AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016532

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016532.t1 MTR_8g040780 89.960 249 23 1 142 388 1 249 8.22e-165 461
MS.gene016532.t1 MTR_4g022825 62.634 372 128 4 7 370 72 440 2.34e-163 465
MS.gene016532.t1 MTR_3g088420 63.333 360 129 2 7 366 72 428 7.37e-163 464
MS.gene016532.t1 MTR_4g022720 60.331 363 141 2 5 366 70 430 8.44e-152 436
MS.gene016532.t1 MTR_8g041770 60.221 362 140 4 5 366 79 436 2.10e-151 435
MS.gene016532.t1 MTR_0190s0020 59.504 363 143 4 5 366 78 437 7.12e-150 431
MS.gene016532.t1 MTR_3g026310 59.140 372 136 5 7 364 72 441 5.98e-148 426
MS.gene016532.t1 MTR_4g022800 59.249 373 140 6 7 371 72 440 6.21e-147 424
MS.gene016532.t1 MTR_4g022670 58.583 367 143 3 7 366 72 436 1.37e-145 420
MS.gene016532.t1 MTR_7g026860 58.038 367 142 4 7 366 72 433 3.71e-143 414
MS.gene016532.t1 MTR_4g094832 55.764 373 147 6 6 366 59 425 1.06e-136 397
MS.gene016532.t1 MTR_4g094835 55.053 376 155 7 5 373 79 447 2.45e-135 394
MS.gene016532.t1 MTR_4g094842 54.054 370 159 5 5 366 77 443 2.09e-132 387
MS.gene016532.t1 MTR_4g022760 52.044 367 123 3 7 366 72 392 1.86e-123 362
MS.gene016532.t1 MTR_4g094838 50.404 371 153 5 5 366 79 427 1.60e-119 353
MS.gene016532.t1 MTR_8g079710 50.954 367 163 9 7 362 76 436 3.40e-112 335
MS.gene016532.t1 MTR_4g094828 48.378 370 175 9 7 363 76 442 1.04e-106 322
MS.gene016532.t1 MTR_4g022290 48.787 371 168 9 7 364 77 438 1.53e-104 315
MS.gene016532.t1 MTR_6g016815 46.027 365 126 7 7 371 86 379 2.03e-94 288
MS.gene016532.t1 MTR_8g040760 92.029 138 11 0 5 142 76 213 7.29e-90 270
MS.gene016532.t1 MTR_3g101970 36.585 369 214 8 5 369 84 436 4.68e-70 227
MS.gene016532.t1 MTR_3g101960 35.849 371 225 9 5 368 83 447 4.40e-69 224
MS.gene016532.t1 MTR_3g102020 36.856 369 220 8 5 366 84 446 6.76e-69 224
MS.gene016532.t1 MTR_3g101900 35.616 365 224 8 5 363 81 440 2.38e-68 223
MS.gene016532.t1 MTR_3g102010 35.171 381 231 9 5 375 80 454 2.49e-65 215
MS.gene016532.t1 MTR_3g101930 35.674 356 218 6 17 366 100 450 6.77e-61 203
MS.gene016532.t1 MTR_5g081960 29.064 406 214 9 22 366 89 481 1.68e-49 174
MS.gene016532.t1 MTR_4g015180 32.020 406 204 11 22 366 88 482 8.98e-49 172
MS.gene016532.t1 MTR_3g070170 29.396 381 224 8 22 366 94 465 2.65e-47 168
MS.gene016532.t1 MTR_6g034485 60.317 126 46 2 123 247 2 124 2.68e-47 157
MS.gene016532.t1 MTR_7g026770 49.215 191 74 2 77 266 133 301 1.17e-46 162
MS.gene016532.t1 MTR_7g026850 64.286 112 40 0 7 118 55 166 1.18e-44 152
MS.gene016532.t1 MTR_6g034495 65.041 123 37 3 3 119 45 167 3.49e-44 151
MS.gene016532.t1 MTR_4g094825 53.425 146 57 1 221 366 252 386 4.98e-44 157
MS.gene016532.t1 MTR_4g094825 52.273 132 58 2 6 133 139 269 3.75e-33 128
MS.gene016532.t1 MTR_3g102030 32.099 324 182 9 8 325 29 320 8.88e-42 149
MS.gene016532.t1 MTR_6g045553 30.484 351 207 8 52 368 125 472 4.79e-38 143
MS.gene016532.t1 MTR_7g026830 65.882 85 29 0 17 101 14 98 7.99e-32 116
MS.gene016532.t1 MTR_7g026800 38.953 172 69 4 152 316 31 173 7.91e-25 100
MS.gene016532.t1 MTR_4g007540 24.728 368 251 13 9 362 73 428 2.26e-24 104
MS.gene016532.t1 MTR_8g075610 27.348 362 231 14 15 360 68 413 5.26e-24 103
MS.gene016532.t1 MTR_4g022780 36.842 171 73 3 152 316 32 173 1.57e-23 96.7
MS.gene016532.t1 MTR_2g064495 26.257 358 239 13 9 353 73 418 4.70e-23 100
