AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99222

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99222.t1 MTR_4g094835 93.065 447 30 1 1 447 1 446 0.0 864
MS.gene99222.t1 MTR_4g094838 81.556 450 61 5 4 448 1 433 0.0 728
MS.gene99222.t1 MTR_4g094832 86.667 375 46 1 78 448 57 431 0.0 672
MS.gene99222.t1 MTR_4g094842 60.573 454 158 9 1 443 1 444 0.0 541
MS.gene99222.t1 MTR_8g041770 58.705 448 169 7 1 443 1 437 0.0 521
MS.gene99222.t1 MTR_0190s0020 57.684 449 173 8 1 443 1 438 2.86e-179 508
MS.gene99222.t1 MTR_4g022720 56.667 450 169 8 1 443 1 431 5.05e-174 494
MS.gene99222.t1 MTR_8g079710 57.528 445 173 10 1 438 1 436 1.21e-172 491
MS.gene99222.t1 MTR_4g022825 54.945 455 174 9 1 443 1 436 4.77e-171 487
MS.gene99222.t1 MTR_4g094828 54.466 459 184 11 1 445 1 448 6.98e-169 482
MS.gene99222.t1 MTR_3g088420 55.011 449 176 9 1 443 1 429 1.53e-166 476
MS.gene99222.t1 MTR_4g022800 54.923 457 173 9 1 443 1 438 1.98e-166 476
MS.gene99222.t1 MTR_4g022670 53.728 456 179 8 1 443 1 437 2.99e-166 475
MS.gene99222.t1 MTR_4g022290 55.234 449 179 10 1 439 1 437 2.96e-165 473
MS.gene99222.t1 MTR_3g026310 52.916 463 179 9 1 443 1 444 1.97e-163 468
MS.gene99222.t1 MTR_7g026860 52.632 456 181 9 1 443 1 434 1.80e-159 458
MS.gene99222.t1 MTR_4g022760 47.588 456 163 8 1 443 1 393 3.62e-136 397
MS.gene99222.t1 MTR_6g016815 42.188 448 169 13 1 443 15 377 3.44e-103 312
MS.gene99222.t1 MTR_3g101900 42.180 422 227 8 32 443 30 444 1.15e-100 308
MS.gene99222.t1 MTR_3g101930 41.014 434 230 9 28 443 26 451 3.84e-99 304
MS.gene99222.t1 MTR_3g102020 38.961 462 250 10 3 447 5 451 4.67e-97 299
MS.gene99222.t1 MTR_3g102010 41.163 430 231 8 28 443 26 447 2.14e-95 295
MS.gene99222.t1 MTR_3g101960 38.816 456 248 10 3 442 5 445 9.06e-95 293
MS.gene99222.t1 MTR_3g101970 39.344 427 230 8 28 443 25 433 1.93e-93 289
MS.gene99222.t1 MTR_4g094825 75.000 192 46 2 19 210 80 269 1.11e-87 273
MS.gene99222.t1 MTR_4g094825 68.874 151 37 1 298 448 252 392 6.54e-67 219
MS.gene99222.t1 MTR_3g070170 35.870 460 249 8 22 447 23 470 1.08e-84 268
MS.gene99222.t1 MTR_8g040780 58.491 212 81 3 234 442 20 227 2.42e-78 244
MS.gene99222.t1 MTR_5g081960 33.333 483 254 8 22 448 17 487 9.35e-78 250
MS.gene99222.t1 MTR_4g015180 33.735 498 254 10 3 443 5 483 4.74e-77 248
MS.gene99222.t1 MTR_8g040760 53.812 223 91 5 1 220 1 214 2.67e-73 230
MS.gene99222.t1 MTR_7g026850 55.769 156 64 2 37 192 12 162 2.47e-56 184
MS.gene99222.t1 MTR_6g045553 28.908 467 264 12 23 444 29 472 1.65e-49 175
MS.gene99222.t1 MTR_3g102030 32.673 303 170 4 103 401 48 320 6.76e-47 164
MS.gene99222.t1 MTR_6g034485 58.915 129 47 2 199 325 1 125 5.60e-45 153
MS.gene99222.t1 MTR_7g026770 47.222 180 71 4 154 331 132 289 9.68e-43 153
MS.gene99222.t1 MTR_6g034495 58.475 118 48 1 75 192 45 161 2.31e-42 147
MS.gene99222.t1 MTR_7g026830 66.667 81 27 0 97 177 16 96 1.16e-32 119
MS.gene99222.t1 MTR_4g007540 25.570 395 248 14 64 436 56 426 2.85e-30 122
MS.gene99222.t1 MTR_3g098980 28.141 398 226 16 57 430 59 420 4.26e-28 115
MS.gene99222.t1 MTR_8g075610 24.490 392 252 14 64 436 47 413 1.28e-27 114
MS.gene99222.t1 MTR_2g105330 23.192 401 265 13 49 428 39 417 6.20e-26 109
MS.gene99222.t1 MTR_2g064495 23.438 384 253 12 64 428 56 417 6.51e-26 109
