AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene03202

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03202.t1 MTR_3g088420 94.457 433 24 0 1 433 1 433 0.0 858
MS.gene03202.t1 MTR_3g026310 72.098 448 110 3 1 433 1 448 0.0 657
MS.gene03202.t1 MTR_4g022670 67.574 441 135 2 1 433 1 441 0.0 614
MS.gene03202.t1 MTR_4g022800 68.552 442 130 3 1 433 1 442 0.0 612
MS.gene03202.t1 MTR_4g022825 67.273 440 137 1 1 433 1 440 0.0 603
MS.gene03202.t1 MTR_4g022720 68.046 435 137 2 1 433 1 435 0.0 600
MS.gene03202.t1 MTR_7g026860 66.893 441 135 3 1 433 1 438 0.0 588
MS.gene03202.t1 MTR_8g041770 61.712 444 156 6 1 433 1 441 0.0 535
MS.gene03202.t1 MTR_0190s0020 60.811 444 161 6 1 433 1 442 0.0 528
MS.gene03202.t1 MTR_4g022760 60.317 441 123 3 1 433 1 397 0.0 517
MS.gene03202.t1 MTR_4g094842 56.347 449 175 8 1 431 1 446 1.93e-167 478
MS.gene03202.t1 MTR_4g094835 54.241 448 180 10 1 429 1 442 3.04e-163 467
MS.gene03202.t1 MTR_4g094828 51.648 455 195 11 1 434 1 451 4.13e-152 439
MS.gene03202.t1 MTR_6g016815 54.965 433 129 7 1 433 15 381 8.44e-152 436
MS.gene03202.t1 MTR_8g079710 54.139 447 181 12 1 429 1 441 5.68e-149 431
MS.gene03202.t1 MTR_4g094838 51.448 449 174 9 4 429 1 428 1.43e-147 427
MS.gene03202.t1 MTR_4g022290 51.441 451 189 11 1 430 1 442 2.34e-146 424
MS.gene03202.t1 MTR_4g094832 54.787 376 149 7 69 429 57 426 9.89e-135 394
MS.gene03202.t1 MTR_3g101900 39.352 432 239 10 19 434 25 449 3.51e-94 291
MS.gene03202.t1 MTR_8g040780 63.677 223 77 2 210 428 5 227 1.70e-93 282
MS.gene03202.t1 MTR_8g040760 65.566 212 67 2 1 206 1 212 4.38e-91 275
MS.gene03202.t1 MTR_3g101930 38.228 429 241 10 24 432 30 454 1.28e-86 271
MS.gene03202.t1 MTR_3g102020 37.701 435 246 10 19 434 24 452 1.25e-85 269
MS.gene03202.t1 MTR_3g101970 36.961 441 240 11 11 434 19 438 2.81e-83 262
MS.gene03202.t1 MTR_3g102010 37.762 429 243 10 24 434 30 452 7.66e-83 262
MS.gene03202.t1 MTR_3g101960 36.343 432 243 11 19 428 24 445 1.68e-79 253
MS.gene03202.t1 MTR_7g026850 72.903 155 42 0 29 183 12 166 5.88e-79 242
MS.gene03202.t1 MTR_3g070170 31.590 478 264 13 2 433 10 470 7.44e-63 210
MS.gene03202.t1 MTR_4g015180 32.704 477 246 15 20 433 23 487 2.69e-57 196
MS.gene03202.t1 MTR_6g034485 71.774 124 34 1 189 312 3 125 3.47e-57 184
MS.gene03202.t1 MTR_5g081960 30.672 476 255 11 19 429 17 482 9.05e-57 194
MS.gene03202.t1 MTR_7g026770 49.362 235 77 4 109 322 80 293 9.91e-57 189
MS.gene03202.t1 MTR_4g094825 56.287 167 66 4 16 176 83 248 5.62e-53 182
MS.gene03202.t1 MTR_4g094825 51.656 151 60 2 285 433 252 391 9.14e-42 152
MS.gene03202.t1 MTR_6g045553 29.741 464 282 12 4 429 14 471 7.76e-49 173
MS.gene03202.t1 MTR_6g034495 62.774 137 41 3 49 180 33 164 1.01e-47 161
MS.gene03202.t1 MTR_3g102030 33.225 307 171 6 87 389 44 320 1.59e-45 160
MS.gene03202.t1 MTR_7g026830 75.000 84 21 0 82 165 14 97 1.83e-39 137
MS.gene03202.t1 MTR_3g026950 27.869 366 220 12 52 385 51 404 1.43e-30 122
MS.gene03202.t1 MTR_8g096530 28.019 414 229 15 53 426 57 441 1.65e-30 122
MS.gene03202.t1 MTR_7g026800 44.375 160 58 3 215 372 30 160 2.36e-29 113
MS.gene03202.t1 MTR_4g007540 24.490 441 284 15 4 417 3 421 7.76e-29 117
MS.gene03202.t1 MTR_2g105330 25.594 379 248 12 55 415 56 418 1.90e-28 116
MS.gene03202.t1 MTR_8g075610 26.684 386 246 15 55 422 47 413 3.38e-28 115
MS.gene03202.t1 MTR_4g022780 42.945 163 62 3 214 374 30 163 4.37e-28 109
MS.gene03202.t1 MTR_2g064495 25.654 382 244 13 55 415 56 418 1.63e-27 114
