AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50420


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50420.t1 MTR_4g022290 86.000 450 56 2 1 450 1 443 0.0 785
MS.gene50420.t1 MTR_4g094828 80.786 458 68 4 1 449 1 447 0.0 749
MS.gene50420.t1 MTR_8g079710 74.009 454 103 8 1 450 1 443 0.0 660
MS.gene50420.t1 MTR_4g094835 53.392 457 189 10 1 448 1 442 3.69e-166 476
MS.gene50420.t1 MTR_4g094842 51.948 462 184 8 1 445 1 441 1.17e-159 459
MS.gene50420.t1 MTR_0190s0020 49.563 458 203 7 1 449 1 439 4.45e-153 442
MS.gene50420.t1 MTR_4g094838 50.221 452 186 9 4 444 1 424 2.07e-146 425
MS.gene50420.t1 MTR_4g022800 49.679 467 189 11 1 449 1 439 2.57e-146 425
MS.gene50420.t1 MTR_4g022670 49.142 466 192 11 1 449 1 438 6.92e-146 424
MS.gene50420.t1 MTR_3g026310 49.269 479 189 13 1 455 1 449 8.65e-146 424
MS.gene50420.t1 MTR_4g022825 49.892 465 189 12 1 449 1 437 1.98e-145 422
MS.gene50420.t1 MTR_4g022720 49.783 460 192 11 1 449 1 432 1.10e-144 421
MS.gene50420.t1 MTR_8g041770 49.345 458 203 10 1 449 1 438 2.68e-144 420
MS.gene50420.t1 MTR_7g026860 49.032 465 191 11 1 449 1 435 7.15e-143 416
MS.gene50420.t1 MTR_3g088420 49.241 461 191 12 1 449 1 430 1.78e-142 415
MS.gene50420.t1 MTR_4g094832 55.236 382 143 8 75 444 57 422 4.77e-139 406
MS.gene50420.t1 MTR_4g022760 43.871 465 174 10 1 449 1 394 2.23e-118 352
MS.gene50420.t1 MTR_6g016815 40.570 456 174 11 1 450 15 379 1.42e-99 303
MS.gene50420.t1 MTR_3g101900 35.227 440 247 11 30 454 29 445 4.04e-80 256
MS.gene50420.t1 MTR_3g101930 36.062 452 240 14 27 454 26 452 2.84e-79 253
MS.gene50420.t1 MTR_3g102010 36.000 450 250 13 27 457 26 456 1.73e-77 249
MS.gene50420.t1 MTR_3g101960 35.909 440 242 13 25 444 23 442 1.75e-76 246
MS.gene50420.t1 MTR_3g102020 35.714 448 247 14 25 451 23 450 6.13e-76 245
MS.gene50420.t1 MTR_3g101970 35.202 446 236 14 25 450 23 435 6.91e-76 244
MS.gene50420.t1 MTR_8g040760 54.338 219 90 4 1 214 1 214 2.72e-74 233
MS.gene50420.t1 MTR_5g081960 33.056 481 249 13 29 456 25 485 1.41e-68 226
MS.gene50420.t1 MTR_3g070170 32.088 455 263 11 21 444 23 462 9.99e-63 211
MS.gene50420.t1 MTR_4g015180 33.827 473 248 17 24 444 20 479 2.69e-62 210
MS.gene50420.t1 MTR_4g094825 56.150 187 74 5 24 204 85 269 1.07e-61 206
MS.gene50420.t1 MTR_4g094825 47.945 146 62 4 300 444 251 383 9.11e-33 128
MS.gene50420.t1 MTR_7g026850 61.146 157 58 3 30 185 7 161 1.73e-60 196
MS.gene50420.t1 MTR_6g034495 66.379 116 37 1 72 185 45 160 1.82e-46 159
MS.gene50420.t1 MTR_8g040780 40.664 241 123 5 208 445 2 225 2.24e-46 161
MS.gene50420.t1 MTR_3g102030 30.925 346 185 12 72 405 17 320 6.49e-41 149
MS.gene50420.t1 MTR_6g045553 28.821 458 265 13 27 444 31 467 1.53e-37 143
MS.gene50420.t1 MTR_7g026770 42.553 188 78 4 148 335 132 289 3.31e-36 135
MS.gene50420.t1 MTR_7g026830 70.732 82 24 0 91 172 16 97 3.96e-35 126
MS.gene50420.t1 MTR_6g034485 41.135 141 63 3 193 329 1 125 1.85e-27 106
MS.gene50420.t1 MTR_8g075610 26.055 403 244 16 61 443 47 415 3.77e-27 113
MS.gene50420.t1 MTR_2g105330 25.592 422 255 18 48 443 39 427 5.17e-25 107
MS.gene50420.t1 MTR_2g064495 25.592 422 255 18 48 443 39 427 7.27e-25 106
MS.gene50420.t1 MTR_4g007540 25.476 420 259 17 48 443 39 428 2.27e-24 105
