AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99219

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99219.t1 MTR_4g094828 90.949 453 39 2 1 453 1 451 0.0 854
MS.gene99219.t1 MTR_4g022290 83.407 452 64 3 1 450 1 443 0.0 768
MS.gene99219.t1 MTR_8g079710 74.115 452 102 7 1 448 1 441 0.0 665
MS.gene99219.t1 MTR_4g094835 54.746 453 181 12 1 444 1 438 4.61e-167 478
MS.gene99219.t1 MTR_4g094842 53.070 456 186 8 1 444 1 440 2.05e-159 458
MS.gene99219.t1 MTR_0190s0020 49.561 456 212 5 1 452 1 442 2.61e-151 437
MS.gene99219.t1 MTR_3g088420 50.877 456 197 11 1 452 1 433 4.74e-151 436
MS.gene99219.t1 MTR_4g022800 50.756 463 196 13 1 452 1 442 6.24e-149 431
MS.gene99219.t1 MTR_4g022825 50.873 458 199 12 1 451 1 439 4.46e-148 429
MS.gene99219.t1 MTR_3g026310 50.107 467 199 11 1 452 1 448 1.43e-147 428
MS.gene99219.t1 MTR_4g022720 50.549 455 198 13 1 450 1 433 3.84e-147 427
MS.gene99219.t1 MTR_8g041770 49.563 458 208 9 1 452 1 441 2.33e-146 425
MS.gene99219.t1 MTR_4g094838 50.442 452 181 11 6 444 3 424 2.54e-146 424
MS.gene99219.t1 MTR_4g022670 50.217 460 202 12 1 452 1 441 6.76e-144 419
MS.gene99219.t1 MTR_7g026860 50.109 459 197 13 1 450 1 436 9.14e-144 418
MS.gene99219.t1 MTR_4g094832 54.712 382 147 9 73 444 57 422 1.50e-137 402
MS.gene99219.t1 MTR_4g022760 43.791 459 189 10 1 452 1 397 1.09e-117 350
MS.gene99219.t1 MTR_6g016815 41.630 454 176 10 1 452 15 381 2.98e-104 315
MS.gene99219.t1 MTR_3g101900 37.443 438 243 12 29 453 30 449 3.53e-81 258
MS.gene99219.t1 MTR_3g101930 37.415 441 236 13 25 444 26 447 4.30e-81 258
MS.gene99219.t1 MTR_3g102020 36.266 466 258 15 6 453 8 452 1.11e-77 249
MS.gene99219.t1 MTR_3g102010 36.180 445 250 13 25 453 26 452 2.24e-77 248
MS.gene99219.t1 MTR_3g101960 35.763 439 246 12 23 444 23 442 2.13e-75 243
MS.gene99219.t1 MTR_3g101970 35.045 448 242 13 23 453 23 438 4.26e-75 242
MS.gene99219.t1 MTR_8g040760 52.995 217 94 4 1 212 1 214 6.03e-69 219
MS.gene99219.t1 MTR_5g081960 33.128 486 263 15 6 444 8 478 7.55e-66 219
MS.gene99219.t1 MTR_3g070170 32.051 468 268 12 13 448 17 466 6.17e-62 208
MS.gene99219.t1 MTR_4g094825 55.556 189 76 5 20 202 83 269 9.66e-62 206
MS.gene99219.t1 MTR_4g094825 50.000 146 59 4 300 444 251 383 5.03e-36 137
MS.gene99219.t1 MTR_4g015180 31.474 502 269 15 8 452 4 487 1.83e-59 202
MS.gene99219.t1 MTR_7g026850 59.868 152 59 2 34 184 12 162 3.75e-57 187
MS.gene99219.t1 MTR_8g040780 44.749 219 106 7 230 444 17 224 4.52e-50 171
MS.gene99219.t1 MTR_6g034495 66.379 116 37 1 70 183 45 160 2.08e-46 158
MS.gene99219.t1 MTR_3g102030 32.759 348 178 13 70 405 17 320 6.89e-44 156
MS.gene99219.t1 MTR_6g045553 29.018 448 272 12 25 442 31 462 6.47e-38 144
MS.gene99219.t1 MTR_7g026770 40.526 190 81 4 146 335 132 289 1.80e-34 130
MS.gene99219.t1 MTR_7g026830 67.073 82 27 0 89 170 16 97 1.37e-32 119
MS.gene99219.t1 MTR_6g034485 39.568 139 70 3 191 329 1 125 1.49e-26 103
MS.gene99219.t1 MTR_8g075610 25.373 402 246 16 59 441 47 413 2.43e-26 110
MS.gene99219.t1 MTR_2g105330 25.547 411 263 15 46 439 39 423 3.24e-25 107
MS.gene99219.t1 MTR_2g064495 25.791 411 262 16 46 439 39 423 4.27e-25 107
