AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene011801

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011801.t1 MTR_4g094835 71.930 456 117 3 1 454 1 447 0.0 679
MS.gene011801.t1 MTR_4g094838 71.491 456 100 7 4 453 1 432 0.0 630
MS.gene011801.t1 MTR_4g094832 73.404 376 92 3 81 453 60 430 0.0 558
MS.gene011801.t1 MTR_4g094842 60.352 454 165 7 1 449 1 444 0.0 544
MS.gene011801.t1 MTR_0190s0020 58.407 452 171 8 1 449 1 438 0.0 524
MS.gene011801.t1 MTR_8g041770 57.080 452 176 8 1 449 1 437 0.0 513
MS.gene011801.t1 MTR_3g088420 57.650 451 167 10 1 449 1 429 3.58e-177 503
MS.gene011801.t1 MTR_4g094828 56.264 455 176 10 1 445 1 442 9.51e-177 503
MS.gene011801.t1 MTR_4g022720 56.195 452 174 8 1 449 1 431 4.74e-176 500
MS.gene011801.t1 MTR_4g022825 55.799 457 173 10 1 449 1 436 1.18e-174 497
MS.gene011801.t1 MTR_8g079710 57.174 453 168 10 1 444 1 436 4.66e-174 495
MS.gene011801.t1 MTR_4g022800 55.556 459 173 10 1 449 1 438 4.09e-173 493
MS.gene011801.t1 MTR_4g022290 57.143 455 167 12 1 445 1 437 2.98e-172 491
MS.gene011801.t1 MTR_4g022670 54.367 458 179 10 1 449 1 437 2.34e-169 483
MS.gene011801.t1 MTR_3g026310 54.095 464 178 11 1 449 1 444 4.86e-168 480
MS.gene011801.t1 MTR_7g026860 54.048 457 179 10 1 449 1 434 2.07e-164 471
MS.gene011801.t1 MTR_4g022760 49.015 457 161 9 1 449 1 393 5.77e-141 409
MS.gene011801.t1 MTR_6g016815 42.572 451 169 13 1 449 15 377 2.30e-104 315
MS.gene011801.t1 MTR_4g094825 85.143 175 22 2 20 194 80 250 1.50e-94 291
MS.gene011801.t1 MTR_4g094825 83.766 154 15 1 300 453 248 391 1.37e-83 262
MS.gene011801.t1 MTR_3g101900 39.443 431 240 9 28 449 26 444 4.03e-93 289
MS.gene011801.t1 MTR_3g102020 37.931 464 250 11 3 449 5 447 1.31e-91 285
MS.gene011801.t1 MTR_3g101930 38.673 437 234 9 32 449 30 451 8.90e-91 283
MS.gene011801.t1 MTR_3g102010 38.095 462 249 11 4 449 7 447 9.96e-89 278
MS.gene011801.t1 MTR_3g101960 37.093 461 255 10 3 448 5 445 3.96e-87 274
MS.gene011801.t1 MTR_3g101970 37.150 428 236 8 32 449 29 433 1.71e-84 266
MS.gene011801.t1 MTR_5g081960 33.680 481 235 10 30 449 25 482 9.94e-80 255
MS.gene011801.t1 MTR_3g070170 35.637 463 236 10 30 453 31 470 1.91e-79 254
MS.gene011801.t1 MTR_8g040780 57.619 210 84 3 242 448 20 227 8.67e-77 240
MS.gene011801.t1 MTR_8g040760 54.626 227 88 5 1 224 1 215 1.26e-75 236
MS.gene011801.t1 MTR_4g015180 32.444 487 245 11 27 449 17 483 8.96e-69 227
MS.gene011801.t1 MTR_7g026850 54.286 175 70 3 37 209 12 178 6.60e-58 189
MS.gene011801.t1 MTR_6g045553 28.333 480 296 11 2 450 10 472 1.90e-49 175
MS.gene011801.t1 MTR_3g102030 33.007 306 168 4 106 407 48 320 2.81e-48 168
MS.gene011801.t1 MTR_7g026770 49.724 181 64 4 157 335 132 287 1.72e-46 163
MS.gene011801.t1 MTR_6g034485 56.154 130 52 2 202 331 1 125 9.11e-45 152
MS.gene011801.t1 MTR_6g034495 58.594 128 44 2 72 194 37 160 1.05e-43 151
MS.gene011801.t1 MTR_7g026830 68.293 82 26 0 100 181 16 97 3.10e-33 121
MS.gene011801.t1 MTR_4g007540 26.142 394 253 12 64 442 56 426 1.97e-28 117
MS.gene011801.t1 MTR_8g096530 29.477 363 213 13 57 405 52 385 2.53e-27 114
MS.gene011801.t1 MTR_3g098980 26.923 390 245 15 57 435 59 419 5.50e-27 112
MS.gene011801.t1 MTR_3g049150 25.740 439 269 14 37 440 21 437 1.73e-25 108
MS.gene011801.t1 MTR_2g105330 23.869 398 259 10 64 438 56 432 3.40e-25 107
MS.gene011801.t1 MTR_3g026950 24.694 409 255 15 61 438 51 437 6.18e-25 107
MS.gene011801.t1 MTR_2g064495 23.499 383 260 9 64 434 56 417 3.74e-24 104
MS.gene011801.t1 MTR_8g075610 24.433 397 252 15 64 442 47 413 2.48e-23 102
MS.gene011801.t1 MTR_4g097090 24.773 440 259 16 40 434 28 440 2.92e-23 102
