AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99223

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99223.t1 MTR_4g094842 91.971 411 30 2 1 410 39 447 0.0 773
MS.gene99223.t1 MTR_4g094835 61.463 410 140 7 3 407 46 442 7.04e-178 503
MS.gene99223.t1 MTR_8g041770 58.293 410 158 5 1 409 42 439 1.98e-165 471
MS.gene99223.t1 MTR_4g022825 57.831 415 155 7 2 409 37 438 2.30e-164 469
MS.gene99223.t1 MTR_4g094838 59.367 411 137 7 3 407 42 428 1.60e-163 466
MS.gene99223.t1 MTR_4g094832 64.225 355 115 5 58 407 79 426 7.21e-163 464
MS.gene99223.t1 MTR_0190s0020 55.257 409 174 4 1 409 41 440 3.14e-161 461
MS.gene99223.t1 MTR_4g022720 58.738 412 151 8 2 409 37 433 2.84e-160 458
MS.gene99223.t1 MTR_4g022800 57.416 418 154 8 2 409 37 440 1.08e-159 457
MS.gene99223.t1 MTR_3g088420 57.561 410 155 8 3 409 38 431 3.09e-159 456
MS.gene99223.t1 MTR_4g022670 56.835 417 157 8 2 409 37 439 1.35e-158 454
MS.gene99223.t1 MTR_8g079710 57.664 411 146 9 3 402 43 436 1.86e-158 454
MS.gene99223.t1 MTR_7g026860 56.731 416 156 8 2 409 37 436 1.34e-156 449
MS.gene99223.t1 MTR_3g026310 55.107 421 161 8 3 408 38 445 1.86e-154 444
MS.gene99223.t1 MTR_4g094828 54.126 412 166 8 3 403 43 442 2.85e-154 444
MS.gene99223.t1 MTR_4g022290 54.106 414 159 9 3 404 44 438 8.70e-150 432
MS.gene99223.t1 MTR_4g022760 51.923 416 135 7 2 409 37 395 3.98e-135 393
MS.gene99223.t1 MTR_3g101900 42.788 416 212 13 3 407 44 444 8.98e-100 304
MS.gene99223.t1 MTR_3g101930 42.654 422 215 11 3 409 44 453 1.15e-98 302
MS.gene99223.t1 MTR_6g016815 44.359 390 140 8 19 407 64 377 1.58e-96 294
MS.gene99223.t1 MTR_3g101960 40.964 415 222 10 3 406 43 445 7.04e-90 279
MS.gene99223.t1 MTR_3g101970 40.821 414 215 11 2 407 42 433 2.51e-88 275
MS.gene99223.t1 MTR_3g102020 40.048 417 226 11 3 407 43 447 6.42e-87 271
MS.gene99223.t1 MTR_3g102010 38.702 416 232 10 3 407 44 447 2.16e-82 260
MS.gene99223.t1 MTR_3g070170 35.892 443 223 9 3 407 47 466 3.30e-82 260
MS.gene99223.t1 MTR_8g040780 54.419 215 95 2 195 406 13 227 1.19e-78 243
MS.gene99223.t1 MTR_5g081960 33.190 464 229 11 3 407 41 482 4.31e-72 234
MS.gene99223.t1 MTR_4g015180 33.405 467 227 10 3 408 41 484 1.37e-70 230
MS.gene99223.t1 MTR_7g026850 61.039 154 48 3 2 154 20 162 2.23e-58 189
MS.gene99223.t1 MTR_8g040760 57.923 183 68 5 2 183 40 214 3.37e-58 189
MS.gene99223.t1 MTR_6g045553 32.579 442 244 12 2 407 48 471 1.96e-57 195
MS.gene99223.t1 MTR_4g094825 54.335 173 72 3 3 175 106 271 4.99e-54 184
MS.gene99223.t1 MTR_4g094825 58.278 151 51 2 258 407 248 387 1.16e-51 178
MS.gene99223.t1 MTR_7g026770 49.485 194 72 2 116 307 132 301 1.82e-49 169
MS.gene99223.t1 MTR_3g102030 34.615 338 180 11 35 365 17 320 5.28e-48 166
MS.gene99223.t1 MTR_6g034495 61.983 121 43 1 33 153 43 160 3.05e-46 157
MS.gene99223.t1 MTR_6g034485 56.250 128 52 2 161 288 1 124 1.83e-44 150
MS.gene99223.t1 MTR_4g007540 27.619 420 246 16 8 398 34 424 3.53e-37 140
MS.gene99223.t1 MTR_2g064495 27.361 413 255 14 8 398 34 423 2.35e-35 135
MS.gene99223.t1 MTR_2g105330 27.361 413 255 14 8 398 34 423 3.78e-35 134
MS.gene99223.t1 MTR_8g075610 27.179 390 246 14 24 399 47 412 2.11e-33 129
MS.gene99223.t1 MTR_7g026830 62.222 90 34 0 51 140 8 97 5.15e-33 120
MS.gene99223.t1 MTR_8g096530 30.484 351 207 12 22 360 57 382 7.42e-33 129
MS.gene99223.t1 MTR_3g098980 28.382 377 238 12 24 392 66 418 5.31e-31 123