MS.gene99219.t1 MTR_4g007540 25.721 416 265 16 46 443 39 428 1.65e-24 105
MS.gene99219.t1 MTR_7g085930 25.362 414 263 14 49 431 49 447 2.64e-21 96.7
MS.gene99219.t1 MTR_8g096530 26.050 357 216 12 59 400 59 382 4.60e-21 95.5
MS.gene99219.t1 MTR_3g049150 24.880 418 258 16 46 433 38 429 9.89e-21 94.7
MS.gene99219.t1 MTR_1g040175 24.317 366 245 12 45 401 45 387 2.91e-20 93.2
MS.gene99219.t1 MTR_3g098980 25.000 356 224 14 59 402 66 390 6.85e-20 92.0
MS.gene99219.t1 MTR_7g027175 25.954 393 258 14 49 434 58 424 2.08e-19 90.5
MS.gene99219.t1 MTR_5g026320 26.222 450 259 20 26 444 38 445 2.14e-19 90.9
MS.gene99219.t1 MTR_7g085990 24.096 415 283 12 49 441 45 449 2.53e-19 90.5
MS.gene99219.t1 MTR_7g014200 23.736 455 269 16 27 433 28 452 8.34e-18 85.9
MS.gene99219.t1 MTR_4g097090 24.824 427 251 19 46 433 45 440 1.05e-16 82.4
MS.gene99219.t1 MTR_2g089765 22.353 425 254 16 26 400 36 434 3.06e-16 81.3
MS.gene99219.t1 MTR_7g014160 24.888 446 274 17 29 433 30 455 1.24e-15 79.3
MS.gene99219.t1 MTR_7g014330 23.833 407 260 15 29 404 26 413 1.30e-15 79.3
MS.gene99219.t1 MTR_3g028210 23.009 452 297 17 21 439 21 454 3.33e-15 77.8
MS.gene99219.t1 MTR_6g015315 23.058 399 258 15 33 403 30 407 6.24e-15 77.0
MS.gene99219.t1 MTR_1g081660 23.821 403 240 16 29 401 31 396 8.60e-15 76.6
MS.gene99219.t1 MTR_5g090880 23.797 374 228 12 59 401 67 414 1.10e-14 76.3
MS.gene99219.t1 MTR_7g014610 22.698 467 277 18 32 443 32 469 1.14e-14 76.3
MS.gene99219.t1 MTR_8g012750 23.614 415 262 16 26 400 23 422 1.16e-14 76.3
MS.gene99219.t1 MTR_6g015815 22.907 454 278 16 27 441 32 452 1.35e-14 75.9
MS.gene99219.t1 MTR_6g015320 21.647 425 264 16 23 403 10 409 1.84e-14 75.5
MS.gene99219.t1 MTR_5g090810 22.829 403 247 13 37 401 36 412 1.98e-14 75.5
MS.gene99219.t1 MTR_7g014250 23.342 407 254 15 33 404 30 413 2.09e-14 75.5
MS.gene99219.t1 MTR_3g112180 23.188 414 286 12 33 439 38 426 2.26e-14 75.5
MS.gene99219.t1 MTR_4g127650 23.704 405 249 20 59 433 66 440 2.50e-14 75.1
MS.gene99219.t1 MTR_7g026780 40.708 113 40 5 340 452 26 111 2.70e-14 69.3
MS.gene99219.t1 MTR_6g015830 24.099 444 281 16 29 441 34 452 1.05e-13 73.2
MS.gene99219.t1 MTR_5g090830 23.325 403 245 14 37 401 36 412 1.08e-13 73.2
MS.gene99219.t1 MTR_6g015850 22.049 449 285 17 27 441 32 449 1.40e-13 72.8
MS.gene99219.t1 MTR_7g114490 24.379 443 274 19 31 442 25 437 4.01e-13 71.6
MS.gene99219.t1 MTR_2g089670 25.250 400 231 20 41 400 58 429 5.15e-13 71.2
MS.gene99219.t1 MTR_7g114520 23.441 401 260 16 31 408 26 402 7.15e-13 70.9
MS.gene99219.t1 MTR_8g079980 24.543 383 248 16 52 425 52 402 1.39e-12 69.7
MS.gene99219.t1 MTR_2g089650 23.261 417 276 16 42 429 42 443 3.97e-12 68.6
MS.gene99219.t1 MTR_7g114550 28.326 233 142 9 31 247 25 248 9.60e-12 67.0
MS.gene99219.t1 MTR_6g053810 25.649 308 207 10 59 360 71 362 1.02e-11 67.0
MS.gene99219.t1 MTR_7g026880 56.364 55 21 2 398 452 2 53 7.28e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99219.t1 AT5G67150 38.312 462 254 13 4 453 5 447 1.32e-95 295
MS.gene99219.t1 AT3G50270 36.797 462 266 13 2 453 4 449 1.62e-87 275
MS.gene99219.t1 AT3G50300 38.702 447 237 14 1 433 1 424 2.02e-85 269