MS.gene011801.t1 MTR_4g127650 22.472 445 288 14 22 434 21 440 1.49e-21 97.1
MS.gene011801.t1 MTR_3g071320 25.893 336 205 12 49 362 42 355 5.13e-21 95.5
MS.gene011801.t1 MTR_7g026780 46.296 108 32 3 342 449 26 107 6.10e-21 88.2
MS.gene011801.t1 MTR_1g081660 23.649 444 294 16 32 453 31 451 1.82e-20 94.0
MS.gene011801.t1 MTR_7g027175 25.700 393 259 12 57 440 61 429 3.87e-20 92.8
MS.gene011801.t1 MTR_7g026800 38.272 162 68 6 231 390 29 160 1.01e-19 87.4
MS.gene011801.t1 MTR_6g015850 24.307 469 302 15 2 442 6 449 1.45e-19 91.3
MS.gene011801.t1 MTR_5g026320 25.481 416 244 16 61 448 71 448 1.98e-19 90.9
MS.gene011801.t1 MTR_4g022780 36.970 165 72 6 231 393 30 164 5.05e-19 84.7
MS.gene011801.t1 MTR_5g084870 25.879 398 230 17 33 403 17 376 8.68e-19 88.6
MS.gene011801.t1 MTR_6g015815 24.153 443 284 16 30 442 32 452 1.66e-18 88.2
MS.gene011801.t1 MTR_2g089765 24.583 480 290 20 3 437 15 467 2.56e-18 87.8
MS.gene011801.t1 MTR_3g087640 26.386 451 275 19 30 440 29 462 2.99e-18 87.4
MS.gene011801.t1 MTR_6g015315 25.938 320 200 8 155 442 141 455 3.63e-18 87.0
MS.gene011801.t1 MTR_2g089755 26.585 410 247 20 32 403 37 430 5.72e-18 86.7
MS.gene011801.t1 MTR_3g086020 25.935 401 236 17 62 449 59 411 5.96e-18 85.9
MS.gene011801.t1 MTR_3g049140 27.511 229 140 6 230 440 14 234 1.23e-17 82.8
MS.gene011801.t1 MTR_7g014330 24.631 406 253 15 30 402 24 409 1.28e-17 85.5
MS.gene011801.t1 MTR_1g040195 24.083 436 250 18 33 440 23 405 2.45e-17 84.3
MS.gene011801.t1 MTR_8g012750 25.412 425 239 18 30 402 24 422 6.72e-17 83.2
MS.gene011801.t1 MTR_7g084940 25.205 365 230 12 52 402 57 392 8.70e-17 82.8
MS.gene011801.t1 MTR_7g014250 25.182 413 242 17 30 402 24 409 8.96e-17 82.8
MS.gene011801.t1 MTR_6g015320 26.349 315 189 12 155 440 144 444 1.01e-16 82.4
MS.gene011801.t1 MTR_8g079980 24.000 400 240 11 33 403 17 381 1.97e-16 81.6
MS.gene011801.t1 MTR_2g089670 25.000 412 247 18 29 402 42 429 3.63e-16 80.9
MS.gene011801.t1 MTR_7g015860 23.163 449 271 15 30 434 26 444 4.12e-16 80.9
MS.gene011801.t1 MTR_7g085930 23.786 412 249 14 64 429 59 451 5.80e-16 80.5
MS.gene011801.t1 MTR_5g076700 22.247 445 287 16 33 447 24 439 1.48e-15 79.0
MS.gene011801.t1 MTR_7g114470 23.744 438 270 18 33 437 25 431 3.11e-15 78.2
MS.gene011801.t1 MTR_3g450710 25.707 389 217 16 58 407 59 414 5.23e-15 77.4
MS.gene011801.t1 MTR_7g086000 25.424 413 255 14 62 442 58 449 6.04e-15 77.0
MS.gene011801.t1 MTR_4g013050 21.481 405 243 12 63 444 52 404 9.03e-15 76.3
MS.gene011801.t1 MTR_7g014230 23.821 403 260 13 30 402 26 411 1.09e-14 76.3
MS.gene011801.t1 MTR_4g055170 24.671 304 206 7 64 361 69 355 1.18e-14 76.3
MS.gene011801.t1 MTR_2g089650 24.205 409 272 17 23 403 20 418 1.28e-14 76.3
MS.gene011801.t1 MTR_7g014160 23.044 473 288 17 17 434 4 455 1.77e-14 75.9
MS.gene011801.t1 MTR_7g014610 24.190 463 275 19 23 434 20 457 1.87e-14 75.5
MS.gene011801.t1 MTR_7g014200 25.115 434 237 18 23 402 21 420 4.04e-14 74.7
MS.gene011801.t1 MTR_5g090810 24.866 374 226 14 61 403 63 412 9.75e-14 73.6
MS.gene011801.t1 MTR_1g053315 21.795 390 264 15 64 434 78 445 1.16e-13 73.2
MS.gene011801.t1 MTR_7g114490 23.908 435 257 20 37 440 40 431 1.33e-13 73.2
MS.gene011801.t1 MTR_7g114520 23.858 394 248 19 37 410 41 402 1.89e-13 72.4
MS.gene011801.t1 MTR_5g090880 24.011 379 232 15 57 403 60 414 6.59e-13 70.9
MS.gene011801.t1 MTR_6g053810 23.154 298 191 9 64 345 71 346 1.29e-12 70.1
MS.gene011801.t1 MTR_8g467580 25.866 433 248 20 49 449 41 432 1.48e-12 69.7
MS.gene011801.t1 MTR_1g040315 24.092 303 194 10 33 320 23 304 1.90e-12 69.3
MS.gene011801.t1 MTR_3g028210 23.974 463 298 20 4 434 8 448 2.47e-12 68.9
