AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56796

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56796.t1 MTR_4g094835 71.681 452 120 3 1 450 1 446 0.0 673
MS.gene56796.t1 MTR_4g094838 71.429 455 101 7 4 451 1 433 0.0 632
MS.gene56796.t1 MTR_4g094832 72.149 377 94 3 81 451 60 431 0.0 555
MS.gene56796.t1 MTR_4g094842 59.912 454 164 8 1 446 1 444 0.0 537
MS.gene56796.t1 MTR_0190s0020 58.575 449 172 8 1 446 1 438 0.0 523
MS.gene56796.t1 MTR_8g041770 57.238 449 177 8 1 446 1 437 0.0 512
MS.gene56796.t1 MTR_3g088420 58.036 448 167 10 1 446 1 429 4.81e-177 502
MS.gene56796.t1 MTR_4g022720 56.793 449 173 8 1 446 1 431 2.25e-176 501
MS.gene56796.t1 MTR_4g094828 56.416 452 177 10 1 442 1 442 3.64e-176 501
MS.gene56796.t1 MTR_4g022825 55.947 454 174 10 1 446 1 436 4.88e-174 495
MS.gene56796.t1 MTR_8g079710 57.333 450 169 10 1 441 1 436 1.20e-173 494
MS.gene56796.t1 MTR_4g022800 55.482 456 175 10 1 446 1 438 4.41e-172 490
MS.gene56796.t1 MTR_4g022290 57.301 452 168 12 1 442 1 437 6.97e-172 489
MS.gene56796.t1 MTR_4g022670 54.505 455 180 10 1 446 1 437 1.92e-168 481
MS.gene56796.t1 MTR_3g026310 54.230 461 179 11 1 446 1 444 1.03e-167 479
MS.gene56796.t1 MTR_7g026860 54.185 454 180 10 1 446 1 434 2.05e-163 468
MS.gene56796.t1 MTR_4g022760 49.119 454 162 9 1 446 1 393 4.84e-140 407
MS.gene56796.t1 MTR_6g016815 42.634 448 170 13 1 446 15 377 9.38e-104 314
MS.gene56796.t1 MTR_4g094825 86.628 172 22 1 20 191 80 250 1.15e-96 296
MS.gene56796.t1 MTR_4g094825 84.516 155 14 1 297 451 248 392 8.24e-85 265
MS.gene56796.t1 MTR_3g101900 39.860 429 238 9 28 446 26 444 1.28e-93 290
MS.gene56796.t1 MTR_3g102020 38.178 461 250 11 3 446 5 447 2.52e-91 285
MS.gene56796.t1 MTR_3g101930 38.889 432 237 9 32 446 30 451 8.80e-90 280
MS.gene56796.t1 MTR_3g102010 38.126 459 250 11 4 446 7 447 7.77e-88 275
MS.gene56796.t1 MTR_3g101960 37.336 458 255 10 3 445 5 445 1.00e-86 273
MS.gene56796.t1 MTR_3g101970 37.412 425 236 8 32 446 29 433 3.83e-84 265
MS.gene56796.t1 MTR_5g081960 33.747 483 239 10 30 451 25 487 3.20e-80 256
MS.gene56796.t1 MTR_3g070170 35.652 460 237 10 30 450 31 470 6.79e-79 253
MS.gene56796.t1 MTR_8g040760 55.357 224 88 5 1 221 1 215 4.67e-76 237
MS.gene56796.t1 MTR_8g040780 57.971 207 82 3 239 442 20 224 7.56e-76 238
MS.gene56796.t1 MTR_4g015180 32.851 484 244 11 27 446 17 483 4.99e-70 230
MS.gene56796.t1 MTR_7g026850 55.233 172 70 3 37 206 12 178 2.70e-58 190
MS.gene56796.t1 MTR_6g045553 28.571 483 288 12 2 447 10 472 3.22e-49 174
MS.gene56796.t1 MTR_3g102030 33.007 306 168 4 103 404 48 320 2.53e-48 168
MS.gene56796.t1 MTR_7g026770 48.619 181 66 4 154 332 132 287 3.32e-45 159
MS.gene56796.t1 MTR_6g034485 55.385 130 53 2 199 328 1 125 3.65e-44 151
MS.gene56796.t1 MTR_6g034495 60.000 125 44 2 72 191 37 160 9.41e-44 151
MS.gene56796.t1 MTR_7g026830 68.293 82 26 0 97 178 16 97 3.48e-33 121
MS.gene56796.t1 MTR_4g007540 26.343 391 253 12 64 439 56 426 1.10e-28 117
MS.gene56796.t1 MTR_8g096530 29.444 360 214 13 57 402 52 385 4.38e-27 113
MS.gene56796.t1 MTR_3g098980 26.873 387 246 15 57 432 59 419 1.30e-26 111
MS.gene56796.t1 MTR_3g049150 25.459 436 271 13 37 437 21 437 1.71e-26 111
MS.gene56796.t1 MTR_2g105330 24.051 395 259 10 64 435 56 432 1.81e-25 108
MS.gene56796.t1 MTR_3g026950 23.529 408 258 12 61 435 51 437 4.81e-25 107
MS.gene56796.t1 MTR_2g064495 23.684 380 260 9 64 431 56 417 2.45e-24 105
MS.gene56796.t1 MTR_8g075610 24.619 394 252 15 64 439 47 413 1.10e-23 103
MS.gene56796.t1 MTR_4g097090 24.943 437 259 16 40 431 28 440 2.86e-23 102
MS.gene56796.t1 MTR_3g071320 27.027 333 202 12 49 359 42 355 2.29e-22 99.4
