AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28251

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28251.t1 MTR_3g026310 88.419 449 52 0 1 449 1 449 0.0 822
MS.gene28251.t1 MTR_3g088420 72.606 449 108 3 1 449 1 434 0.0 671
MS.gene28251.t1 MTR_4g022825 66.518 448 142 3 1 448 1 440 0.0 609
MS.gene28251.t1 MTR_4g022800 66.147 449 146 2 1 449 1 443 0.0 605
MS.gene28251.t1 MTR_4g022720 66.147 449 139 3 1 449 1 436 0.0 597
MS.gene28251.t1 MTR_4g022670 64.811 449 151 2 1 449 1 442 0.0 593
MS.gene28251.t1 MTR_7g026860 64.588 449 149 2 1 449 1 439 0.0 590
MS.gene28251.t1 MTR_4g022760 59.020 449 133 2 1 449 1 398 0.0 523
MS.gene28251.t1 MTR_8g041770 56.522 460 171 6 1 449 1 442 9.42e-179 507
MS.gene28251.t1 MTR_0190s0020 55.773 459 175 7 1 448 1 442 3.40e-173 493
MS.gene28251.t1 MTR_4g094842 55.411 462 170 11 1 444 1 444 9.04e-168 479
MS.gene28251.t1 MTR_4g094835 51.515 462 186 10 1 444 1 442 1.38e-158 456
MS.gene28251.t1 MTR_4g094828 51.183 465 195 11 1 448 1 450 6.91e-151 436
MS.gene28251.t1 MTR_8g079710 50.649 462 189 12 1 444 1 441 6.54e-148 429
MS.gene28251.t1 MTR_6g016815 52.212 452 129 9 1 449 15 382 1.11e-142 413
MS.gene28251.t1 MTR_4g022290 49.251 467 190 13 1 445 1 442 3.53e-141 411
MS.gene28251.t1 MTR_4g094838 48.060 464 182 10 4 444 1 428 2.14e-139 407
MS.gene28251.t1 MTR_4g094832 52.051 390 153 7 69 444 57 426 7.21e-133 390
MS.gene28251.t1 MTR_8g040760 64.977 217 69 3 1 211 1 216 4.71e-93 280
MS.gene28251.t1 MTR_3g101900 40.961 437 228 12 24 448 30 448 1.44e-91 285
MS.gene28251.t1 MTR_3g101930 39.101 445 230 11 24 447 30 454 2.60e-88 276
MS.gene28251.t1 MTR_3g102020 37.923 443 237 11 24 448 29 451 3.88e-85 268
MS.gene28251.t1 MTR_3g101970 37.727 440 226 11 24 447 29 436 2.43e-84 266
MS.gene28251.t1 MTR_3g102010 37.783 442 238 12 24 448 30 451 1.57e-80 256
MS.gene28251.t1 MTR_3g101960 37.701 435 238 11 24 443 29 445 3.75e-80 255
MS.gene28251.t1 MTR_7g026850 68.553 159 50 0 29 187 12 170 8.38e-76 235
MS.gene28251.t1 MTR_8g040780 55.760 217 90 3 228 440 10 224 8.67e-76 237
MS.gene28251.t1 MTR_4g015180 34.310 478 260 15 17 449 20 488 1.32e-65 218
MS.gene28251.t1 MTR_5g081960 32.558 473 262 13 22 448 25 486 2.98e-64 215
MS.gene28251.t1 MTR_3g070170 30.932 472 290 13 2 448 10 470 6.72e-62 208
MS.gene28251.t1 MTR_7g026770 55.897 195 53 2 141 335 132 293 7.51e-58 192
MS.gene28251.t1 MTR_4g094825 56.287 167 66 4 16 176 83 248 1.43e-55 189
MS.gene28251.t1 MTR_4g094825 48.101 158 67 3 289 444 243 387 7.32e-39 145
MS.gene28251.t1 MTR_6g034495 64.800 125 41 1 64 185 43 167 3.97e-50 168
MS.gene28251.t1 MTR_3g102030 35.202 321 161 9 87 404 44 320 1.30e-49 172
MS.gene28251.t1 MTR_6g045553 28.970 466 298 10 4 444 14 471 3.01e-48 172
MS.gene28251.t1 MTR_6g034485 57.971 138 45 3 188 325 1 125 4.53e-46 156
MS.gene28251.t1 MTR_7g026830 70.588 85 25 0 82 166 14 98 1.75e-35 127
MS.gene28251.t1 MTR_4g007540 25.877 456 277 15 18 449 14 432 2.94e-32 127
MS.gene28251.t1 MTR_8g075610 25.871 402 244 15 55 437 47 413 1.16e-30 122
MS.gene28251.t1 MTR_3g026950 27.160 405 233 16 30 400 28 404 1.89e-28 117
MS.gene28251.t1 MTR_7g026800 41.667 192 69 7 211 395 18 173 2.68e-28 110
MS.gene28251.t1 MTR_8g096530 28.502 414 238 15 55 441 59 441 3.72e-28 116
MS.gene28251.t1 MTR_3g049150 27.972 429 249 16 1 399 1 399 1.08e-26 112
MS.gene28251.t1 MTR_4g022780 39.896 193 77 7 200 389 7 163 1.58e-26 105
MS.gene28251.t1 MTR_2g105330 25.693 397 240 13 55 430 56 418 1.62e-26 111
MS.gene28251.t1 MTR_4g097090 25.481 416 264 15 55 447 65 457 3.05e-26 110
MS.gene28251.t1 MTR_3g098980 28.125 384 236 13 55 429 66 418 4.95e-26 110
