AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene013787

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013787.t1 MTR_3g088420 94.226 433 25 0 1 433 1 433 0.0 857
MS.gene013787.t1 MTR_3g026310 72.098 448 110 3 1 433 1 448 0.0 658
MS.gene013787.t1 MTR_4g022800 68.552 442 130 3 1 433 1 442 0.0 612
MS.gene013787.t1 MTR_4g022670 67.347 441 136 2 1 433 1 441 0.0 609
MS.gene013787.t1 MTR_4g022825 67.273 440 137 1 1 433 1 440 0.0 605
MS.gene013787.t1 MTR_4g022720 68.046 435 137 2 1 433 1 435 0.0 600
MS.gene013787.t1 MTR_7g026860 66.440 441 137 3 1 433 1 438 0.0 585
MS.gene013787.t1 MTR_8g041770 61.486 444 157 6 1 433 1 441 0.0 533
MS.gene013787.t1 MTR_0190s0020 61.036 444 160 6 1 433 1 442 0.0 530
MS.gene013787.t1 MTR_4g022760 60.091 441 124 3 1 433 1 397 0.0 517
MS.gene013787.t1 MTR_4g094842 56.319 451 172 9 1 431 1 446 1.47e-167 478
MS.gene013787.t1 MTR_4g094835 54.018 448 181 10 1 429 1 442 1.12e-162 466
MS.gene013787.t1 MTR_4g094828 51.648 455 195 11 1 434 1 451 3.39e-152 439
MS.gene013787.t1 MTR_6g016815 54.734 433 130 6 1 433 15 381 4.24e-151 434
MS.gene013787.t1 MTR_8g079710 53.915 447 182 12 1 429 1 441 1.48e-148 430
MS.gene013787.t1 MTR_4g094838 51.225 449 175 8 4 429 1 428 2.54e-147 426
MS.gene013787.t1 MTR_4g022290 50.998 451 191 11 1 430 1 442 1.45e-144 419
MS.gene013787.t1 MTR_4g094832 54.521 376 150 7 69 429 57 426 1.65e-134 394
MS.gene013787.t1 MTR_3g101900 38.889 432 241 10 19 434 25 449 1.73e-92 286
MS.gene013787.t1 MTR_8g040780 62.780 223 79 2 210 428 5 227 2.04e-92 279
MS.gene013787.t1 MTR_8g040760 65.094 212 68 2 1 206 1 212 2.86e-90 273
MS.gene013787.t1 MTR_3g102020 37.701 435 246 10 19 434 24 452 4.46e-85 268
MS.gene013787.t1 MTR_3g101930 37.296 429 245 9 24 432 30 454 8.94e-85 267
MS.gene013787.t1 MTR_3g101970 36.961 441 240 11 11 434 19 438 1.14e-82 261
MS.gene013787.t1 MTR_3g102010 37.529 429 244 10 24 434 30 452 9.49e-82 259
MS.gene013787.t1 MTR_3g101960 36.343 432 243 11 19 428 24 445 5.21e-79 252
MS.gene013787.t1 MTR_7g026850 71.613 155 44 0 29 183 12 166 1.57e-77 239
MS.gene013787.t1 MTR_3g070170 30.962 478 267 13 2 433 10 470 9.54e-61 205
MS.gene013787.t1 MTR_6g034485 71.774 124 34 1 189 312 3 125 1.69e-57 185
MS.gene013787.t1 MTR_7g026770 49.362 235 77 4 109 322 80 293 1.77e-57 191
MS.gene013787.t1 MTR_5g081960 31.172 478 250 12 19 429 17 482 4.05e-57 196
MS.gene013787.t1 MTR_4g015180 32.985 479 242 16 20 433 23 487 1.41e-55 191
MS.gene013787.t1 MTR_4g094825 55.689 167 67 4 16 176 83 248 4.21e-52 180
MS.gene013787.t1 MTR_4g094825 51.656 151 60 2 285 433 252 391 1.30e-41 152
MS.gene013787.t1 MTR_6g045553 29.741 464 282 12 4 429 14 471 9.13e-50 176
MS.gene013787.t1 MTR_6g034495 62.774 137 41 3 49 180 33 164 2.57e-47 160
MS.gene013787.t1 MTR_3g102030 33.225 307 171 6 87 389 44 320 1.67e-44 157
MS.gene013787.t1 MTR_7g026830 72.619 84 23 0 82 165 14 97 4.20e-38 134
MS.gene013787.t1 MTR_8g096530 28.261 414 228 15 53 426 57 441 6.87e-32 126
MS.gene013787.t1 MTR_3g026950 27.322 366 222 12 52 385 51 404 1.84e-29 119
MS.gene013787.t1 MTR_7g026800 44.375 160 58 4 215 372 30 160 1.37e-28 111
MS.gene013787.t1 MTR_3g098980 27.066 351 209 11 55 385 66 389 1.43e-28 117
MS.gene013787.t1 MTR_8g075610 27.013 385 246 15 55 422 47 413 3.42e-28 115
MS.gene013787.t1 MTR_4g007540 24.775 444 279 18 4 417 3 421 4.57e-28 115
MS.gene013787.t1 MTR_4g022780 43.558 163 61 4 214 374 30 163 4.93e-28 109
MS.gene013787.t1 MTR_2g105330 25.330 379 249 12 55 415 56 418 3.25e-27 113