MS.gene56796.t1 MTR_4g127650 22.624 442 288 14 22 431 21 440 4.50e-22 98.6
MS.gene56796.t1 MTR_1g081660 24.036 441 293 16 32 450 31 451 2.77e-21 96.3
MS.gene56796.t1 MTR_7g027175 26.154 390 258 12 57 437 61 429 2.87e-21 96.3
MS.gene56796.t1 MTR_7g026780 46.296 108 32 3 339 446 26 107 7.92e-21 87.8
MS.gene56796.t1 MTR_6g015850 24.197 467 302 15 2 439 6 449 1.02e-19 91.7
MS.gene56796.t1 MTR_6g015815 23.059 438 292 13 30 439 32 452 2.46e-19 90.5
MS.gene56796.t1 MTR_5g084870 26.076 395 230 17 33 400 17 376 4.00e-19 89.7
MS.gene56796.t1 MTR_7g026800 37.654 162 69 6 228 387 29 160 8.22e-19 84.7
MS.gene56796.t1 MTR_2g089765 25.417 480 283 21 3 434 15 467 8.35e-19 89.0
MS.gene56796.t1 MTR_6g015315 26.250 320 199 8 152 439 141 455 9.13e-19 88.6
MS.gene56796.t1 MTR_3g087640 26.419 458 277 20 30 444 29 469 9.66e-19 89.0
MS.gene56796.t1 MTR_5g026320 25.182 413 246 16 61 445 71 448 1.15e-18 88.6
MS.gene56796.t1 MTR_7g084940 25.967 362 228 12 52 399 57 392 3.26e-18 87.0
MS.gene56796.t1 MTR_1g040195 23.864 440 257 18 33 444 23 412 3.34e-18 87.0
MS.gene56796.t1 MTR_4g022780 36.364 165 73 6 228 390 30 164 3.41e-18 82.4
MS.gene56796.t1 MTR_2g089755 26.585 410 244 20 32 400 37 430 3.82e-18 87.0
MS.gene56796.t1 MTR_3g086020 25.558 403 242 17 62 451 59 416 9.23e-18 85.5
MS.gene56796.t1 MTR_8g012750 26.316 418 241 18 30 399 24 422 9.43e-18 85.9
MS.gene56796.t1 MTR_7g014330 25.000 408 246 16 30 399 24 409 1.20e-17 85.5
MS.gene56796.t1 MTR_8g079980 24.433 397 239 11 33 400 17 381 2.31e-17 84.3
MS.gene56796.t1 MTR_6g015320 26.667 315 188 12 152 437 144 444 2.40e-17 84.3
MS.gene56796.t1 MTR_7g014250 25.783 415 234 18 30 399 24 409 2.94e-17 84.3
MS.gene56796.t1 MTR_3g049140 26.872 227 144 6 227 437 14 234 3.15e-17 81.6
MS.gene56796.t1 MTR_1g040175 23.601 411 272 18 48 439 45 432 9.36e-17 82.8
MS.gene56796.t1 MTR_2g089670 24.515 412 246 17 29 399 42 429 9.91e-17 82.8
MS.gene56796.t1 MTR_7g085930 23.961 409 249 14 64 426 59 451 1.91e-16 81.6
MS.gene56796.t1 MTR_5g076700 22.398 442 287 16 33 444 24 439 2.39e-16 81.3
MS.gene56796.t1 MTR_7g114470 24.138 435 269 18 33 434 25 431 4.57e-16 80.5
MS.gene56796.t1 MTR_7g086000 25.854 410 254 14 62 439 58 449 6.06e-16 80.1
MS.gene56796.t1 MTR_4g055170 24.252 301 208 6 64 358 69 355 1.86e-15 78.6
MS.gene56796.t1 MTR_4g013050 21.814 408 247 12 57 441 46 404 2.05e-15 78.2
MS.gene56796.t1 MTR_2g089650 24.450 409 268 18 23 400 20 418 1.07e-14 76.3
MS.gene56796.t1 MTR_3g450710 25.648 386 218 16 58 404 59 414 1.33e-14 76.3
MS.gene56796.t1 MTR_5g090810 25.337 371 225 14 61 400 63 412 3.08e-14 75.1
MS.gene56796.t1 MTR_7g114520 24.041 391 248 19 37 407 41 402 3.49e-14 74.7
MS.gene56796.t1 MTR_7g114490 24.074 432 257 20 37 437 40 431 3.66e-14 74.7
MS.gene56796.t1 MTR_7g014160 23.044 473 285 19 17 431 4 455 5.79e-14 73.9
MS.gene56796.t1 MTR_7g014230 23.704 405 255 15 30 399 26 411 6.23e-14 73.9
MS.gene56796.t1 MTR_7g014610 23.974 463 273 20 23 431 20 457 7.48e-14 73.9
MS.gene56796.t1 MTR_7g014200 24.885 434 235 18 23 399 21 420 9.28e-14 73.6
MS.gene56796.t1 MTR_7g056087 23.153 406 267 15 40 416 30 419 2.02e-13 72.4
MS.gene56796.t1 MTR_1g040315 24.333 300 194 10 33 317 23 304 2.42e-13 72.0
MS.gene56796.t1 MTR_1g053315 21.652 351 243 13 64 400 78 410 2.47e-13 72.0
MS.gene56796.t1 MTR_7g085990 23.188 414 246 15 64 426 55 447 2.54e-13 72.0
MS.gene56796.t1 MTR_5g090880 24.339 378 229 16 57 400 60 414 5.29e-13 71.2
MS.gene56796.t1 MTR_8g467580 26.682 431 244 21 49 446 41 432 8.41e-13 70.5
MS.gene56796.t1 MTR_3g028210 24.516 465 290 23 4 431 8 448 1.05e-12 70.1
MS.gene56796.t1 MTR_7g084910 24.503 302 180 9 42 316 38 318 1.12e-12 70.1
