AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32261

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32261.t1 MTR_0190s0020 93.905 443 26 1 1 442 1 443 0.0 867
MS.gene32261.t1 MTR_8g041770 71.493 442 124 2 1 441 1 441 0.0 639
MS.gene32261.t1 MTR_4g022800 59.956 452 160 6 1 441 1 442 0.0 539
MS.gene32261.t1 MTR_4g022670 58.758 451 166 6 1 441 1 441 0.0 531
MS.gene32261.t1 MTR_4g022720 60.000 445 164 6 1 441 1 435 0.0 530
MS.gene32261.t1 MTR_3g088420 60.225 445 161 6 1 441 1 433 0.0 527
MS.gene32261.t1 MTR_7g026860 58.186 452 164 7 1 441 1 438 0.0 523
MS.gene32261.t1 MTR_4g022825 57.965 452 167 7 1 441 1 440 0.0 521
MS.gene32261.t1 MTR_3g026310 57.205 458 169 6 1 441 1 448 3.43e-180 511
MS.gene32261.t1 MTR_4g094835 58.036 448 171 8 1 437 1 442 2.93e-179 508
MS.gene32261.t1 MTR_4g094842 55.902 449 185 5 1 439 1 446 5.26e-173 492
MS.gene32261.t1 MTR_4g094838 55.134 448 167 8 4 437 1 428 2.16e-166 475
MS.gene32261.t1 MTR_4g094832 54.695 426 172 7 28 437 6 426 9.88e-160 458
MS.gene32261.t1 MTR_4g022760 52.000 450 154 6 1 441 1 397 4.27e-155 445
MS.gene32261.t1 MTR_4g094828 50.658 456 204 9 1 441 1 450 1.27e-153 443
MS.gene32261.t1 MTR_8g079710 51.577 444 195 9 1 432 1 436 1.22e-152 440
MS.gene32261.t1 MTR_4g022290 50.441 454 199 10 1 439 1 443 5.04e-148 429
MS.gene32261.t1 MTR_6g016815 45.946 444 160 10 1 441 15 381 1.52e-117 348
MS.gene32261.t1 MTR_3g101900 39.529 425 241 9 28 441 29 448 3.04e-94 291
MS.gene32261.t1 MTR_3g101930 39.229 441 246 10 17 440 19 454 3.66e-92 286
MS.gene32261.t1 MTR_3g102010 40.047 427 239 9 28 441 29 451 2.38e-90 281
MS.gene32261.t1 MTR_8g040760 64.486 214 73 2 1 213 1 212 6.23e-88 267
MS.gene32261.t1 MTR_3g102020 38.785 428 244 9 28 441 28 451 2.94e-87 273
MS.gene32261.t1 MTR_3g101970 38.061 423 238 9 28 440 28 436 1.12e-86 271
MS.gene32261.t1 MTR_8g040780 58.525 217 87 2 223 436 11 227 3.06e-86 264
MS.gene32261.t1 MTR_3g101960 38.389 422 243 10 28 436 28 445 6.80e-85 268
MS.gene32261.t1 MTR_3g070170 31.470 483 266 9 2 441 10 470 1.34e-72 236
MS.gene32261.t1 MTR_4g015180 33.730 504 250 15 2 441 4 487 3.80e-66 219
MS.gene32261.t1 MTR_5g081960 32.068 474 253 9 22 437 20 482 1.53e-65 218
MS.gene32261.t1 MTR_7g026850 61.212 165 60 2 24 188 3 163 2.83e-64 205
MS.gene32261.t1 MTR_4g094825 51.500 200 87 4 17 209 80 276 2.29e-57 194
MS.gene32261.t1 MTR_4g094825 52.980 151 59 2 292 441 252 391 6.20e-47 166
MS.gene32261.t1 MTR_7g026770 51.309 191 70 3 149 337 132 301 3.35e-55 186
MS.gene32261.t1 MTR_6g034495 71.774 124 30 2 73 191 44 167 4.09e-53 176
MS.gene32261.t1 MTR_6g045553 30.804 448 262 10 28 437 34 471 4.10e-51 179
MS.gene32261.t1 MTR_6g034485 55.556 126 55 1 194 319 1 125 7.44e-47 157
MS.gene32261.t1 MTR_3g102030 33.993 303 165 7 98 395 48 320 1.40e-46 163
MS.gene32261.t1 MTR_7g026830 64.286 84 30 0 90 173 14 97 6.21e-33 120
MS.gene32261.t1 MTR_4g007540 25.000 412 272 13 41 432 34 428 1.19e-31 125
MS.gene32261.t1 MTR_2g105330 26.302 384 249 12 63 428 56 423 1.74e-30 122
MS.gene32261.t1 MTR_2g064495 26.302 384 249 12 63 428 56 423 2.66e-30 122
MS.gene32261.t1 MTR_8g096530 31.287 342 207 12 61 390 57 382 6.40e-30 121
MS.gene32261.t1 MTR_8g075610 26.873 387 246 14 63 431 47 414 6.56e-30 120
MS.gene32261.t1 MTR_3g098980 28.158 380 240 14 63 428 66 426 1.79e-29 119
MS.gene32261.t1 MTR_3g026950 27.470 415 251 16 47 426 38 437 2.09e-29 119
MS.gene32261.t1 MTR_3g049150 27.273 418 229 16 60 431 52 440 8.02e-26 109
MS.gene32261.t1 MTR_7g026780 49.550 111 31 2 331 441 26 111 8.87e-25 98.6