MS.gene99222.t1 MTR_8g096530 28.090 356 215 11 57 396 52 382 7.29e-25 106
MS.gene99222.t1 MTR_3g026950 24.818 411 246 13 61 432 51 437 2.53e-23 102
MS.gene99222.t1 MTR_4g097090 23.476 443 281 14 27 428 15 440 9.65e-23 100
MS.gene99222.t1 MTR_3g049150 24.700 417 265 16 50 432 32 433 8.74e-22 97.8
MS.gene99222.t1 MTR_2g089765 27.778 486 261 24 3 431 15 467 1.90e-21 97.1
MS.gene99222.t1 MTR_4g127650 23.009 452 277 15 24 428 13 440 2.19e-21 96.7
MS.gene99222.t1 MTR_8g079970 23.969 388 242 12 49 413 41 398 1.04e-20 94.4
MS.gene99222.t1 MTR_3g086020 26.263 396 245 17 62 448 59 416 1.43e-20 94.0
MS.gene99222.t1 MTR_7g014250 24.444 450 272 18 32 438 26 450 1.44e-20 94.4
MS.gene99222.t1 MTR_7g026800 37.805 164 68 6 223 384 29 160 1.90e-20 89.4
MS.gene99222.t1 MTR_4g055170 28.383 303 190 10 64 355 69 355 2.19e-20 93.6
MS.gene99222.t1 MTR_3g071320 25.797 345 219 12 29 352 23 351 1.36e-19 90.9
MS.gene99222.t1 MTR_7g026780 44.444 108 34 2 336 443 26 107 1.42e-19 84.3
MS.gene99222.t1 MTR_4g022780 36.747 166 71 6 223 386 30 163 1.65e-19 86.3
MS.gene99222.t1 MTR_7g014330 24.163 418 256 16 32 409 26 422 2.02e-19 90.9
MS.gene99222.t1 MTR_7g027175 24.619 394 256 15 57 434 61 429 2.11e-19 90.5
MS.gene99222.t1 MTR_8g012750 25.328 458 270 17 29 434 23 460 2.40e-19 90.5
MS.gene99222.t1 MTR_1g040195 24.941 421 259 15 33 434 23 405 4.51e-19 89.4
MS.gene99222.t1 MTR_1g081660 22.845 464 267 18 32 447 31 451 5.56e-19 89.4
MS.gene99222.t1 MTR_1g053315 25.000 356 222 16 64 397 78 410 1.60e-18 88.2
MS.gene99222.t1 MTR_3g028210 24.411 467 285 18 4 428 8 448 2.73e-18 87.4
MS.gene99222.t1 MTR_4g013050 23.951 405 239 12 57 438 46 404 2.75e-18 87.0
MS.gene99222.t1 MTR_6g015315 24.920 313 202 9 151 437 140 445 5.69e-18 86.3
MS.gene99222.t1 MTR_7g014230 23.923 418 257 16 32 409 28 424 7.40e-18 85.9
MS.gene99222.t1 MTR_6g015850 25.513 439 274 17 30 436 32 449 9.17e-18 85.9
MS.gene99222.t1 MTR_6g015320 24.499 449 260 15 32 434 29 444 2.79e-17 84.3
MS.gene99222.t1 MTR_7g085930 24.831 443 275 16 33 432 20 447 3.11e-17 84.3
MS.gene99222.t1 MTR_2g089670 26.292 445 260 22 30 431 43 462 5.91e-17 83.2
MS.gene99222.t1 MTR_6g015815 24.036 441 281 15 30 436 32 452 1.14e-16 82.4
MS.gene99222.t1 MTR_7g084940 26.039 361 226 14 52 396 57 392 1.16e-16 82.4
MS.gene99222.t1 MTR_1g040175 23.114 411 271 16 48 436 45 432 2.50e-16 81.3
MS.gene99222.t1 MTR_3g087640 26.116 448 274 20 30 434 29 462 5.97e-16 80.1
MS.gene99222.t1 MTR_1g040215 23.077 403 268 13 33 415 23 403 9.97e-16 79.3
MS.gene99222.t1 MTR_5g084850 24.485 388 224 17 49 408 41 387 1.01e-15 79.3
MS.gene99222.t1 MTR_5g084870 23.885 381 235 15 49 408 41 387 1.21e-15 79.0
MS.gene99222.t1 MTR_5g076700 20.635 441 299 13 33 444 24 442 2.40e-15 78.2
MS.gene99222.t1 MTR_7g085990 24.688 401 256 13 64 431 55 442 3.15e-15 77.8
MS.gene99222.t1 MTR_5g026320 23.284 408 257 14 61 442 71 448 3.77e-15 77.8
MS.gene99222.t1 MTR_7g014200 25.721 451 257 20 30 429 28 451 9.85e-15 76.6
MS.gene99222.t1 MTR_7g084910 25.240 313 191 9 40 326 36 331 1.03e-14 76.3
MS.gene99222.t1 MTR_3g049140 24.786 234 154 6 213 432 5 230 1.08e-14 74.3
MS.gene99222.t1 MTR_7g014610 23.758 463 273 19 22 428 19 457 1.17e-14 76.3
MS.gene99222.t1 MTR_7g015860 23.430 414 251 14 58 431 58 445 1.56e-14 75.9
