AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56798

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56798.t1 MTR_4g094835 92.170 447 34 1 1 447 1 446 0.0 855
MS.gene56798.t1 MTR_4g094838 81.778 450 60 5 4 448 1 433 0.0 726
MS.gene56798.t1 MTR_4g094832 86.400 375 47 1 78 448 57 431 0.0 666
MS.gene56798.t1 MTR_4g094842 60.352 454 159 9 1 443 1 444 0.0 538
MS.gene56798.t1 MTR_8g041770 58.259 448 171 7 1 443 1 437 0.0 518
MS.gene56798.t1 MTR_0190s0020 57.238 449 175 8 1 443 1 438 3.60e-177 503
MS.gene56798.t1 MTR_4g022720 56.444 450 170 8 1 443 1 431 6.08e-173 492
MS.gene56798.t1 MTR_8g079710 57.303 445 174 10 1 438 1 436 3.82e-171 488
MS.gene56798.t1 MTR_4g022825 54.725 455 175 9 1 443 1 436 9.09e-170 484
MS.gene56798.t1 MTR_4g094828 54.525 453 181 11 1 439 1 442 1.34e-167 479
MS.gene56798.t1 MTR_3g088420 54.788 449 177 9 1 443 1 429 5.49e-166 474
MS.gene56798.t1 MTR_4g022670 53.509 456 180 8 1 443 1 437 1.38e-165 473
MS.gene56798.t1 MTR_4g022800 54.486 457 175 9 1 443 1 438 3.89e-165 473
MS.gene56798.t1 MTR_4g022290 55.011 449 180 10 1 439 1 437 2.11e-164 471
MS.gene56798.t1 MTR_3g026310 52.484 463 181 9 1 443 1 444 1.86e-162 466
MS.gene56798.t1 MTR_7g026860 52.412 456 182 9 1 443 1 434 3.74e-158 454
MS.gene56798.t1 MTR_4g022760 47.588 456 163 8 1 443 1 393 1.01e-135 396
MS.gene56798.t1 MTR_6g016815 41.741 448 171 13 1 443 15 377 4.47e-102 309
MS.gene56798.t1 MTR_3g101900 41.943 422 228 8 32 443 30 444 1.01e-99 306
MS.gene56798.t1 MTR_3g101930 41.014 434 230 9 28 443 26 451 7.02e-99 304
MS.gene56798.t1 MTR_3g102020 38.865 458 248 10 3 443 5 447 6.26e-96 296
MS.gene56798.t1 MTR_3g101960 38.816 456 248 10 3 442 5 445 1.41e-94 293
MS.gene56798.t1 MTR_3g102010 40.930 430 232 8 28 443 26 447 1.74e-94 292
MS.gene56798.t1 MTR_3g101970 39.110 427 231 8 28 443 25 433 1.40e-92 287
MS.gene56798.t1 MTR_4g094825 75.000 192 46 2 19 210 80 269 1.52e-87 272
MS.gene56798.t1 MTR_4g094825 63.314 169 47 2 285 448 234 392 6.72e-66 216
MS.gene56798.t1 MTR_3g070170 35.435 460 251 8 22 447 23 470 6.79e-84 266
MS.gene56798.t1 MTR_8g040780 58.491 212 81 3 234 442 20 227 1.55e-78 244
MS.gene56798.t1 MTR_5g081960 32.869 502 262 9 3 448 5 487 6.11e-78 251
MS.gene56798.t1 MTR_4g015180 33.534 498 255 10 3 443 5 483 5.25e-76 246
MS.gene56798.t1 MTR_8g040760 53.812 223 91 5 1 220 1 214 9.95e-73 228
MS.gene56798.t1 MTR_7g026850 55.769 156 64 2 37 192 12 162 1.93e-56 185
MS.gene56798.t1 MTR_6g045553 29.060 468 264 12 23 445 29 473 1.54e-49 175
MS.gene56798.t1 MTR_3g102030 33.333 303 168 5 103 401 48 320 1.03e-46 164
MS.gene56798.t1 MTR_6g034485 58.915 129 47 2 199 325 1 125 1.85e-45 154
MS.gene56798.t1 MTR_7g026770 47.222 180 71 4 154 331 132 289 9.00e-43 153
MS.gene56798.t1 MTR_6g034495 58.475 118 48 1 75 192 45 161 2.73e-42 147
MS.gene56798.t1 MTR_7g026830 66.667 81 27 0 97 177 16 96 9.87e-33 119
MS.gene56798.t1 MTR_4g007540 25.445 393 251 14 64 436 56 426 1.19e-29 120
MS.gene56798.t1 MTR_3g098980 29.262 393 228 17 57 430 59 420 2.36e-28 116
MS.gene56798.t1 MTR_8g075610 24.936 389 254 14 64 436 47 413 9.88e-28 114
MS.gene56798.t1 MTR_2g064495 23.192 401 265 13 49 428 39 417 1.24e-25 108
MS.gene56798.t1 MTR_2g105330 23.192 401 265 13 49 428 39 417 1.41e-25 108
MS.gene56798.t1 MTR_8g096530 28.011 357 214 13 57 396 52 382 3.91e-24 104
MS.gene56798.t1 MTR_3g026950 25.000 408 249 12 61 432 51 437 7.30e-24 103
MS.gene56798.t1 MTR_4g097090 23.476 443 281 14 27 428 15 440 1.14e-22 100
MS.gene56798.t1 MTR_2g089765 27.984 486 260 24 3 431 15 467 2.38e-22 99.8
