AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56797

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56797.t1 MTR_4g094828 90.508 453 41 2 1 453 1 451 0.0 848
MS.gene56797.t1 MTR_4g022290 84.071 452 61 3 1 450 1 443 0.0 771
MS.gene56797.t1 MTR_8g079710 74.779 452 99 7 1 448 1 441 0.0 668
MS.gene56797.t1 MTR_4g094835 55.188 453 179 12 1 444 1 438 3.60e-169 483
MS.gene56797.t1 MTR_4g094842 54.267 457 179 10 1 444 1 440 2.22e-160 461
MS.gene56797.t1 MTR_0190s0020 50.219 456 209 5 1 452 1 442 1.59e-153 443
MS.gene56797.t1 MTR_3g088420 51.316 456 195 11 1 452 1 433 1.50e-151 438
MS.gene56797.t1 MTR_4g022800 50.756 463 196 12 1 452 1 442 8.28e-149 431
MS.gene56797.t1 MTR_4g094838 50.885 452 179 11 6 444 3 424 1.89e-148 430
MS.gene56797.t1 MTR_4g022825 51.092 458 198 12 1 451 1 439 1.13e-147 428
MS.gene56797.t1 MTR_4g022720 50.547 457 199 12 1 452 1 435 2.11e-147 427
MS.gene56797.t1 MTR_3g026310 50.107 467 199 11 1 452 1 448 5.21e-147 427
MS.gene56797.t1 MTR_8g041770 49.782 458 207 9 1 452 1 441 1.12e-145 423
MS.gene56797.t1 MTR_7g026860 50.108 461 198 13 1 452 1 438 4.22e-144 419
MS.gene56797.t1 MTR_4g022670 49.783 460 204 11 1 452 1 441 6.64e-143 416
MS.gene56797.t1 MTR_4g094832 54.974 382 146 9 73 444 57 422 7.18e-138 403
MS.gene56797.t1 MTR_4g022760 44.009 459 188 10 1 452 1 397 6.14e-118 351
MS.gene56797.t1 MTR_6g016815 41.410 454 177 10 1 452 15 381 1.72e-103 313
MS.gene56797.t1 MTR_3g101900 37.443 438 243 12 29 453 30 449 4.33e-81 258
MS.gene56797.t1 MTR_3g101930 37.415 441 236 13 25 444 26 447 2.28e-80 256
MS.gene56797.t1 MTR_3g102020 36.943 471 248 16 6 453 8 452 5.06e-79 253
MS.gene56797.t1 MTR_3g102010 36.889 450 240 14 25 453 26 452 1.65e-78 251
MS.gene56797.t1 MTR_3g101970 35.762 453 232 14 23 453 23 438 8.95e-77 246
MS.gene56797.t1 MTR_3g101960 36.486 444 236 13 23 444 23 442 1.11e-76 246
MS.gene56797.t1 MTR_8g040760 54.378 217 91 4 1 212 1 214 3.25e-71 224
MS.gene56797.t1 MTR_5g081960 32.520 492 258 13 6 444 8 478 1.70e-65 218
MS.gene56797.t1 MTR_3g070170 32.543 464 263 12 13 444 17 462 7.35e-64 213
MS.gene56797.t1 MTR_4g094825 56.085 189 75 5 20 202 83 269 1.68e-62 207
MS.gene56797.t1 MTR_4g094825 50.685 146 58 4 300 444 251 383 1.62e-36 138
MS.gene56797.t1 MTR_4g015180 31.781 494 262 14 8 444 4 479 6.68e-61 206
MS.gene56797.t1 MTR_7g026850 60.526 152 58 2 34 184 12 162 1.19e-58 191
MS.gene56797.t1 MTR_8g040780 45.662 219 104 7 230 444 17 224 2.56e-50 171
MS.gene56797.t1 MTR_6g034495 66.379 116 37 1 70 183 45 160 1.02e-46 159
MS.gene56797.t1 MTR_3g102030 32.861 353 171 14 70 405 17 320 1.72e-43 155
MS.gene56797.t1 MTR_6g045553 28.700 446 275 11 29 444 35 467 1.56e-38 145
MS.gene56797.t1 MTR_7g026770 41.885 191 77 5 146 335 132 289 5.81e-35 132
MS.gene56797.t1 MTR_7g026830 68.293 82 26 0 89 170 16 97 6.77e-34 123
MS.gene56797.t1 MTR_6g034485 40.288 139 69 3 191 329 1 125 5.82e-27 105
MS.gene56797.t1 MTR_8g075610 25.823 395 255 15 59 442 47 414 3.37e-26 110
MS.gene56797.t1 MTR_2g105330 26.329 414 262 16 46 442 39 426 3.60e-25 107
MS.gene56797.t1 MTR_2g064495 26.570 414 261 17 46 442 39 426 4.66e-25 107
MS.gene56797.t1 MTR_4g007540 25.971 412 269 16 46 443 39 428 9.24e-25 106