MS.gene99219.t1 AT5G67160 38.084 428 229 12 25 444 25 424 2.13e-85 268
MS.gene99219.t1 AT3G50280 34.590 451 272 10 1 444 1 435 1.43e-80 256
MS.gene99219.t1 AT5G38130 36.569 443 250 11 1 433 21 442 1.73e-80 256
MS.gene99219.t1 AT5G42830 34.852 439 254 12 29 453 30 450 5.59e-76 244
MS.gene99219.t1 AT5G07860 35.632 435 251 10 29 451 31 448 3.51e-71 232
MS.gene99219.t1 AT5G07850 33.636 440 259 12 29 452 31 453 7.77e-69 226
MS.gene99219.t1 AT5G07870 35.874 446 249 13 29 452 31 461 1.70e-66 220
MS.gene99219.t1 AT2G39980 31.733 479 265 12 19 452 19 480 1.50e-65 218
MS.gene99219.t1 AT5G01210 32.334 467 263 12 22 452 20 469 5.37e-65 217
MS.gene99219.t1 AT3G50280 35.783 313 183 7 130 438 1 299 3.15e-56 189
MS.gene99219.t1 AT3G50290 38.434 281 155 7 124 398 1 269 2.10e-50 172
MS.gene99219.t1 AT5G23940 31.034 435 268 11 25 441 21 441 1.93e-46 167
MS.gene99219.t1 AT5G41040 26.617 402 254 16 59 448 69 441 2.87e-25 108
MS.gene99219.t1 AT5G41040 26.667 405 250 16 59 448 85 457 3.10e-25 108
MS.gene99219.t1 AT3G48720 25.899 417 264 17 39 440 37 423 3.84e-24 104
MS.gene99219.t1 AT5G48930 26.699 412 246 18 46 433 39 418 1.18e-23 103
MS.gene99219.t1 AT5G63560 24.359 390 244 13 59 434 61 413 2.18e-22 99.4
MS.gene99219.t1 AT2G19070 26.577 444 270 21 29 441 25 443 6.53e-20 92.4
MS.gene99219.t1 AT3G29590 22.955 440 293 14 27 443 26 442 5.63e-18 86.3
MS.gene99219.t1 AT1G78990 25.203 369 233 14 48 401 56 396 6.06e-18 86.3
MS.gene99219.t1 AT1G32910 24.860 358 231 13 59 401 65 399 9.16e-18 85.9
MS.gene99219.t1 AT5G47950 25.758 396 235 14 55 438 57 405 3.50e-17 84.0
MS.gene99219.t1 AT5G39080 23.571 420 277 16 13 403 12 416 4.53e-16 80.5
MS.gene99219.t1 AT5G16410 23.940 401 245 16 24 401 56 419 3.70e-15 77.8
MS.gene99219.t1 AT3G03480 25.381 394 241 16 31 401 34 397 5.44e-15 77.4
MS.gene99219.t1 AT2G40230 24.190 401 259 15 20 403 17 389 2.67e-14 75.1
MS.gene99219.t1 AT1G24420 26.074 349 200 17 70 403 76 381 2.68e-14 75.1
MS.gene99219.t1 AT1G24420 26.074 349 200 17 70 403 76 381 2.68e-14 75.1
MS.gene99219.t1 AT5G23970 24.171 422 252 17 45 444 38 413 3.19e-14 74.7
MS.gene99219.t1 AT3G26040 26.055 403 231 15 53 433 53 410 3.22e-14 74.7
MS.gene99219.t1 AT5G47980 24.524 420 235 17 58 445 60 429 2.60e-12 68.9
MS.gene99219.t1 AT5G61160 21.481 405 266 15 26 401 22 403 3.02e-11 65.5
MS.gene99219.t1 AT1G31490 24.318 403 250 17 18 401 18 384 5.31e-11 64.7

Find 92 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAAAGCTAATGCAGAAAT+TGG 0.278916 8.4:+26258191 MS.gene99219:CDS
GGATCTCAAATATCTTAAAA+TGG 0.314276 8.4:+26257545 MS.gene99219:CDS
GTTGGATTTAAAAGAAATAT+TGG 0.317499 8.4:+26258668 MS.gene99219:CDS
GTGTAGACGCCAAATCATTT+TGG 0.321096 8.4:+26257943 MS.gene99219:CDS
ATGGTCGATTTGGTTTGTTT+TGG 0.322357 8.4:-26257601 None:intergenic
AATGCAAGTAGGGAAATTAA+TGG 0.338622 8.4:+26258424 MS.gene99219:CDS
ACACCATTTCTAACTGCAAT+AGG 0.350294 8.4:-26258647 None:intergenic
TTTGGCATTTCATCAACTCT+TGG 0.368556 8.4:+26257961 MS.gene99219:CDS
TTGGCATTTCATCAACTCTT+GGG 0.376104 8.4:+26257962 MS.gene99219:CDS
ATGGAACATGAGGCGTTGTT+AGG 0.379377 8.4:-26257702 None:intergenic