MS.gene32261.t1 MTR_4g097090 27.179 390 240 16 63 422 65 440 7.32e-24 103
MS.gene32261.t1 MTR_4g127650 25.714 385 251 13 63 422 66 440 1.01e-23 103
MS.gene32261.t1 MTR_4g013050 25.320 391 236 12 61 432 51 404 3.47e-22 98.6
MS.gene32261.t1 MTR_4g022780 36.022 186 78 6 201 375 2 157 3.67e-22 93.6
MS.gene32261.t1 MTR_7g084940 28.696 345 212 12 61 390 67 392 5.95e-22 98.2
MS.gene32261.t1 MTR_7g027175 25.128 390 258 11 58 432 63 433 8.23e-22 97.8
MS.gene32261.t1 MTR_7g014330 28.058 417 226 17 26 394 23 413 8.44e-22 97.8
MS.gene32261.t1 MTR_7g026800 38.312 154 64 4 224 375 32 156 2.07e-21 92.0
MS.gene32261.t1 MTR_1g040175 25.191 393 255 17 58 430 59 432 3.25e-21 95.9
MS.gene32261.t1 MTR_7g026870 68.519 54 17 0 297 350 98 151 4.14e-21 89.7
MS.gene32261.t1 MTR_7g086000 25.799 407 248 15 63 431 60 450 5.43e-21 95.5
MS.gene32261.t1 MTR_7g014250 27.338 417 229 17 26 394 23 413 1.03e-20 94.7
MS.gene32261.t1 MTR_3g086020 26.005 423 275 13 28 441 22 415 5.69e-20 92.0
MS.gene32261.t1 MTR_2g015430 26.184 359 229 11 48 393 51 386 1.23e-19 91.3
MS.gene32261.t1 MTR_6g015315 26.984 315 188 10 146 429 140 443 2.25e-19 90.5
MS.gene32261.t1 MTR_2g089755 26.650 409 245 17 29 394 37 433 3.40e-19 90.1
MS.gene32261.t1 MTR_7g014230 27.517 447 234 22 26 414 25 439 1.06e-18 88.6
MS.gene32261.t1 MTR_1g053315 25.797 345 228 15 63 391 78 410 1.94e-18 87.8
MS.gene32261.t1 MTR_8g079980 25.000 392 224 15 55 414 51 404 2.17e-18 87.4
MS.gene32261.t1 MTR_2g089765 24.947 469 290 14 3 425 15 467 5.48e-18 86.7
MS.gene32261.t1 MTR_2g089650 25.581 430 271 14 26 418 26 443 5.56e-18 86.3
MS.gene32261.t1 MTR_6g015320 27.273 308 193 10 146 428 143 444 6.30e-18 86.3
MS.gene32261.t1 MTR_1g040195 26.053 380 224 15 77 428 55 405 8.03e-18 85.5
MS.gene32261.t1 MTR_7g114520 24.000 375 259 12 46 407 49 410 1.01e-17 85.5
MS.gene32261.t1 MTR_3g087640 25.800 469 287 20 5 428 10 462 2.46e-17 84.3
MS.gene32261.t1 MTR_1g081660 23.902 410 236 16 29 400 31 402 3.08e-17 84.0
MS.gene32261.t1 MTR_7g015860 25.729 377 205 16 63 391 64 413 4.43e-17 83.6
MS.gene32261.t1 MTR_7g114550 23.185 427 275 16 30 425 24 428 5.73e-17 83.2
MS.gene32261.t1 MTR_3g049140 27.083 240 148 9 211 431 6 237 1.42e-16 79.7
MS.gene32261.t1 MTR_7g085930 24.814 403 256 12 60 426 56 447 7.76e-16 79.7
MS.gene32261.t1 MTR_3g465980 25.830 271 184 6 63 323 68 331 1.06e-15 79.3
MS.gene32261.t1 MTR_5g026320 24.891 458 292 20 4 436 18 448 1.19e-15 79.3
MS.gene32261.t1 MTR_1g040320 23.810 399 239 15 47 430 48 396 1.50e-15 78.6
MS.gene32261.t1 MTR_3g112180 25.258 388 239 15 25 389 29 388 2.91e-15 78.2
MS.gene32261.t1 MTR_3g465990 26.471 272 181 8 63 323 68 331 3.12e-15 77.8
MS.gene32261.t1 MTR_4g055170 25.806 310 207 8 52 350 58 355 7.76e-15 76.6
MS.gene32261.t1 MTR_7g084910 28.571 259 165 8 61 307 68 318 2.11e-14 75.1
MS.gene32261.t1 MTR_2g089670 24.479 384 240 11 43 390 60 429 5.80e-14 73.9
MS.gene32261.t1 MTR_7g014360 29.104 268 157 11 146 394 90 343 7.77e-14 73.2
MS.gene32261.t1 MTR_7g085990 24.000 400 261 12 60 425 52 442 9.22e-14 73.6
MS.gene32261.t1 MTR_1g040315 24.344 419 242 19 30 428 23 386 1.51e-13 72.4
MS.gene32261.t1 MTR_7g026880 55.556 54 22 1 388 441 2 53 5.88e-13 63.5
MS.gene32261.t1 MTR_7g056087 23.282 262 183 4 77 323 72 330 6.63e-12 67.4
MS.gene32261.t1 MTR_2g089735 24.886 438 270 20 37 430 31 453 6.28e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32261.t1 AT5G67150 40.991 444 246 10 4 437 5 442 1.49e-106 323
MS.gene32261.t1 AT3G50280 37.668 446 264 9 1 438 1 440 5.97e-101 308
MS.gene32261.t1 AT3G50300 39.908 436 244 11 1 426 1 428 4.49e-100 306
