AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50382

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50382.t1 MTR_4g022670 85.034 441 66 0 1 441 1 441 0.0 789
MS.gene50382.t1 MTR_4g022800 83.710 442 71 1 1 441 1 442 0.0 781
MS.gene50382.t1 MTR_4g022720 83.220 441 68 1 1 441 1 435 0.0 766
MS.gene50382.t1 MTR_7g026860 80.726 441 82 1 1 441 1 438 0.0 753
MS.gene50382.t1 MTR_4g022760 80.726 441 41 1 1 441 1 397 0.0 724
MS.gene50382.t1 MTR_4g022825 73.243 441 117 1 1 441 1 440 0.0 667
MS.gene50382.t1 MTR_3g088420 66.667 441 139 2 1 441 1 433 0.0 592
MS.gene50382.t1 MTR_3g026310 65.848 448 146 3 1 441 1 448 0.0 588
MS.gene50382.t1 MTR_0190s0020 58.407 452 167 7 1 441 1 442 0.0 515
MS.gene50382.t1 MTR_8g041770 57.616 453 168 7 1 441 1 441 4.09e-175 498
MS.gene50382.t1 MTR_4g094842 55.991 459 169 10 1 439 1 446 2.32e-165 473
MS.gene50382.t1 MTR_4g094835 53.187 455 182 8 1 437 1 442 2.59e-159 457
MS.gene50382.t1 MTR_4g094838 50.433 462 177 9 4 442 1 433 3.13e-146 424
MS.gene50382.t1 MTR_4g022290 50.759 461 187 13 1 439 1 443 1.18e-145 422
MS.gene50382.t1 MTR_8g079710 50.773 453 185 11 1 432 1 436 6.03e-143 416
MS.gene50382.t1 MTR_6g016815 51.701 441 139 7 1 441 15 381 4.49e-141 409
MS.gene50382.t1 MTR_4g094832 55.670 388 145 6 69 442 57 431 8.05e-140 407
MS.gene50382.t1 MTR_4g094828 49.675 461 201 12 1 441 1 450 2.01e-139 407
MS.gene50382.t1 MTR_3g101930 39.414 444 239 10 16 440 22 454 8.11e-92 285
MS.gene50382.t1 MTR_7g026770 70.202 198 31 1 141 338 132 301 8.68e-92 280
MS.gene50382.t1 MTR_3g101900 39.359 437 240 11 18 441 24 448 1.05e-90 282
MS.gene50382.t1 MTR_7g026850 72.832 173 46 1 23 195 7 178 6.84e-89 268
MS.gene50382.t1 MTR_3g102020 38.462 442 245 10 16 441 21 451 5.95e-88 275
MS.gene50382.t1 MTR_8g040760 62.441 213 73 3 1 207 1 212 1.37e-85 261
MS.gene50382.t1 MTR_3g101960 38.215 437 242 11 16 436 21 445 2.24e-84 266
MS.gene50382.t1 MTR_3g101970 37.130 439 239 10 16 440 21 436 4.65e-83 262
MS.gene50382.t1 MTR_3g102010 37.557 442 248 11 16 441 22 451 6.30e-83 262
MS.gene50382.t1 MTR_8g040780 58.796 216 85 2 225 436 12 227 8.21e-79 245
MS.gene50382.t1 MTR_3g070170 31.875 480 268 13 2 441 10 470 1.24e-59 202
MS.gene50382.t1 MTR_4g015180 32.305 486 252 14 16 441 19 487 4.78e-58 198
MS.gene50382.t1 MTR_5g081960 30.000 490 259 14 16 442 19 487 1.99e-56 194
MS.gene50382.t1 MTR_4g094825 50.794 189 84 5 17 199 84 269 5.91e-51 177
MS.gene50382.t1 MTR_4g094825 51.572 159 64 2 286 442 245 392 8.46e-45 160
MS.gene50382.t1 MTR_3g102030 34.839 310 163 5 90 397 48 320 2.38e-49 171
MS.gene50382.t1 MTR_6g045553 29.707 478 272 13 4 437 14 471 1.23e-48 173
MS.gene50382.t1 MTR_7g026800 51.163 172 53 3 211 380 18 160 2.42e-46 159
MS.gene50382.t1 MTR_4g022780 50.595 168 53 2 211 377 19 157 1.03e-45 156
MS.gene50382.t1 MTR_6g034495 58.955 134 47 2 54 179 28 161 4.48e-45 154
MS.gene50382.t1 MTR_6g034485 61.364 132 43 2 189 320 2 125 1.54e-44 152
MS.gene50382.t1 MTR_7g026830 77.273 88 20 0 79 166 11 98 1.12e-42 146
MS.gene50382.t1 MTR_7g026780 65.455 110 14 2 332 441 26 111 9.96e-39 136
MS.gene50382.t1 MTR_8g075610 25.858 379 262 10 55 428 47 411 8.30e-30 120
MS.gene50382.t1 MTR_4g007540 25.508 443 270 16 16 428 12 424 2.03e-28 116
MS.gene50382.t1 MTR_2g064495 28.272 382 242 14 55 423 56 418 2.75e-27 113
MS.gene50382.t1 MTR_2g105330 28.272 382 242 14 55 423 56 418 3.57e-27 113
MS.gene50382.t1 MTR_7g026870 88.889 54 6 0 298 351 98 151 8.30e-27 105