MS.gene56796.t1 MTR_6g053810 23.569 297 188 10 64 342 71 346 2.21e-12 69.3
MS.gene56796.t1 MTR_7g013850 22.410 415 251 14 37 399 37 432 5.31e-12 68.2
MS.gene56796.t1 MTR_7g026880 57.778 45 19 0 402 446 5 49 1.00e-11 60.1
MS.gene56796.t1 MTR_1g094790 24.499 449 254 22 40 446 32 437 2.76e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56796.t1 AT5G67150 41.280 453 245 7 2 446 3 442 2.75e-116 348
MS.gene56796.t1 AT3G50300 40.351 456 249 9 1 446 1 443 6.62e-103 314
MS.gene56796.t1 AT3G50280 37.251 451 266 7 1 446 1 439 2.92e-98 302
MS.gene56796.t1 AT3G50270 39.462 446 257 8 6 446 7 444 1.49e-97 300
MS.gene56796.t1 AT5G38130 39.492 433 246 7 5 431 20 442 6.37e-97 299
MS.gene56796.t1 AT5G67160 39.202 426 234 8 25 445 22 427 6.89e-96 295
MS.gene56796.t1 AT5G42830 35.963 431 253 9 28 446 26 445 9.72e-84 265
MS.gene56796.t1 AT2G39980 33.121 471 240 9 30 446 27 476 2.03e-79 254
MS.gene56796.t1 AT5G07860 37.037 432 238 11 32 446 31 445 3.56e-79 253
MS.gene56796.t1 AT5G07850 36.512 430 247 10 32 446 31 449 2.66e-78 251
MS.gene56796.t1 AT5G07870 35.455 440 246 9 32 446 31 457 3.00e-73 238
MS.gene56796.t1 AT5G01210 33.333 450 246 8 36 446 31 465 3.02e-73 238
MS.gene56796.t1 AT3G50280 38.462 312 186 3 138 446 1 309 8.48e-71 227
MS.gene56796.t1 AT5G23940 32.258 434 275 8 28 447 21 449 6.01e-62 209
MS.gene56796.t1 AT3G50290 39.552 268 155 3 135 397 4 269 6.78e-56 187
MS.gene56796.t1 AT2G19070 25.799 438 279 12 35 439 19 443 1.25e-27 115
MS.gene56796.t1 AT3G29590 25.682 440 288 13 24 441 20 442 1.20e-26 112
MS.gene56796.t1 AT3G48720 26.420 352 231 12 57 400 54 385 1.78e-25 108
MS.gene56796.t1 AT5G48930 24.631 406 263 11 49 434 39 421 1.58e-24 105
MS.gene56796.t1 AT5G41040 26.031 388 251 11 57 431 62 426 2.12e-24 105
MS.gene56796.t1 AT5G41040 26.031 388 251 11 57 431 78 442 3.20e-24 105
MS.gene56796.t1 AT5G57840 28.641 412 237 18 49 431 39 422 1.96e-22 99.8
MS.gene56796.t1 AT1G78990 27.642 369 224 13 51 400 52 396 6.38e-22 98.2
MS.gene56796.t1 AT3G29635 24.537 432 276 15 30 431 27 438 2.16e-21 96.7
MS.gene56796.t1 AT1G24430 23.622 381 243 14 55 423 53 397 7.13e-20 92.0
MS.gene56796.t1 AT5G63560 24.227 388 252 14 57 431 54 412 1.19e-19 91.3
MS.gene56796.t1 AT3G29670 24.503 453 297 18 3 431 2 433 5.36e-19 89.4
MS.gene56796.t1 AT1G31490 26.225 408 256 16 61 451 59 438 1.10e-18 88.6
MS.gene56796.t1 AT5G39050 24.920 313 200 12 148 432 145 450 3.97e-18 87.0
MS.gene56796.t1 AT5G23970 24.480 433 280 17 28 447 20 418 4.95e-18 86.3
MS.gene56796.t1 AT3G03480 24.450 409 257 18 33 419 33 411 1.90e-17 84.7
MS.gene56796.t1 AT5G17540 23.918 439 283 18 33 444 23 437 3.26e-17 84.0
MS.gene56796.t1 AT2G40230 24.396 414 272 13 1 400 1 387 3.37e-17 84.0
MS.gene56796.t1 AT1G24420 25.128 390 244 16 74 450 75 429 7.08e-17 82.8
MS.gene56796.t1 AT1G24420 25.128 390 244 16 74 450 75 429 7.08e-17 82.8
MS.gene56796.t1 AT3G26040 24.257 404 256 14 60 446 55 425 1.45e-16 82.0
MS.gene56796.t1 AT3G30280 24.706 425 256 20 1 400 1 386 1.67e-16 82.0
MS.gene56796.t1 AT5G47950 22.807 456 301 15 1 446 1 415 3.71e-16 80.9
MS.gene56796.t1 AT1G28680 25.882 255 171 6 154 400 143 387 2.07e-15 78.6
MS.gene56796.t1 AT5G61160 26.688 311 196 14 148 437 139 438 2.11e-15 78.6
MS.gene56796.t1 AT5G07080 25.649 308 190 12 42 330 44 331 4.55e-15 77.4
MS.gene56796.t1 AT1G28680 26.190 210 142 4 154 356 143 346 4.51e-14 73.9
MS.gene56796.t1 AT3G47170 24.342 304 194 11 57 338 63 352 5.83e-14 74.3
MS.gene56796.t1 AT4G15390 23.580 352 222 15 4 344 8 323 6.50e-14 73.9
MS.gene56796.t1 AT4G15390 23.580 352 222 15 4 344 8 323 6.50e-14 73.9