MS.gene99222.t1 MTR_7g114520 21.573 445 289 16 33 443 25 443 1.74e-14 75.5
MS.gene99222.t1 MTR_2g007880 24.257 404 251 17 64 443 74 446 5.43e-14 73.9
MS.gene99222.t1 MTR_7g014160 23.947 451 264 18 32 428 30 455 5.85e-14 73.9
MS.gene99222.t1 MTR_7g114490 22.045 440 273 17 33 434 24 431 6.59e-14 73.9
MS.gene99222.t1 MTR_2g089735 24.887 442 277 18 36 438 30 455 1.44e-13 72.8
MS.gene99222.t1 MTR_3g465980 24.275 276 187 8 64 329 68 331 4.86e-13 71.2
MS.gene99222.t1 MTR_7g114550 20.946 444 293 15 33 444 24 441 7.62e-13 70.5
MS.gene99222.t1 MTR_7g056087 22.330 412 259 17 51 430 41 423 9.43e-13 70.1
MS.gene99222.t1 MTR_1g094790 25.265 471 285 22 4 443 3 437 1.67e-12 69.3
MS.gene99222.t1 MTR_7g014620 24.485 437 275 17 21 428 4 414 1.73e-12 69.3
MS.gene99222.t1 MTR_7g026880 58.000 50 19 1 394 443 2 49 6.36e-12 60.8
MS.gene99222.t1 MTR_7g026870 57.407 54 22 1 303 355 98 151 6.67e-12 63.5
MS.gene99222.t1 MTR_7g013850 22.277 404 236 16 58 406 62 442 7.13e-12 67.8
MS.gene99222.t1 MTR_8g079950 23.438 384 239 14 49 408 41 393 1.38e-11 66.6
MS.gene99222.t1 MTR_2g015430 21.203 349 242 10 64 399 58 386 3.43e-11 65.5
MS.gene99222.t1 MTR_3g465910 25.939 293 181 10 59 334 63 336 5.53e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99222.t1 AT5G67150 41.463 451 244 8 2 443 3 442 2.53e-117 351
MS.gene99222.t1 AT3G50300 41.372 452 243 10 1 441 1 441 9.00e-110 332
MS.gene99222.t1 AT3G50280 39.246 451 254 8 1 443 1 439 3.26e-105 320
MS.gene99222.t1 AT5G67160 38.393 448 246 10 2 442 3 427 5.78e-99 303
MS.gene99222.t1 AT5G38130 39.954 438 239 13 1 429 21 443 1.79e-97 300
MS.gene99222.t1 AT3G50270 37.416 449 261 11 5 443 6 444 2.18e-97 300
MS.gene99222.t1 AT5G42830 37.793 426 241 9 32 443 30 445 1.31e-88 277
MS.gene99222.t1 AT5G07850 38.551 428 238 10 32 443 31 449 1.36e-87 275
MS.gene99222.t1 AT2G39980 34.052 464 242 7 30 443 27 476 3.19e-84 267
MS.gene99222.t1 AT5G07860 38.084 428 236 10 32 443 31 445 8.46e-84 265
MS.gene99222.t1 AT5G01210 35.294 459 246 8 24 443 19 465 6.15e-80 255
MS.gene99222.t1 AT5G07870 36.219 439 241 10 32 443 31 457 2.31e-76 246
MS.gene99222.t1 AT3G50280 40.836 311 177 4 138 443 1 309 3.29e-76 241
MS.gene99222.t1 AT3G50290 41.418 268 147 4 135 394 4 269 7.30e-61 199
MS.gene99222.t1 AT5G23940 32.715 431 266 8 32 444 25 449 1.03e-60 205
MS.gene99222.t1 AT3G29590 27.126 435 283 14 25 438 21 442 4.74e-28 115
MS.gene99222.t1 AT5G48930 24.697 413 257 16 49 434 39 424 1.86e-27 114
MS.gene99222.t1 AT2G19070 25.219 456 279 17 24 436 7 443 6.96e-27 112
MS.gene99222.t1 AT5G41040 25.253 396 243 13 57 430 62 426 2.51e-24 105
MS.gene99222.t1 AT5G41040 25.253 396 243 13 57 430 78 442 2.77e-24 105
MS.gene99222.t1 AT2G40230 24.888 446 288 13 1 423 1 422 5.12e-24 104
MS.gene99222.t1 AT3G29670 24.944 449 297 17 3 428 2 433 1.01e-23 103
MS.gene99222.t1 AT5G39080 24.107 448 279 16 30 436 26 453 8.66e-23 100
MS.gene99222.t1 AT3G29635 23.853 436 273 16 29 428 26 438 2.09e-22 99.8
MS.gene99222.t1 AT3G48720 26.434 401 247 18 57 436 54 427 1.07e-21 97.4
MS.gene99222.t1 AT5G39090 26.744 430 260 21 32 431 28 432 3.77e-21 95.9
MS.gene99222.t1 AT5G57840 25.060 419 266 13 36 428 26 422 1.05e-20 94.4
MS.gene99222.t1 AT1G24430 25.858 379 234 13 55 420 53 397 9.88e-20 91.7
MS.gene99222.t1 AT5G39050 25.915 328 205 14 135 431 132 452 1.27e-19 91.7