TTTGTTTAAGATGGTCGATT+TGG 0.383044 8.4:-26257611 None:intergenic
GTTGAAAACCCCGTCTATAT+CGG 0.384392 8.4:+26258538 MS.gene99219:CDS
AAGAATAATGAGGAAACAAT+AGG 0.387662 8.4:-26257813 None:intergenic
TCACCACGGTTCAATTATTA+CGG 0.389485 8.4:+26258602 MS.gene99219:CDS
TTATGATACTTTGGAGGCTA+TGG 0.393341 8.4:+26258751 MS.gene99219:CDS
ATGGGACGATTAATGTTGTT+TGG 0.400321 8.4:-26258044 None:intergenic
TCGTGTTACCCGTAGATTGT+AGG 0.401751 8.4:+26258333 MS.gene99219:CDS
TTACGGTAATGACTTTGGTT+GGG 0.403096 8.4:+26258619 MS.gene99219:CDS
TTAATCGACGGTATCTTCAT+TGG 0.408333 8.4:+26257900 MS.gene99219:CDS
GGAAGTGGAATCGGAGCAAC+TGG 0.414902 8.4:+26258392 MS.gene99219:CDS
GATGAAATGCCAAAATGATT+TGG 0.418439 8.4:-26257952 None:intergenic
AAAATGGCACCAATCCAAAA+AGG 0.433344 8.4:+26257561 MS.gene99219:CDS
TTGTCTTCCTTATGATACTT+TGG 0.434172 8.4:+26258742 MS.gene99219:CDS
AATTATTACGGTAATGACTT+TGG 0.438421 8.4:+26258614 MS.gene99219:CDS
CTTTGTGAAAGGAAACTGTA+TGG 0.444172 8.4:-26258063 None:intergenic
TGGAAAGTGTTTGTTTAAGA+TGG 0.447960 8.4:-26257620 None:intergenic
ATTACGGTAATGACTTTGGT+TGG 0.453916 8.4:+26258618 MS.gene99219:CDS
GAGGAACATACTTTGGTTGA+AGG 0.461926 8.4:-26257791 None:intergenic
CATTCTTGTCCTACAATCTA+CGG 0.463033 8.4:-26258342 None:intergenic
TCTTCCTTATGATACTTTGG+AGG 0.463918 8.4:+26258745 MS.gene99219:CDS
TACGGTAATGACTTTGGTTG+GGG 0.464169 8.4:+26258620 MS.gene99219:CDS
GGAAACCAACGTTTGAAAGA+AGG 0.465248 8.4:-26258023 None:intergenic
TCGAATCTTCCACTTTACTA+AGG 0.472625 8.4:+26258148 MS.gene99219:CDS
AAGCCTATTGCAGTTAGAAA+TGG 0.478020 8.4:+26258644 MS.gene99219:CDS
ACGAAAGCAGACCTTTCTTC+TGG 0.482415 8.4:-26258314 None:intergenic
ACAATAGGAGGAACATACTT+TGG 0.484514 8.4:-26257798 None:intergenic
GAAACCAACGTTTGAAAGAA+GGG 0.487203 8.4:-26258022 None:intergenic
AACTTCTTTCCACCACTCGC+TGG 0.495410 8.4:+26257651 MS.gene99219:CDS
GTAGGGAAATTAATGGAAAG+TGG 0.496846 8.4:+26258431 MS.gene99219:CDS
GGTAGAGTTATTGTGCTTGG+TGG 0.507052 8.4:+26258689 MS.gene99219:CDS
TATGATACTTTGGAGGCTAT+GGG 0.511273 8.4:+26258752 MS.gene99219:CDS
GAATTTGTTCCTTAGTAAAG+TGG 0.513443 8.4:-26258157 None:intergenic
GATATGTAATAATGCAAGTA+GGG 0.513879 8.4:+26258414 MS.gene99219:CDS
ATAGCCTCCAAAGTATCATA+AGG 0.518040 8.4:-26258749 None:intergenic
TTAAGATATTTGAGATCCCA+TGG 0.520479 8.4:-26257540 None:intergenic
AAATTAATGGAAAGTGGGAT+TGG 0.520728 8.4:+26258437 MS.gene99219:CDS
TACCTGGTTCCGATATAGAC+GGG 0.523820 8.4:-26258547 None:intergenic
TACTTATAATTAAACGCCAA+AGG 0.529613 8.4:-26258277 None:intergenic
TAGTGAGTAATTATGAGTCT+TGG 0.531485 8.4:+26258516 MS.gene99219:CDS
ATTGGTAGAGTTATTGTGCT+TGG 0.531528 8.4:+26258686 MS.gene99219:CDS
GGATATGTAATAATGCAAGT+AGG 0.531741 8.4:+26258413 MS.gene99219:CDS
TAGGGAAATTAATGGAAAGT+GGG 0.533012 8.4:+26258432 MS.gene99219:CDS
AAAATCTTACACCAGAAGAA+AGG 0.535334 8.4:+26258303 MS.gene99219:CDS
ATTGCAGTTAGAAATGGTGT+TGG 0.538305 8.4:+26258650 MS.gene99219:CDS
GATTCACTCAACACCATGGC+AGG 0.542716 8.4:+26257435 None:intergenic
AAATCCCTTCTTTCAAACGT+TGG 0.543103 8.4:+26258018 MS.gene99219:CDS
AATTATCGATTTAGCACCAT+GGG 0.552815 8.4:+26257524 MS.gene99219:CDS
ACCAGCGTTGTTGCAAATGA+TGG 0.553843 8.4:-26257721 None:intergenic
