AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063868

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063868.t1 MTR_4g094835 70.861 453 122 5 1 450 1 446 0.0 644
MS.gene063868.t1 MTR_4g094838 68.212 453 117 5 4 450 1 432 0.0 599
MS.gene063868.t1 MTR_4g094832 72.606 376 92 3 81 450 60 430 0.0 549
MS.gene063868.t1 MTR_4g094842 59.471 454 166 9 1 446 1 444 0.0 524
MS.gene063868.t1 MTR_0190s0020 57.366 448 179 7 1 446 1 438 0.0 514
MS.gene063868.t1 MTR_8g041770 57.206 451 174 9 1 446 1 437 1.53e-179 509
MS.gene063868.t1 MTR_4g022720 55.111 450 179 9 1 446 1 431 7.23e-168 479
MS.gene063868.t1 MTR_3g088420 54.464 448 183 9 1 446 1 429 1.82e-167 478
MS.gene063868.t1 MTR_4g094828 54.783 460 184 11 1 448 1 448 1.54e-165 474
MS.gene063868.t1 MTR_4g022825 54.305 453 183 9 1 446 1 436 4.21e-165 472
MS.gene063868.t1 MTR_3g026310 52.928 461 185 11 1 446 1 444 3.74e-164 470
MS.gene063868.t1 MTR_4g022800 54.386 456 180 10 1 446 1 438 8.87e-164 469
MS.gene063868.t1 MTR_4g022670 52.747 455 188 9 1 446 1 437 4.69e-162 464
MS.gene063868.t1 MTR_8g079710 56.222 450 174 11 1 441 1 436 1.90e-161 463
MS.gene063868.t1 MTR_7g026860 53.304 454 184 11 1 446 1 434 2.60e-158 455
MS.gene063868.t1 MTR_4g022290 53.947 456 185 10 1 446 1 441 1.34e-156 451
MS.gene063868.t1 MTR_4g022760 48.899 454 163 8 1 446 1 393 1.06e-140 409
MS.gene063868.t1 MTR_6g016815 42.953 447 170 11 1 446 15 377 1.03e-107 324
MS.gene063868.t1 MTR_3g101900 39.394 429 241 9 27 446 26 444 3.72e-87 273
MS.gene063868.t1 MTR_4g094825 80.925 173 31 2 19 191 80 250 7.93e-87 271
MS.gene063868.t1 MTR_4g094825 71.333 150 33 1 301 450 252 391 1.22e-65 216
MS.gene063868.t1 MTR_3g102020 38.362 464 253 11 3 450 5 451 1.04e-84 267
MS.gene063868.t1 MTR_3g101930 38.568 433 238 9 31 446 30 451 1.52e-83 264
MS.gene063868.t1 MTR_3g102010 37.991 458 252 11 4 446 7 447 5.44e-82 260
MS.gene063868.t1 MTR_3g101970 37.004 454 251 10 3 446 5 433 7.90e-81 256
MS.gene063868.t1 MTR_3g101960 37.991 458 252 12 3 445 5 445 1.64e-80 256
MS.gene063868.t1 MTR_8g040760 55.856 222 88 5 1 220 1 214 1.47e-75 236
MS.gene063868.t1 MTR_3g070170 33.115 459 251 7 29 450 31 470 1.81e-73 239
MS.gene063868.t1 MTR_8g040780 55.714 210 88 3 239 445 20 227 1.82e-73 231
MS.gene063868.t1 MTR_5g081960 32.285 477 247 9 29 447 25 483 8.40e-70 229
MS.gene063868.t1 MTR_4g015180 32.394 497 261 9 8 446 4 483 2.06e-67 223
MS.gene063868.t1 MTR_7g026850 55.484 155 63 2 39 193 15 163 3.26e-53 176
MS.gene063868.t1 MTR_7g026770 47.179 195 75 4 154 345 132 301 1.35e-45 160
MS.gene063868.t1 MTR_6g045553 28.696 460 283 12 21 447 25 472 3.70e-45 164
MS.gene063868.t1 MTR_3g102030 34.202 307 161 7 103 403 48 319 1.89e-43 155
MS.gene063868.t1 MTR_6g034485 53.846 130 55 2 199 328 1 125 2.89e-42 146
MS.gene063868.t1 MTR_6g034495 52.857 140 59 2 52 191 28 160 4.14e-42 147
MS.gene063868.t1 MTR_7g026830 63.415 82 30 0 97 178 16 97 4.60e-30 112
MS.gene063868.t1 MTR_3g026950 25.296 423 258 14 48 435 38 437 9.53e-29 118
MS.gene063868.t1 MTR_3g049150 26.442 416 258 13 48 435 38 433 3.18e-28 116
MS.gene063868.t1 MTR_4g007540 28.321 399 239 17 64 441 56 428 3.88e-28 115
MS.gene063868.t1 MTR_8g075610 26.972 393 244 16 64 439 47 413 1.07e-27 114
MS.gene063868.t1 MTR_8g096530 29.915 351 210 14 64 402 59 385 1.61e-26 111
MS.gene063868.t1 MTR_3g098980 29.226 349 212 15 64 401 66 390 2.07e-24 105
MS.gene063868.t1 MTR_2g105330 26.098 387 242 16 64 431 56 417 6.58e-23 100
MS.gene063868.t1 MTR_4g097090 25.792 442 250 18 39 431 28 440 7.57e-23 100
MS.gene063868.t1 MTR_2g064495 26.098 387 242 16 64 431 56 417 1.35e-22 100
MS.gene063868.t1 MTR_4g127650 24.779 452 273 17 26 439 26 448 4.76e-22 98.6