MS.gene28251.t1 MTR_2g064495 26.250 400 234 16 55 430 56 418 8.45e-26 109
MS.gene28251.t1 MTR_7g026780 46.903 113 34 1 337 449 26 112 2.02e-24 97.8
MS.gene28251.t1 MTR_7g014250 27.635 427 254 15 14 406 11 416 9.87e-24 103
MS.gene28251.t1 MTR_7g014330 26.914 431 261 17 6 406 10 416 7.21e-23 100
MS.gene28251.t1 MTR_7g014230 26.852 432 260 18 6 406 12 418 3.59e-21 95.9
MS.gene28251.t1 MTR_3g087640 25.386 453 290 13 17 435 24 462 1.11e-20 94.7
MS.gene28251.t1 MTR_6g015815 23.696 460 282 18 15 437 25 452 1.12e-20 94.7
MS.gene28251.t1 MTR_7g027175 25.000 396 260 11 49 435 62 429 1.21e-20 94.4
MS.gene28251.t1 MTR_2g089670 26.055 403 259 12 21 399 42 429 4.22e-20 92.8
MS.gene28251.t1 MTR_4g127650 23.644 461 287 18 21 447 28 457 4.32e-20 92.8
MS.gene28251.t1 MTR_4g013050 24.728 368 202 12 53 400 51 363 5.64e-20 92.0
MS.gene28251.t1 MTR_2g089765 23.094 446 283 13 24 429 39 464 1.19e-19 91.7
MS.gene28251.t1 MTR_6g015320 25.240 313 208 11 138 435 143 444 1.44e-19 91.3
MS.gene28251.t1 MTR_7g014160 24.059 478 282 17 3 429 8 455 2.07e-19 90.9
MS.gene28251.t1 MTR_6g015315 27.329 322 197 13 138 438 140 445 2.45e-19 90.5
MS.gene28251.t1 MTR_5g026320 26.329 414 247 18 52 443 71 448 3.76e-19 90.1
MS.gene28251.t1 MTR_8g012750 26.222 450 264 19 3 406 2 429 4.08e-19 90.1
MS.gene28251.t1 MTR_1g081660 25.187 401 241 16 24 400 31 396 6.98e-19 89.0
MS.gene28251.t1 MTR_3g028210 23.060 464 298 16 4 430 8 449 2.47e-18 87.4
MS.gene28251.t1 MTR_3g086020 26.121 379 233 13 55 429 61 396 1.16e-17 85.1
MS.gene28251.t1 MTR_2g089650 23.438 448 299 15 7 425 11 443 4.18e-17 83.6
MS.gene28251.t1 MTR_1g053315 24.205 409 269 17 55 444 78 464 7.11e-17 83.2
MS.gene28251.t1 MTR_3g049140 28.342 187 106 6 233 400 20 197 1.25e-16 79.7
MS.gene28251.t1 MTR_7g114520 23.691 401 248 16 25 400 25 392 1.31e-16 82.0
MS.gene28251.t1 MTR_2g089735 24.286 420 268 14 38 425 40 441 1.55e-16 82.0
MS.gene28251.t1 MTR_6g015830 24.401 459 289 17 10 437 21 452 1.58e-16 82.0
MS.gene28251.t1 MTR_7g026870 60.000 55 22 0 302 356 97 151 2.06e-16 76.6
MS.gene28251.t1 MTR_7g014360 27.857 280 168 6 138 406 90 346 4.60e-16 80.1
MS.gene28251.t1 MTR_5g090810 25.500 400 244 17 32 400 36 412 1.10e-15 79.3
MS.gene28251.t1 MTR_7g026880 63.462 52 19 0 397 448 2 53 1.65e-15 70.9
MS.gene28251.t1 MTR_6g015850 24.406 463 281 19 10 437 21 449 2.00e-15 78.6
MS.gene28251.t1 MTR_7g114550 22.870 446 281 19 25 440 24 436 2.52e-15 78.2
MS.gene28251.t1 MTR_5g084870 23.131 428 286 13 4 421 3 397 2.98e-15 77.8
MS.gene28251.t1 MTR_6g053810 26.970 330 200 13 49 364 65 367 5.36e-15 77.4
MS.gene28251.t1 MTR_5g090830 25.750 400 243 17 32 400 36 412 5.77e-15 77.0
MS.gene28251.t1 MTR_4g055170 25.704 284 185 8 55 330 69 334 1.17e-14 76.3
MS.gene28251.t1 MTR_3g112180 23.869 398 268 12 48 435 54 426 1.84e-14 75.5
MS.gene28251.t1 MTR_7g014200 23.391 466 297 13 2 429 7 450 3.69e-14 74.7
MS.gene28251.t1 MTR_3g071320 23.587 407 262 12 54 444 59 432 5.23e-14 73.9
MS.gene28251.t1 MTR_3g465980 25.714 315 205 12 55 364 68 358 6.80e-14 73.9
MS.gene28251.t1 MTR_7g086000 25.432 405 259 17 58 437 63 449 9.32e-14 73.6
MS.gene28251.t1 MTR_7g056087 22.051 390 279 10 54 435 57 429 2.74e-13 72.0
MS.gene28251.t1 MTR_2g015430 23.796 353 234 12 58 402 61 386 3.10e-13 71.6
MS.gene28251.t1 MTR_7g014610 23.918 485 270 21 22 449 27 469 3.25e-13 71.6
MS.gene28251.t1 MTR_1g094790 26.136 440 265 18 29 446 38 439 5.16e-13 70.9
MS.gene28251.t1 MTR_0502s0010 30.189 212 108 8 134 330 39 225 1.24e-12 67.8
MS.gene28251.t1 MTR_3g465910 27.164 335 185 17 50 364 63 358 3.76e-12 68.6
MS.gene28251.t1 MTR_7g014310 27.972 143 97 2 264 406 147 283 7.40e-12 66.6