MS.gene016532.t1 MTR_2g105330 26.257 358 239 13 9 353 73 418 5.97e-23 100
MS.gene016532.t1 MTR_3g098980 26.393 341 227 12 22 352 92 418 8.07e-23 99.8
MS.gene016532.t1 MTR_8g096530 26.518 313 207 10 21 323 86 385 2.78e-22 98.2
MS.gene016532.t1 MTR_7g026780 47.619 105 29 2 260 364 26 104 1.26e-20 86.7
MS.gene016532.t1 MTR_3g026950 25.776 322 189 11 72 356 129 437 3.50e-19 89.4
MS.gene016532.t1 MTR_3g049150 24.756 307 206 8 72 356 130 433 4.70e-18 85.9
MS.gene016532.t1 MTR_4g055170 28.000 275 165 11 67 321 141 402 6.66e-18 85.5
MS.gene016532.t1 MTR_7g026870 66.667 54 18 0 226 279 98 151 2.05e-16 76.3
MS.gene016532.t1 MTR_4g013050 23.416 363 213 15 24 362 83 404 3.96e-15 76.6
MS.gene016532.t1 MTR_7g084940 26.807 332 203 12 1 320 89 392 9.71e-15 75.9
MS.gene016532.t1 MTR_1g081660 23.684 342 192 12 12 321 92 396 1.38e-14 75.5
MS.gene016532.t1 MTR_1g040195 25.410 366 215 17 22 358 69 405 4.53e-14 73.6
MS.gene016532.t1 MTR_2g015430 25.271 277 164 12 67 322 131 385 7.69e-14 73.2
MS.gene016532.t1 MTR_3g465980 24.662 296 182 12 10 290 86 355 1.24e-12 69.3
MS.gene016532.t1 MTR_4g097090 21.978 364 253 12 13 352 84 440 1.35e-12 69.3
MS.gene016532.t1 MTR_3g049140 23.932 234 153 7 139 356 6 230 1.41e-12 67.4
MS.gene016532.t1 MTR_6g015320 26.087 161 111 3 163 320 251 406 8.04e-12 67.0
MS.gene016532.t1 MTR_7g056087 25.094 267 176 7 3 252 71 330 1.17e-11 66.2
MS.gene016532.t1 MTR_3g465990 24.373 279 178 12 10 274 86 345 2.55e-11 65.5
MS.gene016532.t1 MTR_6g015315 25.121 207 144 6 160 361 245 445 2.91e-11 65.1
MS.gene016532.t1 MTR_7g084910 26.038 265 170 8 2 254 82 332 3.32e-11 65.1
MS.gene016532.t1 MTR_7g026880 54.545 44 20 0 323 366 5 48 7.08e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016532.t1 AT5G67150 36.729 373 221 8 3 366 75 441 7.92e-76 242
MS.gene016532.t1 AT3G50300 38.710 372 208 9 8 370 78 438 8.59e-74 237
MS.gene016532.t1 AT3G50270 38.261 345 205 6 14 352 87 429 6.74e-71 229
MS.gene016532.t1 AT3G50280 35.695 367 220 7 9 366 79 438 6.49e-69 224
MS.gene016532.t1 AT5G42830 33.425 365 230 7 10 366 85 444 1.92e-66 218
MS.gene016532.t1 AT5G07850 35.027 374 231 8 7 373 87 455 1.01e-64 213
MS.gene016532.t1 AT5G67160 36.957 368 213 10 7 367 73 428 1.91e-64 212
MS.gene016532.t1 AT5G07860 35.326 368 218 10 7 366 89 444 2.97e-64 212
MS.gene016532.t1 AT5G38130 36.364 363 205 8 3 352 93 442 1.47e-63 210
MS.gene016532.t1 AT5G07870 34.574 376 230 9 11 373 92 464 2.19e-60 202
MS.gene016532.t1 AT5G01210 32.031 384 215 8 21 366 89 464 5.46e-60 201
MS.gene016532.t1 AT2G39980 31.818 396 209 9 22 366 90 475 3.83e-58 197
MS.gene016532.t1 AT3G50280 35.144 313 192 5 60 366 1 308 3.63e-55 184
MS.gene016532.t1 AT5G23940 32.386 352 217 10 28 366 104 447 2.88e-46 165
MS.gene016532.t1 AT3G50290 38.828 273 149 8 57 318 4 269 5.91e-45 156
MS.gene016532.t1 AT5G48930 26.331 357 231 10 14 352 76 418 7.51e-24 102
MS.gene016532.t1 AT3G48720 27.401 354 224 13 22 359 87 423 1.20e-21 96.3
MS.gene016532.t1 AT2G40230 24.922 321 224 8 9 321 76 387 4.66e-20 91.7
MS.gene016532.t1 AT5G41040 24.719 356 235 12 21 360 96 434 2.33e-19 89.7
MS.gene016532.t1 AT5G41040 24.719 356 235 12 21 360 112 450 2.85e-19 89.7
MS.gene016532.t1 AT1G28680 25.368 272 167 11 67 321 135 387 2.98e-15 77.4
MS.gene016532.t1 AT5G63560 27.778 306 179 14 72 360 140 420 2.18e-14 74.7