MS.gene99223.t1 MTR_3g026950 27.869 427 235 15 12 396 42 437 8.48e-30 120
MS.gene99223.t1 MTR_4g127650 25.776 419 255 17 6 392 46 440 5.32e-25 106
MS.gene99223.t1 MTR_1g081660 24.934 381 242 13 8 374 57 407 9.15e-25 105
MS.gene99223.t1 MTR_4g013050 27.184 412 225 12 15 402 44 404 2.51e-24 104
MS.gene99223.t1 MTR_4g097090 25.647 425 246 15 7 392 47 440 2.91e-24 104
MS.gene99223.t1 MTR_3g049150 26.733 404 246 15 21 396 52 433 5.36e-24 103
MS.gene99223.t1 MTR_7g027175 25.888 394 259 12 15 398 59 429 5.42e-23 100
MS.gene99223.t1 MTR_7g014250 27.363 402 233 16 7 374 53 429 5.71e-23 100
MS.gene99223.t1 MTR_7g014330 27.154 383 228 15 24 372 62 427 3.69e-22 98.6
MS.gene99223.t1 MTR_1g040195 27.273 352 222 14 64 398 71 405 4.08e-22 98.2
MS.gene99223.t1 MTR_2g089765 25.672 409 251 16 24 395 75 467 2.72e-21 95.9
MS.gene99223.t1 MTR_7g014230 27.937 383 225 15 24 372 64 429 4.63e-21 95.1
MS.gene99223.t1 MTR_7g085930 24.036 441 257 14 6 398 41 451 6.80e-21 94.7
MS.gene99223.t1 MTR_7g084940 28.255 361 218 15 15 360 58 392 7.44e-21 94.4
MS.gene99223.t1 MTR_7g026780 44.037 109 36 3 301 409 26 109 1.87e-20 86.7
MS.gene99223.t1 MTR_6g015315 27.653 311 198 11 113 401 140 445 2.03e-20 93.2
MS.gene99223.t1 MTR_1g040175 24.791 359 240 12 24 375 64 399 4.58e-20 92.0
MS.gene99223.t1 MTR_3g071320 26.710 307 190 11 6 295 42 330 8.10e-20 91.3
MS.gene99223.t1 MTR_1g040215 25.410 366 229 15 24 375 64 399 8.82e-20 91.3
MS.gene99223.t1 MTR_6g015320 26.059 307 201 10 113 398 143 444 1.18e-19 90.9
MS.gene99223.t1 MTR_6g015850 26.710 307 200 8 113 399 147 448 1.84e-19 90.5
MS.gene99223.t1 MTR_6g015815 25.641 312 202 10 113 400 147 452 2.35e-19 90.1
MS.gene99223.t1 MTR_3g086020 24.190 401 243 14 6 392 43 396 3.09e-19 89.4
MS.gene99223.t1 MTR_4g055170 27.362 307 189 10 14 308 59 343 3.37e-19 89.7
MS.gene99223.t1 MTR_3g087640 26.634 413 248 16 24 398 67 462 6.58e-19 89.0
MS.gene99223.t1 MTR_7g015860 25.176 425 247 16 24 403 64 462 1.51e-18 87.8
MS.gene99223.t1 MTR_6g015830 25.890 309 202 8 113 399 148 451 1.74e-18 87.4
MS.gene99223.t1 MTR_3g465980 26.599 297 202 10 1 291 43 329 4.28e-18 86.3
MS.gene99223.t1 MTR_7g085990 23.928 443 259 15 6 400 37 449 5.29e-18 85.9
MS.gene99223.t1 MTR_6g053810 25.979 281 192 8 18 292 65 335 6.45e-18 85.9
MS.gene99223.t1 MTR_3g028210 23.821 403 266 13 17 392 60 448 7.48e-18 85.5
MS.gene99223.t1 MTR_1g053315 24.217 351 235 15 24 361 78 410 1.02e-17 85.1
MS.gene99223.t1 MTR_5g076700 22.760 413 275 16 2 392 36 426 1.64e-17 84.7
MS.gene99223.t1 MTR_7g026800 37.143 140 58 2 189 327 27 137 2.06e-17 80.5
MS.gene99223.t1 MTR_7g086000 26.566 399 246 15 24 392 60 441 2.71e-17 84.0
MS.gene99223.t1 MTR_8g012750 25.936 374 227 13 24 360 62 422 6.66e-17 82.8
MS.gene99223.t1 MTR_7g114550 23.724 392 257 15 2 374 36 404 6.91e-17 82.8
MS.gene99223.t1 MTR_3g049140 26.957 230 133 7 185 396 18 230 9.32e-17 79.7
MS.gene99223.t1 MTR_5g090830 26.448 397 221 15 7 361 45 412 1.27e-16 82.0
MS.gene99223.t1 MTR_7g114470 23.877 423 259 18 2 392 37 428 1.37e-16 81.6
MS.gene99223.t1 MTR_7g114560 23.724 392 257 15 2 374 36 404 1.53e-16 81.6
MS.gene99223.t1 MTR_5g090810 25.693 397 224 15 7 361 45 412 1.58e-16 81.6
MS.gene99223.t1 MTR_8g079980 23.020 404 232 14 6 377 41 397 2.12e-16 80.9
MS.gene99223.t1 MTR_5g084850 23.907 389 241 16 6 377 41 391 2.75e-16 80.9
