AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56799

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56799.t1 MTR_4g094842 91.727 411 31 2 1 410 39 447 0.0 772
MS.gene56799.t1 MTR_4g094835 61.463 410 140 7 3 407 46 442 2.94e-178 504
MS.gene56799.t1 MTR_8g041770 58.293 410 158 5 1 409 42 439 2.52e-166 474
MS.gene56799.t1 MTR_4g022825 57.869 413 154 7 2 407 37 436 1.24e-164 469
MS.gene56799.t1 MTR_4g094838 59.367 411 137 7 3 407 42 428 4.05e-164 468
MS.gene56799.t1 MTR_4g094832 63.944 355 116 5 58 407 79 426 1.29e-162 464
MS.gene56799.t1 MTR_0190s0020 55.257 409 174 4 1 409 41 440 6.17e-162 462
MS.gene56799.t1 MTR_3g088420 57.561 410 155 8 3 409 38 431 9.01e-160 457
MS.gene56799.t1 MTR_8g079710 57.664 411 146 9 3 402 43 436 2.16e-159 456
MS.gene56799.t1 MTR_4g022720 58.252 412 153 8 2 409 37 433 3.39e-159 456
MS.gene56799.t1 MTR_4g022800 56.938 418 156 8 2 409 37 440 1.16e-158 454
MS.gene56799.t1 MTR_4g022670 56.355 417 159 8 2 409 37 439 2.23e-157 451
MS.gene56799.t1 MTR_7g026860 56.731 416 156 8 2 409 37 436 9.68e-157 449
MS.gene56799.t1 MTR_3g026310 55.107 421 161 8 3 408 38 445 3.54e-155 446
MS.gene56799.t1 MTR_4g094828 54.126 412 166 8 3 403 43 442 9.37e-155 445
MS.gene56799.t1 MTR_4g022290 54.106 414 159 9 3 404 44 438 3.09e-150 433
MS.gene56799.t1 MTR_4g022760 51.442 416 137 7 2 409 37 395 7.22e-134 390
MS.gene56799.t1 MTR_3g101900 42.754 414 215 13 3 407 44 444 2.29e-98 301
MS.gene56799.t1 MTR_6g016815 44.359 390 140 8 19 407 64 377 5.96e-97 295
MS.gene56799.t1 MTR_3g101930 42.381 420 219 11 3 409 44 453 6.47e-97 297
MS.gene56799.t1 MTR_3g101960 41.063 414 223 9 3 406 43 445 2.98e-89 278
MS.gene56799.t1 MTR_3g101970 40.580 414 216 11 2 407 42 433 1.58e-87 273
MS.gene56799.t1 MTR_3g102020 40.144 416 227 10 3 407 43 447 3.32e-86 270
MS.gene56799.t1 MTR_3g102010 38.795 415 233 9 3 407 44 447 9.39e-82 258
MS.gene56799.t1 MTR_3g070170 35.666 443 224 9 3 407 47 466 1.16e-81 258
MS.gene56799.t1 MTR_8g040780 54.419 215 95 2 195 406 13 227 1.33e-78 243
MS.gene56799.t1 MTR_5g081960 33.405 464 228 10 3 407 41 482 6.26e-72 234
MS.gene56799.t1 MTR_4g015180 32.976 467 229 10 3 408 41 484 6.38e-69 226
MS.gene56799.t1 MTR_7g026850 61.039 154 48 3 2 154 20 162 2.91e-58 188
MS.gene56799.t1 MTR_6g045553 32.579 442 244 12 2 407 48 471 4.19e-58 197
MS.gene56799.t1 MTR_8g040760 56.831 183 70 5 2 183 40 214 9.73e-58 188
MS.gene56799.t1 MTR_4g094825 54.335 173 72 3 3 175 106 271 9.22e-55 186
MS.gene56799.t1 MTR_4g094825 58.278 151 51 2 258 407 248 387 1.18e-51 178
MS.gene56799.t1 MTR_7g026770 48.969 194 73 2 116 307 132 301 8.62e-49 168
MS.gene56799.t1 MTR_3g102030 35.294 306 160 8 65 365 48 320 1.02e-46 163
MS.gene56799.t1 MTR_6g034495 61.157 121 44 1 33 153 43 160 3.98e-45 154
MS.gene56799.t1 MTR_6g034485 56.250 128 52 2 161 288 1 124 1.79e-44 150
MS.gene56799.t1 MTR_4g007540 28.058 417 248 17 8 398 34 424 2.94e-37 140
MS.gene56799.t1 MTR_2g105330 28.087 413 252 17 8 398 34 423 2.43e-35 135
MS.gene56799.t1 MTR_2g064495 27.845 413 253 17 8 398 34 423 4.77e-35 134
MS.gene56799.t1 MTR_7g026830 62.222 90 34 0 51 140 8 97 3.03e-33 120
MS.gene56799.t1 MTR_8g096530 30.682 352 205 13 22 360 57 382 4.46e-33 129
MS.gene56799.t1 MTR_8g075610 27.179 390 248 15 24 400 47 413 1.07e-32 127
MS.gene56799.t1 MTR_3g098980 27.926 376 241 11 24 392 66 418 8.13e-31 122