MS.gene50382.t1 MTR_7g026880 83.019 53 9 0 390 442 2 54 7.05e-26 99.8
MS.gene50382.t1 MTR_8g096530 29.545 352 210 16 53 392 57 382 4.91e-23 101
MS.gene50382.t1 MTR_3g026950 25.485 412 245 14 52 426 51 437 7.92e-23 100
MS.gene50382.t1 MTR_4g013050 27.548 363 197 14 50 393 48 363 1.14e-21 97.1
MS.gene50382.t1 MTR_2g089755 24.208 442 293 13 24 432 37 469 2.28e-21 96.7
MS.gene50382.t1 MTR_2g089650 24.499 449 291 17 4 418 9 443 4.23e-21 95.9
MS.gene50382.t1 MTR_3g098980 27.095 358 208 17 55 393 66 389 1.74e-20 93.6
MS.gene50382.t1 MTR_3g049150 25.871 402 251 17 52 426 52 433 8.23e-20 91.7
MS.gene50382.t1 MTR_3g028210 26.087 460 282 16 4 423 8 449 2.78e-19 90.5
MS.gene50382.t1 MTR_6g015830 24.944 449 263 15 22 432 42 454 1.34e-18 88.2
MS.gene50382.t1 MTR_3g086020 27.352 457 269 20 4 442 5 416 4.32e-18 86.3
MS.gene50382.t1 MTR_7g014250 25.783 415 249 15 19 396 21 413 5.08e-18 86.3
MS.gene50382.t1 MTR_7g014330 25.962 416 249 16 18 396 20 413 5.47e-18 86.3
MS.gene50382.t1 MTR_7g014230 27.972 429 224 20 18 396 22 415 5.49e-18 86.3
MS.gene50382.t1 MTR_8g012750 24.709 429 276 11 3 392 2 422 7.36e-18 85.9
MS.gene50382.t1 MTR_2g089670 26.632 383 240 16 38 392 60 429 8.24e-18 85.9
MS.gene50382.t1 MTR_3g087640 25.159 473 302 15 1 432 5 466 9.07e-18 85.9
MS.gene50382.t1 MTR_2g089765 25.170 441 258 17 3 392 15 434 2.95e-17 84.3
MS.gene50382.t1 MTR_5g090830 25.195 385 233 13 43 393 49 412 3.45e-17 84.0
MS.gene50382.t1 MTR_7g114490 26.014 419 259 19 44 439 52 442 7.27e-17 83.2
MS.gene50382.t1 MTR_1g040175 29.242 277 172 12 55 324 64 323 1.52e-16 82.0
MS.gene50382.t1 MTR_7g084940 29.474 285 169 12 53 324 67 332 1.89e-16 81.6
MS.gene50382.t1 MTR_4g097090 25.275 364 237 11 55 398 65 413 3.83e-16 80.9
MS.gene50382.t1 MTR_7g027175 25.145 346 231 13 55 393 68 392 4.55e-16 80.5
MS.gene50382.t1 MTR_6g015850 24.541 436 278 15 22 432 42 451 5.08e-16 80.5
MS.gene50382.t1 MTR_1g040215 26.343 391 240 18 25 393 23 387 9.85e-16 79.3
MS.gene50382.t1 MTR_2g089735 25.285 439 281 18 28 432 30 455 1.74e-15 78.6
MS.gene50382.t1 MTR_7g114470 23.165 436 277 17 25 428 25 434 2.55e-15 78.2
MS.gene50382.t1 MTR_7g114520 23.785 391 253 14 25 393 25 392 2.94e-15 78.2
MS.gene50382.t1 MTR_3g450710 27.390 387 220 17 43 393 50 411 4.36e-15 77.4
MS.gene50382.t1 MTR_6g015815 23.928 443 275 18 22 432 42 454 5.15e-15 77.4
MS.gene50382.t1 MTR_7g114550 24.717 441 272 18 25 433 24 436 9.36e-15 76.3
MS.gene50382.t1 MTR_4g127650 24.297 391 257 12 55 422 66 440 1.25e-14 75.9
MS.gene50382.t1 MTR_3g071320 26.174 298 201 8 48 334 54 343 3.61e-14 74.3
MS.gene50382.t1 MTR_5g076700 23.356 441 279 17 25 433 24 437 6.69e-14 73.9
MS.gene50382.t1 MTR_7g014360 28.929 280 152 10 138 396 90 343 6.91e-14 73.2
MS.gene50382.t1 MTR_8g079970 24.648 426 265 17 4 405 3 396 7.15e-14 73.6
MS.gene50382.t1 MTR_7g114560 27.451 255 157 11 41 285 48 284 9.20e-14 73.2
MS.gene50382.t1 MTR_3g112180 23.907 389 270 12 49 428 55 426 1.25e-13 72.8
MS.gene50382.t1 MTR_1g040315 27.707 314 189 13 25 318 23 318 6.55e-13 70.5
MS.gene50382.t1 MTR_4g055170 26.557 305 198 10 55 351 69 355 2.11e-12 69.3
MS.gene50382.t1 MTR_7g084910 26.950 282 168 12 53 317 68 328 5.30e-12 67.8
MS.gene50382.t1 MTR_7g056087 23.797 395 259 17 54 428 57 429 8.06e-12 67.4
MS.gene50382.t1 MTR_3g049140 27.619 210 128 8 235 426 27 230 2.10e-11 64.3
MS.gene50382.t1 MTR_7g086000 25.183 409 241 17 58 426 63 446 2.47e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50382.t1 AT5G67150 39.955 448 242 12 6 437 6 442 1.70e-96 297