MS.gene28251.t1 MTR_7g014620 24.654 434 265 12 24 429 15 414 1.11e-11 67.0
MS.gene28251.t1 MTR_3g465990 23.585 318 208 12 55 364 68 358 1.36e-11 66.6
MS.gene28251.t1 MTR_7g014670 28.966 145 94 3 287 429 104 241 2.94e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28251.t1 AT5G67150 41.037 463 239 12 1 447 1 445 4.80e-104 317
MS.gene28251.t1 AT3G50280 40.044 457 245 10 1 445 1 440 7.46e-103 313
MS.gene28251.t1 AT3G50300 42.630 441 224 12 1 429 1 424 2.13e-102 313
MS.gene28251.t1 AT5G67160 39.911 451 239 12 3 448 9 432 1.22e-97 300
MS.gene28251.t1 AT3G50270 37.908 459 255 12 4 448 6 448 2.96e-92 286
MS.gene28251.t1 AT5G38130 38.409 440 242 10 1 429 21 442 2.78e-89 279
MS.gene28251.t1 AT5G42830 37.862 449 244 12 15 448 21 449 9.68e-87 272
MS.gene28251.t1 AT5G07860 36.069 463 259 11 2 447 6 448 3.05e-79 253
MS.gene28251.t1 AT5G07870 36.383 470 264 13 2 449 6 462 4.66e-76 245
MS.gene28251.t1 AT3G50280 41.284 327 169 6 125 445 1 310 3.59e-74 235
MS.gene28251.t1 AT2G39980 32.649 487 247 13 17 449 22 481 1.35e-69 229
MS.gene28251.t1 AT5G07850 34.649 456 254 14 15 449 22 454 5.56e-68 224
MS.gene28251.t1 AT5G01210 33.613 476 246 13 17 448 20 469 5.71e-65 216
MS.gene28251.t1 AT3G50290 41.259 286 144 6 119 397 1 269 2.72e-59 196
MS.gene28251.t1 AT5G23940 30.753 465 279 16 4 444 3 448 2.31e-53 186
MS.gene28251.t1 AT2G19070 25.727 447 289 16 27 445 19 450 1.14e-25 109
MS.gene28251.t1 AT5G57840 28.182 440 282 16 4 429 3 422 1.28e-25 108
MS.gene28251.t1 AT5G48930 26.786 392 242 13 27 400 23 387 2.54e-25 108
MS.gene28251.t1 AT3G48720 25.428 409 262 12 42 436 44 423 9.87e-24 103
MS.gene28251.t1 AT5G63560 27.273 385 239 16 55 430 61 413 2.17e-21 96.3
MS.gene28251.t1 AT3G29590 25.702 463 293 16 2 439 6 442 4.16e-21 95.9
MS.gene28251.t1 AT2G40230 27.824 363 223 13 54 405 58 392 3.04e-20 93.2
MS.gene28251.t1 AT1G28680 27.528 356 226 15 58 407 65 394 3.57e-20 93.2
MS.gene28251.t1 AT3G26040 25.962 416 253 15 49 448 53 429 5.72e-20 92.4
MS.gene28251.t1 AT3G29670 22.444 450 306 14 1 429 6 433 1.58e-18 88.2
MS.gene28251.t1 AT5G16410 24.138 377 255 11 32 400 66 419 1.13e-17 85.5
MS.gene28251.t1 AT3G29635 24.942 433 279 14 22 429 27 438 1.68e-17 85.1
MS.gene28251.t1 AT5G07080 26.690 281 179 10 55 327 70 331 8.94e-17 82.8
MS.gene28251.t1 AT1G28680 27.907 301 194 13 58 354 65 346 1.18e-16 82.0
MS.gene28251.t1 AT1G03390 25.735 408 262 16 42 430 59 444 4.04e-16 80.9
MS.gene28251.t1 AT5G23970 24.877 406 269 15 49 448 46 421 1.68e-15 78.6
MS.gene28251.t1 AT1G78990 25.203 369 241 13 43 400 52 396 7.99e-15 76.6
MS.gene28251.t1 AT5G61160 22.030 404 279 14 22 406 23 409 1.97e-14 75.5
MS.gene28251.t1 AT3G29680 23.250 400 260 13 24 400 25 400 2.67e-14 75.1
MS.gene28251.t1 AT1G24430 24.934 377 234 15 54 421 61 397 2.83e-14 75.1
MS.gene28251.t1 AT5G39050 25.698 358 205 10 120 435 130 468 1.25e-13 73.2
MS.gene28251.t1 AT1G24420 24.928 349 209 17 65 402 75 381 2.70e-13 72.0
MS.gene28251.t1 AT1G24420 24.928 349 209 17 65 402 75 381 2.70e-13 72.0
MS.gene28251.t1 AT3G47170 25.902 305 181 12 44 327 64 344 3.81e-11 65.5

Find 95 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGACATGGTGTTGTCTTTA+TGG 0.155383 3.3:+18194090 None:intergenic
TCCCCGCGGTTTGATGTTTA+TGG 0.196862 3.3:-18193171 MS.gene28251:CDS
GTGTCTTCCTCACAAGATTT+TGG 0.229479 3.3:-18193040 MS.gene28251:CDS
CCGTTGCCGAAAGATTATTT+TGG 0.284850 3.3:-18193408 MS.gene28251:CDS
GATGTTTATGGCAATAATTT+TGG 0.301289 3.3:-18193159 MS.gene28251:CDS
GCCTGCAAGTGGTGGGAAAA+AGG 0.319207 3.3:+18194130 None:intergenic
GGTGATAAAGGAGACCCTTT+TGG 0.329078 3.3:+18194210 None:intergenic