MS.gene56799.t1 MTR_3g026950 27.529 425 239 15 12 396 42 437 3.59e-29 118
MS.gene56799.t1 MTR_4g013050 26.942 412 226 12 15 402 44 404 5.22e-24 103
MS.gene56799.t1 MTR_1g081660 24.672 381 243 13 8 374 57 407 5.94e-24 103
MS.gene56799.t1 MTR_4g127650 26.492 419 252 19 6 392 46 440 6.85e-24 103
MS.gene56799.t1 MTR_4g097090 25.952 420 251 14 7 392 47 440 7.53e-24 103
MS.gene56799.t1 MTR_3g049150 27.295 403 245 17 21 396 52 433 1.03e-23 102
MS.gene56799.t1 MTR_7g027175 25.635 394 260 12 15 398 59 429 2.47e-22 98.6
MS.gene56799.t1 MTR_7g014330 28.395 405 221 18 7 372 53 427 3.69e-22 98.6
MS.gene56799.t1 MTR_1g040195 27.273 352 222 14 64 398 71 405 4.16e-22 98.2
MS.gene56799.t1 MTR_7g084940 28.255 361 218 15 15 360 58 392 2.37e-21 95.9
MS.gene56799.t1 MTR_7g014250 27.764 407 225 18 7 374 53 429 1.46e-20 93.6
MS.gene56799.t1 MTR_2g089765 27.187 320 186 13 112 395 159 467 1.63e-20 93.6
MS.gene56799.t1 MTR_7g026780 44.037 109 36 3 301 409 26 109 1.76e-20 86.7
MS.gene56799.t1 MTR_6g015315 27.653 311 198 11 113 401 140 445 2.24e-20 93.2
MS.gene56799.t1 MTR_1g040215 25.341 367 228 15 24 375 64 399 2.77e-20 92.8
MS.gene56799.t1 MTR_1g040175 24.791 359 240 12 24 375 64 399 5.72e-20 92.0
MS.gene56799.t1 MTR_3g071320 26.384 307 191 11 6 295 42 330 8.97e-20 91.3
MS.gene56799.t1 MTR_7g014230 27.604 384 225 16 24 372 64 429 9.77e-20 91.3
MS.gene56799.t1 MTR_6g015320 26.059 307 201 10 113 398 143 444 1.25e-19 90.9
MS.gene56799.t1 MTR_6g015850 26.710 307 200 8 113 399 147 448 1.95e-19 90.5
MS.gene56799.t1 MTR_6g015815 24.505 404 263 12 21 400 67 452 2.07e-19 90.1
MS.gene56799.t1 MTR_7g085930 23.799 437 263 13 6 398 41 451 2.36e-19 90.1
MS.gene56799.t1 MTR_6g015830 24.020 408 260 11 21 400 67 452 2.70e-19 90.1
MS.gene56799.t1 MTR_7g015860 24.764 424 250 15 24 403 64 462 1.69e-18 87.4
MS.gene56799.t1 MTR_4g055170 27.687 307 188 10 14 308 59 343 2.18e-18 87.0
MS.gene56799.t1 MTR_3g028210 24.069 403 265 14 17 392 60 448 4.40e-18 86.3
MS.gene56799.t1 MTR_3g086020 23.691 401 245 14 6 392 43 396 5.35e-18 85.9
MS.gene56799.t1 MTR_5g076700 22.871 411 277 16 2 392 36 426 1.09e-17 85.1
MS.gene56799.t1 MTR_3g465980 26.263 297 203 10 1 291 43 329 1.32e-17 84.7
MS.gene56799.t1 MTR_7g026800 37.143 140 58 2 189 327 27 137 2.20e-17 80.5
MS.gene56799.t1 MTR_8g012750 26.203 374 226 13 24 360 62 422 2.80e-17 84.0
MS.gene56799.t1 MTR_3g087640 26.150 413 250 16 24 398 67 462 3.01e-17 84.0
MS.gene56799.t1 MTR_7g086000 26.914 405 253 17 24 400 60 449 4.44e-17 83.2
MS.gene56799.t1 MTR_7g114550 23.846 390 259 15 2 374 36 404 7.50e-17 82.4
MS.gene56799.t1 MTR_3g049140 26.957 230 133 7 185 396 18 230 9.50e-17 79.7
MS.gene56799.t1 MTR_5g090830 25.945 397 223 15 7 361 45 412 1.93e-16 81.3
MS.gene56799.t1 MTR_7g085990 23.690 439 265 14 6 400 37 449 2.23e-16 81.3
MS.gene56799.t1 MTR_5g090810 25.266 376 214 14 24 361 66 412 2.74e-16 80.9
MS.gene56799.t1 MTR_7g114470 24.235 425 255 19 2 392 37 428 4.19e-16 80.1
MS.gene56799.t1 MTR_5g084850 23.650 389 242 16 6 377 41 391 4.21e-16 80.1
MS.gene56799.t1 MTR_7g014160 26.764 411 235 19 24 392 69 455 5.02e-16 80.1
MS.gene56799.t1 MTR_7g114520 23.724 392 264 15 9 386 53 423 6.54e-16 79.7
MS.gene56799.t1 MTR_5g026320 23.487 413 254 14 21 406 71 448 7.26e-16 79.7
MS.gene56799.t1 MTR_7g014360 27.372 274 155 11 113 363 90 342 7.49e-16 79.0