MS.gene013787.t1 MTR_7g026780 50.909 110 30 2 324 433 26 111 2.63e-26 102
MS.gene013787.t1 MTR_2g064495 25.066 379 250 12 55 415 56 418 2.83e-26 110
MS.gene013787.t1 MTR_3g049150 28.421 380 223 17 42 384 32 399 3.68e-25 107
MS.gene013787.t1 MTR_4g013050 24.860 358 207 9 49 385 47 363 5.81e-23 100
MS.gene013787.t1 MTR_1g081660 25.773 388 240 15 24 385 31 396 9.10e-21 94.7
MS.gene013787.t1 MTR_4g097090 24.554 448 285 19 21 432 27 457 9.29e-21 94.7
MS.gene013787.t1 MTR_7g027175 23.410 393 272 11 50 431 63 437 7.75e-20 91.7
MS.gene013787.t1 MTR_1g040195 26.841 421 259 18 25 427 23 412 1.18e-18 88.2
MS.gene013787.t1 MTR_2g089670 26.601 406 238 15 21 384 42 429 1.31e-18 88.2
MS.gene013787.t1 MTR_7g085930 25.648 386 220 13 52 390 56 421 1.93e-18 87.8
MS.gene013787.t1 MTR_7g014330 23.844 411 254 15 19 388 21 413 2.94e-18 87.0
MS.gene013787.t1 MTR_7g026870 48.718 78 40 0 266 343 74 151 3.48e-18 81.6
MS.gene013787.t1 MTR_2g089755 25.346 434 260 19 24 409 37 454 5.37e-18 86.3
MS.gene013787.t1 MTR_5g076700 22.851 442 280 16 25 429 24 441 7.13e-18 85.9
MS.gene013787.t1 MTR_6g015320 24.297 391 259 10 24 387 29 409 1.21e-17 85.1
MS.gene013787.t1 MTR_6g015830 23.756 442 289 12 15 422 25 452 4.54e-17 83.6
MS.gene013787.t1 MTR_7g026880 63.462 52 19 0 382 433 2 53 6.60e-17 74.7
MS.gene013787.t1 MTR_3g087640 25.397 441 276 16 24 420 31 462 3.75e-16 80.9
MS.gene013787.t1 MTR_2g089650 23.709 426 245 16 38 409 43 442 1.42e-15 79.0
MS.gene013787.t1 MTR_8g012750 25.185 405 251 12 24 384 26 422 1.50e-15 79.0
MS.gene013787.t1 MTR_1g040175 27.660 282 174 11 50 317 59 324 1.68e-15 78.6
MS.gene013787.t1 MTR_7g085990 25.806 310 196 9 52 337 52 351 2.06e-15 78.6
MS.gene013787.t1 MTR_7g114470 23.570 437 284 15 25 429 25 443 2.57e-15 78.2
MS.gene013787.t1 MTR_7g114550 21.622 444 282 19 25 429 24 440 3.43e-15 77.8
MS.gene013787.t1 MTR_7g014200 23.659 410 257 13 22 388 28 424 3.77e-15 77.8
MS.gene013787.t1 MTR_3g086020 25.620 363 223 11 55 406 61 387 1.05e-14 75.9
MS.gene013787.t1 MTR_1g053315 25.323 387 247 18 55 416 78 447 1.23e-14 75.9
MS.gene013787.t1 MTR_7g015860 25.708 424 231 19 15 385 21 413 2.23e-14 75.1
MS.gene013787.t1 MTR_7g014620 24.709 429 256 17 24 414 15 414 4.32e-14 74.3
MS.gene013787.t1 MTR_4g055170 25.413 303 196 11 55 343 69 355 5.65e-14 73.9
MS.gene013787.t1 MTR_2g089765 23.697 422 255 14 15 384 28 434 6.29e-14 73.9
MS.gene013787.t1 MTR_7g086000 29.615 260 161 12 58 302 63 315 3.27e-13 71.6
MS.gene013787.t1 MTR_3g465980 25.993 277 177 9 55 316 68 331 7.22e-13 70.5
MS.gene013787.t1 MTR_5g090810 24.372 398 236 17 32 385 36 412 2.53e-12 68.9
MS.gene013787.t1 MTR_3g049140 25.541 231 148 9 204 414 6 232 7.65e-12 65.5
MS.gene013787.t1 MTR_5g090830 24.181 397 238 16 32 385 36 412 8.57e-12 67.4
MS.gene013787.t1 MTR_3g465990 25.725 276 179 11 55 316 68 331 2.50e-11 65.9
MS.gene013787.t1 MTR_7g014360 25.475 263 175 10 138 388 90 343 4.35e-11 64.7
MS.gene013787.t1 MTR_3g112180 23.907 389 256 15 52 420 58 426 8.47e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013787.t1 AT5G67160 40.183 438 237 11 6 434 12 433 1.30e-97 299
MS.gene013787.t1 AT5G67150 38.546 454 252 11 1 434 1 447 2.18e-96 296
MS.gene013787.t1 AT3G50300 42.056 428 230 11 1 414 1 424 1.11e-95 295
MS.gene013787.t1 AT3G50280 37.978 445 256 11 1 430 1 440 2.87e-91 283
MS.gene013787.t1 AT3G50270 39.220 436 249 11 12 434 17 449 7.46e-90 280
MS.gene013787.t1 AT5G38130 40.233 430 233 12 1 414 21 442 2.99e-87 273