MS.gene03202.t1 MTR_3g098980 26.781 351 210 11 55 385 66 389 3.32e-27 113
MS.gene03202.t1 MTR_7g026780 50.000 110 31 2 324 433 26 111 2.35e-26 102
MS.gene03202.t1 MTR_3g049150 28.947 380 221 17 42 384 32 399 3.22e-26 110
MS.gene03202.t1 MTR_4g013050 25.419 358 205 9 49 385 47 363 3.95e-24 103
MS.gene03202.t1 MTR_4g097090 25.223 448 282 19 21 432 27 457 1.44e-22 100
MS.gene03202.t1 MTR_4g127650 25.604 414 263 15 49 432 59 457 1.52e-22 99.8
MS.gene03202.t1 MTR_7g085930 26.166 386 218 13 52 390 56 421 6.15e-20 92.0
MS.gene03202.t1 MTR_2g089670 26.847 406 237 15 21 384 42 429 1.70e-19 90.9
MS.gene03202.t1 MTR_1g081660 25.515 388 241 15 24 385 31 396 1.92e-19 90.5
MS.gene03202.t1 MTR_7g014330 24.096 415 248 15 19 388 21 413 2.34e-19 90.5
MS.gene03202.t1 MTR_6g015830 23.928 443 287 13 15 422 25 452 1.79e-18 87.8
MS.gene03202.t1 MTR_1g040195 27.014 422 257 19 25 427 23 412 2.40e-18 87.0
MS.gene03202.t1 MTR_7g026870 48.718 78 40 0 266 343 74 151 5.37e-18 80.9
MS.gene03202.t1 MTR_6g015850 23.714 447 288 13 8 421 22 448 5.48e-18 86.3
MS.gene03202.t1 MTR_7g027175 24.365 394 267 12 50 431 63 437 9.47e-18 85.5
MS.gene03202.t1 MTR_2g089755 25.173 433 262 19 24 409 37 454 1.01e-17 85.5
MS.gene03202.t1 MTR_6g015320 24.552 391 258 11 24 387 29 409 4.85e-17 83.2
MS.gene03202.t1 MTR_7g026880 63.462 52 19 0 382 433 2 53 6.60e-17 74.7
MS.gene03202.t1 MTR_5g076700 23.303 442 278 17 25 429 24 441 6.99e-17 82.8
MS.gene03202.t1 MTR_7g085990 26.471 306 199 8 52 337 52 351 8.82e-17 82.8
MS.gene03202.t1 MTR_3g087640 25.282 443 278 16 22 420 29 462 4.96e-16 80.5
MS.gene03202.t1 MTR_7g114550 21.847 444 281 19 25 429 24 440 1.23e-15 79.0
MS.gene03202.t1 MTR_2g089650 23.944 426 244 16 38 409 43 442 2.04e-15 78.6
MS.gene03202.t1 MTR_7g014200 24.146 410 255 14 22 388 28 424 2.61e-15 78.2
MS.gene03202.t1 MTR_7g014620 25.175 429 254 17 24 414 15 414 3.16e-15 77.8
MS.gene03202.t1 MTR_3g028210 22.511 462 288 17 4 415 8 449 4.08e-15 77.4
MS.gene03202.t1 MTR_3g086020 25.895 363 222 11 55 406 61 387 5.39e-15 77.0
MS.gene03202.t1 MTR_7g086000 30.385 260 159 12 58 302 63 315 6.45e-15 77.0
MS.gene03202.t1 MTR_8g012750 24.816 407 254 12 22 384 24 422 8.21e-15 76.6
MS.gene03202.t1 MTR_7g114470 22.955 440 283 16 25 429 25 443 9.74e-15 76.3
MS.gene03202.t1 MTR_1g040175 27.305 282 175 11 50 317 59 324 1.45e-14 75.9
MS.gene03202.t1 MTR_7g015860 25.249 301 172 10 122 385 129 413 3.61e-14 74.7
MS.gene03202.t1 MTR_4g055170 25.413 303 196 10 55 343 69 355 1.30e-13 72.8
MS.gene03202.t1 MTR_7g084910 28.102 274 174 9 49 308 62 326 1.43e-13 72.8
MS.gene03202.t1 MTR_0502s0010 28.879 232 149 9 95 317 1 225 1.78e-13 70.1
MS.gene03202.t1 MTR_7g114520 24.365 394 239 19 25 385 25 392 2.02e-13 72.4
MS.gene03202.t1 MTR_1g053315 25.065 387 248 19 55 416 78 447 2.03e-13 72.4
MS.gene03202.t1 MTR_7g084940 27.875 287 173 11 49 316 61 332 6.36e-13 70.5
MS.gene03202.t1 MTR_3g465980 25.589 297 188 9 55 336 68 346 6.54e-13 70.5
MS.gene03202.t1 MTR_3g112180 24.297 391 252 16 52 420 58 426 1.02e-12 70.1
MS.gene03202.t1 MTR_3g049140 26.407 231 146 9 204 414 6 232 1.04e-12 68.2
MS.gene03202.t1 MTR_5g090810 24.372 398 236 17 32 385 36 412 1.68e-12 69.3
MS.gene03202.t1 MTR_5g090880 23.971 413 245 17 55 422 67 455 1.81e-12 69.3
MS.gene03202.t1 MTR_5g090830 24.181 397 238 16 32 385 36 412 5.16e-12 67.8
MS.gene03202.t1 MTR_7g014360 25.856 263 174 10 138 388 90 343 1.74e-11 65.9