MS.gene50382.t1 AT3G50300 40.826 436 232 13 1 422 1 424 5.72e-92 285
MS.gene50382.t1 AT5G67160 39.414 444 238 11 6 441 12 432 1.95e-90 281
MS.gene50382.t1 AT3G50280 37.556 450 259 10 1 438 1 440 5.43e-89 278
MS.gene50382.t1 AT5G42830 37.727 440 250 10 15 441 21 449 1.89e-85 269
MS.gene50382.t1 AT3G50270 37.856 457 255 14 2 441 4 448 1.15e-84 267
MS.gene50382.t1 AT5G07860 36.900 458 251 11 4 440 8 448 1.40e-78 251
MS.gene50382.t1 AT5G07850 37.773 458 253 13 4 441 8 453 1.11e-77 249
MS.gene50382.t1 AT5G38130 36.468 436 249 12 1 422 21 442 4.66e-75 242
MS.gene50382.t1 AT5G07870 37.580 471 244 15 4 441 8 461 1.68e-72 236
MS.gene50382.t1 AT2G39980 32.359 479 252 13 16 441 21 480 1.78e-65 218
MS.gene50382.t1 AT5G01210 33.333 417 219 11 66 442 73 470 1.49e-63 213
MS.gene50382.t1 AT3G50280 37.107 318 184 6 127 438 3 310 1.70e-61 202
MS.gene50382.t1 AT3G50290 40.433 277 146 7 122 390 4 269 2.80e-54 182
MS.gene50382.t1 AT5G23940 30.243 453 290 13 4 437 3 448 2.43e-52 183
MS.gene50382.t1 AT5G48930 26.484 438 288 17 7 428 5 424 3.78e-29 119
MS.gene50382.t1 AT3G29590 24.838 463 288 15 3 432 7 442 1.23e-22 100
MS.gene50382.t1 AT3G48720 27.714 350 217 14 55 393 61 385 2.25e-21 96.3
MS.gene50382.t1 AT2G19070 24.263 441 281 16 27 430 19 443 5.69e-21 95.5
MS.gene50382.t1 AT5G57840 27.009 448 265 20 7 422 5 422 1.63e-20 94.0
MS.gene50382.t1 AT3G03480 26.813 455 262 24 25 442 33 453 5.19e-20 92.4
MS.gene50382.t1 AT3G29670 23.094 446 297 14 3 422 8 433 5.80e-20 92.4
MS.gene50382.t1 AT5G41040 26.124 356 223 15 52 393 82 411 2.97e-19 90.1
MS.gene50382.t1 AT5G41040 26.124 356 223 15 52 393 66 395 3.39e-19 90.1
MS.gene50382.t1 AT5G61160 24.814 403 244 17 20 393 31 403 4.01e-18 86.7
MS.gene50382.t1 AT3G29635 24.682 393 257 14 24 392 29 406 9.14e-17 82.8
MS.gene50382.t1 AT5G39080 25.248 404 249 19 52 422 62 445 2.64e-16 81.3
MS.gene50382.t1 AT5G39090 28.467 274 166 13 135 392 140 399 8.13e-16 79.7
MS.gene50382.t1 AT2G25150 25.762 361 231 15 52 396 64 403 8.62e-16 79.7
MS.gene50382.t1 AT1G32910 26.741 359 223 16 53 393 63 399 4.13e-15 77.8
MS.gene50382.t1 AT5G16410 24.607 382 240 16 41 399 77 433 6.82e-15 77.0
MS.gene50382.t1 AT1G28680 24.336 452 255 20 15 432 18 416 1.61e-14 75.9
MS.gene50382.t1 AT1G78990 24.857 350 236 12 53 393 65 396 2.15e-14 75.5
MS.gene50382.t1 AT1G24430 24.819 415 264 17 41 441 45 425 8.74e-14 73.6
MS.gene50382.t1 AT1G03390 25.424 354 233 14 52 393 73 407 1.33e-13 72.8
MS.gene50382.t1 AT1G28680 25.076 331 203 13 15 325 18 323 1.46e-13 72.4
MS.gene50382.t1 AT3G30280 22.969 357 227 11 54 393 61 386 1.93e-13 72.4
MS.gene50382.t1 AT5G07080 25.172 290 165 12 56 322 71 331 2.08e-13 72.4
MS.gene50382.t1 AT5G17540 24.872 390 248 16 25 393 23 388 2.76e-13 72.0
MS.gene50382.t1 AT5G23970 24.541 436 278 17 4 423 3 403 6.21e-12 67.8
MS.gene50382.t1 AT1G31490 26.124 356 221 15 50 392 57 383 1.01e-11 67.0

Find 99 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATACGATTTCCTTTCTTTA+TGG 0.108680 4.4:+78902263 MS.gene50382:CDS
CTGTCTTCCTTACGAGATTT+TGG 0.135930 4.4:+78902917 MS.gene50382:CDS
TCTCCATGGTTCGATGTTTA+TGG 0.149887 4.4:+78902786 MS.gene50382:CDS
CCATTACCAGAATATTATTT+TGG 0.150661 4.4:+78902555 MS.gene50382:CDS
AATGAAAGTTGGGGAGTTAT+TGG 0.206917 4.4:+78902605 MS.gene50382:CDS
TAAGAAATCCAAGCTTTCTT+AGG 0.256333 4.4:+78902721 MS.gene50382:CDS
CTAGTTGACGGTATCTTTAT+TGG 0.259204 4.4:+78902093 MS.gene50382:CDS
CGTAGTGGATATAAAATAAA+TGG 0.273395 4.4:+78902843 MS.gene50382:CDS
