1 436 1 442 2.18e-171 488
MS.gene87537.t1 MTR_4g094842 56.264 455 173 8 1 438 1 446 3.06e-169 483
MS.gene87537.t1 MTR_4g094838 51.974 456 168 10 4 436 1 428 1.09e-154 445
MS.gene87537.t1 MTR_4g094828 50.980 459 195 12 1 439 1 449 1.40e-147 428
MS.gene87537.t1 MTR_4g094832 56.283 382 141 6 69 436 57 426 7.75e-146 422
MS.gene87537.t1 MTR_8g079710 51.310 458 190 12 1 440 1 443 6.00e-145 421
MS.gene87537.t1 MTR_6g016815 52.273 440 137 7 1 440 15 381 2.81e-142 412
MS.gene87537.t1 MTR_4g022290 50.980 459 186 13 1 437 1 442 9.15e-141 410
MS.gene87537.t1 MTR_3g101900 38.739 444 244 12 12 440 18 448 8.29e-92 285
MS.gene87537.t1 MTR_8g040760 62.736 212 73 2 1 206 1 212 3.70e-89 270
MS.gene87537.t1 MTR_3g102020 38.182 440 244 10 15 437 20 448 3.64e-88 276
MS.gene87537.t1 MTR_3g101930 37.385 436 242 10 24 439 30 454 2.41e-86 271
MS.gene87537.t1 MTR_3g101970 36.784 454 248 11 3 439 5 436 3.06e-86 270
MS.gene87537.t1 MTR_3g101960 37.300 437 247 9 15 435 20 445 6.52e-86 270
MS.gene87537.t1 MTR_8g040780 59.722 216 83 2 224 435 12 227 1.11e-84 260
MS.gene87537.t1 MTR_3g102010 37.413 433 244 10 24 440 30 451 2.28e-83 263
MS.gene87537.t1 MTR_7g026850 69.461 167 50 1 29 194 12 178 3.90e-81 248
MS.gene87537.t1 MTR_7g026770 59.474 190 48 2 141 329 132 293 1.65e-70 225
MS.gene87537.t1 MTR_3g070170 32.489 474 262 12 3 436 11 466 2.64e-62 209
MS.gene87537.t1 MTR_4g015180 32.577 485 249 11 16 439 19 486 9.59e-61 205
MS.gene87537.t1 MTR_5g081960 31.601 481 252 10 16 436 19 482 1.31e-60 205
MS.gene87537.t1 MTR_4g094825 48.500 200 95 5 5 198 72 269 2.61e-54 186
MS.gene87537.t1 MTR_4g094825 57.823 147 49 2 292 436 252 387 1.00e-48 171
MS.gene87537.t1 MTR_6g045553 30.820 451 276 10 16 436 27 471 3.26e-54 187
MS.gene87537.t1 MTR_3g102030 34.713 314 164 7 87 396 44 320 1.62e-50 174
MS.gene87537.t1 MTR_6g034495 61.832 131 40 3 60 180 34 164 3.74e-48 162
MS.gene87537.t1 MTR_6g034485 57.576 132 48 2 188 319 2 125 1.16e-45 154
MS.gene87537.t1 MTR_7g026800 50.292 171 54 4 211 379 19 160 1.24e-38 138
MS.gene87537.t1 MTR_4g022780 49.701 167 54 3 211 376 20 157 4.28e-38 136
MS.gene87537.t1 MTR_7g026830 71.429 84 24 0 82 165 14 97 1.33e-36 130
MS.gene87537.t1 MTR_7g026780 56.364 110 24 2 331 440 26 111 7.11e-31 115
MS.gene87537.t1 MTR_8g096530 30.028 353 214 12 49 391 53 382 8.50e-30 120
MS.gene87537.t1 MTR_8g075610 27.226 393 242 16 55 429 47 413 1.45e-28 117
MS.gene87537.t1 MTR_4g007540 30.084 359 215 16 55 398 56 393 6.84e-28 115
MS.gene87537.t1 MTR_3g098980 27.704 379 236 13 55 421 66 418 2.66e-27 113
MS.gene87537.t1 MTR_2g064495 27.604 384 241 15 55 422 56 418 1.73e-26 111
MS.gene87537.t1 MTR_2g105330 27.344 384 242 15 55 422 56 418 2.03e-26 110
MS.gene87537.t1 MTR_3g026950 26.302 384 210 16 52 392 51 404 2.14e-22 99.4
MS.gene87537.t1 MTR_1g081660 26.303 403 226 15 24 392 31 396 2.52e-22 99.4
MS.gene87537.t1 MTR_7g014250 25.056 447 278 16 22 431 24 450 8.06e-21 94.7
MS.gene87537.t1 MTR_3g071320 25.115 434 291 14 17 436 19 432 1.14e-20 94.4
MS.gene87537.t1 MTR_4g013050 26.016 369 188 12 53 392 51 363 1.35e-20 93.6
MS.gene87537.t1 MTR_2g089650 24.396 414 266 18 38 417 43 443 4.17e-20 92.8
MS.gene87537.t1 MTR_7g014330 25.176 425 261 16 18 405 20 424 8.97e-20 91.7
