AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38977

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38977.t1 MTR_0190s0020 94.131 443 25 1 1 442 1 443 0.0 868
MS.gene38977.t1 MTR_8g041770 71.041 442 126 2 1 441 1 441 0.0 637
MS.gene38977.t1 MTR_4g022800 60.177 452 159 6 1 441 1 442 0.0 540
MS.gene38977.t1 MTR_4g022720 60.225 445 163 6 1 441 1 435 0.0 531
MS.gene38977.t1 MTR_4g022670 58.758 451 166 6 1 441 1 441 0.0 530
MS.gene38977.t1 MTR_7g026860 59.292 452 159 7 1 441 1 438 0.0 527
MS.gene38977.t1 MTR_3g088420 59.819 443 166 5 1 441 1 433 0.0 525
MS.gene38977.t1 MTR_4g022825 57.965 452 167 7 1 441 1 440 0.0 521
MS.gene38977.t1 MTR_3g026310 57.642 458 167 6 1 441 1 448 0.0 516
MS.gene38977.t1 MTR_4g094835 58.036 448 171 8 1 437 1 442 4.54e-179 508
MS.gene38977.t1 MTR_4g094842 55.457 449 187 5 1 439 1 446 1.08e-172 491
MS.gene38977.t1 MTR_4g094838 54.688 448 169 8 4 437 1 428 7.75e-165 471
MS.gene38977.t1 MTR_4g094832 54.460 426 173 7 28 437 6 426 1.61e-158 455
MS.gene38977.t1 MTR_4g022760 52.222 450 153 6 1 441 1 397 2.08e-155 446
MS.gene38977.t1 MTR_4g094828 50.877 456 203 9 1 441 1 450 1.08e-153 443
MS.gene38977.t1 MTR_8g079710 51.802 444 194 9 1 432 1 436 1.93e-153 442
MS.gene38977.t1 MTR_4g022290 51.322 454 195 10 1 439 1 443 2.01e-150 435
MS.gene38977.t1 MTR_6g016815 45.721 444 161 9 1 441 15 381 1.02e-117 349
MS.gene38977.t1 MTR_3g101900 39.765 425 240 9 28 441 29 448 5.72e-95 293
MS.gene38977.t1 MTR_3g101930 39.229 441 246 10 17 440 19 454 2.11e-92 287
MS.gene38977.t1 MTR_3g102010 40.047 427 239 9 28 441 29 451 9.55e-91 283
MS.gene38977.t1 MTR_3g102020 39.019 428 243 9 28 441 28 451 4.36e-88 276
MS.gene38977.t1 MTR_8g040760 64.019 214 74 2 1 213 1 212 1.09e-87 266
MS.gene38977.t1 MTR_3g101970 38.061 423 238 9 28 440 28 436 4.46e-87 273
MS.gene38977.t1 MTR_8g040780 58.986 217 86 2 223 436 11 227 2.57e-86 264
MS.gene38977.t1 MTR_3g101960 38.626 422 242 10 28 436 28 445 7.16e-86 270
MS.gene38977.t1 MTR_3g070170 31.884 483 264 9 2 441 10 470 1.28e-73 239
MS.gene38977.t1 MTR_5g081960 32.700 474 250 9 22 437 20 482 7.44e-68 224
MS.gene38977.t1 MTR_4g015180 34.127 504 248 15 2 441 4 487 3.38e-67 223
MS.gene38977.t1 MTR_7g026850 62.424 165 58 2 24 188 3 163 1.10e-64 206
MS.gene38977.t1 MTR_4g094825 51.500 200 87 4 17 209 80 276 2.52e-57 194
MS.gene38977.t1 MTR_4g094825 53.642 151 58 2 292 441 252 391 4.31e-47 167
MS.gene38977.t1 MTR_7g026770 50.794 189 70 3 149 335 132 299 3.16e-54 183
MS.gene38977.t1 MTR_6g034495 70.161 124 32 2 73 191 44 167 9.81e-53 174
MS.gene38977.t1 MTR_6g045553 30.134 448 265 10 28 437 34 471 2.25e-49 175
MS.gene38977.t1 MTR_3g102030 33.663 303 166 7 98 395 48 320 3.79e-46 162
MS.gene38977.t1 MTR_6g034485 54.762 126 56 1 194 319 1 125 5.06e-46 155
MS.gene38977.t1 MTR_7g026830 66.667 84 28 0 90 173 14 97 5.22e-32 117
MS.gene38977.t1 MTR_4g007540 24.757 412 273 13 41 432 34 428 2.47e-31 125
MS.gene38977.t1 MTR_8g096530 31.287 342 207 12 61 390 57 382 8.34e-31 123
MS.gene38977.t1 MTR_3g098980 28.684 380 238 14 63 428 66 426 4.43e-30 121
MS.gene38977.t1 MTR_2g105330 25.123 406 270 12 41 428 34 423 5.03e-30 121
MS.gene38977.t1 MTR_2g064495 25.123 406 270 12 41 428 34 423 9.42e-30 120
MS.gene38977.t1 MTR_3g026950 27.553 421 243 17 47 426 38 437 4.91e-29 118
MS.gene38977.t1 MTR_8g075610 26.166 386 250 13 63 431 47 414 6.01e-29 118
MS.gene38977.t1 MTR_3g049150 27.273 418 229 16 60 431 52 440 3.06e-26 110
MS.gene38977.t1 MTR_7g026780 50.450 111 30 2 331 441 26 111 2.86e-25 100