MS.gene016532.t1 AT5G39090 28.086 324 194 16 59 359 129 436 9.80e-14 72.8
MS.gene016532.t1 AT1G28680 26.364 220 145 8 67 277 135 346 1.85e-13 71.6
MS.gene016532.t1 AT1G65450 24.405 336 218 13 2 320 84 400 2.84e-13 71.2
MS.gene016532.t1 AT1G27620 25.641 312 206 11 22 321 87 384 2.03e-12 68.6
MS.gene016532.t1 AT5G57840 23.380 355 232 14 22 352 84 422 3.83e-12 67.8
MS.gene016532.t1 AT3G29670 21.467 368 254 10 8 352 78 433 4.47e-12 67.8
MS.gene016532.t1 AT2G19070 21.409 369 249 14 22 360 86 443 2.74e-11 65.5
MS.gene016532.t1 AT3G47170 27.979 193 122 8 74 250 153 344 6.40e-11 64.3

Find 95 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTATCATATGATTATTT+TGG 0.100062 8.2:-59893009 MS.gene016532:CDS
TGATATTCTCCTTTGTAAAA+TGG 0.189048 8.2:+59893199 None:intergenic
GTGTCTTCCATACAAGATTT+TGG 0.229588 8.2:-59892641 MS.gene016532:CDS
TTCTTGTGGGTCAAGTTGTT+TGG 0.295626 8.2:+59893080 None:intergenic
CACTCCTTCTTTCCACTTAA+CGG 0.298603 8.2:-59893528 MS.gene016532:CDS
AAGCACTTTGCACCCTTCTT+TGG 0.311353 8.2:-59893119 MS.gene016532:CDS
TGGAAAGAAGGAGTGGAAAA+TGG 0.312898 8.2:+59893536 None:intergenic
GGTATTTATGATATAGATAT+TGG 0.318666 8.2:-59892766 MS.gene016532:CDS
CTATCAGCCCTTACAAAGTT+TGG 0.338402 8.2:+59892835 None:intergenic
CCAAAATAATCATATGATAA+TGG 0.339734 8.2:+59893009 None:intergenic
TTCGCATTTCATCAATTCAT+TGG 0.367434 8.2:-59893388 MS.gene016532:CDS
CAAGAACACCAAACTTTGTA+AGG 0.370885 8.2:-59892843 MS.gene016532:CDS
TTTACAATGCAGTCGGTTGT+AGG 0.384341 8.2:-59892545 None:intergenic
CAAAATTCACTTCTTCTTGT+GGG 0.396444 8.2:+59893067 None:intergenic
ATGGGACGTTGAATTCCATT+TGG 0.398767 8.2:+59893303 None:intergenic
CGTCAATTAACTCTGTTACT+TGG 0.401148 8.2:+59893463 None:intergenic
TATCATAAATACCGAACAAT+GGG 0.404104 8.2:+59892776 None:intergenic
CTTGAACGTTTATTTCCAAA+TGG 0.404306 8.2:-59893318 MS.gene016532:CDS
CTATTAGAAGAAGGTGGAAT+TGG 0.408965 8.2:-59892943 MS.gene016532:CDS
TTATGATATAGATATTGGTT+GGG 0.419233 8.2:-59892761 MS.gene016532:CDS
TTGGAGGTACATATATTTGA+TGG 0.421304 8.2:+59893343 None:intergenic
GTAGTTCAAATAAAAGTAAT+GGG 0.426060 8.2:-59892714 MS.gene016532:CDS
AAATGGAATATTCTCTCAGT+TGG 0.431761 8.2:+59893216 None:intergenic
TTTATGATATAGATATTGGT+TGG 0.437377 8.2:-59892762 MS.gene016532:CDS
GGGAAAATTAGTGTGTTTGC+TGG 0.438194 8.2:-59892694 MS.gene016532:CDS
TGTGGTGTCTTTATTATGAA+AGG 0.442379 8.2:+59893273 None:intergenic
ATCAGTGGTTCCCCATTGTT+CGG 0.444591 8.2:-59892787 MS.gene016532:CDS
ACATGTGTTGCCGACATCCT+TGG 0.445731 8.2:-59893576 MS.gene016532:CDS
ATGACCTCCAAAATCTTGTA+TGG 0.449025 8.2:+59892634 None:intergenic
TAGTTTATAACTCCGTTAAG+TGG 0.458930 8.2:+59893516 None:intergenic
GGAAATAAACGTTCAAGTGT+TGG 0.459102 8.2:+59893324 None:intergenic
TATGTTTAAGTGTTAGATCA+AGG 0.463006 8.2:-59893041 MS.gene016532:CDS
ACAAAATTCACTTCTTCTTG+TGG 0.465083 8.2:+59893066 None:intergenic
GATGACCAAGGAATTACTAT+TGG 0.466288 8.2:+59892807 None:intergenic
GGTAGTTCAAATAAAAGTAA+TGG 0.470613 8.2:-59892715 MS.gene016532:CDS
ATGAACCATGTTGTCAATGA+TGG 0.476764 8.2:-59893420 MS.gene016532:CDS
GTTGGTACCGGCCTCTACAT+TGG 0.477478 8.2:+59893158 None:intergenic
ACATGATCGATGCATCTACT+TGG 0.480123 8.2:-59892591 MS.gene016532:CDS
TCAGAACGGATCTTAATTTG+TGG 0.481803 8.2:+59893255 None:intergenic
TATGATATAGATATTGGTTG+GGG 0.489973 8.2:-59892760 MS.gene016532:CDS