MS.gene50420.t1 MTR_5g026320 25.678 479 289 20 3 453 17 456 4.48e-24 104
MS.gene50420.t1 MTR_1g040175 24.586 423 266 17 36 441 46 432 7.33e-21 95.1
MS.gene50420.t1 MTR_3g049150 24.186 430 252 16 48 437 38 433 7.37e-21 95.1
MS.gene50420.t1 MTR_7g027175 23.325 403 272 13 51 443 58 433 9.41e-21 94.7
MS.gene50420.t1 MTR_4g097090 23.884 448 259 18 31 433 30 440 2.32e-20 93.6
MS.gene50420.t1 MTR_8g096530 25.490 357 216 14 61 400 59 382 7.98e-20 92.0
MS.gene50420.t1 MTR_3g026950 25.170 441 259 19 37 437 28 437 1.39e-19 91.3
MS.gene50420.t1 MTR_2g089765 24.537 432 234 19 28 400 36 434 1.89e-19 91.3
MS.gene50420.t1 MTR_7g085930 25.058 431 267 15 51 444 49 460 2.62e-19 90.5
MS.gene50420.t1 MTR_1g040195 24.887 442 268 17 32 455 23 418 3.73e-19 89.7
MS.gene50420.t1 MTR_6g015815 23.478 460 262 17 31 441 34 452 9.12e-18 85.9
MS.gene50420.t1 MTR_1g081660 24.460 417 230 17 31 408 31 401 1.52e-17 85.1
MS.gene50420.t1 MTR_6g015830 25.672 409 241 17 31 403 34 415 2.02e-17 84.7
MS.gene50420.t1 MTR_3g098980 22.139 402 257 13 61 443 66 430 3.00e-17 84.0
MS.gene50420.t1 MTR_7g014200 23.297 455 281 16 29 439 28 458 3.39e-17 84.3
MS.gene50420.t1 MTR_3g028210 23.326 463 292 15 31 458 31 465 1.60e-16 82.0
MS.gene50420.t1 MTR_3g087640 23.503 451 279 18 36 439 31 462 3.96e-16 80.9
MS.gene50420.t1 MTR_7g114470 23.409 440 265 21 32 434 25 429 4.21e-16 80.9
MS.gene50420.t1 MTR_4g127650 23.114 411 242 18 61 433 66 440 4.25e-16 80.9
MS.gene50420.t1 MTR_7g014330 23.632 402 265 15 31 404 26 413 5.43e-16 80.5
MS.gene50420.t1 MTR_4g013050 23.457 405 219 16 59 433 51 394 1.73e-15 78.6
MS.gene50420.t1 MTR_8g012750 24.703 421 248 17 28 400 23 422 1.85e-15 79.0
MS.gene50420.t1 MTR_5g084870 24.834 451 271 19 3 430 2 407 2.17e-15 78.6
MS.gene50420.t1 MTR_6g015320 24.439 401 255 17 31 403 29 409 2.47e-15 78.2
MS.gene50420.t1 MTR_5g090880 24.282 383 215 14 61 402 67 415 3.95e-15 77.8
MS.gene50420.t1 MTR_7g014250 23.267 404 256 16 35 404 30 413 1.05e-14 76.3
MS.gene50420.t1 MTR_3g086020 25.822 426 243 19 30 431 22 398 1.23e-14 75.9
MS.gene50420.t1 MTR_6g015850 24.272 412 240 14 31 403 34 412 1.67e-14 75.9
MS.gene50420.t1 MTR_1g040320 24.155 414 235 17 56 455 59 407 3.46e-14 74.7
MS.gene50420.t1 MTR_2g089755 24.939 409 258 17 31 403 37 432 6.95e-14 73.9
MS.gene50420.t1 MTR_5g090810 22.927 410 236 13 39 401 36 412 9.10e-14 73.6
MS.gene50420.t1 MTR_3g112180 26.000 300 181 11 35 318 38 312 1.68e-13 72.8
MS.gene50420.t1 MTR_7g014610 22.319 457 281 17 34 439 32 465 1.99e-13 72.4
MS.gene50420.t1 MTR_8g079980 22.581 372 226 13 54 403 52 383 4.44e-13 71.2
MS.gene50420.t1 MTR_6g053810 25.989 354 221 15 61 396 71 401 1.12e-12 70.1
MS.gene50420.t1 MTR_4g055170 25.256 293 185 10 61 340 69 340 1.83e-12 69.3
MS.gene50420.t1 MTR_5g090830 22.683 410 237 14 39 401 36 412 2.14e-12 69.3
MS.gene50420.t1 MTR_7g114560 28.962 183 105 8 32 198 24 197 7.51e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50420.t1 AT5G67150 37.802 455 250 12 3 444 4 438 6.05e-95 294
MS.gene50420.t1 AT3G50300 37.660 470 248 14 1 450 1 445 4.26e-88 276
MS.gene50420.t1 AT3G50270 35.903 454 259 10 4 444 6 440 4.31e-84 266
MS.gene50420.t1 AT5G67160 37.089 426 234 10 27 444 25 424 6.26e-82 260
MS.gene50420.t1 AT5G38130 36.508 441 253 11 1 433 21 442 1.18e-80 257