TTAGTTGACGGCATCTTTAT+TGG 0.332453 3.3:-18193885 MS.gene28251:CDS
ACGTTTAAGTGTTGAGAGTT+TGG 0.343241 3.3:+18193764 None:intergenic
TTTAACCATCTGGTTGTTGA+TGG 0.349436 3.3:-18193855 MS.gene28251:CDS
AAATGCTGTTGTAGGTTCTA+TGG 0.351981 3.3:-18193385 MS.gene28251:CDS
TTGAGTGCAAAGAAATCATT+TGG 0.352198 3.3:+18193298 None:intergenic
GGACTGGTTGTTGTATTTGC+TGG 0.355262 3.3:-18193093 MS.gene28251:CDS
ACTCTAGAGGATGGTGATAA+AGG 0.360277 3.3:+18194198 None:intergenic
GCATTTCCAAAATAATCTTT+CGG 0.388444 3.3:+18193402 None:intergenic
TGAAGAATGACTATGAGAAT+TGG 0.389153 3.3:-18193266 MS.gene28251:CDS
TTACCAAGTCTAAGGATGAT+TGG 0.394784 3.3:+18193234 None:intergenic
TGTTACTATTACCAAGTCTA+AGG 0.399364 3.3:+18193226 None:intergenic
CTATGGTTGCAATGAAAGTT+GGG 0.402541 3.3:-18193368 MS.gene28251:CDS
AATAGCCAACACACGATAAT+CGG 0.404923 3.3:+18193200 None:intergenic
CCTCTCTACAGATACAATAA+AGG 0.428765 3.3:-18194303 MS.gene28251:CDS
ATGGTATTTCATTAATTCGT+GGG 0.435786 3.3:-18193823 MS.gene28251:CDS
GTTGGTACCCATTCCTACAT+TGG 0.453150 3.3:+18193557 None:intergenic
TTCTTGCGGGTCTAATTGGT+CGG 0.459425 3.3:+18193479 None:intergenic
TCTATGGTTGCAATGAAAGT+TGG 0.459986 3.3:-18193369 MS.gene28251:CDS
ACTTGTTGAACCCACCCAAA+AGG 0.461769 3.3:-18194225 MS.gene28251:CDS
GAAGAATGACTATGAGAATT+GGG 0.462181 3.3:-18193265 MS.gene28251:CDS
TGGAATAGAAGAAGGTAGTA+TGG 0.465384 3.3:-18193073 MS.gene28251:CDS
GTTGGGGAGTTGTTGGAAGA+AGG 0.465812 3.3:-18193351 MS.gene28251:CDS
AAGCACTTTATGCACACCTT+TGG 0.465909 3.3:-18193518 MS.gene28251:CDS
CTTAATGAAGGGAAATCGTA+TGG 0.467781 3.3:+18193716 None:intergenic
AACTCTCAACACTTAAACGT+TGG 0.473857 3.3:-18193761 MS.gene28251:CDS
ATTGAGAATGGGTGGAATAT+AGG 0.483367 3.3:+18193983 None:intergenic
TTGAAAGCCAATGTAGGAAT+GGG 0.487514 3.3:-18193564 MS.gene28251:CDS
CCAAAATAATCTTTCGGCAA+CGG 0.489772 3.3:+18193408 None:intergenic
CGAATTTGGTTTGACTCTAG+AGG 0.489830 3.3:+18194185 None:intergenic
ATTGAAAGCCAATGTAGGAA+TGG 0.490950 3.3:-18193565 MS.gene28251:CDS
AATGAAAGTTGGGGAGTTGT+TGG 0.508990 3.3:-18193358 MS.gene28251:CDS
ATGGGACGTTGAATACCATC+TGG 0.510725 3.3:+18193735 None:intergenic
TATGGTCTATTATTTCTAGC+CGG 0.512204 3.3:+18194108 None:intergenic
GTATTTGCTGGAATAGAAGA+AGG 0.513089 3.3:-18193081 MS.gene28251:CDS
CTTGTTGAACCCACCCAAAA+GGG 0.515508 3.3:-18194224 MS.gene28251:CDS
TGTGAGGAAGACACACTTGA+AGG 0.517501 3.3:+18193049 None:intergenic
TCTAGCCGGCCTGCAAGTGG+TGG 0.518695 3.3:+18194122 None:intergenic
TGCCATAAACATCAAACCGC+GGG 0.519171 3.3:+18193169 None:intergenic
AGCGGATCACGGAGCACCAA+AGG 0.521379 3.3:+18193502 None:intergenic
CGCAGTGTAAATAATATAAG+TGG 0.523615 3.3:-18193114 MS.gene28251:CDS
TTGAAATTGAAAGCCAATGT+AGG 0.524132 3.3:-18193570 MS.gene28251:CDS
TTTACACTGCGAACCGCCAC+AGG 0.524757 3.3:+18193126 None:intergenic
TGTAAATAATATAAGTGGAC+TGG 0.528452 3.3:-18193109 MS.gene28251:CDS
TTGTATCTGTAGAGAGGACT+CGG 0.529270 3.3:+18194309 None:intergenic
GGTGTGCATAAAGTGCTTGA+AGG 0.530480 3.3:+18193523 None:intergenic
AAATGAAATAACCTCTCTGA+TGG 0.539126 3.3:+18193615 None:intergenic
TATGGTATTTCATTAATTCG+TGG 0.548533 3.3:-18193824 MS.gene28251:CDS
TTGTTGGAAGAAGGCGCACT+TGG 0.557654 3.3:-18193342 MS.gene28251:CDS
ATTTCTAGCCGGCCTGCAAG+TGG 0.561157 3.3:+18194119 None:intergenic
TCTTACTCCATGGGATCTCA+AGG 0.561820 3.3:-18194252 MS.gene28251:CDS
CATTGCCGATTATCGTGTGT+TGG 0.567779 3.3:-18193205 MS.gene28251:CDS
ACCAGCATTGTTGCATGTGA+CGG 0.568355 3.3:+18194064 None:intergenic
TACAAAAGAACAAATCTGAC+GGG 0.570323 3.3:-18193689 MS.gene28251:CDS
TAATTGGTCGGAGCGGATCA+CGG 0.571907 3.3:+18193491 None:intergenic
CTTAAACGTTGGTTTCCAGA+TGG 0.571951 3.3:-18193750 MS.gene28251:CDS