AGTGTCTCAGCCGTTGAATT+TGG 0.274032 4.4:-78901807 None:intergenic
GCTTGCTTCAACCAACAAAA+AGG 0.287139 4.4:+78901753 MS.gene50382:CDS
ACACCTCACGTTGCATTACT+TGG 0.308599 4.4:-78902979 None:intergenic
GCATTTCCAAAATAATATTC+TGG 0.329273 4.4:-78902561 None:intergenic
GATGTTTATGGTAATGATTT+CGG 0.344240 4.4:+78902798 MS.gene50382:CDS
GTGTCTCAGCCGTTGAATTT+GGG 0.353976 4.4:-78901806 None:intergenic
ATCGTTTGATCACTTGATTT+TGG 0.361367 4.4:-78901700 None:intergenic
CCAAAATAATATTCTGGTAA+TGG 0.371844 4.4:-78902555 None:intergenic
GAGGTACGTAGGCCGGTTCC+AGG 0.380874 4.4:-78901984 None:intergenic
GGACTTGTTAACGTGTTTGC+TGG 0.385137 4.4:+78902864 MS.gene50382:CDS
TGTGGCATTTCATCAATTCA+TGG 0.389208 4.4:+78902154 MS.gene50382:CDS
ATGGGACTTTGAATACCATT+TGG 0.389462 4.4:-78902243 None:intergenic
GTATGGTTAAAATGAAAGTT+GGG 0.393088 4.4:+78902595 MS.gene50382:CDS
TGTATGGTTAAAATGAAAGT+TGG 0.395906 4.4:+78902594 MS.gene50382:CDS
GCCGGCAAGAGGTTGGAAAA+AGG 0.411903 4.4:-78901848 None:intergenic
TGTGAGTTAAAAGCGCTTGT+AGG 0.415532 4.4:-78902440 None:intergenic
AAATGCAGTTATAAGTTGTA+TGG 0.434670 4.4:+78902578 MS.gene50382:CDS
GGAAACCAACGTTCGATTGT+TGG 0.436462 4.4:-78902222 None:intergenic
TATGGTAATGATTTCGGATG+GGG 0.444025 4.4:+78902804 MS.gene50382:CDS
TTGGTTATCCTAAGAAAGCT+TGG 0.453190 4.4:-78902729 None:intergenic
TACTTGTGTTGCTGACATCC+TGG 0.455685 4.4:+78901966 MS.gene50382:CDS
TATTAAATACTTCTTCTTGT+GGG 0.457431 4.4:-78902497 None:intergenic
GTATTTAATAGAGTCGTGAT+AGG 0.460162 4.4:+78902510 MS.gene50382:CDS
GTGGCATTTCATCAATTCAT+GGG 0.460215 4.4:+78902155 MS.gene50382:CDS
ACCTGCATTGTTGCATATAA+CGG 0.463614 4.4:-78901914 None:intergenic
TGAATTTGGGTTGCTACCAC+CGG 0.474893 4.4:-78901793 None:intergenic
ATCGAACGTTGGTTTCCAAA+TGG 0.475255 4.4:+78902228 MS.gene50382:CDS
TGAAGAATCACTACGAGTCT+TGG 0.477110 4.4:+78902697 MS.gene50382:CDS
CTATTAAATACTTCTTCTTG+TGG 0.481064 4.4:-78902498 None:intergenic
GTGGGTCAAATTGTTTGAAA+CGG 0.484207 4.4:-78902479 None:intergenic
GTGAGTTAAAAGCGCTTGTA+GGG 0.487028 4.4:-78902439 None:intergenic
CGTAAGGAAGACAGACTTGA+AGG 0.487277 4.4:-78902908 None:intergenic
AATAGAGTCGTGATAGGAGT+TGG 0.491643 4.4:+78902516 MS.gene50382:CDS
TTCCGTTAGTTCAAGACGGC+CGG 0.494202 4.4:-78901866 None:intergenic
GGAGATCCCACGGAGTCAAA+TGG 0.501352 4.4:-78901723 None:intergenic
GACACAACATCAATGAATTG+AGG 0.505162 4.4:-78902957 None:intergenic
CATAAAGAAAGGAAATCGTA+TGG 0.514898 4.4:-78902262 None:intergenic
AGGCACGTCAAAACCATTGT+TGG 0.518095 4.4:+78902053 MS.gene50382:CDS
TTTATGGTAATGATTTCGGA+TGG 0.520125 4.4:+78902802 MS.gene50382:CDS
TGGTTGAAGCAAGCAGAAAA+CGG 0.521545 4.4:-78901744 None:intergenic
GGACCTTCTTTATCACCATC+CGG 0.521694 4.4:+78901774 MS.gene50382:CDS
TGTTGCTGACATCCTGGAAC+CGG 0.525755 4.4:+78901972 MS.gene50382:CDS
GGGGAGTTATTGGAGGAAGG+TGG 0.537462 4.4:+78902615 MS.gene50382:CDS
ACACAACATCAATGAATTGA+GGG 0.541053 4.4:-78902956 None:intergenic
TTAGAAAAGAATCACTCTGA+TGG 0.541983 4.4:+78902288 MS.gene50382:CDS
CAAGACGGCCGGCAAGAGGT+TGG 0.542100 4.4:-78901855 None:intergenic
AAATAATATTCTGGTAATGG+TGG 0.544089 4.4:-78902552 None:intergenic
GTTGGGGAGTTATTGGAGGA+AGG 0.545044 4.4:+78902612 MS.gene50382:CDS
GAAAGTTGGGGAGTTATTGG+AGG 0.545140 4.4:+78902608 MS.gene50382:CDS
GAGGAAGGTGGACTTTGTAA+AGG 0.546587 4.4:+78902627 MS.gene50382:CDS
TATCCACTACGAACTGCCAC+AGG 0.548923 4.4:-78902831 None:intergenic
TAGTCATTAGCAGTTCTCCA+TGG 0.550448 4.4:+78902772 MS.gene50382:CDS
TCCGTTATATGCAACAATGC+AGG 0.551070 4.4:+78901913 MS.gene50382:CDS