MS.gene56796.t1 AT5G16410 24.167 360 223 14 62 400 89 419 3.43e-13 71.6
MS.gene56796.t1 AT3G29680 23.356 441 281 17 23 431 16 431 4.17e-13 71.2
MS.gene56796.t1 AT2G25150 23.056 360 244 12 59 403 62 403 3.87e-12 68.6
MS.gene56796.t1 AT1G27620 22.539 386 267 12 64 439 58 421 1.04e-11 67.0
MS.gene56796.t1 AT1G65450 22.067 358 246 12 55 399 63 400 1.86e-11 66.2
MS.gene56796.t1 AT5G47980 25.061 411 243 20 63 446 60 432 6.00e-11 64.7

Find 95 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGATTCTTTCAGCTAAAT+TGG 0.161367 8.2:-25490216 None:intergenic
GTGTCTTTCTTATGAGATTT+TGG 0.228049 8.2:+25490799 MS.gene56796:CDS
CGTTGAAATGAAGGGAGTTT+TGG 0.257934 8.2:-25490101 None:intergenic
GGAAACGCGGTAGTGGTTAA+TGG 0.258596 8.2:+25490455 MS.gene56796:CDS
TCATTCTTCCCACCCTTTGC+AGG 0.261438 8.2:+25489729 MS.gene56796:CDS
TACATTCTGCTGAGAAAATA+AGG 0.265034 8.2:+25490555 MS.gene56796:CDS
TATGGGAACCTTTCGAGATT+TGG 0.266155 8.2:-25490073 None:intergenic
GGGAAATGATGTTGAGTTCT+TGG 0.297726 8.2:+25490829 MS.gene56796:CDS
TTTGGCATTTCGTTAATTCT+TGG 0.304274 8.2:+25490048 MS.gene56796:CDS
ATGGGAACCTTTCGAGATTT+GGG 0.326542 8.2:-25490072 None:intergenic
GGTGGAAAAGAAGGCCTCTT+TGG 0.329077 8.2:-25489647 None:intergenic
TGAGATGGTTGATTTGGTTT+TGG 0.333677 8.2:-25489683 None:intergenic
TGATGTGTTGTTGTTGTTTG+TGG 0.353296 8.2:-25489781 None:intergenic
TAAGGAACCATTACGAATCT+TGG 0.357996 8.2:+25490573 MS.gene56796:CDS
TTTGTTTGAGATGGTTGATT+TGG 0.369144 8.2:-25489689 None:intergenic
ACCAAGGATGATTCCACCTT+TGG 0.373650 8.2:+25490418 MS.gene56796:CDS
ATTGGAATTTCAATATCGTT+TGG 0.377241 8.2:-25490131 None:intergenic
TTGGAATTTCAATATCGTTT+GGG 0.377876 8.2:-25490130 None:intergenic
AAGTACTATTATCTCATGTT+TGG 0.384120 8.2:+25490324 MS.gene56796:CDS
TTGTAGACGCCAAGTCGTTT+TGG 0.392431 8.2:+25490030 MS.gene56796:CDS
AGCTTTCTTCTGAGGAGTCT+TGG 0.397249 8.2:-25490187 None:intergenic
TGTTGTGGATATCACCTTGA+TGG 0.409513 8.2:-25489520 None:intergenic
AAAACCAACTCTAGCAATTC+AGG 0.414621 8.2:+25489956 MS.gene56796:CDS
TTAAGTGACGGCATCTTCAT+TGG 0.417429 8.2:+25489987 MS.gene56796:CDS
CCTTTGGAAGAAGATTATTT+CGG 0.422258 8.2:+25490434 MS.gene56796:CDS
TGTGTGAAAGGGAAGCGTAT+TGG 0.429614 8.2:-25490149 None:intergenic
CAGTCACATTACTTAGAACT+AGG 0.433385 8.2:-25490854 None:intergenic
GTTTGTGGTGTCGTTGTGTT+GGG 0.434146 8.2:-25489766 None:intergenic
CATTCTTCCCACCCTTTGCA+GGG 0.439523 8.2:+25489730 MS.gene56796:CDS
TCCAAAGGTGGAATCATCCT+TGG 0.454320 8.2:-25490419 None:intergenic
TGTTTGTGGTGTCGTTGTGT+TGG 0.462700 8.2:-25489767 None:intergenic
TTTGGTTAGGAATGGGTTGG+TGG 0.465905 8.2:-25489665 None:intergenic
CCGAAATAATCTTCTTCCAA+AGG 0.467292 8.2:-25490434 None:intergenic
AGATTATTATTGTTGTCAAT+TGG 0.472545 8.2:-25489555 None:intergenic
GGGAACCAACGTTGAAATGA+AGG 0.474068 8.2:-25490110 None:intergenic
GACAATTGGAGGTGTGTAGT+TGG 0.479530 8.2:-25489886 None:intergenic
TGGAAAGTGTTTGTTTGAGA+TGG 0.485281 8.2:-25489698 None:intergenic
TTCTCAACCAAGATTCGTAA+TGG 0.489014 8.2:-25490580 None:intergenic
GAGGTTCTAGAGTGTGAAAT+CGG 0.491146 8.2:+25490497 MS.gene56796:CDS
AACGAAATGCCAAAACGACT+TGG 0.492743 8.2:-25490039 None:intergenic
GAATGGGTTGGTGGAAAAGA+AGG 0.503869 8.2:-25489656 None:intergenic
TGTGATTGTATTTGTGTGAA+AGG 0.505410 8.2:-25490161 None:intergenic
TTATTTCGGAAACGCGGTAG+TGG 0.506417 8.2:+25490448 MS.gene56796:CDS
CTTCTTCATGGATTCCATCA+AGG 0.511613 8.2:+25489506 None:intergenic
AATGGTGTGAGGATGAAAGC+AGG 0.512088 8.2:+25490473 MS.gene56796:CDS
GTTACCTGAATTGCTAGAGT+TGG 0.515092 8.2:-25489960 None:intergenic
TGGTTTCAAGTGGAAGGAAT+CGG 0.519621 8.2:-25489623 None:intergenic
GAGGTGTGTAGTTGGATTGA+AGG 0.522441 8.2:-25489878 None:intergenic