GTGACTTGCACCGCAAGCAA+TGG 0.572723 3.3:+18193912 None:intergenic
AATCCAATCATCCTTAGACT+TGG 0.582141 3.3:-18193237 MS.gene28251:CDS
TTTGGTTTGACTCTAGAGGA+TGG 0.583760 3.3:+18194189 None:intergenic
CAAGTCACAGAGTTAGTTGA+CGG 0.585682 3.3:-18193897 MS.gene28251:CDS
TCTGGTTGTTGATGGAACTT+CGG 0.586479 3.3:-18193847 MS.gene28251:CDS
GTGTGCATAAAGTGCTTGAA+GGG 0.587242 3.3:+18193524 None:intergenic
AGTAGAACCTTGAGATCCCA+TGG 0.591120 3.3:+18194245 None:intergenic
CCTTTATTGTATCTGTAGAG+AGG 0.600283 3.3:+18194303 None:intergenic
CTACAAAAGAACAAATCTGA+CGG 0.603675 3.3:-18193690 MS.gene28251:CDS
ACTTGTGTAGGTGACATACT+TGG 0.616854 3.3:-18194011 MS.gene28251:CDS
TCCGTCACATGCAACAATGC+TGG 0.617050 3.3:-18194065 MS.gene28251:CDS
ATCTGTAGAGAGGACTCGGA+TGG 0.618089 3.3:+18194313 None:intergenic
AAATAATCTTTCGGCAACGG+TGG 0.618582 3.3:+18193411 None:intergenic
TATGGTTGCAATGAAAGTTG+GGG 0.623366 3.3:-18193367 MS.gene28251:CDS
TTGTTGATGGAACTTCGGTA+TGG 0.625998 3.3:-18193842 MS.gene28251:CDS
CTAGCCGGCCTGCAAGTGGT+GGG 0.630047 3.3:+18194123 None:intergenic
AATGAAATAACCTCTCTGAT+GGG 0.633018 3.3:+18193616 None:intergenic
GAGGTTATTTCATTTCACAA+AGG 0.635344 3.3:-18193607 MS.gene28251:CDS
TGGATGGGGAAAACCTGTGG+CGG 0.653437 3.3:-18193139 MS.gene28251:CDS
TTAATGAAGGGAAATCGTAT+GGG 0.656726 3.3:+18193717 None:intergenic
AAGTTCCATCAACAACCAGA+TGG 0.663833 3.3:+18193850 None:intergenic
GCATGTGACGGAACACGACA+TGG 0.665285 3.3:+18194076 None:intergenic
ATTGCCTCCAAAATCTTGTG+AGG 0.672285 3.3:+18193033 None:intergenic
GCAATTGAGAACACTTGTGT+AGG 0.675928 3.3:-18194023 MS.gene28251:CDS
AAGTGTTCTCAATTGCAGCA+TGG 0.683875 3.3:+18194030 None:intergenic
TTGCCATAAACATCAAACCG+CGG 0.691006 3.3:+18193168 None:intergenic
GCGGGTCTAATTGGTCGGAG+CGG 0.694903 3.3:+18193484 None:intergenic
TCTCGATAGGAACTAGACAA+AGG 0.701206 3.3:-18193440 MS.gene28251:CDS
TCGTGGGCTGAAATCTCACG+TGG 0.711403 3.3:-18193807 MS.gene28251:CDS
GCCATAAACATCAAACCGCG+GGG 0.723500 3.3:+18193170 None:intergenic
TGGGCTGAAATCTCACGTGG+TGG 0.725908 3.3:-18193804 MS.gene28251:CDS
TACGTCACAACCATTGCTTG+CGG 0.749048 3.3:-18193922 MS.gene28251:CDS
TGGCTATTAGCAGTTCCCCG+CGG 0.755353 3.3:-18193185 MS.gene28251:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTCATCAGTTGAATT+TGG + chr3.3:18193038-18193057 None:intergenic 20.0%
! AAAAAATTGATCTTACTCCA+TGG - chr3.3:18193051-18193070 MS.gene28251:CDS 25.0%
! AAAAATTGATCTTACTCCAT+GGG - chr3.3:18193052-18193071 MS.gene28251:CDS 25.0%
! ATGGTATTTCATTAATTCGT+GGG - chr3.3:18193490-18193509 MS.gene28251:CDS 25.0%
! GCATTTCCAAAATAATCTTT+CGG + chr3.3:18193914-18193933 None:intergenic 25.0%
! GGAAAAAATGAATTGAGAAT+GGG + chr3.3:18193344-18193363 None:intergenic 25.0%
! TATGGTATTTCATTAATTCG+TGG - chr3.3:18193489-18193508 MS.gene28251:CDS 25.0%
! TGGAAAAAATGAATTGAGAA+TGG + chr3.3:18193345-18193364 None:intergenic 25.0%
! TGTAAATAATATAAGTGGAC+TGG - chr3.3:18194204-18194223 MS.gene28251:CDS 25.0%
!! CTAAAATATGTGTTTTCTTG+CGG + chr3.3:18193851-18193870 None:intergenic 25.0%
!! GATGTTTATGGCAATAATTT+TGG - chr3.3:18194154-18194173 MS.gene28251:CDS 25.0%
!! GCAAGAAAACACATATTTTA+GGG - chr3.3:18193850-18193869 MS.gene28251:CDS 25.0%
!! TAAAATATGTGTTTTCTTGC+GGG + chr3.3:18193850-18193869 None:intergenic 25.0%
!! TTATGGCAATAATTTTGGAT+GGG - chr3.3:18194159-18194178 MS.gene28251:CDS 25.0%
!! TTTATGGCAATAATTTTGGA+TGG - chr3.3:18194158-18194177 MS.gene28251:CDS 25.0%
!! TTTTGTAGTTGCTTAATGAA+GGG + chr3.3:18193611-18193630 None:intergenic 25.0%
!!! AATTCATTTTTTCCACTGAA+TGG - chr3.3:18193350-18193369 MS.gene28251:CDS 25.0%
AAAAATGAATTGAGAATGGG+TGG + chr3.3:18193341-18193360 None:intergenic 30.0%