MS.gene99223.t1 MTR_2g089670 27.596 366 218 16 24 360 82 429 3.20e-16 80.9
MS.gene99223.t1 MTR_8g079970 22.278 395 247 13 6 377 41 398 4.14e-16 80.1
MS.gene99223.t1 MTR_2g089755 24.468 376 232 13 24 363 73 432 5.27e-16 80.1
MS.gene99223.t1 MTR_7g014360 27.372 274 155 11 113 363 90 342 5.58e-16 79.3
MS.gene99223.t1 MTR_7g114520 23.604 394 262 15 9 386 53 423 6.08e-16 79.7
MS.gene99223.t1 MTR_3g465990 27.083 288 187 12 13 291 56 329 6.58e-16 79.7
MS.gene99223.t1 MTR_5g026320 23.487 413 254 14 21 406 71 448 7.13e-16 79.7
MS.gene99223.t1 MTR_7g014160 26.277 411 237 18 24 392 69 455 2.61e-15 77.8
MS.gene99223.t1 MTR_3g450710 24.235 392 234 15 7 361 46 411 3.52e-15 77.4
MS.gene99223.t1 MTR_5g084870 21.883 393 245 14 6 377 41 392 4.00e-15 77.0
MS.gene99223.t1 MTR_7g013850 20.833 432 266 12 19 398 63 470 4.14e-15 77.4
MS.gene99223.t1 MTR_7g084910 26.633 398 231 18 15 393 59 414 1.35e-14 75.5
MS.gene99223.t1 MTR_5g090880 24.227 388 238 15 8 361 49 414 1.86e-14 75.1
MS.gene99223.t1 MTR_7g056087 22.816 412 273 15 8 398 42 429 2.40e-14 74.7
MS.gene99223.t1 MTR_8g079950 23.750 400 249 15 6 384 41 405 4.03e-14 74.3
MS.gene99223.t1 MTR_3g465930 25.000 280 192 6 19 291 64 332 1.96e-13 72.0
MS.gene99223.t1 MTR_1g094790 24.700 417 258 16 16 407 52 437 1.13e-12 69.7
MS.gene99223.t1 MTR_1g040315 25.253 297 177 12 2 279 35 305 1.66e-12 68.9
MS.gene99223.t1 MTR_2g015430 22.409 357 244 11 24 369 58 392 2.69e-12 68.6
MS.gene99223.t1 MTR_7g026880 51.923 52 23 1 358 409 2 51 3.12e-12 61.6
MS.gene99223.t1 MTR_3g465910 25.979 281 186 9 19 291 63 329 1.14e-11 66.6
MS.gene99223.t1 MTR_7g014620 24.149 323 176 9 113 398 132 422 1.47e-11 66.2
MS.gene99223.t1 MTR_7g026870 53.704 54 25 0 267 320 98 151 1.76e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99223.t1 AT5G67150 42.995 414 213 13 3 407 43 442 2.27e-103 313
MS.gene99223.t1 AT3G50270 40.049 412 230 10 3 407 43 444 1.25e-91 283
MS.gene99223.t1 AT3G50280 39.320 412 230 9 3 407 41 439 7.15e-91 281
MS.gene99223.t1 AT3G50300 41.791 402 212 11 3 396 41 428 1.09e-90 281
MS.gene99223.t1 AT5G67160 40.342 409 215 11 3 406 43 427 1.04e-87 273
MS.gene99223.t1 AT5G38130 40.250 400 213 12 3 393 61 443 1.79e-86 271
MS.gene99223.t1 AT5G07860 39.036 415 233 9 3 409 45 447 1.80e-84 265
MS.gene99223.t1 AT5G07850 38.995 418 233 9 3 409 45 451 4.58e-84 264
MS.gene99223.t1 AT5G42830 38.129 417 235 10 3 409 44 447 1.22e-83 263
MS.gene99223.t1 AT5G07870 38.228 429 229 12 3 409 45 459 1.08e-81 258
MS.gene99223.t1 AT2G39980 34.286 455 230 8 3 408 43 477 3.89e-79 252
MS.gene99223.t1 AT5G01210 35.360 444 231 7 3 408 41 466 3.95e-78 249
MS.gene99223.t1 AT3G50280 38.339 313 184 4 100 407 1 309 5.92e-69 221
MS.gene99223.t1 AT5G23940 32.787 427 250 10 2 407 38 448 3.08e-65 216
MS.gene99223.t1 AT3G50290 42.963 270 142 7 97 358 4 269 2.72e-58 192
MS.gene99223.t1 AT5G48930 28.607 402 253 14 8 392 34 418 1.35e-36 139
MS.gene99223.t1 AT3G48720 25.815 399 271 9 7 399 44 423 6.04e-28 114
MS.gene99223.t1 AT2G19070 25.532 423 268 14 6 400 40 443 6.81e-27 112
MS.gene99223.t1 AT5G41040 27.696 408 244 17 21 409 66 441 6.54e-26 109
MS.gene99223.t1 AT5G41040 27.696 408 244 17 21 409 82 457 7.58e-26 109
MS.gene99223.t1 AT5G57840 27.446 419 247 17 1 392 34 422 8.21e-25 105
MS.gene99223.t1 AT5G63560 25.189 397 258 11 7 392 44 412 1.26e-23 102