MS.gene011801.t1 MTR_7g056087 22.738 409 268 15 40 419 30 419 2.99e-12 68.6
MS.gene011801.t1 MTR_7g026880 57.778 45 19 0 405 449 5 49 5.97e-12 60.8
MS.gene011801.t1 MTR_1g094790 24.558 452 253 21 40 449 32 437 1.08e-11 67.0
MS.gene011801.t1 MTR_7g084910 24.104 307 182 9 40 319 36 318 1.45e-11 66.6
MS.gene011801.t1 MTR_7g013850 22.169 415 255 13 37 402 37 432 1.59e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011801.t1 AT5G67150 41.228 456 244 7 2 449 3 442 8.03e-117 350
MS.gene011801.t1 AT3G50300 40.175 458 250 8 1 449 1 443 1.98e-104 318
MS.gene011801.t1 AT3G50270 39.644 449 255 8 6 449 7 444 2.28e-98 302
MS.gene011801.t1 AT3G50280 36.645 453 269 6 1 449 1 439 3.46e-98 302
MS.gene011801.t1 AT5G38130 39.310 435 247 6 5 434 20 442 3.66e-98 302
MS.gene011801.t1 AT5G67160 39.394 429 232 8 25 448 22 427 7.55e-97 298
MS.gene011801.t1 AT5G42830 35.714 434 253 9 28 449 26 445 7.71e-85 268
MS.gene011801.t1 AT2G39980 32.911 474 240 9 30 449 27 476 5.30e-80 256
MS.gene011801.t1 AT5G07860 37.011 435 237 11 32 449 31 445 6.97e-80 255
MS.gene011801.t1 AT5G07850 36.721 433 245 10 32 449 31 449 1.05e-79 254
MS.gene011801.t1 AT5G07870 36.222 450 244 10 32 454 31 464 3.86e-75 243
MS.gene011801.t1 AT5G01210 34.216 453 241 10 36 449 31 465 6.00e-74 240
MS.gene011801.t1 AT3G50280 38.462 312 186 3 141 449 1 309 1.07e-70 226
MS.gene011801.t1 AT5G23940 31.350 437 278 8 28 450 21 449 8.02e-62 209
MS.gene011801.t1 AT3G50290 39.925 268 154 3 138 400 4 269 1.09e-56 189
MS.gene011801.t1 AT2G19070 25.624 441 279 12 35 442 19 443 5.51e-27 113
MS.gene011801.t1 AT3G29590 25.508 443 288 13 24 444 20 442 7.35e-26 109
MS.gene011801.t1 AT3G48720 26.479 355 230 12 57 403 54 385 1.40e-25 108
MS.gene011801.t1 AT5G41040 26.087 391 250 11 57 434 62 426 2.11e-24 105
MS.gene011801.t1 AT5G41040 26.087 391 250 11 57 434 78 442 2.53e-24 105
MS.gene011801.t1 AT5G48930 24.450 409 263 11 49 437 39 421 3.49e-24 104
MS.gene011801.t1 AT1G78990 27.957 372 222 13 51 403 52 396 1.75e-22 100
MS.gene011801.t1 AT3G29635 23.908 435 278 15 30 434 27 438 2.26e-21 96.7
MS.gene011801.t1 AT5G57840 27.952 415 239 18 49 434 39 422 4.45e-21 95.9
MS.gene011801.t1 AT5G39080 23.060 464 290 17 20 442 16 453 3.41e-20 93.2
MS.gene011801.t1 AT5G63560 24.297 391 251 14 57 434 54 412 1.58e-19 90.9
MS.gene011801.t1 AT1G31490 26.173 405 251 15 61 448 59 432 1.63e-19 90.9
MS.gene011801.t1 AT1G24430 23.438 384 243 14 55 426 53 397 2.09e-19 90.5
MS.gene011801.t1 AT5G23970 24.771 436 278 17 28 450 20 418 4.49e-19 89.7
MS.gene011801.t1 AT5G39090 24.886 438 288 19 20 437 16 432 4.06e-18 86.7
MS.gene011801.t1 AT5G39050 24.920 313 200 12 151 435 145 450 9.01e-18 85.9
MS.gene011801.t1 AT1G24420 25.445 393 242 16 74 453 75 429 1.01e-17 85.5
MS.gene011801.t1 AT1G24420 25.445 393 242 16 74 453 75 429 1.01e-17 85.5
MS.gene011801.t1 AT3G29670 24.123 456 298 18 3 434 2 433 1.16e-17 85.5
MS.gene011801.t1 AT3G26040 24.570 407 254 14 60 449 55 425 2.07e-17 84.7
MS.gene011801.t1 AT3G30280 24.766 428 255 20 1 403 1 386 1.33e-16 82.0
MS.gene011801.t1 AT5G47950 23.094 459 299 16 1 449 1 415 1.43e-16 82.0
MS.gene011801.t1 AT2G40230 23.741 417 274 13 1 403 1 387 2.51e-16 81.3
MS.gene011801.t1 AT1G28680 26.275 255 170 6 157 403 143 387 4.43e-16 80.5
MS.gene011801.t1 AT5G61160 26.367 311 197 14 151 440 139 438 6.05e-15 77.0
MS.gene011801.t1 AT1G28680 26.667 210 141 4 157 359 143 346 9.64e-15 76.3
MS.gene011801.t1 AT4G15390 23.944 355 220 15 4 347 8 323 1.92e-14 75.5
MS.gene011801.t1 AT4G15390 23.944 355 220 15 4 347 8 323 1.92e-14 75.5