MS.gene56797.t1 MTR_3g049150 24.171 422 264 15 46 437 38 433 4.60e-22 98.6
MS.gene56797.t1 MTR_7g027175 26.119 402 264 14 49 443 58 433 6.82e-22 98.2
MS.gene56797.t1 MTR_8g096530 27.095 358 211 14 59 400 59 382 7.37e-22 97.8
MS.gene56797.t1 MTR_3g026950 24.762 420 268 16 46 437 38 437 9.27e-22 97.8
MS.gene56797.t1 MTR_3g098980 24.937 397 252 16 59 442 66 429 5.02e-21 95.5
MS.gene56797.t1 MTR_5g026320 26.383 470 271 22 6 444 20 445 7.80e-21 95.1
MS.gene56797.t1 MTR_7g085990 24.279 416 283 11 49 442 45 450 6.24e-20 92.4
MS.gene56797.t1 MTR_1g040175 24.044 366 246 12 45 401 45 387 1.10e-19 91.7
MS.gene56797.t1 MTR_1g040195 24.163 418 272 14 31 439 24 405 3.45e-18 87.0
MS.gene56797.t1 MTR_7g014200 23.465 456 281 15 27 439 28 458 1.06e-17 85.5
MS.gene56797.t1 MTR_7g014160 24.053 449 274 15 29 433 30 455 3.98e-17 84.0
MS.gene56797.t1 MTR_7g014250 24.450 409 247 16 33 404 30 413 5.13e-17 83.6
MS.gene56797.t1 MTR_4g097090 22.936 436 248 17 46 433 45 440 7.61e-17 82.8
MS.gene56797.t1 MTR_7g014610 23.077 468 274 18 32 443 32 469 6.53e-16 80.1
MS.gene56797.t1 MTR_3g087640 23.651 482 291 17 5 439 11 462 9.49e-16 79.7
MS.gene56797.t1 MTR_6g015320 21.564 422 266 14 24 403 11 409 1.26e-15 79.3
MS.gene56797.t1 MTR_1g081660 24.069 403 239 16 29 401 31 396 2.95e-15 78.2
MS.gene56797.t1 MTR_7g014230 25.060 415 249 17 27 404 26 415 4.10e-15 77.8
MS.gene56797.t1 MTR_6g015815 23.558 416 247 16 27 403 32 415 5.46e-15 77.4
MS.gene56797.t1 MTR_5g090810 23.573 403 244 14 37 401 36 412 7.52e-15 77.0
MS.gene56797.t1 MTR_2g089670 25.445 393 239 17 41 400 58 429 7.87e-15 77.0
MS.gene56797.t1 MTR_4g127650 23.192 401 256 18 59 433 66 440 8.68e-15 76.6
MS.gene56797.t1 MTR_6g015830 24.390 410 247 14 29 403 34 415 1.01e-14 76.3
MS.gene56797.t1 MTR_8g012750 24.038 416 259 17 26 400 23 422 1.29e-14 76.3
MS.gene56797.t1 MTR_3g112180 22.967 418 288 13 33 442 38 429 2.20e-14 75.5
MS.gene56797.t1 MTR_6g015850 23.632 402 261 12 27 403 32 412 1.47e-13 72.8
MS.gene56797.t1 MTR_3g028210 22.870 446 295 16 21 434 21 449 2.75e-13 72.0
MS.gene56797.t1 MTR_7g114490 23.864 440 277 18 31 442 25 434 4.12e-13 71.6
MS.gene56797.t1 MTR_7g014620 22.727 440 265 16 29 433 15 414 5.36e-13 70.9
MS.gene56797.t1 MTR_7g013850 21.369 482 285 17 32 449 32 483 5.63e-13 71.2
MS.gene56797.t1 MTR_6g053810 25.649 308 207 11 59 360 71 362 1.03e-12 70.1
MS.gene56797.t1 MTR_7g026780 38.053 113 43 4 340 452 26 111 1.60e-12 64.3
MS.gene56797.t1 MTR_7g114520 23.192 401 261 16 31 408 26 402 1.71e-12 69.3
MS.gene56797.t1 MTR_7g056087 22.951 427 278 19 37 439 30 429 3.31e-12 68.6
MS.gene56797.t1 MTR_7g114550 28.326 233 142 9 31 247 25 248 4.97e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56797.t1 AT5G67150 38.312 462 254 13 4 453 5 447 3.23e-95 294
MS.gene56797.t1 AT3G50270 37.086 453 259 13 2 444 4 440 2.44e-87 274
MS.gene56797.t1 AT5G67160 38.173 427 230 12 25 444 25 424 4.75e-85 268
MS.gene56797.t1 AT3G50300 37.607 468 244 15 1 443 1 445 2.06e-84 266
MS.gene56797.t1 AT5G38130 36.569 443 250 11 1 433 21 442 1.57e-80 257
MS.gene56797.t1 AT3G50280 34.590 451 272 10 1 444 1 435 2.55e-80 256