MS.gene38977.t1 MTR_4g097090 26.923 390 241 16 63 422 65 440 1.74e-23 102
MS.gene38977.t1 MTR_4g127650 25.195 385 253 13 63 422 66 440 4.13e-23 101
MS.gene38977.t1 MTR_7g027175 25.385 390 257 11 58 432 63 433 1.17e-22 100
MS.gene38977.t1 MTR_7g084940 29.155 343 213 11 61 390 67 392 1.93e-22 99.8
MS.gene38977.t1 MTR_4g022780 37.097 186 76 6 201 375 2 157 2.10e-22 94.4
MS.gene38977.t1 MTR_1g040175 26.031 388 248 17 63 430 64 432 3.62e-22 99.0
MS.gene38977.t1 MTR_4g013050 25.064 391 237 12 61 432 51 404 6.46e-22 97.8
MS.gene38977.t1 MTR_7g026800 38.961 154 63 4 224 375 32 156 1.30e-21 92.4
MS.gene38977.t1 MTR_7g026870 56.000 75 33 0 276 350 77 151 2.40e-21 90.5
MS.gene38977.t1 MTR_7g086000 26.044 407 247 15 63 431 60 450 4.77e-21 95.5
MS.gene38977.t1 MTR_7g014330 27.818 417 227 17 26 394 23 413 6.16e-21 95.1
MS.gene38977.t1 MTR_3g086020 25.768 423 276 13 28 441 22 415 2.33e-20 93.2
MS.gene38977.t1 MTR_2g015430 26.479 355 233 11 48 393 51 386 3.71e-20 92.8
MS.gene38977.t1 MTR_7g014250 26.859 417 231 17 26 394 23 413 1.53e-19 90.9
MS.gene38977.t1 MTR_6g015315 27.129 317 185 12 146 429 140 443 2.56e-19 90.5
MS.gene38977.t1 MTR_1g040215 24.694 409 243 15 30 405 23 399 3.92e-19 89.7
MS.gene38977.t1 MTR_1g040195 27.368 380 219 17 77 428 55 405 4.38e-19 89.4
MS.gene38977.t1 MTR_1g053315 26.377 345 226 15 63 391 78 410 5.12e-19 89.4
MS.gene38977.t1 MTR_7g114520 24.533 375 257 12 46 407 49 410 1.20e-18 88.2
MS.gene38977.t1 MTR_3g087640 26.709 468 284 22 5 428 10 462 2.32e-18 87.4
MS.gene38977.t1 MTR_2g089755 25.980 408 249 17 29 394 37 433 3.73e-18 87.0
MS.gene38977.t1 MTR_8g079980 24.745 392 225 15 55 414 51 404 4.86e-18 86.3
MS.gene38977.t1 MTR_1g081660 24.378 402 244 17 29 400 31 402 5.32e-18 86.3
MS.gene38977.t1 MTR_6g015320 27.597 308 192 10 146 428 143 444 5.64e-18 86.3
MS.gene38977.t1 MTR_2g089650 25.175 429 274 15 26 418 26 443 1.25e-17 85.1
MS.gene38977.t1 MTR_6g015850 25.570 395 256 14 60 429 67 448 6.24e-17 83.2
MS.gene38977.t1 MTR_3g049140 26.749 243 145 8 211 431 6 237 1.03e-16 80.1
MS.gene38977.t1 MTR_7g015860 25.464 377 206 16 63 391 64 413 1.04e-16 82.4
MS.gene38977.t1 MTR_7g114550 23.364 428 273 17 30 425 24 428 1.26e-16 82.0
MS.gene38977.t1 MTR_7g085930 25.123 406 251 15 60 426 56 447 2.85e-16 81.3
MS.gene38977.t1 MTR_3g112180 25.515 388 238 15 25 389 29 388 3.08e-16 80.9
MS.gene38977.t1 MTR_5g026320 24.672 458 293 18 4 436 18 448 3.13e-16 80.9
MS.gene38977.t1 MTR_3g465980 26.199 271 183 6 63 323 68 331 3.22e-16 80.9
MS.gene38977.t1 MTR_3g465990 26.838 272 180 8 63 323 68 331 1.22e-15 79.3
MS.gene38977.t1 MTR_1g040315 24.582 419 241 18 30 428 23 386 1.38e-14 75.9
MS.gene38977.t1 MTR_7g114470 22.673 419 289 13 30 425 25 431 2.67e-14 75.1
MS.gene38977.t1 MTR_7g085990 25.574 305 202 9 60 344 52 351 5.25e-14 74.3
MS.gene38977.t1 MTR_4g055170 26.367 311 204 9 52 350 58 355 5.48e-14 73.9
MS.gene38977.t1 MTR_2g089670 25.773 388 230 12 43 390 60 429 8.91e-14 73.6
MS.gene38977.t1 MTR_7g014360 29.104 268 157 11 146 394 90 343 1.28e-13 72.4
MS.gene38977.t1 MTR_7g026880 55.556 54 22 1 388 441 2 53 5.88e-13 63.5
MS.gene38977.t1 MTR_7g084910 27.799 259 167 8 61 307 68 318 8.35e-13 70.5
MS.gene38977.t1 MTR_7g056087 23.282 262 183 4 77 323 72 330 3.35e-11 65.5
MS.gene38977.t1 MTR_2g089735 24.658 438 271 21 37 430 31 453 3.53e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38977.t1 AT5G67150 40.766 444 247 10 4 437 5 442 4.04e-107 324
MS.gene38977.t1 AT3G50300 40.596 436 241 11 1 426 1 428 2.46e-101 310
MS.gene38977.t1 AT3G50280 37.668 446 264 9 1 438 1 440 1.09e-99 305