MS.gene011801.t1 AT5G16410 25.455 330 203 13 95 403 112 419 4.54e-14 74.7
MS.gene011801.t1 AT5G07080 25.402 311 190 12 42 333 44 331 4.64e-14 74.3
MS.gene011801.t1 AT3G47170 23.529 306 199 10 57 342 63 353 8.07e-13 70.5
MS.gene011801.t1 AT1G27620 22.622 389 266 12 64 442 58 421 6.64e-12 67.8
MS.gene011801.t1 AT5G47980 25.362 414 241 20 63 449 60 432 9.59e-12 67.0
MS.gene011801.t1 AT2G25150 24.427 262 182 8 155 406 148 403 1.81e-11 66.2

Find 93 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGATTCTTTCAGCTAAAT+TGG 0.161367 8.1:-26773140 None:intergenic
GTGTCTTTCTTATGAGATTT+TGG 0.228049 8.1:+26773723 MS.gene011801:CDS
CGTTGAAATGAAGGGAGTTT+TGG 0.257934 8.1:-26773025 None:intergenic
TCATTCTTCCCACCCTTTGC+AGG 0.261438 8.1:+26772644 MS.gene011801:CDS
TATGGGAACCTTTCGAGATT+TGG 0.266155 8.1:-26772997 None:intergenic
TACATTCTGCTGAGAAAATA+AGG 0.279160 8.1:+26773479 MS.gene011801:CDS
TTTGGCATTTCGTTAATTCT+TGG 0.304274 8.1:+26772972 MS.gene011801:CDS
GGAAACGCAGTAGTGGTTAA+TGG 0.325663 8.1:+26773379 MS.gene011801:CDS
ATGGGAACCTTTCGAGATTT+GGG 0.326542 8.1:-26772996 None:intergenic
GGTGGAAAAGAAGGCCTCTT+TGG 0.329077 8.1:-26772562 None:intergenic
TGAGATGGTTGATTTGGTTT+TGG 0.333677 8.1:-26772598 None:intergenic
GGGAAATGATGCTGAGTTCT+TGG 0.345700 8.1:+26773753 MS.gene011801:CDS
AAGCACTATTATCTCATGTT+TGG 0.349361 8.1:+26773248 MS.gene011801:CDS
TAAGGAACCATTACGAATCT+TGG 0.357996 8.1:+26773497 MS.gene011801:CDS
TTTGTTTGAGATGGTTGATT+TGG 0.369144 8.1:-26772604 None:intergenic
TTGTAGATGCCAAGTCGTTT+TGG 0.371191 8.1:+26772954 MS.gene011801:CDS
ACCAAGGATGATTCCACCTT+TGG 0.373650 8.1:+26773342 MS.gene011801:CDS
ATTGGAATTTCAATATCGTT+TGG 0.377241 8.1:-26773055 None:intergenic
TTGGAATTTCAATATCGTTT+GGG 0.377876 8.1:-26773054 None:intergenic
AGCTTTCTTCTGAGGAGTCT+TGG 0.397249 8.1:-26773111 None:intergenic
TGTTGTGGATATCACCTTGA+TGG 0.409513 8.1:-26772435 None:intergenic
AAAACCAACTCTAGCAATTC+AGG 0.414621 8.1:+26772880 MS.gene011801:CDS
TTAAGTGACGGCATCTTCAT+TGG 0.417429 8.1:+26772911 MS.gene011801:CDS
CCTTTGGAAGAAGATTATTT+CGG 0.422258 8.1:+26773358 MS.gene011801:CDS
TGTGTGAAAGGGAAGCGTAT+TGG 0.429614 8.1:-26773073 None:intergenic
GTTTGTGGTGTCGTTGTGTT+GGG 0.434146 8.1:-26772681 None:intergenic
CATTCTTCCCACCCTTTGCA+GGG 0.439523 8.1:+26772645 MS.gene011801:CDS
TCCAAAGGTGGAATCATCCT+TGG 0.454320 8.1:-26773343 None:intergenic
TGTTTGTGGTGTCGTTGTGT+TGG 0.462700 8.1:-26772682 None:intergenic
TTTGGTTAGGAATGGGTTGG+TGG 0.465905 8.1:-26772580 None:intergenic
CCGAAATAATCTTCTTCCAA+AGG 0.467292 8.1:-26773358 None:intergenic
AGATTATTATTGTTGTCAAT+TGG 0.472545 8.1:-26772470 None:intergenic
GGGAACCAACGTTGAAATGA+AGG 0.474068 8.1:-26773034 None:intergenic
GACAATTGGAGGTGTGTAGT+TGG 0.479530 8.1:-26772810 None:intergenic
TGGAAAGTGTTTGTTTGAGA+TGG 0.485281 8.1:-26772613 None:intergenic
TTCTCAACCAAGATTCGTAA+TGG 0.489014 8.1:-26773504 None:intergenic
GAGGTTCTAGAGTGTGAAAT+CGG 0.491146 8.1:+26773421 MS.gene011801:CDS
GAATGGGTTGGTGGAAAAGA+AGG 0.503869 8.1:-26772571 None:intergenic
TGTGATTGTATTTGTGTGAA+AGG 0.505410 8.1:-26773085 None:intergenic
CTTCTTCATGGATTCCATCA+AGG 0.511613 8.1:+26772421 None:intergenic
AATGGTGTGAGGATGAAAGC+AGG 0.512088 8.1:+26773397 MS.gene011801:CDS
GTTACCTGAATTGCTAGAGT+TGG 0.515092 8.1:-26772884 None:intergenic
CAGTCACATTAAGTAGAACT+AGG 0.519351 8.1:-26773778 None:intergenic
TGGTTTCAAGTGGAAGGAAT+CGG 0.519621 8.1:-26772538 None:intergenic
GAGGTGTGTAGTTGGATTGA+AGG 0.522441 8.1:-26772802 None:intergenic
TTTGGATGGTTTCAAGTGGA+AGG 0.524015 8.1:-26772544 None:intergenic
GAAAAGAAGGCCTCTTTGGA+TGG 0.529505 8.1:-26772558 None:intergenic