MS.gene99222.t1 AT5G07080 28.065 310 177 13 42 327 44 331 2.49e-18 87.4
MS.gene99222.t1 AT5G63560 24.173 393 243 16 57 428 54 412 6.37e-18 85.9
MS.gene99222.t1 AT3G30280 24.242 462 300 20 7 447 4 436 8.47e-18 85.9
MS.gene99222.t1 AT3G03480 23.587 407 260 15 33 416 33 411 1.12e-17 85.5
MS.gene99222.t1 AT5G17540 23.881 402 249 16 33 405 23 396 2.20e-17 84.7
MS.gene99222.t1 AT1G28680 24.355 349 230 13 64 399 62 389 7.11e-17 83.2
MS.gene99222.t1 AT3G26040 23.587 407 252 15 60 443 55 425 1.40e-16 82.0
MS.gene99222.t1 AT1G03940 25.424 413 246 19 58 431 60 449 9.64e-16 79.7
MS.gene99222.t1 AT3G47170 23.197 319 216 8 57 357 63 370 1.20e-15 79.3
MS.gene99222.t1 AT5G61160 24.194 310 202 13 148 434 139 438 4.11e-15 77.8
MS.gene99222.t1 AT1G65450 24.062 320 203 13 55 355 63 361 1.45e-14 75.9
MS.gene99222.t1 AT1G28680 23.746 299 203 9 64 352 62 345 1.58e-14 75.5
MS.gene99222.t1 AT1G24420 24.485 388 244 16 75 447 76 429 3.01e-14 74.7
MS.gene99222.t1 AT1G24420 24.485 388 244 16 75 447 76 429 3.01e-14 74.7
MS.gene99222.t1 AT1G31490 25.000 412 253 16 61 448 59 438 5.45e-14 73.9
MS.gene99222.t1 AT2G25150 22.715 361 237 11 61 400 64 403 2.61e-13 72.0
MS.gene99222.t1 AT5G47950 24.034 466 288 22 1 447 1 419 5.35e-13 70.9
MS.gene99222.t1 AT5G47980 23.245 413 245 17 63 443 60 432 1.17e-12 70.1
MS.gene99222.t1 AT1G27620 21.705 387 266 11 64 436 58 421 2.42e-12 68.9
MS.gene99222.t1 AT1G32910 21.763 363 227 10 64 397 65 399 8.77e-12 67.4
MS.gene99222.t1 AT2G23510 22.767 347 242 12 64 399 69 400 1.81e-11 66.2

Find 87 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGCTTGACGAGGATTATTT+TGG 0.141275 8.4:+26224160 MS.gene99222:CDS
AAGGAACATAGTTTGGTTTA+AGG 0.151696 8.4:-26223613 None:intergenic
TCTCCGAGGTTTGATGTTTA+TGG 0.199950 8.4:+26224388 MS.gene99222:CDS
GTAAAATGGAAAATTCTTTC+AGG 0.213962 8.4:-26223944 None:intergenic
CACATTCTGATGAGAAAATA+AGG 0.214956 8.4:+26224281 MS.gene99222:CDS
CGTTGAAATGAAGGGAGTTT+TGG 0.257934 8.4:-26223836 None:intergenic
GATTTGAACATTCTCTGTTT+TGG 0.269213 8.4:-26224011 None:intergenic
TGGTTTCAACTAGGAGAAAT+TGG 0.291929 8.4:-26223358 None:intergenic
TCAAATGGTTGATTTGGTTT+TGG 0.303035 8.4:-26223418 None:intergenic
GATGTTTATGGTAATGATTT+TGG 0.307637 8.4:+26224400 MS.gene99222:CDS
GCCTGTTAAGGGTGGAAAAT+AGG 0.317928 8.4:-26223465 None:intergenic
GTTGTTTGGGATTAATAGTT+TGG 0.327840 8.4:+26224336 MS.gene99222:CDS
ACGTAACGAAAATCTTCTTC+TGG 0.337895 8.4:-26224103 None:intergenic
ACTATAGAAGGAACATAGTT+TGG 0.352065 8.4:-26223620 None:intergenic
TTTGTTTCAAATGGTTGATT+TGG 0.358387 8.4:-26223424 None:intergenic
TGATGTGTTGTTGTTGTTTG+TGG 0.358976 8.4:-26223516 None:intergenic
GATTATAAGACGGCCTGTTA+AGG 0.359476 8.4:-26223477 None:intergenic
ATTGGAAGTTCAACATCGTT+TGG 0.360160 8.4:-26223866 None:intergenic
TTTGAGATATTGGAGGCTTT+GGG 0.368507 8.4:+26224535 MS.gene99222:CDS
GGGAAGATTACTGTGTTTGC+AGG 0.373043 8.4:+26224472 MS.gene99222:CDS
TTTGTGATGGCAAGATGTTT+TGG 0.386713 8.4:+26223765 MS.gene99222:CDS
TTGAGAATGCCGAAGTTGTT+TGG 0.387603 8.4:+26224322 MS.gene99222:CDS
GGGAAATGATGCTGAGTTCA+TGG 0.395255 8.4:+26224555 MS.gene99222:CDS
TTAGCTAATGGCATCTTCAT+TGG 0.396826 8.4:+26223722 MS.gene99222:CDS
TTGGAAGTTCAACATCGTTT+GGG 0.400372 8.4:-26223865 None:intergenic