MS.gene32261.t1 AT5G67160 40.141 426 234 9 22 441 22 432 1.56e-96 297
MS.gene32261.t1 AT3G50270 37.584 447 266 9 4 441 6 448 2.15e-95 294
MS.gene32261.t1 AT5G42830 38.584 438 248 11 18 441 19 449 2.16e-88 276
MS.gene32261.t1 AT5G38130 38.391 435 242 13 1 422 21 442 4.62e-85 268
MS.gene32261.t1 AT5G07860 38.372 430 237 10 28 440 30 448 4.95e-83 263
MS.gene32261.t1 AT5G07850 38.426 432 240 11 28 441 30 453 1.33e-79 254
MS.gene32261.t1 AT5G07870 36.508 441 244 10 28 441 30 461 2.07e-75 243
MS.gene32261.t1 AT5G01210 36.009 461 243 10 22 441 20 469 3.02e-74 240
MS.gene32261.t1 AT2G39980 33.951 486 256 11 9 441 7 480 2.07e-72 236
MS.gene32261.t1 AT3G50280 38.462 312 184 5 133 438 1 310 1.64e-71 228
MS.gene32261.t1 AT5G23940 32.230 453 281 10 4 437 3 448 1.32e-66 221
MS.gene32261.t1 AT3G50290 42.322 267 145 5 130 388 4 269 8.72e-55 184
MS.gene32261.t1 AT5G48930 26.667 405 260 12 41 425 34 421 5.24e-30 121
MS.gene32261.t1 AT3G48720 26.263 396 263 12 47 429 44 423 4.82e-28 115
MS.gene32261.t1 AT2G19070 26.988 415 263 14 47 432 42 445 3.36e-27 113
MS.gene32261.t1 AT5G41040 25.750 400 255 14 55 433 59 437 1.56e-24 105
MS.gene32261.t1 AT2G40230 26.923 390 257 12 20 395 16 391 2.00e-24 105
MS.gene32261.t1 AT5G41040 25.750 400 255 14 55 433 75 453 2.16e-24 105
MS.gene32261.t1 AT3G29590 25.056 447 301 13 2 428 6 438 7.87e-22 97.8
MS.gene32261.t1 AT1G28680 29.104 268 163 9 140 393 135 389 3.67e-21 95.9
MS.gene32261.t1 AT3G03480 25.635 433 262 17 30 428 33 439 5.43e-20 92.4
MS.gene32261.t1 AT1G28680 30.890 191 122 5 140 323 135 322 9.08e-20 91.3
MS.gene32261.t1 AT3G26040 25.682 440 271 15 28 441 20 429 3.58e-18 87.0
MS.gene32261.t1 AT5G23970 24.289 457 281 16 4 437 3 417 9.85e-18 85.5
MS.gene32261.t1 AT1G03390 25.750 400 260 17 47 423 59 444 2.67e-17 84.3
MS.gene32261.t1 AT1G24420 24.603 378 253 13 73 441 75 429 9.77e-17 82.4
MS.gene32261.t1 AT1G24420 24.603 378 253 13 73 441 75 429 9.77e-17 82.4
MS.gene32261.t1 AT3G30280 23.810 441 283 18 25 441 25 436 3.05e-16 80.9
MS.gene32261.t1 AT5G39090 27.541 305 177 13 143 420 140 427 4.24e-16 80.5
MS.gene32261.t1 AT5G61160 24.359 312 198 10 143 428 139 438 4.39e-16 80.5
MS.gene32261.t1 AT5G57840 24.561 456 306 18 4 439 3 440 5.12e-16 80.1
MS.gene32261.t1 AT1G78990 25.876 371 222 15 48 391 52 396 9.92e-16 79.3
MS.gene32261.t1 AT5G07080 24.643 280 170 10 64 321 71 331 1.80e-15 78.6
MS.gene32261.t1 AT4G15390 22.706 436 287 15 25 437 27 435 2.43e-15 78.2
MS.gene32261.t1 AT4G15390 22.706 436 287 15 25 437 27 435 2.43e-15 78.2
MS.gene32261.t1 AT1G32910 26.593 361 211 15 61 391 63 399 4.02e-15 77.8
MS.gene32261.t1 AT1G65450 24.571 350 225 15 61 390 70 400 4.22e-14 74.3
MS.gene32261.t1 AT2G23510 23.677 359 219 14 63 393 69 400 8.91e-14 73.6
MS.gene32261.t1 AT5G47950 22.770 426 287 17 26 441 26 419 1.10e-13 73.2
MS.gene32261.t1 AT5G16410 25.193 389 221 19 63 415 91 445 3.73e-13 71.6
MS.gene32261.t1 AT3G47170 22.184 293 204 10 50 321 55 344 5.49e-13 70.9
MS.gene32261.t1 AT2G25150 24.823 282 176 12 147 407 148 414 6.52e-12 67.8
MS.gene32261.t1 AT1G31490 22.959 392 269 14 60 436 59 432 3.15e-11 65.5

Find 95 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCTTGGTTTGATGTTTA+TGG 0.170784 8.3:+48695304 MS.gene32261:CDS
TAGAAATGATCCTGAGTTTA+TGG 0.174155 8.3:+48695471 MS.gene32261:CDS
TAGAAGAAAGAGAGTCTTTA+AGG 0.197250 8.3:-48694367 None:intergenic
ATGTCTTCCTTGTGAAATTT+TGG 0.200982 8.3:+48695441 MS.gene32261:CDS
CCGTTGCCTGAGGATTATTT+TGG 0.234642 8.3:+48695076 MS.gene32261:CDS
GCGAAATTCGTGCTGGATAT+TGG 0.263514 8.3:+48695031 MS.gene32261:CDS