MS.gene56797.t1 AT5G42830 35.308 439 252 12 29 453 30 450 4.47e-76 245
MS.gene56797.t1 AT5G07860 36.364 429 244 10 29 445 31 442 5.10e-73 237
MS.gene56797.t1 AT5G07850 34.773 440 254 12 29 452 31 453 1.83e-71 233
MS.gene56797.t1 AT5G07870 35.666 443 254 12 29 452 31 461 4.80e-68 224
MS.gene56797.t1 AT2G39980 32.059 471 258 12 19 444 19 472 3.68e-67 223
MS.gene56797.t1 AT5G01210 32.680 459 256 12 22 444 20 461 1.51e-65 218
MS.gene56797.t1 AT3G50280 35.423 319 188 7 130 444 1 305 5.08e-56 188
MS.gene56797.t1 AT3G50290 38.790 281 154 7 124 398 1 269 1.29e-50 173
MS.gene56797.t1 AT5G23940 31.724 435 265 11 25 441 21 441 3.99e-48 172
MS.gene56797.t1 AT5G41040 27.114 402 252 16 59 448 69 441 7.79e-27 112
MS.gene56797.t1 AT5G41040 27.160 405 248 16 59 448 85 457 8.00e-27 112
MS.gene56797.t1 AT3G48720 26.492 419 263 17 39 442 37 425 6.30e-26 109
MS.gene56797.t1 AT5G63560 25.128 390 241 13 59 434 61 413 2.83e-24 105
MS.gene56797.t1 AT5G48930 26.456 412 247 18 46 433 39 418 2.25e-23 102
MS.gene56797.t1 AT2G19070 26.742 445 270 21 29 442 25 444 7.84e-22 98.2
MS.gene56797.t1 AT3G29590 22.378 429 301 13 27 439 26 438 4.94e-17 83.6
MS.gene56797.t1 AT5G47950 25.871 402 237 15 55 443 57 410 5.00e-17 83.2
MS.gene56797.t1 AT1G78990 24.932 369 234 14 48 401 56 396 5.45e-17 83.6
MS.gene56797.t1 AT5G39080 23.877 423 272 17 13 403 12 416 7.73e-17 83.2
MS.gene56797.t1 AT1G32910 24.725 364 224 13 59 401 65 399 1.23e-16 82.4
MS.gene56797.t1 AT3G26040 26.024 415 240 15 53 445 53 422 8.35e-16 79.7
MS.gene56797.t1 AT5G23970 24.057 424 250 17 45 444 38 413 1.23e-15 79.0
MS.gene56797.t1 AT3G03480 24.605 443 267 20 31 441 34 441 1.48e-15 79.0
MS.gene56797.t1 AT1G65450 25.000 356 230 16 57 400 70 400 2.61e-15 78.2
MS.gene56797.t1 AT3G29670 21.798 445 315 13 6 433 5 433 3.37e-15 77.8
MS.gene56797.t1 AT2G40230 24.750 400 258 15 20 403 17 389 7.45e-15 76.6
MS.gene56797.t1 AT5G07080 24.000 400 253 14 59 438 70 438 1.44e-14 75.9
MS.gene56797.t1 AT5G16410 23.515 404 243 16 24 401 56 419 5.49e-14 74.3
MS.gene56797.t1 AT1G24420 25.217 345 208 15 70 403 76 381 2.43e-13 72.0
MS.gene56797.t1 AT1G24420 25.217 345 208 15 70 403 76 381 2.43e-13 72.0
MS.gene56797.t1 AT5G47980 23.671 414 246 16 58 445 60 429 1.30e-12 69.7
MS.gene56797.t1 AT5G39090 23.058 412 269 16 13 400 12 399 3.80e-11 65.5

Find 94 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCTTCCTTATGATATTT+TGG 0.223517 8.2:+25484493 MS.gene56797:CDS
TCAAAAGCTAATGCAGAAAT+TGG 0.278916 8.2:+25483942 MS.gene56797:CDS
AATGCAAGTAGGGGAATTAA+TGG 0.309600 8.2:+25484175 MS.gene56797:CDS
GGATCTCAAATATCTTAAAA+TGG 0.314276 8.2:+25483296 MS.gene56797:CDS
AATTTCTTTGGGAGTGGAAT+TGG 0.318710 8.2:+25484134 MS.gene56797:CDS
AAGTTAGAAGAGAATTTCTT+TGG 0.321338 8.2:+25484122 MS.gene56797:CDS
ATGGTCGATTTGGTTTGTTT+TGG 0.322357 8.2:-25483352 None:intergenic
AAGAAATTCTCTTCTAACTT+TGG 0.335900 8.2:-25484119 None:intergenic
TCGTGTTGCCTGTAGATTGT+AGG 0.344320 8.2:+25484084 MS.gene56797:CDS
ACACCATTTCTAACTGCAAT+AGG 0.350294 8.2:-25484398 None:intergenic
GTGTAGACGCCAAGTCATTT+TGG 0.357778 8.2:+25483694 MS.gene56797:CDS
TTAATCGATGGTATCTTCAT+TGG 0.379145 8.2:+25483651 MS.gene56797:CDS