MS.gene03202.t1 MTR_3g465990 25.725 276 179 10 55 316 68 331 2.52e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03202.t1 AT5G67160 40.411 438 236 11 6 434 12 433 1.45e-98 301
MS.gene03202.t1 AT3G50300 42.056 428 230 11 1 414 1 424 7.58e-97 298
MS.gene03202.t1 AT5G67150 38.546 454 252 11 1 434 1 447 3.21e-96 296
MS.gene03202.t1 AT3G50280 38.427 445 254 11 1 430 1 440 6.78e-93 287
MS.gene03202.t1 AT3G50270 39.450 436 248 11 12 434 17 449 2.75e-91 283
MS.gene03202.t1 AT5G38130 40.465 430 232 12 1 414 21 442 1.39e-88 277
MS.gene03202.t1 AT5G42830 37.097 434 255 10 15 434 21 450 4.49e-86 270
MS.gene03202.t1 AT5G07860 36.807 451 257 14 2 432 6 448 4.43e-78 249
MS.gene03202.t1 AT5G07870 36.364 462 258 14 2 433 6 461 9.00e-77 246
MS.gene03202.t1 AT5G07850 35.153 458 261 15 2 433 6 453 2.36e-70 229
MS.gene03202.t1 AT2G39980 32.712 483 254 13 11 433 9 480 7.20e-66 218
MS.gene03202.t1 AT3G50280 39.103 312 178 6 127 430 3 310 1.17e-65 213
MS.gene03202.t1 AT3G50290 42.963 270 141 5 122 382 4 269 1.27e-61 201
MS.gene03202.t1 AT5G01210 31.618 408 228 10 66 433 73 469 3.34e-57 196
MS.gene03202.t1 AT5G23940 31.429 455 274 15 4 429 3 448 5.21e-55 190
MS.gene03202.t1 AT5G48930 26.873 387 236 17 53 414 54 418 3.47e-27 113
MS.gene03202.t1 AT3G48720 27.616 344 217 11 55 385 61 385 3.45e-26 110
MS.gene03202.t1 AT5G41040 26.225 347 226 10 52 385 66 395 2.86e-25 107
MS.gene03202.t1 AT5G41040 26.801 347 224 11 52 385 82 411 3.50e-25 107
MS.gene03202.t1 AT5G57840 28.715 397 227 19 49 414 51 422 1.68e-22 99.8
MS.gene03202.t1 AT2G19070 26.014 419 253 19 50 431 53 451 8.41e-22 97.8
MS.gene03202.t1 AT3G29635 26.190 420 271 14 24 414 29 438 4.29e-21 95.5
MS.gene03202.t1 AT3G29590 24.276 449 302 12 2 424 6 442 6.63e-21 95.1
MS.gene03202.t1 AT3G26040 26.290 407 246 17 51 434 55 430 1.11e-20 94.4
MS.gene03202.t1 AT5G63560 25.215 349 215 12 55 385 61 381 3.49e-19 89.7
MS.gene03202.t1 AT1G28680 24.390 451 285 18 1 427 1 419 5.99e-19 89.4
MS.gene03202.t1 AT3G29670 22.706 436 285 15 20 422 25 441 2.44e-18 87.4
MS.gene03202.t1 AT2G40230 27.136 398 243 17 11 389 22 391 1.23e-17 85.1
MS.gene03202.t1 AT1G24420 23.725 451 299 16 4 434 5 430 6.66e-17 82.8
MS.gene03202.t1 AT1G24420 23.725 451 299 16 4 434 5 430 6.66e-17 82.8
MS.gene03202.t1 AT1G28680 24.932 365 230 16 1 343 1 343 2.71e-16 80.9
MS.gene03202.t1 AT3G30280 22.716 405 272 16 49 434 55 437 2.45e-15 78.2
MS.gene03202.t1 AT1G24430 24.934 381 214 17 54 406 61 397 6.70e-15 76.6
MS.gene03202.t1 AT5G39090 28.571 385 217 18 42 394 56 414 1.66e-14 75.5
MS.gene03202.t1 AT3G29680 24.112 394 249 12 24 385 25 400 1.35e-13 72.8
MS.gene03202.t1 AT5G23970 23.385 449 287 18 4 429 3 417 1.51e-13 72.4
MS.gene03202.t1 AT1G03390 23.342 407 258 16 42 415 59 444 2.80e-13 72.0
MS.gene03202.t1 AT5G61160 22.251 391 267 13 22 385 23 403 5.20e-13 70.9
MS.gene03202.t1 AT5G47950 23.429 350 218 15 50 385 56 369 1.35e-12 69.7
MS.gene03202.t1 AT5G47980 24.000 400 263 14 54 434 60 437 2.67e-12 68.9
MS.gene03202.t1 AT1G78990 25.141 354 222 15 53 385 65 396 1.40e-11 66.6

Find 90 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCCGTGGTTTGATGTTTA+TGG 0.126200 3.3:+73803054 MS.gene03202:CDS
CCATTGCCTGAAGATTATTT+TGG 0.195668 3.3:+73802823 MS.gene03202:CDS
ATTGGCTAATGTATTATTAT+TGG 0.203749 3.3:-73803025 None:intergenic
GTGTCTTCCTCACAAGATTT+TGG 0.229479 3.3:+73803185 MS.gene03202:CDS
ACTAATTGCACCCACCAAAA+AGG 0.252147 3.3:+73802045 MS.gene03202:CDS
GATGTTTATGGTAATGATTT+TGG 0.308658 3.3:+73803066 MS.gene03202:CDS