MS.gene50420.t1 AT3G50280 34.573 457 264 11 1 444 1 435 2.96e-80 256
MS.gene50420.t1 AT5G42830 35.648 432 242 13 30 444 29 441 4.45e-76 245
MS.gene50420.t1 AT5G07850 35.023 434 245 11 30 444 30 445 1.63e-72 236
MS.gene50420.t1 AT5G07860 35.566 433 240 10 30 444 30 441 5.41e-72 234
MS.gene50420.t1 AT2G39980 32.983 476 265 12 11 444 9 472 2.44e-69 228
MS.gene50420.t1 AT5G01210 33.261 460 258 11 24 448 20 465 4.65e-69 228
MS.gene50420.t1 AT5G07870 35.177 452 244 15 30 451 30 462 5.77e-64 214
MS.gene50420.t1 AT3G50280 34.891 321 185 7 132 444 1 305 1.88e-53 182
MS.gene50420.t1 AT5G23940 30.926 443 256 12 27 441 21 441 7.56e-49 174
MS.gene50420.t1 AT3G50290 38.380 284 149 7 126 398 1 269 7.90e-49 168
MS.gene50420.t1 AT5G41040 27.000 400 249 15 58 443 66 436 1.47e-25 108
MS.gene50420.t1 AT5G41040 27.000 400 249 15 58 443 82 452 1.57e-25 108
MS.gene50420.t1 AT5G48930 25.547 411 250 17 48 433 39 418 2.66e-22 99.4
MS.gene50420.t1 AT3G48720 26.066 422 261 17 41 443 37 426 9.32e-22 97.8
MS.gene50420.t1 AT5G63560 24.936 393 236 15 61 434 61 413 2.36e-20 93.6
MS.gene50420.t1 AT5G57840 25.476 420 243 18 48 433 39 422 5.62e-19 89.4
MS.gene50420.t1 AT2G19070 25.902 471 261 21 25 442 9 444 2.48e-18 87.4
MS.gene50420.t1 AT3G29590 23.007 439 280 15 31 439 28 438 5.00e-18 86.7
MS.gene50420.t1 AT1G78990 25.867 375 221 14 50 401 56 396 1.69e-17 85.1
MS.gene50420.t1 AT3G26040 27.679 448 245 21 30 444 20 421 1.77e-17 84.7
MS.gene50420.t1 AT3G03480 25.227 440 264 18 32 439 33 439 1.79e-17 85.1
MS.gene50420.t1 AT5G17540 23.971 413 236 16 32 408 23 393 5.63e-17 83.6
MS.gene50420.t1 AT1G32910 24.661 369 216 13 61 401 65 399 1.78e-16 82.0
MS.gene50420.t1 AT5G07080 23.775 408 242 15 61 438 70 438 1.21e-15 79.3
MS.gene50420.t1 AT5G47950 23.618 398 247 14 57 442 57 409 9.72e-15 76.3
MS.gene50420.t1 AT5G39080 24.374 439 275 16 31 433 28 445 1.55e-14 75.9
MS.gene50420.t1 AT5G16410 23.499 383 224 13 48 401 77 419 1.97e-14 75.9
MS.gene50420.t1 AT5G23970 23.877 423 250 17 47 444 38 413 8.09e-14 73.6
MS.gene50420.t1 AT1G24420 25.581 344 206 14 72 403 76 381 3.68e-13 71.6
MS.gene50420.t1 AT1G24420 25.581 344 206 14 72 403 76 381 3.68e-13 71.6
MS.gene50420.t1 AT2G40230 24.259 371 229 14 53 403 51 389 1.15e-11 67.0
MS.gene50420.t1 AT3G47170 21.711 304 194 8 61 340 70 353 1.31e-11 67.0
MS.gene50420.t1 AT1G31490 24.377 361 221 13 58 401 59 384 3.60e-11 65.5
MS.gene50420.t1 AT1G27620 23.514 387 269 15 61 442 58 422 4.63e-11 65.1
MS.gene50420.t1 AT5G47980 23.544 412 245 18 60 444 60 428 7.37e-11 64.3

Find 94 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTTAGAAGAGAATTATTT+TGG 0.198109 4.4:+79463632 MS.gene50420:CDS
TTGTCTTCCTTATGATATTT+TGG 0.223517 4.4:+79464003 MS.gene50420:CDS
CATTCTTGTCCTACAATCTA+TGG 0.234125 4.4:-79463603 None:intergenic
GGATCTCAAATTTCTCAAAT+TGG 0.239669 4.4:+79462812 MS.gene50420:CDS
GGATATGTAATAATGCAAAA+AGG 0.251454 4.4:+79463674 MS.gene50420:CDS
ATGGGAAGGTTAACATTGTT+TGG 0.295352 4.4:-79463311 None:intergenic
TATGGAAAAGAAGACCTCTT+TGG 0.321294 4.4:-79462842 None:intergenic
CATCACCATGATTTGTTGCT+TGG 0.340899 4.4:-79462746 None:intergenic
TTTGTTTAAGATGGTCAATT+TGG 0.343039 4.4:-79462878 None:intergenic
AAATAATTCTCTTCTAACTT+TGG 0.349532 4.4:-79463629 None:intergenic