MS.gene99223.t1 AT3G29590 26.646 319 194 12 110 402 138 442 2.86e-23 101
MS.gene99223.t1 AT5G17540 26.972 393 237 17 2 372 35 399 1.45e-22 99.8
MS.gene99223.t1 AT2G40230 25.915 355 240 9 23 370 58 396 1.56e-22 99.4
MS.gene99223.t1 AT3G29635 24.936 393 250 12 24 392 67 438 2.81e-22 99.0
MS.gene99223.t1 AT1G03940 25.000 408 252 16 21 395 63 449 3.18e-22 98.6
MS.gene99223.t1 AT3G03480 27.114 402 235 17 2 380 45 411 3.24e-22 98.6
MS.gene99223.t1 AT5G61160 25.949 316 191 12 110 398 139 438 7.00e-21 94.7
MS.gene99223.t1 AT5G07080 27.393 303 185 12 4 291 49 331 1.63e-20 93.6
MS.gene99223.t1 AT1G65450 26.837 313 198 13 64 360 103 400 3.95e-19 89.4
MS.gene99223.t1 AT1G24420 25.389 386 243 16 34 408 75 426 5.46e-19 89.0
MS.gene99223.t1 AT1G24420 25.389 386 243 16 34 408 75 426 5.46e-19 89.0
MS.gene99223.t1 AT1G24430 24.872 390 245 16 6 384 45 397 1.09e-18 88.2
MS.gene99223.t1 AT3G47170 28.873 284 169 12 28 291 74 344 1.73e-18 87.4
MS.gene99223.t1 AT5G16410 26.385 379 236 12 24 384 91 444 3.33e-18 86.7
MS.gene99223.t1 AT5G39050 24.252 301 187 11 124 393 160 450 6.99e-18 85.9
MS.gene99223.t1 AT1G32910 25.798 376 224 16 12 361 53 399 2.47e-17 84.0
MS.gene99223.t1 AT5G39080 24.566 403 246 17 21 388 62 441 1.39e-16 81.6
MS.gene99223.t1 AT3G26040 24.113 423 264 15 6 408 41 426 1.81e-16 81.3
MS.gene99223.t1 AT1G03390 24.523 367 247 14 7 361 59 407 2.32e-16 81.3
MS.gene99223.t1 AT3G30280 23.671 414 234 16 5 384 43 408 4.10e-16 80.1
MS.gene99223.t1 AT5G23970 25.990 404 227 19 16 392 44 402 5.49e-16 79.7
MS.gene99223.t1 AT3G29680 25.681 257 164 9 124 361 152 400 1.12e-15 79.0
MS.gene99223.t1 AT1G28680 24.501 351 225 14 27 363 65 389 1.27e-15 79.0
MS.gene99223.t1 AT5G47980 26.044 407 234 22 6 384 41 408 4.67e-15 77.0
MS.gene99223.t1 AT1G28680 25.163 306 194 12 27 320 65 347 1.33e-14 75.5
MS.gene99223.t1 AT4G15390 22.196 428 271 15 5 407 45 435 3.04e-14 74.7
MS.gene99223.t1 AT4G15390 22.196 428 271 15 5 407 45 435 3.04e-14 74.7
MS.gene99223.t1 AT1G65450 26.939 245 159 9 127 360 1 236 9.17e-14 71.6
MS.gene99223.t1 AT2G23510 23.698 384 268 16 4 378 48 415 5.65e-13 70.9

Find 71 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCAAGGTTTGATGTTTA+TGG 0.150931 8.4:+26219402 MS.gene99223:CDS
TTGTCTTTCTTATGATATTT+TGG 0.223517 8.4:+26219539 MS.gene99223:CDS
ATTTGGTTTGAGGTGTCTTT+TGG 0.287416 8.4:-26218409 None:intergenic
GATGTTTATGGTAATGATTT+TGG 0.307637 8.4:+26219414 MS.gene99223:CDS
TTACTTCGGAAATGCCTTGC+AGG 0.351265 8.4:+26219188 MS.gene99223:CDS
ACAATGGAAGGAACATAGTT+TGG 0.353074 8.4:-26218628 None:intergenic
CAAGTTACTGAGCTTGTTGA+TGG 0.358436 8.4:+26218718 MS.gene99223:CDS
TTGTCGATGGTAAGTCGTTT+TGG 0.363142 8.4:+26218773 MS.gene99223:CDS
TTACTTGCTGAAGGAGCGTT+TGG 0.366525 8.4:+26219240 MS.gene99223:CDS
TTCATCCTTTGTAACAATCT+AGG 0.367161 8.4:+26218550 MS.gene99223:CDS
CAATACATGATTTCTTCTTC+CGG 0.374442 8.4:-26219117 None:intergenic
GCTTAGTAGTGTTAAGGCTT+TGG 0.398296 8.4:+26219368 MS.gene99223:CDS
TGTTTAGTCATTGATGTTAG+AGG 0.399942 8.4:+26219138 MS.gene99223:CDS
CAAGTTGAAATGAACCAAAA+AGG 0.407254 8.4:+26218361 None:intergenic
AGGTAGGCTTAGTAGTGTTA+AGG 0.422729 8.4:+26219362 MS.gene99223:CDS
TTTGGCATTTCATCAATTCA+TGG 0.423295 8.4:+26218791 MS.gene99223:CDS
TAAAAGATATGTATAAATTG+AGG 0.446283 8.4:-26219579 None:intergenic
GGCATTTCCGAAGTAATTCT+CGG 0.456049 8.4:-26219181 None:intergenic