GTTACTTGTACTGCAAGTAA+TGG 0.555096 8.4:-26257873 None:intergenic
CATACAGTTTCCTTTCACAA+AGG 0.556469 8.4:+26258064 MS.gene99219:CDS
TTAGACGGTGATAGCATCCA+TGG 0.565051 8.4:-26258789 None:intergenic
ATACTTTGGTTGAAGGATGT+CGG 0.566713 8.4:-26257784 None:intergenic
TGGGATTGGTAAAATTGCAA+TGG 0.567200 8.4:+26258451 MS.gene99219:CDS
ATTCTTGTCCTACAATCTAC+GGG 0.570480 8.4:-26258341 None:intergenic
AACGAAGTGCAAAACTATGA+AGG 0.575652 8.4:+26257840 MS.gene99219:CDS
GTGCTTGGTGGTGCAGAAGA+AGG 0.576706 8.4:+26258701 MS.gene99219:CDS
TGGTTGAAGGATGTCGGCAA+TGG 0.578913 8.4:-26257778 None:intergenic
TCCATCATTTGCAACAACGC+TGG 0.580870 8.4:+26257720 MS.gene99219:CDS
TTCGTTGAGTAAGAATAATG+AGG 0.583239 8.4:-26257823 None:intergenic
CTACCTGGTTCCGATATAGA+CGG 0.584378 8.4:-26258548 None:intergenic
TGGAAAGAAGTTGAGAGTGT+TGG 0.586632 8.4:-26257640 None:intergenic
TTTGTGAAAGGAAACTGTAT+GGG 0.590779 8.4:-26258062 None:intergenic
GGGAAATGATCATCTCTCCA+TGG 0.596430 8.4:+26258772 MS.gene99219:CDS
ACAATCCGAGCAACAAAACA+TGG 0.599813 8.4:+26257480 MS.gene99219:CDS
AAATTACTTGATGATCTACC+TGG 0.604805 8.4:-26258563 None:intergenic
GATAAGTAGAATGCCTGCCA+TGG 0.611905 8.4:-26257448 None:intergenic
ACCCCGTCTATATCGGAACC+AGG 0.621903 8.4:+26258545 MS.gene99219:CDS
ACCGATCTGCAAGTATTAGA+CGG 0.621985 8.4:-26258804 None:intergenic
TCTTGGGCTGAAATCGCACA+AGG 0.627018 8.4:+26257978 MS.gene99219:CDS
AATAATGAGGAAACAATAGG+AGG 0.628636 8.4:-26257810 None:intergenic
ACAAGACGACCAGCGAGTGG+TGG 0.630223 8.4:-26257660 None:intergenic
AATTGCAATGGAAATGAACA+AGG 0.638167 8.4:+26258463 MS.gene99219:CDS
TAGTGTGTTCAGCTGTGGCG+TGG 0.644539 8.4:-26257755 None:intergenic
AAATTATCGATTTAGCACCA+TGG 0.647404 8.4:+26257523 MS.gene99219:CDS
ATTTGGCGTCTACACCCACA+TGG 0.667304 8.4:-26257935 None:intergenic
AATGGTAGTGTGTTCAGCTG+TGG 0.672501 8.4:-26257760 None:intergenic
CAAGTAACAGAGTTAATCGA+CGG 0.676652 8.4:+26257888 MS.gene99219:CDS
ACCTGGTTCCGATATAGACG+GGG 0.680926 8.4:-26258546 None:intergenic
ATTACAAGACGACCAGCGAG+TGG 0.695572 8.4:-26257663 None:intergenic
GTTGCAAATGATGGAACATG+AGG 0.705156 8.4:-26257712 None:intergenic
TGATAGCAAGCAGTTCACCA+CGG 0.707603 8.4:+26258588 MS.gene99219:CDS
TTACCGTAATAATTGAACCG+TGG 0.738497 8.4:-26258605 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTCTCTTCTAACTT+TGG - chr8.4:26258371-26258390 None:intergenic 20.0%
!! GTTGGATTTAAAAGAAATAT+TGG + chr8.4:26258668-26258687 MS.gene99219:CDS 20.0%
!!! AAGTTAGAAGAGAATTTTTT+TGG + chr8.4:26258371-26258390 MS.gene99219:CDS 20.0%
! AAGAATAATGAGGAAACAAT+AGG - chr8.4:26257816-26257835 None:intergenic 25.0%
! AATTATTACGGTAATGACTT+TGG + chr8.4:26258614-26258633 MS.gene99219:CDS 25.0%
! GATATGTAATAATGCAAGTA+GGG + chr8.4:26258414-26258433 MS.gene99219:CDS 25.0%
! GGATCTCAAATATCTTAAAA+TGG + chr8.4:26257545-26257564 MS.gene99219:CDS 25.0%
! TACTTATAATTAAACGCCAA+AGG - chr8.4:26258280-26258299 None:intergenic 25.0%
!!! AATTTTTTTGGAAGTGGAAT+CGG + chr8.4:26258383-26258402 MS.gene99219:CDS 25.0%
AAAATCTTACACCAGAAGAA+AGG + chr8.4:26258303-26258322 MS.gene99219:CDS 30.0%
AAATTAATGGAAAGTGGGAT+TGG + chr8.4:26258437-26258456 MS.gene99219:CDS 30.0%