MS.gene87537.t1 MTR_7g026870 74.074 54 14 0 297 350 98 151 9.15e-20 85.9
MS.gene87537.t1 MTR_7g015860 23.786 412 249 14 22 392 26 413 1.23e-18 88.2
MS.gene87537.t1 MTR_7g114520 24.722 360 240 13 44 392 53 392 2.07e-18 87.8
MS.gene87537.t1 MTR_5g076700 23.832 428 272 16 25 422 24 427 2.98e-18 87.0
MS.gene87537.t1 MTR_3g086020 25.823 395 244 16 55 440 61 415 3.00e-18 86.7
MS.gene87537.t1 MTR_3g049150 24.823 282 171 8 138 391 131 399 3.11e-18 87.0
MS.gene87537.t1 MTR_7g026880 68.627 51 16 0 389 439 2 52 3.99e-18 78.2
MS.gene87537.t1 MTR_3g112180 25.248 404 245 18 49 427 55 426 4.76e-18 86.7
MS.gene87537.t1 MTR_7g114550 23.583 441 284 16 25 436 24 440 5.41e-18 86.3
MS.gene87537.t1 MTR_1g053315 24.048 420 256 18 33 422 60 446 2.17e-17 84.3
MS.gene87537.t1 MTR_7g014230 25.346 434 249 15 18 405 22 426 3.10e-17 84.0
MS.gene87537.t1 MTR_6g015320 25.641 312 202 10 137 427 142 444 4.42e-17 83.6
MS.gene87537.t1 MTR_4g097090 23.833 407 274 13 55 439 65 457 4.89e-17 83.6
MS.gene87537.t1 MTR_7g114470 23.898 431 269 18 25 422 25 429 9.40e-17 82.4
MS.gene87537.t1 MTR_7g014200 22.799 443 293 12 22 427 28 458 2.07e-16 81.6
MS.gene87537.t1 MTR_7g084940 26.950 282 179 10 53 323 67 332 3.67e-16 80.5
MS.gene87537.t1 MTR_6g015315 24.684 316 207 10 137 430 139 445 4.66e-16 80.5
MS.gene87537.t1 MTR_2g089765 24.515 412 243 14 26 391 45 434 4.81e-16 80.5
MS.gene87537.t1 MTR_7g027175 24.870 386 253 14 55 427 68 429 4.83e-16 80.1
MS.gene87537.t1 MTR_2g089735 25.472 424 256 17 41 424 43 446 5.92e-16 80.1
MS.gene87537.t1 MTR_2g089670 23.741 417 243 16 21 391 42 429 8.89e-16 79.7
MS.gene87537.t1 MTR_4g055170 27.815 302 197 11 55 350 69 355 1.48e-15 79.0
MS.gene87537.t1 MTR_8g012750 23.267 404 271 10 22 391 24 422 2.66e-15 78.2
MS.gene87537.t1 MTR_6g015830 24.829 439 291 13 17 429 27 452 2.76e-15 78.2
MS.gene87537.t1 MTR_6g015815 21.493 442 292 14 22 429 32 452 4.52e-15 77.4
MS.gene87537.t1 MTR_7g084910 27.407 270 162 9 53 307 68 318 6.07e-15 77.0
MS.gene87537.t1 MTR_6g015850 23.380 432 291 12 22 428 32 448 1.32e-14 75.9
MS.gene87537.t1 MTR_7g085930 26.165 279 187 6 43 308 47 319 1.62e-14 75.9
MS.gene87537.t1 MTR_5g084870 23.148 432 271 15 5 413 4 397 1.89e-14 75.5
MS.gene87537.t1 MTR_1g040195 24.645 422 260 18 25 427 23 405 2.97e-14 74.7
MS.gene87537.t1 MTR_6g053810 25.806 279 184 10 55 323 71 336 4.10e-14 74.3
MS.gene87537.t1 MTR_7g014610 25.185 405 250 15 55 421 68 457 4.65e-14 74.3
MS.gene87537.t1 MTR_1g040215 23.721 430 260 18 8 408 13 403 1.18e-13 73.2
MS.gene87537.t1 MTR_8g079950 25.337 371 236 14 41 396 42 386 2.99e-13 71.6
MS.gene87537.t1 MTR_5g090810 24.130 431 263 19 2 392 6 412 3.93e-13 71.2
MS.gene87537.t1 MTR_7g014360 26.692 266 175 8 138 395 90 343 1.50e-12 69.3
MS.gene87537.t1 MTR_5g090830 24.362 431 262 19 2 392 6 412 1.60e-12 69.7
MS.gene87537.t1 MTR_7g086000 26.202 416 255 18 58 439 63 460 3.06e-12 68.6
MS.gene87537.t1 MTR_7g085990 25.806 279 188 6 43 308 43 315 5.71e-12 67.8
MS.gene87537.t1 MTR_5g090880 24.457 368 230 16 55 393 67 415 1.13e-11 67.0
MS.gene87537.t1 MTR_3g450710 26.613 372 220 18 52 392 62 411 1.54e-11 66.6
MS.gene87537.t1 MTR_3g450690 26.829 369 220 18 52 392 69 415 2.14e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87537.t1 AT5G67150 43.119 436 225 11 16 439 21 445 6.23e-112 337