MS.gene013787.t1 AT5G42830 36.636 434 257 10 15 434 21 450 8.53e-84 264
MS.gene013787.t1 AT5G07860 36.807 451 257 14 2 432 6 448 1.03e-77 249
MS.gene013787.t1 AT5G07870 36.364 462 258 14 2 433 6 461 2.05e-76 246
MS.gene013787.t1 AT5G07850 34.934 458 262 15 2 433 6 453 3.70e-69 226
MS.gene013787.t1 AT3G50280 38.462 312 180 6 127 430 3 310 1.84e-64 210
MS.gene013787.t1 AT2G39980 32.505 483 255 13 11 433 9 480 2.69e-64 214
MS.gene013787.t1 AT3G50290 42.222 270 143 5 122 382 4 269 1.50e-60 198
MS.gene013787.t1 AT5G01210 32.439 410 222 11 66 433 73 469 5.46e-58 197
MS.gene013787.t1 AT5G23940 30.599 451 283 12 4 429 3 448 2.94e-55 191
MS.gene013787.t1 AT3G48720 28.152 341 219 10 55 385 61 385 1.70e-28 116
MS.gene013787.t1 AT5G41040 26.513 347 225 10 52 385 82 411 3.31e-26 110
MS.gene013787.t1 AT5G41040 26.513 347 225 10 52 385 66 395 3.44e-26 110
MS.gene013787.t1 AT5G48930 27.072 362 219 15 53 391 54 393 4.36e-26 110
MS.gene013787.t1 AT5G57840 28.030 396 231 18 49 414 51 422 2.97e-22 99.0
MS.gene013787.t1 AT2G19070 25.776 419 254 19 50 431 53 451 7.17e-22 97.8
MS.gene013787.t1 AT5G63560 25.429 350 213 12 55 385 61 381 2.30e-21 96.3
MS.gene013787.t1 AT3G29590 24.053 449 303 12 2 424 6 442 4.70e-21 95.5
MS.gene013787.t1 AT3G26040 26.290 407 246 17 51 434 55 430 1.36e-20 94.0
MS.gene013787.t1 AT3G29635 25.714 420 273 14 24 414 29 438 7.21e-20 92.0
MS.gene013787.t1 AT1G28680 24.062 453 284 19 1 427 1 419 1.51e-18 87.8
MS.gene013787.t1 AT1G24420 23.556 450 301 16 4 434 5 430 8.53e-18 85.5
MS.gene013787.t1 AT1G24420 23.556 450 301 16 4 434 5 430 8.53e-18 85.5
MS.gene013787.t1 AT3G29670 22.477 436 286 15 20 422 25 441 1.65e-17 84.7
MS.gene013787.t1 AT2G40230 27.136 398 243 17 11 389 22 391 1.65e-17 84.7
MS.gene013787.t1 AT5G39050 27.119 354 186 15 122 420 132 468 1.18e-16 82.4
MS.gene013787.t1 AT1G28680 24.523 367 229 17 1 343 1 343 6.24e-16 79.7
MS.gene013787.t1 AT1G24430 25.397 378 216 17 54 406 61 397 4.12e-15 77.4
MS.gene013787.t1 AT5G39090 28.571 385 217 18 42 394 56 414 7.92e-15 76.6
MS.gene013787.t1 AT1G03390 23.587 407 257 16 42 415 59 444 2.07e-14 75.5
MS.gene013787.t1 AT5G23970 22.637 455 283 16 4 429 3 417 7.30e-14 73.6
MS.gene013787.t1 AT5G61160 22.251 391 267 13 22 385 23 403 1.35e-13 72.8
MS.gene013787.t1 AT1G78990 25.706 354 220 15 53 385 65 396 2.91e-13 71.6
MS.gene013787.t1 AT1G27620 23.360 381 262 13 55 422 58 421 8.33e-13 70.5
MS.gene013787.t1 AT3G29680 23.604 394 251 12 24 385 25 400 1.12e-12 70.1
MS.gene013787.t1 AT5G16410 24.416 385 229 16 32 385 66 419 2.92e-12 68.9
MS.gene013787.t1 AT5G47950 23.714 350 217 15 50 385 56 369 4.41e-12 68.2

Find 91 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCCGTGGTTTGATGTTTA+TGG 0.126200 3.4:+81533334 MS.gene013787:CDS
CCATTGCCTGAAGATTATTT+TGG 0.195668 3.4:+81533103 MS.gene013787:CDS
ATTGGCTAATGTATTATTAT+TGG 0.203749 3.4:-81533305 None:intergenic
GTGTCTTCCTCACAAGATTT+TGG 0.229479 3.4:+81533465 MS.gene013787:CDS
ACTAATTGCACCCACCAAAA+AGG 0.252147 3.4:+81532325 MS.gene013787:CDS
GATGTTTATGGTAATGATTT+TGG 0.308658 3.4:+81533346 MS.gene013787:CDS
ACGTTCTAGCGTTATGAGTT+TGG 0.324935 3.4:-81532783 None:intergenic
GCCTGCAAGTGGTGGGAAAA+AGG 0.338259 3.4:-81532420 None:intergenic
TTCAGATGTTGAATTTGATT+TGG 0.376205 3.4:-81532374 None:intergenic
GTGGGCTTGGTAAAGGTGCT+TGG 0.384077 3.4:+81533182 MS.gene013787:CDS
ACACGAAGAAGTTTATTATA+TGG 0.390132 3.4:+81533048 MS.gene013787:CDS
TTCGTGTGGGTGAAATTGTT+TGG 0.393408 3.4:-81533032 None:intergenic