GTGTAGACGGCAAGTCATTT+TGG 0.349934 4.4:+79463210 MS.gene50420:CDS
ATGGAAAAGAAGACCTCTTT+GGG 0.360598 4.4:-79462841 None:intergenic
ACGAATGCAGACCTTTCTTC+TGG 0.360777 4.4:-79463575 None:intergenic
GTTGAAAACCCCGTTTATAT+CGG 0.362533 4.4:+79463799 MS.gene50420:CDS
TTTGGCATTTCATCAACTCT+TGG 0.368556 4.4:+79463228 MS.gene50420:CDS
TTACGGTAATGACTTTGGTT+GGG 0.373170 4.4:+79463880 MS.gene50420:CDS
TTGGCATTTCATCAACTCTT+GGG 0.376104 4.4:+79463229 MS.gene50420:CDS
AGCCTATTGCAGTTAGAAAT+GGG 0.388093 4.4:+79463906 MS.gene50420:CDS
GTTGGACTGCAAAGAAATAT+TGG 0.392396 4.4:+79463929 MS.gene50420:CDS
ACCCCATTTCTAACTGCAAT+AGG 0.394232 4.4:-79463908 None:intergenic
TCGTCTTACCCATAGATTGT+AGG 0.400779 4.4:+79463594 MS.gene50420:CDS
AAGCCTATTGCAGTTAGAAA+TGG 0.401535 4.4:+79463905 MS.gene50420:CDS
AACTATTACGGTAATGACTT+TGG 0.405935 4.4:+79463875 MS.gene50420:CDS
TTAATCGACGGTATCTTCAT+TGG 0.408333 4.4:+79463167 MS.gene50420:CDS
CTACCTGGCTCCGATATAAA+CGG 0.425817 4.4:-79463809 None:intergenic
TACCTGGCTCCGATATAAAC+GGG 0.427106 4.4:-79463808 None:intergenic
GGGAGTGGAATCGGAGCAAC+TGG 0.428061 4.4:+79463653 MS.gene50420:CDS
TCGCCACGGTTCAACTATTA+CGG 0.439762 4.4:+79463863 MS.gene50420:CDS
AAATTGTTCATTTATCTCCA+TGG 0.445544 4.4:+79462790 MS.gene50420:CDS
ATTACGGTAATGACTTTGGT+TGG 0.447960 4.4:+79463879 MS.gene50420:CDS
AGGCAAGTGTTTGTTTAAGA+TGG 0.458293 4.4:-79462887 None:intergenic
TACGGTAATGACTTTGGTTG+GGG 0.461686 4.4:+79463881 MS.gene50420:CDS
GTGAATGGTGGGGCAGAAGA+AGG 0.474870 4.4:+79463962 MS.gene50420:CDS
ATAGCCTCCAAAATATCATA+AGG 0.481866 4.4:-79464010 None:intergenic
AATTGTTCATTTATCTCCAT+GGG 0.481963 4.4:+79462791 MS.gene50420:CDS
AAAGGGGAATTAATGGAAAG+TGG 0.488381 4.4:+79463692 MS.gene50420:CDS
GGAAACCAACGTTCGAAAGA+AGG 0.490929 4.4:-79463290 None:intergenic
ACAATCCAAGCAACAAATCA+TGG 0.492574 4.4:+79462741 MS.gene50420:CDS
TAAGAATAATGAAGAAACAA+TGG 0.493631 4.4:-79463081 None:intergenic
ACAATGGGAGGAACATACTT+TGG 0.495798 4.4:-79463065 None:intergenic
AAGAATAATGAAGAAACAAT+GGG 0.496513 4.4:-79463080 None:intergenic
TTGAGAAATTTGAGATCCCA+TGG 0.499411 4.4:-79462807 None:intergenic
GGTAGAGTTATTGTGAATGG+TGG 0.501616 4.4:+79463950 MS.gene50420:CDS
ACCAGCGTTGTTACAAATGA+TGG 0.505482 4.4:-79462988 None:intergenic
GAATTTGTTCCTTTGTAAAG+TGG 0.513443 4.4:-79463397 None:intergenic
ATTGGTAGAGTTATTGTGAA+TGG 0.514289 4.4:+79463947 MS.gene50420:CDS
AACTTCTTTCCACCACTCGC+TGG 0.516081 4.4:+79462918 MS.gene50420:CDS
AACTAGAATCCATGGACATA+GGG 0.518441 4.4:-79464042 None:intergenic
TAATGAGTGATATTGAATCT+TGG 0.523510 4.4:+79463777 MS.gene50420:CDS
TGGGATTCGTAAAATTGCTA+TGG 0.525481 4.4:+79463712 MS.gene50420:CDS
TACTTATAATTAAACGCCAA+AGG 0.529613 4.4:-79463538 None:intergenic
AAGGGGAATTAATGGAAAGT+GGG 0.533012 4.4:+79463693 MS.gene50420:CDS
ATGGAACATGAGGCGTTGCT+AGG 0.533394 4.4:-79462969 None:intergenic
AAACTAGAATCCATGGACAT+AGG 0.534361 4.4:-79464043 None:intergenic
AAAATCTTAAACCAGAAGAA+AGG 0.538946 4.4:+79463564 MS.gene50420:CDS
CATACGGTTTCCTTTCACAA+AGG 0.546568 4.4:+79463331 MS.gene50420:CDS
ACCCCGTTTATATCGGAGCC+AGG 0.547815 4.4:+79463806 MS.gene50420:CDS
GTTGATGAAACTAGAATCCA+TGG 0.549745 4.4:-79464050 None:intergenic
AGATTACTTGATGATCTACC+TGG 0.550288 4.4:-79463824 None:intergenic