MS.gene87537.t1 AT3G50300 41.839 435 228 11 1 421 1 424 8.76e-100 305
MS.gene87537.t1 AT3G50280 40.139 431 240 8 16 437 19 440 4.75e-98 301
MS.gene87537.t1 AT5G67160 38.426 432 237 10 17 440 22 432 1.15e-92 287
MS.gene87537.t1 AT3G50270 37.748 453 260 10 2 440 4 448 2.21e-91 284
MS.gene87537.t1 AT5G42830 36.891 431 247 10 24 440 30 449 3.32e-84 265
MS.gene87537.t1 AT5G38130 39.141 419 227 12 16 421 39 442 4.31e-84 266
MS.gene87537.t1 AT5G07860 37.471 435 236 10 24 439 31 448 3.13e-80 255
MS.gene87537.t1 AT5G07850 36.842 437 242 12 24 440 31 453 1.42e-74 241
MS.gene87537.t1 AT5G07870 37.825 423 237 10 38 440 45 461 1.05e-73 239
MS.gene87537.t1 AT2G39980 33.264 478 246 13 16 439 21 479 6.78e-68 224
MS.gene87537.t1 AT3G50280 38.871 319 180 5 125 437 1 310 1.67e-66 215
MS.gene87537.t1 AT5G01210 34.328 469 246 13 16 440 19 469 1.54e-64 215
MS.gene87537.t1 AT5G23940 32.751 458 269 13 5 436 4 448 2.58e-64 214
MS.gene87537.t1 AT3G50290 40.876 274 148 7 122 389 4 269 6.64e-51 173
MS.gene87537.t1 AT5G48930 27.989 368 215 10 53 398 54 393 2.21e-28 116
MS.gene87537.t1 AT3G29670 24.883 426 279 14 20 421 25 433 1.23e-24 106
MS.gene87537.t1 AT3G48720 27.198 364 230 12 42 392 44 385 2.39e-24 105
MS.gene87537.t1 AT3G29590 26.058 449 299 17 3 431 7 442 1.40e-23 102
MS.gene87537.t1 AT2G19070 24.837 459 272 21 27 438 19 451 2.26e-22 99.4
MS.gene87537.t1 AT5G41040 28.011 357 218 16 50 392 64 395 5.66e-22 98.2
MS.gene87537.t1 AT5G41040 28.011 357 218 16 50 392 80 411 6.89e-22 98.2
MS.gene87537.t1 AT5G57840 27.873 409 242 18 41 421 39 422 6.98e-20 92.0
MS.gene87537.t1 AT5G39080 25.743 404 246 18 52 421 62 445 1.39e-19 91.3
MS.gene87537.t1 AT3G29635 24.541 436 269 16 22 421 27 438 6.96e-19 89.0
MS.gene87537.t1 AT5G63560 26.478 389 239 16 50 422 56 413 1.19e-18 88.2
MS.gene87537.t1 AT3G29680 25.628 398 249 15 49 421 56 431 1.84e-18 87.8
MS.gene87537.t1 AT1G24430 24.424 434 263 19 26 440 38 425 2.88e-18 87.0
MS.gene87537.t1 AT5G39050 24.282 383 226 13 66 414 90 442 3.49e-18 87.0
MS.gene87537.t1 AT3G26040 24.029 412 258 17 49 440 53 429 1.01e-17 85.5
MS.gene87537.t1 AT2G40230 24.645 422 287 16 22 431 24 426 1.44e-17 85.1
MS.gene87537.t1 AT1G28680 24.874 398 249 15 58 440 65 427 3.98e-16 80.5
MS.gene87537.t1 AT5G07080 27.027 296 183 13 42 321 53 331 7.66e-16 79.7
MS.gene87537.t1 AT5G39090 26.642 274 170 12 135 391 140 399 2.03e-15 78.6
MS.gene87537.t1 AT1G03390 25.490 408 255 19 42 422 59 444 2.09e-15 78.6
MS.gene87537.t1 AT5G61160 22.878 271 190 9 135 392 139 403 1.92e-14 75.5
MS.gene87537.t1 AT5G17540 25.792 442 269 21 25 434 23 437 6.35e-14 73.9
MS.gene87537.t1 AT1G28680 24.579 297 203 9 58 348 65 346 1.07e-13 72.8
MS.gene87537.t1 AT5G23970 26.444 450 279 21 4 436 3 417 1.10e-13 73.2
MS.gene87537.t1 AT3G30280 23.733 375 212 17 54 398 61 391 1.44e-13 72.8
MS.gene87537.t1 AT5G16410 22.558 430 286 15 1 413 45 444 5.18e-13 71.2
MS.gene87537.t1 AT1G65450 25.352 284 188 12 53 324 70 341 9.31e-13 70.1
MS.gene87537.t1 AT2G23510 24.611 386 255 18 55 422 69 436 1.64e-12 69.3
MS.gene87537.t1 AT1G32910 25.606 371 211 18 53 392 63 399 1.81e-12 69.3
MS.gene87537.t1 AT1G03940 22.772 404 278 11 50 428 61 455 1.84e-12 69.3