GCATTTCCAAAATAATCTTC+AGG 0.400923 3.4:-81533109 None:intergenic
AATGAAAGCAGGACAGTTGT+TGG 0.413215 3.4:+81533153 MS.gene013787:CDS
AATTTGGTGACTTGATTGTA+TGG 0.415203 3.4:-81532256 None:intergenic
GGAATGATTACTGTATTTGC+TGG 0.415451 3.4:+81533412 MS.gene013787:CDS
TTGTATGGGTAGAGAGGACT+TGG 0.418563 3.4:-81532241 None:intergenic
AAACCACGGGGAATTGCTAT+TGG 0.430657 3.4:-81533323 None:intergenic
GGGAAATGACCATGACTTCA+TGG 0.444511 3.4:+81533495 MS.gene013787:CDS
TGGAAAAGAAGAAGGTAGTA+TGG 0.447023 3.4:+81533432 MS.gene013787:CDS
GAAATGCTGTTGAAGTTTGT+AGG 0.451612 3.4:+81533125 MS.gene013787:CDS
TTAGTTGACGGCATCTTCAT+TGG 0.458413 3.4:+81532665 MS.gene013787:CDS
TTGAAAGCCAACATGGAGAT+TGG 0.465372 3.4:+81532947 MS.gene013787:CDS
CGCAGTGGAAATAAAATAAG+TGG 0.469980 3.4:+81533391 MS.gene013787:CDS
TGAAGAATCACTACGAGTCT+TGG 0.474671 3.4:+81533245 MS.gene013787:CDS
CCAAAATAATCTTCAGGCAA+TGG 0.482695 3.4:-81533103 None:intergenic
TGGGCTTGGTAAAGGTGCTT+GGG 0.485107 3.4:+81533183 MS.gene013787:CDS
ATTTAACTCCATGGGATCTC+CGG 0.488953 3.4:+81532297 MS.gene013787:CDS
GAAGATGGTGGGCTTGGTAA+AGG 0.490096 3.4:+81533175 MS.gene013787:CDS
ACCTTGATCTAGCTCCTATC+GGG 0.496130 3.4:-81533072 None:intergenic
AACCTTGATCTAGCTCCTAT+CGG 0.500296 3.4:-81533073 None:intergenic
GCATGTGACGGAACATGAAA+TGG 0.505364 3.4:-81532474 None:intergenic
AATCACTACGAGTCTTGGTT+GGG 0.505413 3.4:+81533250 MS.gene013787:CDS
GTTGGTACCAATCTCCATGT+TGG 0.507485 3.4:-81532954 None:intergenic
GAATCACTACGAGTCTTGGT+TGG 0.508104 3.4:+81533249 MS.gene013787:CDS
TTTAACCACACGGTTGTTGA+CGG 0.510798 3.4:+81532695 MS.gene013787:CDS
TCGAGACGGCCTGCAAGTGG+TGG 0.521804 3.4:-81532428 None:intergenic
GCAGGACAGTTGTTGGAAGA+TGG 0.523872 3.4:+81533160 MS.gene013787:CDS
TATAGTGAAAGGAAATCGTA+TGG 0.523888 3.4:-81532831 None:intergenic
TACCATAAACATCAAACCAC+GGG 0.529973 3.4:-81533336 None:intergenic
TTTCCACTGCGAATTGCCAC+AGG 0.532396 3.4:-81533379 None:intergenic
GACAGTTGTTGGAAGATGGT+GGG 0.539492 3.4:+81533164 MS.gene013787:CDS
TGTGATCTGTTATTTCGAGA+CGG 0.540547 3.4:-81532442 None:intergenic
AAATGGAATAACCTCTCTGA+TGG 0.540722 3.4:-81532896 None:intergenic
AACTCATAACGCTAGAACGT+TGG 0.541457 3.4:+81532786 MS.gene013787:CDS
AATTGCGTAGGTGACATACT+TGG 0.547512 3.4:+81532539 MS.gene013787:CDS
TGTAGGGTTACAATGAAAGC+AGG 0.550470 3.4:+81533142 MS.gene013787:CDS
TTGTTGGAAGATGGTGGGCT+TGG 0.553654 3.4:+81533169 MS.gene013787:CDS
ACCAGCATTGTTGCATGTGA+CGG 0.553807 3.4:-81532486 None:intergenic
AAATGCTGTTGAAGTTTGTA+GGG 0.553867 3.4:+81533126 MS.gene013787:CDS
TCCCGATAGGAGCTAGATCA+AGG 0.559796 3.4:+81533071 MS.gene013787:CDS
GTATTTGCTGGAAAAGAAGA+AGG 0.567059 3.4:+81533424 MS.gene013787:CDS
AAATAATCTTCAGGCAATGG+TGG 0.571644 3.4:-81533100 None:intergenic
GGACAGTTGTTGGAAGATGG+TGG 0.574775 3.4:+81533163 MS.gene013787:CDS
AACGGATCACAGAGCGCCAT+AGG 0.575889 3.4:-81533009 None:intergenic
ACTGAAATTGAAAGCCAACA+TGG 0.579416 3.4:+81532940 MS.gene013787:CDS
ATTTCGAGACGGCCTGCAAG+TGG 0.589823 3.4:-81532431 None:intergenic
TGGGTGCAATTAGTAGGTAC+CGG 0.591093 3.4:-81532316 None:intergenic
GTTTATTATATGGTCCCGAT+AGG 0.594388 3.4:+81533058 MS.gene013787:CDS
ATATAATAAACTTCTTCGTG+TGG 0.595960 3.4:-81533046 None:intergenic
AATTGTTCTCCGCTGCGGCA+TGG 0.596421 3.4:-81532520 None:intergenic
TGAATTTGATTTGGCACTAG+AGG 0.597459 3.4:-81532365 None:intergenic
TATAATAAACTTCTTCGTGT+GGG 0.598129 3.4:-81533045 None:intergenic
TCCGTCACATGCAACAATGC+TGG 0.599979 3.4:+81532485 MS.gene013787:CDS
ACACAACATCCATGAAGTCA+TGG 0.605152 3.4:-81533504 None:intergenic