CATTAGCTAACTCCGTTACT+TGG 0.402418 8.4:-26223709 None:intergenic
TGGAAAGTGTTTGTTTCAAA+TGG 0.404356 8.4:-26223433 None:intergenic
TTTGGTTTGGAATGGGTTGG+TGG 0.407717 8.4:-26223400 None:intergenic
TTTGGAAATGCAGGGATCAT+TGG 0.408778 8.4:+26224178 MS.gene99222:CDS
TGAGAATGCCGAAGTTGTTT+GGG 0.411706 8.4:+26224323 MS.gene99222:CDS
TTTGGCAGGTGCTAATTCAT+TGG 0.414867 8.4:+26224354 MS.gene99222:CDS
CTAGTTGAAACCATCCAAAA+AGG 0.430223 8.4:+26223368 MS.gene99222:CDS
TTTGGCATTTCGTCAATACT+TGG 0.430278 8.4:+26223783 MS.gene99222:CDS
TTTGTGAAAGGAAATTGTAT+TGG 0.434338 8.4:-26223884 None:intergenic
TTTGGGATTAATAGTTTGGC+AGG 0.440696 8.4:+26224340 MS.gene99222:CDS
TGGGATTGGAAAATGTTCAT+TGG 0.444512 8.4:+26224237 MS.gene99222:CDS
AAGCGTTGTTCTCTCATGTA+TGG 0.446035 8.4:+26224050 MS.gene99222:CDS
GTAGTGTAACTATGAAAGTA+GGG 0.457732 8.4:+26224200 MS.gene99222:CDS
TTGAGATTATTGTTGTCAAT+TGG 0.459438 8.4:-26223290 None:intergenic
GGTAGTGTAACTATGAAAGT+AGG 0.460447 8.4:+26224199 MS.gene99222:CDS
GGAGTGCAAATAAAAGTAAT+GGG 0.465217 8.4:+26224452 MS.gene99222:CDS
CTCCCACTTCGAACCCCTAC+CGG 0.466626 8.4:-26224433 None:intergenic
GAATGGGTTGGTGGAAAAGA+AGG 0.467476 8.4:-26223391 None:intergenic
ATTATAAGACGGCCTGTTAA+GGG 0.469230 8.4:-26223476 None:intergenic
GGGAACCAACGTTGAAATGA+AGG 0.474068 8.4:-26223845 None:intergenic
GGGAGTGCAAATAAAAGTAA+TGG 0.474744 8.4:+26224451 MS.gene99222:CDS
GTTTGTGGCTACGTTGTGTT+GGG 0.476915 8.4:-26223501 None:intergenic
AAACCGGTAGGGGTTCGAAG+TGG 0.479206 8.4:+26224430 MS.gene99222:CDS
CAAGTAACGGAGTTAGCTAA+TGG 0.482336 8.4:+26223710 MS.gene99222:CDS
ACCAAGGTTGTTGCAAATGA+TGG 0.487314 8.4:-26223543 None:intergenic
CAGTCACATTACTTAGAACT+AGG 0.487464 8.4:-26224580 None:intergenic
GTTACTTGGACTGCTAGAAT+AGG 0.498322 8.4:-26223695 None:intergenic
TGTTTGTGGCTACGTTGTGT+TGG 0.512319 8.4:-26223502 None:intergenic
CTCAACCACGTTGTTTGTGA+TGG 0.514978 8.4:+26223752 MS.gene99222:CDS
GAGATTCTAGAATGTGGGAT+TGG 0.518222 8.4:+26224223 MS.gene99222:CDS
TAGGGGAGATTCTAGAATGT+GGG 0.520801 8.4:+26224218 MS.gene99222:CDS
TCCATCATTTGCAACAACCT+TGG 0.522468 8.4:+26223542 MS.gene99222:CDS
TATTGTTGTCAATTGGTGCT+TGG 0.527153 8.4:-26223283 None:intergenic
GTAGGGGAGATTCTAGAATG+TGG 0.529927 8.4:+26224217 MS.gene99222:CDS
ACATTCTGATGAGAAAATAA+GGG 0.533534 8.4:+26224282 MS.gene99222:CDS
AACCGGTAGGGGTTCGAAGT+GGG 0.542449 8.4:+26224431 MS.gene99222:CDS
TCAAGCGGAGGAATCATCCT+TGG 0.542679 8.4:-26224145 None:intergenic
AACTCCCTTCATTTCAACGT+TGG 0.545872 8.4:+26223840 MS.gene99222:CDS
CTTTCAGGTAAGTTCTGCGT+CGG 0.565322 8.4:-26223929 None:intergenic
AATAATCGATTTAACACCAT+GGG 0.569717 8.4:+26223331 MS.gene99222:CDS
GGAACCAACGTTGAAATGAA+GGG 0.576282 8.4:-26223844 None:intergenic
GTGTTTGCAGGAGCTGAAGA+AGG 0.577173 8.4:+26224484 MS.gene99222:CDS
CTATTAATCCCAAACAACTT+CGG 0.578183 8.4:-26224331 None:intergenic
TCTCTTCGTCCACGCAAAAG+CGG 0.578199 8.4:+26223568 MS.gene99222:CDS
CCAAAATAATCCTCGTCAAG+CGG 0.581464 8.4:-26224160 None:intergenic
TAAGGGATGATTATGAGTGT+TGG 0.592031 8.4:+26224299 MS.gene99222:CDS
TAGTTATAGGTGTTAGACCA+AGG 0.600032 8.4:+26224128 MS.gene99222:CDS
AGGAGAAATTGGAGATCCCA+TGG 0.609549 8.4:-26223347 None:intergenic
AAATAATCGATTTAACACCA+TGG 0.626064 8.4:+26223330 MS.gene99222:CDS
TATTCTAGCAGTCCAAGTAA+CGG 0.629197 8.4:+26223697 MS.gene99222:CDS