GCCTGCAAGTGGTGGGAAAA+AGG 0.338259 3.3:-73802140 None:intergenic
ACGTTCTAGTGTTATGAGTT+TGG 0.352542 3.3:-73802503 None:intergenic
TTCGTGTAGGTGAAATTGTT+TGG 0.357672 3.3:-73802752 None:intergenic
GGAATGATTACTGTATTTGC+AGG 0.362612 3.3:+73803132 MS.gene03202:CDS
TTCAGATGTTGAATTTGATT+TGG 0.376205 3.3:-73802094 None:intergenic
GTGGGCTTGGTAAAGGTGCT+TGG 0.384077 3.3:+73802902 MS.gene03202:CDS
ACACGAAGAAGTTTATTATA+TGG 0.390132 3.3:+73802768 MS.gene03202:CDS
TTTAACCACGCGGTTGTTGA+TGG 0.400033 3.3:+73802415 MS.gene03202:CDS
GCATTTCCAAAATAATCTTC+AGG 0.400923 3.3:-73802829 None:intergenic
AATGAAAGCAGGACAGTTGT+TGG 0.413215 3.3:+73802873 MS.gene03202:CDS
AATTTGGTGACTTGATTGTA+TGG 0.415203 3.3:-73801976 None:intergenic
TTGTATGGGTAGAGAGGACT+TGG 0.418563 3.3:-73801961 None:intergenic
AAACCACGGGGAATTGCTAT+TGG 0.430657 3.3:-73803043 None:intergenic
GGGAAATGACCATGACTTCA+TGG 0.444511 3.3:+73803215 MS.gene03202:CDS
AGGAAAAGAAGAAGGTAGTA+TGG 0.449506 3.3:+73803152 MS.gene03202:CDS
GAAATGCTGTTGAAGTTTGT+AGG 0.451612 3.3:+73802845 MS.gene03202:CDS
TTAGTTGACGGCATCTTCAT+TGG 0.458413 3.3:+73802385 MS.gene03202:CDS
TTGAAAGCCAACATGGAGAT+TGG 0.465372 3.3:+73802667 MS.gene03202:CDS
CGCAGTGGAAATAAAATAAG+TGG 0.469980 3.3:+73803111 MS.gene03202:CDS
TGAAGAATCACTACGAGTCT+TGG 0.474671 3.3:+73802965 MS.gene03202:CDS
CCAAAATAATCTTCAGGCAA+TGG 0.482695 3.3:-73802823 None:intergenic
TGGGCTTGGTAAAGGTGCTT+GGG 0.485107 3.3:+73802903 MS.gene03202:CDS
ATTTAACTCCATGGGATCTC+CGG 0.488953 3.3:+73802017 MS.gene03202:CDS
GAAGATGGTGGGCTTGGTAA+AGG 0.490096 3.3:+73802895 MS.gene03202:CDS
ACCTTGATCTAGCTCCTATC+GGG 0.496130 3.3:-73802792 None:intergenic
AACCTTGATCTAGCTCCTAT+CGG 0.500296 3.3:-73802793 None:intergenic
GCATGTGACGGAACATGAAA+TGG 0.505364 3.3:-73802194 None:intergenic
AATCACTACGAGTCTTGGTT+GGG 0.505413 3.3:+73802970 MS.gene03202:CDS
GTTGGTACCAATCTCCATGT+TGG 0.507485 3.3:-73802674 None:intergenic
GAATCACTACGAGTCTTGGT+TGG 0.508104 3.3:+73802969 MS.gene03202:CDS
TCGAGACGGCCTGCAAGTGG+TGG 0.521804 3.3:-73802148 None:intergenic
GCAGGACAGTTGTTGGAAGA+TGG 0.523872 3.3:+73802880 MS.gene03202:CDS
TATAGTGAAAGGAAATCGTA+TGG 0.523888 3.3:-73802551 None:intergenic
AACTCATAACACTAGAACGT+TGG 0.524522 3.3:+73802506 MS.gene03202:CDS
TACCATAAACATCAAACCAC+GGG 0.529973 3.3:-73803056 None:intergenic
TTTCCACTGCGAATTGCCAC+AGG 0.532396 3.3:-73803099 None:intergenic
GACAGTTGTTGGAAGATGGT+GGG 0.539492 3.3:+73802884 MS.gene03202:CDS
TGTGATCTGTTATTTCGAGA+CGG 0.540547 3.3:-73802162 None:intergenic
AAATGGAATAACCTCTCTGA+TGG 0.546561 3.3:-73802616 None:intergenic
ACTTGCGTAGGTGACATACT+TGG 0.546620 3.3:+73802259 MS.gene03202:CDS
TGTAGGGTTACAATGAAAGC+AGG 0.550470 3.3:+73802862 MS.gene03202:CDS
TTGTTGGAAGATGGTGGGCT+TGG 0.553654 3.3:+73802889 MS.gene03202:CDS
ACCAGCATTGTTGCATGTGA+CGG 0.553807 3.3:-73802206 None:intergenic
AAATGCTGTTGAAGTTTGTA+GGG 0.553867 3.3:+73802846 MS.gene03202:CDS
TCCCGATAGGAGCTAGATCA+AGG 0.559796 3.3:+73802791 MS.gene03202:CDS
AACGGATCACAGAGCGCCAA+AGG 0.567411 3.3:-73802729 None:intergenic
AAATAATCTTCAGGCAATGG+TGG 0.571644 3.3:-73802820 None:intergenic
GGACAGTTGTTGGAAGATGG+TGG 0.574775 3.3:+73802883 MS.gene03202:CDS
ACTGAAATTGAAAGCCAACA+TGG 0.579416 3.3:+73802660 MS.gene03202:CDS
TATAATAAACTTCTTCGTGT+AGG 0.589257 3.3:-73802765 None:intergenic
ATTTCGAGACGGCCTGCAAG+TGG 0.589823 3.3:-73802151 None:intergenic
TGGGTGCAATTAGTAGGTAC+CGG 0.591093 3.3:-73802036 None:intergenic
GTTTATTATATGGTCCCGAT+AGG 0.594388 3.3:+73802778 MS.gene03202:CDS