MS.gene063868.t1 MTR_7g026800 38.365 159 65 5 228 384 29 156 1.34e-20 89.7
MS.gene063868.t1 MTR_7g027175 24.675 385 266 11 59 437 63 429 1.68e-20 94.0
MS.gene063868.t1 MTR_3g049140 28.692 237 140 8 216 435 6 230 9.93e-19 85.9
MS.gene063868.t1 MTR_3g071320 25.378 331 199 10 53 359 49 355 1.16e-18 88.2
MS.gene063868.t1 MTR_4g013050 25.121 414 227 18 56 441 46 404 1.24e-18 88.2
MS.gene063868.t1 MTR_7g084940 27.246 345 221 13 64 399 69 392 1.52e-18 88.2
MS.gene063868.t1 MTR_7g026780 43.519 108 35 3 339 446 26 107 6.12e-18 79.7
MS.gene063868.t1 MTR_6g015815 23.341 437 293 12 29 439 32 452 4.65e-17 83.6
MS.gene063868.t1 MTR_1g081660 24.011 354 248 9 51 400 60 396 1.65e-16 82.0
MS.gene063868.t1 MTR_7g085930 24.605 443 256 19 45 435 31 447 3.06e-16 81.3
MS.gene063868.t1 MTR_6g015850 23.160 462 311 13 3 439 7 449 1.13e-15 79.3
MS.gene063868.t1 MTR_5g084850 23.821 424 268 20 38 446 37 420 1.70e-14 75.5
MS.gene063868.t1 MTR_8g079970 22.632 380 243 13 38 400 37 382 2.09e-14 75.5
MS.gene063868.t1 MTR_5g084870 23.544 395 245 18 48 423 41 397 2.13e-14 75.5
MS.gene063868.t1 MTR_4g055170 25.249 301 205 7 64 358 69 355 2.39e-14 75.1
MS.gene063868.t1 MTR_5g026320 23.821 424 263 17 49 445 58 448 2.51e-14 75.1
MS.gene063868.t1 MTR_2g089765 22.614 482 294 20 3 434 15 467 4.16e-14 74.7
MS.gene063868.t1 MTR_7g085990 23.288 438 278 16 45 439 27 449 7.59e-14 73.6
MS.gene063868.t1 MTR_6g015315 22.436 312 212 7 152 440 141 445 8.46e-14 73.6
MS.gene063868.t1 MTR_6g015830 22.698 467 310 14 3 439 7 452 9.92e-14 73.2
MS.gene063868.t1 MTR_8g012750 24.155 414 256 16 29 399 24 422 1.75e-13 72.8
MS.gene063868.t1 MTR_3g112180 24.595 309 212 9 54 353 51 347 2.79e-13 72.0
MS.gene063868.t1 MTR_3g087640 25.056 447 276 18 38 437 28 462 9.14e-13 70.5
MS.gene063868.t1 MTR_7g014330 24.938 405 247 18 31 399 26 409 1.87e-12 69.3
MS.gene063868.t1 MTR_3g028210 24.468 470 284 20 4 431 8 448 2.01e-12 69.3
MS.gene063868.t1 MTR_7g026870 50.000 70 29 2 295 358 82 151 2.05e-12 65.1
MS.gene063868.t1 MTR_6g015320 25.566 309 199 11 152 437 144 444 2.19e-12 69.3
MS.gene063868.t1 MTR_7g086000 26.341 410 252 18 62 439 58 449 2.30e-12 68.9
MS.gene063868.t1 MTR_6g053810 24.367 316 211 12 58 362 65 363 4.13e-12 68.2
MS.gene063868.t1 MTR_1g094790 25.592 422 243 20 58 446 54 437 7.44e-12 67.4
MS.gene063868.t1 MTR_2g015430 24.157 356 238 13 64 408 58 392 8.97e-12 67.0
MS.gene063868.t1 MTR_7g014160 24.198 405 252 15 64 431 69 455 1.01e-11 67.0
MS.gene063868.t1 MTR_7g084910 25.188 266 169 7 64 316 70 318 1.32e-11 66.6
MS.gene063868.t1 MTR_7g015860 22.277 404 259 12 64 434 64 445 4.15e-11 65.1
MS.gene063868.t1 MTR_3g465980 24.662 296 198 12 49 332 49 331 7.18e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063868.t1 AT5G67150 40.312 449 247 9 6 446 7 442 2.40e-103 315
MS.gene063868.t1 AT3G50300 39.121 455 252 12 1 444 1 441 3.98e-94 291
MS.gene063868.t1 AT5G67160 39.623 424 230 8 27 445 25 427 3.46e-92 286
MS.gene063868.t1 AT3G50280 36.283 452 269 9 1 446 1 439 1.06e-89 280
MS.gene063868.t1 AT3G50270 37.528 445 267 7 6 446 7 444 7.47e-89 278
MS.gene063868.t1 AT5G38130 38.952 439 245 10 1 432 21 443 4.29e-88 276
MS.gene063868.t1 AT5G42830 35.927 437 246 11 27 446 26 445 3.53e-76 245
MS.gene063868.t1 AT2G39980 32.623 469 246 7 29 446 27 476 9.09e-76 245
MS.gene063868.t1 AT5G07860 37.500 432 237 10 31 446 31 445 2.40e-74 240
MS.gene063868.t1 AT5G01210 34.430 456 246 9 29 446 25 465 7.62e-70 229
MS.gene063868.t1 AT5G07850 36.279 430 249 10 31 446 31 449 1.76e-69 228
MS.gene063868.t1 AT5G07870 35.455 440 247 9 31 446 31 457 3.48e-67 222
MS.gene063868.t1 AT3G50280 36.859 312 191 3 138 446 1 309 7.92e-65 211
MS.gene063868.t1 AT3G50290 38.806 268 157 3 135 397 4 269 1.85e-55 186