MS.gene56799.t1 MTR_1g053315 23.611 360 226 16 24 361 78 410 7.71e-16 79.3
MS.gene56799.t1 MTR_2g089755 24.468 376 232 13 24 363 73 432 8.52e-16 79.3
MS.gene56799.t1 MTR_6g053810 24.823 282 194 8 18 292 65 335 9.08e-16 79.3
MS.gene56799.t1 MTR_8g079970 21.772 395 249 13 6 377 41 398 9.66e-16 79.0
MS.gene56799.t1 MTR_3g465990 26.389 288 189 10 13 291 56 329 1.72e-15 78.6
MS.gene56799.t1 MTR_3g450710 24.235 392 234 15 7 361 46 411 3.55e-15 77.4
MS.gene56799.t1 MTR_7g114560 23.529 391 259 16 2 374 36 404 5.02e-15 77.0
MS.gene56799.t1 MTR_7g084910 26.633 398 231 18 15 393 59 414 6.17e-15 76.6
MS.gene56799.t1 MTR_2g089670 27.322 366 219 16 24 360 82 429 6.92e-15 76.6
MS.gene56799.t1 MTR_5g084870 21.374 393 247 13 6 377 41 392 6.95e-15 76.6
MS.gene56799.t1 MTR_7g056087 22.760 413 274 15 7 398 41 429 1.97e-14 75.1
MS.gene56799.t1 MTR_2g089650 24.586 423 260 19 3 388 43 443 2.52e-14 74.7
MS.gene56799.t1 MTR_5g090880 23.969 388 239 15 8 361 49 414 4.80e-14 73.9
MS.gene56799.t1 MTR_8g079950 23.250 400 251 14 6 384 41 405 6.22e-14 73.6
MS.gene56799.t1 MTR_3g465930 24.643 280 193 6 19 291 64 332 2.54e-13 71.6
MS.gene56799.t1 MTR_1g040315 24.916 297 178 12 2 279 35 305 1.52e-12 69.3
MS.gene56799.t1 MTR_7g014620 24.155 414 230 13 24 398 54 422 2.39e-12 68.6
MS.gene56799.t1 MTR_7g026880 51.923 52 23 1 358 409 2 51 3.15e-12 61.6
MS.gene56799.t1 MTR_1g094790 24.700 417 258 16 16 407 52 437 6.19e-12 67.4
MS.gene56799.t1 MTR_3g465910 25.623 281 187 9 19 291 63 329 1.46e-11 66.6
MS.gene56799.t1 MTR_2g015430 23.162 272 196 8 104 369 128 392 1.87e-11 65.9
MS.gene56799.t1 MTR_7g026870 53.704 54 25 0 267 320 98 151 1.92e-11 62.4
MS.gene56799.t1 MTR_7g014200 22.565 421 255 19 21 398 66 458 2.03e-11 65.9
MS.gene56799.t1 MTR_2g007880 23.716 409 253 17 24 408 74 447 7.95e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56799.t1 AT5G67150 42.754 414 214 13 3 407 43 442 1.16e-101 309
MS.gene56799.t1 AT3G50300 42.040 402 211 11 3 396 41 428 6.45e-91 281
MS.gene56799.t1 AT3G50270 39.806 412 231 10 3 407 43 444 7.14e-91 281
MS.gene56799.t1 AT3G50280 39.320 412 230 9 3 407 41 439 1.39e-90 281
MS.gene56799.t1 AT5G67160 39.609 409 218 11 3 406 43 427 4.57e-85 266
MS.gene56799.t1 AT5G38130 40.000 400 214 12 3 393 61 443 7.83e-85 266
MS.gene56799.t1 AT5G42830 38.129 417 235 10 3 409 44 447 3.94e-83 261
MS.gene56799.t1 AT5G07850 38.756 418 234 9 3 409 45 451 4.13e-83 262
MS.gene56799.t1 AT5G07860 38.313 415 236 9 3 409 45 447 4.14e-82 259
MS.gene56799.t1 AT5G07870 37.529 429 232 12 3 409 45 459 2.08e-79 253
MS.gene56799.t1 AT5G01210 35.586 444 230 7 3 408 41 466 3.96e-79 252
MS.gene56799.t1 AT2G39980 34.066 455 231 8 3 408 43 477 2.39e-78 250
MS.gene56799.t1 AT3G50280 38.339 313 184 4 100 407 1 309 9.50e-69 220
MS.gene56799.t1 AT5G23940 32.783 424 254 10 2 407 38 448 1.47e-65 217
MS.gene56799.t1 AT3G50290 42.593 270 143 7 97 358 4 269 1.19e-57 190
MS.gene56799.t1 AT5G48930 28.358 402 254 14 8 392 34 418 8.31e-37 139
MS.gene56799.t1 AT3G48720 25.564 399 272 9 7 399 44 423 3.10e-28 115
MS.gene56799.t1 AT2G19070 25.653 421 270 13 6 400 40 443 2.03e-26 110
MS.gene56799.t1 AT5G41040 27.027 407 248 16 21 409 66 441 3.18e-25 107
MS.gene56799.t1 AT5G41040 27.027 407 248 16 21 409 82 457 4.06e-25 107
MS.gene56799.t1 AT5G57840 27.187 423 243 17 1 392 34 422 3.20e-24 104