MS.gene56798.t1 MTR_8g079970 24.227 388 241 12 49 413 41 398 7.78e-22 97.8
MS.gene56798.t1 MTR_3g049150 24.700 417 265 16 50 432 32 433 1.44e-21 97.1
MS.gene56798.t1 MTR_4g127650 22.921 445 286 14 24 428 13 440 2.54e-21 96.3
MS.gene56798.t1 MTR_3g086020 26.515 396 244 17 62 448 59 416 4.04e-21 95.5
MS.gene56798.t1 MTR_7g014250 24.444 450 272 18 32 438 26 450 1.68e-20 94.0
MS.gene56798.t1 MTR_7g026800 37.805 164 68 6 223 384 29 160 2.63e-20 89.0
MS.gene56798.t1 MTR_7g027175 24.873 394 255 15 57 434 61 429 4.12e-20 92.8
MS.gene56798.t1 MTR_3g028210 24.786 468 282 19 4 428 8 448 8.41e-20 92.0
MS.gene56798.t1 MTR_4g055170 28.053 303 191 10 64 355 69 355 9.51e-20 91.7
MS.gene56798.t1 MTR_8g012750 25.328 458 270 17 29 434 23 460 1.23e-19 91.7
MS.gene56798.t1 MTR_3g071320 25.507 345 220 11 29 352 23 351 1.31e-19 91.3
MS.gene56798.t1 MTR_4g022780 36.747 166 71 6 223 386 30 163 2.30e-19 85.9
MS.gene56798.t1 MTR_1g053315 25.770 357 218 16 64 397 78 410 2.61e-19 90.5
MS.gene56798.t1 MTR_1g081660 23.261 460 270 18 32 447 31 451 3.30e-19 90.1
MS.gene56798.t1 MTR_7g026780 43.519 108 35 2 336 443 26 107 4.04e-19 83.2
MS.gene56798.t1 MTR_6g015315 24.920 313 202 9 151 437 140 445 4.13e-18 86.7
MS.gene56798.t1 MTR_4g013050 23.951 405 239 12 57 438 46 404 4.40e-18 86.3
MS.gene56798.t1 MTR_1g040195 24.163 418 266 15 33 434 23 405 6.43e-18 85.9
MS.gene56798.t1 MTR_7g014230 24.048 420 258 16 30 409 26 424 1.05e-17 85.5
MS.gene56798.t1 MTR_6g015850 25.342 438 276 16 30 436 32 449 1.11e-17 85.5
MS.gene56798.t1 MTR_6g015320 24.889 450 257 17 32 434 29 444 2.35e-17 84.3
MS.gene56798.t1 MTR_7g084940 26.039 361 226 14 52 396 57 392 7.31e-17 82.8
MS.gene56798.t1 MTR_5g084850 23.888 427 254 18 49 447 41 424 8.91e-17 82.4
MS.gene56798.t1 MTR_7g085930 24.627 402 257 13 64 432 59 447 1.41e-16 82.0
MS.gene56798.t1 MTR_6g015815 24.036 441 281 15 30 436 32 452 2.29e-16 81.6
MS.gene56798.t1 MTR_3g087640 26.116 448 274 20 30 434 29 462 3.88e-16 80.9
MS.gene56798.t1 MTR_5g084870 23.885 381 235 15 49 408 41 387 1.11e-15 79.3
MS.gene56798.t1 MTR_2g089650 24.065 428 281 16 30 424 27 443 1.30e-15 79.3
MS.gene56798.t1 MTR_1g040175 22.871 411 272 16 48 436 45 432 1.99e-15 78.6
MS.gene56798.t1 MTR_7g085990 25.622 402 251 15 64 431 55 442 2.88e-15 78.2
MS.gene56798.t1 MTR_7g015860 23.913 414 249 15 58 431 58 445 3.50e-15 77.8
MS.gene56798.t1 MTR_5g026320 23.284 408 257 14 61 442 71 448 6.29e-15 77.0
MS.gene56798.t1 MTR_1g040215 22.829 403 269 13 33 415 23 403 7.46e-15 76.6
MS.gene56798.t1 MTR_7g014200 25.721 451 257 20 30 429 28 451 8.92e-15 76.6
MS.gene56798.t1 MTR_3g049140 24.786 234 154 6 213 432 5 230 1.31e-14 73.9
MS.gene56798.t1 MTR_7g084910 25.240 313 191 9 40 326 36 331 1.57e-14 75.5
MS.gene56798.t1 MTR_7g014610 23.758 463 273 19 22 428 19 457 2.01e-14 75.5
MS.gene56798.t1 MTR_2g007880 24.324 407 253 16 64 446 74 449 2.43e-14 75.1
MS.gene56798.t1 MTR_5g076700 20.320 438 298 13 33 441 24 439 4.01e-14 74.7
MS.gene56798.t1 MTR_7g014160 23.947 451 264 18 32 428 30 455 8.29e-14 73.6
MS.gene56798.t1 MTR_7g114490 22.273 440 272 18 33 434 24 431 1.60e-13 72.8
MS.gene56798.t1 MTR_7g056087 22.573 412 258 17 51 430 41 423 1.71e-13 72.4
MS.gene56798.t1 MTR_7g114520 21.219 443 289 16 33 441 25 441 2.18e-13 72.4
MS.gene56798.t1 MTR_3g465980 24.275 276 187 8 64 329 68 331 6.24e-13 70.9
MS.gene56798.t1 MTR_1g094790 26.341 410 246 19 61 443 57 437 1.54e-12 69.7
MS.gene56798.t1 MTR_7g014620 24.485 437 275 17 21 428 4 414 2.56e-12 68.9
MS.gene56798.t1 MTR_7g026870 50.704 71 28 3 291 355 82 151 3.02e-12 64.7
MS.gene56798.t1 MTR_8g079950 23.294 425 266 17 49 447 41 431 4.18e-12 68.2