MS.gene063868.t1 AT5G23940 31.881 436 275 12 27 447 21 449 1.56e-54 189
MS.gene063868.t1 AT3G48720 26.357 387 258 12 62 439 59 427 3.79e-24 104
MS.gene063868.t1 AT5G41040 27.746 346 228 10 61 400 66 395 9.45e-24 103
MS.gene063868.t1 AT5G41040 27.746 346 228 10 61 400 82 411 1.22e-23 103
MS.gene063868.t1 AT2G19070 26.058 449 268 20 28 439 22 443 3.23e-23 102
MS.gene063868.t1 AT3G29590 25.688 436 282 13 29 441 26 442 3.95e-23 101
MS.gene063868.t1 AT5G48930 26.329 395 244 15 64 437 56 424 4.14e-22 98.6
MS.gene063868.t1 AT5G63560 26.203 374 240 14 64 427 61 408 4.11e-20 92.8
MS.gene063868.t1 AT2G40230 24.755 408 278 9 1 400 1 387 1.58e-18 87.8
MS.gene063868.t1 AT1G28680 25.434 346 229 11 64 400 62 387 2.61e-17 84.3
MS.gene063868.t1 AT3G29670 23.632 457 296 16 3 431 2 433 2.10e-16 81.6
MS.gene063868.t1 AT3G30280 27.541 305 176 15 25 316 23 295 2.99e-16 81.3
MS.gene063868.t1 AT1G28680 25.000 300 203 8 64 356 62 346 5.47e-16 80.1
MS.gene063868.t1 AT5G57840 24.221 417 250 18 48 431 39 422 1.35e-15 79.0
MS.gene063868.t1 AT1G24420 24.031 387 252 14 74 450 75 429 1.94e-15 78.6
MS.gene063868.t1 AT1G24420 24.031 387 252 14 74 450 75 429 1.94e-15 78.6
MS.gene063868.t1 AT5G07080 25.831 391 234 19 37 400 42 403 2.71e-15 78.2
MS.gene063868.t1 AT3G29635 24.594 431 278 15 29 431 27 438 4.45e-15 77.4
MS.gene063868.t1 AT1G24430 23.514 370 236 14 44 400 41 376 1.62e-14 75.9
MS.gene063868.t1 AT5G17540 24.552 391 248 13 32 400 23 388 2.12e-14 75.5
MS.gene063868.t1 AT3G03480 24.740 384 231 12 48 409 56 403 1.03e-13 73.2
MS.gene063868.t1 AT5G47950 24.771 436 275 16 32 450 20 419 1.05e-13 73.2
MS.gene063868.t1 AT1G31490 29.583 240 135 11 52 282 50 264 1.09e-13 73.2
MS.gene063868.t1 AT3G47170 25.088 283 188 10 64 330 70 344 1.53e-13 72.8
MS.gene063868.t1 AT1G32910 23.743 358 229 10 64 400 65 399 5.24e-13 71.2
MS.gene063868.t1 AT5G23970 23.153 406 263 16 58 447 46 418 6.34e-13 70.9
MS.gene063868.t1 AT2G23510 24.217 351 231 11 64 400 69 398 1.82e-12 69.3
MS.gene063868.t1 AT5G61160 23.179 302 210 9 152 437 143 438 4.25e-12 68.2
MS.gene063868.t1 AT1G65450 21.944 360 240 13 57 399 65 400 5.05e-12 68.2
MS.gene063868.t1 AT2G25150 22.376 362 250 11 56 403 59 403 2.50e-11 65.9
MS.gene063868.t1 AT5G16410 22.622 389 256 12 29 400 59 419 2.51e-11 65.9

Find 109 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCTTTCTTATGAGATTT+TGG 0.232782 8.1:-62193766 MS.gene063868:CDS
GAGGTGTGTAGTTGGATTTC+AGG 0.234977 8.1:+62194687 None:intergenic
TTGCAGACGGCAAGTCATTT+TGG 0.280516 8.1:-62194535 MS.gene063868:CDS
TCATTCTTTCCACCCTTTGC+AGG 0.283726 8.1:-62194836 MS.gene063868:CDS
TTGGTTTGATGGGTTGAGTA+TGG 0.297410 8.1:+62194895 None:intergenic
TACATTCTGATGAGAAAATA+AGG 0.300914 8.1:-62194010 MS.gene063868:CDS
GGAAACGCAGCACTGATTAA+TGG 0.307186 8.1:-62194110 MS.gene063868:CDS
TTTGGCATTTCGTTAATTCT+TGG 0.313993 8.1:-62194517 MS.gene063868:CDS
AACCAACATTACTTAAGAAC+TGG 0.316331 8.1:+62193710 None:intergenic
AGATGGTTGATTTGGTTTGA+TGG 0.316804 8.1:+62194884 None:intergenic
TAAGAATCGTAGGAGAATTA+TGG 0.337387 8.1:-62193829 MS.gene063868:CDS
TGATGTGTTGTTGTTGTTTG+TGG 0.353296 8.1:+62194784 None:intergenic
CCACTTGAAACCATGCAAAT+AGG 0.358534 8.1:-62194935 MS.gene063868:CDS
CCGCTAGAGGAGGATTATTT+CGG 0.364413 8.1:-62194131 MS.gene063868:CDS
TTTGTTTGAGATGGTTGATT+TGG 0.369144 8.1:+62194876 None:intergenic
GTCATGATTGTTGTTGTCAA+TGG 0.377227 8.1:+62195009 None:intergenic
ATTGGAATTTCAATATCGTT+TGG 0.377241 8.1:+62194434 None:intergenic
TTGGAATTTCAATATCGTTT+GGG 0.377876 8.1:+62194435 None:intergenic
GATGGTTGATTTGGTTTGAT+GGG 0.387524 8.1:+62194885 None:intergenic
TGTGAAATGGAAGATTCTTT+CGG 0.389981 8.1:+62194340 None:intergenic