MS.gene56799.t1 AT3G29590 25.000 400 249 15 30 402 67 442 1.81e-23 102
MS.gene56799.t1 AT5G63560 24.937 397 259 11 7 392 44 412 6.09e-23 100
MS.gene56799.t1 AT2G40230 26.286 350 235 9 23 365 58 391 7.10e-23 100
MS.gene56799.t1 AT5G17540 27.110 391 239 17 2 372 35 399 2.14e-22 99.4
MS.gene56799.t1 AT1G03940 25.243 412 246 15 21 395 63 449 3.56e-22 98.6
MS.gene56799.t1 AT3G29635 25.765 392 248 14 24 392 67 438 5.52e-22 97.8
MS.gene56799.t1 AT3G03480 26.617 402 237 16 2 380 45 411 1.10e-21 97.1
MS.gene56799.t1 AT5G61160 25.949 316 191 12 110 398 139 438 1.15e-20 94.0
MS.gene56799.t1 AT5G07080 27.063 303 186 12 4 291 49 331 5.53e-20 92.0
MS.gene56799.t1 AT1G65450 26.688 311 201 13 64 360 103 400 4.50e-19 89.4
MS.gene56799.t1 AT1G24430 24.615 390 246 16 6 384 45 397 1.97e-18 87.4
MS.gene56799.t1 AT3G47170 27.402 281 177 10 28 291 74 344 4.74e-18 86.3
MS.gene56799.t1 AT1G24420 25.389 386 243 16 34 408 75 426 4.93e-18 85.9
MS.gene56799.t1 AT1G24420 25.389 386 243 16 34 408 75 426 4.93e-18 85.9
MS.gene56799.t1 AT5G39050 24.252 301 187 11 124 393 160 450 1.13e-17 85.1
MS.gene56799.t1 AT5G16410 26.121 379 237 12 24 384 91 444 1.54e-17 84.7
MS.gene56799.t1 AT3G30280 23.913 414 233 16 5 384 43 408 2.74e-17 84.0
MS.gene56799.t1 AT5G23970 26.238 404 226 19 16 392 44 402 4.72e-17 83.2
MS.gene56799.t1 AT3G26040 24.228 421 266 14 6 408 41 426 6.88e-17 82.8
MS.gene56799.t1 AT5G39080 24.570 407 249 17 21 392 62 445 2.15e-16 81.3
MS.gene56799.t1 AT1G32910 25.668 374 227 16 12 361 53 399 2.66e-16 80.9
MS.gene56799.t1 AT3G29680 25.681 257 164 9 124 361 152 400 1.64e-15 78.6
MS.gene56799.t1 AT4G15390 22.430 428 270 15 5 407 45 435 3.70e-15 77.4
MS.gene56799.t1 AT4G15390 22.430 428 270 15 5 407 45 435 3.70e-15 77.4
MS.gene56799.t1 AT1G03390 24.259 371 243 14 7 361 59 407 6.17e-15 76.6
MS.gene56799.t1 AT1G65450 26.939 245 159 9 127 360 1 236 4.18e-14 72.8
MS.gene56799.t1 AT1G28680 24.069 349 229 15 27 363 65 389 1.51e-13 72.4
MS.gene56799.t1 AT2G23510 23.698 384 268 16 4 378 48 415 4.47e-13 70.9
MS.gene56799.t1 AT1G28680 24.671 304 198 13 27 320 65 347 1.54e-12 68.9

Find 71 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCAAGGTTTGATGTTTA+TGG 0.150931 8.2:+25456326 MS.gene56799:CDS
TTGTCTTTCTTATGATATTT+TGG 0.223517 8.2:+25456463 MS.gene56799:CDS
ATTTGGTTTGAGGTGTCTTT+TGG 0.287416 8.2:-25455333 None:intergenic
GATGTTTATGGTAATGATTT+TGG 0.307637 8.2:+25456338 MS.gene56799:CDS
TTACTTCGGAAATGCCTTGC+AGG 0.351265 8.2:+25456112 MS.gene56799:CDS
CAAGTTACTGAGCTTGTTGA+TGG 0.358436 8.2:+25455642 MS.gene56799:CDS
TTGTCGATGGTAAGTCGTTT+TGG 0.363142 8.2:+25455697 MS.gene56799:CDS
TTACTTGCTGAAGGAGCGTT+TGG 0.366525 8.2:+25456164 MS.gene56799:CDS
ACAATGGAAGGAACATAGTT+TGG 0.370467 8.2:-25455552 None:intergenic
CAATACATGATTTCTTCTTC+CGG 0.374442 8.2:-25456041 None:intergenic
TTTGGCATTTCATCGATTCA+TGG 0.396555 8.2:+25455715 MS.gene56799:CDS
GCTTAGTAGTGTTAAGGCTT+TGG 0.398296 8.2:+25456292 MS.gene56799:CDS
TGTTTAGTCATTGATGTTAG+AGG 0.399942 8.2:+25456062 MS.gene56799:CDS
CAAGTTGAAATGAACCAAAA+AGG 0.407254 8.2:+25455285 None:intergenic
TGCATCCTTTGTAACAATCT+AGG 0.410406 8.2:+25455474 MS.gene56799:CDS
AGGTAGGCTTAGTAGTGTTA+AGG 0.422729 8.2:+25456286 MS.gene56799:CDS
TAAAAGATATGTATAAATTG+AGG 0.446283 8.2:-25456503 None:intergenic
