AlfalfaGEDB | MS.gene46265

M. sativa cultivar XinJiangDaYe / MS.gene46265

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46265.t1	XP_003602051.1	94.2	433	25	0	1	433	1	433	4.80E-245	856.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46265.t1	Q9FH97	40.2	438	237	11	6	434	12	433	1.6e-79	298.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46265.t1	G7J4Y9	94.2	433	25	0	1	433	1	433	3.4e-245	856.7

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene007831	MS.gene46265	PPI
MS.gene008574	MS.gene46265	PPI
MS.gene011796	MS.gene46265	PPI
MS.gene011801	MS.gene46265	PPI
MS.gene011837	MS.gene46265	PPI
MS.gene012960	MS.gene46265	PPI
MS.gene013787	MS.gene46265	PPI
MS.gene021916	MS.gene46265	PPI
MS.gene03202	MS.gene46265	PPI
MS.gene046246	MS.gene46265	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MS.gene46265.t1	MTR_3g088420	94.226	433	25	0	1	433	1	433	857
MS.gene46265.t1	MTR_3g026310	72.098	448	110	3	1	433	1	448	658
MS.gene46265.t1	MTR_4g022800	68.552	442	130	3	1	433	1	442	612
MS.gene46265.t1	MTR_4g022670	67.347	441	136	2	1	433	1	441	609
MS.gene46265.t1	MTR_4g022825	67.273	440	137	1	1	433	1	440	605
MS.gene46265.t1	MTR_4g022720	68.046	435	137	2	1	433	1	435	600
MS.gene46265.t1	MTR_7g026860	66.440	441	137	3	1	433	1	438	585
MS.gene46265.t1	MTR_8g041770	61.486	444	157	6	1	433	1	441	533
MS.gene46265.t1	MTR_0190s0020	61.036	444	160	6	1	433	1	442	530
MS.gene46265.t1	MTR_4g022760	60.091	441	124	3	1	433	1	397	517

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46265.t1	AT5G67160	40.183	438	237	11	6	434	12	433	1.30e-97	299
MS.gene46265.t1	AT5G67150	38.546	454	252	11	1	434	1	447	2.18e-96	296
MS.gene46265.t1	AT3G50300	42.056	428	230	11	1	414	1	424	1.11e-95	295
MS.gene46265.t1	AT3G50280	37.978	445	256	11	1	430	1	440	2.87e-91	283
MS.gene46265.t1	AT3G50270	39.220	436	249	11	12	434	17	449	7.46e-90	280
MS.gene46265.t1	AT5G38130	40.233	430	233	12	1	414	21	442	2.99e-87	273
MS.gene46265.t1	AT5G42830	36.636	434	257	10	15	434	21	450	8.53e-84	264
MS.gene46265.t1	AT5G07860	36.807	451	257	14	2	432	6	448	1.03e-77	249
MS.gene46265.t1	AT5G07870	36.364	462	258	14	2	433	6	461	2.05e-76	246
MS.gene46265.t1	AT5G07850	34.934	458	262	15	2	433	6	453	3.70e-69	226

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Find 91 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCACGGGGAATTGCTAT+TGG	0.430657	3.1:-71272999	None:intergenic
AAACCTGTGGCAATTCGCAG+TGG	0.640198	3.1:+71273052	MS.gene46265:CDS
AAATAATCTTCAGGCAATGG+TGG	0.571644	3.1:-71272776	None:intergenic
AAATGCTGTTGAAGTTTGTA+GGG	0.553867	3.1:+71272802	MS.gene46265:CDS
AAATGGAATAACCTCTCTGA+TGG	0.540722	3.1:-71272572	None:intergenic
AACCTTGATCTAGCTCCTAT+CGG	0.500296	3.1:-71272749	None:intergenic
AACGGATCACAGAGCGCCAT+AGG	0.575889	3.1:-71272685	None:intergenic
AACTCATAACGCTAGAACGT+TGG	0.541457	3.1:+71272462	MS.gene46265:CDS
AATCACTACGAGTCTTGGTT+GGG	0.505413	3.1:+71272926	MS.gene46265:CDS
AATGAAAGCAGGACAGTTGT+TGG	0.413215	3.1:+71272829	MS.gene46265:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATTCAATCCATCAGAG+AGG	+	chr3.1:71272561-71272580	MS.gene46265:CDS	30.0%
AAATGCTGTTGAAGTTTGTA+GGG	+	chr3.1:71272802-71272821	MS.gene46265:CDS	30.0%
ATAGTGAAAGGAAATCGTAT+GGG	-	chr3.1:71272509-71272528	None:intergenic	30.0%
GCATTTCCAAAATAATCTTC+AGG	-	chr3.1:71272788-71272807	None:intergenic	30.0%
TATAGTGAAAGGAAATCGTA+TGG	-	chr3.1:71272510-71272529	None:intergenic	30.0%
TTACCATAAACATCAAACCA+CGG	-	chr3.1:71273016-71273035	None:intergenic	30.0%
AAATAATCTTCAGGCAATGG+TGG	-	chr3.1:71272779-71272798	None:intergenic	35.0%
AAATGGAATAACCTCTCTGA+TGG	-	chr3.1:71272575-71272594	None:intergenic	35.0%
ACTGAAATTGAAAGCCAACA+TGG	+	chr3.1:71272616-71272635	MS.gene46265:CDS	35.0%
CCAAAATAATCTTCAGGCAA+TGG	-	chr3.1:71272782-71272801	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	71271906	71273210	71271906	ID=MS.gene46265
chr3.1	mRNA	71271906	71273210	71271906	ID=MS.gene46265.t1;Parent=MS.gene46265
chr3.1	exon	71271906	71273210	71271906	ID=MS.gene46265.t1.exon1;Parent=MS.gene46265.t1
chr3.1	CDS	71271906	71273210	71271906	ID=cds.MS.gene46265.t1;Parent=MS.gene46265.t1