TTCAACCATGTTGTTGTCGA+CGG 0.555168 4.4:+78902123 MS.gene50382:CDS
TATCTCCACCGACACAATCA+AGG 0.559227 4.4:+78901675 MS.gene50382:CDS
TTATGGTAATGATTTCGGAT+GGG 0.560500 4.4:+78902803 MS.gene50382:CDS
TAGAAAAGAATCACTCTGAT+GGG 0.561164 4.4:+78902289 MS.gene50382:CDS
GGTAATGGTGGAACTAACCT+TGG 0.563399 4.4:-78902540 None:intergenic
TGCCGGCCGTCTTGAACTAA+CGG 0.576958 4.4:+78901864 MS.gene50382:CDS
ACAATTGGAGGTACGTAGGC+CGG 0.578807 4.4:-78901991 None:intergenic
GTGTTTGCTGGAATAGAAGA+AGG 0.582235 4.4:+78902876 MS.gene50382:CDS
AACCAACGTTCGATTGTTGG+AGG 0.582650 4.4:-78902219 None:intergenic
AGCAGAAAACGGAGATCCCA+CGG 0.583189 4.4:-78901733 None:intergenic
AACGATCCATTTGACTCCGT+GGG 0.584081 4.4:+78901717 MS.gene50382:CDS
TAGATTGTTCCACTTTACAA+AGG 0.584748 4.4:+78902356 MS.gene50382:CDS
CAAGTAACGGAGCTAGTTGA+CGG 0.586570 4.4:+78902081 MS.gene50382:CDS
ATTGTTGGCTGTTCAAGTAA+CGG 0.588367 4.4:+78902068 MS.gene50382:CDS
ATAAAGAAAGGAAATCGTAT+GGG 0.589028 4.4:-78902261 None:intergenic
ATTGCCTCCAAAATCTCGTA+AGG 0.590594 4.4:-78902924 None:intergenic
CCACCGGATGGTGATAAAGA+AGG 0.597188 4.4:-78901777 None:intergenic
AGTTCAAGACGGCCGGCAAG+AGG 0.599106 4.4:-78901859 None:intergenic
TGGTGCCTTGATTGTGTCGG+TGG 0.603114 4.4:-78901680 None:intergenic
TTGTGTCGGTGGAGATAACT+CGG 0.603689 4.4:-78901669 None:intergenic
TATGGTTAAAATGAAAGTTG+GGG 0.603920 4.4:+78902596 MS.gene50382:CDS
AAACGATCCATTTGACTCCG+TGG 0.610706 4.4:+78901716 MS.gene50382:CDS
AAGCCTGTGGCAGTTCGTAG+TGG 0.624075 4.4:+78902828 MS.gene50382:CDS
CTTGAACTAACGGAACACTT+AGG 0.631743 4.4:+78901874 MS.gene50382:CDS
GTTACTTGAACAGCCAACAA+TGG 0.633211 4.4:-78902066 None:intergenic
TGGTAGCAACCCAAATTCAA+CGG 0.637527 4.4:+78901797 MS.gene50382:CDS
AACCTCCAACAATCGAACGT+TGG 0.637858 4.4:+78902217 MS.gene50382:CDS
TTTCGGATGGGGAAAGCCTG+TGG 0.644016 4.4:+78902815 MS.gene50382:CDS
AGTGTTCCGTTAGTTCAAGA+CGG 0.647836 4.4:-78901870 None:intergenic
TTTGGGTTGCTACCACCGGA+TGG 0.657651 4.4:-78901789 None:intergenic
TGAATTGATGAAATGCCACA+AGG 0.668442 4.4:-78902151 None:intergenic
TTGTCGACGGAAAATCCTTG+TGG 0.668665 4.4:+78902136 MS.gene50382:CDS
GTCGGTGGAGATAACTCGGA+CGG 0.675224 4.4:-78901665 None:intergenic
TCGTGATAGGAGTTGGACCA+AGG 0.676348 4.4:+78902523 MS.gene50382:CDS
ATCGACAATTGGAGGTACGT+AGG 0.682001 4.4:-78901995 None:intergenic
TTACCATAAACATCGAACCA+TGG 0.683799 4.4:-78902789 None:intergenic
CCTTCTTTATCACCATCCGG+TGG 0.697565 4.4:+78901777 MS.gene50382:CDS
AAGTATTATCTGCTACAGCG+TGG 0.709809 4.4:-78901948 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAATACTTCTTCTTGT+GGG - chr4.4:78902500-78902519 None:intergenic 20.0%
!!! AATGACTAAGAAATTGTTTT+TGG - chr4.4:78902760-78902779 None:intergenic 20.0%
! AAATGCAGTTATAAGTTGTA+TGG + chr4.4:78902578-78902597 MS.gene50382:CDS 25.0%
! ATAAAGAAAGGAAATCGTAT+GGG - chr4.4:78902264-78902283 None:intergenic 25.0%
! CCAAAATAATATTCTGGTAA+TGG - chr4.4:78902558-78902577 None:intergenic 25.0%
! CTATTAAATACTTCTTCTTG+TGG - chr4.4:78902501-78902520 None:intergenic 25.0%
! GCATTTCCAAAATAATATTC+TGG - chr4.4:78902564-78902583 None:intergenic 25.0%
! GTATGGTTAAAATGAAAGTT+GGG + chr4.4:78902595-78902614 MS.gene50382:CDS 25.0%
! TATGGTTAAAATGAAAGTTG+GGG + chr4.4:78902596-78902615 MS.gene50382:CDS 25.0%
! TCAAAAATCAACAAAGAAGA+CGG + chr4.4:78902399-78902418 MS.gene50382:CDS 25.0%
! TGTATGGTTAAAATGAAAGT+TGG + chr4.4:78902594-78902613 MS.gene50382:CDS 25.0%
!! CCATTACCAGAATATTATTT+TGG + chr4.4:78902555-78902574 MS.gene50382:CDS 25.0%