GGAAATGCTATGATAGTTTG+TGG 0.496006 8.2:-59892988 MS.gene016532:CDS
TTTAAGTGTTAGATCAAGGT+TGG 0.497707 8.2:-59893037 MS.gene016532:CDS
AGGAGTGGAAAATGGAAGGA+AGG 0.501633 8.2:+59893544 None:intergenic
TAAAGAGTCAATATGAGTCT+TGG 0.503318 8.2:-59892867 MS.gene016532:CDS
AACTCCGTTAAGTGGAAAGA+AGG 0.506423 8.2:+59893524 None:intergenic
AACCGACTGCATTGTAAAGA+AGG 0.508715 8.2:+59892550 None:intergenic
TATTATGAAAGGAAATCGTA+TGG 0.509141 8.2:+59893284 None:intergenic
GAAATGCTATGATAGTTTGT+GGG 0.509687 8.2:-59892987 MS.gene016532:CDS
CAAGTAACAGAGTTAATTGA+CGG 0.510134 8.2:-59893462 MS.gene016532:CDS
AATGGAATATTCTCTCAGTT+GGG 0.515645 8.2:+59893217 None:intergenic
TGTGGCCAATAGTAATTCCT+TGG 0.532331 8.2:-59892812 MS.gene016532:CDS
GTGGGTCAAGTTGTTTGGAA+CGG 0.534920 8.2:+59893085 None:intergenic
AAGGGTGCAAAGTGCTTGCA+TGG 0.541762 8.2:+59893125 None:intergenic
CTACCACTTCGAAATGCCAC+TGG 0.542063 8.2:+59892733 None:intergenic
GAAGAAGGTGGAATTGGTAA+AGG 0.545763 8.2:-59892937 MS.gene016532:CDS
AACGGAGTTATAAACTATGA+AGG 0.549323 8.2:-59893510 MS.gene016532:CDS
GCATCGATCATGTTTAACTC+GGG 0.549955 8.2:+59892601 None:intergenic
GGATCACTGAGCGCCAAAGA+AGG 0.552302 8.2:+59893106 None:intergenic
GTCAAGGAGCTATTAGAAGA+AGG 0.552540 8.2:-59892952 MS.gene016532:CDS
CTGCATTGTAAAGAAGGTTG+TGG 0.554258 8.2:+59892556 None:intergenic
AACCTTCTTTACAATGCAGT+CGG 0.562989 8.2:-59892552 MS.gene016532:CDS
TTAAAAGCCAATGTAGAGGC+CGG 0.565401 8.2:-59893165 MS.gene016532:CDS
TGCATCGATCATGTTTAACT+CGG 0.566663 8.2:+59892600 None:intergenic
AAGAAGGAGTGGAAAATGGA+AGG 0.568464 8.2:+59893540 None:intergenic
CTTTGTAAGGGCTGATAGTG+TGG 0.578499 8.2:-59892830 MS.gene016532:CDS
TCCATCGCATGCAACAATGC+CGG 0.578735 8.2:-59893630 MS.gene016532:CDS
ACCGGCATTGTTGCATGCGA+TGG 0.578867 8.2:+59893629 None:intergenic
GATCACTGAGCGCCAAAGAA+GGG 0.585468 8.2:+59893107 None:intergenic
AAGGAAGGTACGTGGATCCA+AGG 0.590908 8.2:+59893559 None:intergenic
AAATAATCATATGATAATGG+TGG 0.591941 8.2:+59893012 None:intergenic
AGTAATTCCTTGGTCATCAG+TGG 0.592810 8.2:-59892802 MS.gene016532:CDS
ATTATGAAAGGAAATCGTAT+GGG 0.592939 8.2:+59893285 None:intergenic
ATATCATAAATACCGAACAA+TGG 0.600620 8.2:+59892775 None:intergenic
TGGGGTTACAATGAAAGTCA+AGG 0.605492 8.2:-59892968 MS.gene016532:CDS
AAGGAGCTATTAGAAGAAGG+TGG 0.610681 8.2:-59892949 MS.gene016532:CDS
TAAGTTGTTCTTCATCAGAA+CGG 0.613567 8.2:+59893241 None:intergenic
AAAATTAAAAGCCAATGTAG+AGG 0.618767 8.2:-59893169 MS.gene016532:CDS
GTTACTTGGAATGCAAGCAA+TGG 0.622375 8.2:+59893477 None:intergenic
CGTTAAGTGGAAAGAAGGAG+TGG 0.624290 8.2:+59893529 None:intergenic
ACATTCCATCATTGACAACA+TGG 0.630015 8.2:+59893415 None:intergenic
AATAAACGTTCAAGTGTTGG+AGG 0.630066 8.2:+59893327 None:intergenic
GTACGTGGATCCAAGGATGT+CGG 0.638137 8.2:+59893566 None:intergenic
TGAATTGATGAAATGCGAAG+TGG 0.638559 8.2:+59893392 None:intergenic
TGCATGAACAAAGAGTGCAC+CGG 0.640901 8.2:+59893611 None:intergenic
AAGAAGGTTGTGGAGGATCG+AGG 0.642827 8.2:+59892566 None:intergenic
CATTGTAAAGAAGGTTGTGG+AGG 0.644905 8.2:+59892559 None:intergenic
TGGGGAACCACTGATGACCA+AGG 0.650837 8.2:+59892795 None:intergenic
AAGAACACCAAACTTTGTAA+GGG 0.654921 8.2:-59892842 MS.gene016532:CDS
AAATGCTATGATAGTTTGTG+GGG 0.661468 8.2:-59892986 MS.gene016532:CDS
CATCGATCATGTTTAACTCG+GGG 0.666256 8.2:+59892602 None:intergenic
AATCCAGTGGCATTTCGAAG+TGG 0.670575 8.2:-59892736 MS.gene016532:CDS