AAGTGTTCTCCGCTGCGGCA+TGG 0.595760 3.3:-73802240 None:intergenic
GTAGGTGAAATTGTTTGGAA+CGG 0.596109 3.3:-73802747 None:intergenic
TGAATTTGATTTGGCACTAG+AGG 0.597459 3.3:-73802085 None:intergenic
TCCGTCACATGCAACAATGC+TGG 0.599979 3.3:+73802205 MS.gene03202:CDS
ACACAACATCCATGAAGTCA+TGG 0.605152 3.3:-73803224 None:intergenic
ATCGACGATGGGAGGAACGT+AGG 0.605346 3.3:-73802287 None:intergenic
AGTAGGTACCGGAGATCCCA+TGG 0.606618 3.3:-73802025 None:intergenic
GTATTTGCAGGAAAAGAAGA+AGG 0.607179 3.3:+73803144 MS.gene03202:CDS
CGAGACGGCCTGCAAGTGGT+GGG 0.611766 3.3:-73802147 None:intergenic
ATTTGGTGACTTGATTGTAT+GGG 0.612690 3.3:-73801975 None:intergenic
ATGGGATGTTGAATACCACT+TGG 0.613611 3.3:-73802532 None:intergenic
CAAGTGACTGAGTTAGTTGA+CGG 0.622653 3.3:+73802373 MS.gene03202:CDS
TTTGATTTGGCACTAGAGGA+TGG 0.624229 3.3:-73802081 None:intergenic
ATAGTGAAAGGAAATCGTAT+GGG 0.628856 3.3:-73802550 None:intergenic
ACTTGATTGTATGGGTAGAG+AGG 0.629120 3.3:-73801967 None:intergenic
TTGTTGATGGAGAGTCCACA+TGG 0.630682 3.3:+73802428 MS.gene03202:CDS
GTCACTTGCACAGCTAACAA+TGG 0.634835 3.3:-73802358 None:intergenic
GAGGTTATTCCATTTCACAA+AGG 0.635344 3.3:+73802624 MS.gene03202:CDS
TCATGGGCTAAAATCTCGCG+TGG 0.637561 3.3:+73802463 MS.gene03202:CDS
AAACCTGTGGCAATTCGCAG+TGG 0.640198 3.3:+73803096 MS.gene03202:CDS
TTACCATAAACATCAAACCA+CGG 0.662908 3.3:-73803057 None:intergenic
ATTGCCTCCAAAATCTTGTG+AGG 0.672285 3.3:-73803192 None:intergenic
GCAGCGGAGAACACTTGCGT+AGG 0.674294 3.3:+73802247 MS.gene03202:CDS
ATCGGAGTTCAAAACTACGA+AGG 0.674883 3.3:+73802325 MS.gene03202:CDS
CTAGAACGTTGGTTTCCAAG+TGG 0.676278 3.3:+73802517 MS.gene03202:CDS
TACGCAAGTGTTCTCCGCTG+CGG 0.687317 3.3:-73802245 None:intergenic
TGAGTTAATGAAAAGCCATG+TGG 0.717706 3.3:-73802443 None:intergenic
GCTCTTTGTCCATGCCGCAG+CGG 0.721004 3.3:+73802231 MS.gene03202:CDS
ACCATAAACATCAAACCACG+GGG 0.743607 3.3:-73803055 None:intergenic
TAGCCAATAGCAATTCCCCG+TGG 0.764549 3.3:+73803040 MS.gene03202:CDS
ACTCTCCATCAACAACCGCG+TGG 0.786602 3.3:-73802420 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAAAAAATTTAACAATGTT+TGG - chr3.3:73803000-73803019 None:intergenic 15.0%
!!! ATTTTTTGATCAATTGAATT+TGG - chr3.3:73801995-73802014 None:intergenic 15.0%
!! ATTGGCTAATGTATTATTAT+TGG - chr3.3:73803028-73803047 None:intergenic 20.0%
!!! AAAAAATTGATTTAACTCCA+TGG + chr3.3:73802008-73802027 MS.gene03202:CDS 20.0%
!!! AAAAATTGATTTAACTCCAT+GGG + chr3.3:73802009-73802028 MS.gene03202:CDS 20.0%
!!! GATTCATTTTTTCCATTAAT+CGG + chr3.3:73802307-73802326 MS.gene03202:CDS 20.0%
! ACACGAAGAAGTTTATTATA+TGG + chr3.3:73802768-73802787 MS.gene03202:CDS 25.0%
! TATAATAAACTTCTTCGTGT+AGG - chr3.3:73802768-73802787 None:intergenic 25.0%
! TTCAGATGTTGAATTTGATT+TGG - chr3.3:73802097-73802116 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr3.3:73803066-73803085 MS.gene03202:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG + chr3.3:73803071-73803090 MS.gene03202:CDS 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG + chr3.3:73803070-73803089 MS.gene03202:CDS 25.0%
AAAAATTCAATCCATCAGAG+AGG + chr3.3:73802605-73802624 MS.gene03202:CDS 30.0%
AAATGCTGTTGAAGTTTGTA+GGG + chr3.3:73802846-73802865 MS.gene03202:CDS 30.0%
ATAGTGAAAGGAAATCGTAT+GGG - chr3.3:73802553-73802572 None:intergenic 30.0%
GCATTTCCAAAATAATCTTC+AGG - chr3.3:73802832-73802851 None:intergenic 30.0%
TATAGTGAAAGGAAATCGTA+TGG - chr3.3:73802554-73802573 None:intergenic 30.0%