AGCCTGTTGCTGTTAGAAGC+GGG 0.459507 8.4:+26219445 MS.gene99223:CDS
GAGGTGTTGGATTTGGTTTG+AGG 0.469311 8.4:-26218419 None:intergenic
AAAAGATATGTATAAATTGA+GGG 0.476845 8.4:-26219578 None:intergenic
ATCAATCATGTTGTTGTCGA+TGG 0.491424 8.4:+26218760 MS.gene99223:CDS
CTTTGGCAAGCTGTGATTCG+TGG 0.491749 8.4:+26219081 MS.gene99223:CDS
TTAAGTGTTGGAAGCTTCGA+TGG 0.495846 8.4:-26218841 None:intergenic
AGCTTCCAACACTTAATCGT+TGG 0.496175 8.4:+26218848 MS.gene99223:CDS
GTGAAATGGAAAATCCTCTC+AGG 0.496490 8.4:-26218970 None:intergenic
GGAACGATAACTATGTTTAG+TGG 0.508616 8.4:+26219486 MS.gene99223:CDS
GAACGATAACTATGTTTAGT+GGG 0.510514 8.4:+26219487 MS.gene99223:CDS
AGCCTACCTTGAAACAACCT+TGG 0.510659 8.4:-26219348 None:intergenic
CCGAAGTAATTCTCGGGCAA+AGG 0.515823 8.4:-26219174 None:intergenic
ATGTTTAGTGGGGCAGAAGA+AGG 0.527980 8.4:+26219498 MS.gene99223:CDS
GGAAATGCCTTGCAGGTTGG+TGG 0.536401 8.4:+26219195 MS.gene99223:CDS
ATGAGACGACCGGTGAGGGG+TGG 0.536608 8.4:-26218475 None:intergenic
TGAGGAGAAACTATGAAAGT+TGG 0.536823 8.4:+26219316 MS.gene99223:CDS
GCATTTCCGAAGTAATTCTC+GGG 0.542365 8.4:-26219180 None:intergenic
AAGTAATTCTCGGGCAAAGG+TGG 0.543022 8.4:-26219171 None:intergenic
GAGGAGAAACTATGAAAGTT+GGG 0.544119 8.4:+26219317 MS.gene99223:CDS
AATGATGAGACGACCGGTGA+GGG 0.544298 8.4:-26218479 None:intergenic
CCTTTGCCCGAGAATTACTT+CGG 0.547729 8.4:+26219174 MS.gene99223:CDS
ATCCAAGGTTGTTTCAAGGT+AGG 0.548103 8.4:+26219346 MS.gene99223:CDS
TTCGGAAATGCCTTGCAGGT+TGG 0.554030 8.4:+26219192 MS.gene99223:CDS
TGAGACGACCGGTGAGGGGT+GGG 0.555527 8.4:-26218474 None:intergenic
GCCTGTTGCTGTTAGAAGCG+GGG 0.558922 8.4:+26219446 MS.gene99223:CDS
GAAAACAAGAGCTGATGAAA+GGG 0.562689 8.4:-26218449 None:intergenic
AGAAAACAAGAGCTGATGAA+AGG 0.564317 8.4:-26218450 None:intergenic
AAAATTTCCATTCACAAAGG+AGG 0.568603 8.4:+26218900 MS.gene99223:CDS
GTAACTTGAACTGCAAGCAA+TGG 0.569233 8.4:-26218703 None:intergenic
AAGCCTGTTGCTGTTAGAAG+CGG 0.572484 8.4:+26219444 MS.gene99223:CDS
TATAAAATTTCCATTCACAA+AGG 0.573451 8.4:+26218897 MS.gene99223:CDS
AGAAACCAACGATTAAGTGT+TGG 0.580659 8.4:-26218853 None:intergenic
CCTGTTGCTGTTAGAAGCGG+GGG 0.582350 8.4:+26219447 MS.gene99223:CDS
TTACCATAAACATCAAACCT+TGG 0.582755 8.4:-26219405 None:intergenic
GCAAGGGAATTACTTGCTGA+AGG 0.591175 8.4:+26219231 MS.gene99223:CDS
AAATGGAAAATCCTCTCAGG+TGG 0.591822 8.4:-26218967 None:intergenic
TCGTGGTCAGAATATCGATC+CGG 0.596096 8.4:+26219098 MS.gene99223:CDS
TGGCAACTAGTAGTTCTCCA+AGG 0.596495 8.4:+26219388 MS.gene99223:CDS
GTGGTGTGAAAGTGAAAGCA+AGG 0.599974 8.4:+26219214 MS.gene99223:CDS
CACGAATCACAGCTTGCCAA+AGG 0.614383 8.4:-26219080 None:intergenic
ACGCACCTAGATTGTTACAA+AGG 0.626814 8.4:-26218555 None:intergenic
ATGTTCAATGATGAGACGAC+CGG 0.630993 8.4:-26218485 None:intergenic
GTTACAAAGGATGAAACATG+AGG 0.636940 8.4:-26218542 None:intergenic
GAAATCAAAAGCTAACGAAG+AGG 0.643741 8.4:+26219014 MS.gene99223:CDS
TTTCACACCACCAACCTGCA+AGG 0.645939 8.4:-26219202 None:intergenic
GAACATCAAGAAGAAGAAGA+CGG 0.649390 8.4:+26218502 MS.gene99223:CDS
ATGATGAGACGACCGGTGAG+GGG 0.660103 8.4:-26218478 None:intergenic
CAATGATGAGACGACCGGTG+AGG 0.661803 8.4:-26218480 None:intergenic
TCATGGTCTGAAATCTCACG+TGG 0.682326 8.4:+26218808 MS.gene99223:CDS
AACGATAACTATGTTTAGTG+GGG 0.682828 8.4:+26219488 MS.gene99223:CDS
AAAACTCTATTCCACCTGAG+AGG 0.686471 8.4:+26218956 MS.gene99223:CDS
TGGTGTGAAAGTGAAAGCAA+GGG 0.721745 8.4:+26219215 MS.gene99223:CDS