AATAGTGATTCATTGGTAAT+TGG 0.267941 8.3:+48695277 MS.gene32261:CDS
GAGAGTATTTCATTTCAAAA+AGG 0.290139 8.3:+48694877 MS.gene32261:CDS
GATGTTTATGGTAATGATTT+TGG 0.307637 8.3:+48695316 MS.gene32261:CDS
ACGATCAAATGTTGGAAGTT+TGG 0.316033 8.3:-48694756 None:intergenic
AAAATTCAAAGCAAACTTAG+AGG 0.330646 8.3:+48694916 MS.gene32261:CDS
TGGGCTTGGTAAGGGCTCTT+TGG 0.337089 8.3:+48695156 MS.gene32261:CDS
GATCTTCAATTCCTCCCATT+TGG 0.358724 8.3:+48694278 MS.gene32261:CDS
TATTGTGAAAGGAAATCTAA+TGG 0.359609 8.3:-48694804 None:intergenic
TTGAAATGAAATACTCTCTC+TGG 0.360829 8.3:-48694872 None:intergenic
GAAGATCCCATGGGGTTAGA+TGG 0.378133 8.3:-48694262 None:intergenic
GTTAACCACGTGATTGTTGA+TGG 0.384791 8.3:+48694671 MS.gene32261:CDS
GGTGATAAAGAAGCCCCTTT+TGG 0.389061 8.3:-48694307 None:intergenic
CGAATTTCGCTTCTGCTTGT+GGG 0.396357 8.3:-48695018 None:intergenic
AAAGAGAGTCTTTAAGGTGT+TGG 0.398134 8.3:-48694361 None:intergenic
ACGAATTTCGCTTCTGCTTG+TGG 0.401202 8.3:-48695019 None:intergenic
CCCTTTGTTGTTGCACAAGA+TGG 0.408570 8.3:-48694462 None:intergenic
CATTAAAGCCGGTGACTTGT+TGG 0.413794 8.3:+48695126 MS.gene32261:CDS
TGGTAATTGGTGGTTCTCCT+TGG 0.420077 8.3:+48695290 MS.gene32261:CDS
CAACTTCTCATCTGTGTGAA+AGG 0.425928 8.3:-48695197 None:intergenic
TTGTTGATGGTACGTCGTTT+TGG 0.426327 8.3:+48694684 MS.gene32261:CDS
GGAAGACGGTGGGCTTGGTA+AGG 0.427310 8.3:+48695147 MS.gene32261:CDS
AATTACCAATGAATCACTAT+TGG 0.429435 8.3:-48695275 None:intergenic
CCCATTTGGATTCAACCAAA+AGG 0.433362 8.3:+48694292 MS.gene32261:CDS
ATTGTGAAAGGAAATCTAAT+GGG 0.441170 8.3:-48694803 None:intergenic
CCATTTGGATTCAACCAAAA+GGG 0.443246 8.3:+48694293 MS.gene32261:CDS
TTAGTCGACGGCATCTTCAT+TGG 0.444242 8.3:+48694641 MS.gene32261:CDS
TTTGGCATTTCATCAATTCA+TGG 0.452731 8.3:+48694702 MS.gene32261:CDS
TATGGCCAATAGTGATTCAT+TGG 0.457840 8.3:+48695270 MS.gene32261:CDS
CGTTACACAAGCCGATTAGT+AGG 0.472331 8.3:-48695503 None:intergenic
AGTTGTGGAGAGGACTTTGA+TGG 0.477018 8.3:-48694189 None:intergenic
AACTTCCAACATTTGATCGT+TGG 0.480962 8.3:+48694759 MS.gene32261:CDS
AGTGATTCATTGGTAATTGG+TGG 0.482373 8.3:+48695280 MS.gene32261:CDS
CCATCTTGTGCAACAACAAA+GGG 0.484933 8.3:+48694462 MS.gene32261:CDS
TTGGCATTTCATCAATTCAT+GGG 0.489109 8.3:+48694703 MS.gene32261:CDS
AATGAGGATAATTATGAAGA+AGG 0.489922 8.3:+48694836 MS.gene32261:CDS
CACAAGGAAGACATACTTCA+AGG 0.496352 8.3:-48695432 None:intergenic
TGAATAGGAGGATGATAAGT+GGG 0.498813 8.3:-48694539 None:intergenic
CACAATAGAGTCACGAAATG+AGG 0.502975 8.3:+48694820 MS.gene32261:CDS
GTGAATAGGAGGATGATAAG+TGG 0.505672 8.3:-48694540 None:intergenic
CGTCGACTAACTCTGTTACT+TGG 0.506191 8.3:-48694628 None:intergenic
TTCAAAGCAAACTTAGAGGT+TGG 0.511060 8.3:+48694920 MS.gene32261:CDS
TGCTGCATGTATTGTAGTTG+TGG 0.511804 8.3:-48694204 None:intergenic
TTGTTCTATCTAATTCGAAA+GGG 0.519195 8.3:-48694331 None:intergenic
TTGTTGGAAGACGGTGGGCT+TGG 0.520935 8.3:+48695142 MS.gene32261:CDS
ATGAGTGAAAATTGCTTGCA+AGG 0.523442 8.3:-48694960 None:intergenic
TTTCGTGACTCTATTGTGAA+AGG 0.529927 8.3:-48694815 None:intergenic
TTTGTTCTATCTAATTCGAA+AGG 0.534586 8.3:-48694332 None:intergenic
GAAAACCAACGATCAAATGT+TGG 0.535798 8.3:-48694764 None:intergenic
GAAGACGGTGGGCTTGGTAA+GGG 0.539527 8.3:+48695148 MS.gene32261:CDS
TTCTATCTAATTCGAAAGGG+TGG 0.542163 8.3:-48694328 None:intergenic
GTGACTTGTTGGAAGACGGT+GGG 0.548009 8.3:+48695137 MS.gene32261:CDS
ACCGTCTTCCAACAAGTCAC+CGG 0.551339 8.3:-48695134 None:intergenic
AGCAGAAGCGAAATTCGTGC+TGG 0.555369 8.3:+48695024 MS.gene32261:CDS
CATTTGGATTCAACCAAAAG+GGG 0.560740 8.3:+48694294 MS.gene32261:CDS