GAAGATTCTTTCGGCTAACT+TGG 0.393128 8.1:+62194349 None:intergenic
AAGCGTTATTATCTCACGTT+TGG 0.394991 8.1:-62194241 MS.gene063868:CDS
GGGAAATGATGCTGAGTTCA+TGG 0.395255 8.1:-62193736 MS.gene063868:CDS
TATTTCCATGTGTTGAGTAT+AGG 0.408690 8.1:-62194176 MS.gene063868:CDS
ATGGGTTGAGTATGGGTTTG+TGG 0.421764 8.1:+62194903 None:intergenic
TGGTTTGATGGGTTGAGTAT+GGG 0.425740 8.1:+62194896 None:intergenic
TTAAGTAACGGCGTCTTCAT+TGG 0.427513 8.1:-62194578 MS.gene063868:CDS
TTTATGAAAGGAAAGCGTAT+TGG 0.432580 8.1:+62194416 None:intergenic
TGGACTTGGAAAAGTTGCTT+TGG 0.433532 8.1:-62194054 MS.gene063868:CDS
GTTTGTGGTGTCATTGTGTT+GGG 0.435350 8.1:+62194799 None:intergenic
AGTGGTAATTTGTTAGCAAC+CGG 0.438639 8.1:-62193930 MS.gene063868:CDS
TAAGGAACCATTACGAGTCT+TGG 0.447357 8.1:-62193992 MS.gene063868:CDS
GAGGTTCTAGAGTGTGGACT+TGG 0.456876 8.1:-62194068 MS.gene063868:CDS
TGTTGTTGTCAATGGGTGCT+TGG 0.462813 8.1:+62195017 None:intergenic
GGATTTCAGGATGTCGTTAA+CGG 0.463253 8.1:+62194700 None:intergenic
TCAAAAGCAAATTTAGAAGC+TGG 0.463731 8.1:-62194293 MS.gene063868:CDS
TGTTTGTGGTGTCATTGTGT+TGG 0.465080 8.1:+62194798 None:intergenic
AGCGTTGTTCTGAGGAGCCT+TGG 0.465886 8.1:+62194378 None:intergenic
TCATGATTGTTGTTGTCAAT+GGG 0.469209 8.1:+62195010 None:intergenic
TCTTGGTCCCAAATCTCAAA+AGG 0.471773 8.1:-62194500 MS.gene063868:CDS
GGGAACCAACGTTGAAATGA+AGG 0.474068 8.1:+62194455 None:intergenic
CGTTACTTAACTCCGTTACC+TGG 0.475793 8.1:+62194591 None:intergenic
TACAATTGGAGGTGTGTAGT+TGG 0.480235 8.1:+62194679 None:intergenic
TGGAAAGGGTTTGTTTGAGA+TGG 0.483593 8.1:+62194867 None:intergenic
CATTCTTTCCACCCTTTGCA+GGG 0.499452 8.1:-62194835 MS.gene063868:CDS
TTCTCAACCAAGACTCGTAA+TGG 0.508077 8.1:+62193985 None:intergenic
AATGGTGTGAGGATGAAAGC+AGG 0.512088 8.1:-62194092 MS.gene063868:CDS
GAATGATAGAGTAGTGGAAA+GGG 0.514584 8.1:+62194853 None:intergenic
GTATGGGTTTGTGGAAAAGA+AGG 0.518697 8.1:+62194912 None:intergenic
CCACTTGGAGAACTACCACT+TGG 0.529940 8.1:+62193948 None:intergenic
ACCAACATTACTTAAGAACT+GGG 0.537374 8.1:+62193711 None:intergenic
AAGCCTGTGGCATTTCGACG+TGG 0.538100 8.1:-62193861 MS.gene063868:CDS
AAAACCAAGTCTAGGAGTCC+AGG 0.540823 8.1:-62194609 MS.gene063868:CDS
ACCGAGGTTGTTGCAAATGA+TGG 0.541381 8.1:+62194757 None:intergenic
ATGGTGTGAGGATGAAAGCA+GGG 0.541587 8.1:-62194091 MS.gene063868:CDS
AGCCTGTGGCATTTCGACGT+GGG 0.542075 8.1:-62193860 MS.gene063868:CDS
GATTACAAGACGCCCTGCAA+AGG 0.543778 8.1:+62194823 None:intergenic
TTTGCATGGTTTCAAGTGGA+AGG 0.545068 8.1:+62194939 None:intergenic
ATGGTTTCAAGTGGAAGGTA+TGG 0.545878 8.1:+62194944 None:intergenic
TAGTGGTGGAGATCACTTTG+AGG 0.547449 8.1:+62195044 None:intergenic
GAAAAGAAGGCCTATTTGCA+TGG 0.551002 8.1:+62194925 None:intergenic
AGTGGTGGAGATCACTTTGA+GGG 0.551067 8.1:+62195045 None:intergenic
GAAAATAGAATTAACACCAT+GGG 0.553345 8.1:-62194972 MS.gene063868:CDS
AACTCCCTTCATTTCAACGT+TGG 0.554301 8.1:-62194460 MS.gene063868:CDS
ATTACAAGACGCCCTGCAAA+GGG 0.555357 8.1:+62194824 None:intergenic
AGAATGATAGAGTAGTGGAA+AGG 0.559863 8.1:+62194852 None:intergenic
TGCAACCACGTTGTTGCAGA+CGG 0.561465 8.1:-62194548 MS.gene063868:CDS
ACATGGAAATAATCTTCTTG+TGG 0.561680 8.1:+62194188 None:intergenic
GTTACCTGGACTCCTAGACT+TGG 0.573860 8.1:+62194605 None:intergenic
GTGTATGCTGGTGCTGAAGA+AGG 0.576122 8.1:-62193807 MS.gene063868:CDS
GGAACCAACGTTGAAATGAA+GGG 0.576282 8.1:+62194456 None:intergenic
GGATTCTAAGCGTTGTTCTG+AGG 0.579044 8.1:+62194370 None:intergenic
GCTACGTCAAAACCAAGTCT+AGG 0.579161 8.1:-62194617 MS.gene063868:CDS
CCTATTTGCATGGTTTCAAG+TGG 0.580273 8.1:+62194935 None:intergenic
AACAAACCACAGAGAACCGC+CGG 0.580605 8.1:+62193911 None:intergenic
CTACCACTTGGAAACAACCT+TGG 0.580849 8.1:+62193960 None:intergenic
GCCTGTGGCATTTCGACGTG+GGG 0.582822 8.1:-62193859 MS.gene063868:CDS