!! GATGTTTATGGTAATGATTT+CGG + chr4.4:78902798-78902817 MS.gene50382:CDS 25.0%
!! GTAGTTTTTATCTCCAATAA+AGG - chr4.4:78902031-78902050 None:intergenic 25.0%
!! TTTTCTAACTCCATAAAGAA+AGG - chr4.4:78902276-78902295 None:intergenic 25.0%
!!! AAATAATATTCTGGTAATGG+TGG - chr4.4:78902555-78902574 None:intergenic 25.0%
!!! CGTAGTGGATATAAAATAAA+TGG + chr4.4:78902843-78902862 MS.gene50382:CDS 25.0%
AAAAATGAATCGACAATTGG+AGG - chr4.4:78902006-78902025 None:intergenic 30.0%
ACACAACATCAATGAATTGA+GGG - chr4.4:78902959-78902978 None:intergenic 30.0%
ATTGGAGATAAAAACTACGA+AGG + chr4.4:78902033-78902052 MS.gene50382:CDS 30.0%
CATAAAGAAAGGAAATCGTA+TGG - chr4.4:78902265-78902284 None:intergenic 30.0%
CATACGATTTCCTTTCTTTA+TGG + chr4.4:78902263-78902282 MS.gene50382:CDS 30.0%
GTATTTAATAGAGTCGTGAT+AGG + chr4.4:78902510-78902529 MS.gene50382:CDS 30.0%
TAAGAAATCCAAGCTTTCTT+AGG + chr4.4:78902721-78902740 MS.gene50382:CDS 30.0%
TAGAAAAGAATCACTCTGAT+GGG + chr4.4:78902289-78902308 MS.gene50382:CDS 30.0%
TAGATTGTTCCACTTTACAA+AGG + chr4.4:78902356-78902375 MS.gene50382:CDS 30.0%
TTAGAAAAGAATCACTCTGA+TGG + chr4.4:78902288-78902307 MS.gene50382:CDS 30.0%
! ATAAAGAAGGTCCTTTTTGT+TGG - chr4.4:78901767-78901786 None:intergenic 30.0%
! ATCGTTTGATCACTTGATTT+TGG - chr4.4:78901703-78901722 None:intergenic 30.0%
! TTATGGTAATGATTTCGGAT+GGG + chr4.4:78902803-78902822 MS.gene50382:CDS 30.0%
! TTTATGGTAATGATTTCGGA+TGG + chr4.4:78902802-78902821 MS.gene50382:CDS 30.0%
!! GATTCATTTTTCGCCTTTAT+TGG + chr4.4:78902015-78902034 MS.gene50382:CDS 30.0%
!! TTGTAGGGAAGATATTTTGA+TGG - chr4.4:78902427-78902446 None:intergenic 30.0%
!!! GAAATTGTTTTTGGCTATGT+TGG - chr4.4:78902751-78902770 None:intergenic 30.0%
AAGAGGTTGGAAAAAGGAAA+GGG - chr4.4:78901845-78901864 None:intergenic 35.0%
AATGAAAGTTGGGGAGTTAT+TGG + chr4.4:78902605-78902624 MS.gene50382:CDS 35.0%
ACCTGCATTGTTGCATATAA+CGG - chr4.4:78901917-78901936 None:intergenic 35.0%
ATGGGACTTTGAATACCATT+TGG - chr4.4:78902246-78902265 None:intergenic 35.0%
CTAGTTGACGGTATCTTTAT+TGG + chr4.4:78902093-78902112 MS.gene50382:CDS 35.0%
GACACAACATCAATGAATTG+AGG - chr4.4:78902960-78902979 None:intergenic 35.0%
GCGAAAAATGAATCGACAAT+TGG - chr4.4:78902009-78902028 None:intergenic 35.0%
GTGGCATTTCATCAATTCAT+GGG + chr4.4:78902155-78902174 MS.gene50382:CDS 35.0%
GTGGGTCAAATTGTTTGAAA+CGG - chr4.4:78902482-78902501 None:intergenic 35.0%
TGAATTGATGAAATGCCACA+AGG - chr4.4:78902154-78902173 None:intergenic 35.0%
TGTGGCATTTCATCAATTCA+TGG + chr4.4:78902154-78902173 MS.gene50382:CDS 35.0%
TTACCATAAACATCGAACCA+TGG - chr4.4:78902792-78902811 None:intergenic 35.0%
TTGGTTATCCTAAGAAAGCT+TGG - chr4.4:78902732-78902751 None:intergenic 35.0%
! ATTGTTGGCTGTTCAAGTAA+CGG + chr4.4:78902068-78902087 MS.gene50382:CDS 35.0%
! CGATTTTCTCCTTTGTAAAG+TGG - chr4.4:78902368-78902387 None:intergenic 35.0%
! TATGGTAATGATTTCGGATG+GGG + chr4.4:78902804-78902823 MS.gene50382:CDS 35.0%
!! AAGCGCTTTTAACTCACATT+TGG + chr4.4:78902445-78902464 MS.gene50382:CDS 35.0%
AAGTATTATCTGCTACAGCG+TGG - chr4.4:78901951-78901970 None:intergenic 40.0%
AATAGAGTCGTGATAGGAGT+TGG + chr4.4:78902516-78902535 MS.gene50382:CDS 40.0%
AGTGTTCCGTTAGTTCAAGA+CGG - chr4.4:78901873-78901892 None:intergenic 40.0%
ATTGCCTCCAAAATCTCGTA+AGG - chr4.4:78902927-78902946 None:intergenic 40.0%
CAAGAGGTTGGAAAAAGGAA+AGG - chr4.4:78901846-78901865 None:intergenic 40.0%
CTTGAACTAACGGAACACTT+AGG + chr4.4:78901874-78901893 MS.gene50382:CDS 40.0%
GCTTGCTTCAACCAACAAAA+AGG + chr4.4:78901753-78901772 MS.gene50382:CDS 40.0%