ATCGACGATGGGAGGAACGT+AGG 0.605346 3.4:-81532567 None:intergenic
AGTAGGTACCGGAGATCCCA+TGG 0.606618 3.4:-81532305 None:intergenic
CGAGACGGCCTGCAAGTGGT+GGG 0.611766 3.4:-81532427 None:intergenic
ATTTGGTGACTTGATTGTAT+GGG 0.612690 3.4:-81532255 None:intergenic
ATGGGATGTTGAATACCACT+TGG 0.613611 3.4:-81532812 None:intergenic
GTGGGTGAAATTGTTTGGAA+CGG 0.621788 3.4:-81533027 None:intergenic
CAAGTGACTGAGTTAGTTGA+CGG 0.622653 3.4:+81532653 MS.gene013787:CDS
GAGGTTATTCCATTTCCCAA+AGG 0.622885 3.4:+81532904 MS.gene013787:CDS
TTTGATTTGGCACTAGAGGA+TGG 0.624229 3.4:-81532361 None:intergenic
ATAGTGAAAGGAAATCGTAT+GGG 0.628856 3.4:-81532830 None:intergenic
ACTTGATTGTATGGGTAGAG+AGG 0.629120 3.4:-81532247 None:intergenic
GTCACTTGCACAGCTAACAA+TGG 0.633273 3.4:-81532638 None:intergenic
TCATGGGCTAAAATCTCGCG+TGG 0.637561 3.4:+81532743 MS.gene013787:CDS
AAACCTGTGGCAATTCGCAG+TGG 0.640198 3.4:+81533376 MS.gene013787:CDS
TTGTTGACGGAGAGTCCACA+TGG 0.640973 3.4:+81532708 MS.gene013787:CDS
GCAGCGGAGAACAATTGCGT+AGG 0.652446 3.4:+81532527 MS.gene013787:CDS
TTACCATAAACATCAAACCA+CGG 0.662908 3.4:-81533337 None:intergenic
ATTGCCTCCAAAATCTTGTG+AGG 0.672285 3.4:-81533472 None:intergenic
ATCGGAGTTCAAAACTACGA+AGG 0.674883 3.4:+81532605 MS.gene013787:CDS
CTAGAACGTTGGTTTCCAAG+TGG 0.685713 3.4:+81532797 MS.gene013787:CDS
TACGCAATTGTTCTCCGCTG+CGG 0.692389 3.4:-81532525 None:intergenic
TGAGTTAATGAAAAGCCATG+TGG 0.717706 3.4:-81532723 None:intergenic
GCTCTTTGTCCATGCCGCAG+CGG 0.721004 3.4:+81532511 MS.gene013787:CDS
ACCATAAACATCAAACCACG+GGG 0.743607 3.4:-81533335 None:intergenic
TAGCCAATAGCAATTCCCCG+TGG 0.764549 3.4:+81533320 MS.gene013787:CDS
ACTCTCCGTCAACAACCGTG+TGG 0.766066 3.4:-81532700 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTGATCAATTGAATT+TGG - chr3.4:81532275-81532294 None:intergenic 15.0%
!! ATTGGCTAATGTATTATTAT+TGG - chr3.4:81533308-81533327 None:intergenic 20.0%
!! CTAAAAAATCTAACAATGTT+TGG - chr3.4:81533280-81533299 None:intergenic 20.0%
!!! AAAAAATTGATTTAACTCCA+TGG + chr3.4:81532288-81532307 MS.gene013787:CDS 20.0%
!!! AAAAATTGATTTAACTCCAT+GGG + chr3.4:81532289-81532308 MS.gene013787:CDS 20.0%
!!! GATTCATTTTTTCCATTAAT+CGG + chr3.4:81532587-81532606 MS.gene013787:CDS 20.0%
! ACACGAAGAAGTTTATTATA+TGG + chr3.4:81533048-81533067 MS.gene013787:CDS 25.0%
! ATATAATAAACTTCTTCGTG+TGG - chr3.4:81533049-81533068 None:intergenic 25.0%
! TATAATAAACTTCTTCGTGT+GGG - chr3.4:81533048-81533067 None:intergenic 25.0%
! TTCAGATGTTGAATTTGATT+TGG - chr3.4:81532377-81532396 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr3.4:81533346-81533365 MS.gene013787:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG + chr3.4:81533351-81533370 MS.gene013787:CDS 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG + chr3.4:81533350-81533369 MS.gene013787:CDS 25.0%
AAAAATTCAATCCATCAGAG+AGG + chr3.4:81532885-81532904 MS.gene013787:CDS 30.0%
AAATGCTGTTGAAGTTTGTA+GGG + chr3.4:81533126-81533145 MS.gene013787:CDS 30.0%
ATAGTGAAAGGAAATCGTAT+GGG - chr3.4:81532833-81532852 None:intergenic 30.0%
GCATTTCCAAAATAATCTTC+AGG - chr3.4:81533112-81533131 None:intergenic 30.0%
TATAGTGAAAGGAAATCGTA+TGG - chr3.4:81532834-81532853 None:intergenic 30.0%
TTACCATAAACATCAAACCA+CGG - chr3.4:81533340-81533359 None:intergenic 30.0%
! AATTTGGTGACTTGATTGTA+TGG - chr3.4:81532259-81532278 None:intergenic 30.0%
! ATGGCTTTTCATTAACTCAT+GGG + chr3.4:81532727-81532746 MS.gene013787:CDS 30.0%
! ATTTGGTGACTTGATTGTAT+GGG - chr3.4:81532258-81532277 None:intergenic 30.0%