AATAATGAGGAAACAATAGG+AGG - chr8.4:26257813-26257832 None:intergenic 30.0%
AATGCAAGTAGGGAAATTAA+TGG + chr8.4:26258424-26258443 MS.gene99219:CDS 30.0%
AATTGCAATGGAAATGAACA+AGG + chr8.4:26258463-26258482 MS.gene99219:CDS 30.0%
GATGAAATGCCAAAATGATT+TGG - chr8.4:26257955-26257974 None:intergenic 30.0%
GGATATGTAATAATGCAAGT+AGG + chr8.4:26258413-26258432 MS.gene99219:CDS 30.0%
TAGGGAAATTAATGGAAAGT+GGG + chr8.4:26258432-26258451 MS.gene99219:CDS 30.0%
TAGTGAGTAATTATGAGTCT+TGG + chr8.4:26258516-26258535 MS.gene99219:CDS 30.0%
TCAAAAGCTAATGCAGAAAT+TGG + chr8.4:26258191-26258210 MS.gene99219:CDS 30.0%
TTCGTTGAGTAAGAATAATG+AGG - chr8.4:26257826-26257845 None:intergenic 30.0%
TTGTCTTCCTTATGATACTT+TGG + chr8.4:26258742-26258761 MS.gene99219:CDS 30.0%
TTTGTGAAAGGAAACTGTAT+GGG - chr8.4:26258065-26258084 None:intergenic 30.0%
! AAAAGAAGACCTTTTTGGAT+TGG - chr8.4:26257573-26257592 None:intergenic 30.0%
! AAATTACTTGATGATCTACC+TGG - chr8.4:26258566-26258585 None:intergenic 30.0%
! GAATTTGTTCCTTAGTAAAG+TGG - chr8.4:26258160-26258179 None:intergenic 30.0%
! TATGGAAAAGAAGACCTTTT+TGG - chr8.4:26257578-26257597 None:intergenic 30.0%
! TTAAGATATTTGAGATCCCA+TGG - chr8.4:26257543-26257562 None:intergenic 30.0%
! TTGAAAGAAGGGATTTTTGT+TGG - chr8.4:26258014-26258033 None:intergenic 30.0%
! TTTGTTTAAGATGGTCGATT+TGG - chr8.4:26257614-26257633 None:intergenic 30.0%
! TTTTGTGCTTCATTTGTGAA+AGG - chr8.4:26258104-26258123 None:intergenic 30.0%
!! AAATTATCGATTTAGCACCA+TGG + chr8.4:26257523-26257542 MS.gene99219:CDS 30.0%
!! AATTATCGATTTAGCACCAT+GGG + chr8.4:26257524-26257543 MS.gene99219:CDS 30.0%
!! TGGAAAGTGTTTGTTTAAGA+TGG - chr8.4:26257623-26257642 None:intergenic 30.0%
!!! GAAGAGAATTTTTTTGGAAG+TGG + chr8.4:26258377-26258396 MS.gene99219:CDS 30.0%
!!! TTTGGTTTGTTTTGGGTTTA+TGG - chr8.4:26257596-26257615 None:intergenic 30.0%
AAAATGGCACCAATCCAAAA+AGG + chr8.4:26257561-26257580 MS.gene99219:CDS 35.0%
AAATCCCTTCTTTCAAACGT+TGG + chr8.4:26258018-26258037 MS.gene99219:CDS 35.0%
AACGAAGTGCAAAACTATGA+AGG + chr8.4:26257840-26257859 MS.gene99219:CDS 35.0%
AAGCCTATTGCAGTTAGAAA+TGG + chr8.4:26258644-26258663 MS.gene99219:CDS 35.0%
ACAATAGGAGGAACATACTT+TGG - chr8.4:26257801-26257820 None:intergenic 35.0%
ACACCATTTCTAACTGCAAT+AGG - chr8.4:26258650-26258669 None:intergenic 35.0%
ATACTTTGGTTGAAGGATGT+CGG - chr8.4:26257787-26257806 None:intergenic 35.0%
ATGGGACGATTAATGTTGTT+TGG - chr8.4:26258047-26258066 None:intergenic 35.0%
ATTACGGTAATGACTTTGGT+TGG + chr8.4:26258618-26258637 MS.gene99219:CDS 35.0%
ATTCTTGTCCTACAATCTAC+GGG - chr8.4:26258344-26258363 None:intergenic 35.0%
ATTGCAGTTAGAAATGGTGT+TGG + chr8.4:26258650-26258669 MS.gene99219:CDS 35.0%
ATTGGTAGAGTTATTGTGCT+TGG + chr8.4:26258686-26258705 MS.gene99219:CDS 35.0%
CAAGTAACAGAGTTAATCGA+CGG + chr8.4:26257888-26257907 MS.gene99219:CDS 35.0%
CATACAGTTTCCTTTCACAA+AGG + chr8.4:26258064-26258083 MS.gene99219:CDS 35.0%
CATTCTTGTCCTACAATCTA+CGG - chr8.4:26258345-26258364 None:intergenic 35.0%
CTTTGTGAAAGGAAACTGTA+TGG - chr8.4:26258066-26258085 None:intergenic 35.0%
GAAACCAACGTTTGAAAGAA+GGG - chr8.4:26258025-26258044 None:intergenic 35.0%