MS.gene87537.t1 AT3G47170 25.839 298 184 11 44 321 64 344 3.78e-12 68.6
MS.gene87537.t1 AT2G25150 25.414 362 234 14 50 395 62 403 9.72e-12 67.0
MS.gene87537.t1 AT4G15390 22.277 404 259 15 10 394 24 391 7.03e-11 64.3
MS.gene87537.t1 AT4G15390 22.277 404 259 15 10 394 24 391 7.03e-11 64.3

Find 82 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCATGGTTTGATGTTTA+TGG 0.113342 4.1:-75558515 MS.gene87537:CDS
GTGTCTTCCTTACAAGATTT+TGG 0.202537 4.1:-75558384 MS.gene87537:CDS
CCATTGCCAGAAGATTATTT+TGG 0.248348 4.1:-75558746 MS.gene87537:CDS
GATGTTTATGGTAATGATTT+TGG 0.308658 4.1:-75558503 MS.gene87537:CDS
TGGTTGGATAAAGTAGAAAT+TGG 0.327409 4.1:+75559554 None:intergenic
GCGCTTAATACGTTCATAAT+AGG 0.328958 4.1:-75558791 MS.gene87537:CDS
TGATCATGAATTCATGGATA+TGG 0.332225 4.1:-75558348 MS.gene87537:CDS
TTCTTGTGGATCAAGATGTT+TGG 0.349905 4.1:+75558817 None:intergenic
AATGAAAGCTGGGGAGTTGT+TGG 0.360707 4.1:-75558696 MS.gene87537:CDS
GAAACCAACGCTCTAGTGTT+GGG 0.360883 4.1:+75559080 None:intergenic
TTTCATTGCTTTGATTGTAT+TGG 0.364692 4.1:+75559615 None:intergenic
GGAATGGTTTGTGCCTTTGC+TGG 0.368156 4.1:-75558437 MS.gene87537:CDS
TATGTCTTAGCCGTTGAATT+TGG 0.371174 4.1:+75559491 None:intergenic
TGAGAAATCCAAGCTTCATT+AGG 0.373854 4.1:-75558580 MS.gene87537:CDS
GCGTTTCCAAAATAATCTTC+TGG 0.379778 4.1:+75558740 None:intergenic
GCCAGCAAGAGGTTCGAAAA+AGG 0.381613 4.1:+75559450 None:intergenic
CTAGCTGACGGCATCTTCAT+AGG 0.398680 4.1:-75559205 MS.gene87537:CDS
GGGAGTTGTTGGAGGAAGGT+GGG 0.400037 4.1:-75558685 MS.gene87537:CDS
GAAACGCGATTATAGGTTGC+GGG 0.410719 4.1:-75558724 MS.gene87537:CDS
ATGGGCTGTTGAATACCATT+TGG 0.419783 4.1:+75559058 None:intergenic
TTGTATTGGTGGAGAGTACT+TGG 0.455345 4.1:+75559629 None:intergenic
AAACCATGGAGAACTGCTAA+TGG 0.455624 4.1:+75558526 None:intergenic
GGGAAATGATCATGAATTCA+TGG 0.457595 4.1:-75558354 MS.gene87537:CDS
TTGTTGGAGGAAGGTGGGAT+TGG 0.457605 4.1:-75558680 MS.gene87537:CDS
GGGGAGTTGTTGGAGGAAGG+TGG 0.459892 4.1:-75558686 MS.gene87537:CDS
CGGAGTGGAAATAGAACAAA+TGG 0.464324 4.1:-75558458 MS.gene87537:CDS
TGCGGGTTTAAAATGAAAGC+TGG 0.465815 4.1:-75558707 MS.gene87537:CDS
GGCAATGGTGGAACAAGTCT+TGG 0.472119 4.1:+75558761 None:intergenic
CCAAAATAATCTTCTGGCAA+TGG 0.477773 4.1:+75558746 None:intergenic
GCTGGGGAGTTGTTGGAGGA+AGG 0.483907 4.1:-75558689 MS.gene87537:CDS
AAAACCCACATATGTTCCTT+CGG 0.485243 4.1:-75559311 MS.gene87537:CDS
GAGGAAGGTGGGATTGGTAA+AGG 0.486075 4.1:-75558674 MS.gene87537:CDS
CATTGCTTTGATTGTATTGG+TGG 0.486507 4.1:+75559618 None:intergenic
CTATCAAGCCTAATGAAGCT+TGG 0.491157 4.1:+75558572 None:intergenic
GGGGAAAGCCTGTGGCAGTT+CGG 0.491671 4.1:-75558478 MS.gene87537:CDS
ATAAAATTGATTTAACTCCA+TGG 0.501405 4.1:-75559582 MS.gene87537:CDS
ATTGCCTCCAAAATCTTGTA+AGG 0.504306 4.1:+75558377 None:intergenic
TAAAATTGATTTAACTCCAT+GGG 0.505681 4.1:-75559581 MS.gene87537:CDS
GGAAACCAACGCTCTAGTGT+TGG 0.511281 4.1:+75559079 None:intergenic
GCCTTTGCTGGAATAGAAGA+AGG 0.511653 4.1:-75558425 MS.gene87537:CDS
TTCAATCATGTTGTAGCTGA+CGG 0.511810 4.1:-75559175 MS.gene87537:CDS
TATAGTTAAAGGAAATCGTA+TGG 0.515144 4.1:+75559039 None:intergenic
CTAGAGCGTTGGTTTCCAAA+TGG 0.515960 4.1:-75559073 MS.gene87537:CDS
TTTCCACTCCGAACTGCCAC+AGG 0.522499 4.1:+75558470 None:intergenic