MS.gene56798.t1 MTR_7g013850 22.727 396 244 15 58 406 62 442 4.30e-12 68.2
MS.gene56798.t1 MTR_7g114550 20.408 441 293 14 33 441 24 438 5.03e-12 68.2
MS.gene56798.t1 MTR_7g026880 56.000 50 20 1 394 443 2 49 2.02e-11 59.3
MS.gene56798.t1 MTR_3g465910 25.424 295 180 10 59 334 63 336 6.89e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56798.t1 AT5G67150 41.850 454 242 8 2 445 3 444 1.81e-119 356
MS.gene56798.t1 AT3G50300 41.410 454 244 10 1 443 1 443 9.10e-110 332
MS.gene56798.t1 AT3G50280 39.468 451 253 8 1 443 1 439 3.08e-105 320
MS.gene56798.t1 AT5G67160 38.839 448 244 10 2 442 3 427 1.26e-101 310
MS.gene56798.t1 AT3G50270 37.969 453 257 10 5 445 6 446 7.45e-101 308
MS.gene56798.t1 AT5G38130 40.183 438 238 13 1 429 21 443 6.57e-98 301
MS.gene56798.t1 AT5G42830 37.559 426 242 9 32 443 30 445 8.21e-88 275
MS.gene56798.t1 AT5G07850 38.084 428 240 10 32 443 31 449 7.43e-86 270
MS.gene56798.t1 AT2G39980 33.691 466 245 7 30 445 27 478 1.06e-83 265
MS.gene56798.t1 AT5G07860 37.731 432 232 12 32 443 31 445 6.75e-83 263
MS.gene56798.t1 AT5G01210 34.914 464 251 8 24 448 19 470 2.39e-79 254
MS.gene56798.t1 AT3G50280 41.158 311 176 4 138 443 1 309 1.44e-76 241
MS.gene56798.t1 AT5G07870 35.991 439 242 10 32 443 31 457 2.22e-75 243
MS.gene56798.t1 AT3G50290 42.164 268 145 4 135 394 4 269 3.82e-63 206
MS.gene56798.t1 AT5G23940 32.947 431 265 8 32 444 25 449 8.25e-61 206
MS.gene56798.t1 AT3G29590 27.126 435 283 14 25 438 21 442 7.83e-28 115
MS.gene56798.t1 AT2G19070 24.612 451 288 16 24 436 7 443 1.01e-26 112
MS.gene56798.t1 AT5G48930 24.324 407 260 15 49 431 39 421 2.42e-26 110
MS.gene56798.t1 AT5G41040 26.020 392 245 14 57 430 62 426 3.18e-24 104
MS.gene56798.t1 AT5G41040 26.020 392 245 14 57 430 78 442 3.22e-24 105
MS.gene56798.t1 AT2G40230 24.888 446 288 13 1 423 1 422 1.31e-23 103
MS.gene56798.t1 AT3G29670 23.894 452 298 15 3 428 2 433 1.57e-23 102
MS.gene56798.t1 AT5G39080 24.107 448 279 16 30 436 26 453 9.42e-23 100
MS.gene56798.t1 AT3G29635 23.624 436 274 16 29 428 26 438 5.77e-22 98.6
MS.gene56798.t1 AT3G48720 26.368 402 246 18 57 436 54 427 6.90e-22 97.8
MS.gene56798.t1 AT5G39090 26.744 430 260 21 32 431 28 432 3.88e-21 95.9
MS.gene56798.t1 AT5G23970 24.829 439 274 20 28 447 20 421 1.31e-20 94.0
MS.gene56798.t1 AT5G57840 24.821 419 267 13 36 428 26 422 8.80e-20 91.7
MS.gene56798.t1 AT5G39050 25.000 328 208 13 135 431 132 452 1.16e-19 91.7
MS.gene56798.t1 AT1G24430 25.553 407 255 14 55 447 53 425 1.22e-19 91.3
MS.gene56798.t1 AT3G30280 24.516 465 301 20 4 447 1 436 6.97e-19 89.0
MS.gene56798.t1 AT5G07080 28.065 310 177 13 42 327 44 331 3.86e-18 87.0
MS.gene56798.t1 AT4G15390 22.912 467 312 18 1 447 1 439 6.13e-18 86.3
MS.gene56798.t1 AT4G15390 22.912 467 312 18 1 447 1 439 6.13e-18 86.3
MS.gene56798.t1 AT5G63560 24.365 394 241 17 57 428 54 412 9.79e-18 85.5
MS.gene56798.t1 AT1G03940 25.424 413 246 19 58 431 60 449 2.18e-17 84.7
MS.gene56798.t1 AT3G26040 24.088 411 253 16 60 447 55 429 3.55e-17 84.0
MS.gene56798.t1 AT3G03480 23.342 407 261 15 33 416 33 411 6.58e-17 83.2
MS.gene56798.t1 AT5G17540 23.632 402 250 16 33 405 23 396 1.61e-16 82.0
MS.gene56798.t1 AT1G28680 24.355 349 230 13 64 399 62 389 2.05e-16 81.6
MS.gene56798.t1 AT1G03390 25.070 359 223 15 61 397 73 407 4.56e-16 80.5
MS.gene56798.t1 AT3G47170 24.458 323 207 11 57 357 63 370 1.27e-15 79.3
MS.gene56798.t1 AT1G31490 24.648 426 237 18 61 448 59 438 6.67e-15 77.0
MS.gene56798.t1 AT1G65450 24.375 320 202 13 55 355 63 361 1.06e-14 76.3
MS.gene56798.t1 AT1G24420 24.742 388 243 16 75 447 76 429 2.01e-14 75.5