GTTACTTGAACAGCCAACAA+TGG - chr4.4:78902069-78902088 None:intergenic 40.0%
TAGTCATTAGCAGTTCTCCA+TGG + chr4.4:78902772-78902791 MS.gene50382:CDS 40.0%
TCCGTTATATGCAACAATGC+AGG + chr4.4:78901913-78901932 MS.gene50382:CDS 40.0%
TCTCCATGGTTCGATGTTTA+TGG + chr4.4:78902786-78902805 MS.gene50382:CDS 40.0%
TGAAGAATCACTACGAGTCT+TGG + chr4.4:78902697-78902716 MS.gene50382:CDS 40.0%
TGGTAGCAACCCAAATTCAA+CGG + chr4.4:78901797-78901816 MS.gene50382:CDS 40.0%
TGGTTGAAGCAAGCAGAAAA+CGG - chr4.4:78901747-78901766 None:intergenic 40.0%
TTCAACCATGTTGTTGTCGA+CGG + chr4.4:78902123-78902142 MS.gene50382:CDS 40.0%
! ATTTTCCGTCGACAACAACA+TGG - chr4.4:78902131-78902150 None:intergenic 40.0%
! CTGTCTTCCTTACGAGATTT+TGG + chr4.4:78902917-78902936 MS.gene50382:CDS 40.0%
! GTGTTTGCTGGAATAGAAGA+AGG + chr4.4:78902876-78902895 MS.gene50382:CDS 40.0%
! TCTTCCTTACGAGATTTTGG+AGG + chr4.4:78902920-78902939 MS.gene50382:CDS 40.0%
!! ATCGAACGTTGGTTTCCAAA+TGG + chr4.4:78902228-78902247 MS.gene50382:CDS 40.0%
!! GTGAGTTAAAAGCGCTTGTA+GGG - chr4.4:78902442-78902461 None:intergenic 40.0%
!! TACGAGATTTTGGAGGCAAT+GGG + chr4.4:78902927-78902946 MS.gene50382:CDS 40.0%
!! TGTGAGTTAAAAGCGCTTGT+AGG - chr4.4:78902443-78902462 None:intergenic 40.0%
!! TTACGAGATTTTGGAGGCAA+TGG + chr4.4:78902926-78902945 MS.gene50382:CDS 40.0%
!!! TTTTGGTGCCTTGATTGTGT+CGG - chr4.4:78901686-78901705 None:intergenic 40.0%
AAACGATCCATTTGACTCCG+TGG + chr4.4:78901716-78901735 MS.gene50382:CDS 45.0%
AACCAACGTTCGATTGTTGG+AGG - chr4.4:78902222-78902241 None:intergenic 45.0%
AACCTCCAACAATCGAACGT+TGG + chr4.4:78902217-78902236 MS.gene50382:CDS 45.0%
AACGATCCATTTGACTCCGT+GGG + chr4.4:78901717-78901736 MS.gene50382:CDS 45.0%
AGGCACGTCAAAACCATTGT+TGG + chr4.4:78902053-78902072 MS.gene50382:CDS 45.0%
AGTGTCTCAGCCGTTGAATT+TGG - chr4.4:78901810-78901829 None:intergenic 45.0%
CGTAAGGAAGACAGACTTGA+AGG - chr4.4:78902911-78902930 None:intergenic 45.0%
GAAAGTTGGGGAGTTATTGG+AGG + chr4.4:78902608-78902627 MS.gene50382:CDS 45.0%
GGAAACCAACGTTCGATTGT+TGG - chr4.4:78902225-78902244 None:intergenic 45.0%
GGACCTTCTTTATCACCATC+CGG + chr4.4:78901774-78901793 MS.gene50382:CDS 45.0%
GTGTCTCAGCCGTTGAATTT+GGG - chr4.4:78901809-78901828 None:intergenic 45.0%
TATCTCCACCGACACAATCA+AGG + chr4.4:78901675-78901694 MS.gene50382:CDS 45.0%
TTGGAAAAAGGAAAGGGCAG+AGG - chr4.4:78901839-78901858 None:intergenic 45.0%
TTGTCGACGGAAAATCCTTG+TGG + chr4.4:78902136-78902155 MS.gene50382:CDS 45.0%
! ATCGACAATTGGAGGTACGT+AGG - chr4.4:78901998-78902017 None:intergenic 45.0%
! CAAGTAACGGAGCTAGTTGA+CGG + chr4.4:78902081-78902100 MS.gene50382:CDS 45.0%
! GAGGAAGGTGGACTTTGTAA+AGG + chr4.4:78902627-78902646 MS.gene50382:CDS 45.0%
! GGTAATGGTGGAACTAACCT+TGG - chr4.4:78902543-78902562 None:intergenic 45.0%
! TACTTGTGTTGCTGACATCC+TGG + chr4.4:78901966-78901985 MS.gene50382:CDS 45.0%
! TCCTTTTTCCAACCTCTTGC+CGG + chr4.4:78901847-78901866 MS.gene50382:CDS 45.0%
! TTGTGTCGGTGGAGATAACT+CGG - chr4.4:78901672-78901691 None:intergenic 45.0%
!! GGACTTGTTAACGTGTTTGC+TGG + chr4.4:78902864-78902883 MS.gene50382:CDS 45.0%
!! TGAATTTGGGTTGCTACCAC+CGG - chr4.4:78901796-78901815 None:intergenic 45.0%
AGCAGAAAACGGAGATCCCA+CGG - chr4.4:78901736-78901755 None:intergenic 50.0%
CCACCGGATGGTGATAAAGA+AGG - chr4.4:78901780-78901799 None:intergenic 50.0%
CCTTCTTTATCACCATCCGG+TGG + chr4.4:78901777-78901796 MS.gene50382:CDS 50.0%
GTTGGGGAGTTATTGGAGGA+AGG + chr4.4:78902612-78902631 MS.gene50382:CDS 50.0%