AAATGAAATAACCTCTCTGA+TGG + chr3.3:18193701-18193720 None:intergenic 30.0%
AATGAAATAACCTCTCTGAT+GGG + chr3.3:18193700-18193719 None:intergenic 30.0%
AATGGAATCAAAAACTACGA+AGG - chr3.3:18193368-18193387 MS.gene28251:CDS 30.0%
CGCAGTGTAAATAATATAAG+TGG - chr3.3:18194199-18194218 MS.gene28251:CDS 30.0%
CTACAAAAGAACAAATCTGA+CGG - chr3.3:18193623-18193642 MS.gene28251:CDS 30.0%
GAAGAATGACTATGAGAATT+GGG - chr3.3:18194048-18194067 MS.gene28251:CDS 30.0%
TACAAAAGAACAAATCTGAC+GGG - chr3.3:18193624-18193643 MS.gene28251:CDS 30.0%
TGAAGAATGACTATGAGAAT+TGG - chr3.3:18194047-18194066 MS.gene28251:CDS 30.0%
TGTTACTATTACCAAGTCTA+AGG + chr3.3:18194090-18194109 None:intergenic 30.0%
TTAATGAAGGGAAATCGTAT+GGG + chr3.3:18193599-18193618 None:intergenic 30.0%
TTGAAATTGAAAGCCAATGT+AGG - chr3.3:18193743-18193762 MS.gene28251:CDS 30.0%
TTGAGTGCAAAGAAATCATT+TGG + chr3.3:18194018-18194037 None:intergenic 30.0%
! CGCAAGAAAACACATATTTT+AGG - chr3.3:18193849-18193868 MS.gene28251:CDS 30.0%
! CTTTTGTAGTTGCTTAATGA+AGG + chr3.3:18193612-18193631 None:intergenic 30.0%
! GAGGTTATTTCATTTCACAA+AGG - chr3.3:18193706-18193725 MS.gene28251:CDS 30.0%
! TATGGCAATAATTTTGGATG+GGG - chr3.3:18194160-18194179 MS.gene28251:CDS 30.0%
! TTGAAGGGAAGATATTTTGT+TGG + chr3.3:18193777-18193796 None:intergenic 30.0%
!! TAGTTTTTGATTCCATTCAG+TGG + chr3.3:18193365-18193384 None:intergenic 30.0%
!! TATGGTCTATTATTTCTAGC+CGG + chr3.3:18193208-18193227 None:intergenic 30.0%
!! TATTTTGGAAATGCTGTTGT+AGG - chr3.3:18193920-18193939 MS.gene28251:CDS 30.0%
!! TGGTTTTACTTTTAACCATC+TGG - chr3.3:18193448-18193467 MS.gene28251:CDS 30.0%
AAATGCTGTTGTAGGTTCTA+TGG - chr3.3:18193928-18193947 MS.gene28251:CDS 35.0%
AACTCTCAACACTTAAACGT+TGG - chr3.3:18193552-18193571 MS.gene28251:CDS 35.0%
AATAGCCAACACACGATAAT+CGG + chr3.3:18194116-18194135 None:intergenic 35.0%
AATCCAATCATCCTTAGACT+TGG - chr3.3:18194076-18194095 MS.gene28251:CDS 35.0%
ATTGAAAGCCAATGTAGGAA+TGG - chr3.3:18193748-18193767 MS.gene28251:CDS 35.0%
ATTGAGAATGGGTGGAATAT+AGG + chr3.3:18193333-18193352 None:intergenic 35.0%
CCAAAATAATCTTTCGGCAA+CGG + chr3.3:18193908-18193927 None:intergenic 35.0%
CCTCTCTACAGATACAATAA+AGG - chr3.3:18193010-18193029 MS.gene28251:CDS 35.0%
CCTTTATTGTATCTGTAGAG+AGG + chr3.3:18193013-18193032 None:intergenic 35.0%
CTATGGTTGCAATGAAAGTT+GGG - chr3.3:18193945-18193964 MS.gene28251:CDS 35.0%
CTTAATGAAGGGAAATCGTA+TGG + chr3.3:18193600-18193619 None:intergenic 35.0%
TATGGTTGCAATGAAAGTTG+GGG - chr3.3:18193946-18193965 MS.gene28251:CDS 35.0%
TCTATGGTTGCAATGAAAGT+TGG - chr3.3:18193944-18193963 MS.gene28251:CDS 35.0%
TGGAATAGAAGAAGGTAGTA+TGG - chr3.3:18194240-18194259 MS.gene28251:CDS 35.0%
TTACCAAGTCTAAGGATGAT+TGG + chr3.3:18194082-18194101 None:intergenic 35.0%
TTAGTTGACGGCATCTTTAT+TGG - chr3.3:18193428-18193447 MS.gene28251:CDS 35.0%
TTGAAAGCCAATGTAGGAAT+GGG - chr3.3:18193749-18193768 MS.gene28251:CDS 35.0%
TTTAACCATCTGGTTGTTGA+TGG - chr3.3:18193458-18193477 MS.gene28251:CDS 35.0%
! GGGAAATGATCATGTTTTCA+TGG - chr3.3:18194303-18194322 MS.gene28251:CDS 35.0%
! GTATTTGCTGGAATAGAAGA+AGG - chr3.3:18194232-18194251 MS.gene28251:CDS 35.0%
!! ACGTTTAAGTGTTGAGAGTT+TGG + chr3.3:18193552-18193571 None:intergenic 35.0%
!!! ACATATTTTAGGGTCTCGAT+AGG - chr3.3:18193860-18193879 MS.gene28251:CDS 35.0%
!!! AGTGTTTTAGCTGTCGAATT+TGG + chr3.3:18193145-18193164 None:intergenic 35.0%
AAAAACTCAACCCATCAGAG+AGG - chr3.3:18193687-18193706 MS.gene28251:CDS 40.0%
AAATAATCTTTCGGCAACGG+TGG + chr3.3:18193905-18193924 None:intergenic 40.0%
AAGCACTTTATGCACACCTT+TGG - chr3.3:18193795-18193814 MS.gene28251:CDS 40.0%