MS.gene56798.t1 AT1G24420 24.742 388 243 16 75 447 76 429 2.01e-14 75.5
MS.gene56798.t1 AT5G61160 24.194 310 202 14 148 434 139 438 3.58e-14 74.7
MS.gene56798.t1 AT1G28680 23.746 299 203 9 64 352 62 345 3.70e-14 74.3
MS.gene56798.t1 AT5G47950 24.464 466 286 22 1 447 1 419 3.89e-14 74.3
MS.gene56798.t1 AT5G47980 23.601 411 254 17 63 447 60 436 2.10e-13 72.4
MS.gene56798.t1 AT2G25150 22.590 363 239 11 59 400 62 403 5.72e-13 70.9
MS.gene56798.t1 AT4G15400 24.201 438 241 19 49 446 41 427 4.23e-12 68.2
MS.gene56798.t1 AT1G03495 27.458 295 173 14 58 323 60 342 1.16e-11 66.2
MS.gene56798.t1 AT1G32910 21.763 363 227 10 64 397 65 399 1.72e-11 66.2
MS.gene56798.t1 AT2G23510 22.767 347 242 12 64 399 69 400 2.04e-11 66.2
MS.gene56798.t1 AT5G16410 21.366 454 290 16 7 422 36 460 2.12e-11 66.2

Find 86 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGCTTGACGAGGATTATTT+TGG 0.141275 8.2:+25463926 MS.gene56798:CDS
AAGGAACATAGTTTGGTTTA+AGG 0.151696 8.2:-25463379 None:intergenic
TCTCCGAGGTTTGATGTTTA+TGG 0.199950 8.2:+25464154 MS.gene56798:CDS
GTAAAATGGAAAATTCTTTC+AGG 0.213962 8.2:-25463710 None:intergenic
CACATTCTGATGAGAAAATA+AGG 0.214956 8.2:+25464047 MS.gene56798:CDS
CGTTGAAATGAAGGGAGTTT+TGG 0.257934 8.2:-25463602 None:intergenic
GATTTGAACATTCTCTGTTT+TGG 0.269213 8.2:-25463777 None:intergenic
TGGTTTCAACTAGGAGAAAT+TGG 0.290333 8.2:-25463124 None:intergenic
GGGAAATGATGCTGAGTTCT+TGG 0.295386 8.2:+25464321 MS.gene56798:CDS
TCAAATGGTTGATTTGGTTT+TGG 0.303035 8.2:-25463184 None:intergenic
GATGTTTATGGTAATGATTT+TGG 0.307637 8.2:+25464166 MS.gene56798:CDS
GCCTGTTAAGGGTGGAAAAT+AGG 0.317928 8.2:-25463231 None:intergenic
ACGTAATGAAAATCTTCTTC+TGG 0.318483 8.2:-25463869 None:intergenic
GTTGTTTGGGATTAATAGTT+TGG 0.327840 8.2:+25464102 MS.gene56798:CDS
ACTATAGAAGGAACATAGTT+TGG 0.352065 8.2:-25463386 None:intergenic
TTTGTTTCAAATGGTTGATT+TGG 0.358387 8.2:-25463190 None:intergenic
TGATGTGTTGTTGTTGTTTG+TGG 0.358976 8.2:-25463282 None:intergenic
GATTATAAGACGGCCTGTTA+AGG 0.359476 8.2:-25463243 None:intergenic
TTTGAGATATTGGAGGCTTT+GGG 0.368507 8.2:+25464301 MS.gene56798:CDS
GGGAAGATTACTGTGTTTGC+AGG 0.373043 8.2:+25464238 MS.gene56798:CDS
ATTGGAAGTTCAACGTCGTT+TGG 0.373841 8.2:-25463632 None:intergenic
TTTGGTTTGGAATGGGTTGA+TGG 0.384443 8.2:-25463166 None:intergenic
TTTGTGATGGCAAGATGTTT+TGG 0.386713 8.2:+25463531 MS.gene56798:CDS
TTGAGAATGCCGAAGTTGTT+TGG 0.387603 8.2:+25464088 MS.gene56798:CDS
TTAGCTAATGGCATCTTCAT+TGG 0.396826 8.2:+25463488 MS.gene56798:CDS
TTTGGAAATGCAGGGATCAT+AGG 0.402401 8.2:+25463944 MS.gene56798:CDS
CATTAGCTAACTCCGTTACT+TGG 0.402418 8.2:-25463475 None:intergenic
TGGAAAGTGTTTGTTTCAAA+TGG 0.404356 8.2:-25463199 None:intergenic
TTGGAAGTTCAACGTCGTTT+GGG 0.408058 8.2:-25463631 None:intergenic
TGAGAATGCCGAAGTTGTTT+GGG 0.411706 8.2:+25464089 MS.gene56798:CDS
TTTGGCAGGTGCTAATTCAT+TGG 0.414867 8.2:+25464120 MS.gene56798:CDS
CTAGTTGAAACCATTCAAAA+AGG 0.420830 8.2:+25463134 MS.gene56798:CDS
GTAGTGTAACTATGAAAGTA+GGG 0.427730 8.2:+25463966 MS.gene56798:CDS
TTTGGCATTTCGTCAATACT+TGG 0.430278 8.2:+25463549 MS.gene56798:CDS
TTTGGGATTAATAGTTTGGC+AGG 0.440696 8.2:+25464106 MS.gene56798:CDS
TGGGATTGGAAAATGTTCAT+TGG 0.444512 8.2:+25464003 MS.gene56798:CDS
AAGCGTTGTTCTCTCATGTA+TGG 0.446035 8.2:+25463816 MS.gene56798:CDS
TTTGTGAAAGGAAAGTGTAT+TGG 0.453790 8.2:-25463650 None:intergenic
GGTAGTGTAACTATGAAAGT+AGG 0.456839 8.2:+25463965 MS.gene56798:CDS
TTGAGATTATTGTTGTCAAT+TGG 0.459438 8.2:-25463056 None:intergenic
GGAGTGCAAATAAAAGTAAT+GGG 0.465217 8.2:+25464218 MS.gene56798:CDS