MS.gene38977.t1 AT5G67160 39.765 425 237 8 22 441 22 432 1.09e-95 295
MS.gene38977.t1 AT3G50270 37.808 447 265 9 4 441 6 448 7.02e-95 293
MS.gene38977.t1 AT5G42830 38.584 438 248 11 18 441 19 449 4.60e-89 278
MS.gene38977.t1 AT5G38130 38.391 435 242 13 1 422 21 442 9.17e-85 267
MS.gene38977.t1 AT5G07860 38.140 430 238 10 28 440 30 448 2.22e-83 263
MS.gene38977.t1 AT5G07850 38.889 432 238 11 28 441 30 453 2.87e-82 261
MS.gene38977.t1 AT5G07870 36.281 441 245 10 28 441 30 461 4.76e-76 245
MS.gene38977.t1 AT5G01210 35.792 461 244 9 22 441 20 469 2.32e-75 243
MS.gene38977.t1 AT2G39980 33.745 486 257 10 9 441 7 480 2.92e-74 241
MS.gene38977.t1 AT3G50280 38.141 312 185 5 133 438 1 310 3.37e-70 225
MS.gene38977.t1 AT5G23940 32.230 453 281 10 4 437 3 448 3.88e-67 222
MS.gene38977.t1 AT3G50290 41.573 267 147 5 130 388 4 269 3.65e-53 179
MS.gene38977.t1 AT5G48930 26.420 405 261 12 41 425 34 421 7.31e-30 120
MS.gene38977.t1 AT3G48720 27.020 396 260 12 47 429 44 423 1.54e-28 117
MS.gene38977.t1 AT2G19070 26.747 415 264 14 47 432 42 445 1.25e-26 112
MS.gene38977.t1 AT5G41040 25.500 400 256 13 55 433 59 437 2.39e-25 108
MS.gene38977.t1 AT5G41040 25.628 398 254 13 55 431 75 451 3.31e-25 107
MS.gene38977.t1 AT2G40230 27.249 389 257 13 20 395 16 391 4.33e-24 104
MS.gene38977.t1 AT3G29590 25.727 447 298 13 2 428 6 438 3.41e-23 102
MS.gene38977.t1 AT5G63560 24.311 399 263 15 56 439 52 426 2.81e-22 99.0
MS.gene38977.t1 AT1G28680 28.519 270 162 9 140 393 135 389 1.53e-21 97.1
MS.gene38977.t1 AT1G28680 31.937 191 120 5 140 323 135 322 1.68e-20 93.2
MS.gene38977.t1 AT3G03480 25.404 433 263 17 30 428 33 439 9.18e-20 91.7
MS.gene38977.t1 AT3G29635 25.349 430 280 16 26 426 26 443 1.12e-19 91.7
MS.gene38977.t1 AT3G26040 26.018 442 267 16 28 441 20 429 7.66e-19 89.0
MS.gene38977.t1 AT1G24430 25.495 455 288 19 4 441 5 425 3.06e-18 87.0
MS.gene38977.t1 AT5G23970 24.508 457 280 16 4 437 3 417 1.49e-17 84.7
MS.gene38977.t1 AT3G30280 23.341 437 290 19 25 441 25 436 7.76e-17 82.8
MS.gene38977.t1 AT5G61160 24.679 312 197 10 143 428 139 438 9.50e-17 82.4
MS.gene38977.t1 AT1G24420 24.603 378 253 13 73 441 75 429 4.25e-16 80.5
MS.gene38977.t1 AT1G24420 24.603 378 253 13 73 441 75 429 4.25e-16 80.5
MS.gene38977.t1 AT4G15390 22.326 430 296 14 25 437 27 435 5.77e-16 80.1
MS.gene38977.t1 AT4G15390 22.326 430 296 14 25 437 27 435 5.77e-16 80.1
MS.gene38977.t1 AT1G78990 26.685 371 219 16 48 391 52 396 8.58e-16 79.7
MS.gene38977.t1 AT5G39090 27.541 305 177 13 143 420 140 427 8.58e-16 79.7
MS.gene38977.t1 AT5G07080 25.000 280 169 10 64 321 71 331 1.46e-15 79.0
MS.gene38977.t1 AT2G23510 24.513 359 216 14 63 393 69 400 5.98e-15 77.0
MS.gene38977.t1 AT1G32910 26.316 361 212 15 61 391 63 399 2.36e-14 75.1
MS.gene38977.t1 AT5G47950 22.738 431 281 15 26 441 26 419 8.23e-14 73.6
MS.gene38977.t1 AT1G65450 25.852 352 218 15 61 390 70 400 8.72e-14 73.6
MS.gene38977.t1 AT5G16410 24.869 382 231 17 63 415 91 445 2.33e-13 72.4
MS.gene38977.t1 AT3G47170 22.526 293 203 9 50 321 55 344 5.30e-13 71.2
MS.gene38977.t1 AT1G31490 23.214 392 268 14 60 436 59 432 3.79e-12 68.2
MS.gene38977.t1 AT2G25150 24.823 282 176 11 147 407 148 414 1.23e-11 67.0

Find 94 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCTTGGTTTGATGTTTA+TGG 0.170784 8.4:+49099044 MS.gene38977:CDS
TAGAAATGATCCTGAGTTTA+TGG 0.174155 8.4:+49099211 MS.gene38977:CDS
TAGAAGAAAGAGAGTCTTTA+AGG 0.197250 8.4:-49098107 None:intergenic
ATGTCTTCCTTGTGAAATTT+TGG 0.200982 8.4:+49099181 MS.gene38977:CDS