GTGATTGTATTTGTGTGAAA+GGG 0.534325 8.1:-26773084 None:intergenic
CTCCCACTTCGAAACGCCAC+AGG 0.534418 8.1:-26773631 None:intergenic
GGATTCTAAGCTTTCTTCTG+AGG 0.535259 8.1:-26773119 None:intergenic
TTCCGAAGTGGTAGTTCGCT+AGG 0.540203 8.1:+26773538 MS.gene011801:CDS
ACCGAGGTTGTTGCAAATGA+TGG 0.541381 8.1:-26772732 None:intergenic
GATTACAAGACGCCCTGCAA+AGG 0.543778 8.1:-26772657 None:intergenic
AGCCTGTGGCGTTTCGAAGT+GGG 0.545492 8.1:+26773629 MS.gene011801:CDS
AACGAAATGCCAAAACGACT+TGG 0.545630 8.1:-26772963 None:intergenic
AACTCCCTTCATTTCAACGT+TGG 0.545872 8.1:+26773029 MS.gene011801:CDS
GTGGCGTTTCGAAGTGGGAG+TGG 0.549176 8.1:+26773634 MS.gene011801:CDS
ATGGTGTGAGGATGAAAGCA+GGG 0.551554 8.1:+26773398 MS.gene011801:CDS
GTGTTTGCTGGTGCTGAAGA+AGG 0.553326 8.1:+26773682 MS.gene011801:CDS
ATTACAAGACGCCCTGCAAA+GGG 0.555357 8.1:-26772656 None:intergenic
TTCGCTAGGTGGTAATATGT+TGG 0.564328 8.1:+26773552 MS.gene011801:CDS
AAATAATCTTCTTCCAAAGG+TGG 0.566011 8.1:-26773355 None:intergenic
TGGTGCTTGAATTGTTGTTG+TGG 0.571268 8.1:-26772450 None:intergenic
GGAACCAACGTTGAAATGAA+GGG 0.576282 8.1:-26773033 None:intergenic
TCTTGGTCCCAAATCTCGAA+AGG 0.578370 8.1:+26772989 MS.gene011801:CDS
AACTCTAGCAATTCAGGTAA+CGG 0.581378 8.1:+26772886 MS.gene011801:CDS
GAAAATTGATCTAACACCAT+GGG 0.583475 8.1:+26772511 MS.gene011801:CDS
CACCTAGCGAACTACCACTT+CGG 0.583535 8.1:-26773540 None:intergenic
CCTCTTTGGATGGTTTCAAG+TGG 0.585328 8.1:-26772548 None:intergenic
TCCATCATTTGCAACAACCT+CGG 0.586818 8.1:+26772731 MS.gene011801:CDS
ACATAGAAAACATCTTCTTG+TGG 0.596961 8.1:-26773301 None:intergenic
GGGTGGGAAGAATGAGAGTG+TGG 0.597075 8.1:-26772636 None:intergenic
GGAAGGAATCGGAGATCCCA+TGG 0.597088 8.1:-26772527 None:intergenic
AAGCCTGTGGCGTTTCGAAG+TGG 0.599152 8.1:+26773628 MS.gene011801:CDS
ACAAGACGCCCTGCAAAGGG+TGG 0.600540 8.1:-26772653 None:intergenic
ACACCAACGTTGTTCCGAAG+TGG 0.610004 8.1:+26773526 MS.gene011801:CDS
TGGGAAGAATGAGAGTGTGG+TGG 0.610635 8.1:-26772633 None:intergenic
TTATTTCGGAAACGCAGTAG+TGG 0.625782 8.1:+26773372 MS.gene011801:CDS
AGAAAATTGATCTAACACCA+TGG 0.626932 8.1:+26772510 MS.gene011801:CDS
CCACTTGAAACCATCCAAAG+AGG 0.628828 8.1:+26772548 MS.gene011801:CDS
CTACCACTTCGGAACAACGT+TGG 0.629293 8.1:-26773529 None:intergenic
CAAGACGCCCTGCAAAGGGT+GGG 0.638377 8.1:-26772652 None:intergenic
AAGAATCTTCCATTTCACAA+AGG 0.647468 8.1:+26773153 MS.gene011801:CDS
TGGTGTGAGGATGAAAGCAG+GGG 0.647651 8.1:+26773399 MS.gene011801:CDS
CGAAGTGGTAGTTCGCTAGG+TGG 0.653912 8.1:+26773541 MS.gene011801:CDS
CAGGTAACGGAGTTAAGTGA+CGG 0.655221 8.1:+26772899 MS.gene011801:CDS
AGCACAAAACACAACTGTCG+CGG 0.659280 8.1:+26772775 MS.gene011801:CDS
TGTGAGGATGAAAGCAGGGG+AGG 0.662636 8.1:+26773402 MS.gene011801:CDS
CAGTAGTGGTTAATGGTGTG+AGG 0.690545 8.1:+26773386 MS.gene011801:CDS
TTTCACAAAGGAGAAAATCG+CGG 0.713213 8.1:+26773165 MS.gene011801:CDS
ACTTGGCATCTACAACAGCG+TGG 0.776414 8.1:-26772946 None:intergenic
TGATGATAGATGTTAGACCA+AGG 0.780037 8.1:+26773326 MS.gene011801:CDS
CGTGGACGAAGAGTGCACCG+AGG 0.794757 8.1:-26772748 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTAGTTTTATCTGTTT+CGG - chr8.1:26773218-26773237 None:intergenic 15.0%
!! AGATTATTATTGTTGTCAAT+TGG - chr8.1:26772473-26772492 None:intergenic 20.0%
! ATTGGAATTTCAATATCGTT+TGG - chr8.1:26773058-26773077 None:intergenic 25.0%
! TTGGAATTTCAATATCGTTT+GGG - chr8.1:26773057-26773076 None:intergenic 25.0%
!!! CACTCATTTTTTACACTTAA+CGG + chr8.1:26772833-26772852 MS.gene011801:CDS 25.0%
AAATAATCTTCTTCCAAAGG+TGG - chr8.1:26773358-26773377 None:intergenic 30.0%
AAGAATCTTCCATTTCACAA+AGG + chr8.1:26773153-26773172 MS.gene011801:CDS 30.0%