TTACCATAAACATCAAACCA+CGG - chr3.3:73803060-73803079 None:intergenic 30.0%
! AATTTGGTGACTTGATTGTA+TGG - chr3.3:73801979-73801998 None:intergenic 30.0%
! ATGGCTTTTCATTAACTCAT+GGG + chr3.3:73802447-73802466 MS.gene03202:CDS 30.0%
! ATTTGGTGACTTGATTGTAT+GGG - chr3.3:73801978-73801997 None:intergenic 30.0%
! TTGGAGTGATGATATTTTGT+TGG - chr3.3:73802695-73802714 None:intergenic 30.0%
!! TATGGTAATGATTTTGGATG+GGG + chr3.3:73803072-73803091 MS.gene03202:CDS 30.0%
!!! TAGTTTTGAACTCCGATTAA+TGG - chr3.3:73802322-73802341 None:intergenic 30.0%
AAATAATCTTCAGGCAATGG+TGG - chr3.3:73802823-73802842 None:intergenic 35.0%
AAATGGAATAACCTCTCTGA+TGG - chr3.3:73802619-73802638 None:intergenic 35.0%
AACTCATAACACTAGAACGT+TGG + chr3.3:73802506-73802525 MS.gene03202:CDS 35.0%
ACGTTCTAGTGTTATGAGTT+TGG - chr3.3:73802506-73802525 None:intergenic 35.0%
ACTGAAATTGAAAGCCAACA+TGG + chr3.3:73802660-73802679 MS.gene03202:CDS 35.0%
AGGAAAAGAAGAAGGTAGTA+TGG + chr3.3:73803152-73803171 MS.gene03202:CDS 35.0%
CCAAAATAATCTTCAGGCAA+TGG - chr3.3:73802826-73802845 None:intergenic 35.0%
CGCAGTGGAAATAAAATAAG+TGG + chr3.3:73803111-73803130 MS.gene03202:CDS 35.0%
GAAATGCTGTTGAAGTTTGT+AGG + chr3.3:73802845-73802864 MS.gene03202:CDS 35.0%
GAGGTTATTCCATTTCACAA+AGG + chr3.3:73802624-73802643 MS.gene03202:CDS 35.0%
GGAAAAAATGAATCGACGAT+GGG - chr3.3:73802301-73802320 None:intergenic 35.0%
GGAATGATTACTGTATTTGC+AGG + chr3.3:73803132-73803151 MS.gene03202:CDS 35.0%
GTAGGTGAAATTGTTTGGAA+CGG - chr3.3:73802750-73802769 None:intergenic 35.0%
GTATTTGCAGGAAAAGAAGA+AGG + chr3.3:73803144-73803163 MS.gene03202:CDS 35.0%
GTTTATTATATGGTCCCGAT+AGG + chr3.3:73802778-73802797 MS.gene03202:CDS 35.0%
TACCATAAACATCAAACCAC+GGG - chr3.3:73803059-73803078 None:intergenic 35.0%
TGAGTTAATGAAAAGCCATG+TGG - chr3.3:73802446-73802465 None:intergenic 35.0%
TGGAAAAAATGAATCGACGA+TGG - chr3.3:73802302-73802321 None:intergenic 35.0%
TTCGTGTAGGTGAAATTGTT+TGG - chr3.3:73802755-73802774 None:intergenic 35.0%
! ATAAAGAAGACCCTTTTTGG+TGG - chr3.3:73802059-73802078 None:intergenic 35.0%
! CATGGCTTTTCATTAACTCA+TGG + chr3.3:73802446-73802465 MS.gene03202:CDS 35.0%
! CCATTGCCTGAAGATTATTT+TGG + chr3.3:73802823-73802842 MS.gene03202:CDS 35.0%
! CGATTTTCTCCTTTGTGAAA+TGG - chr3.3:73802636-73802655 None:intergenic 35.0%
! GTGATAAAGAAGACCCTTTT+TGG - chr3.3:73802062-73802081 None:intergenic 35.0%
!! AAAGGTGTGTAAAAAGTGCT+TGG - chr3.3:73802714-73802733 None:intergenic 35.0%
!! AAGCACTTTTTACACACCTT+TGG + chr3.3:73802713-73802732 MS.gene03202:CDS 35.0%
!! TAAAGAAGACCCTTTTTGGT+GGG - chr3.3:73802058-73802077 None:intergenic 35.0%
!! TGAATTTGATTTGGCACTAG+AGG - chr3.3:73802088-73802107 None:intergenic 35.0%
!! TGGTTTTACATTTAACCACG+CGG + chr3.3:73802405-73802424 MS.gene03202:CDS 35.0%
!! TGTGATCTGTTATTTCGAGA+CGG - chr3.3:73802165-73802184 None:intergenic 35.0%
!! TTTTGTGGCTCTATAGTGAA+AGG - chr3.3:73802565-73802584 None:intergenic 35.0%
AAAAATGAATCGACGATGGG+AGG - chr3.3:73802298-73802317 None:intergenic 40.0%
AATCACTACGAGTCTTGGTT+GGG + chr3.3:73802970-73802989 MS.gene03202:CDS 40.0%
AATGAAAGCAGGACAGTTGT+TGG + chr3.3:73802873-73802892 MS.gene03202:CDS 40.0%
ACACAACATCCATGAAGTCA+TGG - chr3.3:73803227-73803246 None:intergenic 40.0%
ACCATAAACATCAAACCACG+GGG - chr3.3:73803058-73803077 None:intergenic 40.0%
ACTAATTGCACCCACCAAAA+AGG + chr3.3:73802045-73802064 MS.gene03202:CDS 40.0%
ACTTGATTGTATGGGTAGAG+AGG - chr3.3:73801970-73801989 None:intergenic 40.0%