CCGTTGCCTGAGGATTATTT+TGG 0.234642 8.4:+49098816 MS.gene38977:CDS
TTAATCGACGGCATCTTTAT+TGG 0.260930 8.4:+49098381 MS.gene38977:CDS
AACAGTGATTCATTAGTAAT+TGG 0.271903 8.4:+49099017 MS.gene38977:CDS
GTGAAATTCGTGCTGGATAT+TGG 0.281578 8.4:+49098771 MS.gene38977:CDS
GAGAGTATTTCATTTCAAAA+AGG 0.290139 8.4:+49098617 MS.gene38977:CDS
TGGGCTTGGTAAGGGCTCTT+TGG 0.294679 8.4:+49098896 MS.gene38977:CDS
GATGTTTATGGTAATGATTT+TGG 0.307637 8.4:+49099056 MS.gene38977:CDS
ACGATCAAATGTTGGAAGTT+TGG 0.316033 8.4:-49098496 None:intergenic
AAAATTCAAAGCAAACTTAG+AGG 0.330646 8.4:+49098656 MS.gene38977:CDS
GATCTTCAATTCCTCCCATT+TGG 0.358724 8.4:+49098018 MS.gene38977:CDS
TATTGTGAAAGGAAATCTAA+TGG 0.359609 8.4:-49098544 None:intergenic
TTGAAATGAAATACTCTCTC+TGG 0.360829 8.4:-49098612 None:intergenic
GAAGATCCCATGGGGTTAGA+TGG 0.378133 8.4:-49098002 None:intergenic
GTTAACCACGTGATTGTTGA+TGG 0.384791 8.4:+49098411 MS.gene38977:CDS
GGTGATAAAGAAGCCCCTTT+TGG 0.389061 8.4:-49098047 None:intergenic
AAAGAGAGTCTTTAAGGTGT+TGG 0.398134 8.4:-49098101 None:intergenic
GGAAGATGGTGGGCTTGGTA+AGG 0.403175 8.4:+49098887 MS.gene38977:CDS
CCCTTTGTTGTTGCACAAGA+TGG 0.408570 8.4:-49098202 None:intergenic
CATTAAAGCCGGTGACTTGT+TGG 0.413794 8.4:+49098866 MS.gene38977:CDS
CGAATTTCACTTCTGCTTGT+GGG 0.422467 8.4:-49098758 None:intergenic
TTGTTGATGGTACGTCGTTT+TGG 0.426327 8.4:+49098424 MS.gene38977:CDS
ACGAATTTCACTTCTGCTTG+TGG 0.426619 8.4:-49098759 None:intergenic
CCCATTTGGATTCAACCAAA+AGG 0.433362 8.4:+49098032 MS.gene38977:CDS
ATTGTGAAAGGAAATCTAAT+GGG 0.441170 8.4:-49098543 None:intergenic
CCATTTGGATTCAACCAAAA+GGG 0.443246 8.4:+49098033 MS.gene38977:CDS
CGTCGATTAACTCTGTTACT+TGG 0.452112 8.4:-49098368 None:intergenic
TTTGGCATTTCATCAATTCA+TGG 0.452731 8.4:+49098442 MS.gene38977:CDS
TAGTAATTGGTGGTTCTCCT+TGG 0.455174 8.4:+49099030 MS.gene38977:CDS
CAACTTCTCATCTGTGTGCA+AGG 0.466084 8.4:-49098937 None:intergenic
CGTTACACAAGCCGATTAGT+AGG 0.472331 8.4:-49099243 None:intergenic
GAAGATGGTGGGCTTGGTAA+GGG 0.475598 8.4:+49098888 MS.gene38977:CDS
AGTTGTGGAGAGGACTTTGA+TGG 0.477018 8.4:-49097929 None:intergenic
AACTTCCAACATTTGATCGT+TGG 0.480962 8.4:+49098499 MS.gene38977:CDS
AGTGATTCATTAGTAATTGG+TGG 0.482749 8.4:+49099020 MS.gene38977:CDS
CCATCTTGTGCAACAACAAA+GGG 0.484933 8.4:+49098202 MS.gene38977:CDS
TTGGCATTTCATCAATTCAT+GGG 0.489109 8.4:+49098443 MS.gene38977:CDS
AATGAGGATAATTATGAAGA+AGG 0.489922 8.4:+49098576 MS.gene38977:CDS
CACAAGGAAGACATACTTCA+AGG 0.496352 8.4:-49099172 None:intergenic
TGAATAGGAGGATGATAAGT+GGG 0.498813 8.4:-49098279 None:intergenic
CACAATAGAGTCACGAAATG+AGG 0.502975 8.4:+49098560 MS.gene38977:CDS
GTGAATAGGAGGATGATAAG+TGG 0.505672 8.4:-49098280 None:intergenic
TTCAAAGCAAACTTAGAGGT+TGG 0.511060 8.4:+49098660 MS.gene38977:CDS
TGCTGCATGTATTGTAGTTG+TGG 0.511804 8.4:-49097944 None:intergenic
TTGTTCTATCTAATTCGAAA+GGG 0.519195 8.4:-49098071 None:intergenic
TTTCGTGACTCTATTGTGAA+AGG 0.529927 8.4:-49098555 None:intergenic
TTTGTTCTATCTAATTCGAA+AGG 0.534586 8.4:-49098072 None:intergenic
GAAAACCAACGATCAAATGT+TGG 0.535798 8.4:-49098504 None:intergenic
TTGTTGGAAGATGGTGGGCT+TGG 0.536853 8.4:+49098882 MS.gene38977:CDS
GTGAGCGAAAATTGCTTGCA+AGG 0.538924 8.4:-49098700 None:intergenic
TTCTATCTAATTCGAAAGGG+TGG 0.542163 8.4:-49098068 None:intergenic
AGCAGAAGTGAAATTCGTGC+TGG 0.545585 8.4:+49098764 MS.gene38977:CDS
CATTTGGATTCAACCAAAAG+GGG 0.560740 8.4:+49098034 MS.gene38977:CDS
GGGAGGAATTGAAGATCCCA+TGG 0.563488 8.4:-49098012 None:intergenic
GGAAAGATTTATGTGTATGC+GGG 0.563929 8.4:+49099128 MS.gene38977:CDS