GGCATTTCCGAAGTAATTCT+CGG 0.456049 8.2:-25456105 None:intergenic
AGCCTGTTGCTGTTAGAAGC+GGG 0.459507 8.2:+25456369 MS.gene56799:CDS
GAGGTGTTGGATTTGGTTTG+AGG 0.469311 8.2:-25455343 None:intergenic
AAAAGATATGTATAAATTGA+GGG 0.476845 8.2:-25456502 None:intergenic
ATCAATCATGTTGTTGTCGA+TGG 0.491424 8.2:+25455684 MS.gene56799:CDS
CTTTGGCAAGCTGTGATTCG+TGG 0.491749 8.2:+25456005 MS.gene56799:CDS
TTAAGTGTTGGAAGCTTCGA+TGG 0.495846 8.2:-25455765 None:intergenic
AGCTTCCAACACTTAATCGT+TGG 0.496175 8.2:+25455772 MS.gene56799:CDS
GTGAAATGGAAAATCCTCTC+AGG 0.496490 8.2:-25455894 None:intergenic
GGAACGATAACTATGTTTAG+TGG 0.508616 8.2:+25456410 MS.gene56799:CDS
GAACGATAACTATGTTTAGT+GGG 0.510514 8.2:+25456411 MS.gene56799:CDS
AGCCTACCTTGAAACAACCT+TGG 0.510659 8.2:-25456272 None:intergenic
CCGAAGTAATTCTCGGGCAA+AGG 0.515823 8.2:-25456098 None:intergenic
ATGTTTAGTGGGGCAGAAGA+AGG 0.527980 8.2:+25456422 MS.gene56799:CDS
AGAAAACAAGAGTTGATGAA+AGG 0.528232 8.2:-25455374 None:intergenic
GAAAACAAGAGTTGATGAAA+GGG 0.528741 8.2:-25455373 None:intergenic
GGAAATGCCTTGCAGGTTGG+TGG 0.536401 8.2:+25456119 MS.gene56799:CDS
ATGAGACGACCGGTGAGGGG+TGG 0.536608 8.2:-25455399 None:intergenic
TGAGGAGAAACTATGAAAGT+TGG 0.536823 8.2:+25456240 MS.gene56799:CDS
GCATTTCCGAAGTAATTCTC+GGG 0.542365 8.2:-25456104 None:intergenic
AAGTAATTCTCGGGCAAAGG+TGG 0.543022 8.2:-25456095 None:intergenic
GAGGAGAAACTATGAAAGTT+GGG 0.544119 8.2:+25456241 MS.gene56799:CDS
AATGATGAGACGACCGGTGA+GGG 0.544298 8.2:-25455403 None:intergenic
CCTTTGCCCGAGAATTACTT+CGG 0.547729 8.2:+25456098 MS.gene56799:CDS
ATCCAAGGTTGTTTCAAGGT+AGG 0.548103 8.2:+25456270 MS.gene56799:CDS
TTCGGAAATGCCTTGCAGGT+TGG 0.554030 8.2:+25456116 MS.gene56799:CDS
TGAGACGACCGGTGAGGGGT+GGG 0.555527 8.2:-25455398 None:intergenic
GCCTGTTGCTGTTAGAAGCG+GGG 0.558922 8.2:+25456370 MS.gene56799:CDS
AAAATTTCCATTCACAAAGG+AGG 0.568603 8.2:+25455824 MS.gene56799:CDS
GTAACTTGAACTGCAAGCAA+TGG 0.569233 8.2:-25455627 None:intergenic
AAGCCTGTTGCTGTTAGAAG+CGG 0.572484 8.2:+25456368 MS.gene56799:CDS
TATAAAATTTCCATTCACAA+AGG 0.573451 8.2:+25455821 MS.gene56799:CDS
AGAAACCAACGATTAAGTGT+TGG 0.580659 8.2:-25455777 None:intergenic
CCTGTTGCTGTTAGAAGCGG+GGG 0.582350 8.2:+25456371 MS.gene56799:CDS
TTACCATAAACATCAAACCT+TGG 0.582755 8.2:-25456329 None:intergenic
GCAAGGGAATTACTTGCTGA+AGG 0.591175 8.2:+25456155 MS.gene56799:CDS
AAATGGAAAATCCTCTCAGG+TGG 0.591822 8.2:-25455891 None:intergenic
TCGTGGTCAGAATATCGATC+CGG 0.596096 8.2:+25456022 MS.gene56799:CDS
TGGCAACTAGTAGTTCTCCA+AGG 0.596495 8.2:+25456312 MS.gene56799:CDS
GTGGTGTGAAAGTGAAAGCA+AGG 0.599974 8.2:+25456138 MS.gene56799:CDS
CACGAATCACAGCTTGCCAA+AGG 0.614383 8.2:-25456004 None:intergenic
ACGCACCTAGATTGTTACAA+AGG 0.626814 8.2:-25455479 None:intergenic
ATGTTCAATGATGAGACGAC+CGG 0.630993 8.2:-25455409 None:intergenic
GTTACAAAGGATGCAACATG+AGG 0.639280 8.2:-25455466 None:intergenic
GAAATCAAAAGCTAACGAAG+AGG 0.643741 8.2:+25455938 MS.gene56799:CDS
TTTCACACCACCAACCTGCA+AGG 0.645939 8.2:-25456126 None:intergenic
GAACATCAAGAAGAAGAAGA+CGG 0.649390 8.2:+25455426 MS.gene56799:CDS
ATGATGAGACGACCGGTGAG+GGG 0.660103 8.2:-25455402 None:intergenic
CAATGATGAGACGACCGGTG+AGG 0.661803 8.2:-25455404 None:intergenic
AACGATAACTATGTTTAGTG+GGG 0.682828 8.2:+25456412 MS.gene56799:CDS
AAAACTCTATTCCACCTGAG+AGG 0.686471 8.2:+25455880 MS.gene56799:CDS