TGTGTTGGGTGATTATAAGA+CGG 0.629490 8.4:-26223487 None:intergenic
GATGATTCCTCCGCTTGACG+AGG 0.630798 8.4:+26224150 MS.gene99222:CDS
AAATAATCCTCGTCAAGCGG+AGG 0.636490 8.4:-26224157 None:intergenic
TGCTAGAATAGGTTGTGAAG+TGG 0.647856 8.4:-26223684 None:intergenic
ATAAGACGGCCTGTTAAGGG+TGG 0.648919 8.4:-26223473 None:intergenic
ACTAGTTGAGATCACTGTGA+TGG 0.656954 8.4:-26223255 None:intergenic
TGGAAAATAGGAGAGTGTTG+TGG 0.662672 8.4:-26223453 None:intergenic
TAGTGTAACTATGAAAGTAG+GGG 0.674189 8.4:+26224201 MS.gene99222:CDS
TTACCATAAACATCAAACCT+CGG 0.674952 8.4:-26224391 None:intergenic
TGGCTACGAGTAGTTCTCCG+AGG 0.676435 8.4:+26224374 MS.gene99222:CDS
CGTGGACGAAGAGAGCACCA+AGG 0.726493 8.4:-26223559 None:intergenic
TCTTGCCATCACAAACAACG+TGG 0.787958 8.4:-26223757 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAATTTTCCATTTTACAA+AGG + chr8.4:26223949-26223968 MS.gene99222:CDS 20.0%
! AAAAATGAGTGAACTATAGA+AGG - chr8.4:26223635-26223654 None:intergenic 25.0%
! ACATTCTGATGAGAAAATAA+GGG + chr8.4:26224282-26224301 MS.gene99222:CDS 25.0%
! GTAAAATGGAAAATTCTTTC+AGG - chr8.4:26223947-26223966 None:intergenic 25.0%
! TTGAGATTATTGTTGTCAAT+TGG - chr8.4:26223293-26223312 None:intergenic 25.0%
! TTTGTGAAAGGAAATTGTAT+TGG - chr8.4:26223887-26223906 None:intergenic 25.0%
! TTTGTTTCAAATGGTTGATT+TGG - chr8.4:26223427-26223446 None:intergenic 25.0%
!! AAATAATCGATTTAACACCA+TGG + chr8.4:26223330-26223349 MS.gene99222:CDS 25.0%
!! AATAATCGATTTAACACCAT+GGG + chr8.4:26223331-26223350 MS.gene99222:CDS 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr8.4:26224400-26224419 MS.gene99222:CDS 25.0%
!!! TTATGGTAATGATTTTGGTT+GGG + chr8.4:26224405-26224424 MS.gene99222:CDS 25.0%
!!! TTGTCTTTCTTTTGAGATAT+TGG + chr8.4:26224525-26224544 MS.gene99222:CDS 25.0%
!!! TTTATGGTAATGATTTTGGT+TGG + chr8.4:26224404-26224423 MS.gene99222:CDS 25.0%
!!! TTTCGTTACGTTTTAGTTAT+AGG + chr8.4:26224115-26224134 MS.gene99222:CDS 25.0%
ACTATAGAAGGAACATAGTT+TGG - chr8.4:26223623-26223642 None:intergenic 30.0%
CACATTCTGATGAGAAAATA+AGG + chr8.4:26224281-26224300 MS.gene99222:CDS 30.0%
CTATTAATCCCAAACAACTT+CGG - chr8.4:26224334-26224353 None:intergenic 30.0%
GGAGTGCAAATAAAAGTAAT+GGG + chr8.4:26224452-26224471 MS.gene99222:CDS 30.0%
GTAGTGTAACTATGAAAGTA+GGG + chr8.4:26224200-26224219 MS.gene99222:CDS 30.0%
TAGTGTAACTATGAAAGTAG+GGG + chr8.4:26224201-26224220 MS.gene99222:CDS 30.0%
TTACCATAAACATCAAACCT+CGG - chr8.4:26224394-26224413 None:intergenic 30.0%
! AAGGAACATAGTTTGGTTTA+AGG - chr8.4:26223616-26223635 None:intergenic 30.0%
! CGATTTTCTCCTTTGTAAAA+TGG - chr8.4:26223961-26223980 None:intergenic 30.0%
! GATTTGAACATTCTCTGTTT+TGG - chr8.4:26224014-26224033 None:intergenic 30.0%
! GTTGTTTGGGATTAATAGTT+TGG + chr8.4:26224336-26224355 MS.gene99222:CDS 30.0%
! TTTTACAAAGGAGAAAATCG+CGG + chr8.4:26223961-26223980 MS.gene99222:CDS 30.0%
!! AGTTTTTAACTCCGTTAAGT+GGG - chr8.4:26223658-26223677 None:intergenic 30.0%
!! TAGTTTTTAACTCCGTTAAG+TGG - chr8.4:26223659-26223678 None:intergenic 30.0%
!! TGGAAAGTGTTTGTTTCAAA+TGG - chr8.4:26223436-26223455 None:intergenic 30.0%
!!! AGGATTATTTTGGAAATGCA+GGG + chr8.4:26224170-26224189 MS.gene99222:CDS 30.0%
!!! ATTTGGTTTTGGTTTGGAAT+GGG - chr8.4:26223410-26223429 None:intergenic 30.0%