GTAGGGAAATTAATGGAAAG+TGG + chr8.4:26258431-26258450 MS.gene99219:CDS 35.0%
GTTACTTGTACTGCAAGTAA+TGG - chr8.4:26257876-26257895 None:intergenic 35.0%
TCACCACGGTTCAATTATTA+CGG + chr8.4:26258602-26258621 MS.gene99219:CDS 35.0%
TCGAATCTTCCACTTTACTA+AGG + chr8.4:26258148-26258167 MS.gene99219:CDS 35.0%
TCTTCCTTATGATACTTTGG+AGG + chr8.4:26258745-26258764 MS.gene99219:CDS 35.0%
TTAATCGACGGTATCTTCAT+TGG + chr8.4:26257900-26257919 MS.gene99219:CDS 35.0%
TTACCGTAATAATTGAACCG+TGG - chr8.4:26258608-26258627 None:intergenic 35.0%
TTACGGTAATGACTTTGGTT+GGG + chr8.4:26258619-26258638 MS.gene99219:CDS 35.0%
TTGGCATTTCATCAACTCTT+GGG + chr8.4:26257962-26257981 MS.gene99219:CDS 35.0%
TTTGGCATTTCATCAACTCT+TGG + chr8.4:26257961-26257980 MS.gene99219:CDS 35.0%
! ATAGCCTCCAAAGTATCATA+AGG - chr8.4:26258752-26258771 None:intergenic 35.0%
! GGTTTTGCAATTAACCATGT+GGG + chr8.4:26257921-26257940 MS.gene99219:CDS 35.0%
! TATGATACTTTGGAGGCTAT+GGG + chr8.4:26258752-26258771 MS.gene99219:CDS 35.0%
! TGGGATTGGTAAAATTGCAA+TGG + chr8.4:26258451-26258470 MS.gene99219:CDS 35.0%
! TTATGATACTTTGGAGGCTA+TGG + chr8.4:26258751-26258770 MS.gene99219:CDS 35.0%
! TTTTGTGCTTCCTTTGTGAA+AGG - chr8.4:26258077-26258096 None:intergenic 35.0%
!! AAGCACTTTTAAGTCACCTT+TGG + chr8.4:26258261-26258280 MS.gene99219:CDS 35.0%
!! TGGTTTTGCAATTAACCATG+TGG + chr8.4:26257920-26257939 MS.gene99219:CDS 35.0%
!!! ATGGTCGATTTGGTTTGTTT+TGG - chr8.4:26257604-26257623 None:intergenic 35.0%
!!! TGGTCGATTTGGTTTGTTTT+GGG - chr8.4:26257603-26257622 None:intergenic 35.0%
ACAATCCGAGCAACAAAACA+TGG + chr8.4:26257480-26257499 MS.gene99219:CDS 40.0%
GAGGAACATACTTTGGTTGA+AGG - chr8.4:26257794-26257813 None:intergenic 40.0%
GGAAACCAACGTTTGAAAGA+AGG - chr8.4:26258026-26258045 None:intergenic 40.0%
GTTGAAAACCCCGTCTATAT+CGG + chr8.4:26258538-26258557 MS.gene99219:CDS 40.0%
TACGGTAATGACTTTGGTTG+GGG + chr8.4:26258620-26258639 MS.gene99219:CDS 40.0%
! GTGTAGACGCCAAATCATTT+TGG + chr8.4:26257943-26257962 MS.gene99219:CDS 40.0%
! GTTGCAAATGATGGAACATG+AGG - chr8.4:26257715-26257734 None:intergenic 40.0%
! TGGAAAGAAGTTGAGAGTGT+TGG - chr8.4:26257643-26257662 None:intergenic 40.0%
!! CATCACCATGTTTTGTTGCT+CGG - chr8.4:26257488-26257507 None:intergenic 40.0%
ACCAGCGTTGTTGCAAATGA+TGG - chr8.4:26257724-26257743 None:intergenic 45.0%
ACGAAAGCAGACCTTTCTTC+TGG - chr8.4:26258317-26258336 None:intergenic 45.0%
CTACCTGGTTCCGATATAGA+CGG - chr8.4:26258551-26258570 None:intergenic 45.0%
GGGAAATGATCATCTCTCCA+TGG + chr8.4:26258772-26258791 MS.gene99219:CDS 45.0%
GGTAGAGTTATTGTGCTTGG+TGG + chr8.4:26258689-26258708 MS.gene99219:CDS 45.0%
TACCTGGTTCCGATATAGAC+GGG - chr8.4:26258550-26258569 None:intergenic 45.0%
TCCATCATTTGCAACAACGC+TGG + chr8.4:26257720-26257739 MS.gene99219:CDS 45.0%
TCGTGTTACCCGTAGATTGT+AGG + chr8.4:26258333-26258352 MS.gene99219:CDS 45.0%
TGATAGCAAGCAGTTCACCA+CGG + chr8.4:26258588-26258607 MS.gene99219:CDS 45.0%
TTAGACGGTGATAGCATCCA+TGG - chr8.4:26258792-26258811 None:intergenic 45.0%
! AATGGTAGTGTGTTCAGCTG+TGG - chr8.4:26257763-26257782 None:intergenic 45.0%