TCATGGTCTGAAATCTCACG+TGG 0.688642 8.2:+25455732 MS.gene56799:CDS
TGGTGTGAAAGTGAAAGCAA+GGG 0.721745 8.2:+25456139 MS.gene56799:CDS
CACATTGTGATGATACTGTG+AGG 0.773763 8.2:+25456222 MS.gene56799:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTTTTTTACTTTATTAAA+CGG - chr8.2:25455591-25455610 None:intergenic 10.0%
!! AAAAGATATGTATAAATTGA+GGG - chr8.2:25456505-25456524 None:intergenic 15.0%
!! TAAAAGATATGTATAAATTG+AGG - chr8.2:25456506-25456525 None:intergenic 15.0%
!! AATAAAGTAAAAAACTACGA+AGG + chr8.2:25455594-25455613 MS.gene56799:CDS 20.0%
!! TATAAAATTTCCATTCACAA+AGG + chr8.2:25455821-25455840 MS.gene56799:CDS 20.0%
!!! AGAAGATATTTTGTTTGTAT+TGG - chr8.2:25455964-25455983 None:intergenic 20.0%
!!! TTGTCTTTCTTATGATATTT+TGG + chr8.2:25456463-25456482 MS.gene56799:CDS 20.0%
!!! TTTGTGAATGGAAATTTTAT+AGG - chr8.2:25455822-25455841 None:intergenic 20.0%
!! TAATTTTCTCCTTTGTGAAA+TGG - chr8.2:25455911-25455930 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr8.2:25456338-25456357 MS.gene56799:CDS 25.0%
!!! TCTTTCTTATGATATTTTGG+AGG + chr8.2:25456466-25456485 MS.gene56799:CDS 25.0%
!!! TTATGGTAATGATTTTGGTT+GGG + chr8.2:25456343-25456362 MS.gene56799:CDS 25.0%
!!! TTTATGGTAATGATTTTGGT+TGG + chr8.2:25456342-25456361 MS.gene56799:CDS 25.0%
AAAATTTCCATTCACAAAGG+AGG + chr8.2:25455824-25455843 MS.gene56799:CDS 30.0%
AACGATAACTATGTTTAGTG+GGG + chr8.2:25456412-25456431 MS.gene56799:CDS 30.0%
CAATACATGATTTCTTCTTC+CGG - chr8.2:25456044-25456063 None:intergenic 30.0%
GAACGATAACTATGTTTAGT+GGG + chr8.2:25456411-25456430 MS.gene56799:CDS 30.0%
TGTTTAGTCATTGATGTTAG+AGG + chr8.2:25456062-25456081 MS.gene56799:CDS 30.0%
TTACCATAAACATCAAACCT+TGG - chr8.2:25456332-25456351 None:intergenic 30.0%
! AAAAATCCAAGGTTGTTTCA+AGG + chr8.2:25456266-25456285 MS.gene56799:CDS 30.0%
! TATGAAAGAGAAGTCCTTTT+TGG - chr8.2:25455302-25455321 None:intergenic 30.0%
! TTATTTTCCTCCTTTGTGAA+TGG - chr8.2:25455834-25455853 None:intergenic 30.0%
!! AGAAAACAAGAGTTGATGAA+AGG - chr8.2:25455377-25455396 None:intergenic 30.0%
!! GAAAACAAGAGTTGATGAAA+GGG - chr8.2:25455376-25455395 None:intergenic 30.0%
!! TATGATATTTTGGAGGCTAT+GGG + chr8.2:25456473-25456492 MS.gene56799:CDS 30.0%
!! TTATGATATTTTGGAGGCTA+TGG + chr8.2:25456472-25456491 MS.gene56799:CDS 30.0%
!!! TATGGTAATGATTTTGGTTG+GGG + chr8.2:25456344-25456363 MS.gene56799:CDS 30.0%
AAAAAAGAGCGAACAATGGA+AGG - chr8.2:25455567-25455586 None:intergenic 35.0%
AAAGTTGGGTGAAAAATCCA+AGG + chr8.2:25456255-25456274 MS.gene56799:CDS 35.0%
ACAATGGAAGGAACATAGTT+TGG - chr8.2:25455555-25455574 None:intergenic 35.0%
AGAAACCAACGATTAAGTGT+TGG - chr8.2:25455780-25455799 None:intergenic 35.0%
ATCAATCATGTTGTTGTCGA+TGG + chr8.2:25455684-25455703 MS.gene56799:CDS 35.0%
GAAATCAAAAGCTAACGAAG+AGG + chr8.2:25455938-25455957 MS.gene56799:CDS 35.0%
GAACATCAAGAAGAAGAAGA+CGG + chr8.2:25455426-25455445 MS.gene56799:CDS 35.0%
GAGGAGAAACTATGAAAGTT+GGG + chr8.2:25456241-25456260 MS.gene56799:CDS 35.0%
GGAACGATAACTATGTTTAG+TGG + chr8.2:25456410-25456429 MS.gene56799:CDS 35.0%
TCTCCAAGGTTTGATGTTTA+TGG + chr8.2:25456326-25456345 MS.gene56799:CDS 35.0%
TGAGGAGAAACTATGAAAGT+TGG + chr8.2:25456240-25456259 MS.gene56799:CDS 35.0%
TGCATCCTTTGTAACAATCT+AGG + chr8.2:25455474-25455493 MS.gene56799:CDS 35.0%
! GAGGATTTTCCATTTCACAA+AGG + chr8.2:25455899-25455918 MS.gene56799:CDS 35.0%