ATACCAGAGGTGACAAAACT+CGG 0.565166 8.4:-49098990 None:intergenic
ACCATCTTCCAACAAGTCAC+CGG 0.565694 8.4:-49098874 None:intergenic
GCATTACCAAAATAATCCTC+AGG 0.566644 8.4:-49098822 None:intergenic
TGTGTTGTTAGCATTAAAGC+CGG 0.567065 8.4:+49098855 MS.gene38977:CDS
GCCGGTGACTTGTTGGAAGA+TGG 0.571350 8.4:+49098873 MS.gene38977:CDS
TATCTTGAGACGACCTGTGA+AGG 0.574633 8.4:-49098148 None:intergenic
CCAAAATAATCCTCAGGCAA+CGG 0.577247 8.4:-49098816 None:intergenic
TCCATCTTGTGCAACAACAA+AGG 0.578209 8.4:+49098201 MS.gene38977:CDS
AATTACTAATGAATCACTGT+TGG 0.580439 8.4:-49099015 None:intergenic
GGCAACGGAGGAATCAACCT+TGG 0.584288 8.4:-49098801 None:intergenic
ATAAGTGGGTTTAACGATGT+CGG 0.584805 8.4:-49098265 None:intergenic
GTGACTTGTTGGAAGATGGT+GGG 0.589621 8.4:+49098877 MS.gene38977:CDS
GTTGATTCCTCCGTTGCCTG+AGG 0.590687 8.4:+49098806 MS.gene38977:CDS
GGTGACTTGTTGGAAGATGG+TGG 0.597865 8.4:+49098876 MS.gene38977:CDS
GTGTATGCGGGTGTAGAAGA+TGG 0.601730 8.4:+49099140 MS.gene38977:CDS
ATACAATCCATCTAACCCCA+TGG 0.603547 8.4:+49097995 MS.gene38977:CDS
GCTACGGCATGAACAAAGAG+TGG 0.606907 8.4:-49098225 None:intergenic
TACAATCCATCTAACCCCAT+GGG 0.610598 8.4:+49097996 MS.gene38977:CDS
TTTGGTTGAATCCAAATGGG+AGG 0.611475 8.4:-49098029 None:intergenic
GTTACTTGGACTGCAAGTAA+CGG 0.617187 8.4:-49098354 None:intergenic
TGGAAAGATTTATGTGTATG+CGG 0.617965 8.4:+49099127 MS.gene38977:CDS
ATCTTGAGACGACCTGTGAA+GGG 0.618770 8.4:-49098147 None:intergenic
TCATGGGCTGAAATCTCGAA+AGG 0.621563 8.4:+49098459 MS.gene38977:CDS
GACACAACATCCATAAACTC+AGG 0.640217 8.4:-49099221 None:intergenic
GGAGGAATTGAAGATCCCAT+GGG 0.641441 8.4:-49098011 None:intergenic
AAATAATCCTCAGGCAACGG+AGG 0.648191 8.4:-49098813 None:intergenic
ATTGCCTCCAAAATTTCACA+AGG 0.652126 8.4:-49099188 None:intergenic
ATCACTGTTGGCCATACCAG+AGG 0.652147 8.4:-49099003 None:intergenic
CAAGTAACAGAGTTAATCGA+CGG 0.666552 8.4:+49098369 MS.gene38977:CDS
TTACCATAAACATCAAACCA+AGG 0.678732 8.4:-49099047 None:intergenic
CATGTATTGTAGTTGTGGAG+AGG 0.679095 8.4:-49097939 None:intergenic
TGGATATTGGAATTAGACCA+AGG 0.680206 8.4:+49098784 MS.gene38977:CDS
GGGTGTAGAAGATGGTGGCA+TGG 0.728496 8.4:+49099148 MS.gene38977:CDS
TATGCGGGTGTAGAAGATGG+TGG 0.728879 8.4:+49099143 MS.gene38977:CDS
ACGTACCATCAACAATCACG+TGG 0.741961 8.4:-49098416 None:intergenic
GAGGAATTGAAGATCCCATG+GGG 0.782196 8.4:-49098010 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAAAATCACTATGAAAAT+TGG + chr8.4:49098958-49098977 MS.gene38977:CDS 20.0%
! AAAATTCAAAGCAAACTTAG+AGG + chr8.4:49098656-49098675 MS.gene38977:CDS 25.0%
! AACAGTGATTCATTAGTAAT+TGG + chr8.4:49099017-49099036 MS.gene38977:CDS 25.0%
! AATGAGGATAATTATGAAGA+AGG + chr8.4:49098576-49098595 MS.gene38977:CDS 25.0%
! AATGGAGTTAAAAACTATGA+AGG + chr8.4:49098321-49098340 MS.gene38977:CDS 25.0%
! AATTACTAATGAATCACTGT+TGG - chr8.4:49099018-49099037 None:intergenic 25.0%
! ATTGTGAAAGGAAATCTAAT+GGG - chr8.4:49098546-49098565 None:intergenic 25.0%
! TATTGTGAAAGGAAATCTAA+TGG - chr8.4:49098547-49098566 None:intergenic 25.0%
! TTGTTCTATCTAATTCGAAA+GGG - chr8.4:49098074-49098093 None:intergenic 25.0%
! TTTGTTCTATCTAATTCGAA+AGG - chr8.4:49098075-49098094 None:intergenic 25.0%
!! CATTTAAAGCAAAAAATGAG+TGG - chr8.4:49098305-49098324 None:intergenic 25.0%
!!! AACACTAATGTTTTTTGCTA+CGG - chr8.4:49098244-49098263 None:intergenic 25.0%
!!! CACTCATTTTTTGCTTTAAA+TGG + chr8.4:49098303-49098322 MS.gene38977:CDS 25.0%
!!! GAGAGTATTTCATTTCAAAA+AGG + chr8.4:49098617-49098636 MS.gene38977:CDS 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr8.4:49099056-49099075 MS.gene38977:CDS 25.0%