ACAATGTTAACCTTCCCATA+CGG 0.562792 4.4:+79463315 MS.gene50420:CDS
CTTTGTGAAAGGAAACCGTA+TGG 0.565930 4.4:-79463330 None:intergenic
TGGTTGAAGTATGTCGGCAA+TGG 0.566982 4.4:-79463045 None:intergenic
ATTGCAGTTAGAAATGGGGT+TGG 0.568648 4.4:+79463911 MS.gene50420:CDS
ATACTTTGGTTGAAGTATGT+CGG 0.572459 4.4:-79463051 None:intergenic
AAAAGAAGACCTCTTTGGGT+TGG 0.572933 4.4:-79462837 None:intergenic
AGAAGACGACCCTATGTCCA+TGG 0.573614 4.4:+79464033 MS.gene50420:CDS
CAAGCAACAAATCATGGTGA+TGG 0.574475 4.4:+79462747 MS.gene50420:CDS
ATTCTTGTCCTACAATCTAT+GGG 0.578627 4.4:-79463602 None:intergenic
TGAAAGGAAACCGTATGGGA+AGG 0.580544 4.4:-79463325 None:intergenic
GTAGAGTTATTGTGAATGGT+GGG 0.583851 4.4:+79463951 MS.gene50420:CDS
TGGTGGAAAGAAGTTAAGTG+TGG 0.592664 4.4:-79462910 None:intergenic
GTTACTTGTACTGCAAGTAG+TGG 0.602193 4.4:-79463140 None:intergenic
TCGAATCTTCCACTTTACAA+AGG 0.603894 4.4:+79463388 MS.gene50420:CDS
AAGTACCTTCTTTCGAACGT+TGG 0.604822 4.4:+79463285 MS.gene50420:CDS
ATTAACCATGTGTGTGTAGA+CGG 0.608212 4.4:+79463197 MS.gene50420:CDS
GCCTATTGCAGTTAGAAATG+GGG 0.609427 4.4:+79463907 MS.gene50420:CDS
TCCATCATTTGTAACAACGC+TGG 0.618527 4.4:+79462987 MS.gene50420:CDS
ACAAATCATGGTGATGGTGA+TGG 0.619825 4.4:+79462753 MS.gene50420:CDS
TGATAGCAAGCAGTTCGCCA+CGG 0.626185 4.4:+79463849 MS.gene50420:CDS
ACAAGACGACCAGCGAGTGG+TGG 0.630223 4.4:-79462927 None:intergenic
GTTACAAATGATGGAACATG+AGG 0.636643 4.4:-79462979 None:intergenic
AATTGCTATGGAAATGAACA+AGG 0.638167 4.4:+79463724 MS.gene50420:CDS
AAATTGGCACCAACCCAAAG+AGG 0.639346 4.4:+79462828 MS.gene50420:CDS
TTTGTGAAAGGAAACCGTAT+GGG 0.642383 4.4:-79463329 None:intergenic
TCTTGGGCTGAAATCTCACA+AGG 0.661149 4.4:+79463245 MS.gene50420:CDS
TGGAAAGAAGTTAAGTGTGG+AGG 0.665000 4.4:-79462907 None:intergenic
CAAGTAACAGAGTTAATCGA+CGG 0.676652 4.4:+79463155 MS.gene50420:CDS
AATAATGAAGAAACAATGGG+AGG 0.681867 4.4:-79463077 None:intergenic
ACTTGCCGTCTACACACACA+TGG 0.683354 4.4:-79463202 None:intergenic
ATTACAAGACGACCAGCGAG+TGG 0.695572 4.4:-79462930 None:intergenic
AATGATGTGAAAAGCTACGA+AGG 0.699069 4.4:+79463107 MS.gene50420:CDS
TAGAGTTATTGTGAATGGTG+GGG 0.705324 4.4:+79463952 MS.gene50420:CDS
ACCTGGCTCCGATATAAACG+GGG 0.710991 4.4:-79463807 None:intergenic
TTACCGTAATAGTTGAACCG+TGG 0.733929 4.4:-79463866 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTCTCTTCTAACTT+TGG - chr4.4:79463632-79463651 None:intergenic 20.0%
!! AAGAATAATGAAGAAACAAT+GGG - chr4.4:79463083-79463102 None:intergenic 20.0%
!! ATATGTAATAATGCAAAAAG+GGG + chr4.4:79463676-79463695 MS.gene50420:CDS 20.0%
!! GATATGTAATAATGCAAAAA+GGG + chr4.4:79463675-79463694 MS.gene50420:CDS 20.0%
!! TAAGAATAATGAAGAAACAA+TGG - chr4.4:79463084-79463103 None:intergenic 20.0%
!!! AAGTTAGAAGAGAATTATTT+TGG + chr4.4:79463632-79463651 MS.gene50420:CDS 20.0%
!!! AGTTAGAAGAGAATTATTTT+GGG + chr4.4:79463633-79463652 MS.gene50420:CDS 20.0%
! AAAATCTTAAACCAGAAGAA+AGG + chr4.4:79463564-79463583 MS.gene50420:CDS 25.0%
! AAATTGTTCATTTATCTCCA+TGG + chr4.4:79462790-79462809 MS.gene50420:CDS 25.0%
! AATTGTTCATTTATCTCCAT+GGG + chr4.4:79462791-79462810 MS.gene50420:CDS 25.0%
! GGATATGTAATAATGCAAAA+AGG + chr4.4:79463674-79463693 MS.gene50420:CDS 25.0%
! TAATGAGTGATATTGAATCT+TGG + chr4.4:79463777-79463796 MS.gene50420:CDS 25.0%