ATGGAACATGAGGCGTTGTT+AGG 0.379377 8.2:-25483453 None:intergenic
ATGGGACGGTTAATGTAGTT+TGG 0.381796 8.2:-25483795 None:intergenic
TTTGTTTAAGATGGTCGATT+TGG 0.383044 8.2:-25483362 None:intergenic
TCACCACGGTTCAATTATTA+CGG 0.389485 8.2:+25484353 MS.gene56797:CDS
TTACGGTAATGACTTTGGTT+GGG 0.403096 8.2:+25484370 MS.gene56797:CDS
GGGAGTGGAATTGGAGCAAC+TGG 0.414076 8.2:+25484143 MS.gene56797:CDS
TTTGGCATTTCATTAACTCT+TGG 0.420403 8.2:+25483712 MS.gene56797:CDS
AAAATGGCACCAATCCAAAA+AGG 0.433344 8.2:+25483312 MS.gene56797:CDS
ACGAAAGCAGACCTTTCTTC+TGG 0.437020 8.2:-25484065 None:intergenic
AATTATTACGGTAATGACTT+TGG 0.438421 8.2:+25484365 MS.gene56797:CDS
TTGGCATTTCATTAACTCTT+GGG 0.439646 8.2:+25483713 MS.gene56797:CDS
CTTTGTGAAAGGAAACTGTA+TGG 0.444172 8.2:-25483814 None:intergenic
TGGAAAGTGTTTGTTTAAGA+TGG 0.447960 8.2:-25483371 None:intergenic
GAAGAGAATTTCTTTGGGAG+TGG 0.449897 8.2:+25484128 MS.gene56797:CDS
ATTACGGTAATGACTTTGGT+TGG 0.453916 8.2:+25484369 MS.gene56797:CDS
GATATGTAATAATGCAAGTA+GGG 0.455704 8.2:+25484165 MS.gene56797:CDS
AGTTAGAAGAGAATTTCTTT+GGG 0.462850 8.2:+25484123 MS.gene56797:CDS
TACGGTAATGACTTTGGTTG+GGG 0.464169 8.2:+25484371 MS.gene56797:CDS
ATCAAAATCTCATCATGAAA+AGG 0.465218 8.2:+25484244 MS.gene56797:CDS
GGAAACCAACGTTCGAAAGA+AGG 0.465248 8.2:-25483774 None:intergenic
TCGAATCTTCCACTTTACTA+AGG 0.472625 8.2:+25483899 MS.gene56797:CDS
GAGGAACATACTTTGGTTGA+AGG 0.473999 8.2:-25483542 None:intergenic
AAGCCTATTGCAGTTAGAAA+TGG 0.478020 8.2:+25484395 MS.gene56797:CDS
TACCTGGATCCGCTATAGAC+GGG 0.488932 8.2:-25484298 None:intergenic
AAGAATAATGACGAAACAAT+GGG 0.494772 8.2:-25483564 None:intergenic
ATAGCCTCCAAAATATCATA+AGG 0.495132 8.2:-25484500 None:intergenic
AACTTCTTTCCACCACTCGC+TGG 0.495410 8.2:+25483402 MS.gene56797:CDS
GAATTAATGGAAAGTGGGAT+TGG 0.504651 8.2:+25484188 MS.gene56797:CDS
GGTAGAGTTATTGTGCTTGG+TGG 0.507052 8.2:+25484440 MS.gene56797:CDS
ACAATGGGAGGAACATACTT+TGG 0.509541 8.2:-25483549 None:intergenic
GAATTTGTTCCTTAGTAAAG+TGG 0.513443 8.2:-25483908 None:intergenic
GGATATGTAATAATGCAAGT+AGG 0.520472 8.2:+25484164 MS.gene56797:CDS
TTAAGATATTTGAGATCCCA+TGG 0.520479 8.2:-25483291 None:intergenic
AAATCCCTTCTTTCGAACGT+TGG 0.525283 8.2:+25483769 MS.gene56797:CDS
TACTTATAATTAAACGCCAA+AGG 0.529613 8.2:-25484028 None:intergenic
ATTCTTGTCCTACAATCTAC+AGG 0.533011 8.2:-25484092 None:intergenic
TAAGAATAATGACGAAACAA+TGG 0.534494 8.2:-25483565 None:intergenic
GTAGGGGAATTAATGGAAAG+TGG 0.534728 8.2:+25484182 MS.gene56797:CDS
GAAACCAACGTTCGAAAGAA+GGG 0.538266 8.2:-25483773 None:intergenic
AAAATCTTAAACCAGAAGAA+AGG 0.538946 8.2:+25484054 MS.gene56797:CDS
AAATTACTTGATGATCTACC+TGG 0.540361 8.2:-25484314 None:intergenic
ATTGGTAGAGTTATTGTGCT+TGG 0.541398 8.2:+25484437 MS.gene56797:CDS
ACCCCGTCTATAGCGGATCC+AGG 0.542175 8.2:+25484296 MS.gene56797:CDS
GATTCACTCAACACCATGGC+AGG 0.542716 8.2:+25483186 None:intergenic
TTAGACAGCCATAGCATCCA+TGG 0.543828 8.2:-25484540 None:intergenic
CTACCTGGATCCGCTATAGA+CGG 0.544068 8.2:-25484299 None:intergenic
GTTGGACTTAAAAGAAACAT+TGG 0.549002 8.2:+25484419 MS.gene56797:CDS
TAGGGGAATTAATGGAAAGT+GGG 0.552463 8.2:+25484183 MS.gene56797:CDS
AATTATCGATTTAGCACCAT+GGG 0.552815 8.2:+25483275 MS.gene56797:CDS