!!! TTATGGTAATGATTTTGGTT+GGG + chr8.4:49099061-49099080 MS.gene38977:CDS 25.0%
!!! TTTATGGTAATGATTTTGGT+TGG + chr8.4:49099060-49099079 MS.gene38977:CDS 25.0%
AGTGATTCATTAGTAATTGG+TGG + chr8.4:49099020-49099039 MS.gene38977:CDS 30.0%
TAGAAATGATCCTGAGTTTA+TGG + chr8.4:49099211-49099230 MS.gene38977:CDS 30.0%
TAGAAGAAAGAGAGTCTTTA+AGG - chr8.4:49098110-49098129 None:intergenic 30.0%
TGGAAAGATTTATGTGTATG+CGG + chr8.4:49099127-49099146 MS.gene38977:CDS 30.0%
TTACCATAAACATCAAACCA+AGG - chr8.4:49099050-49099069 None:intergenic 30.0%
TTGAAATGAAATACTCTCTC+TGG - chr8.4:49098615-49098634 None:intergenic 30.0%
TTGGCATTTCATCAATTCAT+GGG + chr8.4:49098443-49098462 MS.gene38977:CDS 30.0%
TTTGGCATTTCATCAATTCA+TGG + chr8.4:49098442-49098461 MS.gene38977:CDS 30.0%
! ATGTCTTCCTTGTGAAATTT+TGG + chr8.4:49099181-49099200 MS.gene38977:CDS 30.0%
!!! CTTCTACCCTTGATATTTTT+TGG + chr8.4:49098124-49098143 MS.gene38977:CDS 30.0%
!!! TATGGTAATGATTTTGGTTG+GGG + chr8.4:49099062-49099081 MS.gene38977:CDS 30.0%
!!! TTTGATCGTTGGTTTTCAAA+AGG + chr8.4:49098510-49098529 MS.gene38977:CDS 30.0%
AAAAATGAGTGGTGAATAGG+AGG - chr8.4:49098294-49098313 None:intergenic 35.0%
AACTTCCAACATTTGATCGT+TGG + chr8.4:49098499-49098518 MS.gene38977:CDS 35.0%
ACGATCAAATGTTGGAAGTT+TGG - chr8.4:49098499-49098518 None:intergenic 35.0%
ATTGCCTCCAAAATTTCACA+AGG - chr8.4:49099191-49099210 None:intergenic 35.0%
CAAGTAACAGAGTTAATCGA+CGG + chr8.4:49098369-49098388 MS.gene38977:CDS 35.0%
CATTTGGATTCAACCAAAAG+GGG + chr8.4:49098034-49098053 MS.gene38977:CDS 35.0%
CCATTTGGATTCAACCAAAA+GGG + chr8.4:49098033-49098052 MS.gene38977:CDS 35.0%
GAAAACCAACGATCAAATGT+TGG - chr8.4:49098507-49098526 None:intergenic 35.0%
GAAGGGCCAAAAAATATCAA+GGG - chr8.4:49098133-49098152 None:intergenic 35.0%
GCAAAAAATGAGTGGTGAAT+AGG - chr8.4:49098297-49098316 None:intergenic 35.0%
GCATTACCAAAATAATCCTC+AGG - chr8.4:49098825-49098844 None:intergenic 35.0%
GGAAAGATTTATGTGTATGC+GGG + chr8.4:49099128-49099147 MS.gene38977:CDS 35.0%
TGAAGGGCCAAAAAATATCA+AGG - chr8.4:49098134-49098153 None:intergenic 35.0%
TGAATAGGAGGATGATAAGT+GGG - chr8.4:49098282-49098301 None:intergenic 35.0%
TGGATATTGGAATTAGACCA+AGG + chr8.4:49098784-49098803 MS.gene38977:CDS 35.0%
TTAATCGACGGCATCTTTAT+TGG + chr8.4:49098381-49098400 MS.gene38977:CDS 35.0%
TTCAAAGCAAACTTAGAGGT+TGG + chr8.4:49098660-49098679 MS.gene38977:CDS 35.0%
TTCTATCTAATTCGAAAGGG+TGG - chr8.4:49098071-49098090 None:intergenic 35.0%
TTTCGTGACTCTATTGTGAA+AGG - chr8.4:49098558-49098577 None:intergenic 35.0%
! AAGCAATTTTCGCTCACATT+TGG + chr8.4:49098706-49098725 MS.gene38977:CDS 35.0%
! ATAAGTGGGTTTAACGATGT+CGG - chr8.4:49098268-49098287 None:intergenic 35.0%
! TCTCCTTGGTTTGATGTTTA+TGG + chr8.4:49099044-49099063 MS.gene38977:CDS 35.0%
! TCTTCCTTGTGAAATTTTGG+AGG + chr8.4:49099184-49099203 MS.gene38977:CDS 35.0%
! TGTGTTGTTAGCATTAAAGC+CGG + chr8.4:49098855-49098874 MS.gene38977:CDS 35.0%
!! AAAGAGAGTCTTTAAGGTGT+TGG - chr8.4:49098104-49098123 None:intergenic 35.0%
!! CCTTTTGGTTGAATCCAAAT+GGG - chr8.4:49098035-49098054 None:intergenic 35.0%
!!! ATGGTAATGATTTTGGTTGG+GGG + chr8.4:49099063-49099082 MS.gene38977:CDS 35.0%
ACGAATTTCACTTCTGCTTG+TGG - chr8.4:49098762-49098781 None:intergenic 40.0%
ATACAATCCATCTAACCCCA+TGG + chr8.4:49097995-49098014 MS.gene38977:CDS 40.0%
ATACCAGAGGTGACAAAACT+CGG - chr8.4:49098993-49099012 None:intergenic 40.0%
CACAAGGAAGACATACTTCA+AGG - chr8.4:49099175-49099194 None:intergenic 40.0%
CACAATAGAGTCACGAAATG+AGG + chr8.4:49098560-49098579 MS.gene38977:CDS 40.0%