CACATTGTGATGATACTGTG+AGG 0.773763 8.4:+26219298 MS.gene99223:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTTTTTTACTTTATTAAA+CGG - chr8.4:26218667-26218686 None:intergenic 10.0%
!! AAAAGATATGTATAAATTGA+GGG - chr8.4:26219581-26219600 None:intergenic 15.0%
!! TAAAAGATATGTATAAATTG+AGG - chr8.4:26219582-26219601 None:intergenic 15.0%
!! AATAAAGTAAAAAACTACGA+AGG + chr8.4:26218670-26218689 MS.gene99223:CDS 20.0%
!! TATAAAATTTCCATTCACAA+AGG + chr8.4:26218897-26218916 MS.gene99223:CDS 20.0%
!! TTATTAAACGGAAAAAAAGA+GGG - chr8.4:26218655-26218674 None:intergenic 20.0%
!! TTTATTAAACGGAAAAAAAG+AGG - chr8.4:26218656-26218675 None:intergenic 20.0%
!!! AGAAGATATTTTGTTTGTAT+TGG - chr8.4:26219040-26219059 None:intergenic 20.0%
!!! TTGTCTTTCTTATGATATTT+TGG + chr8.4:26219539-26219558 MS.gene99223:CDS 20.0%
!!! TTTGTGAATGGAAATTTTAT+AGG - chr8.4:26218898-26218917 None:intergenic 20.0%
!! TAATTTTCTCCTTTGTGAAA+TGG - chr8.4:26218987-26219006 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr8.4:26219414-26219433 MS.gene99223:CDS 25.0%
!!! TCTTTCTTATGATATTTTGG+AGG + chr8.4:26219542-26219561 MS.gene99223:CDS 25.0%
!!! TTATGGTAATGATTTTGGTT+GGG + chr8.4:26219419-26219438 MS.gene99223:CDS 25.0%
!!! TTTATGGTAATGATTTTGGT+TGG + chr8.4:26219418-26219437 MS.gene99223:CDS 25.0%
AAAATTTCCATTCACAAAGG+AGG + chr8.4:26218900-26218919 MS.gene99223:CDS 30.0%
AACGATAACTATGTTTAGTG+GGG + chr8.4:26219488-26219507 MS.gene99223:CDS 30.0%
CAATACATGATTTCTTCTTC+CGG - chr8.4:26219120-26219139 None:intergenic 30.0%
GAACGATAACTATGTTTAGT+GGG + chr8.4:26219487-26219506 MS.gene99223:CDS 30.0%
TGTTTAGTCATTGATGTTAG+AGG + chr8.4:26219138-26219157 MS.gene99223:CDS 30.0%
TTACCATAAACATCAAACCT+TGG - chr8.4:26219408-26219427 None:intergenic 30.0%
TTCATCCTTTGTAACAATCT+AGG + chr8.4:26218550-26218569 MS.gene99223:CDS 30.0%
TTTGGCATTTCATCAATTCA+TGG + chr8.4:26218791-26218810 MS.gene99223:CDS 30.0%
! AAAAATCCAAGGTTGTTTCA+AGG + chr8.4:26219342-26219361 MS.gene99223:CDS 30.0%
! TATGAAAGAGAAGTCCTTTT+TGG - chr8.4:26218378-26218397 None:intergenic 30.0%
! TTATTTTCCTCCTTTGTGAA+TGG - chr8.4:26218910-26218929 None:intergenic 30.0%
!! TATGATATTTTGGAGGCTAT+GGG + chr8.4:26219549-26219568 MS.gene99223:CDS 30.0%
!! TTATGATATTTTGGAGGCTA+TGG + chr8.4:26219548-26219567 MS.gene99223:CDS 30.0%
!!! TATGGTAATGATTTTGGTTG+GGG + chr8.4:26219420-26219439 MS.gene99223:CDS 30.0%
AAAAAAGAGGGAACAATGGA+AGG - chr8.4:26218643-26218662 None:intergenic 35.0%
AAAGTTGGGTGAAAAATCCA+AGG + chr8.4:26219331-26219350 MS.gene99223:CDS 35.0%
ACAATGGAAGGAACATAGTT+TGG - chr8.4:26218631-26218650 None:intergenic 35.0%
AGAAAACAAGAGCTGATGAA+AGG - chr8.4:26218453-26218472 None:intergenic 35.0%
AGAAACCAACGATTAAGTGT+TGG - chr8.4:26218856-26218875 None:intergenic 35.0%
ATCAATCATGTTGTTGTCGA+TGG + chr8.4:26218760-26218779 MS.gene99223:CDS 35.0%
GAAAACAAGAGCTGATGAAA+GGG - chr8.4:26218452-26218471 None:intergenic 35.0%
GAAATCAAAAGCTAACGAAG+AGG + chr8.4:26219014-26219033 MS.gene99223:CDS 35.0%
GAACATCAAGAAGAAGAAGA+CGG + chr8.4:26218502-26218521 MS.gene99223:CDS 35.0%
GAGGAGAAACTATGAAAGTT+GGG + chr8.4:26219317-26219336 MS.gene99223:CDS 35.0%
GGAACGATAACTATGTTTAG+TGG + chr8.4:26219486-26219505 MS.gene99223:CDS 35.0%
GTTACAAAGGATGAAACATG+AGG - chr8.4:26218545-26218564 None:intergenic 35.0%
TCTCCAAGGTTTGATGTTTA+TGG + chr8.4:26219402-26219421 MS.gene99223:CDS 35.0%