!! TATGGTAATGATTTTGGATG+GGG + chr3.4:81533352-81533371 MS.gene013787:CDS 30.0%
!! TGGTTTTACATTTAACCACA+CGG + chr3.4:81532685-81532704 MS.gene013787:CDS 30.0%
!!! TAGTTTTGAACTCCGATTAA+TGG - chr3.4:81532602-81532621 None:intergenic 30.0%
AAATAATCTTCAGGCAATGG+TGG - chr3.4:81533103-81533122 None:intergenic 35.0%
AAATGGAATAACCTCTCTGA+TGG - chr3.4:81532899-81532918 None:intergenic 35.0%
ACTGAAATTGAAAGCCAACA+TGG + chr3.4:81532940-81532959 MS.gene013787:CDS 35.0%
CCAAAATAATCTTCAGGCAA+TGG - chr3.4:81533106-81533125 None:intergenic 35.0%
CGCAGTGGAAATAAAATAAG+TGG + chr3.4:81533391-81533410 MS.gene013787:CDS 35.0%
GAAATGCTGTTGAAGTTTGT+AGG + chr3.4:81533125-81533144 MS.gene013787:CDS 35.0%
GGAAAAAATGAATCGACGAT+GGG - chr3.4:81532581-81532600 None:intergenic 35.0%
GGAATGATTACTGTATTTGC+TGG + chr3.4:81533412-81533431 MS.gene013787:CDS 35.0%
GTTTATTATATGGTCCCGAT+AGG + chr3.4:81533058-81533077 MS.gene013787:CDS 35.0%
TACCATAAACATCAAACCAC+GGG - chr3.4:81533339-81533358 None:intergenic 35.0%
TGAGTTAATGAAAAGCCATG+TGG - chr3.4:81532726-81532745 None:intergenic 35.0%
TGGAAAAAATGAATCGACGA+TGG - chr3.4:81532582-81532601 None:intergenic 35.0%
TGGAAAAGAAGAAGGTAGTA+TGG + chr3.4:81533432-81533451 MS.gene013787:CDS 35.0%
! ATAAAGAAGACCCTTTTTGG+TGG - chr3.4:81532339-81532358 None:intergenic 35.0%
! CATGGCTTTTCATTAACTCA+TGG + chr3.4:81532726-81532745 MS.gene013787:CDS 35.0%
! CCATTGCCTGAAGATTATTT+TGG + chr3.4:81533103-81533122 MS.gene013787:CDS 35.0%
! GTATTTGCTGGAAAAGAAGA+AGG + chr3.4:81533424-81533443 MS.gene013787:CDS 35.0%
! GTGATAAAGAAGACCCTTTT+TGG - chr3.4:81532342-81532361 None:intergenic 35.0%
! TCATCAGAGTCATTCTTTTG+TGG - chr3.4:81532860-81532879 None:intergenic 35.0%
! TTGGAGGGATGATATTTTGT+TGG - chr3.4:81532975-81532994 None:intergenic 35.0%
!! AAGCACTTTTTACACACCTA+TGG + chr3.4:81532993-81533012 MS.gene013787:CDS 35.0%
!! ATAGGTGTGTAAAAAGTGCT+TGG - chr3.4:81532994-81533013 None:intergenic 35.0%
!! TAAAGAAGACCCTTTTTGGT+GGG - chr3.4:81532338-81532357 None:intergenic 35.0%
!! TGAATTTGATTTGGCACTAG+AGG - chr3.4:81532368-81532387 None:intergenic 35.0%
!! TGTGATCTGTTATTTCGAGA+CGG - chr3.4:81532445-81532464 None:intergenic 35.0%
!! TTTTGTGGCTCTATAGTGAA+AGG - chr3.4:81532845-81532864 None:intergenic 35.0%
AAAAATGAATCGACGATGGG+AGG - chr3.4:81532578-81532597 None:intergenic 40.0%
AACTCATAACGCTAGAACGT+TGG + chr3.4:81532786-81532805 MS.gene013787:CDS 40.0%
AATCACTACGAGTCTTGGTT+GGG + chr3.4:81533250-81533269 MS.gene013787:CDS 40.0%
AATGAAAGCAGGACAGTTGT+TGG + chr3.4:81533153-81533172 MS.gene013787:CDS 40.0%
AATTGCGTAGGTGACATACT+TGG + chr3.4:81532539-81532558 MS.gene013787:CDS 40.0%
ACACAACATCCATGAAGTCA+TGG - chr3.4:81533507-81533526 None:intergenic 40.0%
ACCATAAACATCAAACCACG+GGG - chr3.4:81533338-81533357 None:intergenic 40.0%
ACGTTCTAGCGTTATGAGTT+TGG - chr3.4:81532786-81532805 None:intergenic 40.0%
ACTAATTGCACCCACCAAAA+AGG + chr3.4:81532325-81532344 MS.gene013787:CDS 40.0%
ACTTGATTGTATGGGTAGAG+AGG - chr3.4:81532250-81532269 None:intergenic 40.0%
ATCGGAGTTCAAAACTACGA+AGG + chr3.4:81532605-81532624 MS.gene013787:CDS 40.0%
ATGGGATGTTGAATACCACT+TGG - chr3.4:81532815-81532834 None:intergenic 40.0%
ATTGCCTCCAAAATCTTGTG+AGG - chr3.4:81533475-81533494 None:intergenic 40.0%
ATTTAACTCCATGGGATCTC+CGG + chr3.4:81532297-81532316 MS.gene013787:CDS 40.0%
CAAGTGACTGAGTTAGTTGA+CGG + chr3.4:81532653-81532672 MS.gene013787:CDS 40.0%
CTAATTGCACCCACCAAAAA+GGG + chr3.4:81532326-81532345 MS.gene013787:CDS 40.0%