CATGTATTGTAGTTGTGGAG+AGG - chr8.4:49097942-49097961 None:intergenic 40.0%
CCAAAATAATCCTCAGGCAA+CGG - chr8.4:49098819-49098838 None:intergenic 40.0%
CCATCTTGTGCAACAACAAA+GGG + chr8.4:49098202-49098221 MS.gene38977:CDS 40.0%
CCCATTTGGATTCAACCAAA+AGG + chr8.4:49098032-49098051 MS.gene38977:CDS 40.0%
CGAATTTCACTTCTGCTTGT+GGG - chr8.4:49098761-49098780 None:intergenic 40.0%
GACACAACATCCATAAACTC+AGG - chr8.4:49099224-49099243 None:intergenic 40.0%
GATCTTCAATTCCTCCCATT+TGG + chr8.4:49098018-49098037 MS.gene38977:CDS 40.0%
GTGAAATTCGTGCTGGATAT+TGG + chr8.4:49098771-49098790 MS.gene38977:CDS 40.0%
GTGAATAGGAGGATGATAAG+TGG - chr8.4:49098283-49098302 None:intergenic 40.0%
GTTAACCACGTGATTGTTGA+TGG + chr8.4:49098411-49098430 MS.gene38977:CDS 40.0%
GTTACTTGGACTGCAAGTAA+CGG - chr8.4:49098357-49098376 None:intergenic 40.0%
TACAATCCATCTAACCCCAT+GGG + chr8.4:49097996-49098015 MS.gene38977:CDS 40.0%
TCCATCTTGTGCAACAACAA+AGG + chr8.4:49098201-49098220 MS.gene38977:CDS 40.0%
TGCTGCATGTATTGTAGTTG+TGG - chr8.4:49097947-49097966 None:intergenic 40.0%
TTTGGTTGAATCCAAATGGG+AGG - chr8.4:49098032-49098051 None:intergenic 40.0%
! CGTCGATTAACTCTGTTACT+TGG - chr8.4:49098371-49098390 None:intergenic 40.0%
! TAGTAATTGGTGGTTCTCCT+TGG + chr8.4:49099030-49099049 MS.gene38977:CDS 40.0%
!! CCCTTTTGGTTGAATCCAAA+TGG - chr8.4:49098036-49098055 None:intergenic 40.0%
!!! GATATTTTTTGGCCCTTCAC+AGG + chr8.4:49098135-49098154 MS.gene38977:CDS 40.0%
!!! TTGTTGATGGTACGTCGTTT+TGG + chr8.4:49098424-49098443 MS.gene38977:CDS 40.0%
AAATAATCCTCAGGCAACGG+AGG - chr8.4:49098816-49098835 None:intergenic 45.0%
ACCATCTTCCAACAAGTCAC+CGG - chr8.4:49098877-49098896 None:intergenic 45.0%
ACGTACCATCAACAATCACG+TGG - chr8.4:49098419-49098438 None:intergenic 45.0%
AGCAGAAGTGAAATTCGTGC+TGG + chr8.4:49098764-49098783 MS.gene38977:CDS 45.0%
AGTTGTGGAGAGGACTTTGA+TGG - chr8.4:49097932-49097951 None:intergenic 45.0%
ATCTTGAGACGACCTGTGAA+GGG - chr8.4:49098150-49098169 None:intergenic 45.0%
CAACTTCTCATCTGTGTGCA+AGG - chr8.4:49098940-49098959 None:intergenic 45.0%
GAGGAATTGAAGATCCCATG+GGG - chr8.4:49098013-49098032 None:intergenic 45.0%
GGAGGAATTGAAGATCCCAT+GGG - chr8.4:49098014-49098033 None:intergenic 45.0%
GTGAGCGAAAATTGCTTGCA+AGG - chr8.4:49098703-49098722 None:intergenic 45.0%
TATCTTGAGACGACCTGTGA+AGG - chr8.4:49098151-49098170 None:intergenic 45.0%
! CATTAAAGCCGGTGACTTGT+TGG + chr8.4:49098866-49098885 MS.gene38977:CDS 45.0%
! CCGTTGCCTGAGGATTATTT+TGG + chr8.4:49098816-49098835 MS.gene38977:CDS 45.0%
! GGTGATAAAGAAGCCCCTTT+TGG - chr8.4:49098050-49098069 None:intergenic 45.0%
! TCATGGGCTGAAATCTCGAA+AGG + chr8.4:49098459-49098478 MS.gene38977:CDS 45.0%
!! CCCTTTGTTGTTGCACAAGA+TGG - chr8.4:49098205-49098224 None:intergenic 45.0%
!! GAGTTTTGTCACCTCTGGTA+TGG + chr8.4:49098992-49099011 MS.gene38977:CDS 45.0%
!! GTGACTTGTTGGAAGATGGT+GGG + chr8.4:49098877-49098896 MS.gene38977:CDS 45.0%
ATCACTGTTGGCCATACCAG+AGG - chr8.4:49099006-49099025 None:intergenic 50.0%
GAAGATCCCATGGGGTTAGA+TGG - chr8.4:49098005-49098024 None:intergenic 50.0%
GCTACGGCATGAACAAAGAG+TGG - chr8.4:49098228-49098247 None:intergenic 50.0%
GGGAGGAATTGAAGATCCCA+TGG - chr8.4:49098015-49098034 None:intergenic 50.0%
GTGTATGCGGGTGTAGAAGA+TGG + chr8.4:49099140-49099159 MS.gene38977:CDS 50.0%
TATGCGGGTGTAGAAGATGG+TGG + chr8.4:49099143-49099162 MS.gene38977:CDS 50.0%
TTTGGTTGGGGGAAACCTGT+GGG + chr8.4:49099074-49099093 MS.gene38977:CDS 50.0%
! GGGGGTGCGAGTAAAAGAAA+TGG + chr8.4:49099107-49099126 MS.gene38977:CDS 50.0%
!! ACACCGAGTTTTGTCACCTC+TGG + chr8.4:49098987-49099006 MS.gene38977:CDS 50.0%