TTTGGATGGTTTCAAGTGGA+AGG 0.524015 8.2:-25489629 None:intergenic
GAAAAGAAGGCCTCTTTGGA+TGG 0.529505 8.2:-25489643 None:intergenic
GTGATTGTATTTGTGTGAAA+GGG 0.534325 8.2:-25490160 None:intergenic
CTCCCACTTCGAAACGCCAC+AGG 0.534418 8.2:-25490707 None:intergenic
GGATTCTAAGCTTTCTTCTG+AGG 0.535259 8.2:-25490195 None:intergenic
TTCCGAAGTGGTAGTTCGCT+AGG 0.540203 8.2:+25490614 MS.gene56796:CDS
ACCGAGGTTGTTGCAAATGA+TGG 0.541381 8.2:-25489808 None:intergenic
GATTACAAGACGCCCTGCAA+AGG 0.543778 8.2:-25489742 None:intergenic
AGCCTGTGGCGTTTCGAAGT+GGG 0.545492 8.2:+25490705 MS.gene56796:CDS
AACTCCCTTCATTTCAACGT+TGG 0.545872 8.2:+25490105 MS.gene56796:CDS
GTGGCGTTTCGAAGTGGGAG+TGG 0.549176 8.2:+25490710 MS.gene56796:CDS
ATGGTGTGAGGATGAAAGCA+GGG 0.551554 8.2:+25490474 MS.gene56796:CDS
GTGTTTGCTGGTGCTGAAGA+AGG 0.553326 8.2:+25490758 MS.gene56796:CDS
ATTACAAGACGCCCTGCAAA+GGG 0.555357 8.2:-25489741 None:intergenic
TTCGCTAGGTGGTAATATGT+TGG 0.564328 8.2:+25490628 MS.gene56796:CDS
AAATAATCTTCTTCCAAAGG+TGG 0.566011 8.2:-25490431 None:intergenic
TGGTGCTTGAATTGTTGTTG+TGG 0.571268 8.2:-25489535 None:intergenic
GGAACCAACGTTGAAATGAA+GGG 0.576282 8.2:-25490109 None:intergenic
TCTTGGTCCCAAATCTCGAA+AGG 0.578370 8.2:+25490065 MS.gene56796:CDS
AACTCTAGCAATTCAGGTAA+CGG 0.581378 8.2:+25489962 MS.gene56796:CDS
GAAAATTGATCTAACACCAT+GGG 0.583475 8.2:+25489596 MS.gene56796:CDS
CACCTAGCGAACTACCACTT+CGG 0.583535 8.2:-25490616 None:intergenic
CCTCTTTGGATGGTTTCAAG+TGG 0.585328 8.2:-25489633 None:intergenic
TCCATCATTTGCAACAACCT+CGG 0.586818 8.2:+25489807 MS.gene56796:CDS
ACATAGAAAACATCTTCTTG+TGG 0.596961 8.2:-25490377 None:intergenic
GGGTGGGAAGAATGAGAGTG+TGG 0.597075 8.2:-25489721 None:intergenic
GGAAGGAATCGGAGATCCCA+TGG 0.597088 8.2:-25489612 None:intergenic
AAGCCTGTGGCGTTTCGAAG+TGG 0.599152 8.2:+25490704 MS.gene56796:CDS
ACAAGACGCCCTGCAAAGGG+TGG 0.600540 8.2:-25489738 None:intergenic
ACACCAACGTTGTTCCGAAG+TGG 0.610004 8.2:+25490602 MS.gene56796:CDS
TGGGAAGAATGAGAGTGTGG+TGG 0.610635 8.2:-25489718 None:intergenic
AGAAAATTGATCTAACACCA+TGG 0.626932 8.2:+25489595 MS.gene56796:CDS
CCACTTGAAACCATCCAAAG+AGG 0.628828 8.2:+25489633 MS.gene56796:CDS
CTACCACTTCGGAACAACGT+TGG 0.629293 8.2:-25490605 None:intergenic
CAAGACGCCCTGCAAAGGGT+GGG 0.638377 8.2:-25489737 None:intergenic
AAGAATCTTCCATTTCACAA+AGG 0.647468 8.2:+25490229 MS.gene56796:CDS
TGGTGTGAGGATGAAAGCAG+GGG 0.647651 8.2:+25490475 MS.gene56796:CDS
CGAAGTGGTAGTTCGCTAGG+TGG 0.653912 8.2:+25490617 MS.gene56796:CDS
CAGGTAACGGAGTTAAGTGA+CGG 0.655221 8.2:+25489975 MS.gene56796:CDS
AGCACAAAACACAACTGTCG+CGG 0.659280 8.2:+25489851 MS.gene56796:CDS
TGTGAGGATGAAAGCAGGGG+AGG 0.662636 8.2:+25490478 MS.gene56796:CDS
CGGTAGTGGTTAATGGTGTG+AGG 0.683331 8.2:+25490462 MS.gene56796:CDS
TTTCACAAAGGAGAAAATCG+CGG 0.713213 8.2:+25490241 MS.gene56796:CDS
AGAAGATTATTTCGGAAACG+CGG 0.717549 8.2:+25490442 MS.gene56796:CDS
ACTTGGCGTCTACAACAGCG+TGG 0.778771 8.2:-25490022 None:intergenic
TGATGATAGATGTTAGACCA+AGG 0.780037 8.2:+25490402 MS.gene56796:CDS
CGTGGACGAAGAGTGCACCG+AGG 0.794757 8.2:-25489824 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTAGATTTATCTGTTT+CGG - chr8.2:25490294-25490313 None:intergenic 15.0%
!! AGATTATTATTGTTGTCAAT+TGG - chr8.2:25489558-25489577 None:intergenic 20.0%
! AAGTACTATTATCTCATGTT+TGG + chr8.2:25490324-25490343 MS.gene56796:CDS 25.0%
! ATTGGAATTTCAATATCGTT+TGG - chr8.2:25490134-25490153 None:intergenic 25.0%
! TTGGAATTTCAATATCGTTT+GGG - chr8.2:25490133-25490152 None:intergenic 25.0%
!!! CACTCATTTTTTACACTTAA+CGG + chr8.2:25489909-25489928 MS.gene56796:CDS 25.0%