TCCATCATTTGCAACAACCT+CGG 0.586818 8.1:-62194758 MS.gene063868:CDS
GGGGTACTAATAAGAATCGT+AGG 0.588500 8.1:-62193839 MS.gene063868:CDS
GCAGGGGAGGTTCTAGAGTG+TGG 0.591414 8.1:-62194074 MS.gene063868:CDS
TGGAAAGAATGATAGAGTAG+TGG 0.593438 8.1:+62194847 None:intergenic
AGGAGAATTATGGTGTATGC+TGG 0.594082 8.1:-62193819 MS.gene063868:CDS
GGAAGGTATGGAAGATCCCA+TGG 0.605846 8.1:+62194956 None:intergenic
AAATAATCCTCCTCTAGCGG+TGG 0.608587 8.1:+62194134 None:intergenic
ACGTATGATTCCACCGCTAG+AGG 0.610874 8.1:-62194144 MS.gene063868:CDS
TAGCAACCGGCGGTTCTCTG+TGG 0.611305 8.1:-62193917 MS.gene063868:CDS
AGAAAATAGAATTAACACCA+TGG 0.614555 8.1:-62194973 MS.gene063868:CDS
AAGTCTAGGAGTCCAGGTAA+CGG 0.615804 8.1:-62194603 MS.gene063868:CDS
GGGTGCTTGGATTGTAGTGG+TGG 0.619683 8.1:+62195030 None:intergenic
CCGAAATAATCCTCCTCTAG+CGG 0.625415 8.1:+62194131 None:intergenic
ACAAGACGCCCTGCAAAGGG+TGG 0.634893 8.1:+62194827 None:intergenic
TAACACCTATACTCAACACA+TGG 0.635510 8.1:+62194171 None:intergenic
AAGAATCTTCCATTTCACAA+AGG 0.638577 8.1:-62194336 MS.gene063868:CDS
AATGGGTGCTTGGATTGTAG+TGG 0.638880 8.1:+62195027 None:intergenic
TATGATTCCACCGCTAGAGG+AGG 0.641886 8.1:-62194141 MS.gene063868:CDS
GGTAATTTGTTAGCAACCGG+CGG 0.644041 8.1:-62193927 MS.gene063868:CDS
GTTGCTAACAAATTACCACT+TGG 0.646151 8.1:+62193933 None:intergenic
TGGTGTGAGGATGAAAGCAG+GGG 0.647651 8.1:-62194090 MS.gene063868:CDS
CAGGTAACGGAGTTAAGTAA+CGG 0.651196 8.1:-62194590 MS.gene063868:CDS
TGTGAGGATGAAAGCAGGGG+AGG 0.662636 8.1:-62194087 MS.gene063868:CDS
ACACCAAGGTTGTTTCCAAG+TGG 0.665409 8.1:-62193963 MS.gene063868:CDS
AAAACAGAATCACAAAACCA+AGG 0.673515 8.1:-62194395 MS.gene063868:CDS
CCAAGTGGTAGTTCTCCAAG+TGG 0.673628 8.1:-62193948 MS.gene063868:CDS
CAGCACTGATTAATGGTGTG+AGG 0.700246 8.1:-62194103 MS.gene063868:CDS
CCTGTGGCATTTCGACGTGG+GGG 0.706268 8.1:-62193858 MS.gene063868:CDS
TCTAGCGGTGGAATCATACG+TGG 0.721117 8.1:+62194146 None:intergenic
AGTCTTGGTTGAGAACACCA+AGG 0.770075 8.1:-62193977 MS.gene063868:CDS
ACTTGCCGTCTGCAACAACG+TGG 0.788179 8.1:+62194543 None:intergenic
CGTGGACGAAGAGTGCACCG+AGG 0.794757 8.1:+62194741 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTGCTTTTGATTTCAATT+CGG + chr8.1:62194473-62194492 None:intergenic 20.0%
! AGAAAATAGAATTAACACCA+TGG - chr8.1:62193801-62193820 MS.gene063868:CDS 25.0%
! ATTGGAATTTCAATATCGTT+TGG + chr8.1:62194343-62194362 None:intergenic 25.0%
! GAAAATAGAATTAACACCAT+GGG - chr8.1:62193802-62193821 MS.gene063868:CDS 25.0%
! TACATTCTGATGAGAAAATA+AGG - chr8.1:62194764-62194783 MS.gene063868:CDS 25.0%
! TTGGAATTTCAATATCGTTT+GGG + chr8.1:62194342-62194361 None:intergenic 25.0%
!! TATTTTCTCATCAGAATGTA+AGG + chr8.1:62194764-62194783 None:intergenic 25.0%
!! TTGTCTTTCTTATGAGATTT+TGG - chr8.1:62195008-62195027 MS.gene063868:CDS 25.0%
!!! TGTGATTCTGTTTTTATGAA+AGG + chr8.1:62194373-62194392 None:intergenic 25.0%
AAAAATGAGTGTACAATTGG+AGG + chr8.1:62194109-62194128 None:intergenic 30.0%
AAAACAGAATCACAAAACCA+AGG - chr8.1:62194379-62194398 MS.gene063868:CDS 30.0%
AAGAATCTTCCATTTCACAA+AGG - chr8.1:62194438-62194457 MS.gene063868:CDS 30.0%
ACATGGAAATAATCTTCTTG+TGG + chr8.1:62194589-62194608 None:intergenic 30.0%
GGAAAAAATGAGTGTACAAT+TGG + chr8.1:62194112-62194131 None:intergenic 30.0%
TAAGAATCGTAGGAGAATTA+TGG - chr8.1:62194945-62194964 MS.gene063868:CDS 30.0%
TATTTCCATGTGTTGAGTAT+AGG - chr8.1:62194598-62194617 MS.gene063868:CDS 30.0%
TCAAAAGCAAATTTAGAAGC+TGG - chr8.1:62194481-62194500 MS.gene063868:CDS 30.0%
TGTGAAATGGAAGATTCTTT+CGG + chr8.1:62194437-62194456 None:intergenic 30.0%
TTTGGCATTTCGTTAATTCT+TGG - chr8.1:62194257-62194276 MS.gene063868:CDS 30.0%
! CAATTTTCTCCTTTGTGAAA+TGG + chr8.1:62194450-62194469 None:intergenic 30.0%