ACCAGCGTTGTTGCAAATGA+TGG 0.553843 8.2:-25483472 None:intergenic
GTTACTTGTACTGCAAGTAA+TGG 0.555096 8.2:-25483624 None:intergenic
CATACAGTTTCCTTTCACAA+AGG 0.556469 8.2:+25483815 MS.gene56797:CDS
ATACTTTGGTTGAAGGATGT+CGG 0.566713 8.2:-25483535 None:intergenic
TGGGATTGGTAAAATTGCAA+TGG 0.567200 8.2:+25484202 MS.gene56797:CDS
TCATTTGTCCATGGATGCTA+TGG 0.571556 8.2:+25484532 MS.gene56797:CDS
AACGAAGTGCAAAACTATGA+AGG 0.575652 8.2:+25483591 MS.gene56797:CDS
GTGCTTGGTGGTGCAGAAGA+AGG 0.576706 8.2:+25484452 MS.gene56797:CDS
ATTGCAGTTAGAAATGGTGT+TGG 0.578320 8.2:+25484401 MS.gene56797:CDS
TGGTTGAAGGATGTCGGCAA+TGG 0.578913 8.2:-25483529 None:intergenic
TCCATCATTTGCAACAACGC+TGG 0.580870 8.2:+25483471 MS.gene56797:CDS
TAGTGAGTAACTATGAGTCT+TGG 0.585199 8.2:+25484267 MS.gene56797:CDS
TGGAAAGAAGTTGAGAGTGT+TGG 0.586632 8.2:-25483391 None:intergenic
TTTGTGAAAGGAAACTGTAT+GGG 0.590779 8.2:-25483813 None:intergenic
GGAAAATGATCATTTGTCCA+TGG 0.594943 8.2:+25484523 MS.gene56797:CDS
ACAATCCGAGCAACAAAACA+TGG 0.599813 8.2:+25483231 MS.gene56797:CDS
AATGAAATGCCAAAATGACT+TGG 0.602712 8.2:-25483703 None:intergenic
GTTGAAAACCCCGTCTATAG+CGG 0.608414 8.2:+25484289 MS.gene56797:CDS
GATAAGTAGAATGCCTGCCA+TGG 0.611905 8.2:-25483199 None:intergenic
TGAAAGGAAACTGTATGGGA+CGG 0.613243 8.2:-25483809 None:intergenic
ACAAGACGACCAGCGAGTGG+TGG 0.630223 8.2:-25483411 None:intergenic
CAAGTAACAGAGTTAATCGA+TGG 0.631905 8.2:+25483639 MS.gene56797:CDS
AATTGCAATGGAAATGAACA+AGG 0.638167 8.2:+25484214 MS.gene56797:CDS
ACCTGGATCCGCTATAGACG+GGG 0.647165 8.2:-25484297 None:intergenic
AAATTATCGATTTAGCACCA+TGG 0.647404 8.2:+25483274 MS.gene56797:CDS
ATATGTAATAATGCAAGTAG+GGG 0.680188 8.2:+25484166 MS.gene56797:CDS
ACTTGGCGTCTACACCCACA+TGG 0.689659 8.2:-25483686 None:intergenic
TCTTGGGCAGAAATCGCACA+AGG 0.690880 8.2:+25483729 MS.gene56797:CDS
ATTACAAGACGACCAGCGAG+TGG 0.695572 8.2:-25483414 None:intergenic
GTTGCAAATGATGGAACATG+AGG 0.705156 8.2:-25483463 None:intergenic
TGATAGCAAGCAGTTCACCA+CGG 0.707603 8.2:+25484339 MS.gene56797:CDS
AATAATGACGAAACAATGGG+AGG 0.713480 8.2:-25483561 None:intergenic
TTACCGTAATAATTGAACCG+TGG 0.738497 8.2:-25484356 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAATCTTAAACCAGAAGAA+AGG + chr8.2:25484054-25484073 MS.gene56797:CDS 25.0%
! AAGAAATTCTCTTCTAACTT+TGG - chr8.2:25484122-25484141 None:intergenic 25.0%
! AAGAATAATGACGAAACAAT+GGG - chr8.2:25483567-25483586 None:intergenic 25.0%
! AAGTTAGAAGAGAATTTCTT+TGG + chr8.2:25484122-25484141 MS.gene56797:CDS 25.0%
! AATTATTACGGTAATGACTT+TGG + chr8.2:25484365-25484384 MS.gene56797:CDS 25.0%
! AGTTAGAAGAGAATTTCTTT+GGG + chr8.2:25484123-25484142 MS.gene56797:CDS 25.0%
! ATATGTAATAATGCAAGTAG+GGG + chr8.2:25484166-25484185 MS.gene56797:CDS 25.0%
! ATCAAAATCTCATCATGAAA+AGG + chr8.2:25484244-25484263 MS.gene56797:CDS 25.0%
! GATATGTAATAATGCAAGTA+GGG + chr8.2:25484165-25484184 MS.gene56797:CDS 25.0%
! GGATCTCAAATATCTTAAAA+TGG + chr8.2:25483296-25483315 MS.gene56797:CDS 25.0%
! TAAGAATAATGACGAAACAA+TGG - chr8.2:25483568-25483587 None:intergenic 25.0%
! TACTTATAATTAAACGCCAA+AGG - chr8.2:25484031-25484050 None:intergenic 25.0%