ATCGGAGTTCAAAACTACGA+AGG + chr3.3:73802325-73802344 MS.gene03202:CDS 40.0%
ATGGGATGTTGAATACCACT+TGG - chr3.3:73802535-73802554 None:intergenic 40.0%
ATTGCCTCCAAAATCTTGTG+AGG - chr3.3:73803195-73803214 None:intergenic 40.0%
ATTTAACTCCATGGGATCTC+CGG + chr3.3:73802017-73802036 MS.gene03202:CDS 40.0%
CAAGTGACTGAGTTAGTTGA+CGG + chr3.3:73802373-73802392 MS.gene03202:CDS 40.0%
CTAATTGCACCCACCAAAAA+GGG + chr3.3:73802046-73802065 MS.gene03202:CDS 40.0%
TGAAGAATCACTACGAGTCT+TGG + chr3.3:73802965-73802984 MS.gene03202:CDS 40.0%
TGTAGGGTTACAATGAAAGC+AGG + chr3.3:73802862-73802881 MS.gene03202:CDS 40.0%
TTAGTTGACGGCATCTTCAT+TGG + chr3.3:73802385-73802404 MS.gene03202:CDS 40.0%
TTGAAAGCCAACATGGAGAT+TGG + chr3.3:73802667-73802686 MS.gene03202:CDS 40.0%
! AACCTTGATCTAGCTCCTAT+CGG - chr3.3:73802796-73802815 None:intergenic 40.0%
! GTGTCTTCCTCACAAGATTT+TGG + chr3.3:73803185-73803204 MS.gene03202:CDS 40.0%
! TCATCAGAGTCGTTCTTTTG+TGG - chr3.3:73802580-73802599 None:intergenic 40.0%
! TCTTCCTCACAAGATTTTGG+AGG + chr3.3:73803188-73803207 MS.gene03202:CDS 40.0%
!! CACAAGATTTTGGAGGCAAT+GGG + chr3.3:73803195-73803214 MS.gene03202:CDS 40.0%
!! TCACAAGATTTTGGAGGCAA+TGG + chr3.3:73803194-73803213 MS.gene03202:CDS 40.0%
!! TTTGATTTGGCACTAGAGGA+TGG - chr3.3:73802084-73802103 None:intergenic 40.0%
!! TTTTGGTGGGTGCAATTAGT+AGG - chr3.3:73802045-73802064 None:intergenic 40.0%
AAACCACGGGGAATTGCTAT+TGG - chr3.3:73803046-73803065 None:intergenic 45.0%
ACCAGCATTGTTGCATGTGA+CGG - chr3.3:73802209-73802228 None:intergenic 45.0%
ACTTGCGTAGGTGACATACT+TGG + chr3.3:73802259-73802278 MS.gene03202:CDS 45.0%
GAATCACTACGAGTCTTGGT+TGG + chr3.3:73802969-73802988 MS.gene03202:CDS 45.0%
GACAGTTGTTGGAAGATGGT+GGG + chr3.3:73802884-73802903 MS.gene03202:CDS 45.0%
GGGAAATGACCATGACTTCA+TGG + chr3.3:73803215-73803234 MS.gene03202:CDS 45.0%
GTCACTTGCACAGCTAACAA+TGG - chr3.3:73802361-73802380 None:intergenic 45.0%
GTTGGTACCAATCTCCATGT+TGG - chr3.3:73802677-73802696 None:intergenic 45.0%
TCCCCGTGGTTTGATGTTTA+TGG + chr3.3:73803054-73803073 MS.gene03202:CDS 45.0%
TGGGTGCAATTAGTAGGTAC+CGG - chr3.3:73802039-73802058 None:intergenic 45.0%
TTGTATGGGTAGAGAGGACT+TGG - chr3.3:73801964-73801983 None:intergenic 45.0%
TTTAACCACGCGGTTGTTGA+TGG + chr3.3:73802415-73802434 MS.gene03202:CDS 45.0%
! AAGTGGTGGGAAAAAGGCAA+GGG - chr3.3:73802137-73802156 None:intergenic 45.0%
! ACCTTGATCTAGCTCCTATC+GGG - chr3.3:73802795-73802814 None:intergenic 45.0%
! GCATGTGACGGAACATGAAA+TGG - chr3.3:73802197-73802216 None:intergenic 45.0%
! TTGTTGATGGAGAGTCCACA+TGG + chr3.3:73802428-73802447 MS.gene03202:CDS 45.0%
! TTTTGGATGGGGAAAACCTG+TGG + chr3.3:73803083-73803102 MS.gene03202:CDS 45.0%
!! CTAGAACGTTGGTTTCCAAG+TGG + chr3.3:73802517-73802536 MS.gene03202:CDS 45.0%
AAACCTGTGGCAATTCGCAG+TGG + chr3.3:73803096-73803115 MS.gene03202:CDS 50.0%
GCAGGACAGTTGTTGGAAGA+TGG + chr3.3:73802880-73802899 MS.gene03202:CDS 50.0%
GGACAGTTGTTGGAAGATGG+TGG + chr3.3:73802883-73802902 MS.gene03202:CDS 50.0%
TAGCCAATAGCAATTCCCCG+TGG + chr3.3:73803040-73803059 MS.gene03202:CDS 50.0%
TCCCGATAGGAGCTAGATCA+AGG + chr3.3:73802791-73802810 MS.gene03202:CDS 50.0%
TCCGTCACATGCAACAATGC+TGG + chr3.3:73802205-73802224 MS.gene03202:CDS 50.0%
TTTCCACTGCGAATTGCCAC+AGG - chr3.3:73803102-73803121 None:intergenic 50.0%
! CAAGTGGTGGGAAAAAGGCA+AGG - chr3.3:73802138-73802157 None:intergenic 50.0%
! TCATGGGCTAAAATCTCGCG+TGG + chr3.3:73802463-73802482 MS.gene03202:CDS 50.0%