TGAATTTGGTCTGATGCTAC+AGG 0.526430 4.1:+75559505 None:intergenic
TGTAAGGAAGACACACTTGA+AGG 0.538009 4.1:+75558393 None:intergenic
TAAAGAATGATTATGAGTCA+TGG 0.538860 4.1:-75558604 MS.gene87537:CDS
GCGGGTTTAAAATGAAAGCT+GGG 0.538973 4.1:-75558706 MS.gene87537:CDS
GTATTAAGCGCATCTTCTTG+TGG 0.540410 4.1:+75558803 None:intergenic
AGGTACATCAAAATCATTGT+TGG 0.540953 4.1:-75559245 MS.gene87537:CDS
GAAAGCTGGGGAGTTGTTGG+AGG 0.541663 4.1:-75558693 MS.gene87537:CDS
TGGCCATTAGCAGTTCTCCA+TGG 0.541677 4.1:-75558529 MS.gene87537:CDS
AAATAATCTTCTGGCAATGG+TGG 0.542517 4.1:+75558749 None:intergenic
GGAAACGCGATTATAGGTTG+CGG 0.544739 4.1:-75558725 MS.gene87537:CDS
TAGTTTGTAACTCCGTTGAA+AGG 0.548129 4.1:+75559271 None:intergenic
CGTGTTCTGTAACTTTGAGA+CGG 0.548497 4.1:+75559428 None:intergenic
TGGAAATAGAACAAATGGAA+TGG 0.553380 4.1:-75558453 MS.gene87537:CDS
ACCTTCTTCTATTCCAGCAA+AGG 0.555908 4.1:+75558424 None:intergenic
AGTAGAAATTGGAGATCCCA+TGG 0.565431 4.1:+75559565 None:intergenic
AAACCAACGCTCTAGTGTTG+GGG 0.569521 4.1:+75559081 None:intergenic
ATGGCATTTCATCAATTCGT+GGG 0.570140 4.1:-75559143 MS.gene87537:CDS
AACAACCGAAGGAACATATG+TGG 0.575430 4.1:+75559306 None:intergenic
ATAGTTAAAGGAAATCGTAT+GGG 0.585010 4.1:+75559040 None:intergenic
CTACAGGATGGTGATAAAGA+AGG 0.592406 4.1:+75559521 None:intergenic
CATGGCATTTCATCAATTCG+TGG 0.610417 4.1:-75559144 MS.gene87537:CDS
ACTTTGAGACGGCCAGCAAG+AGG 0.616523 4.1:+75559439 None:intergenic
TCTGTAACATGCAATAATGC+AGG 0.619827 4.1:-75559385 MS.gene87537:CDS
ATTGTTGGCAGTTCAAGTGA+CGG 0.619862 4.1:-75559230 MS.gene87537:CDS
AACCAACGCTCTAGTGTTGG+GGG 0.632370 4.1:+75559082 None:intergenic
TAGCTGACGGACAGTCCACA+TGG 0.634434 4.1:-75559162 MS.gene87537:CDS
CAAGTGACGGAGCTAGCTGA+CGG 0.639821 4.1:-75559217 MS.gene87537:CDS
CGGGTTTAAAATGAAAGCTG+GGG 0.642028 4.1:-75558705 MS.gene87537:CDS
AAGCCTGTGGCAGTTCGGAG+TGG 0.647498 4.1:-75558473 MS.gene87537:CDS
ACAACCGAAGGAACATATGT+GGG 0.650889 4.1:+75559307 None:intergenic
AACGGAGTTACAAACTACGA+AGG 0.656439 4.1:-75559265 MS.gene87537:CDS
TTACCATAAACATCAAACCA+TGG 0.659822 4.1:+75558512 None:intergenic
TAGCATCAGACCAAATTCAA+CGG 0.663707 4.1:-75559501 MS.gene87537:CDS
AGGTGTAAACTTTATACATG+CGG 0.677512 4.1:-75559365 MS.gene87537:CDS
GCCACAAAAGAATTACTCTG+AGG 0.711684 4.1:-75559014 MS.gene87537:CDS
CGAATTGATGAAATGCCATG+TGG 0.718082 4.1:+75559147 None:intergenic
TTTGGTCTGATGCTACAGGA+TGG 0.756053 4.1:+75559509 None:intergenic
TCGTGGGCTGAAATGTCACG+TGG 0.758657 4.1:-75559127 MS.gene87537:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAAATTGATTTAACTCCA+TGG - chr4.1:75558398-75558417 MS.gene87537:CDS 20.0%
!!! TAAAATTGATTTAACTCCAT+GGG - chr4.1:75558399-75558418 MS.gene87537:CDS 20.0%
!!! TTGTAGAGAAGATATTTTTT+TGG + chr4.1:75559106-75559125 None:intergenic 20.0%
! AAAAATTCTATCTATCTGAG+AGG - chr4.1:75559016-75559035 MS.gene87537:CDS 25.0%
! ATAGTTAAAGGAAATCGTAT+GGG + chr4.1:75558943-75558962 None:intergenic 25.0%
! TAAAGAATGATTATGAGTCA+TGG - chr4.1:75559376-75559395 MS.gene87537:CDS 25.0%
! TATAGTTAAAGGAAATCGTA+TGG + chr4.1:75558944-75558963 None:intergenic 25.0%