! TCATGATTGTTGTTGTCAAT+GGG + chr8.1:62193767-62193786 None:intergenic 30.0%
! TCTTTCTTATGAGATTTTGG+AGG - chr8.1:62195011-62195030 MS.gene063868:CDS 30.0%
! TTTATGAAAGGAAAGCGTAT+TGG + chr8.1:62194361-62194380 None:intergenic 30.0%
! TTTGTTTGAGATGGTTGATT+TGG + chr8.1:62193901-62193920 None:intergenic 30.0%
!! TAGTTTTTAACTCCGTTAAG+TGG + chr8.1:62194133-62194152 None:intergenic 30.0%
!! TTTTTGGCAATGATTTTGGT+TGG - chr8.1:62194887-62194906 MS.gene063868:CDS 30.0%
!!! AAGGGAGTTTTGTTATTTTG+GGG + chr8.1:62194303-62194322 None:intergenic 30.0%
!!! CACTCATTTTTTCCACTTAA+CGG - chr8.1:62194118-62194137 MS.gene063868:CDS 30.0%
!!! GAAGGGAGTTTTGTTATTTT+GGG + chr8.1:62194304-62194323 None:intergenic 30.0%
!!! GTTGTTTTTGGCAATGATTT+TGG - chr8.1:62194883-62194902 MS.gene063868:CDS 30.0%
!!! TGAAGGGAGTTTTGTTATTT+TGG + chr8.1:62194305-62194324 None:intergenic 30.0%
!!! TTTTGGCAATGATTTTGGTT+GGG - chr8.1:62194888-62194907 MS.gene063868:CDS 30.0%
AAGCGTTATTATCTCACGTT+TGG - chr8.1:62194533-62194552 MS.gene063868:CDS 35.0%
GTTGCTAACAAATTACCACT+TGG + chr8.1:62194844-62194863 None:intergenic 35.0%
TAACACCTATACTCAACACA+TGG + chr8.1:62194606-62194625 None:intergenic 35.0%
TGGAAAGAATGATAGAGTAG+TGG + chr8.1:62193930-62193949 None:intergenic 35.0%
! AGAATGATAGAGTAGTGGAA+AGG + chr8.1:62193925-62193944 None:intergenic 35.0%
! CTTCTTGTGGATCAAGTTTT+TGG + chr8.1:62194576-62194595 None:intergenic 35.0%
! GAATGATAGAGTAGTGGAAA+GGG + chr8.1:62193924-62193943 None:intergenic 35.0%
! GTCATGATTGTTGTTGTCAA+TGG + chr8.1:62193768-62193787 None:intergenic 35.0%
!! AGATGGTTGATTTGGTTTGA+TGG + chr8.1:62193893-62193912 None:intergenic 35.0%
!! AGTGGTAATTTGTTAGCAAC+CGG - chr8.1:62194844-62194863 MS.gene063868:CDS 35.0%
!! GATGGTTGATTTGGTTTGAT+GGG + chr8.1:62193892-62193911 None:intergenic 35.0%
!! TATGAGATTTTGGAGGCTTT+GGG - chr8.1:62195018-62195037 MS.gene063868:CDS 35.0%
!! TGATGTGTTGTTGTTGTTTG+TGG + chr8.1:62193993-62194012 None:intergenic 35.0%
!! TTATGAGATTTTGGAGGCTT+TGG - chr8.1:62195017-62195036 MS.gene063868:CDS 35.0%
!!! AGGGAGTTTTGTTATTTTGG+GGG + chr8.1:62194302-62194321 None:intergenic 35.0%
!!! TCTCTGTGGTTTGTTGTTTT+TGG - chr8.1:62194871-62194890 MS.gene063868:CDS 35.0%
!!! TTTGGCAATGATTTTGGTTG+GGG - chr8.1:62194889-62194908 MS.gene063868:CDS 35.0%
AACTCCCTTCATTTCAACGT+TGG - chr8.1:62194314-62194333 MS.gene063868:CDS 40.0%
AGGAGAATTATGGTGTATGC+TGG - chr8.1:62194955-62194974 MS.gene063868:CDS 40.0%
CCACTTGAAACCATGCAAAT+AGG - chr8.1:62193839-62193858 MS.gene063868:CDS 40.0%
CCTATTTGCATGGTTTCAAG+TGG + chr8.1:62193842-62193861 None:intergenic 40.0%
GAAAAGAAGGCCTATTTGCA+TGG + chr8.1:62193852-62193871 None:intergenic 40.0%
GAAGATTCTTTCGGCTAACT+TGG + chr8.1:62194428-62194447 None:intergenic 40.0%
GGAACCAACGTTGAAATGAA+GGG + chr8.1:62194321-62194340 None:intergenic 40.0%
GGATTTCAGGATGTCGTTAA+CGG + chr8.1:62194077-62194096 None:intergenic 40.0%
GTATGGGTTTGTGGAAAAGA+AGG + chr8.1:62193865-62193884 None:intergenic 40.0%
TAAGGAACCATTACGAGTCT+TGG - chr8.1:62194782-62194801 MS.gene063868:CDS 40.0%
TCCATCATTTGCAACAACCT+CGG - chr8.1:62194016-62194035 MS.gene063868:CDS 40.0%
TCTTGGTCCCAAATCTCAAA+AGG - chr8.1:62194274-62194293 MS.gene063868:CDS 40.0%
TGGACTTGGAAAAGTTGCTT+TGG - chr8.1:62194720-62194739 MS.gene063868:CDS 40.0%
TTAAGTAACGGCGTCTTCAT+TGG - chr8.1:62194196-62194215 MS.gene063868:CDS 40.0%
TTCTCAACCAAGACTCGTAA+TGG + chr8.1:62194792-62194811 None:intergenic 40.0%
! ATGGTTTCAAGTGGAAGGTA+TGG + chr8.1:62193833-62193852 None:intergenic 40.0%
! GGGGTACTAATAAGAATCGT+AGG - chr8.1:62194935-62194954 MS.gene063868:CDS 40.0%
! TACAATTGGAGGTGTGTAGT+TGG + chr8.1:62194098-62194117 None:intergenic 40.0%