AAGCACTATTATCTCATGTT+TGG + chr8.1:26773248-26773267 MS.gene011801:CDS 30.0%
ACATAGAAAACATCTTCTTG+TGG - chr8.1:26773304-26773323 None:intergenic 30.0%
AGAAAATTGATCTAACACCA+TGG + chr8.1:26772510-26772529 MS.gene011801:CDS 30.0%
CGTTAAGTGTAAAAAATGAG+TGG - chr8.1:26772835-26772854 None:intergenic 30.0%
GAAAATTGATCTAACACCAT+GGG + chr8.1:26772511-26772530 MS.gene011801:CDS 30.0%
GAAGATTCTTTCAGCTAAAT+TGG - chr8.1:26773143-26773162 None:intergenic 30.0%
GGGAGTGGTAATAAAAATAA+TGG + chr8.1:26773649-26773668 MS.gene011801:CDS 30.0%
GTAAAAAATGAGTGGACAAT+TGG - chr8.1:26772827-26772846 None:intergenic 30.0%
GTGATTGTATTTGTGTGAAA+GGG - chr8.1:26773087-26773106 None:intergenic 30.0%
TACATTCTGCTGAGAAAATA+AGG + chr8.1:26773479-26773498 MS.gene011801:CDS 30.0%
TGTGATTGTATTTGTGTGAA+AGG - chr8.1:26773088-26773107 None:intergenic 30.0%
TTGCCAAAAACATCAAATCT+CGG - chr8.1:26773592-26773611 None:intergenic 30.0%
TTTGGCATTTCGTTAATTCT+TGG + chr8.1:26772972-26772991 MS.gene011801:CDS 30.0%
! CCTTTGGAAGAAGATTATTT+CGG + chr8.1:26773358-26773377 MS.gene011801:CDS 30.0%
! GTGTCTTTCTTATGAGATTT+TGG + chr8.1:26773723-26773742 MS.gene011801:CDS 30.0%
! TCTTTCTTATGAGATTTTGG+AGG + chr8.1:26773726-26773745 MS.gene011801:CDS 30.0%
! TTTGTTTGAGATGGTTGATT+TGG - chr8.1:26772607-26772626 None:intergenic 30.0%
!! AAGGGAGTTTTGGTATTTTA+TGG - chr8.1:26773018-26773037 None:intergenic 30.0%
!! AGGGAGTTTTGGTATTTTAT+GGG - chr8.1:26773017-26773036 None:intergenic 30.0%
!! TTTTTGGCAATGATTTTGGT+TGG + chr8.1:26773602-26773621 MS.gene011801:CDS 30.0%
!!! ATTTGGTTTTGGTTAGGAAT+GGG - chr8.1:26772590-26772609 None:intergenic 30.0%
!!! GATGTTTTTGGCAATGATTT+TGG + chr8.1:26773598-26773617 MS.gene011801:CDS 30.0%
!!! TTTTGGCAATGATTTTGGTT+GGG + chr8.1:26773603-26773622 MS.gene011801:CDS 30.0%
AAAAATGAGTGGACAATTGG+AGG - chr8.1:26772824-26772843 None:intergenic 35.0%
AAAACCAACTCTAGCAATTC+AGG + chr8.1:26772880-26772899 MS.gene011801:CDS 35.0%
AACTCTAGCAATTCAGGTAA+CGG + chr8.1:26772886-26772905 MS.gene011801:CDS 35.0%
CCGAAATAATCTTCTTCCAA+AGG - chr8.1:26773361-26773380 None:intergenic 35.0%
GGAAAAATTAGTGTGTTTGC+TGG + chr8.1:26773670-26773689 MS.gene011801:CDS 35.0%
TAAGGAACCATTACGAATCT+TGG + chr8.1:26773497-26773516 MS.gene011801:CDS 35.0%
TGATGATAGATGTTAGACCA+AGG + chr8.1:26773326-26773345 MS.gene011801:CDS 35.0%
TTCTCAACCAAGATTCGTAA+TGG - chr8.1:26773507-26773526 None:intergenic 35.0%
TTTCACAAAGGAGAAAATCG+CGG + chr8.1:26773165-26773184 MS.gene011801:CDS 35.0%
! CGATTTTCTCCTTTGTGAAA+TGG - chr8.1:26773165-26773184 None:intergenic 35.0%
! TCTCCGAGATTTGATGTTTT+TGG + chr8.1:26773586-26773605 MS.gene011801:CDS 35.0%
!! GTTGTTGTTGTTGTTGTTTG+TGG - chr8.1:26772699-26772718 None:intergenic 35.0%
!! TGGAAAGTGTTTGTTTGAGA+TGG - chr8.1:26772616-26772635 None:intergenic 35.0%
!! TTATGAGATTTTGGAGGCAT+TGG + chr8.1:26773732-26773751 MS.gene011801:CDS 35.0%
!!! GATTTGGTTTTGGTTAGGAA+TGG - chr8.1:26772591-26772610 None:intergenic 35.0%
!!! TATGAGATTTTGGAGGCATT+GGG + chr8.1:26773733-26773752 MS.gene011801:CDS 35.0%
!!! TGAGATGGTTGATTTGGTTT+TGG - chr8.1:26772601-26772620 None:intergenic 35.0%
!!! TGGTTGATTTGGTTTTGGTT+AGG - chr8.1:26772596-26772615 None:intergenic 35.0%
!!! TTTGGCAATGATTTTGGTTG+GGG + chr8.1:26773604-26773623 MS.gene011801:CDS 35.0%
AACGAAATGCCAAAACGACT+TGG - chr8.1:26772966-26772985 None:intergenic 40.0%
AACTCCCTTCATTTCAACGT+TGG + chr8.1:26773029-26773048 MS.gene011801:CDS 40.0%
ATGGGAACCTTTCGAGATTT+GGG - chr8.1:26772999-26773018 None:intergenic 40.0%
GGAACCAACGTTGAAATGAA+GGG - chr8.1:26773036-26773055 None:intergenic 40.0%
GGATTCTAAGCTTTCTTCTG+AGG - chr8.1:26773122-26773141 None:intergenic 40.0%