!! ATGGAACATGAGGCGTTGTT+AGG - chr8.4:26257705-26257724 None:intergenic 45.0%
AACTTCTTTCCACCACTCGC+TGG + chr8.4:26257651-26257670 MS.gene99219:CDS 50.0%
ACCTGGTTCCGATATAGACG+GGG - chr8.4:26258549-26258568 None:intergenic 50.0%
ATTACAAGACGACCAGCGAG+TGG - chr8.4:26257666-26257685 None:intergenic 50.0%
ATTTGGCGTCTACACCCACA+TGG - chr8.4:26257938-26257957 None:intergenic 50.0%
TCTTGGGCTGAAATCGCACA+AGG + chr8.4:26257978-26257997 MS.gene99219:CDS 50.0%
TGGTTGAAGGATGTCGGCAA+TGG - chr8.4:26257781-26257800 None:intergenic 50.0%
ACCCCGTCTATATCGGAACC+AGG + chr8.4:26258545-26258564 MS.gene99219:CDS 55.0%
GGAAGTGGAATCGGAGCAAC+TGG + chr8.4:26258392-26258411 MS.gene99219:CDS 55.0%
TAGTGTGTTCAGCTGTGGCG+TGG - chr8.4:26257758-26257777 None:intergenic 55.0%
!! GTGCTTGGTGGTGCAGAAGA+AGG + chr8.4:26258701-26258720 MS.gene99219:CDS 55.0%
ACAAGACGACCAGCGAGTGG+TGG - chr8.4:26257663-26257682 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 26257450 26258811 26257450 ID=MS.gene99219
chr8.4 mRNA 26257450 26258811 26257450 ID=MS.gene99219.t1;Parent=MS.gene99219
chr8.4 exon 26257450 26258811 26257450 ID=MS.gene99219.t1.exon1;Parent=MS.gene99219.t1
chr8.4 CDS 26257450 26258811 26257450 ID=cds.MS.gene99219.t1;Parent=MS.gene99219.t1
Gene Sequence

>MS.gene99219

ATGGCAGGCATTCTACTTATCTCGACTACTACAATCCGAGCAACAAAACATGGTGATGACAACTCAACTCACAAAATTATCGATTTAGCACCATGGGATCTCAAATATCTTAAAATGGCACCAATCCAAAAAGGTCTTCTTTTCCATAAACCCAAAACAAACCAAATCGACCATCTTAAACAAACACTTTCCAACACTCTCAACTTCTTTCCACCACTCGCTGGTCGTCTTGTAATAACACAACACGACGAACCTAACAACGCCTCATGTTCCATCATTTGCAACAACGCTGGTGCATTGTTTGTCCACGCCACAGCTGAACACACTACCATTGCCGACATCCTTCAACCAAAGTATGTTCCTCCTATTGTTTCCTCATTATTCTTACTCAACGAAGTGCAAAACTATGAAGGCACATCACAACCATTACTTGCAGTACAAGTAACAGAGTTAATCGACGGTATCTTCATTGGTTTTGCAATTAACCATGTGGGTGTAGACGCCAAATCATTTTGGCATTTCATCAACTCTTGGGCTGAAATCGCACAAGGTTTCAATAAGCCAACAAAAATCCCTTCTTTCAAACGTTGGTTTCCAAACAACATTAATCGTCCCATACAGTTTCCTTTCACAAAGGAAGCACAAAAGCAGCCTTTCACAAATGAAGCACAAAATCAACAATGTGAAGTTTCGTCTCATCGAATCTTCCACTTTACTAAGGAACAAATTCTGCAACTCAAATCAAAAGCTAATGCAGAAATTGGTAGTAGTAGTACTAGTAGTGATAAAATAATAACAATATCGTCATTGCAAGCACTTTTAAGTCACCTTTGGCGTTTAATTATAAGTAAACAAAATCTTACACCAGAAGAAAGGTCTGCTTTCGTGTTACCCGTAGATTGTAGGACAAGAATGTGTCCAAAGTTAGAAGAGAATTTTTTTGGAAGTGGAATCGGAGCAACTGGATATGTAATAATGCAAGTAGGGAAATTAATGGAAAGTGGGATTGGTAAAATTGCAATGGAAATGAACAAGGAGATTTCATCAAAATCTCATCATGAAAAGATAGTGAGTAATTATGAGTCTTGGTTGAAAACCCCGTCTATATCGGAACCAGGTAGATCATCAAGTAATTTGTTGATAGCAAGCAGTTCACCACGGTTCAATTATTACGGTAATGACTTTGGTTGGGGTAAGCCTATTGCAGTTAGAAATGGTGTTGGATTTAAAAGAAATATTGGTAGAGTTATTGTGCTTGGTGGTGCAGAAGAAGGTAGTATTGATATTCAAGTTTGTCTTCCTTATGATACTTTGGAGGCTATGGGAAATGATCATCTCTCCATGGATGCTATCACCGTCTAA
Protein sequence

>MS.gene99219.t1

MAGILLISTTTIRATKHGDDNSTHKIIDLAPWDLKYLKMAPIQKGLLFHKPKTNQIDHLKQTLSNTLNFFPPLAGRLVITQHDEPNNASCSIICNNAGALFVHATAEHTTIADILQPKYVPPIVSSLFLLNEVQNYEGTSQPLLAVQVTELIDGIFIGFAINHVGVDAKSFWHFINSWAEIAQGFNKPTKIPSFKRWFPNNINRPIQFPFTKEAQKQPFTNEAQNQQCEVSSHRIFHFTKEQILQLKSKANAEIGSSSTSSDKIITISSLQALLSHLWRLIISKQNLTPEERSAFVLPVDCRTRMCPKLEENFFGSGIGATGYVIMQVGKLMESGIGKIAMEMNKEISSKSHHEKIVSNYESWLKTPSISEPGRSSSNLLIASSSPRFNYYGNDFGWGKPIAVRNGVGFKRNIGRVIVLGGAEEGSIDIQVCLPYDTLEAMGNDHLSMDAITV