TATTGGTTGGGGAAATCCAG+TGG 0.672210 8.2:-59892749 MS.gene016532:CDS
ATCATAAATACCGAACAATG+GGG 0.684937 8.2:+59892777 None:intergenic
GAAAATGGAAGGAAGGTACG+TGG 0.689733 8.2:+59893551 None:intergenic
TCATTGGCTGAAATCACACG+TGG 0.724412 8.2:-59893372 MS.gene016532:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCAAAATAATCATATGATAA+TGG + chr8.2:59893633-59893652 None:intergenic 20.0%
!! GGTATTTATGATATAGATAT+TGG - chr8.2:59893873-59893892 MS.gene016532:intron 20.0%
!! GTAGTTCAAATAAAAGTAAT+GGG - chr8.2:59893925-59893944 MS.gene016532:intron 20.0%
!! TTTATGATATAGATATTGGT+TGG - chr8.2:59893877-59893896 MS.gene016532:intron 20.0%
!!! AAATAATCATATGATAATGG+TGG + chr8.2:59893630-59893649 None:intergenic 20.0%
!!! CCATTATCATATGATTATTT+TGG - chr8.2:59893630-59893649 MS.gene016532:CDS 20.0%
!!! TTATGATATAGATATTGGTT+GGG - chr8.2:59893878-59893897 MS.gene016532:intron 20.0%
! AAAATTAAAAGCCAATGTAG+AGG - chr8.2:59893470-59893489 MS.gene016532:CDS 25.0%
! ATATCATAAATACCGAACAA+TGG + chr8.2:59893867-59893886 None:intergenic 25.0%
! ATTATGAAAGGAAATCGTAT+GGG + chr8.2:59893357-59893376 None:intergenic 25.0%
! GGTAGTTCAAATAAAAGTAA+TGG - chr8.2:59893924-59893943 MS.gene016532:intron 25.0%
! TATCATAAATACCGAACAAT+GGG + chr8.2:59893866-59893885 None:intergenic 25.0%
! TATGTTTAAGTGTTAGATCA+AGG - chr8.2:59893598-59893617 MS.gene016532:CDS 25.0%
! TATTATGAAAGGAAATCGTA+TGG + chr8.2:59893358-59893377 None:intergenic 25.0%
! TGATATTCTCCTTTGTAAAA+TGG + chr8.2:59893443-59893462 None:intergenic 25.0%
!! GAGAATATTCCATTTTACAA+AGG - chr8.2:59893431-59893450 MS.gene016532:CDS 25.0%
!! TATGATATAGATATTGGTTG+GGG - chr8.2:59893879-59893898 MS.gene016532:intron 25.0%
!!! TTTAAAGTGTTGGATTTTGT+TGG + chr8.2:59892902-59892921 None:intergenic 25.0%
AAATGGAATATTCTCTCAGT+TGG + chr8.2:59893426-59893445 None:intergenic 30.0%
AACGGAGTTATAAACTATGA+AGG - chr8.2:59893129-59893148 MS.gene016532:CDS 30.0%
AAGAACACCAAACTTTGTAA+GGG - chr8.2:59893797-59893816 MS.gene016532:intron 30.0%
AATGGAATATTCTCTCAGTT+GGG + chr8.2:59893425-59893444 None:intergenic 30.0%
ACAAAATTCACTTCTTCTTG+TGG + chr8.2:59893576-59893595 None:intergenic 30.0%
ATCATAAATACCGAACAATG+GGG + chr8.2:59893865-59893884 None:intergenic 30.0%
CAAAATTCACTTCTTCTTGT+GGG + chr8.2:59893575-59893594 None:intergenic 30.0%
CAAGTAACAGAGTTAATTGA+CGG - chr8.2:59893177-59893196 MS.gene016532:CDS 30.0%
CTTGAACGTTTATTTCCAAA+TGG - chr8.2:59893321-59893340 MS.gene016532:CDS 30.0%
TAAAATTGAGTTAACACCGT+GGG - chr8.2:59892804-59892823 MS.gene016532:CDS 30.0%
TAAAGAGTCAATATGAGTCT+TGG - chr8.2:59893772-59893791 MS.gene016532:intron 30.0%
TAAGTTGTTCTTCATCAGAA+CGG + chr8.2:59893401-59893420 None:intergenic 30.0%
TAGTTTATAACTCCGTTAAG+TGG + chr8.2:59893126-59893145 None:intergenic 30.0%
TGGTAAAAAAGAAGGAAGTA+TGG - chr8.2:59893965-59893984 MS.gene016532:intron 30.0%
TGTGGTGTCTTTATTATGAA+AGG + chr8.2:59893369-59893388 None:intergenic 30.0%
TTCGCATTTCATCAATTCAT+TGG - chr8.2:59893251-59893270 MS.gene016532:CDS 30.0%
TTTAAGTGTTAGATCAAGGT+TGG - chr8.2:59893602-59893621 MS.gene016532:CDS 30.0%
! AAAGAGACTGTTTAAAGTGT+TGG + chr8.2:59892912-59892931 None:intergenic 30.0%
! AAATGCTATGATAGTTTGTG+GGG - chr8.2:59893653-59893672 MS.gene016532:CDS 30.0%
! GAAATGCTATGATAGTTTGT+GGG - chr8.2:59893652-59893671 MS.gene016532:CDS 30.0%