ATACCAGCGGTGACAAAACT+CGG 0.561514 8.3:-48695250 None:intergenic
GGGAGGAATTGAAGATCCCA+TGG 0.563488 8.3:-48694272 None:intergenic
GGAAAGATTTATGTGTATGC+GGG 0.563929 8.3:+48695388 MS.gene32261:CDS
GCATTACCAAAATAATCCTC+AGG 0.566644 8.3:-48695082 None:intergenic
TGTGTTGTTAGCATTAAAGC+CGG 0.567065 8.3:+48695115 MS.gene32261:CDS
TATCTTGAGACGACCTGTGA+AGG 0.574633 8.3:-48694408 None:intergenic
CCAAAATAATCCTCAGGCAA+CGG 0.577247 8.3:-48695076 None:intergenic
TCCATCTTGTGCAACAACAA+AGG 0.578209 8.3:+48694461 MS.gene32261:CDS
GGCAACGGAGGAATCAACCT+TGG 0.584288 8.3:-48695061 None:intergenic
ATAAGTGGGTTTAACGATGT+CGG 0.584805 8.3:-48694525 None:intergenic
GCCGGTGACTTGTTGGAAGA+CGG 0.588361 8.3:+48695133 MS.gene32261:CDS
GTTGATTCCTCCGTTGCCTG+AGG 0.590687 8.3:+48695066 MS.gene32261:CDS
GGTGACTTGTTGGAAGACGG+TGG 0.597827 8.3:+48695136 MS.gene32261:CDS
GTGTATGCGGGTGTAGAAGA+TGG 0.601730 8.3:+48695400 MS.gene32261:CDS
ATACAATCCATCTAACCCCA+TGG 0.603547 8.3:+48694255 MS.gene32261:CDS
GCTACGGCATGAACAAAGAG+TGG 0.606907 8.3:-48694485 None:intergenic
TACAATCCATCTAACCCCAT+GGG 0.610598 8.3:+48694256 MS.gene32261:CDS
TTTGGTTGAATCCAAATGGG+AGG 0.611475 8.3:-48694289 None:intergenic
GTTACTTGGACTGCAAGTAA+CGG 0.617187 8.3:-48694614 None:intergenic
TGGAAAGATTTATGTGTATG+CGG 0.617965 8.3:+48695387 MS.gene32261:CDS
ATCTTGAGACGACCTGTGAA+GGG 0.618770 8.3:-48694407 None:intergenic
TCATGGGCTGAAATCTCGAA+AGG 0.621563 8.3:+48694719 MS.gene32261:CDS
GACACAACATCCATAAACTC+AGG 0.640217 8.3:-48695481 None:intergenic
GGAGGAATTGAAGATCCCAT+GGG 0.641441 8.3:-48694271 None:intergenic
AAATAATCCTCAGGCAACGG+AGG 0.648191 8.3:-48695073 None:intergenic
ATCACTATTGGCCATACCAG+CGG 0.651003 8.3:-48695263 None:intergenic
ATTGCCTCCAAAATTTCACA+AGG 0.652126 8.3:-48695448 None:intergenic
CAAGTAACAGAGTTAGTCGA+CGG 0.663446 8.3:+48694629 MS.gene32261:CDS
GGGTGTAGAAGATGGTGGTA+TGG 0.663876 8.3:+48695408 MS.gene32261:CDS
TTACCATAAACATCAAACCA+AGG 0.678732 8.3:-48695307 None:intergenic
CATGTATTGTAGTTGTGGAG+AGG 0.679095 8.3:-48694199 None:intergenic
TGGATATTGGAATTAGACCA+AGG 0.680206 8.3:+48695044 MS.gene32261:CDS
TATGCGGGTGTAGAAGATGG+TGG 0.703135 8.3:+48695403 MS.gene32261:CDS
ACGTACCATCAACAATCACG+TGG 0.741961 8.3:-48694676 None:intergenic
GAGGAATTGAAGATCCCATG+GGG 0.782196 8.3:-48694270 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAAAATCACTATGAAAAT+TGG + chr8.3:48695218-48695237 MS.gene32261:CDS 20.0%
! AAAATTCAAAGCAAACTTAG+AGG + chr8.3:48694916-48694935 MS.gene32261:CDS 25.0%
! AATGAGGATAATTATGAAGA+AGG + chr8.3:48694836-48694855 MS.gene32261:CDS 25.0%
! AATGGAGTTAAAAACTATGA+AGG + chr8.3:48694581-48694600 MS.gene32261:CDS 25.0%
! AATTACCAATGAATCACTAT+TGG - chr8.3:48695278-48695297 None:intergenic 25.0%
! ATTGTGAAAGGAAATCTAAT+GGG - chr8.3:48694806-48694825 None:intergenic 25.0%
! TATTGTGAAAGGAAATCTAA+TGG - chr8.3:48694807-48694826 None:intergenic 25.0%
! TTGTTCTATCTAATTCGAAA+GGG - chr8.3:48694334-48694353 None:intergenic 25.0%
! TTTGTTCTATCTAATTCGAA+AGG - chr8.3:48694335-48694354 None:intergenic 25.0%
!! AAGCAATTTTCACTCATATT+TGG + chr8.3:48694966-48694985 MS.gene32261:CDS 25.0%
!! AATAGTGATTCATTGGTAAT+TGG + chr8.3:48695277-48695296 MS.gene32261:CDS 25.0%
!!! AACACTAATGTTTTTTGCTA+CGG - chr8.3:48694504-48694523 None:intergenic 25.0%
!!! GAGAGTATTTCATTTCAAAA+AGG + chr8.3:48694877-48694896 MS.gene32261:CDS 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr8.3:48695316-48695335 MS.gene32261:CDS 25.0%
!!! TTATGGTAATGATTTTGGTT+GGG + chr8.3:48695321-48695340 MS.gene32261:CDS 25.0%