GTTACCTGAATTGCTAGAGT+TGG - chr8.1:26772887-26772906 None:intergenic 40.0%
TATGGGAACCTTTCGAGATT+TGG - chr8.1:26773000-26773019 None:intergenic 40.0%
TCCATCATTTGCAACAACCT+CGG + chr8.1:26772731-26772750 MS.gene011801:CDS 40.0%
TGTTGTGGATATCACCTTGA+TGG - chr8.1:26772438-26772457 None:intergenic 40.0%
TTAAGTGACGGCATCTTCAT+TGG + chr8.1:26772911-26772930 MS.gene011801:CDS 40.0%
TTATTTCGGAAACGCAGTAG+TGG + chr8.1:26773372-26773391 MS.gene011801:CDS 40.0%
TTCGCTAGGTGGTAATATGT+TGG + chr8.1:26773552-26773571 MS.gene011801:CDS 40.0%
! CGTTGAAATGAAGGGAGTTT+TGG - chr8.1:26773028-26773047 None:intergenic 40.0%
! TGGTGCTTGAATTGTTGTTG+TGG - chr8.1:26772453-26772472 None:intergenic 40.0%
! TGGTTTCAAGTGGAAGGAAT+CGG - chr8.1:26772541-26772560 None:intergenic 40.0%
! TTGTAGATGCCAAGTCGTTT+TGG + chr8.1:26772954-26772973 MS.gene011801:CDS 40.0%
! TTTGGATGGTTTCAAGTGGA+AGG - chr8.1:26772547-26772566 None:intergenic 40.0%
!! GAGGTTCTAGAGTGTGAAAT+CGG + chr8.1:26773421-26773440 MS.gene011801:CDS 40.0%
AATGGTGTGAGGATGAAAGC+AGG + chr8.1:26773397-26773416 MS.gene011801:CDS 45.0%
ACCAAGGATGATTCCACCTT+TGG + chr8.1:26773342-26773361 MS.gene011801:CDS 45.0%
ACCGAGGTTGTTGCAAATGA+TGG - chr8.1:26772735-26772754 None:intergenic 45.0%
AGCACAAAACACAACTGTCG+CGG + chr8.1:26772775-26772794 MS.gene011801:CDS 45.0%
ATGGTGTGAGGATGAAAGCA+GGG + chr8.1:26773398-26773417 MS.gene011801:CDS 45.0%
ATTACAAGACGCCCTGCAAA+GGG - chr8.1:26772659-26772678 None:intergenic 45.0%
CAGTAGTGGTTAATGGTGTG+AGG + chr8.1:26773386-26773405 MS.gene011801:CDS 45.0%
CCACTTGAAACCATCCAAAG+AGG + chr8.1:26772548-26772567 MS.gene011801:CDS 45.0%
CCTCTTTGGATGGTTTCAAG+TGG - chr8.1:26772551-26772570 None:intergenic 45.0%
GAAAAGAAGGCCTCTTTGGA+TGG - chr8.1:26772561-26772580 None:intergenic 45.0%
GAGGTGTGTAGTTGGATTGA+AGG - chr8.1:26772805-26772824 None:intergenic 45.0%
GGAAACGCAGTAGTGGTTAA+TGG + chr8.1:26773379-26773398 MS.gene011801:CDS 45.0%
GGGAAATGATGCTGAGTTCT+TGG + chr8.1:26773753-26773772 MS.gene011801:CDS 45.0%
GGGAACCAACGTTGAAATGA+AGG - chr8.1:26773037-26773056 None:intergenic 45.0%
TCCAAAGGTGGAATCATCCT+TGG - chr8.1:26773346-26773365 None:intergenic 45.0%
TCTTGGTCCCAAATCTCGAA+AGG + chr8.1:26772989-26773008 MS.gene011801:CDS 45.0%
! AGCTTTCTTCTGAGGAGTCT+TGG - chr8.1:26773114-26773133 None:intergenic 45.0%
! GACAATTGGAGGTGTGTAGT+TGG - chr8.1:26772813-26772832 None:intergenic 45.0%
! GACAGTTGTGTTTTGTGCTG+TGG - chr8.1:26772774-26772793 None:intergenic 45.0%
! TGTGTGAAAGGGAAGCGTAT+TGG - chr8.1:26773076-26773095 None:intergenic 45.0%
!! CAGGTAACGGAGTTAAGTGA+CGG + chr8.1:26772899-26772918 MS.gene011801:CDS 45.0%
!! GAATGGGTTGGTGGAAAAGA+AGG - chr8.1:26772574-26772593 None:intergenic 45.0%
!! GTTTGTGGTGTCGTTGTGTT+GGG - chr8.1:26772684-26772703 None:intergenic 45.0%
!! TGTTTGTGGTGTCGTTGTGT+TGG - chr8.1:26772685-26772704 None:intergenic 45.0%
!! TTTGGTTAGGAATGGGTTGG+TGG - chr8.1:26772583-26772602 None:intergenic 45.0%
!!! GGTTTTGGTTAGGAATGGGT+TGG - chr8.1:26772586-26772605 None:intergenic 45.0%
ACACCAACGTTGTTCCGAAG+TGG + chr8.1:26773526-26773545 MS.gene011801:CDS 50.0%
ACTTGGCATCTACAACAGCG+TGG - chr8.1:26772949-26772968 None:intergenic 50.0%
CACCTAGCGAACTACCACTT+CGG - chr8.1:26773543-26773562 None:intergenic 50.0%
CATTCTTCCCACCCTTTGCA+GGG + chr8.1:26772645-26772664 MS.gene011801:CDS 50.0%
CTACCACTTCGGAACAACGT+TGG - chr8.1:26773532-26773551 None:intergenic 50.0%
GATTACAAGACGCCCTGCAA+AGG - chr8.1:26772660-26772679 None:intergenic 50.0%
GGTGGAAAAGAAGGCCTCTT+TGG - chr8.1:26772565-26772584 None:intergenic 50.0%