! TGGAAAGGGTTTGTTTGAGA+TGG + chr8.1:62193910-62193929 None:intergenic 40.0%
! TTTGCATGGTTTCAAGTGGA+AGG + chr8.1:62193838-62193857 None:intergenic 40.0%
!! CAGGTAACGGAGTTAAGTAA+CGG - chr8.1:62194184-62194203 MS.gene063868:CDS 40.0%
!! GTTTGTGGTGTCATTGTGTT+GGG + chr8.1:62193978-62193997 None:intergenic 40.0%
!! TGGTTTGATGGGTTGAGTAT+GGG + chr8.1:62193881-62193900 None:intergenic 40.0%
!! TGTTTGTGGTGTCATTGTGT+TGG + chr8.1:62193979-62193998 None:intergenic 40.0%
!! TTGGTTTGATGGGTTGAGTA+TGG + chr8.1:62193882-62193901 None:intergenic 40.0%
AAAACCAAGTCTAGGAGTCC+AGG - chr8.1:62194165-62194184 MS.gene063868:CDS 45.0%
AAATAATCCTCCTCTAGCGG+TGG + chr8.1:62194643-62194662 None:intergenic 45.0%
AAGTCTAGGAGTCCAGGTAA+CGG - chr8.1:62194171-62194190 MS.gene063868:CDS 45.0%
AATGGGTGCTTGGATTGTAG+TGG + chr8.1:62193750-62193769 None:intergenic 45.0%
AATGGTGTGAGGATGAAAGC+AGG - chr8.1:62194682-62194701 MS.gene063868:CDS 45.0%
ACCGAGGTTGTTGCAAATGA+TGG + chr8.1:62194020-62194039 None:intergenic 45.0%
AGTCTTGGTTGAGAACACCA+AGG - chr8.1:62194797-62194816 MS.gene063868:CDS 45.0%
ATGGTGTGAGGATGAAAGCA+GGG - chr8.1:62194683-62194702 MS.gene063868:CDS 45.0%
ATTACAAGACGCCCTGCAAA+GGG + chr8.1:62193953-62193972 None:intergenic 45.0%
CATTCTTTCCACCCTTTGCA+GGG - chr8.1:62193939-62193958 MS.gene063868:CDS 45.0%
CCGAAATAATCCTCCTCTAG+CGG + chr8.1:62194646-62194665 None:intergenic 45.0%
CCGCTAGAGGAGGATTATTT+CGG - chr8.1:62194643-62194662 MS.gene063868:CDS 45.0%
CGTTACTTAACTCCGTTACC+TGG + chr8.1:62194186-62194205 None:intergenic 45.0%
CTACCACTTGGAAACAACCT+TGG + chr8.1:62194817-62194836 None:intergenic 45.0%
GAGGTGTGTAGTTGGATTTC+AGG + chr8.1:62194090-62194109 None:intergenic 45.0%
GCTACGTCAAAACCAAGTCT+AGG - chr8.1:62194157-62194176 MS.gene063868:CDS 45.0%
GGGAAATGATGCTGAGTTCA+TGG - chr8.1:62195038-62195057 MS.gene063868:CDS 45.0%
GGGAACCAACGTTGAAATGA+AGG + chr8.1:62194322-62194341 None:intergenic 45.0%
TCATTCTTTCCACCCTTTGC+AGG - chr8.1:62193938-62193957 MS.gene063868:CDS 45.0%
TGTTGTTGTCAATGGGTGCT+TGG + chr8.1:62193760-62193779 None:intergenic 45.0%
! AACGGTTGTGTTTTGTGCTG+TGG + chr8.1:62194059-62194078 None:intergenic 45.0%
! ACACCAAGGTTGTTTCCAAG+TGG - chr8.1:62194811-62194830 MS.gene063868:CDS 45.0%
! AGTGGTGGAGATCACTTTGA+GGG + chr8.1:62193732-62193751 None:intergenic 45.0%
! ATGGGTTGAGTATGGGTTTG+TGG + chr8.1:62193874-62193893 None:intergenic 45.0%
! GGATTCTAAGCGTTGTTCTG+AGG + chr8.1:62194407-62194426 None:intergenic 45.0%
! TAGTGGTGGAGATCACTTTG+AGG + chr8.1:62193733-62193752 None:intergenic 45.0%
! TTGCAGACGGCAAGTCATTT+TGG - chr8.1:62194239-62194258 MS.gene063868:CDS 45.0%
!! CAGCACTGATTAATGGTGTG+AGG - chr8.1:62194671-62194690 MS.gene063868:CDS 45.0%
!! GGAAACGCAGCACTGATTAA+TGG - chr8.1:62194664-62194683 MS.gene063868:CDS 45.0%
!! GGTAATTTGTTAGCAACCGG+CGG - chr8.1:62194847-62194866 MS.gene063868:CDS 45.0%
!!! GGGGGAACCTTTTGAGATTT+GGG + chr8.1:62194284-62194303 None:intergenic 45.0%
!!! TGGGGGAACCTTTTGAGATT+TGG + chr8.1:62194285-62194304 None:intergenic 45.0%
AACAAACCACAGAGAACCGC+CGG + chr8.1:62194866-62194885 None:intergenic 50.0%
ACGTATGATTCCACCGCTAG+AGG - chr8.1:62194630-62194649 MS.gene063868:CDS 50.0%
CCAAGTGGTAGTTCTCCAAG+TGG - chr8.1:62194826-62194845 MS.gene063868:CDS 50.0%
CCACTTGGAGAACTACCACT+TGG + chr8.1:62194829-62194848 None:intergenic 50.0%
GATTACAAGACGCCCTGCAA+AGG + chr8.1:62193954-62193973 None:intergenic 50.0%
GTTACCTGGACTCCTAGACT+TGG + chr8.1:62194172-62194191 None:intergenic 50.0%
TATGATTCCACCGCTAGAGG+AGG - chr8.1:62194633-62194652 MS.gene063868:CDS 50.0%
TCTAGCGGTGGAATCATACG+TGG + chr8.1:62194631-62194650 None:intergenic 50.0%
TGCAACCACGTTGTTGCAGA+CGG - chr8.1:62194226-62194245 MS.gene063868:CDS 50.0%