! TTGTGTTTTCAGCTATTGCA+TGG - chr8.2:25455512-25455531 None:intergenic 35.0%
!! AAGCACTTTTAACTCACCTT+TGG + chr8.2:25455988-25456007 MS.gene56799:CDS 35.0%
!! TTTGGCATTTCATCGATTCA+TGG + chr8.2:25455715-25455734 MS.gene56799:CDS 35.0%
!!! ATTTGGTTTGAGGTGTCTTT+TGG - chr8.2:25455336-25455355 None:intergenic 35.0%
!!! CTTGTTGATGGCATTTTCAT+TGG + chr8.2:25455654-25455673 MS.gene56799:CDS 35.0%
!!! TGTTTTTGAGGTGTTGGATT+TGG - chr8.2:25455353-25455372 None:intergenic 35.0%
!!! TTGATGAAAGGGTGTTTTTG+AGG - chr8.2:25455365-25455384 None:intergenic 35.0%
!!! TTTGGTTTGAGGTGTCTTTT+GGG - chr8.2:25455335-25455354 None:intergenic 35.0%
AAAACTCTATTCCACCTGAG+AGG + chr8.2:25455880-25455899 MS.gene56799:CDS 40.0%
AAATGGAAAATCCTCTCAGG+TGG - chr8.2:25455894-25455913 None:intergenic 40.0%
ACGCACCTAGATTGTTACAA+AGG - chr8.2:25455482-25455501 None:intergenic 40.0%
AGCTTCCAACACTTAATCGT+TGG + chr8.2:25455772-25455791 MS.gene56799:CDS 40.0%
AGGTAGGCTTAGTAGTGTTA+AGG + chr8.2:25456286-25456305 MS.gene56799:CDS 40.0%
ATGTTCAATGATGAGACGAC+CGG - chr8.2:25455412-25455431 None:intergenic 40.0%
CACATTGTGATGATACTGTG+AGG + chr8.2:25456222-25456241 MS.gene56799:CDS 40.0%
CGGAAAAAAAGAGCGAACAA+TGG - chr8.2:25455571-25455590 None:intergenic 40.0%
GCATTTCCGAAGTAATTCTC+GGG - chr8.2:25456107-25456126 None:intergenic 40.0%
GCTTAGTAGTGTTAAGGCTT+TGG + chr8.2:25456292-25456311 MS.gene56799:CDS 40.0%
GGCATTTCCGAAGTAATTCT+CGG - chr8.2:25456108-25456127 None:intergenic 40.0%
GTAACTTGAACTGCAAGCAA+TGG - chr8.2:25455630-25455649 None:intergenic 40.0%
GTGAAATGGAAAATCCTCTC+AGG - chr8.2:25455897-25455916 None:intergenic 40.0%
GTTACAAAGGATGCAACATG+AGG - chr8.2:25455469-25455488 None:intergenic 40.0%
TGGTGTGAAAGTGAAAGCAA+GGG + chr8.2:25456139-25456158 MS.gene56799:CDS 40.0%
! ATCCAAGGTTGTTTCAAGGT+AGG + chr8.2:25456270-25456289 MS.gene56799:CDS 40.0%
! TTGTCGATGGTAAGTCGTTT+TGG + chr8.2:25455697-25455716 MS.gene56799:CDS 40.0%
!! CAAGTTACTGAGCTTGTTGA+TGG + chr8.2:25455642-25455661 MS.gene56799:CDS 40.0%
!! TTAAGTGTTGGAAGCTTCGA+TGG - chr8.2:25455768-25455787 None:intergenic 40.0%
!!! AAAGGGTGTTTTTGAGGTGT+TGG - chr8.2:25455359-25455378 None:intergenic 40.0%
!!! GAGGTGTCTTTTGGGTTTTT+GGG - chr8.2:25455327-25455346 None:intergenic 40.0%
!!! TGAGGTGTCTTTTGGGTTTT+TGG - chr8.2:25455328-25455347 None:intergenic 40.0%
AAGTAATTCTCGGGCAAAGG+TGG - chr8.2:25456098-25456117 None:intergenic 45.0%
AGCCTACCTTGAAACAACCT+TGG - chr8.2:25456275-25456294 None:intergenic 45.0%
ATGTTTAGTGGGGCAGAAGA+AGG + chr8.2:25456422-25456441 MS.gene56799:CDS 45.0%
CCTTTGCCCGAGAATTACTT+CGG + chr8.2:25456098-25456117 MS.gene56799:CDS 45.0%
GTGGTGTGAAAGTGAAAGCA+AGG + chr8.2:25456138-25456157 MS.gene56799:CDS 45.0%
TCGTGGTCAGAATATCGATC+CGG + chr8.2:25456022-25456041 MS.gene56799:CDS 45.0%
TGGCAACTAGTAGTTCTCCA+AGG + chr8.2:25456312-25456331 MS.gene56799:CDS 45.0%
TTACTTCGGAAATGCCTTGC+AGG + chr8.2:25456112-25456131 MS.gene56799:CDS 45.0%
! AAGCCTGTTGCTGTTAGAAG+CGG + chr8.2:25456368-25456387 MS.gene56799:CDS 45.0%
! GCAAGGGAATTACTTGCTGA+AGG + chr8.2:25456155-25456174 MS.gene56799:CDS 45.0%
! GGGGGTGCAAATAAAAGTGA+TGG + chr8.2:25456389-25456408 MS.gene56799:CDS 45.0%
! TCATGGTCTGAAATCTCACG+TGG + chr8.2:25455732-25455751 MS.gene56799:CDS 45.0%
!! GAGGTGTTGGATTTGGTTTG+AGG - chr8.2:25455346-25455365 None:intergenic 45.0%