AAATAATCTTCTTCCAAAGG+TGG - chr8.2:25490434-25490453 None:intergenic 30.0%
AAGAATCTTCCATTTCACAA+AGG + chr8.2:25490229-25490248 MS.gene56796:CDS 30.0%
ACATAGAAAACATCTTCTTG+TGG - chr8.2:25490380-25490399 None:intergenic 30.0%
AGAAAATTGATCTAACACCA+TGG + chr8.2:25489595-25489614 MS.gene56796:CDS 30.0%
CGTTAAGTGTAAAAAATGAG+TGG - chr8.2:25489911-25489930 None:intergenic 30.0%
GAAAATTGATCTAACACCAT+GGG + chr8.2:25489596-25489615 MS.gene56796:CDS 30.0%
GAAGATTCTTTCAGCTAAAT+TGG - chr8.2:25490219-25490238 None:intergenic 30.0%
GGGAGTGGTAATAAAAATAA+TGG + chr8.2:25490725-25490744 MS.gene56796:CDS 30.0%
GTAAAAAATGAGTGGACAAT+TGG - chr8.2:25489903-25489922 None:intergenic 30.0%
GTGATTGTATTTGTGTGAAA+GGG - chr8.2:25490163-25490182 None:intergenic 30.0%
TACATTCTGCTGAGAAAATA+AGG + chr8.2:25490555-25490574 MS.gene56796:CDS 30.0%
TGTGATTGTATTTGTGTGAA+AGG - chr8.2:25490164-25490183 None:intergenic 30.0%
TTGCCAAAAACATCAAATCT+CGG - chr8.2:25490668-25490687 None:intergenic 30.0%
TTTGGCATTTCGTTAATTCT+TGG + chr8.2:25490048-25490067 MS.gene56796:CDS 30.0%
! CCTTTGGAAGAAGATTATTT+CGG + chr8.2:25490434-25490453 MS.gene56796:CDS 30.0%
! GTGTCTTTCTTATGAGATTT+TGG + chr8.2:25490799-25490818 MS.gene56796:CDS 30.0%
! TCTTTCTTATGAGATTTTGG+AGG + chr8.2:25490802-25490821 MS.gene56796:CDS 30.0%
! TTTGTTTGAGATGGTTGATT+TGG - chr8.2:25489692-25489711 None:intergenic 30.0%
!! AAGGGAGTTTTGGTATTTTA+TGG - chr8.2:25490094-25490113 None:intergenic 30.0%
!! AGGGAGTTTTGGTATTTTAT+GGG - chr8.2:25490093-25490112 None:intergenic 30.0%
!! TTTTTGGCAATGATTTTGGT+TGG + chr8.2:25490678-25490697 MS.gene56796:CDS 30.0%
!!! ATTTGGTTTTGGTTAGGAAT+GGG - chr8.2:25489675-25489694 None:intergenic 30.0%
!!! GATGTTTTTGGCAATGATTT+TGG + chr8.2:25490674-25490693 MS.gene56796:CDS 30.0%
!!! TTTTGGCAATGATTTTGGTT+GGG + chr8.2:25490679-25490698 MS.gene56796:CDS 30.0%
AAAAATGAGTGGACAATTGG+AGG - chr8.2:25489900-25489919 None:intergenic 35.0%
AAAACCAACTCTAGCAATTC+AGG + chr8.2:25489956-25489975 MS.gene56796:CDS 35.0%
AACTCTAGCAATTCAGGTAA+CGG + chr8.2:25489962-25489981 MS.gene56796:CDS 35.0%
AGAAGATTATTTCGGAAACG+CGG + chr8.2:25490442-25490461 MS.gene56796:CDS 35.0%
CCGAAATAATCTTCTTCCAA+AGG - chr8.2:25490437-25490456 None:intergenic 35.0%
GGAAAAATTAGTGTGTTTGC+TGG + chr8.2:25490746-25490765 MS.gene56796:CDS 35.0%
TAAGGAACCATTACGAATCT+TGG + chr8.2:25490573-25490592 MS.gene56796:CDS 35.0%
TGATGATAGATGTTAGACCA+AGG + chr8.2:25490402-25490421 MS.gene56796:CDS 35.0%
TTCTCAACCAAGATTCGTAA+TGG - chr8.2:25490583-25490602 None:intergenic 35.0%
TTTCACAAAGGAGAAAATCG+CGG + chr8.2:25490241-25490260 MS.gene56796:CDS 35.0%
! CGATTTTCTCCTTTGTGAAA+TGG - chr8.2:25490241-25490260 None:intergenic 35.0%
! TCTCCGAGATTTGATGTTTT+TGG + chr8.2:25490662-25490681 MS.gene56796:CDS 35.0%
!! TATGAGATTTTGGAGGCTTT+GGG + chr8.2:25490809-25490828 MS.gene56796:CDS 35.0%
!! TGATGTGTTGTTGTTGTTTG+TGG - chr8.2:25489784-25489803 None:intergenic 35.0%
!! TGGAAAGTGTTTGTTTGAGA+TGG - chr8.2:25489701-25489720 None:intergenic 35.0%
!! TTATGAGATTTTGGAGGCTT+TGG + chr8.2:25490808-25490827 MS.gene56796:CDS 35.0%
!!! GATTTGGTTTTGGTTAGGAA+TGG - chr8.2:25489676-25489695 None:intergenic 35.0%
!!! TGAGATGGTTGATTTGGTTT+TGG - chr8.2:25489686-25489705 None:intergenic 35.0%
!!! TGGTTGATTTGGTTTTGGTT+AGG - chr8.2:25489681-25489700 None:intergenic 35.0%
!!! TTTGGCAATGATTTTGGTTG+GGG + chr8.2:25490680-25490699 MS.gene56796:CDS 35.0%
AACGAAATGCCAAAACGACT+TGG - chr8.2:25490042-25490061 None:intergenic 40.0%
AACTCCCTTCATTTCAACGT+TGG + chr8.2:25490105-25490124 MS.gene56796:CDS 40.0%
ATGGGAACCTTTCGAGATTT+GGG - chr8.2:25490075-25490094 None:intergenic 40.0%