AAGTGTTCTCAATTGCAGCA+TGG + chr3.3:18193286-18193305 None:intergenic 40.0%
AAGTTCCATCAACAACCAGA+TGG + chr3.3:18193466-18193485 None:intergenic 40.0%
AATGAAAGTTGGGGAGTTGT+TGG - chr3.3:18193955-18193974 MS.gene28251:CDS 40.0%
ACTTGTGTAGGTGACATACT+TGG - chr3.3:18193302-18193321 MS.gene28251:CDS 40.0%
ATTGCCTCCAAAATCTTGTG+AGG + chr3.3:18194283-18194302 None:intergenic 40.0%
CAAGTCACAGAGTTAGTTGA+CGG - chr3.3:18193416-18193435 MS.gene28251:CDS 40.0%
GCAATTGAGAACACTTGTGT+AGG - chr3.3:18193290-18193309 MS.gene28251:CDS 40.0%
TCTCGATAGGAACTAGACAA+AGG - chr3.3:18193873-18193892 MS.gene28251:CDS 40.0%
TTGCCATAAACATCAAACCG+CGG + chr3.3:18194148-18194167 None:intergenic 40.0%
TTGTATCTGTAGAGAGGACT+CGG + chr3.3:18193007-18193026 None:intergenic 40.0%
! ACTCTAGAGGATGGTGATAA+AGG + chr3.3:18193118-18193137 None:intergenic 40.0%
! CCGTTGCCGAAAGATTATTT+TGG - chr3.3:18193905-18193924 MS.gene28251:CDS 40.0%
! GTGATAAAGGAGACCCTTTT+GGG + chr3.3:18193105-18193124 None:intergenic 40.0%
! GTGTCTTCCTCACAAGATTT+TGG - chr3.3:18194273-18194292 MS.gene28251:CDS 40.0%
! GTGTGCATAAAGTGCTTGAA+GGG + chr3.3:18193792-18193811 None:intergenic 40.0%
! TCTGGTTGTTGATGGAACTT+CGG - chr3.3:18193466-18193485 MS.gene28251:CDS 40.0%
! TCTTCCTCACAAGATTTTGG+AGG - chr3.3:18194276-18194295 MS.gene28251:CDS 40.0%
! TGTTTTCTTGCGGGTCTAAT+TGG + chr3.3:18193841-18193860 None:intergenic 40.0%
! TTGTTGATGGAACTTCGGTA+TGG - chr3.3:18193471-18193490 MS.gene28251:CDS 40.0%
!! ACGACATGGTGTTGTCTTTA+TGG + chr3.3:18193226-18193245 None:intergenic 40.0%
!! CACAAGATTTTGGAGGCAAT+GGG - chr3.3:18194283-18194302 MS.gene28251:CDS 40.0%
!! CGAATTTGGTTTGACTCTAG+AGG + chr3.3:18193131-18193150 None:intergenic 40.0%
!! CTTAAACGTTGGTTTCCAGA+TGG - chr3.3:18193563-18193582 MS.gene28251:CDS 40.0%
!! TCACAAGATTTTGGAGGCAA+TGG - chr3.3:18194282-18194301 MS.gene28251:CDS 40.0%
!! TTTGGTTTGACTCTAGAGGA+TGG + chr3.3:18193127-18193146 None:intergenic 40.0%
ACCAGCATTGTTGCATGTGA+CGG + chr3.3:18193252-18193271 None:intergenic 45.0%
AGTAGAACCTTGAGATCCCA+TGG + chr3.3:18193071-18193090 None:intergenic 45.0%
ATGGGACGTTGAATACCATC+TGG + chr3.3:18193581-18193600 None:intergenic 45.0%
CTTGTTGAACCCACCCAAAA+GGG - chr3.3:18193089-18193108 MS.gene28251:CDS 45.0%
GTTGGTACCCATTCCTACAT+TGG + chr3.3:18193759-18193778 None:intergenic 45.0%
TACGTCACAACCATTGCTTG+CGG - chr3.3:18193391-18193410 MS.gene28251:CDS 45.0%
TCTTACTCCATGGGATCTCA+AGG - chr3.3:18193061-18193080 MS.gene28251:CDS 45.0%
TGCCATAAACATCAAACCGC+GGG + chr3.3:18194147-18194166 None:intergenic 45.0%
TGTGAGGAAGACACACTTGA+AGG + chr3.3:18194267-18194286 None:intergenic 45.0%
TTCTTGCGGGTCTAATTGGT+CGG + chr3.3:18193837-18193856 None:intergenic 45.0%
! ACTTGTTGAACCCACCCAAA+AGG - chr3.3:18193088-18193107 MS.gene28251:CDS 45.0%
! ATAAAGGAGACCCTTTTGGG+TGG + chr3.3:18193102-18193121 None:intergenic 45.0%
! CATTGCCGATTATCGTGTGT+TGG - chr3.3:18194108-18194127 MS.gene28251:CDS 45.0%
! GGTGATAAAGGAGACCCTTT+TGG + chr3.3:18193106-18193125 None:intergenic 45.0%
! GGTGTGCATAAAGTGCTTGA+AGG + chr3.3:18193793-18193812 None:intergenic 45.0%
! TTTTGGATGGGGAAAACCTG+TGG - chr3.3:18194171-18194190 MS.gene28251:CDS 45.0%
!! GGACTGGTTGTTGTATTTGC+TGG - chr3.3:18194220-18194239 MS.gene28251:CDS 45.0%
!! TAAAGGAGACCCTTTTGGGT+GGG + chr3.3:18193101-18193120 None:intergenic 45.0%
ATCTGTAGAGAGGACTCGGA+TGG + chr3.3:18193003-18193022 None:intergenic 50.0%
GCCATAAACATCAAACCGCG+GGG + chr3.3:18194146-18194165 None:intergenic 50.0%
GTTGGGGAGTTGTTGGAAGA+AGG - chr3.3:18193962-18193981 MS.gene28251:CDS 50.0%
TAATTGGTCGGAGCGGATCA+CGG + chr3.3:18193825-18193844 None:intergenic 50.0%