!! GAAGATGGTGGGCTTGGTAA+AGG + chr3.3:73802895-73802914 MS.gene03202:CDS 50.0%
!! TGGGCTTGGTAAAGGTGCTT+GGG + chr3.3:73802903-73802922 MS.gene03202:CDS 50.0%
!! TTGTTGGAAGATGGTGGGCT+TGG + chr3.3:73802889-73802908 MS.gene03202:CDS 50.0%
ACTCTCCATCAACAACCGCG+TGG - chr3.3:73802423-73802442 None:intergenic 55.0%
ATCGACGATGGGAGGAACGT+AGG - chr3.3:73802290-73802309 None:intergenic 55.0%
ATTTCGAGACGGCCTGCAAG+TGG - chr3.3:73802154-73802173 None:intergenic 55.0%
TACGCAAGTGTTCTCCGCTG+CGG - chr3.3:73802248-73802267 None:intergenic 55.0%
! AACGGATCACAGAGCGCCAA+AGG - chr3.3:73802732-73802751 None:intergenic 55.0%
! GCCTGCAAGTGGTGGGAAAA+AGG - chr3.3:73802143-73802162 None:intergenic 55.0%
! GCCTTTTTCCCACCACTTGC+AGG + chr3.3:73802139-73802158 MS.gene03202:CDS 55.0%
!! AGTAGGTACCGGAGATCCCA+TGG - chr3.3:73802028-73802047 None:intergenic 55.0%
!! GTGGGCTTGGTAAAGGTGCT+TGG + chr3.3:73802902-73802921 MS.gene03202:CDS 55.0%
AAGTGTTCTCCGCTGCGGCA+TGG - chr3.3:73802243-73802262 None:intergenic 60.0%
GCAGCGGAGAACACTTGCGT+AGG + chr3.3:73802247-73802266 MS.gene03202:CDS 60.0%
GCTCTTTGTCCATGCCGCAG+CGG + chr3.3:73802231-73802250 MS.gene03202:CDS 60.0%
CGAGACGGCCTGCAAGTGGT+GGG - chr3.3:73802150-73802169 None:intergenic 65.0%
TCGAGACGGCCTGCAAGTGG+TGG - chr3.3:73802151-73802170 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 73801950 73803254 73801950 ID=MS.gene03202
chr3.3 mRNA 73801950 73803254 73801950 ID=MS.gene03202.t1;Parent=MS.gene03202
chr3.3 exon 73801950 73803254 73801950 ID=MS.gene03202.t1.exon1;Parent=MS.gene03202.t1
chr3.3 CDS 73801950 73803254 73801950 ID=cds.MS.gene03202.t1;Parent=MS.gene03202.t1
Gene Sequence

>MS.gene03202

ATGGCTGCAATCCAAGTCCTCTCTACCCATACAATCAAGTCACCAAATTCAATTGATCAAAAAATTGATTTAACTCCATGGGATCTCCGGTACCTACTAATTGCACCCACCAAAAAGGGTCTTCTTTATCACCATCCTCTAGTGCCAAATCAAATTCAACATCTGAAACACTCTCTATCTTCTGCCCTTGCCTTTTTCCCACCACTTGCAGGCCGTCTCGAAATAACAGATCACAAAGACAACACCATTTCATGTTCCGTCACATGCAACAATGCTGGTGCGCTCTTTGTCCATGCCGCAGCGGAGAACACTTGCGTAGGTGACATACTTGGATGCACCTACGTTCCTCCCATCGTCGATTCATTTTTTCCATTAATCGGAGTTCAAAACTACGAAGGCACGTCACAACCATTGTTAGCTGTGCAAGTGACTGAGTTAGTTGACGGCATCTTCATTGGTTTTACATTTAACCACGCGGTTGTTGATGGAGAGTCCACATGGCTTTTCATTAACTCATGGGCTAAAATCTCGCGTGGTTGTTGCAATCAAGTATCCAAACTCATAACACTAGAACGTTGGTTTCCAAGTGGTATTCAACATCCCATACGATTTCCTTTCACTATAGAGCCACAAAAGAACGACTCTGATGATAACGAAAAATTCAATCCATCAGAGAGGTTATTCCATTTCACAAAGGAGAAAATCGCTCAACTGAAATTGAAAGCCAACATGGAGATTGGTACCAACAAAATATCATCACTCCAAGCACTTTTTACACACCTTTGGCGCTCTGTGATCCGTTCCAAACAATTTCACCTACACGAAGAAGTTTATTATATGGTCCCGATAGGAGCTAGATCAAGGTTTGTTCCACCATTGCCTGAAGATTATTTTGGAAATGCTGTTGAAGTTTGTAGGGTTACAATGAAAGCAGGACAGTTGTTGGAAGATGGTGGGCTTGGTAAAGGTGCTTGGGCGATACACAAAATGATTTCTTTGCAGTCAAATGAGAAGTTGAAGAATCACTACGAGTCTTGGTTGGGAAATCCAAACATTGTTAAATTTTTTAGTGCGACCAATAATAATACATTAGCCAATAGCAATTCCCCGTGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAACCTGTGGCAATTCGCAGTGGAAATAAAATAAGTGGAATGATTACTGTATTTGCAGGAAAAGAAGAAGGTAGTATGGACTTTCAAGTGTGTCTTCCTCACAAGATTTTGGAGGCAATGGGAAATGACCATGACTTCATGGATGTTGTGTCTATTTAA
Protein sequence

>MS.gene03202.t1

MAAIQVLSTHTIKSPNSIDQKIDLTPWDLRYLLIAPTKKGLLYHHPLVPNQIQHLKHSLSSALAFFPPLAGRLEITDHKDNTISCSVTCNNAGALFVHAAAENTCVGDILGCTYVPPIVDSFFPLIGVQNYEGTSQPLLAVQVTELVDGIFIGFTFNHAVVDGESTWLFINSWAKISRGCCNQVSKLITLERWFPSGIQHPIRFPFTIEPQKNDSDDNEKFNPSERLFHFTKEKIAQLKLKANMEIGTNKISSLQALFTHLWRSVIRSKQFHLHEEVYYMVPIGARSRFVPPLPEDYFGNAVEVCRVTMKAGQLLEDGGLGKGAWAIHKMISLQSNEKLKNHYESWLGNPNIVKFFSATNNNTLANSNSPWFDVYGNDFGWGKPVAIRSGNKISGMITVFAGKEEGSMDFQVCLPHKILEAMGNDHDFMDVVSI