CTCCCACTTCGAACCCCTAC+CGG 0.466626 8.2:-25464199 None:intergenic
ATTATAAGACGGCCTGTTAA+GGG 0.469230 8.2:-25463242 None:intergenic
GGGAACCAACGTTGAAATGA+AGG 0.474068 8.2:-25463611 None:intergenic
GGGAGTGCAAATAAAAGTAA+TGG 0.474744 8.2:+25464217 MS.gene56798:CDS
GTTTGTGGCTACGTTGTGTT+GGG 0.476915 8.2:-25463267 None:intergenic
AAACCGGTAGGGGTTCGAAG+TGG 0.479206 8.2:+25464196 MS.gene56798:CDS
CAAGTAACGGAGTTAGCTAA+TGG 0.482336 8.2:+25463476 MS.gene56798:CDS
ACCAAGGTTGTTGCAAATGA+TGG 0.487314 8.2:-25463309 None:intergenic
GTTACTTGGACTGCTAGAAT+AGG 0.498322 8.2:-25463461 None:intergenic
GAATGGGTTGATGGAAAAGA+AGG 0.508278 8.2:-25463157 None:intergenic
TGTTTGTGGCTACGTTGTGT+TGG 0.512319 8.2:-25463268 None:intergenic
CTCAACCACGTTGTTTGTGA+TGG 0.514978 8.2:+25463518 MS.gene56798:CDS
GAGATTCTAGAATGTGGGAT+TGG 0.518222 8.2:+25463989 MS.gene56798:CDS
TAGGGGAGATTCTAGAATGT+GGG 0.520801 8.2:+25463984 MS.gene56798:CDS
TCCATCATTTGCAACAACCT+TGG 0.522468 8.2:+25463308 MS.gene56798:CDS
TATTGTTGTCAATTGGTGCT+TGG 0.527153 8.2:-25463049 None:intergenic
GTAGGGGAGATTCTAGAATG+TGG 0.529927 8.2:+25463983 MS.gene56798:CDS
ACATTCTGATGAGAAAATAA+GGG 0.533534 8.2:+25464048 MS.gene56798:CDS
AACCGGTAGGGGTTCGAAGT+GGG 0.542449 8.2:+25464197 MS.gene56798:CDS
TCAAGCGGAGGAATCATCCT+TGG 0.542679 8.2:-25463911 None:intergenic
AACTCCCTTCATTTCAACGT+TGG 0.545872 8.2:+25463606 MS.gene56798:CDS
CTTTCAGGTAAGTTCTGCGT+CGG 0.565322 8.2:-25463695 None:intergenic
AATAATCGATTTAACACCAT+GGG 0.569717 8.2:+25463097 MS.gene56798:CDS
GGAACCAACGTTGAAATGAA+GGG 0.576282 8.2:-25463610 None:intergenic
GTGTTTGCAGGAGCTGAAGA+AGG 0.577173 8.2:+25464250 MS.gene56798:CDS
CTATTAATCCCAAACAACTT+CGG 0.578183 8.2:-25464097 None:intergenic
TCTCTTCGTCCACGCAAAAG+CGG 0.578199 8.2:+25463334 MS.gene56798:CDS
CCAAAATAATCCTCGTCAAG+CGG 0.581464 8.2:-25463926 None:intergenic
TAAGGGATGATTATGAGTGT+TGG 0.592031 8.2:+25464065 MS.gene56798:CDS
TAGTTATAGGTGTTAGACCA+AGG 0.600032 8.2:+25463894 MS.gene56798:CDS
ACTAGTTGAGATCACTGTCA+TGG 0.605060 8.2:-25463021 None:intergenic
AGGAGAAATTGGAGATCCCA+TGG 0.609549 8.2:-25463113 None:intergenic
AAATAATCGATTTAACACCA+TGG 0.626064 8.2:+25463096 MS.gene56798:CDS
TATTCTAGCAGTCCAAGTAA+CGG 0.629197 8.2:+25463463 MS.gene56798:CDS
TGTGTTGGGTGATTATAAGA+CGG 0.629490 8.2:-25463253 None:intergenic
TAGTGTAACTATGAAAGTAG+GGG 0.630112 8.2:+25463967 MS.gene56798:CDS
GATGATTCCTCCGCTTGACG+AGG 0.630798 8.2:+25463916 MS.gene56798:CDS
AAATAATCCTCGTCAAGCGG+AGG 0.636490 8.2:-25463923 None:intergenic
TGCTAGAATAGGTTGTGAAG+TGG 0.647856 8.2:-25463450 None:intergenic
ATAAGACGGCCTGTTAAGGG+TGG 0.648919 8.2:-25463239 None:intergenic
TGGAAAATAGGAGAGTGTTG+TGG 0.662672 8.2:-25463219 None:intergenic
TTACCATAAACATCAAACCT+CGG 0.674952 8.2:-25464157 None:intergenic
TGGCTACGAGTAGTTCTCCG+AGG 0.676435 8.2:+25464140 MS.gene56798:CDS
CGTGGACGAAGAGAGCACCA+AGG 0.726493 8.2:-25463325 None:intergenic
TCTTGCCATCACAAACAACG+TGG 0.787958 8.2:-25463523 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAATTTTCCATTTTACAA+AGG + chr8.2:25463715-25463734 MS.gene56798:CDS 20.0%
!!! TTTCATTACGTTTTAGTTAT+AGG + chr8.2:25463881-25463900 MS.gene56798:CDS 20.0%
! AAAAATGAGTGAACTATAGA+AGG - chr8.2:25463401-25463420 None:intergenic 25.0%
! ACATTCTGATGAGAAAATAA+GGG + chr8.2:25464048-25464067 MS.gene56798:CDS 25.0%
! GTAAAATGGAAAATTCTTTC+AGG - chr8.2:25463713-25463732 None:intergenic 25.0%
! TTGAGATTATTGTTGTCAAT+TGG - chr8.2:25463059-25463078 None:intergenic 25.0%
! TTTGTTTCAAATGGTTGATT+TGG - chr8.2:25463193-25463212 None:intergenic 25.0%
!! AAATAATCGATTTAACACCA+TGG + chr8.2:25463096-25463115 MS.gene56798:CDS 25.0%