!! TTGTCTTCCTTATGATATTT+TGG + chr8.2:25484493-25484512 MS.gene56797:CDS 25.0%
AATGAAATGCCAAAATGACT+TGG - chr8.2:25483706-25483725 None:intergenic 30.0%
AATTGCAATGGAAATGAACA+AGG + chr8.2:25484214-25484233 MS.gene56797:CDS 30.0%
ATAGCCTCCAAAATATCATA+AGG - chr8.2:25484503-25484522 None:intergenic 30.0%
GGATATGTAATAATGCAAGT+AGG + chr8.2:25484164-25484183 MS.gene56797:CDS 30.0%
GTTGGACTTAAAAGAAACAT+TGG + chr8.2:25484419-25484438 MS.gene56797:CDS 30.0%
TCAAAAGCTAATGCAGAAAT+TGG + chr8.2:25483942-25483961 MS.gene56797:CDS 30.0%
TTAATCGATGGTATCTTCAT+TGG + chr8.2:25483651-25483670 MS.gene56797:CDS 30.0%
TTGGCATTTCATTAACTCTT+GGG + chr8.2:25483713-25483732 MS.gene56797:CDS 30.0%
TTTGGCATTTCATTAACTCT+TGG + chr8.2:25483712-25483731 MS.gene56797:CDS 30.0%
TTTGTGAAAGGAAACTGTAT+GGG - chr8.2:25483816-25483835 None:intergenic 30.0%
! AAAAGAAGACCTTTTTGGAT+TGG - chr8.2:25483324-25483343 None:intergenic 30.0%
! AAATTACTTGATGATCTACC+TGG - chr8.2:25484317-25484336 None:intergenic 30.0%
! GAATTTGTTCCTTAGTAAAG+TGG - chr8.2:25483911-25483930 None:intergenic 30.0%
! TATGGAAAAGAAGACCTTTT+TGG - chr8.2:25483329-25483348 None:intergenic 30.0%
! TTAAGATATTTGAGATCCCA+TGG - chr8.2:25483294-25483313 None:intergenic 30.0%
! TTTGTTTAAGATGGTCGATT+TGG - chr8.2:25483365-25483384 None:intergenic 30.0%
! TTTTGTGCTTCATTTGTGAA+AGG - chr8.2:25483855-25483874 None:intergenic 30.0%
!! AAATTATCGATTTAGCACCA+TGG + chr8.2:25483274-25483293 MS.gene56797:CDS 30.0%
!! AATTATCGATTTAGCACCAT+GGG + chr8.2:25483275-25483294 MS.gene56797:CDS 30.0%
!! TCTTCCTTATGATATTTTGG+AGG + chr8.2:25484496-25484515 MS.gene56797:CDS 30.0%
!! TGGAAAGTGTTTGTTTAAGA+TGG - chr8.2:25483374-25483393 None:intergenic 30.0%
!! TTATGATATTTTGGAGGCTA+TGG + chr8.2:25484502-25484521 MS.gene56797:CDS 30.0%
!!! TTTGGTTTGTTTTGGGTTTA+TGG - chr8.2:25483347-25483366 None:intergenic 30.0%
AAAATGGCACCAATCCAAAA+AGG + chr8.2:25483312-25483331 MS.gene56797:CDS 35.0%
AACGAAGTGCAAAACTATGA+AGG + chr8.2:25483591-25483610 MS.gene56797:CDS 35.0%
AAGCCTATTGCAGTTAGAAA+TGG + chr8.2:25484395-25484414 MS.gene56797:CDS 35.0%
AATAATGACGAAACAATGGG+AGG - chr8.2:25483564-25483583 None:intergenic 35.0%
AATGCAAGTAGGGGAATTAA+TGG + chr8.2:25484175-25484194 MS.gene56797:CDS 35.0%
ACACCATTTCTAACTGCAAT+AGG - chr8.2:25484401-25484420 None:intergenic 35.0%
ATACTTTGGTTGAAGGATGT+CGG - chr8.2:25483538-25483557 None:intergenic 35.0%
ATTACGGTAATGACTTTGGT+TGG + chr8.2:25484369-25484388 MS.gene56797:CDS 35.0%
ATTCTTGTCCTACAATCTAC+AGG - chr8.2:25484095-25484114 None:intergenic 35.0%
ATTGCAGTTAGAAATGGTGT+TGG + chr8.2:25484401-25484420 MS.gene56797:CDS 35.0%
ATTGGTAGAGTTATTGTGCT+TGG + chr8.2:25484437-25484456 MS.gene56797:CDS 35.0%
CAAGTAACAGAGTTAATCGA+TGG + chr8.2:25483639-25483658 MS.gene56797:CDS 35.0%
CATACAGTTTCCTTTCACAA+AGG + chr8.2:25483815-25483834 MS.gene56797:CDS 35.0%
CTTTGTGAAAGGAAACTGTA+TGG - chr8.2:25483817-25483836 None:intergenic 35.0%
GAATTAATGGAAAGTGGGAT+TGG + chr8.2:25484188-25484207 MS.gene56797:CDS 35.0%
GGAAAATGATCATTTGTCCA+TGG + chr8.2:25484523-25484542 MS.gene56797:CDS 35.0%
GTTACTTGTACTGCAAGTAA+TGG - chr8.2:25483627-25483646 None:intergenic 35.0%
TAGGGGAATTAATGGAAAGT+GGG + chr8.2:25484183-25484202 MS.gene56797:CDS 35.0%