GAGGTTATTCCATTTCCCAA+AGG + chr3.4:81532904-81532923 MS.gene013787:CDS 40.0%
GTGGGTGAAATTGTTTGGAA+CGG - chr3.4:81533030-81533049 None:intergenic 40.0%
TGAAGAATCACTACGAGTCT+TGG + chr3.4:81533245-81533264 MS.gene013787:CDS 40.0%
TGTAGGGTTACAATGAAAGC+AGG + chr3.4:81533142-81533161 MS.gene013787:CDS 40.0%
TTAGTTGACGGCATCTTCAT+TGG + chr3.4:81532665-81532684 MS.gene013787:CDS 40.0%
TTCGTGTGGGTGAAATTGTT+TGG - chr3.4:81533035-81533054 None:intergenic 40.0%
TTGAAAGCCAACATGGAGAT+TGG + chr3.4:81532947-81532966 MS.gene013787:CDS 40.0%
TTTAACCACACGGTTGTTGA+CGG + chr3.4:81532695-81532714 MS.gene013787:CDS 40.0%
! AACCTTGATCTAGCTCCTAT+CGG - chr3.4:81533076-81533095 None:intergenic 40.0%
! AGTTGAGCGATTTTCTCCTT+TGG - chr3.4:81532923-81532942 None:intergenic 40.0%
! GTGTCTTCCTCACAAGATTT+TGG + chr3.4:81533465-81533484 MS.gene013787:CDS 40.0%
! GTTGAGCGATTTTCTCCTTT+GGG - chr3.4:81532922-81532941 None:intergenic 40.0%
! TCTTCCTCACAAGATTTTGG+AGG + chr3.4:81533468-81533487 MS.gene013787:CDS 40.0%
!! CACAAGATTTTGGAGGCAAT+GGG + chr3.4:81533475-81533494 MS.gene013787:CDS 40.0%
!! CGATTTTCTCCTTTGGGAAA+TGG - chr3.4:81532916-81532935 None:intergenic 40.0%
!! GTGTGTAAAAAGTGCTTGGA+GGG - chr3.4:81532990-81533009 None:intergenic 40.0%
!! TCACAAGATTTTGGAGGCAA+TGG + chr3.4:81533474-81533493 MS.gene013787:CDS 40.0%
!! TTTGATTTGGCACTAGAGGA+TGG - chr3.4:81532364-81532383 None:intergenic 40.0%
!! TTTTGGTGGGTGCAATTAGT+AGG - chr3.4:81532325-81532344 None:intergenic 40.0%
AAACCACGGGGAATTGCTAT+TGG - chr3.4:81533326-81533345 None:intergenic 45.0%
ACCAGCATTGTTGCATGTGA+CGG - chr3.4:81532489-81532508 None:intergenic 45.0%
GAATCACTACGAGTCTTGGT+TGG + chr3.4:81533249-81533268 MS.gene013787:CDS 45.0%
GACAGTTGTTGGAAGATGGT+GGG + chr3.4:81533164-81533183 MS.gene013787:CDS 45.0%
GGGAAATGACCATGACTTCA+TGG + chr3.4:81533495-81533514 MS.gene013787:CDS 45.0%
GTCACTTGCACAGCTAACAA+TGG - chr3.4:81532641-81532660 None:intergenic 45.0%
GTTGGTACCAATCTCCATGT+TGG - chr3.4:81532957-81532976 None:intergenic 45.0%
TCCCCGTGGTTTGATGTTTA+TGG + chr3.4:81533334-81533353 MS.gene013787:CDS 45.0%
TGGGTGCAATTAGTAGGTAC+CGG - chr3.4:81532319-81532338 None:intergenic 45.0%
TTGTATGGGTAGAGAGGACT+TGG - chr3.4:81532244-81532263 None:intergenic 45.0%
! AAGTGGTGGGAAAAAGGCAA+GGG - chr3.4:81532417-81532436 None:intergenic 45.0%
! ACCTTGATCTAGCTCCTATC+GGG - chr3.4:81533075-81533094 None:intergenic 45.0%
! GCATGTGACGGAACATGAAA+TGG - chr3.4:81532477-81532496 None:intergenic 45.0%
! TTTTGGATGGGGAAAACCTG+TGG + chr3.4:81533363-81533382 MS.gene013787:CDS 45.0%
!! CTAGAACGTTGGTTTCCAAG+TGG + chr3.4:81532797-81532816 MS.gene013787:CDS 45.0%
!! GGTGTGTAAAAAGTGCTTGG+AGG - chr3.4:81532991-81533010 None:intergenic 45.0%
AAACCTGTGGCAATTCGCAG+TGG + chr3.4:81533376-81533395 MS.gene013787:CDS 50.0%
GCAGGACAGTTGTTGGAAGA+TGG + chr3.4:81533160-81533179 MS.gene013787:CDS 50.0%
GGACAGTTGTTGGAAGATGG+TGG + chr3.4:81533163-81533182 MS.gene013787:CDS 50.0%
TACGCAATTGTTCTCCGCTG+CGG - chr3.4:81532528-81532547 None:intergenic 50.0%
TAGCCAATAGCAATTCCCCG+TGG + chr3.4:81533320-81533339 MS.gene013787:CDS 50.0%
TCCCGATAGGAGCTAGATCA+AGG + chr3.4:81533071-81533090 MS.gene013787:CDS 50.0%
TCCGTCACATGCAACAATGC+TGG + chr3.4:81532485-81532504 MS.gene013787:CDS 50.0%
TTGTTGACGGAGAGTCCACA+TGG + chr3.4:81532708-81532727 MS.gene013787:CDS 50.0%
TTTCCACTGCGAATTGCCAC+AGG - chr3.4:81533382-81533401 None:intergenic 50.0%
! CAAGTGGTGGGAAAAAGGCA+AGG - chr3.4:81532418-81532437 None:intergenic 50.0%