GGAACCAACGTTGAAATGAA+GGG - chr8.2:25490112-25490131 None:intergenic 40.0%
GGATTCTAAGCTTTCTTCTG+AGG - chr8.2:25490198-25490217 None:intergenic 40.0%
GGGAAATGATGTTGAGTTCT+TGG + chr8.2:25490829-25490848 MS.gene56796:CDS 40.0%
GTTACCTGAATTGCTAGAGT+TGG - chr8.2:25489963-25489982 None:intergenic 40.0%
TATGGGAACCTTTCGAGATT+TGG - chr8.2:25490076-25490095 None:intergenic 40.0%
TCCATCATTTGCAACAACCT+CGG + chr8.2:25489807-25489826 MS.gene56796:CDS 40.0%
TGTTGTGGATATCACCTTGA+TGG - chr8.2:25489523-25489542 None:intergenic 40.0%
TTAAGTGACGGCATCTTCAT+TGG + chr8.2:25489987-25490006 MS.gene56796:CDS 40.0%
TTCGCTAGGTGGTAATATGT+TGG + chr8.2:25490628-25490647 MS.gene56796:CDS 40.0%
! CGTTGAAATGAAGGGAGTTT+TGG - chr8.2:25490104-25490123 None:intergenic 40.0%
! TGGTGCTTGAATTGTTGTTG+TGG - chr8.2:25489538-25489557 None:intergenic 40.0%
! TGGTTTCAAGTGGAAGGAAT+CGG - chr8.2:25489626-25489645 None:intergenic 40.0%
! TTTGGATGGTTTCAAGTGGA+AGG - chr8.2:25489632-25489651 None:intergenic 40.0%
!! GAGGTTCTAGAGTGTGAAAT+CGG + chr8.2:25490497-25490516 MS.gene56796:CDS 40.0%
AATGGTGTGAGGATGAAAGC+AGG + chr8.2:25490473-25490492 MS.gene56796:CDS 45.0%
ACCAAGGATGATTCCACCTT+TGG + chr8.2:25490418-25490437 MS.gene56796:CDS 45.0%
ACCGAGGTTGTTGCAAATGA+TGG - chr8.2:25489811-25489830 None:intergenic 45.0%
AGCACAAAACACAACTGTCG+CGG + chr8.2:25489851-25489870 MS.gene56796:CDS 45.0%
ATGGTGTGAGGATGAAAGCA+GGG + chr8.2:25490474-25490493 MS.gene56796:CDS 45.0%
ATTACAAGACGCCCTGCAAA+GGG - chr8.2:25489744-25489763 None:intergenic 45.0%
CCACTTGAAACCATCCAAAG+AGG + chr8.2:25489633-25489652 MS.gene56796:CDS 45.0%
CCTCTTTGGATGGTTTCAAG+TGG - chr8.2:25489636-25489655 None:intergenic 45.0%
GAAAAGAAGGCCTCTTTGGA+TGG - chr8.2:25489646-25489665 None:intergenic 45.0%
GAGGTGTGTAGTTGGATTGA+AGG - chr8.2:25489881-25489900 None:intergenic 45.0%
GGGAACCAACGTTGAAATGA+AGG - chr8.2:25490113-25490132 None:intergenic 45.0%
TCCAAAGGTGGAATCATCCT+TGG - chr8.2:25490422-25490441 None:intergenic 45.0%
TCTTGGTCCCAAATCTCGAA+AGG + chr8.2:25490065-25490084 MS.gene56796:CDS 45.0%
TTATTTCGGAAACGCGGTAG+TGG + chr8.2:25490448-25490467 MS.gene56796:CDS 45.0%
! AGCTTTCTTCTGAGGAGTCT+TGG - chr8.2:25490190-25490209 None:intergenic 45.0%
! GACAATTGGAGGTGTGTAGT+TGG - chr8.2:25489889-25489908 None:intergenic 45.0%
! GACAGTTGTGTTTTGTGCTG+TGG - chr8.2:25489850-25489869 None:intergenic 45.0%
! TGTGTGAAAGGGAAGCGTAT+TGG - chr8.2:25490152-25490171 None:intergenic 45.0%
! TTGTAGACGCCAAGTCGTTT+TGG + chr8.2:25490030-25490049 MS.gene56796:CDS 45.0%
!! CAGGTAACGGAGTTAAGTGA+CGG + chr8.2:25489975-25489994 MS.gene56796:CDS 45.0%
!! GAATGGGTTGGTGGAAAAGA+AGG - chr8.2:25489659-25489678 None:intergenic 45.0%
!! GTTTGTGGTGTCGTTGTGTT+GGG - chr8.2:25489769-25489788 None:intergenic 45.0%
!! TGTTTGTGGTGTCGTTGTGT+TGG - chr8.2:25489770-25489789 None:intergenic 45.0%
!! TTTGGTTAGGAATGGGTTGG+TGG - chr8.2:25489668-25489687 None:intergenic 45.0%
!!! GGTTTTGGTTAGGAATGGGT+TGG - chr8.2:25489671-25489690 None:intergenic 45.0%
ACACCAACGTTGTTCCGAAG+TGG + chr8.2:25490602-25490621 MS.gene56796:CDS 50.0%
CACCTAGCGAACTACCACTT+CGG - chr8.2:25490619-25490638 None:intergenic 50.0%
CATTCTTCCCACCCTTTGCA+GGG + chr8.2:25489730-25489749 MS.gene56796:CDS 50.0%
CTACCACTTCGGAACAACGT+TGG - chr8.2:25490608-25490627 None:intergenic 50.0%
GATTACAAGACGCCCTGCAA+AGG - chr8.2:25489745-25489764 None:intergenic 50.0%
GGTGGAAAAGAAGGCCTCTT+TGG - chr8.2:25489650-25489669 None:intergenic 50.0%
TCATTCTTCCCACCCTTTGC+AGG + chr8.2:25489729-25489748 MS.gene56796:CDS 50.0%
TGGTGTGAGGATGAAAGCAG+GGG + chr8.2:25490475-25490494 MS.gene56796:CDS 50.0%
TTCCGAAGTGGTAGTTCGCT+AGG + chr8.2:25490614-25490633 MS.gene56796:CDS 50.0%