! TTGCATGGAAGATATTTTGT+TGG + chr8.2:59893502-59893521 None:intergenic 30.0%
! TTGGAGGTACATATATTTGA+TGG + chr8.2:59893299-59893318 None:intergenic 30.0%
AACCTTCTTTACAATGCAGT+CGG - chr8.2:59894087-59894106 MS.gene016532:intron 35.0%
ACATTCCATCATTGACAACA+TGG + chr8.2:59893227-59893246 None:intergenic 35.0%
ATGAACCATGTTGTCAATGA+TGG - chr8.2:59893219-59893238 MS.gene016532:CDS 35.0%
ATGACCTCCAAAATCTTGTA+TGG + chr8.2:59894008-59894027 None:intergenic 35.0%
CAAGAACACCAAACTTTGTA+AGG - chr8.2:59893796-59893815 MS.gene016532:intron 35.0%
CGTCAATTAACTCTGTTACT+TGG + chr8.2:59893179-59893198 None:intergenic 35.0%
CTATTAGAAGAAGGTGGAAT+TGG - chr8.2:59893696-59893715 MS.gene016532:intron 35.0%
GATGACCAAGGAATTACTAT+TGG + chr8.2:59893835-59893854 None:intergenic 35.0%
GGAAATAAACGTTCAAGTGT+TGG + chr8.2:59893318-59893337 None:intergenic 35.0%
GGAAATGCTATGATAGTTTG+TGG - chr8.2:59893651-59893670 MS.gene016532:CDS 35.0%
GTAAAATTGAGTTAACACCG+TGG - chr8.2:59892803-59892822 MS.gene016532:CDS 35.0%
TCTCTCTACCACTACTATTA+AGG - chr8.2:59892753-59892772 MS.gene016532:CDS 35.0%
TGAATTGATGAAATGCGAAG+TGG + chr8.2:59893250-59893269 None:intergenic 35.0%
TGGAAAGAATGCAAGAACAT+GGG + chr8.2:59892935-59892954 None:intergenic 35.0%
TTAGTTGAGTCACCTACATT+TGG + chr8.2:59892781-59892800 None:intergenic 35.0%
! ACTATTAAGGCACCAAATGT+AGG - chr8.2:59892766-59892785 MS.gene016532:CDS 35.0%
! ATAAAGAAACCCCTTTTGGT+TGG + chr8.2:59892854-59892873 None:intergenic 35.0%
! GGAAGATATTTTGTTGGTAC+CGG + chr8.2:59893496-59893515 None:intergenic 35.0%
! GTGTCTTCCATACAAGATTT+TGG - chr8.2:59893998-59894017 MS.gene016532:intron 35.0%
! GTGTTTGCTGGTAAAAAAGA+AGG - chr8.2:59893957-59893976 MS.gene016532:intron 35.0%
! TCAGAACGGATCTTAATTTG+TGG + chr8.2:59893387-59893406 None:intergenic 35.0%
! TCTTCCATACAAGATTTTGG+AGG - chr8.2:59894001-59894020 MS.gene016532:intron 35.0%
! TGCATCGATCATGTTTAACT+CGG + chr8.2:59894042-59894061 None:intergenic 35.0%
! TTGACGGTACTTTTGTTAGT+TGG - chr8.2:59893193-59893212 MS.gene016532:CDS 35.0%
!! AATAAACGTTCAAGTGTTGG+AGG + chr8.2:59893315-59893334 None:intergenic 35.0%
!! ATACAAGATTTTGGAGGTCA+TGG - chr8.2:59894007-59894026 MS.gene016532:intron 35.0%
!! ATTTGGTGCCTTAATAGTAG+TGG + chr8.2:59892764-59892783 None:intergenic 35.0%
!! TACAAGATTTTGGAGGTCAT+GGG - chr8.2:59894008-59894027 MS.gene016532:intron 35.0%
!! TCGAAAAGCACTCATATTTG+AGG + chr8.2:59892731-59892750 None:intergenic 35.0%
AACCGACTGCATTGTAAAGA+AGG + chr8.2:59894092-59894111 None:intergenic 40.0%
AACTCCGTTAAGTGGAAAGA+AGG + chr8.2:59893118-59893137 None:intergenic 40.0%
AAGAAGGAGTGGAAAATGGA+AGG + chr8.2:59893102-59893121 None:intergenic 40.0%
AAGGAGCTATTAGAAGAAGG+TGG - chr8.2:59893690-59893709 MS.gene016532:intron 40.0%
ACATGATCGATGCATCTACT+TGG - chr8.2:59894048-59894067 MS.gene016532:intron 40.0%
AGTAATTCCTTGGTCATCAG+TGG - chr8.2:59893837-59893856 MS.gene016532:intron 40.0%
ATGGGACGTTGAATTCCATT+TGG + chr8.2:59893339-59893358 None:intergenic 40.0%
CACAACAAGGATCTTGTGTA+AGG - chr8.2:59892685-59892704 MS.gene016532:CDS 40.0%
CACTCCTTCTTTCCACTTAA+CGG - chr8.2:59893111-59893130 MS.gene016532:CDS 40.0%
CATCGATCATGTTTAACTCG+GGG + chr8.2:59894040-59894059 None:intergenic 40.0%
CATTGTAAAGAAGGTTGTGG+AGG + chr8.2:59894083-59894102 None:intergenic 40.0%
CTATCAGCCCTTACAAAGTT+TGG + chr8.2:59893807-59893826 None:intergenic 40.0%