!!! TTTATGGTAATGATTTTGGT+TGG + chr8.3:48695320-48695339 MS.gene32261:CDS 25.0%
TAGAAATGATCCTGAGTTTA+TGG + chr8.3:48695471-48695490 MS.gene32261:CDS 30.0%
TAGAAGAAAGAGAGTCTTTA+AGG - chr8.3:48694370-48694389 None:intergenic 30.0%
TGGAAAGATTTATGTGTATG+CGG + chr8.3:48695387-48695406 MS.gene32261:CDS 30.0%
TTACCATAAACATCAAACCA+AGG - chr8.3:48695310-48695329 None:intergenic 30.0%
TTGAAATGAAATACTCTCTC+TGG - chr8.3:48694875-48694894 None:intergenic 30.0%
TTGGCATTTCATCAATTCAT+GGG + chr8.3:48694703-48694722 MS.gene32261:CDS 30.0%
TTTGGCATTTCATCAATTCA+TGG + chr8.3:48694702-48694721 MS.gene32261:CDS 30.0%
! ATGTCTTCCTTGTGAAATTT+TGG + chr8.3:48695441-48695460 MS.gene32261:CDS 30.0%
! CATTTAGAGCAAAAAATGAG+TGG - chr8.3:48694565-48694584 None:intergenic 30.0%
!!! CACTCATTTTTTGCTCTAAA+TGG + chr8.3:48694563-48694582 MS.gene32261:CDS 30.0%
!!! CTTCTACCCTTGATATTTTT+TGG + chr8.3:48694384-48694403 MS.gene32261:CDS 30.0%
!!! TATGGTAATGATTTTGGTTG+GGG + chr8.3:48695322-48695341 MS.gene32261:CDS 30.0%
!!! TTTGATCGTTGGTTTTCAAA+AGG + chr8.3:48694770-48694789 MS.gene32261:CDS 30.0%
AAAAATGAGTGGTGAATAGG+AGG - chr8.3:48694554-48694573 None:intergenic 35.0%
AACTTCCAACATTTGATCGT+TGG + chr8.3:48694759-48694778 MS.gene32261:CDS 35.0%
ACGATCAAATGTTGGAAGTT+TGG - chr8.3:48694759-48694778 None:intergenic 35.0%
ATGAGTGAAAATTGCTTGCA+AGG - chr8.3:48694963-48694982 None:intergenic 35.0%
ATTGCCTCCAAAATTTCACA+AGG - chr8.3:48695451-48695470 None:intergenic 35.0%
CATTTGGATTCAACCAAAAG+GGG + chr8.3:48694294-48694313 MS.gene32261:CDS 35.0%
CCATTTGGATTCAACCAAAA+GGG + chr8.3:48694293-48694312 MS.gene32261:CDS 35.0%
GAAAACCAACGATCAAATGT+TGG - chr8.3:48694767-48694786 None:intergenic 35.0%
GAAGGGCCAAAAAATATCAA+GGG - chr8.3:48694393-48694412 None:intergenic 35.0%
GCAAAAAATGAGTGGTGAAT+AGG - chr8.3:48694557-48694576 None:intergenic 35.0%
GCATTACCAAAATAATCCTC+AGG - chr8.3:48695085-48695104 None:intergenic 35.0%
GGAAAGATTTATGTGTATGC+GGG + chr8.3:48695388-48695407 MS.gene32261:CDS 35.0%
TATGGCCAATAGTGATTCAT+TGG + chr8.3:48695270-48695289 MS.gene32261:CDS 35.0%
TGAAGGGCCAAAAAATATCA+AGG - chr8.3:48694394-48694413 None:intergenic 35.0%
TGAATAGGAGGATGATAAGT+GGG - chr8.3:48694542-48694561 None:intergenic 35.0%
TGGATATTGGAATTAGACCA+AGG + chr8.3:48695044-48695063 MS.gene32261:CDS 35.0%
TTCAAAGCAAACTTAGAGGT+TGG + chr8.3:48694920-48694939 MS.gene32261:CDS 35.0%
TTCTATCTAATTCGAAAGGG+TGG - chr8.3:48694331-48694350 None:intergenic 35.0%
TTTCGTGACTCTATTGTGAA+AGG - chr8.3:48694818-48694837 None:intergenic 35.0%
! AGTGATTCATTGGTAATTGG+TGG + chr8.3:48695280-48695299 MS.gene32261:CDS 35.0%
! ATAAGTGGGTTTAACGATGT+CGG - chr8.3:48694528-48694547 None:intergenic 35.0%
! TCTCCTTGGTTTGATGTTTA+TGG + chr8.3:48695304-48695323 MS.gene32261:CDS 35.0%
! TCTTCCTTGTGAAATTTTGG+AGG + chr8.3:48695444-48695463 MS.gene32261:CDS 35.0%
! TGTGTTGTTAGCATTAAAGC+CGG + chr8.3:48695115-48695134 MS.gene32261:CDS 35.0%
!! AAAGAGAGTCTTTAAGGTGT+TGG - chr8.3:48694364-48694383 None:intergenic 35.0%
!! CCTTTTGGTTGAATCCAAAT+GGG - chr8.3:48694295-48694314 None:intergenic 35.0%
!!! ATGGTAATGATTTTGGTTGG+GGG + chr8.3:48695323-48695342 MS.gene32261:CDS 35.0%
ATACAATCCATCTAACCCCA+TGG + chr8.3:48694255-48694274 MS.gene32261:CDS 40.0%
CAACTTCTCATCTGTGTGAA+AGG - chr8.3:48695200-48695219 None:intergenic 40.0%
CAAGTAACAGAGTTAGTCGA+CGG + chr8.3:48694629-48694648 MS.gene32261:CDS 40.0%
CACAAGGAAGACATACTTCA+AGG - chr8.3:48695435-48695454 None:intergenic 40.0%
CACAATAGAGTCACGAAATG+AGG + chr8.3:48694820-48694839 MS.gene32261:CDS 40.0%
CATGTATTGTAGTTGTGGAG+AGG - chr8.3:48694202-48694221 None:intergenic 40.0%