! TACTTATAATTAAACGCCAA+AGG - chr4.4:79463541-79463560 None:intergenic 25.0%
!! TTGTCTTCCTTATGATATTT+TGG + chr4.4:79464003-79464022 MS.gene50420:CDS 25.0%
!! TTTGTTTAAGATGGTCAATT+TGG - chr4.4:79462881-79462900 None:intergenic 25.0%
!!! TTTGGTTTGTTTTAGGTTTA+TGG - chr4.4:79462863-79462882 None:intergenic 25.0%
AACTATTACGGTAATGACTT+TGG + chr4.4:79463875-79463894 MS.gene50420:CDS 30.0%
AATAATGAAGAAACAATGGG+AGG - chr4.4:79463080-79463099 None:intergenic 30.0%
AATGCAAAAAGGGGAATTAA+TGG + chr4.4:79463685-79463704 MS.gene50420:CDS 30.0%
AATTGCTATGGAAATGAACA+AGG + chr4.4:79463724-79463743 MS.gene50420:CDS 30.0%
ATACTTTGGTTGAAGTATGT+CGG - chr4.4:79463054-79463073 None:intergenic 30.0%
ATAGCCTCCAAAATATCATA+AGG - chr4.4:79464013-79464032 None:intergenic 30.0%
ATTCTTGTCCTACAATCTAT+GGG - chr4.4:79463605-79463624 None:intergenic 30.0%
ATTGGTAGAGTTATTGTGAA+TGG + chr4.4:79463947-79463966 MS.gene50420:CDS 30.0%
GGATCTCAAATTTCTCAAAT+TGG + chr4.4:79462812-79462831 MS.gene50420:CDS 30.0%
! GAATTTGTTCCTTTGTAAAG+TGG - chr4.4:79463400-79463419 None:intergenic 30.0%
!! TCTTCCTTATGATATTTTGG+AGG + chr4.4:79464006-79464025 MS.gene50420:CDS 30.0%
!!! AATTATTTTGGGAGTGGAAT+CGG + chr4.4:79463644-79463663 MS.gene50420:CDS 30.0%
!!! TGGTCAATTTGGTTTGTTTT+AGG - chr4.4:79462870-79462889 None:intergenic 30.0%
AAACTAGAATCCATGGACAT+AGG - chr4.4:79464046-79464065 None:intergenic 35.0%
AAAGGGGAATTAATGGAAAG+TGG + chr4.4:79463692-79463711 MS.gene50420:CDS 35.0%
AACTAGAATCCATGGACATA+GGG - chr4.4:79464045-79464064 None:intergenic 35.0%
AAGCCTATTGCAGTTAGAAA+TGG + chr4.4:79463905-79463924 MS.gene50420:CDS 35.0%
AAGGGGAATTAATGGAAAGT+GGG + chr4.4:79463693-79463712 MS.gene50420:CDS 35.0%
AATGATGTGAAAAGCTACGA+AGG + chr4.4:79463107-79463126 MS.gene50420:CDS 35.0%
ACAATCCAAGCAACAAATCA+TGG + chr4.4:79462741-79462760 MS.gene50420:CDS 35.0%
ACAATGTTAACCTTCCCATA+CGG + chr4.4:79463315-79463334 MS.gene50420:CDS 35.0%
AGCCTATTGCAGTTAGAAAT+GGG + chr4.4:79463906-79463925 MS.gene50420:CDS 35.0%
AGGCAAGTGTTTGTTTAAGA+TGG - chr4.4:79462890-79462909 None:intergenic 35.0%
ATGGAAAAGAAGACCTCTTT+GGG - chr4.4:79462844-79462863 None:intergenic 35.0%
ATGGGAAGGTTAACATTGTT+TGG - chr4.4:79463314-79463333 None:intergenic 35.0%
ATTAACCATGTGTGTGTAGA+CGG + chr4.4:79463197-79463216 MS.gene50420:CDS 35.0%
ATTACGGTAATGACTTTGGT+TGG + chr4.4:79463879-79463898 MS.gene50420:CDS 35.0%
CAAGTAACAGAGTTAATCGA+CGG + chr4.4:79463155-79463174 MS.gene50420:CDS 35.0%
CATTCTTGTCCTACAATCTA+TGG - chr4.4:79463606-79463625 None:intergenic 35.0%
GTAGAGTTATTGTGAATGGT+GGG + chr4.4:79463951-79463970 MS.gene50420:CDS 35.0%
GTTGAAAACCCCGTTTATAT+CGG + chr4.4:79463799-79463818 MS.gene50420:CDS 35.0%
GTTGGACTGCAAAGAAATAT+TGG + chr4.4:79463929-79463948 MS.gene50420:CDS 35.0%
TAGAGTTATTGTGAATGGTG+GGG + chr4.4:79463952-79463971 MS.gene50420:CDS 35.0%
TATGGAAAAGAAGACCTCTT+TGG - chr4.4:79462845-79462864 None:intergenic 35.0%
TCGAATCTTCCACTTTACAA+AGG + chr4.4:79463388-79463407 MS.gene50420:CDS 35.0%
TGGGATTCGTAAAATTGCTA+TGG + chr4.4:79463712-79463731 MS.gene50420:CDS 35.0%
TTAATCGACGGTATCTTCAT+TGG + chr4.4:79463167-79463186 MS.gene50420:CDS 35.0%
TTACGGTAATGACTTTGGTT+GGG + chr4.4:79463880-79463899 MS.gene50420:CDS 35.0%