!!! TATGGTAATGATTTTGGTTG+GGG + chr8.4:26224406-26224425 MS.gene99222:CDS 30.0%
!!! TCAAATGGTTGATTTGGTTT+TGG - chr8.4:26223421-26223440 None:intergenic 30.0%
!!! TCTTTCTTTTGAGATATTGG+AGG + chr8.4:26224528-26224547 MS.gene99222:CDS 30.0%
ACGTAACGAAAATCTTCTTC+TGG - chr8.4:26224106-26224125 None:intergenic 35.0%
ATTATAAGACGGCCTGTTAA+GGG - chr8.4:26223479-26223498 None:intergenic 35.0%
ATTGGAAGTTCAACATCGTT+TGG - chr8.4:26223869-26223888 None:intergenic 35.0%
CTAGTTGAAACCATCCAAAA+AGG + chr8.4:26223368-26223387 MS.gene99222:CDS 35.0%
GGTAGTGTAACTATGAAAGT+AGG + chr8.4:26224199-26224218 MS.gene99222:CDS 35.0%
TAAGGGATGATTATGAGTGT+TGG + chr8.4:26224299-26224318 MS.gene99222:CDS 35.0%
TAGTTATAGGTGTTAGACCA+AGG + chr8.4:26224128-26224147 MS.gene99222:CDS 35.0%
TATTCTAGCAGTCCAAGTAA+CGG + chr8.4:26223697-26223716 MS.gene99222:CDS 35.0%
TGGGATTGGAAAATGTTCAT+TGG + chr8.4:26224237-26224256 MS.gene99222:CDS 35.0%
TGGTTTCAACTAGGAGAAAT+TGG - chr8.4:26223361-26223380 None:intergenic 35.0%
TGTGTTGGGTGATTATAAGA+CGG - chr8.4:26223490-26223509 None:intergenic 35.0%
TTAGCTAATGGCATCTTCAT+TGG + chr8.4:26223722-26223741 MS.gene99222:CDS 35.0%
TTGGAAGTTCAACATCGTTT+GGG - chr8.4:26223868-26223887 None:intergenic 35.0%
TTTGGCATTTCGTCAATACT+TGG + chr8.4:26223783-26223802 MS.gene99222:CDS 35.0%
! CGGTTTTCTACATTGTGATT+CGG - chr8.4:26223912-26223931 None:intergenic 35.0%
! CTTTTTGGATGGTTTCAACT+AGG - chr8.4:26223370-26223389 None:intergenic 35.0%
! GGGAGTGCAAATAAAAGTAA+TGG + chr8.4:26224451-26224470 MS.gene99222:CDS 35.0%
! TATTGTTGTCAATTGGTGCT+TGG - chr8.4:26223286-26223305 None:intergenic 35.0%
! TTTGAGATATTGGAGGCTTT+GGG + chr8.4:26224535-26224554 MS.gene99222:CDS 35.0%
! TTTGGGATTAATAGTTTGGC+AGG + chr8.4:26224340-26224359 MS.gene99222:CDS 35.0%
! TTTGTGATGGCAAGATGTTT+TGG + chr8.4:26223765-26223784 MS.gene99222:CDS 35.0%
!! CACTCATTTTTCCCACTTAA+CGG + chr8.4:26223644-26223663 MS.gene99222:CDS 35.0%
!! TGATGTGTTGTTGTTGTTTG+TGG - chr8.4:26223519-26223538 None:intergenic 35.0%
!!! ATGGTAATGATTTTGGTTGG+GGG + chr8.4:26224407-26224426 MS.gene99222:CDS 35.0%
!!! GAGGATTATTTTGGAAATGC+AGG + chr8.4:26224169-26224188 MS.gene99222:CDS 35.0%
!!! GATTTGGTTTTGGTTTGGAA+TGG - chr8.4:26223411-26223430 None:intergenic 35.0%
!!! TGGTTGATTTGGTTTTGGTT+TGG - chr8.4:26223416-26223435 None:intergenic 35.0%
!!! TGTGATTCGGTTTTTGTGAA+AGG - chr8.4:26223899-26223918 None:intergenic 35.0%
!!! TTTTGAGATATTGGAGGCTT+TGG + chr8.4:26224534-26224553 MS.gene99222:CDS 35.0%
AACTCCCTTCATTTCAACGT+TGG + chr8.4:26223840-26223859 MS.gene99222:CDS 40.0%
ACCAAGGTTGTTGCAAATGA+TGG - chr8.4:26223546-26223565 None:intergenic 40.0%
ACTAGTTGAGATCACTGTGA+TGG - chr8.4:26223258-26223277 None:intergenic 40.0%
CATTAGCTAACTCCGTTACT+TGG - chr8.4:26223712-26223731 None:intergenic 40.0%
CCAAAATAATCCTCGTCAAG+CGG - chr8.4:26224163-26224182 None:intergenic 40.0%
GATTATAAGACGGCCTGTTA+AGG - chr8.4:26223480-26223499 None:intergenic 40.0%
GGAACCAACGTTGAAATGAA+GGG - chr8.4:26223847-26223866 None:intergenic 40.0%
GTTACTTGGACTGCTAGAAT+AGG - chr8.4:26223698-26223717 None:intergenic 40.0%
TCCATCATTTGCAACAACCT+TGG + chr8.4:26223542-26223561 MS.gene99222:CDS 40.0%
TCTCCGAGGTTTGATGTTTA+TGG + chr8.4:26224388-26224407 MS.gene99222:CDS 40.0%
TGAGAATGCCGAAGTTGTTT+GGG + chr8.4:26224323-26224342 MS.gene99222:CDS 40.0%