CCAAAATAATCCTCAGGCAA+CGG - chr8.3:48695079-48695098 None:intergenic 40.0%
CCATCTTGTGCAACAACAAA+GGG + chr8.3:48694462-48694481 MS.gene32261:CDS 40.0%
CCCATTTGGATTCAACCAAA+AGG + chr8.3:48694292-48694311 MS.gene32261:CDS 40.0%
GACACAACATCCATAAACTC+AGG - chr8.3:48695484-48695503 None:intergenic 40.0%
GATCTTCAATTCCTCCCATT+TGG + chr8.3:48694278-48694297 MS.gene32261:CDS 40.0%
GTGAATAGGAGGATGATAAG+TGG - chr8.3:48694543-48694562 None:intergenic 40.0%
GTTAACCACGTGATTGTTGA+TGG + chr8.3:48694671-48694690 MS.gene32261:CDS 40.0%
GTTACTTGGACTGCAAGTAA+CGG - chr8.3:48694617-48694636 None:intergenic 40.0%
TACAATCCATCTAACCCCAT+GGG + chr8.3:48694256-48694275 MS.gene32261:CDS 40.0%
TCCATCTTGTGCAACAACAA+AGG + chr8.3:48694461-48694480 MS.gene32261:CDS 40.0%
TGCTGCATGTATTGTAGTTG+TGG - chr8.3:48694207-48694226 None:intergenic 40.0%
TTTGGTTGAATCCAAATGGG+AGG - chr8.3:48694292-48694311 None:intergenic 40.0%
!! CCCTTTTGGTTGAATCCAAA+TGG - chr8.3:48694296-48694315 None:intergenic 40.0%
!!! GATATTTTTTGGCCCTTCAC+AGG + chr8.3:48694395-48694414 MS.gene32261:CDS 40.0%
!!! TTGTTGATGGTACGTCGTTT+TGG + chr8.3:48694684-48694703 MS.gene32261:CDS 40.0%
AAATAATCCTCAGGCAACGG+AGG - chr8.3:48695076-48695095 None:intergenic 45.0%
ACGAATTTCGCTTCTGCTTG+TGG - chr8.3:48695022-48695041 None:intergenic 45.0%
ACGTACCATCAACAATCACG+TGG - chr8.3:48694679-48694698 None:intergenic 45.0%
AGTTGTGGAGAGGACTTTGA+TGG - chr8.3:48694192-48694211 None:intergenic 45.0%
ATACCAGCGGTGACAAAACT+CGG - chr8.3:48695253-48695272 None:intergenic 45.0%
ATCACTATTGGCCATACCAG+CGG - chr8.3:48695266-48695285 None:intergenic 45.0%
ATCTTGAGACGACCTGTGAA+GGG - chr8.3:48694410-48694429 None:intergenic 45.0%
CGAATTTCGCTTCTGCTTGT+GGG - chr8.3:48695021-48695040 None:intergenic 45.0%
GAGGAATTGAAGATCCCATG+GGG - chr8.3:48694273-48694292 None:intergenic 45.0%
GCGAAATTCGTGCTGGATAT+TGG + chr8.3:48695031-48695050 MS.gene32261:CDS 45.0%
GGAGGAATTGAAGATCCCAT+GGG - chr8.3:48694274-48694293 None:intergenic 45.0%
TATCTTGAGACGACCTGTGA+AGG - chr8.3:48694411-48694430 None:intergenic 45.0%
TTAGTCGACGGCATCTTCAT+TGG + chr8.3:48694641-48694660 MS.gene32261:CDS 45.0%
! CATTAAAGCCGGTGACTTGT+TGG + chr8.3:48695126-48695145 MS.gene32261:CDS 45.0%
! CCGTTGCCTGAGGATTATTT+TGG + chr8.3:48695076-48695095 MS.gene32261:CDS 45.0%
! CGTCGACTAACTCTGTTACT+TGG - chr8.3:48694631-48694650 None:intergenic 45.0%
! GGTGATAAAGAAGCCCCTTT+TGG - chr8.3:48694310-48694329 None:intergenic 45.0%
! TCATGGGCTGAAATCTCGAA+AGG + chr8.3:48694719-48694738 MS.gene32261:CDS 45.0%
! TGGTAATTGGTGGTTCTCCT+TGG + chr8.3:48695290-48695309 MS.gene32261:CDS 45.0%
!! CCCTTTGTTGTTGCACAAGA+TGG - chr8.3:48694465-48694484 None:intergenic 45.0%
ACCGTCTTCCAACAAGTCAC+CGG - chr8.3:48695137-48695156 None:intergenic 50.0%
AGCAGAAGCGAAATTCGTGC+TGG + chr8.3:48695024-48695043 MS.gene32261:CDS 50.0%
GAAGATCCCATGGGGTTAGA+TGG - chr8.3:48694265-48694284 None:intergenic 50.0%
GCTACGGCATGAACAAAGAG+TGG - chr8.3:48694488-48694507 None:intergenic 50.0%
GGGAGGAATTGAAGATCCCA+TGG - chr8.3:48694275-48694294 None:intergenic 50.0%
GTGTATGCGGGTGTAGAAGA+TGG + chr8.3:48695400-48695419 MS.gene32261:CDS 50.0%
TATGCGGGTGTAGAAGATGG+TGG + chr8.3:48695403-48695422 MS.gene32261:CDS 50.0%
TTTGGTTGGGGGAAACCTGT+GGG + chr8.3:48695334-48695353 MS.gene32261:CDS 50.0%
! GGGGGTGCGAGTAAAAGAAA+TGG + chr8.3:48695367-48695386 MS.gene32261:CDS 50.0%
! GGGTGTAGAAGATGGTGGTA+TGG + chr8.3:48695408-48695427 MS.gene32261:CDS 50.0%
!! GAGTTTTGTCACCGCTGGTA+TGG + chr8.3:48695252-48695271 MS.gene32261:CDS 50.0%