TTGGCATTTCATCAACTCTT+GGG + chr4.4:79463229-79463248 MS.gene50420:CDS 35.0%
TTTGGCATTTCATCAACTCT+TGG + chr4.4:79463228-79463247 MS.gene50420:CDS 35.0%
TTTGTGAAAGGAAACCGTAT+GGG - chr4.4:79463332-79463351 None:intergenic 35.0%
! AGATTACTTGATGATCTACC+TGG - chr4.4:79463827-79463846 None:intergenic 35.0%
! GTTACAAATGATGGAACATG+AGG - chr4.4:79462982-79463001 None:intergenic 35.0%
! GTTGATGAAACTAGAATCCA+TGG - chr4.4:79464053-79464072 None:intergenic 35.0%
! TTGAGAAATTTGAGATCCCA+TGG - chr4.4:79462810-79462829 None:intergenic 35.0%
! TTTTGTGCATCCTTTGTGAA+AGG - chr4.4:79463344-79463363 None:intergenic 35.0%
!!! AGGTACTTTTGTTGGCTTAT+TGG - chr4.4:79463273-79463292 None:intergenic 35.0%
!!! GAAGAGAATTATTTTGGGAG+TGG + chr4.4:79463638-79463657 MS.gene50420:CDS 35.0%
!!! TCGAAAGAAGGTACTTTTGT+TGG - chr4.4:79463281-79463300 None:intergenic 35.0%
AAAAGAAGACCTCTTTGGGT+TGG - chr4.4:79462840-79462859 None:intergenic 40.0%
AAGTACCTTCTTTCGAACGT+TGG + chr4.4:79463285-79463304 MS.gene50420:CDS 40.0%
ACAATGGGAGGAACATACTT+TGG - chr4.4:79463068-79463087 None:intergenic 40.0%
ACCAGCGTTGTTACAAATGA+TGG - chr4.4:79462991-79463010 None:intergenic 40.0%
ACCCCATTTCTAACTGCAAT+AGG - chr4.4:79463911-79463930 None:intergenic 40.0%
ATTGCAGTTAGAAATGGGGT+TGG + chr4.4:79463911-79463930 MS.gene50420:CDS 40.0%
CAAGCAACAAATCATGGTGA+TGG + chr4.4:79462747-79462766 MS.gene50420:CDS 40.0%
CATACGGTTTCCTTTCACAA+AGG + chr4.4:79463331-79463350 MS.gene50420:CDS 40.0%
CTTTGTGAAAGGAAACCGTA+TGG - chr4.4:79463333-79463352 None:intergenic 40.0%
GCCTATTGCAGTTAGAAATG+GGG + chr4.4:79463907-79463926 MS.gene50420:CDS 40.0%
GGTAGAGTTATTGTGAATGG+TGG + chr4.4:79463950-79463969 MS.gene50420:CDS 40.0%
GTTACTTGTACTGCAAGTAG+TGG - chr4.4:79463143-79463162 None:intergenic 40.0%
TACGGTAATGACTTTGGTTG+GGG + chr4.4:79463881-79463900 MS.gene50420:CDS 40.0%
TCCATCATTTGTAACAACGC+TGG + chr4.4:79462987-79463006 MS.gene50420:CDS 40.0%
TCGTCTTACCCATAGATTGT+AGG + chr4.4:79463594-79463613 MS.gene50420:CDS 40.0%
TGGAAAGAAGTTAAGTGTGG+AGG - chr4.4:79462910-79462929 None:intergenic 40.0%
TGGTGGAAAGAAGTTAAGTG+TGG - chr4.4:79462913-79462932 None:intergenic 40.0%
TTACCGTAATAGTTGAACCG+TGG - chr4.4:79463869-79463888 None:intergenic 40.0%
!! AAGCGCTTTTAAGTCACCTT+TGG + chr4.4:79463522-79463541 MS.gene50420:CDS 40.0%
!! AATGGTAGTGTTTTCTGCTG+TGG - chr4.4:79463030-79463049 None:intergenic 40.0%
!! ACAAATCATGGTGATGGTGA+TGG + chr4.4:79462753-79462772 MS.gene50420:CDS 40.0%
!! CATCACCATGATTTGTTGCT+TGG - chr4.4:79462749-79462768 None:intergenic 40.0%
AAATTGGCACCAACCCAAAG+AGG + chr4.4:79462828-79462847 MS.gene50420:CDS 45.0%
ACGAATGCAGACCTTTCTTC+TGG - chr4.4:79463578-79463597 None:intergenic 45.0%
CTACCTGGCTCCGATATAAA+CGG - chr4.4:79463812-79463831 None:intergenic 45.0%
GGAAACCAACGTTCGAAAGA+AGG - chr4.4:79463293-79463312 None:intergenic 45.0%
TACCTGGCTCCGATATAAAC+GGG - chr4.4:79463811-79463830 None:intergenic 45.0%
TCGCCACGGTTCAACTATTA+CGG + chr4.4:79463863-79463882 MS.gene50420:CDS 45.0%
TCTTGGGCTGAAATCTCACA+AGG + chr4.4:79463245-79463264 MS.gene50420:CDS 45.0%
TGAAAGGAAACCGTATGGGA+AGG - chr4.4:79463328-79463347 None:intergenic 45.0%
TGGTTGAAGTATGTCGGCAA+TGG - chr4.4:79463048-79463067 None:intergenic 45.0%
! GTGTAGACGGCAAGTCATTT+TGG + chr4.4:79463210-79463229 MS.gene50420:CDS 45.0%