TGAGGAGAAACTATGAAAGT+TGG + chr8.4:26219316-26219335 MS.gene99223:CDS 35.0%
! GAGGATTTTCCATTTCACAA+AGG + chr8.4:26218975-26218994 MS.gene99223:CDS 35.0%
! TTGTGTTTTCAGCTATTGCA+TGG - chr8.4:26218588-26218607 None:intergenic 35.0%
!! AAGCACTTTTAACTCACCTT+TGG + chr8.4:26219064-26219083 MS.gene99223:CDS 35.0%
!!! ATTTGGTTTGAGGTGTCTTT+TGG - chr8.4:26218412-26218431 None:intergenic 35.0%
!!! CTTGTTGATGGCATTTTCAT+TGG + chr8.4:26218730-26218749 MS.gene99223:CDS 35.0%
!!! TGTTTTTGAGGTGTTGGATT+TGG - chr8.4:26218429-26218448 None:intergenic 35.0%
!!! TTTGGTTTGAGGTGTCTTTT+GGG - chr8.4:26218411-26218430 None:intergenic 35.0%
AAAACTCTATTCCACCTGAG+AGG + chr8.4:26218956-26218975 MS.gene99223:CDS 40.0%
AAATGGAAAATCCTCTCAGG+TGG - chr8.4:26218970-26218989 None:intergenic 40.0%
ACGCACCTAGATTGTTACAA+AGG - chr8.4:26218558-26218577 None:intergenic 40.0%
AGCTTCCAACACTTAATCGT+TGG + chr8.4:26218848-26218867 MS.gene99223:CDS 40.0%
AGGTAGGCTTAGTAGTGTTA+AGG + chr8.4:26219362-26219381 MS.gene99223:CDS 40.0%
ATGTTCAATGATGAGACGAC+CGG - chr8.4:26218488-26218507 None:intergenic 40.0%
CACATTGTGATGATACTGTG+AGG + chr8.4:26219298-26219317 MS.gene99223:CDS 40.0%
CGGAAAAAAAGAGGGAACAA+TGG - chr8.4:26218647-26218666 None:intergenic 40.0%
GCATTTCCGAAGTAATTCTC+GGG - chr8.4:26219183-26219202 None:intergenic 40.0%
GCTTAGTAGTGTTAAGGCTT+TGG + chr8.4:26219368-26219387 MS.gene99223:CDS 40.0%
GGCATTTCCGAAGTAATTCT+CGG - chr8.4:26219184-26219203 None:intergenic 40.0%
GTAACTTGAACTGCAAGCAA+TGG - chr8.4:26218706-26218725 None:intergenic 40.0%
GTGAAATGGAAAATCCTCTC+AGG - chr8.4:26218973-26218992 None:intergenic 40.0%
TGGTGTGAAAGTGAAAGCAA+GGG + chr8.4:26219215-26219234 MS.gene99223:CDS 40.0%
! ATCCAAGGTTGTTTCAAGGT+AGG + chr8.4:26219346-26219365 MS.gene99223:CDS 40.0%
! TTGTCGATGGTAAGTCGTTT+TGG + chr8.4:26218773-26218792 MS.gene99223:CDS 40.0%
!! CAAGTTACTGAGCTTGTTGA+TGG + chr8.4:26218718-26218737 MS.gene99223:CDS 40.0%
!! TTAAGTGTTGGAAGCTTCGA+TGG - chr8.4:26218844-26218863 None:intergenic 40.0%
!!! AAAGGGTGTTTTTGAGGTGT+TGG - chr8.4:26218435-26218454 None:intergenic 40.0%
!!! CTGATGAAAGGGTGTTTTTG+AGG - chr8.4:26218441-26218460 None:intergenic 40.0%
!!! GAGGTGTCTTTTGGGTTTTT+GGG - chr8.4:26218403-26218422 None:intergenic 40.0%
!!! TGAGGTGTCTTTTGGGTTTT+TGG - chr8.4:26218404-26218423 None:intergenic 40.0%
AAGTAATTCTCGGGCAAAGG+TGG - chr8.4:26219174-26219193 None:intergenic 45.0%
AGCCTACCTTGAAACAACCT+TGG - chr8.4:26219351-26219370 None:intergenic 45.0%
ATGTTTAGTGGGGCAGAAGA+AGG + chr8.4:26219498-26219517 MS.gene99223:CDS 45.0%
CCTTTGCCCGAGAATTACTT+CGG + chr8.4:26219174-26219193 MS.gene99223:CDS 45.0%
GTGGTGTGAAAGTGAAAGCA+AGG + chr8.4:26219214-26219233 MS.gene99223:CDS 45.0%
TCGTGGTCAGAATATCGATC+CGG + chr8.4:26219098-26219117 MS.gene99223:CDS 45.0%
TGGCAACTAGTAGTTCTCCA+AGG + chr8.4:26219388-26219407 MS.gene99223:CDS 45.0%
TTACTTCGGAAATGCCTTGC+AGG + chr8.4:26219188-26219207 MS.gene99223:CDS 45.0%
! AAGCCTGTTGCTGTTAGAAG+CGG + chr8.4:26219444-26219463 MS.gene99223:CDS 45.0%
! GCAAGGGAATTACTTGCTGA+AGG + chr8.4:26219231-26219250 MS.gene99223:CDS 45.0%
! GGGGGTGCAAATAAAAGTGA+TGG + chr8.4:26219465-26219484 MS.gene99223:CDS 45.0%
! TCATGGTCTGAAATCTCACG+TGG + chr8.4:26218808-26218827 MS.gene99223:CDS 45.0%
!! GAGGTGTTGGATTTGGTTTG+AGG - chr8.4:26218422-26218441 None:intergenic 45.0%