! CGGTAGTGGTTAATGGTGTG+AGG + chr8.2:25490462-25490481 MS.gene56796:CDS 50.0%
! GGAAACGCGGTAGTGGTTAA+TGG + chr8.2:25490455-25490474 MS.gene56796:CDS 50.0%
! GTGTTTGCTGGTGCTGAAGA+AGG + chr8.2:25490758-25490777 MS.gene56796:CDS 50.0%
! TGGGAAGAATGAGAGTGTGG+TGG - chr8.2:25489721-25489740 None:intergenic 50.0%
! TTGTGTTTTGTGCTGTGGCG+TGG - chr8.2:25489845-25489864 None:intergenic 50.0%
!! TTTTGGTTGGGGAAAGCCTG+TGG + chr8.2:25490691-25490710 MS.gene56796:CDS 50.0%
AAGCCTGTGGCGTTTCGAAG+TGG + chr8.2:25490704-25490723 MS.gene56796:CDS 55.0%
ACTTGGCGTCTACAACAGCG+TGG - chr8.2:25490025-25490044 None:intergenic 55.0%
AGCCTGTGGCGTTTCGAAGT+GGG + chr8.2:25490705-25490724 MS.gene56796:CDS 55.0%
CGAAGTGGTAGTTCGCTAGG+TGG + chr8.2:25490617-25490636 MS.gene56796:CDS 55.0%
GGAAGGAATCGGAGATCCCA+TGG - chr8.2:25489615-25489634 None:intergenic 55.0%
GGGTGGGAAGAATGAGAGTG+TGG - chr8.2:25489724-25489743 None:intergenic 55.0%
TGTGAGGATGAAAGCAGGGG+AGG + chr8.2:25490478-25490497 MS.gene56796:CDS 55.0%
ACAAGACGCCCTGCAAAGGG+TGG - chr8.2:25489741-25489760 None:intergenic 60.0%
CAAGACGCCCTGCAAAGGGT+GGG - chr8.2:25489740-25489759 None:intergenic 60.0%
CTCCCACTTCGAAACGCCAC+AGG - chr8.2:25490710-25490729 None:intergenic 60.0%
GTGGCGTTTCGAAGTGGGAG+TGG + chr8.2:25490710-25490729 MS.gene56796:CDS 60.0%
! CGTGGACGAAGAGTGCACCG+AGG - chr8.2:25489827-25489846 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 25489513 25490868 25489513 ID=MS.gene56796
chr8.2 mRNA 25489513 25490868 25489513 ID=MS.gene56796.t1;Parent=MS.gene56796
chr8.2 exon 25489513 25490868 25489513 ID=MS.gene56796.t1.exon1;Parent=MS.gene56796.t1
chr8.2 CDS 25489513 25490868 25489513 ID=cds.MS.gene56796.t1;Parent=MS.gene56796.t1
Gene Sequence

>MS.gene56796

ATGGATTCCATCAAGGTGATATCCACAACAACAATTCAAGCACCAATTGACAACAATAATAATCTCAATTCAACTGATTATCAGAAAATTGATCTAACACCATGGGATCTCCGATTCCTTCCACTTGAAACCATCCAAAGAGGCCTTCTTTTCCACCAACCCATTCCTAACCAAAACCAAATCAACCATCTCAAACAAACACTTTCCACCACACTCTCATTCTTCCCACCCTTTGCAGGGCGTCTTGTAATCACCCAACACAACGACACCACAAACAACAACAACACATCATGTTCCATCATTTGCAACAACCTCGGTGCACTCTTCGTCCACGCCACAGCACAAAACACAACTGTCGCGGACATCCTTCAATCCAACTACACACCTCCAATTGTCCACTCATTTTTTACACTTAACGGTGTTAAAAACTACGAAGCTACGTCAAAACCAACTCTAGCAATTCAGGTAACGGAGTTAAGTGACGGCATCTTCATTGGCTTCACATGCAACCACGCTGTTGTAGACGCCAAGTCGTTTTGGCATTTCGTTAATTCTTGGTCCCAAATCTCGAAAGGTTCCCATAAAATACCAAAACTCCCTTCATTTCAACGTTGGTTCCCAAACGATATTGAAATTCCAATACGCTTCCCTTTCACACAAATACAATCACAAAACCAAGACTCCTCAGAAGAAAGCTTAGAATCCAATTTAGCTGAAAGAATCTTCCATTTCACAAAGGAGAAAATCGCGGAACTGAAATCAAAAGCAAACGCAGAAGCCGAAACAGATAAATCTAAAATATCTTCGCTTCAAGTACTATTATCTCATGTTTGGCGTTCTGTTGTTCGTTGCAAAAAACACGATCCACAAGAAGATGTTTTCTATGTGTTGATGATAGATGTTAGACCAAGGATGATTCCACCTTTGGAAGAAGATTATTTCGGAAACGCGGTAGTGGTTAATGGTGTGAGGATGAAAGCAGGGGAGGTTCTAGAGTGTGAAATCGGAAAAGTTGCTTTAGAGATAAACAAGATGATTTCATTACATTCTGCTGAGAAAATAAGGAACCATTACGAATCTTGGTTGAGAACACCAACGTTGTTCCGAAGTGGTAGTTCGCTAGGTGGTAATATGTTGGCAATGAGCAGTTCTCCGAGATTTGATGTTTTTGGCAATGATTTTGGTTGGGGAAAGCCTGTGGCGTTTCGAAGTGGGAGTGGTAATAAAAATAATGGAAAAATTAGTGTGTTTGCTGGTGCTGAAGAAGGTAGTATAGATGTTGAAATGTGTCTTTCTTATGAGATTTTGGAGGCTTTGGGAAATGATGTTGAGTTCTTGGTTCCTAGTTCTAAGTAA
Protein sequence

>MS.gene56796.t1

MDSIKVISTTTIQAPIDNNNNLNSTDYQKIDLTPWDLRFLPLETIQRGLLFHQPIPNQNQINHLKQTLSTTLSFFPPFAGRLVITQHNDTTNNNNTSCSIICNNLGALFVHATAQNTTVADILQSNYTPPIVHSFFTLNGVKNYEATSKPTLAIQVTELSDGIFIGFTCNHAVVDAKSFWHFVNSWSQISKGSHKIPKLPSFQRWFPNDIEIPIRFPFTQIQSQNQDSSEESLESNLAERIFHFTKEKIAELKSKANAEAETDKSKISSLQVLLSHVWRSVVRCKKHDPQEDVFYVLMIDVRPRMIPPLEEDYFGNAVVVNGVRMKAGEVLECEIGKVALEINKMISLHSAEKIRNHYESWLRTPTLFRSGSSLGGNMLAMSSSPRFDVFGNDFGWGKPVAFRSGSGNKNNGKISVFAGAEEGSIDVEMCLSYEILEALGNDVEFLVPSSK