!! AATAATCGATTTAACACCAT+GGG + chr8.2:25463097-25463116 MS.gene56798:CDS 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr8.2:25464166-25464185 MS.gene56798:CDS 25.0%
!!! TTATGGTAATGATTTTGGTT+GGG + chr8.2:25464171-25464190 MS.gene56798:CDS 25.0%
!!! TTGTCTTTCTTTTGAGATAT+TGG + chr8.2:25464291-25464310 MS.gene56798:CDS 25.0%
!!! TTTATGGTAATGATTTTGGT+TGG + chr8.2:25464170-25464189 MS.gene56798:CDS 25.0%
ACGTAATGAAAATCTTCTTC+TGG - chr8.2:25463872-25463891 None:intergenic 30.0%
ACTATAGAAGGAACATAGTT+TGG - chr8.2:25463389-25463408 None:intergenic 30.0%
CACATTCTGATGAGAAAATA+AGG + chr8.2:25464047-25464066 MS.gene56798:CDS 30.0%
CTAGTTGAAACCATTCAAAA+AGG + chr8.2:25463134-25463153 MS.gene56798:CDS 30.0%
CTATTAATCCCAAACAACTT+CGG - chr8.2:25464100-25464119 None:intergenic 30.0%
GGAGTGCAAATAAAAGTAAT+GGG + chr8.2:25464218-25464237 MS.gene56798:CDS 30.0%
GTAGTGTAACTATGAAAGTA+GGG + chr8.2:25463966-25463985 MS.gene56798:CDS 30.0%
TAGTGTAACTATGAAAGTAG+GGG + chr8.2:25463967-25463986 MS.gene56798:CDS 30.0%
TTACCATAAACATCAAACCT+CGG - chr8.2:25464160-25464179 None:intergenic 30.0%
! AAGGAACATAGTTTGGTTTA+AGG - chr8.2:25463382-25463401 None:intergenic 30.0%
! CGATTTTCTCCTTTGTAAAA+TGG - chr8.2:25463727-25463746 None:intergenic 30.0%
! CTTTTTGAATGGTTTCAACT+AGG - chr8.2:25463136-25463155 None:intergenic 30.0%
! GATTTGAACATTCTCTGTTT+TGG - chr8.2:25463780-25463799 None:intergenic 30.0%
! GTTGTTTGGGATTAATAGTT+TGG + chr8.2:25464102-25464121 MS.gene56798:CDS 30.0%
! TTTGTGAAAGGAAAGTGTAT+TGG - chr8.2:25463653-25463672 None:intergenic 30.0%
! TTTTACAAAGGAGAAAATCG+CGG + chr8.2:25463727-25463746 MS.gene56798:CDS 30.0%
!! AGTTTTTAACTCCGTTAAGT+GGG - chr8.2:25463424-25463443 None:intergenic 30.0%
!! TAGTTTTTAACTCCGTTAAG+TGG - chr8.2:25463425-25463444 None:intergenic 30.0%
!! TGGAAAGTGTTTGTTTCAAA+TGG - chr8.2:25463202-25463221 None:intergenic 30.0%
!!! AGGATTATTTTGGAAATGCA+GGG + chr8.2:25463936-25463955 MS.gene56798:CDS 30.0%
!!! ATTTGGTTTTGGTTTGGAAT+GGG - chr8.2:25463176-25463195 None:intergenic 30.0%
!!! TATGGTAATGATTTTGGTTG+GGG + chr8.2:25464172-25464191 MS.gene56798:CDS 30.0%
!!! TCAAATGGTTGATTTGGTTT+TGG - chr8.2:25463187-25463206 None:intergenic 30.0%
!!! TCTTTCTTTTGAGATATTGG+AGG + chr8.2:25464294-25464313 MS.gene56798:CDS 30.0%
ATTATAAGACGGCCTGTTAA+GGG - chr8.2:25463245-25463264 None:intergenic 35.0%
GGTAGTGTAACTATGAAAGT+AGG + chr8.2:25463965-25463984 MS.gene56798:CDS 35.0%
TAAGGGATGATTATGAGTGT+TGG + chr8.2:25464065-25464084 MS.gene56798:CDS 35.0%
TAGTTATAGGTGTTAGACCA+AGG + chr8.2:25463894-25463913 MS.gene56798:CDS 35.0%
TATTCTAGCAGTCCAAGTAA+CGG + chr8.2:25463463-25463482 MS.gene56798:CDS 35.0%
TGGGATTGGAAAATGTTCAT+TGG + chr8.2:25464003-25464022 MS.gene56798:CDS 35.0%
TGGTTTCAACTAGGAGAAAT+TGG - chr8.2:25463127-25463146 None:intergenic 35.0%
TGTGTTGGGTGATTATAAGA+CGG - chr8.2:25463256-25463275 None:intergenic 35.0%
TTAGCTAATGGCATCTTCAT+TGG + chr8.2:25463488-25463507 MS.gene56798:CDS 35.0%
TTTGGCATTTCGTCAATACT+TGG + chr8.2:25463549-25463568 MS.gene56798:CDS 35.0%
! CGGTTTTCTACATTGTGATT+CGG - chr8.2:25463678-25463697 None:intergenic 35.0%
! GAAAAGAAGGCCTTTTTGAA+TGG - chr8.2:25463147-25463166 None:intergenic 35.0%
! GGGAGTGCAAATAAAAGTAA+TGG + chr8.2:25464217-25464236 MS.gene56798:CDS 35.0%
! TATTGTTGTCAATTGGTGCT+TGG - chr8.2:25463052-25463071 None:intergenic 35.0%
! TTTGAGATATTGGAGGCTTT+GGG + chr8.2:25464301-25464320 MS.gene56798:CDS 35.0%
! TTTGGGATTAATAGTTTGGC+AGG + chr8.2:25464106-25464125 MS.gene56798:CDS 35.0%
! TTTGTGATGGCAAGATGTTT+TGG + chr8.2:25463531-25463550 MS.gene56798:CDS 35.0%