TCCCCGCGGTTTGATGTTTA+TGG - chr3.3:18194142-18194161 MS.gene28251:CDS 50.0%
TCCGTCACATGCAACAATGC+TGG - chr3.3:18193248-18193267 MS.gene28251:CDS 50.0%
! TTGTTGGAAGAAGGCGCACT+TGG - chr3.3:18193971-18193990 MS.gene28251:CDS 50.0%
TGGATGGGGAAAACCTGTGG+CGG - chr3.3:18194174-18194193 MS.gene28251:CDS 55.0%
TGGCTATTAGCAGTTCCCCG+CGG - chr3.3:18194128-18194147 MS.gene28251:CDS 55.0%
TGGGCTGAAATCTCACGTGG+TGG - chr3.3:18193509-18193528 MS.gene28251:CDS 55.0%
TTTACACTGCGAACCGCCAC+AGG + chr3.3:18194190-18194209 None:intergenic 55.0%
! GCATGTGACGGAACACGACA+TGG + chr3.3:18193240-18193259 None:intergenic 55.0%
! GCCTGCAAGTGGTGGGAAAA+AGG + chr3.3:18193186-18193205 None:intergenic 55.0%
! GCCTTTTTCCCACCACTTGC+AGG - chr3.3:18193182-18193201 MS.gene28251:CDS 55.0%
! GTGACTTGCACCGCAAGCAA+TGG + chr3.3:18193404-18193423 None:intergenic 55.0%
! TCGTGGGCTGAAATCTCACG+TGG - chr3.3:18193506-18193525 MS.gene28251:CDS 55.0%
! TTTTCCCACCACTTGCAGGC+CGG - chr3.3:18193186-18193205 MS.gene28251:CDS 55.0%
!! ATTTCTAGCCGGCCTGCAAG+TGG + chr3.3:18193197-18193216 None:intergenic 55.0%
GCGGGTCTAATTGGTCGGAG+CGG + chr3.3:18193832-18193851 None:intergenic 60.0%
! AGCGGATCACGGAGCACCAA+AGG + chr3.3:18193814-18193833 None:intergenic 60.0%
CTAGCCGGCCTGCAAGTGGT+GGG + chr3.3:18193193-18193212 None:intergenic 65.0%
TCTAGCCGGCCTGCAAGTGG+TGG + chr3.3:18193194-18193213 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 18192993 18194342 18192993 ID=MS.gene28251
chr3.3 mRNA 18192993 18194342 18192993 ID=MS.gene28251.t1;Parent=MS.gene28251
chr3.3 exon 18192993 18194342 18192993 ID=MS.gene28251.t1.exon1;Parent=MS.gene28251.t1
chr3.3 CDS 18192993 18194342 18192993 ID=cds.MS.gene28251.t1;Parent=MS.gene28251.t1
Gene Sequence

>MS.gene28251

ATGGCTTCCATCCGAGTCCTCTCTACAGATACAATAAAGGCACCAAATTCAACTGATGAAAAAATTGATCTTACTCCATGGGATCTCAAGGTTCTACTTGTTGAACCCACCCAAAAGGGTCTCCTTTATCACCATCCTCTAGAGTCAAACCAAATTCGACAGCTAAAACACTCTCTATCTTCTGCTCTTGCCTTTTTCCCACCACTTGCAGGCCGGCTAGAAATAATAGACCATAAAGACAACACCATGTCGTGTTCCGTCACATGCAACAATGCTGGTGTACTCTTTGTCCATGCTGCAATTGAGAACACTTGTGTAGGTGACATACTTGGATGCACCTATATTCCACCCATTCTCAATTCATTTTTTCCACTGAATGGAATCAAAAACTACGAAGGTACGTCACAACCATTGCTTGCGGTGCAAGTCACAGAGTTAGTTGACGGCATCTTTATTGGTTTTACTTTTAACCATCTGGTTGTTGATGGAACTTCGGTATGGTATTTCATTAATTCGTGGGCTGAAATCTCACGTGGTGGTTGTTGCAATCAAATATCCAAACTCTCAACACTTAAACGTTGGTTTCCAGATGGTATTCAACGTCCCATACGATTTCCCTTCATTAAGCAACTACAAAAGAACAAATCTGACGGGTTGAGTTTTTCATCATCTAATAACGAAAAACTCAATAACGAAAAACTCAACCCATCAGAGAGGTTATTTCATTTCACAAAGGAGAAAATCTCTCAATTGAAATTGAAAGCCAATGTAGGAATGGGTACCAACAAAATATCTTCCCTTCAAGCACTTTATGCACACCTTTGGTGCTCCGTGATCCGCTCCGACCAATTAGACCCGCAAGAAAACACATATTTTAGGGTCTCGATAGGAACTAGACAAAGGTTTGTTCCACCGTTGCCGAAAGATTATTTTGGAAATGCTGTTGTAGGTTCTATGGTTGCAATGAAAGTTGGGGAGTTGTTGGAAGAAGGCGCACTTGGTAAATGTGCTTCGAAGATGCACCAAATGATTTCTTTGCACTCAAATGAGAAGTTGAAGAATGACTATGAGAATTGGGTGAGAAATCCAATCATCCTTAGACTTGGTAATAGTAACATTGCCGATTATCGTGTGTTGGCTATTAGCAGTTCCCCGCGGTTTGATGTTTATGGCAATAATTTTGGATGGGGAAAACCTGTGGCGGTTCGCAGTGTAAATAATATAAGTGGACTGGTTGTTGTATTTGCTGGAATAGAAGAAGGTAGTATGGACCTTCAAGTGTGTCTTCCTCACAAGATTTTGGAGGCAATGGGAAATGATCATGTTTTCATGGACTTTGTATCGAATTAA
Protein sequence

>MS.gene28251.t1

MASIRVLSTDTIKAPNSTDEKIDLTPWDLKVLLVEPTQKGLLYHHPLESNQIRQLKHSLSSALAFFPPLAGRLEIIDHKDNTMSCSVTCNNAGVLFVHAAIENTCVGDILGCTYIPPILNSFFPLNGIKNYEGTSQPLLAVQVTELVDGIFIGFTFNHLVVDGTSVWYFINSWAEISRGGCCNQISKLSTLKRWFPDGIQRPIRFPFIKQLQKNKSDGLSFSSSNNEKLNNEKLNPSERLFHFTKEKISQLKLKANVGMGTNKISSLQALYAHLWCSVIRSDQLDPQENTYFRVSIGTRQRFVPPLPKDYFGNAVVGSMVAMKVGELLEEGALGKCASKMHQMISLHSNEKLKNDYENWVRNPIILRLGNSNIADYRVLAISSSPRFDVYGNNFGWGKPVAVRSVNNISGLVVVFAGIEEGSMDLQVCLPHKILEAMGNDHVFMDFVSN