TAGTGAGTAACTATGAGTCT+TGG + chr8.2:25484267-25484286 MS.gene56797:CDS 35.0%
TCACCACGGTTCAATTATTA+CGG + chr8.2:25484353-25484372 MS.gene56797:CDS 35.0%
TCGAATCTTCCACTTTACTA+AGG + chr8.2:25483899-25483918 MS.gene56797:CDS 35.0%
TTACCGTAATAATTGAACCG+TGG - chr8.2:25484359-25484378 None:intergenic 35.0%
TTACGGTAATGACTTTGGTT+GGG + chr8.2:25484370-25484389 MS.gene56797:CDS 35.0%
! AATTTCTTTGGGAGTGGAAT+TGG + chr8.2:25484134-25484153 MS.gene56797:CDS 35.0%
! GGTTTTGCAATTAACCATGT+GGG + chr8.2:25483672-25483691 MS.gene56797:CDS 35.0%
! TCGAAAGAAGGGATTTTTGT+TGG - chr8.2:25483765-25483784 None:intergenic 35.0%
! TGGGATTGGTAAAATTGCAA+TGG + chr8.2:25484202-25484221 MS.gene56797:CDS 35.0%
! TTTTGTGCTTCCTTTGTGAA+AGG - chr8.2:25483828-25483847 None:intergenic 35.0%
!! AAGCACTTTTAAGTCACCTT+TGG + chr8.2:25484012-25484031 MS.gene56797:CDS 35.0%
!! AATGGTAGTGTTTTCAGCTT+TGG - chr8.2:25483514-25483533 None:intergenic 35.0%
!! TGGTTTTGCAATTAACCATG+TGG + chr8.2:25483671-25483690 MS.gene56797:CDS 35.0%
!!! ATGGTCGATTTGGTTTGTTT+TGG - chr8.2:25483355-25483374 None:intergenic 35.0%
!!! TGGTCGATTTGGTTTGTTTT+GGG - chr8.2:25483354-25483373 None:intergenic 35.0%
AAATCCCTTCTTTCGAACGT+TGG + chr8.2:25483769-25483788 MS.gene56797:CDS 40.0%
ACAATCCGAGCAACAAAACA+TGG + chr8.2:25483231-25483250 MS.gene56797:CDS 40.0%
ACAATGGGAGGAACATACTT+TGG - chr8.2:25483552-25483571 None:intergenic 40.0%
ATGGGACGGTTAATGTAGTT+TGG - chr8.2:25483798-25483817 None:intergenic 40.0%
GAAACCAACGTTCGAAAGAA+GGG - chr8.2:25483776-25483795 None:intergenic 40.0%
GAGGAACATACTTTGGTTGA+AGG - chr8.2:25483545-25483564 None:intergenic 40.0%
GTAGGGGAATTAATGGAAAG+TGG + chr8.2:25484182-25484201 MS.gene56797:CDS 40.0%
TACGGTAATGACTTTGGTTG+GGG + chr8.2:25484371-25484390 MS.gene56797:CDS 40.0%
TCATTTGTCCATGGATGCTA+TGG + chr8.2:25484532-25484551 MS.gene56797:CDS 40.0%
TGAAAGGAAACTGTATGGGA+CGG - chr8.2:25483812-25483831 None:intergenic 40.0%
! GAAGAGAATTTCTTTGGGAG+TGG + chr8.2:25484128-25484147 MS.gene56797:CDS 40.0%
! GTTGCAAATGATGGAACATG+AGG - chr8.2:25483466-25483485 None:intergenic 40.0%
! TGGAAAGAAGTTGAGAGTGT+TGG - chr8.2:25483394-25483413 None:intergenic 40.0%
!! CATCACCATGTTTTGTTGCT+CGG - chr8.2:25483239-25483258 None:intergenic 40.0%
ACCAGCGTTGTTGCAAATGA+TGG - chr8.2:25483475-25483494 None:intergenic 45.0%
ACGAAAGCAGACCTTTCTTC+TGG - chr8.2:25484068-25484087 None:intergenic 45.0%
GGAAACCAACGTTCGAAAGA+AGG - chr8.2:25483777-25483796 None:intergenic 45.0%
GGTAGAGTTATTGTGCTTGG+TGG + chr8.2:25484440-25484459 MS.gene56797:CDS 45.0%
GTTGAAAACCCCGTCTATAG+CGG + chr8.2:25484289-25484308 MS.gene56797:CDS 45.0%
TCCATCATTTGCAACAACGC+TGG + chr8.2:25483471-25483490 MS.gene56797:CDS 45.0%
TCGTGTTGCCTGTAGATTGT+AGG + chr8.2:25484084-25484103 MS.gene56797:CDS 45.0%
TGATAGCAAGCAGTTCACCA+CGG + chr8.2:25484339-25484358 MS.gene56797:CDS 45.0%
TTAGACAGCCATAGCATCCA+TGG - chr8.2:25484543-25484562 None:intergenic 45.0%
! GTGTAGACGCCAAGTCATTT+TGG + chr8.2:25483694-25483713 MS.gene56797:CDS 45.0%
!! ATGGAACATGAGGCGTTGTT+AGG - chr8.2:25483456-25483475 None:intergenic 45.0%
!! TAGTGTTTTCAGCTTTGGCG+TGG - chr8.2:25483509-25483528 None:intergenic 45.0%