!! CACTCATTTTTCCCACTTAA+CGG + chr8.2:25463410-25463429 MS.gene56798:CDS 35.0%
!! TGATGTGTTGTTGTTGTTTG+TGG - chr8.2:25463285-25463304 None:intergenic 35.0%
!!! ATGGTAATGATTTTGGTTGG+GGG + chr8.2:25464173-25464192 MS.gene56798:CDS 35.0%
!!! GAGGATTATTTTGGAAATGC+AGG + chr8.2:25463935-25463954 MS.gene56798:CDS 35.0%
!!! GATTTGGTTTTGGTTTGGAA+TGG - chr8.2:25463177-25463196 None:intergenic 35.0%
!!! TGGTTGATTTGGTTTTGGTT+TGG - chr8.2:25463182-25463201 None:intergenic 35.0%
!!! TGTGATTCGGTTTTTGTGAA+AGG - chr8.2:25463665-25463684 None:intergenic 35.0%
!!! TTTTGAGATATTGGAGGCTT+TGG + chr8.2:25464300-25464319 MS.gene56798:CDS 35.0%
AACTCCCTTCATTTCAACGT+TGG + chr8.2:25463606-25463625 MS.gene56798:CDS 40.0%
ACCAAGGTTGTTGCAAATGA+TGG - chr8.2:25463312-25463331 None:intergenic 40.0%
ACTAGTTGAGATCACTGTCA+TGG - chr8.2:25463024-25463043 None:intergenic 40.0%
ATTGGAAGTTCAACGTCGTT+TGG - chr8.2:25463635-25463654 None:intergenic 40.0%
CATTAGCTAACTCCGTTACT+TGG - chr8.2:25463478-25463497 None:intergenic 40.0%
CCAAAATAATCCTCGTCAAG+CGG - chr8.2:25463929-25463948 None:intergenic 40.0%
GATTATAAGACGGCCTGTTA+AGG - chr8.2:25463246-25463265 None:intergenic 40.0%
GGAACCAACGTTGAAATGAA+GGG - chr8.2:25463613-25463632 None:intergenic 40.0%
GTTACTTGGACTGCTAGAAT+AGG - chr8.2:25463464-25463483 None:intergenic 40.0%
TCCATCATTTGCAACAACCT+TGG + chr8.2:25463308-25463327 MS.gene56798:CDS 40.0%
TCTCCGAGGTTTGATGTTTA+TGG + chr8.2:25464154-25464173 MS.gene56798:CDS 40.0%
TGAGAATGCCGAAGTTGTTT+GGG + chr8.2:25464089-25464108 MS.gene56798:CDS 40.0%
TGCTAGAATAGGTTGTGAAG+TGG - chr8.2:25463453-25463472 None:intergenic 40.0%
TTGAGAATGCCGAAGTTGTT+TGG + chr8.2:25464088-25464107 MS.gene56798:CDS 40.0%
TTGGAAGTTCAACGTCGTTT+GGG - chr8.2:25463634-25463653 None:intergenic 40.0%
TTTGGAAATGCAGGGATCAT+AGG + chr8.2:25463944-25463963 MS.gene56798:CDS 40.0%
TTTGGCAGGTGCTAATTCAT+TGG + chr8.2:25464120-25464139 MS.gene56798:CDS 40.0%
! AAGCGTTGTTCTCTCATGTA+TGG + chr8.2:25463816-25463835 MS.gene56798:CDS 40.0%
! CAAGTAACGGAGTTAGCTAA+TGG + chr8.2:25463476-25463495 MS.gene56798:CDS 40.0%
! CGTTGAAATGAAGGGAGTTT+TGG - chr8.2:25463605-25463624 None:intergenic 40.0%
! TCCTATTTTCCACCCTTAAC+AGG + chr8.2:25463230-25463249 MS.gene56798:CDS 40.0%
!! AAGGGAGTTTTGGTATACTC+TGG - chr8.2:25463595-25463614 None:intergenic 40.0%
!! AGGGAGTTTTGGTATACTCT+GGG - chr8.2:25463594-25463613 None:intergenic 40.0%
!! GAATGGGTTGATGGAAAAGA+AGG - chr8.2:25463160-25463179 None:intergenic 40.0%
!! GAGATTCTAGAATGTGGGAT+TGG + chr8.2:25463989-25464008 MS.gene56798:CDS 40.0%
!! TAGGGGAGATTCTAGAATGT+GGG + chr8.2:25463984-25464003 MS.gene56798:CDS 40.0%
!! TGGAAAATAGGAGAGTGTTG+TGG - chr8.2:25463222-25463241 None:intergenic 40.0%
!! TTTGGTTTGGAATGGGTTGA+TGG - chr8.2:25463169-25463188 None:intergenic 40.0%
AAATAATCCTCGTCAAGCGG+AGG - chr8.2:25463926-25463945 None:intergenic 45.0%
AGGAGAAATTGGAGATCCCA+TGG - chr8.2:25463116-25463135 None:intergenic 45.0%
CTCAACCACGTTGTTTGTGA+TGG + chr8.2:25463518-25463537 MS.gene56798:CDS 45.0%
GCCTGTTAAGGGTGGAAAAT+AGG - chr8.2:25463234-25463253 None:intergenic 45.0%
GGGAAATGATGCTGAGTTCT+TGG + chr8.2:25464321-25464340 MS.gene56798:CDS 45.0%
GGGAACCAACGTTGAAATGA+AGG - chr8.2:25463614-25463633 None:intergenic 45.0%
GGGAAGATTACTGTGTTTGC+AGG + chr8.2:25464238-25464257 MS.gene56798:CDS 45.0%
GTTTGTGGCTACGTTGTGTT+GGG - chr8.2:25463270-25463289 None:intergenic 45.0%
TCTTGCCATCACAAACAACG+TGG - chr8.2:25463526-25463545 None:intergenic 45.0%
TGTTTGTGGCTACGTTGTGT+TGG - chr8.2:25463271-25463290 None:intergenic 45.0%