Gene Sequence

>MS.gene46265

ATGGCTGCAATCCAAGTCCTCTCTACCCATACAATCAAGTCACCAAATTCAATTGATCAAAAAATTGATTTAACTCCATGGGATCTCCGGTACCTACTAATTGCACCCACCAAAAAGGGTCTTCTTTATCACCATCCTCTAGTGCCAAATCAAATTCAACATCTGAAACACTCTCTATCTTCTGCCCTTGCCTTTTTCCCACCACTTGCAGGCCGTCTCGAAATAACAGATCACAAAGACAACACCATTTCATGTTCCGTCACATGCAACAATGCTGGTGCGCTCTTTGTCCATGCCGCAGCGGAGAACAATTGCGTAGGTGACATACTTGGATGCACCTACGTTCCTCCCATCGTCGATTCATTTTTTCCATTAATCGGAGTTCAAAACTACGAAGGCACGTCACAGCCATTGTTAGCTGTGCAAGTGACTGAGTTAGTTGACGGCATCTTCATTGGTTTTACATTTAACCACACGGTTGTTGACGGAGAGTCCACATGGCTTTTCATTAACTCATGGGCTAAAATCTCGCGTGGTTGTTGCAATCAAGTATCCAAACTCATAACGCTAGAACGTTGGTTTCCAAGTGGTATTCAACATCCCATACGATTTCCTTTCACTATAGAGCCACAAAAGAATGACTCTGATGATAACGAAAAATTCAATCCATCAGAGAGGTTATTCCATTTCCCAAAGGAGAAAATCGCTCAACTGAAATTGAAAGCCAACATGGAGATTGGTACCAACAAAATATCATCCCTCCAAGCACTTTTTACACACCTATGGCGCTCTGTGATCCGTTCCAAACAATTTCACCCACACGAAGAAGTTTATTATATGGTCCCGATAGGAGCTAGATCAAGGTTTGTTCCACCATTGCCTGAAGATTATTTTGGAAATGCTGTTGAAGTTTGTAGGGTTACAATGAAAGCAGGACAGTTGTTGGAAGATGGTGGGCTTGGTAAAGGTGCTTGGGCGATACACAAAATGATTTCTTTGCAGTCAAATGAGAAGTTGAAGAATCACTACGAGTCTTGGTTGGGAAATCCAAACATTGTTAGATTTTTTAGTGCGACCAATAATAATACATTAGCCAATAGCAATTCCCCGTGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAACCTGTGGCAATTCGCAGTGGAAATAAAATAAGTGGAATGATTACTGTATTTGCTGGAAAAGAAGAAGGTAGTATGGACTTTCAAGTGTGTCTTCCTCACAAGATTTTGGAGGCAATGGGAAATGACCATGACTTCATGGATGTTGTGTCTATTTAA

Protein sequence

>MS.gene46265.t1

MAAIQVLSTHTIKSPNSIDQKIDLTPWDLRYLLIAPTKKGLLYHHPLVPNQIQHLKHSLSSALAFFPPLAGRLEITDHKDNTISCSVTCNNAGALFVHAAAENNCVGDILGCTYVPPIVDSFFPLIGVQNYEGTSQPLLAVQVTELVDGIFIGFTFNHTVVDGESTWLFINSWAKISRGCCNQVSKLITLERWFPSGIQHPIRFPFTIEPQKNDSDDNEKFNPSERLFHFPKEKIAQLKLKANMEIGTNKISSLQALFTHLWRSVIRSKQFHPHEEVYYMVPIGARSRFVPPLPEDYFGNAVEVCRVTMKAGQLLEDGGLGKGAWAIHKMISLQSNEKLKNHYESWLGNPNIVRFFSATNNNTLANSNSPWFDVYGNDFGWGKPVAIRSGNKISGMITVFAGKEEGSMDFQVCLPHKILEAMGNDHDFMDVVSI