CTGCATTGTAAAGAAGGTTG+TGG + chr8.2:59894086-59894105 None:intergenic 40.0%
GAAGAAGGTGGAATTGGTAA+AGG - chr8.2:59893702-59893721 MS.gene016532:intron 40.0%
GGGAAAATTAGTGTGTTTGC+TGG - chr8.2:59893945-59893964 MS.gene016532:intron 40.0%
GTCAAGGAGCTATTAGAAGA+AGG - chr8.2:59893687-59893706 MS.gene016532:intron 40.0%
GTGGAAAGAATGCAAGAACA+TGG + chr8.2:59892936-59892955 None:intergenic 40.0%
GTTACTTGGAATGCAAGCAA+TGG + chr8.2:59893165-59893184 None:intergenic 40.0%
GTTTCTTTATCACCATCCCA+CGG - chr8.2:59892864-59892883 MS.gene016532:CDS 40.0%
TGCACATTCTCATCACAACA+AGG - chr8.2:59892672-59892691 MS.gene016532:CDS 40.0%
TGGAAAGAAGGAGTGGAAAA+TGG + chr8.2:59893106-59893125 None:intergenic 40.0%
TGGGGTTACAATGAAAGTCA+AGG - chr8.2:59893671-59893690 MS.gene016532:CDS 40.0%
TGTGGCCAATAGTAATTCCT+TGG - chr8.2:59893827-59893846 MS.gene016532:intron 40.0%
TTAAAAGCCAATGTAGAGGC+CGG - chr8.2:59893474-59893493 MS.gene016532:CDS 40.0%
! GCATCGATCATGTTTAACTC+GGG + chr8.2:59894041-59894060 None:intergenic 40.0%
! GGTGATAAAGAAACCCCTTT+TGG + chr8.2:59892858-59892877 None:intergenic 40.0%
! TTCTTGTGGGTCAAGTTGTT+TGG + chr8.2:59893562-59893581 None:intergenic 40.0%
AAGCACTTTGCACCCTTCTT+TGG - chr8.2:59893520-59893539 MS.gene016532:CDS 45.0%
AATCCAGTGGCATTTCGAAG+TGG - chr8.2:59893903-59893922 MS.gene016532:intron 45.0%
AGGAGTGGAAAATGGAAGGA+AGG + chr8.2:59893098-59893117 None:intergenic 45.0%
ATCAGTGGTTCCCCATTGTT+CGG - chr8.2:59893852-59893871 MS.gene016532:intron 45.0%
ATTTCGAGACGACCTGCAAA+GGG + chr8.2:59892958-59892977 None:intergenic 45.0%
CCGTTTGGATCCAACCAAAA+GGG - chr8.2:59892841-59892860 MS.gene016532:CDS 45.0%
CGTTAAGTGGAAAGAAGGAG+TGG + chr8.2:59893113-59893132 None:intergenic 45.0%
CGTTTGGATCCAACCAAAAG+GGG - chr8.2:59892842-59892861 MS.gene016532:CDS 45.0%
TATTTCGAGACGACCTGCAA+AGG + chr8.2:59892959-59892978 None:intergenic 45.0%
! CTTTGTAAGGGCTGATAGTG+TGG - chr8.2:59893809-59893828 MS.gene016532:intron 45.0%
! GAAAATGGAAGGAAGGTACG+TGG + chr8.2:59893091-59893110 None:intergenic 45.0%
! GTGGGTCAAGTTGTTTGGAA+CGG + chr8.2:59893557-59893576 None:intergenic 45.0%
! TATTGGTTGGGGAAATCCAG+TGG - chr8.2:59893890-59893909 MS.gene016532:intron 45.0%
! TCATTGGCTGAAATCACACG+TGG - chr8.2:59893267-59893286 MS.gene016532:CDS 45.0%
! TGCATGAACAAAGAGTGCAC+CGG + chr8.2:59893031-59893050 None:intergenic 45.0%
!! CCCTTTTGGTTGGATCCAAA+CGG + chr8.2:59892844-59892863 None:intergenic 45.0%
!! CCTTTTGGTTGGATCCAAAC+GGG + chr8.2:59892843-59892862 None:intergenic 45.0%
AAGAAGGTTGTGGAGGATCG+AGG + chr8.2:59894076-59894095 None:intergenic 50.0%
ACATGTGTTGCCGACATCCT+TGG - chr8.2:59893063-59893082 MS.gene016532:CDS 50.0%
CCCGTTTGGATCCAACCAAA+AGG - chr8.2:59892840-59892859 MS.gene016532:CDS 50.0%
CTACCACTTCGAAATGCCAC+TGG + chr8.2:59893909-59893928 None:intergenic 50.0%
GATCACTGAGCGCCAAAGAA+GGG + chr8.2:59893535-59893554 None:intergenic 50.0%
GATCTTCAGTTCCTCCCGTT+TGG - chr8.2:59892826-59892845 MS.gene016532:CDS 50.0%
GCATTCTTTCCACCCTTTGC+AGG - chr8.2:59892943-59892962 MS.gene016532:CDS 50.0%
GTACGTGGATCCAAGGATGT+CGG + chr8.2:59893076-59893095 None:intergenic 50.0%
TCCATCGCATGCAACAATGC+CGG - chr8.2:59893009-59893028 MS.gene016532:CDS 50.0%
TTGGACGTGTTCACTACCGT+GGG + chr8.2:59892883-59892902 None:intergenic 50.0%
TTTGGTTGGATCCAAACGGG+AGG + chr8.2:59892840-59892859 None:intergenic 50.0%