TGGTGTGAGGATGAAAGCAG+GGG - chr8.1:62194684-62194703 MS.gene063868:CDS 50.0%
! GGAAGGTATGGAAGATCCCA+TGG + chr8.1:62193821-62193840 None:intergenic 50.0%
! GTGTATGCTGGTGCTGAAGA+AGG - chr8.1:62194967-62194986 MS.gene063868:CDS 50.0%
! TTGTGTTTTGTGCTGTGGCG+TGG + chr8.1:62194054-62194073 None:intergenic 50.0%
!! GAGGTTCTAGAGTGTGGACT+TGG - chr8.1:62194706-62194725 MS.gene063868:CDS 50.0%
!! TTTTGGTTGGGGAAAGCCTG+TGG - chr8.1:62194900-62194919 MS.gene063868:CDS 50.0%
AAGCCTGTGGCATTTCGACG+TGG - chr8.1:62194913-62194932 MS.gene063868:CDS 55.0%
ACTTGCCGTCTGCAACAACG+TGG + chr8.1:62194234-62194253 None:intergenic 55.0%
AGCCTGTGGCATTTCGACGT+GGG - chr8.1:62194914-62194933 MS.gene063868:CDS 55.0%
GGGTGCTTGGATTGTAGTGG+TGG + chr8.1:62193747-62193766 None:intergenic 55.0%
TGTGAGGATGAAAGCAGGGG+AGG - chr8.1:62194687-62194706 MS.gene063868:CDS 55.0%
!! AGCGTTGTTCTGAGGAGCCT+TGG + chr8.1:62194399-62194418 None:intergenic 55.0%
ACAAGACGCCCTGCAAAGGG+TGG + chr8.1:62193950-62193969 None:intergenic 60.0%
CCTGTGGCATTTCGACGTGG+GGG - chr8.1:62194916-62194935 MS.gene063868:CDS 60.0%
GCCTGTGGCATTTCGACGTG+GGG - chr8.1:62194915-62194934 MS.gene063868:CDS 60.0%
TAGCAACCGGCGGTTCTCTG+TGG - chr8.1:62194857-62194876 MS.gene063868:CDS 60.0%
!! GCAGGGGAGGTTCTAGAGTG+TGG - chr8.1:62194700-62194719 MS.gene063868:CDS 60.0%
CCCCCACGTCGAAATGCCAC+AGG + chr8.1:62194919-62194938 None:intergenic 65.0%
! CGTGGACGAAGAGTGCACCG+AGG + chr8.1:62194036-62194055 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 62193722 62195074 62193722 ID=MS.gene063868
chr8.1 mRNA 62193722 62195074 62193722 ID=MS.gene063868.t1;Parent=MS.gene063868
chr8.1 exon 62193722 62195074 62193722 ID=MS.gene063868.t1.exon1;Parent=MS.gene063868.t1
chr8.1 CDS 62193722 62195074 62193722 ID=cds.MS.gene063868.t1;Parent=MS.gene063868.t1
Gene Sequence

>MS.gene063868

ATGGGTTCCCTCAAAGTGATCTCCACCACTACAATCCAAGCACCCATTGACAACAACAATCATGACTCAATAGATTATCAGAAAATAGAATTAACACCATGGGATCTTCCATACCTTCCACTTGAAACCATGCAAATAGGCCTTCTTTTCCACAAACCCATACTCAACCCATCAAACCAAATCAACCATCTCAAACAAACCCTTTCCACTACTCTATCATTCTTTCCACCCTTTGCAGGGCGTCTTGTAATCACCCAACACAATGACACCACAAACAACAACAACACATCATGTTCCATCATTTGCAACAACCTCGGTGCACTCTTCGTCCACGCCACAGCACAAAACACAACCGTTAACGACATCCTGAAATCCAACTACACACCTCCAATTGTACACTCATTTTTTCCACTTAACGGAGTTAAAAACTACGAAGCTACGTCAAAACCAAGTCTAGGAGTCCAGGTAACGGAGTTAAGTAACGGCGTCTTCATTGGCTTCGCATGCAACCACGTTGTTGCAGACGGCAAGTCATTTTGGCATTTCGTTAATTCTTGGTCCCAAATCTCAAAAGGTTCCCCCAAAATAACAAAACTCCCTTCATTTCAACGTTGGTTCCCAAACGATATTGAAATTCCAATACGCTTTCCTTTCATAAAAACAGAATCACAAAACCAAGGCTCCTCAGAACAACGCTTAGAATCCAAGTTAGCCGAAAGAATCTTCCATTTCACAAAGGAGAAAATTGCCGAATTGAAATCAAAAGCAAATTTAGAAGCTGGAACAGACATAACTAAAATATCTTCGCTTCAAGCGTTATTATCTCACGTTTGGCGTTCTGTTGTTCGCTGCCAAAAACTTGATCCACAAGAAGATTATTTCCATGTGTTGAGTATAGGTGTTAGACCACGTATGATTCCACCGCTAGAGGAGGATTATTTCGGAAACGCAGCACTGATTAATGGTGTGAGGATGAAAGCAGGGGAGGTTCTAGAGTGTGGACTTGGAAAAGTTGCTTTGGAGATAAACAAGATAATTTCCTTACATTCTGATGAGAAAATAAGGAACCATTACGAGTCTTGGTTGAGAACACCAAGGTTGTTTCCAAGTGGTAGTTCTCCAAGTGGTAATTTGTTAGCAACCGGCGGTTCTCTGTGGTTTGTTGTTTTTGGCAATGATTTTGGTTGGGGAAAGCCTGTGGCATTTCGACGTGGGGGTACTAATAAGAATCGTAGGAGAATTATGGTGTATGCTGGTGCTGAAGAAGGTAGTATAGATATTGAAGTTTGTCTTTCTTATGAGATTTTGGAGGCTTTGGGAAATGATGCTGAGTTCATGGTTCCCAGTTCTTAA
Protein sequence

>MS.gene063868.t1

MGSLKVISTTTIQAPIDNNNHDSIDYQKIELTPWDLPYLPLETMQIGLLFHKPILNPSNQINHLKQTLSTTLSFFPPFAGRLVITQHNDTTNNNNTSCSIICNNLGALFVHATAQNTTVNDILKSNYTPPIVHSFFPLNGVKNYEATSKPSLGVQVTELSNGVFIGFACNHVVADGKSFWHFVNSWSQISKGSPKITKLPSFQRWFPNDIEIPIRFPFIKTESQNQGSSEQRLESKLAERIFHFTKEKIAELKSKANLEAGTDITKISSLQALLSHVWRSVVRCQKLDPQEDYFHVLSIGVRPRMIPPLEEDYFGNAALINGVRMKAGEVLECGLGKVALEINKIISLHSDEKIRNHYESWLRTPRLFPSGSSPSGNLLATGGSLWFVVFGNDFGWGKPVAFRRGGTNKNRRRIMVYAGAEEGSIDIEVCLSYEILEALGNDAEFMVPSS