!! TTACTTGCTGAAGGAGCGTT+TGG + chr8.2:25456164-25456183 MS.gene56799:CDS 45.0%
AATGATGAGACGACCGGTGA+GGG - chr8.2:25455406-25455425 None:intergenic 50.0%
CACGAATCACAGCTTGCCAA+AGG - chr8.2:25456007-25456026 None:intergenic 50.0%
CCGAAGTAATTCTCGGGCAA+AGG - chr8.2:25456101-25456120 None:intergenic 50.0%
CTTTGGCAAGCTGTGATTCG+TGG + chr8.2:25456005-25456024 MS.gene56799:CDS 50.0%
TTCGGAAATGCCTTGCAGGT+TGG + chr8.2:25456116-25456135 MS.gene56799:CDS 50.0%
TTTCACACCACCAACCTGCA+AGG - chr8.2:25456129-25456148 None:intergenic 50.0%
! AGCCTGTTGCTGTTAGAAGC+GGG + chr8.2:25456369-25456388 MS.gene56799:CDS 50.0%
ATGATGAGACGACCGGTGAG+GGG - chr8.2:25455405-25455424 None:intergenic 55.0%
CAATGATGAGACGACCGGTG+AGG - chr8.2:25455407-25455426 None:intergenic 55.0%
! CCTGTTGCTGTTAGAAGCGG+GGG + chr8.2:25456371-25456390 MS.gene56799:CDS 55.0%
! GCCTGTTGCTGTTAGAAGCG+GGG + chr8.2:25456370-25456389 MS.gene56799:CDS 55.0%
! GGAAATGCCTTGCAGGTTGG+TGG + chr8.2:25456119-25456138 MS.gene56799:CDS 55.0%
! GTTTTCTTCCCACCCCTCAC+CGG + chr8.2:25455390-25455409 MS.gene56799:CDS 55.0%
CCCCCGCTTCTAACAGCAAC+AGG - chr8.2:25456374-25456393 None:intergenic 60.0%
ATGAGACGACCGGTGAGGGG+TGG - chr8.2:25455402-25455421 None:intergenic 65.0%
TGAGACGACCGGTGAGGGGT+GGG - chr8.2:25455401-25455420 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 25455294 25456526 25455294 ID=MS.gene56799
chr8.2 mRNA 25455294 25456526 25455294 ID=MS.gene56799.t1;Parent=MS.gene56799
chr8.2 exon 25455294 25456526 25455294 ID=MS.gene56799.t1.exon1;Parent=MS.gene56799.t1
chr8.2 CDS 25455294 25456526 25455294 ID=cds.MS.gene56799.t1;Parent=MS.gene56799.t1
Gene Sequence

>MS.gene56799

ATGAACCAAAAAGGACTTCTCTTTCATAATCCCAAAAACCCAAAAGACACCTCAAACCAAATCCAACACCTCAAAAACACCCTTTCATCAACTCTTGTTTTCTTCCCACCCCTCACCGGTCGTCTCATCATTGAACATCAAGAAGAAGAAGACGGCGACAACAACAACACAACCTCATGTTGCATCCTTTGTAACAATCTAGGTGCGTTATTTATCCATGCAATAGCTGAAAACACAAGTGTCTCTGATATTCTTAAACCAAACTATGTTCCTTCCATTGTTCGCTCTTTTTTTCCGTTTAATAAAGTAAAAAACTACGAAGGCACATCAAAACCATTGCTTGCAGTTCAAGTTACTGAGCTTGTTGATGGCATTTTCATTGGCTTTTCAATCAATCATGTTGTTGTCGATGGTAAGTCGTTTTGGCATTTCATCGATTCATGGTCTGAAATCTCACGTGGTTTTGATCGACCATCGAAGCTTCCAACACTTAATCGTTGGTTTCTCGATGACTCAATTGAAATTCCTATAAAATTTCCATTCACAAAGGAGGAAAATAAAGAAAGTACTGAAGCTGAAACTGATGAAAACTCTATTCCACCTGAGAGGATTTTCCATTTCACAAAGGAGAAAATTACGAAACTGAAATCAAAAGCTAACGAAGAGGCCAATACAAACAAAATATCTTCTTTACAAGCACTTTTAACTCACCTTTGGCAAGCTGTGATTCGTGGTCAGAATATCGATCCGGAAGAAGAAATCATGTATTGTTTAGTCATTGATGTTAGAGGCAGAATTATTCCACCTTTGCCCGAGAATTACTTCGGAAATGCCTTGCAGGTTGGTGGTGTGAAAGTGAAAGCAAGGGAATTACTTGCTGAAGGAGCGTTTGGAAAAGTTGCTTTTGAGATGAATAAGATGATAGCTTCACATTGTGATGATACTGTGAGGAGAAACTATGAAAGTTGGGTGAAAAATCCAAGGTTGTTTCAAGGTAGGCTTAGTAGTGTTAAGGCTTTGGCAACTAGTAGTTCTCCAAGGTTTGATGTTTATGGTAATGATTTTGGTTGGGGTAAGCCTGTTGCTGTTAGAAGCGGGGGTGCAAATAAAAGTGATGGAACGATAACTATGTTTAGTGGGGCAGAAGAAGGTAGTATTGATGTTGAAGTTTGTCTTTCTTATGATATTTTGGAGGCTATGGGAAATGACCCTCAATTTATACATATCTTTTAA
Protein sequence

>MS.gene56799.t1

MNQKGLLFHNPKNPKDTSNQIQHLKNTLSSTLVFFPPLTGRLIIEHQEEEDGDNNNTTSCCILCNNLGALFIHAIAENTSVSDILKPNYVPSIVRSFFPFNKVKNYEGTSKPLLAVQVTELVDGIFIGFSINHVVVDGKSFWHFIDSWSEISRGFDRPSKLPTLNRWFLDDSIEIPIKFPFTKEENKESTEAETDENSIPPERIFHFTKEKITKLKSKANEEANTNKISSLQALLTHLWQAVIRGQNIDPEEEIMYCLVIDVRGRIIPPLPENYFGNALQVGGVKVKARELLAEGAFGKVAFEMNKMIASHCDDTVRRNYESWVKNPRLFQGRLSSVKALATSSSPRFDVYGNDFGWGKPVAVRSGGANKSDGTITMFSGAEEGSIDVEVCLSYDILEAMGNDPQFIHIF