! TTTCATTGCTTTGATTGTAT+TGG + chr4.1:75558368-75558387 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG - chr4.1:75559477-75559496 MS.gene87537:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG - chr4.1:75559482-75559501 MS.gene87537:CDS 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG - chr4.1:75559481-75559500 MS.gene87537:CDS 25.0%
AGGTACATCAAAATCATTGT+TGG - chr4.1:75558735-75558754 MS.gene87537:CDS 30.0%
AGGTGTAAACTTTATACATG+CGG - chr4.1:75558615-75558634 MS.gene87537:CDS 30.0%
CTTTATCCAACCAACAAAAA+AGG - chr4.1:75558436-75558455 MS.gene87537:CDS 30.0%
TGATCATGAATTCATGGATA+TGG - chr4.1:75559632-75559651 MS.gene87537:CDS 30.0%
TGGAAATAGAACAAATGGAA+TGG - chr4.1:75559527-75559546 MS.gene87537:CDS 30.0%
TGGTTGGATAAAGTAGAAAT+TGG + chr4.1:75558429-75558448 None:intergenic 30.0%
TTACCATAAACATCAAACCA+TGG + chr4.1:75559471-75559490 None:intergenic 30.0%
!! TATGGTAATGATTTTGGATG+GGG - chr4.1:75559483-75559502 MS.gene87537:CDS 30.0%
!! TATTTTGGAAACGCGATTAT+AGG - chr4.1:75559249-75559268 MS.gene87537:CDS 30.0%
!! TTTTGTGGCTCTATAGTTAA+AGG + chr4.1:75558955-75558974 None:intergenic 30.0%
!!! ATAAAGAAGGCCTTTTTTGT+TGG + chr4.1:75558449-75558468 None:intergenic 30.0%
!!! CGTTCATTTTTTCCTTTCAA+CGG - chr4.1:75558697-75558716 MS.gene87537:CDS 30.0%
!!! CTTGAGCACTAAGAATTTTT+TGG - chr4.1:75559431-75559450 MS.gene87537:CDS 30.0%
AAAAATGAACGAACAACCGA+AGG + chr4.1:75558688-75558707 None:intergenic 35.0%
AAAACCCACATATGTTCCTT+CGG - chr4.1:75558669-75558688 MS.gene87537:CDS 35.0%
AAATAATCTTCTGGCAATGG+TGG + chr4.1:75559234-75559253 None:intergenic 35.0%
AAGAGGTTCGAAAAAGGAAA+GGG + chr4.1:75558527-75558546 None:intergenic 35.0%
ATGGCATTTCATCAATTCGT+GGG - chr4.1:75558837-75558856 MS.gene87537:CDS 35.0%
ATTGCCTCCAAAATCTTGTA+AGG + chr4.1:75559606-75559625 None:intergenic 35.0%
CATTGCTTTGATTGTATTGG+TGG + chr4.1:75558365-75558384 None:intergenic 35.0%
CCAAAATAATCTTCTGGCAA+TGG + chr4.1:75559237-75559256 None:intergenic 35.0%
GCGCTTAATACGTTCATAAT+AGG - chr4.1:75559189-75559208 MS.gene87537:CDS 35.0%
GCGTTTCCAAAATAATCTTC+TGG + chr4.1:75559243-75559262 None:intergenic 35.0%
GGGAAATGATCATGAATTCA+TGG - chr4.1:75559626-75559645 MS.gene87537:CDS 35.0%
TAGCATCAGACCAAATTCAA+CGG - chr4.1:75558479-75558498 MS.gene87537:CDS 35.0%
TATGTCTTAGCCGTTGAATT+TGG + chr4.1:75558492-75558511 None:intergenic 35.0%
TCTCCATGGTTTGATGTTTA+TGG - chr4.1:75559465-75559484 MS.gene87537:CDS 35.0%
TCTGTAACATGCAATAATGC+AGG - chr4.1:75558595-75558614 MS.gene87537:CDS 35.0%
TGAGAAATCCAAGCTTCATT+AGG - chr4.1:75559400-75559419 MS.gene87537:CDS 35.0%
TTCAATCATGTTGTAGCTGA+CGG - chr4.1:75558805-75558824 MS.gene87537:CDS 35.0%
TTCTTGTGGATCAAGATGTT+TGG + chr4.1:75559166-75559185 None:intergenic 35.0%
! AAGGAACATATGTGGGTTTT+AGG + chr4.1:75558669-75558688 None:intergenic 35.0%
! CCATTGCCAGAAGATTATTT+TGG - chr4.1:75559234-75559253 MS.gene87537:CDS 35.0%
! GTGTCTTCCTTACAAGATTT+TGG - chr4.1:75559596-75559615 MS.gene87537:CDS 35.0%
! TAGTTTGTAACTCCGTTGAA+AGG + chr4.1:75558712-75558731 None:intergenic 35.0%
! TCCTCAGAGTAATTCTTTTG+TGG + chr4.1:75558970-75558989 None:intergenic 35.0%
! TCTTCCTTACAAGATTTTGG+AGG - chr4.1:75559599-75559618 MS.gene87537:CDS 35.0%
!! AAGCGCTTTTAACTCATCTT+TGG - chr4.1:75559124-75559143 MS.gene87537:CDS 35.0%
!! TACAAGATTTTGGAGGCAAT+GGG - chr4.1:75559606-75559625 MS.gene87537:CDS 35.0%