AACTTCTTTCCACCACTCGC+TGG + chr8.2:25483402-25483421 MS.gene56797:CDS 50.0%
ATTACAAGACGACCAGCGAG+TGG - chr8.2:25483417-25483436 None:intergenic 50.0%
CTACCTGGATCCGCTATAGA+CGG - chr8.2:25484302-25484321 None:intergenic 50.0%
TACCTGGATCCGCTATAGAC+GGG - chr8.2:25484301-25484320 None:intergenic 50.0%
TCTTGGGCAGAAATCGCACA+AGG + chr8.2:25483729-25483748 MS.gene56797:CDS 50.0%
TGGTTGAAGGATGTCGGCAA+TGG - chr8.2:25483532-25483551 None:intergenic 50.0%
ACCTGGATCCGCTATAGACG+GGG - chr8.2:25484300-25484319 None:intergenic 55.0%
ACTTGGCGTCTACACCCACA+TGG - chr8.2:25483689-25483708 None:intergenic 55.0%
GGGAGTGGAATTGGAGCAAC+TGG + chr8.2:25484143-25484162 MS.gene56797:CDS 55.0%
!! GTGCTTGGTGGTGCAGAAGA+AGG + chr8.2:25484452-25484471 MS.gene56797:CDS 55.0%
ACAAGACGACCAGCGAGTGG+TGG - chr8.2:25483414-25483433 None:intergenic 60.0%
!! ACCCCGTCTATAGCGGATCC+AGG + chr8.2:25484296-25484315 MS.gene56797:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 25483201 25484562 25483201 ID=MS.gene56797
chr8.2 mRNA 25483201 25484562 25483201 ID=MS.gene56797.t1;Parent=MS.gene56797
chr8.2 exon 25483201 25484562 25483201 ID=MS.gene56797.t1.exon1;Parent=MS.gene56797.t1
chr8.2 CDS 25483201 25484562 25483201 ID=cds.MS.gene56797.t1;Parent=MS.gene56797.t1
Gene Sequence

>MS.gene56797

ATGGCAGGCATTCTACTTATCTCGACTACTACAATCCGAGCAACAAAACATGGTGATGACAACTCAACTCACAAAATTATCGATTTAGCACCATGGGATCTCAAATATCTTAAAATGGCACCAATCCAAAAAGGTCTTCTTTTCCATAAACCCAAAACAAACCAAATCGACCATCTTAAACAAACACTTTCCAACACTCTCAACTTCTTTCCACCACTCGCTGGTCGTCTTGTAATAACACAACACGACGAACCTAACAACGCCTCATGTTCCATCATTTGCAACAACGCTGGTGCATTGTTTGTCCACGCCAAAGCTGAAAACACTACCATTGCCGACATCCTTCAACCAAAGTATGTTCCTCCCATTGTTTCGTCATTATTCTTACTCAACGAAGTGCAAAACTATGAAGGCACATCACAACCATTACTTGCAGTACAAGTAACAGAGTTAATCGATGGTATCTTCATTGGTTTTGCAATTAACCATGTGGGTGTAGACGCCAAGTCATTTTGGCATTTCATTAACTCTTGGGCAGAAATCGCACAAGGTTTCAATAAGCCAACAAAAATCCCTTCTTTCGAACGTTGGTTTCCAAACTACATTAACCGTCCCATACAGTTTCCTTTCACAAAGGAAGCACAAAAGCAGCCTTTCACAAATGAAGCACAAAATCAACAATGTGAAGTTTCGTCTCATCGAATCTTCCACTTTACTAAGGAACAAATTCTGCAACTCAAATCAAAAGCTAATGCAGAAATTGGTAGTAGTAGTACTAGTAGTGATAAAATAATAACAATATCGTCATTGCAAGCACTTTTAAGTCACCTTTGGCGTTTAATTATAAGTAAACAAAATCTTAAACCAGAAGAAAGGTCTGCTTTCGTGTTGCCTGTAGATTGTAGGACAAGAATGTGTCCAAAGTTAGAAGAGAATTTCTTTGGGAGTGGAATTGGAGCAACTGGATATGTAATAATGCAAGTAGGGGAATTAATGGAAAGTGGGATTGGTAAAATTGCAATGGAAATGAACAAGGAGATTTCATCAAAATCTCATCATGAAAAGGTAGTGAGTAACTATGAGTCTTGGTTGAAAACCCCGTCTATAGCGGATCCAGGTAGATCATCAAGTAATTTGTTGATAGCAAGCAGTTCACCACGGTTCAATTATTACGGTAATGACTTTGGTTGGGGTAAGCCTATTGCAGTTAGAAATGGTGTTGGACTTAAAAGAAACATTGGTAGAGTTATTGTGCTTGGTGGTGCAGAAGAAGGTAGTATTGATATTGAAGTTTGTCTTCCTTATGATATTTTGGAGGCTATGGAAAATGATCATTTGTCCATGGATGCTATGGCTGTCTAA
Protein sequence

>MS.gene56797.t1

MAGILLISTTTIRATKHGDDNSTHKIIDLAPWDLKYLKMAPIQKGLLFHKPKTNQIDHLKQTLSNTLNFFPPLAGRLVITQHDEPNNASCSIICNNAGALFVHAKAENTTIADILQPKYVPPIVSSLFLLNEVQNYEGTSQPLLAVQVTELIDGIFIGFAINHVGVDAKSFWHFINSWAEIAQGFNKPTKIPSFERWFPNYINRPIQFPFTKEAQKQPFTNEAQNQQCEVSSHRIFHFTKEQILQLKSKANAEIGSSSTSSDKIITISSLQALLSHLWRLIISKQNLKPEERSAFVLPVDCRTRMCPKLEENFFGSGIGATGYVIMQVGELMESGIGKIAMEMNKEISSKSHHEKVVSNYESWLKTPSIADPGRSSSNLLIASSSPRFNYYGNDFGWGKPIAVRNGVGLKRNIGRVIVLGGAEEGSIDIEVCLPYDILEAMENDHLSMDAMAV