! TCATGGGCTAAAATCTCGCG+TGG + chr3.4:81532743-81532762 MS.gene013787:CDS 50.0%
!! GAAGATGGTGGGCTTGGTAA+AGG + chr3.4:81533175-81533194 MS.gene013787:CDS 50.0%
!! TGGGCTTGGTAAAGGTGCTT+GGG + chr3.4:81533183-81533202 MS.gene013787:CDS 50.0%
!! TTGTTGGAAGATGGTGGGCT+TGG + chr3.4:81533169-81533188 MS.gene013787:CDS 50.0%
AATTGTTCTCCGCTGCGGCA+TGG - chr3.4:81532523-81532542 None:intergenic 55.0%
ACTCTCCGTCAACAACCGTG+TGG - chr3.4:81532703-81532722 None:intergenic 55.0%
ATCGACGATGGGAGGAACGT+AGG - chr3.4:81532570-81532589 None:intergenic 55.0%
ATTTCGAGACGGCCTGCAAG+TGG - chr3.4:81532434-81532453 None:intergenic 55.0%
GCAGCGGAGAACAATTGCGT+AGG + chr3.4:81532527-81532546 MS.gene013787:CDS 55.0%
! AACGGATCACAGAGCGCCAT+AGG - chr3.4:81533012-81533031 None:intergenic 55.0%
! GCCTGCAAGTGGTGGGAAAA+AGG - chr3.4:81532423-81532442 None:intergenic 55.0%
! GCCTTTTTCCCACCACTTGC+AGG + chr3.4:81532419-81532438 MS.gene013787:CDS 55.0%
!! AGTAGGTACCGGAGATCCCA+TGG - chr3.4:81532308-81532327 None:intergenic 55.0%
!! GTGGGCTTGGTAAAGGTGCT+TGG + chr3.4:81533182-81533201 MS.gene013787:CDS 55.0%
GCTCTTTGTCCATGCCGCAG+CGG + chr3.4:81532511-81532530 MS.gene013787:CDS 60.0%
CGAGACGGCCTGCAAGTGGT+GGG - chr3.4:81532430-81532449 None:intergenic 65.0%
TCGAGACGGCCTGCAAGTGG+TGG - chr3.4:81532431-81532450 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 81532230 81533534 81532230 ID=MS.gene013787
chr3.4 mRNA 81532230 81533534 81532230 ID=MS.gene013787.t1;Parent=MS.gene013787
chr3.4 exon 81532230 81533534 81532230 ID=MS.gene013787.t1.exon1;Parent=MS.gene013787.t1
chr3.4 CDS 81532230 81533534 81532230 ID=cds.MS.gene013787.t1;Parent=MS.gene013787.t1
Gene Sequence

>MS.gene013787

ATGGCTGCAATCCAAGTCCTCTCTACCCATACAATCAAGTCACCAAATTCAATTGATCAAAAAATTGATTTAACTCCATGGGATCTCCGGTACCTACTAATTGCACCCACCAAAAAGGGTCTTCTTTATCACCATCCTCTAGTGCCAAATCAAATTCAACATCTGAAACACTCTCTATCTTCTGCCCTTGCCTTTTTCCCACCACTTGCAGGCCGTCTCGAAATAACAGATCACAAAGACAACACCATTTCATGTTCCGTCACATGCAACAATGCTGGTGCGCTCTTTGTCCATGCCGCAGCGGAGAACAATTGCGTAGGTGACATACTTGGATGCACCTACGTTCCTCCCATCGTCGATTCATTTTTTCCATTAATCGGAGTTCAAAACTACGAAGGCACGTCACAGCCATTGTTAGCTGTGCAAGTGACTGAGTTAGTTGACGGCATCTTCATTGGTTTTACATTTAACCACACGGTTGTTGACGGAGAGTCCACATGGCTTTTCATTAACTCATGGGCTAAAATCTCGCGTGGTTGTTGCAATCAAGTATCCAAACTCATAACGCTAGAACGTTGGTTTCCAAGTGGTATTCAACATCCCATACGATTTCCTTTCACTATAGAGCCACAAAAGAATGACTCTGATGATAACGAAAAATTCAATCCATCAGAGAGGTTATTCCATTTCCCAAAGGAGAAAATCGCTCAACTGAAATTGAAAGCCAACATGGAGATTGGTACCAACAAAATATCATCCCTCCAAGCACTTTTTACACACCTATGGCGCTCTGTGATCCGTTCCAAACAATTTCACCCACACGAAGAAGTTTATTATATGGTCCCGATAGGAGCTAGATCAAGGTTTGTTCCACCATTGCCTGAAGATTATTTTGGAAATGCTGTTGAAGTTTGTAGGGTTACAATGAAAGCAGGACAGTTGTTGGAAGATGGTGGGCTTGGTAAAGGTGCTTGGGCGATACACAAAATGATTTCTTTGCAGTCAAATGAGAAGTTGAAGAATCACTACGAGTCTTGGTTGGGAAATCCAAACATTGTTAGATTTTTTAGTGCGACCAATAATAATACATTAGCCAATAGCAATTCCCCGTGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAACCTGTGGCAATTCGCAGTGGAAATAAAATAAGTGGAATGATTACTGTATTTGCTGGAAAAGAAGAAGGTAGTATGGACTTTCAAGTGTGTCTTCCTCACAAGATTTTGGAGGCAATGGGAAATGACCATGACTTCATGGATGTTGTGTCTATTTAA
Protein sequence

>MS.gene013787.t1

MAAIQVLSTHTIKSPNSIDQKIDLTPWDLRYLLIAPTKKGLLYHHPLVPNQIQHLKHSLSSALAFFPPLAGRLEITDHKDNTISCSVTCNNAGALFVHAAAENNCVGDILGCTYVPPIVDSFFPLIGVQNYEGTSQPLLAVQVTELVDGIFIGFTFNHTVVDGESTWLFINSWAKISRGCCNQVSKLITLERWFPSGIQHPIRFPFTIEPQKNDSDDNEKFNPSERLFHFPKEKIAQLKLKANMEIGTNKISSLQALFTHLWRSVIRSKQFHPHEEVYYMVPIGARSRFVPPLPEDYFGNAVEVCRVTMKAGQLLEDGGLGKGAWAIHKMISLQSNEKLKNHYESWLGNPNIVRFFSATNNNTLANSNSPWFDVYGNDFGWGKPVAIRSGNKISGMITVFAGKEEGSMDFQVCLPHKILEAMGNDHDFMDVVSI