AACTTCTTTCCACCACTCGC+TGG + chr4.4:79462918-79462937 MS.gene50420:CDS 50.0%
ACCTGGCTCCGATATAAACG+GGG - chr4.4:79463810-79463829 None:intergenic 50.0%
ACTTGCCGTCTACACACACA+TGG - chr4.4:79463205-79463224 None:intergenic 50.0%
AGAAGACGACCCTATGTCCA+TGG + chr4.4:79464033-79464052 MS.gene50420:CDS 50.0%
ATTACAAGACGACCAGCGAG+TGG - chr4.4:79462933-79462952 None:intergenic 50.0%
TGATAGCAAGCAGTTCGCCA+CGG + chr4.4:79463849-79463868 MS.gene50420:CDS 50.0%
! TAGTGTTTTCTGCTGTGGCG+TGG - chr4.4:79463025-79463044 None:intergenic 50.0%
!! ATGGAACATGAGGCGTTGCT+AGG - chr4.4:79462972-79462991 None:intergenic 50.0%
ACCCCGTTTATATCGGAGCC+AGG + chr4.4:79463806-79463825 MS.gene50420:CDS 55.0%
! GTGAATGGTGGGGCAGAAGA+AGG + chr4.4:79463962-79463981 MS.gene50420:CDS 55.0%
ACAAGACGACCAGCGAGTGG+TGG - chr4.4:79462930-79462949 None:intergenic 60.0%
GGGAGTGGAATCGGAGCAAC+TGG + chr4.4:79463653-79463672 MS.gene50420:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 79462711 79464096 79462711 ID=MS.gene50420
chr4.4 mRNA 79462711 79464096 79462711 ID=MS.gene50420.t1;Parent=MS.gene50420
chr4.4 exon 79462711 79464096 79462711 ID=MS.gene50420.t1.exon1;Parent=MS.gene50420.t1
chr4.4 CDS 79462711 79464096 79462711 ID=cds.MS.gene50420.t1;Parent=MS.gene50420.t1
Gene Sequence

>MS.gene50420

ATGACAGAGATTCAACTTATCTCAACTTCTACAATCCAAGCAACAAATCATGGTGATGGTGATGGCAACTCAACTCACAAAATTGTTCATTTATCTCCATGGGATCTCAAATTTCTCAAATTGGCACCAACCCAAAGAGGTCTTCTTTTCCATAAACCTAAAACAAACCAAATTGACCATCTTAAACAAACACTTGCCTCCACACTTAACTTCTTTCCACCACTCGCTGGTCGTCTTGTAATAACACAACACGACGAACCTAGCAACGCCTCATGTTCCATCATTTGTAACAACGCTGGTGCATTGTTTGTCCACGCCACAGCAGAAAACACTACCATTGCCGACATACTTCAACCAAAGTATGTTCCTCCCATTGTTTCTTCATTATTCTTACTCAATGATGTGAAAAGCTACGAAGGCACGTCACAACCACTACTTGCAGTACAAGTAACAGAGTTAATCGACGGTATCTTCATTGGTTTTGCAATTAACCATGTGTGTGTAGACGGCAAGTCATTTTGGCATTTCATCAACTCTTGGGCTGAAATCTCACAAGGTTCCAATAAGCCAACAAAAGTACCTTCTTTCGAACGTTGGTTTCCAAACAATGTTAACCTTCCCATACGGTTTCCTTTCACAAAGGATGCACAAAATCAACAATGTGAAGTTTCGTCTAATCGAATCTTCCACTTTACAAAGGAACAAATTCTGCAACTCAAATCAAAAGCTAATACAGAAATTAGTAGTAGTAGTTGTAGTAGTAGTAGTAGTAGTAATAGTAATGATAAAATAATAACGATATCTTCATTGCAAGCGCTTTTAAGTCACCTTTGGCGTTTAATTATAAGTAAACAAAATCTTAAACCAGAAGAAAGGTCTGCATTCGTCTTACCCATAGATTGTAGGACAAGAATGTGTCCAAAGTTAGAAGAGAATTATTTTGGGAGTGGAATCGGAGCAACTGGATATGTAATAATGCAAAAAGGGGAATTAATGGAAAGTGGGATTCGTAAAATTGCTATGGAAATGAACAAGGAGATTTCATCAAAATCTCATCATGAGAAGCTAATGAGTGATATTGAATCTTGGTTGAAAACCCCGTTTATATCGGAGCCAGGTAGATCATCAAGTAATCTTTTGATAGCAAGCAGTTCGCCACGGTTCAACTATTACGGTAATGACTTTGGTTGGGGTAAGCCTATTGCAGTTAGAAATGGGGTTGGACTGCAAAGAAATATTGGTAGAGTTATTGTGAATGGTGGGGCAGAAGAAGGTAGTGTTGATATTGAAGTTTGTCTTCCTTATGATATTTTGGAGGCTATAGAAGACGACCCTATGTCCATGGATTCTAGTTTCATCAACAATGCTATTGAAACACATGCTTGA

Protein sequence

>MS.gene50420.t1

MTEIQLISTSTIQATNHGDGDGNSTHKIVHLSPWDLKFLKLAPTQRGLLFHKPKTNQIDHLKQTLASTLNFFPPLAGRLVITQHDEPSNASCSIICNNAGALFVHATAENTTIADILQPKYVPPIVSSLFLLNDVKSYEGTSQPLLAVQVTELIDGIFIGFAINHVCVDGKSFWHFINSWAEISQGSNKPTKVPSFERWFPNNVNLPIRFPFTKDAQNQQCEVSSNRIFHFTKEQILQLKSKANTEISSSSCSSSSSSNSNDKIITISSLQALLSHLWRLIISKQNLKPEERSAFVLPIDCRTRMCPKLEENYFGSGIGATGYVIMQKGELMESGIRKIAMEMNKEISSKSHHEKLMSDIESWLKTPFISEPGRSSSNLLIASSSPRFNYYGNDFGWGKPIAVRNGVGLQRNIGRVIVNGGAEEGSVDIEVCLPYDILEAIEDDPMSMDSSFINNAIETHA