!! GTGACTTGTTGGAAGACGGT+GGG + chr8.3:48695137-48695156 MS.gene32261:CDS 50.0%
!! TTTTGGTTGGGGGAAACCTG+TGG + chr8.3:48695333-48695352 MS.gene32261:CDS 50.0%
AAACCTGTGGGGGTTCGAAG+TGG + chr8.3:48695346-48695365 MS.gene32261:CDS 55.0%
AACCTGTGGGGGTTCGAAGT+GGG + chr8.3:48695347-48695366 MS.gene32261:CDS 55.0%
GGCAACGGAGGAATCAACCT+TGG - chr8.3:48695064-48695083 None:intergenic 55.0%
TTGGTTGGGGGAAACCTGTG+GGG + chr8.3:48695335-48695354 MS.gene32261:CDS 55.0%
! GAAGACGGTGGGCTTGGTAA+GGG + chr8.3:48695148-48695167 MS.gene32261:CDS 55.0%
! GTTGATTCCTCCGTTGCCTG+AGG + chr8.3:48695066-48695085 MS.gene32261:CDS 55.0%
! TGGGCTTGGTAAGGGCTCTT+TGG + chr8.3:48695156-48695175 MS.gene32261:CDS 55.0%
! TTGTTGGAAGACGGTGGGCT+TGG + chr8.3:48695142-48695161 MS.gene32261:CDS 55.0%
!! GCCGGTGACTTGTTGGAAGA+CGG + chr8.3:48695133-48695152 MS.gene32261:CDS 55.0%
!! GGTGACTTGTTGGAAGACGG+TGG + chr8.3:48695136-48695155 MS.gene32261:CDS 55.0%
ACCTGTGGGGGTTCGAAGTG+GGG + chr8.3:48695348-48695367 MS.gene32261:CDS 60.0%
TGGTTGGGGGAAACCTGTGG+GGG + chr8.3:48695336-48695355 MS.gene32261:CDS 60.0%
! GGAAGACGGTGGGCTTGGTA+AGG + chr8.3:48695147-48695166 MS.gene32261:CDS 60.0%
!! GCACCGAGTTTTGTCACCGC+TGG + chr8.3:48695247-48695266 MS.gene32261:CDS 60.0%
CCTGTGGGGGTTCGAAGTGG+GGG + chr8.3:48695349-48695368 MS.gene32261:CDS 65.0%
CCCCCACTTCGAACCCCCAC+AGG - chr8.3:48695352-48695371 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 48694182 48695510 48694182 ID=MS.gene32261
chr8.3 mRNA 48694182 48695510 48694182 ID=MS.gene32261.t1;Parent=MS.gene32261
chr8.3 exon 48694182 48695510 48694182 ID=MS.gene32261.t1.exon1;Parent=MS.gene32261.t1
chr8.3 CDS 48694182 48695510 48694182 ID=cds.MS.gene32261.t1;Parent=MS.gene32261.t1
Gene Sequence

>MS.gene32261

ATGAGTTCCATCAAAGTCCTCTCCACAACTACAATACATGCAGCAAATCATAATCTTTCTGACTCAACTTATCATACAATCCATCTAACCCCATGGGATCTTCAATTCCTCCCATTTGGATTCAACCAAAAGGGGCTTCTTTATCACCACCCTTTCGAATTAGATAGAACAAATCAAATCCAACACCTTAAAGACTCTCTTTCTTCTACCCTTGATATTTTTTGGCCCTTCACAGGTCGTCTCAAGATAACAGAACACGAAGCTAATACTATCTCGTGTTCCATCTTGTGCAACAACAAAGGGCCACTCTTTGTTCATGCCGTAGCAAAAAACATTAGTGTTGCCGACATCGTTAAACCCACTTATCATCCTCCTATTCACCACTCATTTTTTGCTCTAAATGGAGTTAAAAACTATGAAGGAACATCACAACCGTTACTTGCAGTCCAAGTAACAGAGTTAGTCGACGGCATCTTCATTGGCTTCACAGTTAACCACGTGATTGTTGATGGTACGTCGTTTTGGCATTTCATCAATTCATGGGCTGAAATCTCGAAAGGTTGTCTTAAAATATCCAAACTTCCAACATTTGATCGTTGGTTTTCAAAAGGTATTGAATGTCCCATTAGATTTCCTTTCACAATAGAGTCACGAAATGAGGATAATTATGAAGAAGGAAAAATCAACTTTCCAGAGAGAGTATTTCATTTCAAAAAGGAGAATATCGCAAAACTAAAATTCAAAGCAAACTTAGAGGTTGGTACAAAAAATATATCTTCCTTGCAAGCAATTTTCACTCATATTTGGCGCTCTATTATACGTTGCAAAAAACTTGACCCACAAGCAGAAGCGAAATTCGTGCTGGATATTGGAATTAGACCAAGGTTGATTCCTCCGTTGCCTGAGGATTATTTTGGTAATGCTGTGATAGATTGTGTTGTTAGCATTAAAGCCGGTGACTTGTTGGAAGACGGTGGGCTTGGTAAGGGCTCTTTGGAGATTAACAAGATGATTGCCTTTCACACAGATGAGAAGTTGAAAAATCACTATGAAAATTGGTTAATAGCACCGAGTTTTGTCACCGCTGGTATGGCCAATAGTGATTCATTGGTAATTGGTGGTTCTCCTTGGTTTGATGTTTATGGTAATGATTTTGGTTGGGGGAAACCTGTGGGGGTTCGAAGTGGGGGTGCGAGTAAAAGAAATGGAAAGATTTATGTGTATGCGGGTGTAGAAGATGGTGGTATGGACCTTGAAGTATGTCTTCCTTGTGAAATTTTGGAGGCAATTAGAAATGATCCTGAGTTTATGGATGTTGTGTCCTACTAA
Protein sequence

>MS.gene32261.t1

MSSIKVLSTTTIHAANHNLSDSTYHTIHLTPWDLQFLPFGFNQKGLLYHHPFELDRTNQIQHLKDSLSSTLDIFWPFTGRLKITEHEANTISCSILCNNKGPLFVHAVAKNISVADIVKPTYHPPIHHSFFALNGVKNYEGTSQPLLAVQVTELVDGIFIGFTVNHVIVDGTSFWHFINSWAEISKGCLKISKLPTFDRWFSKGIECPIRFPFTIESRNEDNYEEGKINFPERVFHFKKENIAKLKFKANLEVGTKNISSLQAIFTHIWRSIIRCKKLDPQAEAKFVLDIGIRPRLIPPLPEDYFGNAVIDCVVSIKAGDLLEDGGLGKGSLEINKMIAFHTDEKLKNHYENWLIAPSFVTAGMANSDSLVIGGSPWFDVYGNDFGWGKPVGVRSGGASKRNGKIYVYAGVEDGGMDLEVCLPCEILEAIRNDPEFMDVVSY