! CCGCTTGACGAGGATTATTT+TGG + chr8.2:25463926-25463945 MS.gene56798:CDS 45.0%
! CTTTCAGGTAAGTTCTGCGT+CGG - chr8.2:25463698-25463717 None:intergenic 45.0%
!! GTAGGGGAGATTCTAGAATG+TGG + chr8.2:25463983-25464002 MS.gene56798:CDS 45.0%
!! TTGTGTTTTCCGCTTTTGCG+TGG - chr8.2:25463346-25463365 None:intergenic 45.0%
!!! TGATTTTGGTTGGGGGAAAC+CGG + chr8.2:25464180-25464199 MS.gene56798:CDS 45.0%
ATAAGACGGCCTGTTAAGGG+TGG - chr8.2:25463242-25463261 None:intergenic 50.0%
GTGTTTGCAGGAGCTGAAGA+AGG + chr8.2:25464250-25464269 MS.gene56798:CDS 50.0%
TCAAGCGGAGGAATCATCCT+TGG - chr8.2:25463914-25463933 None:intergenic 50.0%
TCTCTTCGTCCACGCAAAAG+CGG + chr8.2:25463334-25463353 MS.gene56798:CDS 50.0%
AAACCGGTAGGGGTTCGAAG+TGG + chr8.2:25464196-25464215 MS.gene56798:CDS 55.0%
AACCGGTAGGGGTTCGAAGT+GGG + chr8.2:25464197-25464216 MS.gene56798:CDS 55.0%
GATGATTCCTCCGCTTGACG+AGG + chr8.2:25463916-25463935 MS.gene56798:CDS 55.0%
TGGCTACGAGTAGTTCTCCG+AGG + chr8.2:25464140-25464159 MS.gene56798:CDS 55.0%
TTGGTTGGGGGAAACCGGTA+GGG + chr8.2:25464185-25464204 MS.gene56798:CDS 55.0%
TTTGGTTGGGGGAAACCGGT+AGG + chr8.2:25464184-25464203 MS.gene56798:CDS 55.0%
CTCCCACTTCGAACCCCTAC+CGG - chr8.2:25464202-25464221 None:intergenic 60.0%
TGGTTGGGGGAAACCGGTAG+GGG + chr8.2:25464186-25464205 MS.gene56798:CDS 60.0%
!! CGTGGACGAAGAGAGCACCA+AGG - chr8.2:25463328-25463347 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 25463014 25464360 25463014 ID=MS.gene56798
chr8.2 mRNA 25463014 25464360 25463014 ID=MS.gene56798.t1;Parent=MS.gene56798
chr8.2 exon 25463014 25464360 25463014 ID=MS.gene56798.t1.exon1;Parent=MS.gene56798.t1
chr8.2 CDS 25463014 25464360 25463014 ID=cds.MS.gene56798.t1;Parent=MS.gene56798.t1
Gene Sequence

>MS.gene56798

ATGGATTCCATGACAGTGATCTCAACTAGTACAATCCAAGCACCAATTGACAACAATAATCTCAACTCAAGAAGTGATTATCAAATAATCGATTTAACACCATGGGATCTCCAATTTCTCCTAGTTGAAACCATTCAAAAAGGCCTTCTTTTCCATCAACCCATTCCAAACCAAAACCAAATCAACCATTTGAAACAAACACTTTCCACAACACTCTCCTATTTTCCACCCTTAACAGGCCGTCTTATAATCACCCAACACAACGTAGCCACAAACAACAACAACACATCATGTTCCATCATTTGCAACAACCTTGGTGCTCTCTTCGTCCACGCAAAAGCGGAAAACACAACTGTATCAGACATCCTTAAACCAAACTATGTTCCTTCTATAGTTCACTCATTTTTCCCACTTAACGGAGTTAAAAACTACGAAGCCACTTCACAACCTATTCTAGCAGTCCAAGTAACGGAGTTAGCTAATGGCATCTTCATTGGTTTGACTCTCAACCACGTTGTTTGTGATGGCAAGATGTTTTGGCATTTCGTCAATACTTGGTCACAAATCTCAAAAAACTCCCAGAGTATACCAAAACTCCCTTCATTTCAACGTTGGTTCCCAAACGACGTTGAACTTCCAATACACTTTCCTTTCACAAAAACCGAATCACAATGTAGAAAACCGACGCAGAACTTACCTGAAAGAATTTTCCATTTTACAAAGGAGAAAATCGCGGAACTGAAATCAAAAGCAAATGAAGAAACCAAAACAGAGAATGTTCAAATCAAAATATCTTCGCTTCAAGCGTTGTTCTCTCATGTATGGCGATGTGTTGTTCGAAGCAAAAAACTCGATCCAGAAGAAGATTTTCATTACGTTTTAGTTATAGGTGTTAGACCAAGGATGATTCCTCCGCTTGACGAGGATTATTTTGGAAATGCAGGGATCATAGGTAGTGTAACTATGAAAGTAGGGGAGATTCTAGAATGTGGGATTGGAAAATGTTCATTGGAGATGAACAAGATGATTATGTCACATTCTGATGAGAAAATAAGGGATGATTATGAGTGTTGGTTGAGAATGCCGAAGTTGTTTGGGATTAATAGTTTGGCAGGTGCTAATTCATTGGCTACGAGTAGTTCTCCGAGGTTTGATGTTTATGGTAATGATTTTGGTTGGGGGAAACCGGTAGGGGTTCGAAGTGGGAGTGCAAATAAAAGTAATGGGAAGATTACTGTGTTTGCAGGAGCTGAAGAAGGTAGCATTGATATTGAAGTTTGTCTTTCTTTTGAGATATTGGAGGCTTTGGGAAATGATGCTGAGTTCTTGGTTCATAGTTCTAAGTAA
Protein sequence

>MS.gene56798.t1

MDSMTVISTSTIQAPIDNNNLNSRSDYQIIDLTPWDLQFLLVETIQKGLLFHQPIPNQNQINHLKQTLSTTLSYFPPLTGRLIITQHNVATNNNNTSCSIICNNLGALFVHAKAENTTVSDILKPNYVPSIVHSFFPLNGVKNYEATSQPILAVQVTELANGIFIGLTLNHVVCDGKMFWHFVNTWSQISKNSQSIPKLPSFQRWFPNDVELPIHFPFTKTESQCRKPTQNLPERIFHFTKEKIAELKSKANEETKTENVQIKISSLQALFSHVWRCVVRSKKLDPEEDFHYVLVIGVRPRMIPPLDEDYFGNAGIIGSVTMKVGEILECGIGKCSLEMNKMIMSHSDEKIRDDYECWLRMPKLFGINSLAGANSLATSSSPRFDVYGNDFGWGKPVGVRSGSANKSNGKITVFAGAEEGSIDIEVCLSFEILEALGNDAEFLVHSSK