!! TTACAAGATTTTGGAGGCAA+TGG - chr4.1:75559605-75559624 MS.gene87537:CDS 35.0%
AAACCATGGAGAACTGCTAA+TGG + chr4.1:75559457-75559476 None:intergenic 40.0%
AACAACCGAAGGAACATATG+TGG + chr4.1:75558677-75558696 None:intergenic 40.0%
AACGGAGTTACAAACTACGA+AGG - chr4.1:75558715-75558734 MS.gene87537:CDS 40.0%
ACAACCGAAGGAACATATGT+GGG + chr4.1:75558676-75558695 None:intergenic 40.0%
ACCTTCTTCTATTCCAGCAA+AGG + chr4.1:75559559-75559578 None:intergenic 40.0%
AGTAGAAATTGGAGATCCCA+TGG + chr4.1:75558418-75558437 None:intergenic 40.0%
ATTGTTGGCAGTTCAAGTGA+CGG - chr4.1:75558750-75558769 MS.gene87537:CDS 40.0%
CAAGAGGTTCGAAAAAGGAA+AGG + chr4.1:75558528-75558547 None:intergenic 40.0%
CATGGCATTTCATCAATTCG+TGG - chr4.1:75558836-75558855 MS.gene87537:CDS 40.0%
CGAATTGATGAAATGCCATG+TGG + chr4.1:75558836-75558855 None:intergenic 40.0%
CGGAGTGGAAATAGAACAAA+TGG - chr4.1:75559522-75559541 MS.gene87537:CDS 40.0%
CTACAGGATGGTGATAAAGA+AGG + chr4.1:75558462-75558481 None:intergenic 40.0%
CTATCAAGCCTAATGAAGCT+TGG + chr4.1:75559411-75559430 None:intergenic 40.0%
GCCACAAAAGAATTACTCTG+AGG - chr4.1:75558966-75558985 MS.gene87537:CDS 40.0%
GTATTAAGCGCATCTTCTTG+TGG + chr4.1:75559180-75559199 None:intergenic 40.0%
TGAATTTGGTCTGATGCTAC+AGG + chr4.1:75558478-75558497 None:intergenic 40.0%
TGTAAGGAAGACACACTTGA+AGG + chr4.1:75559590-75559609 None:intergenic 40.0%
! ATGGGCTGTTGAATACCATT+TGG + chr4.1:75558925-75558944 None:intergenic 40.0%
! CGGGTTTAAAATGAAAGCTG+GGG - chr4.1:75559275-75559294 MS.gene87537:CDS 40.0%
! CGTGTTCTGTAACTTTGAGA+CGG + chr4.1:75558555-75558574 None:intergenic 40.0%
! GCGGGTTTAAAATGAAAGCT+GGG - chr4.1:75559274-75559293 MS.gene87537:CDS 40.0%
! TGCGGGTTTAAAATGAAAGC+TGG - chr4.1:75559273-75559292 MS.gene87537:CDS 40.0%
!! TTGTATTGGTGGAGAGTACT+TGG + chr4.1:75558354-75558373 None:intergenic 40.0%
!!! AGAAGGCCTTTTTTGTTGGT+TGG + chr4.1:75558445-75558464 None:intergenic 40.0%
AAACCAACGCTCTAGTGTTG+GGG + chr4.1:75558902-75558921 None:intergenic 45.0%
AATGAAAGCTGGGGAGTTGT+TGG - chr4.1:75559284-75559303 MS.gene87537:CDS 45.0%
GAAACCAACGCTCTAGTGTT+GGG + chr4.1:75558903-75558922 None:intergenic 45.0%
GAAACGCGATTATAGGTTGC+GGG - chr4.1:75559256-75559275 MS.gene87537:CDS 45.0%
GCCTTTGCTGGAATAGAAGA+AGG - chr4.1:75559555-75559574 MS.gene87537:CDS 45.0%
GGAAACGCGATTATAGGTTG+CGG - chr4.1:75559255-75559274 MS.gene87537:CDS 45.0%
TTTGGTCTGATGCTACAGGA+TGG + chr4.1:75558474-75558493 None:intergenic 45.0%
!! CTAGAGCGTTGGTTTCCAAA+TGG - chr4.1:75558907-75558926 MS.gene87537:CDS 45.0%
!!! TCCTTTTTCGAACCTCTTGC+TGG - chr4.1:75558529-75558548 MS.gene87537:CDS 45.0%
CTAGCTGACGGCATCTTCAT+AGG - chr4.1:75558775-75558794 MS.gene87537:CDS 50.0%
GCCAGCAAGAGGTTCGAAAA+AGG + chr4.1:75558533-75558552 None:intergenic 50.0%
GGAAACCAACGCTCTAGTGT+TGG + chr4.1:75558904-75558923 None:intergenic 50.0%
TGGCCATTAGCAGTTCTCCA+TGG - chr4.1:75559451-75559470 MS.gene87537:CDS 50.0%
TTGTTGGAGGAAGGTGGGAT+TGG - chr4.1:75559300-75559319 MS.gene87537:CDS 50.0%
! AACCAACGCTCTAGTGTTGG+GGG + chr4.1:75558901-75558920 None:intergenic 50.0%
! GAGGAAGGTGGGATTGGTAA+AGG - chr4.1:75559306-75559325 MS.gene87537:CDS 50.0%
! GGAATGGTTTGTGCCTTTGC+TGG - chr4.1:75559543-75559562 MS.gene87537:CDS 50.0%
! GGCAATGGTGGAACAAGTCT+TGG + chr4.1:75559222-75559241 None:intergenic 50.0%