TATCCACTACGAACTGCCAC+AGG - chr4.4:78902834-78902853 None:intergenic 50.0%
TCGTGATAGGAGTTGGACCA+AGG + chr4.4:78902523-78902542 MS.gene50382:CDS 50.0%
TGTTGCTGACATCCTGGAAC+CGG + chr4.4:78901972-78901991 MS.gene50382:CDS 50.0%
TTCCGTTAGTTCAAGACGGC+CGG - chr4.4:78901869-78901888 None:intergenic 50.0%
! ACAATTGGAGGTACGTAGGC+CGG - chr4.4:78901994-78902013 None:intergenic 50.0%
AAGCCTGTGGCAGTTCGTAG+TGG + chr4.4:78902828-78902847 MS.gene50382:CDS 55.0%
GCCGGCAAGAGGTTGGAAAA+AGG - chr4.4:78901851-78901870 None:intergenic 55.0%
GGAGATCCCACGGAGTCAAA+TGG - chr4.4:78901726-78901745 None:intergenic 55.0%
GGGGAGTTATTGGAGGAAGG+TGG + chr4.4:78902615-78902634 MS.gene50382:CDS 55.0%
GTCGGTGGAGATAACTCGGA+CGG - chr4.4:78901668-78901687 None:intergenic 55.0%
TGCCGGCCGTCTTGAACTAA+CGG + chr4.4:78901864-78901883 MS.gene50382:CDS 55.0%
TGGTGCCTTGATTGTGTCGG+TGG - chr4.4:78901683-78901702 None:intergenic 55.0%
TTTCGGATGGGGAAAGCCTG+TGG + chr4.4:78902815-78902834 MS.gene50382:CDS 55.0%
!! TTTGGGTTGCTACCACCGGA+TGG - chr4.4:78901792-78901811 None:intergenic 55.0%
AGTTCAAGACGGCCGGCAAG+AGG - chr4.4:78901862-78901881 None:intergenic 60.0%
CAAGACGGCCGGCAAGAGGT+TGG - chr4.4:78901858-78901877 None:intergenic 65.0%
! GAGGTACGTAGGCCGGTTCC+AGG - chr4.4:78901987-78902006 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 78901658 78902986 78901658 ID=MS.gene50382
chr4.4 mRNA 78901658 78902986 78901658 ID=MS.gene50382.t1;Parent=MS.gene50382
chr4.4 exon 78901658 78902986 78901658 ID=MS.gene50382.t1.exon1;Parent=MS.gene50382.t1
chr4.4 CDS 78901658 78902986 78901658 ID=cds.MS.gene50382.t1;Parent=MS.gene50382.t1
Gene Sequence

>MS.gene50382

ATGGGTGCCGTCCGAGTTATCTCCACCGACACAATCAAGGCACCAAAATCAAGTGATCAAACGATCCATTTGACTCCGTGGGATCTCCGTTTTCTGCTTGCTTCAACCAACAAAAAGGACCTTCTTTATCACCATCCGGTGGTAGCAACCCAAATTCAACGGCTGAGACACTCTCTATCCTCTGCCCTTTCCTTTTTCCAACCTCTTGCCGGCCGTCTTGAACTAACGGAACACTTAGGCAACACTGTCTCGTGTTCCGTTATATGCAACAATGCAGGTGTACTCTTTATCCACGCTGTAGCAGATAATACTTGTGTTGCTGACATCCTGGAACCGGCCTACGTACCTCCAATTGTCGATTCATTTTTCGCCTTTATTGGAGATAAAAACTACGAAGGCACGTCAAAACCATTGTTGGCTGTTCAAGTAACGGAGCTAGTTGACGGTATCTTTATTGGCTGCACATTCAACCATGTTGTTGTCGACGGAAAATCCTTGTGGCATTTCATCAATTCATGGGCTGAAATCTCACGTAATTGTTGTCATCATCAAATATCTAAACCTCCAACAATCGAACGTTGGTTTCCAAATGGTATTCAAAGTCCCATACGATTTCCTTTCTTTATGGAGTTAGAAAAGAATCACTCTGATGGGTTGACTATTTCATCATCTAATGATGAAAAACTTTGTTTATTAAATAGATTGTTCCACTTTACAAAGGAGAAAATCGTGCAACTTAAATCAAAAATCAACAAAGAAGACGGTACCATCAAAATATCTTCCCTACAAGCGCTTTTAACTCACATTTGGTGTTATGTGATCCGTTTCAAACAATTTGACCCACAAGAAGAAGTATTTAATAGAGTCGTGATAGGAGTTGGACCAAGGTTAGTTCCACCATTACCAGAATATTATTTTGGAAATGCAGTTATAAGTTGTATGGTTAAAATGAAAGTTGGGGAGTTATTGGAGGAAGGTGGACTTTGTAAAGGTGCTTGTGAGATGAACAAGTTAATTGCTTCACACACTGATGAGAAGTTGAAGAATCACTACGAGTCTTGGTTAAGAAATCCAAGCTTTCTTAGGATAACCAACATAGCCAAAAACAATTTCTTAGTCATTAGCAGTTCTCCATGGTTCGATGTTTATGGTAATGATTTCGGATGGGGAAAGCCTGTGGCAGTTCGTAGTGGATATAAAATAAATGGACTTGTTAACGTGTTTGCTGGAATAGAAGAAGGTAGTATTGACCTTCAAGTCTGTCTTCCTTACGAGATTTTGGAGGCAATGGGAAGTGACCCTCAATTCATTGATGTTGTGTCCAAGTAA
Protein sequence

>MS.gene50382.t1

MGAVRVISTDTIKAPKSSDQTIHLTPWDLRFLLASTNKKDLLYHHPVVATQIQRLRHSLSSALSFFQPLAGRLELTEHLGNTVSCSVICNNAGVLFIHAVADNTCVADILEPAYVPPIVDSFFAFIGDKNYEGTSKPLLAVQVTELVDGIFIGCTFNHVVVDGKSLWHFINSWAEISRNCCHHQISKPPTIERWFPNGIQSPIRFPFFMELEKNHSDGLTISSSNDEKLCLLNRLFHFTKEKIVQLKSKINKEDGTIKISSLQALLTHIWCYVIRFKQFDPQEEVFNRVVIGVGPRLVPPLPEYYFGNAVISCMVKMKVGELLEEGGLCKGACEMNKLIASHTDEKLKNHYESWLRNPSFLRITNIAKNNFLVISSSPWFDVYGNDFGWGKPVAVRSGYKINGLVNVFAGIEEGSIDLQVCLPYEILEAMGSDPQFIDVVSK