!! TTACTTGCTGAAGGAGCGTT+TGG + chr8.4:26219240-26219259 MS.gene99223:CDS 45.0%
AATGATGAGACGACCGGTGA+GGG - chr8.4:26218482-26218501 None:intergenic 50.0%
CACGAATCACAGCTTGCCAA+AGG - chr8.4:26219083-26219102 None:intergenic 50.0%
CCGAAGTAATTCTCGGGCAA+AGG - chr8.4:26219177-26219196 None:intergenic 50.0%
CTTTGGCAAGCTGTGATTCG+TGG + chr8.4:26219081-26219100 MS.gene99223:CDS 50.0%
TTCGGAAATGCCTTGCAGGT+TGG + chr8.4:26219192-26219211 MS.gene99223:CDS 50.0%
TTTCACACCACCAACCTGCA+AGG - chr8.4:26219205-26219224 None:intergenic 50.0%
! AGCCTGTTGCTGTTAGAAGC+GGG + chr8.4:26219445-26219464 MS.gene99223:CDS 50.0%
ATGATGAGACGACCGGTGAG+GGG - chr8.4:26218481-26218500 None:intergenic 55.0%
CAATGATGAGACGACCGGTG+AGG - chr8.4:26218483-26218502 None:intergenic 55.0%
! CCTGTTGCTGTTAGAAGCGG+GGG + chr8.4:26219447-26219466 MS.gene99223:CDS 55.0%
! GCCTGTTGCTGTTAGAAGCG+GGG + chr8.4:26219446-26219465 MS.gene99223:CDS 55.0%
! GGAAATGCCTTGCAGGTTGG+TGG + chr8.4:26219195-26219214 MS.gene99223:CDS 55.0%
! GTTTTCTTCCCACCCCTCAC+CGG + chr8.4:26218466-26218485 MS.gene99223:CDS 55.0%
CCCCCGCTTCTAACAGCAAC+AGG - chr8.4:26219450-26219469 None:intergenic 60.0%
ATGAGACGACCGGTGAGGGG+TGG - chr8.4:26218478-26218497 None:intergenic 65.0%
TGAGACGACCGGTGAGGGGT+GGG - chr8.4:26218477-26218496 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 26218370 26219602 26218370 ID=MS.gene99223
chr8.4 mRNA 26218370 26219602 26218370 ID=MS.gene99223.t1;Parent=MS.gene99223
chr8.4 exon 26218370 26219602 26218370 ID=MS.gene99223.t1.exon1;Parent=MS.gene99223.t1
chr8.4 CDS 26218370 26219602 26218370 ID=cds.MS.gene99223.t1;Parent=MS.gene99223.t1
Gene Sequence

>MS.gene99223

ATGAACCAAAAAGGACTTCTCTTTCATAATCCCAAAAACCCAAAAGACACCTCAAACCAAATCCAACACCTCAAAAACACCCTTTCATCAGCTCTTGTTTTCTTCCCACCCCTCACCGGTCGTCTCATCATTGAACATCAAGAAGAAGAAGACGGCGACAACAACAACACAACCTCATGTTTCATCCTTTGTAACAATCTAGGTGCGTTATTTATCCATGCAATAGCTGAAAACACAAGTGTCTCTGATATTCTTAAACCAAACTATGTTCCTTCCATTGTTCCCTCTTTTTTTCCGTTTAATAAAGTAAAAAACTACGAAGGCACATCAAAACCATTGCTTGCAGTTCAAGTTACTGAGCTTGTTGATGGCATTTTCATTGGCTTTTCAATCAATCATGTTGTTGTCGATGGTAAGTCGTTTTGGCATTTCATCAATTCATGGTCTGAAATCTCACGTGGTTTTGATCGACCATCGAAGCTTCCAACACTTAATCGTTGGTTTCTCGATGACTCAATTGAAATTCCTATAAAATTTCCATTCACAAAGGAGGAAAATAAAGAAAGTACTGAAGCTGAAACTGATGAAAACTCTATTCCACCTGAGAGGATTTTCCATTTCACAAAGGAGAAAATTACGAAACTGAAATCAAAAGCTAACGAAGAGGCCAATACAAACAAAATATCTTCTTTACAAGCACTTTTAACTCACCTTTGGCAAGCTGTGATTCGTGGTCAGAATATCGATCCGGAAGAAGAAATCATGTATTGTTTAGTCATTGATGTTAGAGGCAGAATTATTCCACCTTTGCCCGAGAATTACTTCGGAAATGCCTTGCAGGTTGGTGGTGTGAAAGTGAAAGCAAGGGAATTACTTGCTGAAGGAGCGTTTGGAAAAGTTGCTTTTGAGATGAATAAGATGATAGCTTCACATTGTGATGATACTGTGAGGAGAAACTATGAAAGTTGGGTGAAAAATCCAAGGTTGTTTCAAGGTAGGCTTAGTAGTGTTAAGGCTTTGGCAACTAGTAGTTCTCCAAGGTTTGATGTTTATGGTAATGATTTTGGTTGGGGTAAGCCTGTTGCTGTTAGAAGCGGGGGTGCAAATAAAAGTGATGGAACGATAACTATGTTTAGTGGGGCAGAAGAAGGTAGTATTGATGTTGAAGTTTGTCTTTCTTATGATATTTTGGAGGCTATGGGAAATGACCCTCAATTTATACATATCTTTTAA
Protein sequence

>MS.gene99223.t1

MNQKGLLFHNPKNPKDTSNQIQHLKNTLSSALVFFPPLTGRLIIEHQEEEDGDNNNTTSCFILCNNLGALFIHAIAENTSVSDILKPNYVPSIVPSFFPFNKVKNYEGTSKPLLAVQVTELVDGIFIGFSINHVVVDGKSFWHFINSWSEISRGFDRPSKLPTLNRWFLDDSIEIPIKFPFTKEENKESTEAETDENSIPPERIFHFTKEKITKLKSKANEEANTNKISSLQALLTHLWQAVIRGQNIDPEEEIMYCLVIDVRGRIIPPLPENYFGNALQVGGVKVKARELLAEGAFGKVAFEMNKMIASHCDDTVRRNYESWVKNPRLFQGRLSSVKALATSSSPRFDVYGNDFGWGKPVAVRSGGANKSDGTITMFSGAEEGSIDVEVCLSYDILEAMGNDPQFIHIF