!! GAAGATGGTGGGCTTGGTAA+GGG + chr8.4:49098888-49098907 MS.gene38977:CDS 50.0%
!! GGTGACTTGTTGGAAGATGG+TGG + chr8.4:49098876-49098895 MS.gene38977:CDS 50.0%
!! TTGTTGGAAGATGGTGGGCT+TGG + chr8.4:49098882-49098901 MS.gene38977:CDS 50.0%
!! TTTTGGTTGGGGGAAACCTG+TGG + chr8.4:49099073-49099092 MS.gene38977:CDS 50.0%
AAACCTGTGGGGGTTCGAAG+TGG + chr8.4:49099086-49099105 MS.gene38977:CDS 55.0%
AACCTGTGGGGGTTCGAAGT+GGG + chr8.4:49099087-49099106 MS.gene38977:CDS 55.0%
GGCAACGGAGGAATCAACCT+TGG - chr8.4:49098804-49098823 None:intergenic 55.0%
TTGGTTGGGGGAAACCTGTG+GGG + chr8.4:49099075-49099094 MS.gene38977:CDS 55.0%
! GGGTGTAGAAGATGGTGGCA+TGG + chr8.4:49099148-49099167 MS.gene38977:CDS 55.0%
! GTTGATTCCTCCGTTGCCTG+AGG + chr8.4:49098806-49098825 MS.gene38977:CDS 55.0%
! TGGGCTTGGTAAGGGCTCTT+TGG + chr8.4:49098896-49098915 MS.gene38977:CDS 55.0%
!! GCCGGTGACTTGTTGGAAGA+TGG + chr8.4:49098873-49098892 MS.gene38977:CDS 55.0%
!! GGAAGATGGTGGGCTTGGTA+AGG + chr8.4:49098887-49098906 MS.gene38977:CDS 55.0%
ACCTGTGGGGGTTCGAAGTG+GGG + chr8.4:49099088-49099107 MS.gene38977:CDS 60.0%
TGGTTGGGGGAAACCTGTGG+GGG + chr8.4:49099076-49099095 MS.gene38977:CDS 60.0%
CCTGTGGGGGTTCGAAGTGG+GGG + chr8.4:49099089-49099108 MS.gene38977:CDS 65.0%
CCCCCACTTCGAACCCCCAC+AGG - chr8.4:49099092-49099111 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 49097922 49099250 49097922 ID=MS.gene38977
chr8.4 mRNA 49097922 49099250 49097922 ID=MS.gene38977.t1;Parent=MS.gene38977
chr8.4 exon 49097922 49099250 49097922 ID=MS.gene38977.t1.exon1;Parent=MS.gene38977.t1
chr8.4 CDS 49097922 49099250 49097922 ID=cds.MS.gene38977.t1;Parent=MS.gene38977.t1
Gene Sequence

>MS.gene38977

ATGAGTTCCATCAAAGTCCTCTCCACAACTACAATACATGCAGCAAATCATAATCTTTCTGACTCAACTTATCATACAATCCATCTAACCCCATGGGATCTTCAATTCCTCCCATTTGGATTCAACCAAAAGGGGCTTCTTTATCACCACCCTTTCGAATTAGATAGAACAAATCAAATCCAACACCTTAAAGACTCTCTTTCTTCTACCCTTGATATTTTTTGGCCCTTCACAGGTCGTCTCAAGATAACAGAACACGAAGCTAATACTGTCTCGTGTTCCATCTTGTGCAACAACAAAGGGCCACTCTTTGTTCATGCCGTAGCAAAAAACATTAGTGTTGCCGACATCGTTAAACCCACTTATCATCCTCCTATTCACCACTCATTTTTTGCTTTAAATGGAGTTAAAAACTATGAAGGAACATCACAACCGTTACTTGCAGTCCAAGTAACAGAGTTAATCGACGGCATCTTTATTGGCTTCACAGTTAACCACGTGATTGTTGATGGTACGTCGTTTTGGCATTTCATCAATTCATGGGCTGAAATCTCGAAAGGTTGTCTTAAAATATCCAAACTTCCAACATTTGATCGTTGGTTTTCAAAAGGTATTGAATGTCCCATTAGATTTCCTTTCACAATAGAGTCACGAAATGAGGATAATTATGAAGAAGGAAAAATCAACTTTCCAGAGAGAGTATTTCATTTCAAAAAGGAGAGTATCGCAAAACTAAAATTCAAAGCAAACTTAGAGGTTGGTACAAAAACTATATCTTCCTTGCAAGCAATTTTCGCTCACATTTGGCGCTCTATTATACGTTGCAAAAAACTTGACCCACAAGCAGAAGTGAAATTCGTGCTGGATATTGGAATTAGACCAAGGTTGATTCCTCCGTTGCCTGAGGATTATTTTGGTAATGCTGTGATAGATTGTGTTGTTAGCATTAAAGCCGGTGACTTGTTGGAAGATGGTGGGCTTGGTAAGGGCTCTTTGGAGATGAACAAGACGATTGCCTTGCACACAGATGAGAAGTTGAAAAATCACTATGAAAATTGGTTAATAACACCGAGTTTTGTCACCTCTGGTATGGCCAACAGTGATTCATTAGTAATTGGTGGTTCTCCTTGGTTTGATGTTTATGGTAATGATTTTGGTTGGGGGAAACCTGTGGGGGTTCGAAGTGGGGGTGCGAGTAAAAGAAATGGAAAGATTTATGTGTATGCGGGTGTAGAAGATGGTGGCATGGACCTTGAAGTATGTCTTCCTTGTGAAATTTTGGAGGCAATTAGAAATGATCCTGAGTTTATGGATGTTGTGTCCTACTAA
Protein sequence

>MS.gene38977.t1

MSSIKVLSTTTIHAANHNLSDSTYHTIHLTPWDLQFLPFGFNQKGLLYHHPFELDRTNQIQHLKDSLSSTLDIFWPFTGRLKITEHEANTVSCSILCNNKGPLFVHAVAKNISVADIVKPTYHPPIHHSFFALNGVKNYEGTSQPLLAVQVTELIDGIFIGFTVNHVIVDGTSFWHFINSWAEISKGCLKISKLPTFDRWFSKGIECPIRFPFTIESRNEDNYEEGKINFPERVFHFKKESIAKLKFKANLEVGTKTISSLQAIFAHIWRSIIRCKKLDPQAEVKFVLDIGIRPRLIPPLPEDYFGNAVIDCVVSIKAGDLLEDGGLGKGSLEMNKTIALHTDEKLKNHYENWLITPSFVTSGMANSDSLVIGGSPWFDVYGNDFGWGKPVGVRSGGASKRNGKIYVYAGVEDGGMDLEVCLPCEILEAIRNDPEFMDVVSY