TCATTCTTCCCACCCTTTGC+AGG + chr8.1:26772644-26772663 MS.gene011801:CDS 50.0%
TGGTGTGAGGATGAAAGCAG+GGG + chr8.1:26773399-26773418 MS.gene011801:CDS 50.0%
TTCCGAAGTGGTAGTTCGCT+AGG + chr8.1:26773538-26773557 MS.gene011801:CDS 50.0%
! GTGTTTGCTGGTGCTGAAGA+AGG + chr8.1:26773682-26773701 MS.gene011801:CDS 50.0%
! TGGGAAGAATGAGAGTGTGG+TGG - chr8.1:26772636-26772655 None:intergenic 50.0%
! TTGTGTTTTGTGCTGTGGCG+TGG - chr8.1:26772769-26772788 None:intergenic 50.0%
!! TTTTGGTTGGGGAAAGCCTG+TGG + chr8.1:26773615-26773634 MS.gene011801:CDS 50.0%
AAGCCTGTGGCGTTTCGAAG+TGG + chr8.1:26773628-26773647 MS.gene011801:CDS 55.0%
AGCCTGTGGCGTTTCGAAGT+GGG + chr8.1:26773629-26773648 MS.gene011801:CDS 55.0%
CGAAGTGGTAGTTCGCTAGG+TGG + chr8.1:26773541-26773560 MS.gene011801:CDS 55.0%
GGAAGGAATCGGAGATCCCA+TGG - chr8.1:26772530-26772549 None:intergenic 55.0%
GGGTGGGAAGAATGAGAGTG+TGG - chr8.1:26772639-26772658 None:intergenic 55.0%
TGTGAGGATGAAAGCAGGGG+AGG + chr8.1:26773402-26773421 MS.gene011801:CDS 55.0%
ACAAGACGCCCTGCAAAGGG+TGG - chr8.1:26772656-26772675 None:intergenic 60.0%
CAAGACGCCCTGCAAAGGGT+GGG - chr8.1:26772655-26772674 None:intergenic 60.0%
CTCCCACTTCGAAACGCCAC+AGG - chr8.1:26773634-26773653 None:intergenic 60.0%
GTGGCGTTTCGAAGTGGGAG+TGG + chr8.1:26773634-26773653 MS.gene011801:CDS 60.0%
! CGTGGACGAAGAGTGCACCG+AGG - chr8.1:26772751-26772770 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 26772428 26773792 26772428 ID=MS.gene011801
chr8.1 mRNA 26772428 26773792 26772428 ID=MS.gene011801.t1;Parent=MS.gene011801
chr8.1 exon 26772428 26773792 26772428 ID=MS.gene011801.t1.exon1;Parent=MS.gene011801.t1
chr8.1 CDS 26772428 26773792 26772428 ID=cds.MS.gene011801.t1;Parent=MS.gene011801.t1
Gene Sequence

>MS.gene011801

ATGGATTCCATCAAGGTGATATCCACAACAACAATTCAAGCACCAATTGACAACAATAATAATCTCAATTCAACTGATTATCAGAAAATTGATCTAACACCATGGGATCTCCGATTCCTTCCACTTGAAACCATCCAAAGAGGCCTTCTTTTCCACCAACCCATTCCTAACCAAAACCAAATCAACCATCTCAAACAAACACTTTCCACCACACTCTCATTCTTCCCACCCTTTGCAGGGCGTCTTGTAATCACCCAACACAACGACACCACAAACAACAACAACAACAACAACACATCATGTTCCATCATTTGCAACAACCTCGGTGCACTCTTCGTCCACGCCACAGCACAAAACACAACTGTCGCGGACATCCTTCAATCCAACTACACACCTCCAATTGTCCACTCATTTTTTACACTTAACGGTGTTAAAAACTACGAAGCTACGTCAAAACCAACTCTAGCAATTCAGGTAACGGAGTTAAGTGACGGCATCTTCATTGGCTTCACATGCAACCACGCTGTTGTAGATGCCAAGTCGTTTTGGCATTTCGTTAATTCTTGGTCCCAAATCTCGAAAGGTTCCCATAAAATACCAAAACTCCCTTCATTTCAACGTTGGTTCCCAAACGATATTGAAATTCCAATACGCTTCCCTTTCACACAAATACAATCACAAAACCAAGACTCCTCAGAAGAAAGCTTAGAATCCAATTTAGCTGAAAGAATCTTCCATTTCACAAAGGAGAAAATCGCGGAACTGAAATCAAAAGCAAACGCAGAAGCCGAAACAGATAAAACTAAAATATCTTCGCTTCAAGCACTATTATCTCATGTTTGGCGTTCTGTTGTTCGTTGCAAAAAACACGATCCACAAGAAGATGTTTTCTATGTGTTGATGATAGATGTTAGACCAAGGATGATTCCACCTTTGGAAGAAGATTATTTCGGAAACGCAGTAGTGGTTAATGGTGTGAGGATGAAAGCAGGGGAGGTTCTAGAGTGTGAAATCGGAAAAGTTGCTTTAGAGATAAACAAGATGATTTCGTTACATTCTGCTGAGAAAATAAGGAACCATTACGAATCTTGGTTGAGAACACCAACGTTGTTCCGAAGTGGTAGTTCGCTAGGTGGTAATATGTTGGCAATGAGCAGTTCTCCGAGATTTGATGTTTTTGGCAATGATTTTGGTTGGGGAAAGCCTGTGGCGTTTCGAAGTGGGAGTGGTAATAAAAATAATGGAAAAATTAGTGTGTTTGCTGGTGCTGAAGAAGGTAGTATAGATGTTGAAATGTGTCTTTCTTATGAGATTTTGGAGGCATTGGGAAATGATGCTGAGTTCTTGGTTCCTAGTTCTACTTAA
Protein sequence

>MS.gene011801.t1

MDSIKVISTTTIQAPIDNNNNLNSTDYQKIDLTPWDLRFLPLETIQRGLLFHQPIPNQNQINHLKQTLSTTLSFFPPFAGRLVITQHNDTTNNNNNNNTSCSIICNNLGALFVHATAQNTTVADILQSNYTPPIVHSFFTLNGVKNYEATSKPTLAIQVTELSDGIFIGFTCNHAVVDAKSFWHFVNSWSQISKGSHKIPKLPSFQRWFPNDIEIPIRFPFTQIQSQNQDSSEESLESNLAERIFHFTKEKIAELKSKANAEAETDKTKISSLQALLSHVWRSVVRCKKHDPQEDVFYVLMIDVRPRMIPPLEEDYFGNAVVVNGVRMKAGEVLECEIGKVALEINKMISLHSAEKIRNHYESWLRTPTLFRSGSSLGGNMLAMSSSPRFDVFGNDFGWGKPVAFRSGSGNKNNGKISVFAGAEEGSIDVEMCLSYEILEALGNDAEFLVPSST