! TTTTGGATGGGGAAAGCCTG+TGG - chr4.1:75559494-75559513 MS.gene87537:CDS 50.0%
AACCCCCAACACTAGAGCGT+TGG - chr4.1:75558896-75558915 MS.gene87537:CDS 55.0%
GAAAGCTGGGGAGTTGTTGG+AGG - chr4.1:75559287-75559306 MS.gene87537:CDS 55.0%
GGGAGTTGTTGGAGGAAGGT+GGG - chr4.1:75559295-75559314 MS.gene87537:CDS 55.0%
TTTCCACTCCGAACTGCCAC+AGG + chr4.1:75559513-75559532 None:intergenic 55.0%
! CAAGTGACGGAGCTAGCTGA+CGG - chr4.1:75558763-75558782 MS.gene87537:CDS 55.0%
! TAGCTGACGGACAGTCCACA+TGG - chr4.1:75558818-75558837 MS.gene87537:CDS 55.0%
! TCGTGGGCTGAAATGTCACG+TGG - chr4.1:75558853-75558872 MS.gene87537:CDS 55.0%
!! ACTTTGAGACGGCCAGCAAG+AGG + chr4.1:75558544-75558563 None:intergenic 55.0%
AAGCCTGTGGCAGTTCGGAG+TGG - chr4.1:75559507-75559526 MS.gene87537:CDS 60.0%
GCTGGGGAGTTGTTGGAGGA+AGG - chr4.1:75559291-75559310 MS.gene87537:CDS 60.0%
GGGGAAAGCCTGTGGCAGTT+CGG - chr4.1:75559502-75559521 MS.gene87537:CDS 60.0%
GGGGAGTTGTTGGAGGAAGG+TGG - chr4.1:75559294-75559313 MS.gene87537:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 75558340 75559662 75558340 ID=MS.gene87537
chr4.1 mRNA 75558340 75559662 75558340 ID=MS.gene87537.t1;Parent=MS.gene87537
chr4.1 exon 75558340 75559662 75558340 ID=MS.gene87537.t1.exon1;Parent=MS.gene87537.t1
chr4.1 CDS 75558340 75559662 75558340 ID=cds.MS.gene87537.t1;Parent=MS.gene87537.t1
Gene Sequence

>MS.gene87537

ATGGGTGCATTCCAAGTACTCTCCACCAATACAATCAAAGCAATGAAATCAAGTAATCATAAAATTGATTTAACTCCATGGGATCTCCAATTTCTACTTTATCCAACCAACAAAAAAGGCCTTCTTTATCACCATCCTGTAGCATCAGACCAAATTCAACGGCTAAGACATACTCTTTCTTCTGCCCTTTCCTTTTTCGAACCTCTTGCTGGCCGTCTCAAAGTTACAGAACACGAAGACAGTACTGTCTCGTGTTCTGTAACATGCAATAATGCAGGTGTAAACTTTATACATGCGGCAGCAAAGAATACTTGTGTTGCTGACATCCTAAAACCCACATATGTTCCTTCGGTTGTTCGTTCATTTTTTCCTTTCAACGGAGTTACAAACTACGAAGGTACATCAAAATCATTGTTGGCAGTTCAAGTGACGGAGCTAGCTGACGGCATCTTCATAGGCTGTAATTTCAATCATGTTGTAGCTGACGGACAGTCCACATGGCATTTCATCAATTCGTGGGCTGAAATGTCACGTGGTTGTCATCATCAAATATCTAAACCCCCAACACTAGAGCGTTGGTTTCCAAATGGTATTCAACAGCCCATACGATTTCCTTTAACTATAGAGCCACAAAAGAATTACTCTGAGGAGTTGAGTTTTTCGTCATCTGATGACGAAAAATTCTATCTATCTGAGAGGATATTTCACTTCACAAAGAAGAAAATCATGCAATTGAAATCAAAAGTCAACGCAGCGATTGATACCAAAAAAATATCTTCTCTACAAGCGCTTTTAACTCATCTTTGGTGTTCAGTGATTCGTTCCAAACATCTTGATCCACAAGAAGATGCGCTTAATACGTTCATAATAGGAGTTAGACCAAGACTTGTTCCACCATTGCCAGAAGATTATTTTGGAAACGCGATTATAGGTTGCGGGTTTAAAATGAAAGCTGGGGAGTTGTTGGAGGAAGGTGGGATTGGTAAAGGTGCTTTGTTGATGAATAAGTTAATTGCTTTGCACTCTAATGAGAAATTAAAGAATGATTATGAGTCATGGTTGAGAAATCCAAGCTTCATTAGGCTTGATAGCTTGAGCACTAAGAATTTTTTGGCCATTAGCAGTTCTCCATGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAGCCTGTGGCAGTTCGGAGTGGAAATAGAACAAATGGAATGGTTTGTGCCTTTGCTGGAATAGAAGAAGGTAGCATTGACCTTCAAGTGTGTCTTCCTTACAAGATTTTGGAGGCAATGGGAAATGATCATGAATTCATGGATATGGTTAACTAA
Protein sequence

>MS.gene87537.t1

MGAFQVLSTNTIKAMKSSNHKIDLTPWDLQFLLYPTNKKGLLYHHPVASDQIQRLRHTLSSALSFFEPLAGRLKVTEHEDSTVSCSVTCNNAGVNFIHAAAKNTCVADILKPTYVPSVVRSFFPFNGVTNYEGTSKSLLAVQVTELADGIFIGCNFNHVVADGQSTWHFINSWAEMSRGCHHQISKPPTLERWFPNGIQQPIRFPLTIEPQKNYSEELSFSSSDDEKFYLSERIFHFTKKKIMQLKSKVNAAIDTKKISSLQALLTHLWCSVIRSKHLDPQEDALNTFIIGVRPRLVPPLPEDYFGNAIIGCGFKMKAGELLEEGGIGKGALLMNKLIALHSNEKLKNDYESWLRNPSFIRLDSLSTKNFLAISSSPWFDVYGNDFGWGKPVAVRSGNRTNGMVCAFAGIEEGSIDLQVCLPYKILEAMGNDHEFMDMVN