! AAGGAAGGTACGTGGATCCA+AGG + chr8.2:59893083-59893102 None:intergenic 50.0%
! AAGGGTGCAAAGTGCTTGCA+TGG + chr8.2:59893517-59893536 None:intergenic 50.0%
ACCGGCATTGTTGCATGCGA+TGG + chr8.2:59893013-59893032 None:intergenic 55.0%
ACGTGTTCACTACCGTGGGA+TGG + chr8.2:59892879-59892898 None:intergenic 55.0%
GGATCACTGAGCGCCAAAGA+AGG + chr8.2:59893536-59893555 None:intergenic 55.0%
GGGAGGAACTGAAGATCCCA+CGG + chr8.2:59892823-59892842 None:intergenic 55.0%
GTTGGACGTGTTCACTACCG+TGG + chr8.2:59892884-59892903 None:intergenic 55.0%
TGGGGAACCACTGATGACCA+AGG + chr8.2:59893847-59893866 None:intergenic 55.0%
!! GTTGGTACCGGCCTCTACAT+TGG + chr8.2:59893484-59893503 None:intergenic 55.0%
TCGAGACGACCTGCAAAGGG+TGG + chr8.2:59892955-59892974 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 59892546 59894115 59892546 ID=MS.gene016532
chr8.2 mRNA 59892546 59894115 59892546 ID=MS.gene016532.t1;Parent=MS.gene016532
chr8.2 exon 59894100 59894115 59894100 ID=MS.gene016532.t1.exon1;Parent=MS.gene016532.t1
chr8.2 CDS 59894100 59894115 59894100 ID=cds.MS.gene016532.t1;Parent=MS.gene016532.t1
chr8.2 exon 59892546 59893696 59892546 ID=MS.gene016532.t1.exon2;Parent=MS.gene016532.t1
chr8.2 CDS 59892546 59893696 59892546 ID=cds.MS.gene016532.t1;Parent=MS.gene016532.t1
Gene Sequence

>MS.gene016532

ATGCAATTTAGCACAAGTACGTATATTTTTTATTTTTAACAAAACTTACTTTTACTTTATCAATGAAAGCACATATTTTTTTTGAATTCGTTGAGAGAAAATCTCTATATAAATTGTAATAACTTGTGCACATTCTCATCACAACAAGGATCTTGTGTAAGGCTTAGCTGAACACTACGTTACCTCAAATATGAGTGCTTTTCGAGTTCTCTCTACCACTACTATTAAGGCACCAAATGTAGGTGACTCAACTAATCGTAAAATTGAGTTAACACCGTGGGATCTTCAGTTCCTCCCGTTTGGATCCAACCAAAAGGGGTTTCTTTATCACCATCCCACGGTAGTGAACACGTCCAACAAAATCCAACACTTTAAACAGTCTCTTTCCCATGTTCTTGCATTCTTTCCACCCTTTGCAGGTCGTCTCGAAATAACTAAACATGAAGATAACACTATCTCGTGTTCCATCGCATGCAACAATGCCGGTGCACTCTTTGTTCATGCAAAAGCAGAGAATACATGTGTTGCCGACATCCTTGGATCCACGTACCTTCCTTCCATTTTCCACTCCTTCTTTCCACTTAACGGAGTTATAAACTATGAAGGTACATCACATCCATTGCTTGCATTCCAAGTAACAGAGTTAATTGACGGTACTTTTGTTAGTTGGACAATGAACCATGTTGTCAATGATGGAATGTCCACTTCGCATTTCATCAATTCATTGGCTGAAATCACACGTGGTTCTTGCCATCAAATATATGTACCTCCAACACTTGAACGTTTATTTCCAAATGGAATTCAACGTCCCATACGATTTCCTTTCATAATAAAGACACCACAAATTAAGATCCGTTCTGATGAAGAACAACTTATCCCAACTGAGAGAATATTCCATTTTACAAAGGAGAATATCATGAGAATAAAATTAAAAGCCAATGTAGAGGCCGGTACCAACAAAATATCTTCCATGCAAGCACTTTGCACCCTTCTTTGGCGCTCAGTGATCCGTTCCAAACAACTTGACCCACAAGAAGAAGTGAATTTTGTGTTATGTTTAAGTGTTAGATCAAGGTTGGTTCCACCATTATCATATGATTATTTTGGAAATGCTATGATAGTTTGTGGGGTTACAATGAAAGTCAAGGAGCTATTAGAAGAAGGTGGAATTGGTAAAGGTGCTTTAGAGATGTACAAGATTATTGCTTTGCACTCTGATGAGAAATTAAAGAGTCAATATGAGTCTTGGTCAAGAACACCAAACTTTGTAAGGGCTGATAGTGTGGCCAATAGTAATTCCTTGGTCATCAGTGGTTCCCCATTGTTCGGTATTTATGATATAGATATTGGTTGGGGAAATCCAGTGGCATTTCGAAGTGGTAGTTCAAATAAAAGTAATGGGAAAATTAGTGTGTTTGCTGGTAAAAAAGAAGGAAGTATGGACTTTGAAGTGTGTCTTCCATACAAGATTTTGGAGGTCATGGGAAATGACCCCGAGTTAAACATGATCGATGCATCTACTTGGAACCTCGATCCTCCACAACCTTCTTTACAATGCAGTCGGTTGTAG
Protein sequence

>MS.gene016532.t1

MQFSTSRLEITKHEDNTISCSIACNNAGALFVHAKAENTCVADILGSTYLPSIFHSFFPLNGVINYEGTSHPLLAFQVTELIDGTFVSWTMNHVVNDGMSTSHFINSLAEITRGSCHQIYVPPTLERLFPNGIQRPIRFPFIIKTPQIKIRSDEEQLIPTERIFHFTKENIMRIKLKANVEAGTNKISSMQALCTLLWRSVIRSKQLDPQEEVNFVLCLSVRSRLVPPLSYDYFGNAMIVCGVTMKVKELLEEGGIGKGALEMYKIIALHSDEKLKSQYESWSRTPNFVRADSVANSNSLVISGSPLFGIYDIDIGWGNPVAFRSGSSNKSNGKISVFAGKKEGSMDFEVCLPYKILEVMGNDPELNMIDASTWNLDPPQPSLQCSRL