TGCTAGAATAGGTTGTGAAG+TGG - chr8.4:26223687-26223706 None:intergenic 40.0%
TTGAGAATGCCGAAGTTGTT+TGG + chr8.4:26224322-26224341 MS.gene99222:CDS 40.0%
TTTGGAAATGCAGGGATCAT+TGG + chr8.4:26224178-26224197 MS.gene99222:CDS 40.0%
TTTGGCAGGTGCTAATTCAT+TGG + chr8.4:26224354-26224373 MS.gene99222:CDS 40.0%
! AAGCGTTGTTCTCTCATGTA+TGG + chr8.4:26224050-26224069 MS.gene99222:CDS 40.0%
! CAAGTAACGGAGTTAGCTAA+TGG + chr8.4:26223710-26223729 MS.gene99222:CDS 40.0%
! CGTTGAAATGAAGGGAGTTT+TGG - chr8.4:26223839-26223858 None:intergenic 40.0%
! GAAAAGAAGGCCTTTTTGGA+TGG - chr8.4:26223381-26223400 None:intergenic 40.0%
! TCCTATTTTCCACCCTTAAC+AGG + chr8.4:26223464-26223483 MS.gene99222:CDS 40.0%
!! AAGGGAGTTTTGGTATACTC+TGG - chr8.4:26223829-26223848 None:intergenic 40.0%
!! AGGGAGTTTTGGTATACTCT+GGG - chr8.4:26223828-26223847 None:intergenic 40.0%
!! GAGATTCTAGAATGTGGGAT+TGG + chr8.4:26224223-26224242 MS.gene99222:CDS 40.0%
!! TAGGGGAGATTCTAGAATGT+GGG + chr8.4:26224218-26224237 MS.gene99222:CDS 40.0%
!! TGGAAAATAGGAGAGTGTTG+TGG - chr8.4:26223456-26223475 None:intergenic 40.0%
AAATAATCCTCGTCAAGCGG+AGG - chr8.4:26224160-26224179 None:intergenic 45.0%
AGGAGAAATTGGAGATCCCA+TGG - chr8.4:26223350-26223369 None:intergenic 45.0%
CTCAACCACGTTGTTTGTGA+TGG + chr8.4:26223752-26223771 MS.gene99222:CDS 45.0%
GCCTGTTAAGGGTGGAAAAT+AGG - chr8.4:26223468-26223487 None:intergenic 45.0%
GGGAAATGATGCTGAGTTCA+TGG + chr8.4:26224555-26224574 MS.gene99222:CDS 45.0%
GGGAACCAACGTTGAAATGA+AGG - chr8.4:26223848-26223867 None:intergenic 45.0%
GGGAAGATTACTGTGTTTGC+AGG + chr8.4:26224472-26224491 MS.gene99222:CDS 45.0%
GTTTGTGGCTACGTTGTGTT+GGG - chr8.4:26223504-26223523 None:intergenic 45.0%
TCTTGCCATCACAAACAACG+TGG - chr8.4:26223760-26223779 None:intergenic 45.0%
TGTTTGTGGCTACGTTGTGT+TGG - chr8.4:26223505-26223524 None:intergenic 45.0%
! CCGCTTGACGAGGATTATTT+TGG + chr8.4:26224160-26224179 MS.gene99222:CDS 45.0%
! CTTTCAGGTAAGTTCTGCGT+CGG - chr8.4:26223932-26223951 None:intergenic 45.0%
! GGTGGAAAAGAAGGCCTTTT+TGG - chr8.4:26223385-26223404 None:intergenic 45.0%
!! GAATGGGTTGGTGGAAAAGA+AGG - chr8.4:26223394-26223413 None:intergenic 45.0%
!! GTAGGGGAGATTCTAGAATG+TGG + chr8.4:26224217-26224236 MS.gene99222:CDS 45.0%
!! TTGTGTTTTCCGCTTTTGCG+TGG - chr8.4:26223580-26223599 None:intergenic 45.0%
!! TTTGGTTTGGAATGGGTTGG+TGG - chr8.4:26223403-26223422 None:intergenic 45.0%
!!! GGTTTTGGTTTGGAATGGGT+TGG - chr8.4:26223406-26223425 None:intergenic 45.0%
!!! TGATTTTGGTTGGGGGAAAC+CGG + chr8.4:26224414-26224433 MS.gene99222:CDS 45.0%
ATAAGACGGCCTGTTAAGGG+TGG - chr8.4:26223476-26223495 None:intergenic 50.0%
GTGTTTGCAGGAGCTGAAGA+AGG + chr8.4:26224484-26224503 MS.gene99222:CDS 50.0%
TCAAGCGGAGGAATCATCCT+TGG - chr8.4:26224148-26224167 None:intergenic 50.0%
TCTCTTCGTCCACGCAAAAG+CGG + chr8.4:26223568-26223587 MS.gene99222:CDS 50.0%
AAACCGGTAGGGGTTCGAAG+TGG + chr8.4:26224430-26224449 MS.gene99222:CDS 55.0%
AACCGGTAGGGGTTCGAAGT+GGG + chr8.4:26224431-26224450 MS.gene99222:CDS 55.0%
GATGATTCCTCCGCTTGACG+AGG + chr8.4:26224150-26224169 MS.gene99222:CDS 55.0%
TGGCTACGAGTAGTTCTCCG+AGG + chr8.4:26224374-26224393 MS.gene99222:CDS 55.0%
TTGGTTGGGGGAAACCGGTA+GGG + chr8.4:26224419-26224438 MS.gene99222:CDS 55.0%
TTTGGTTGGGGGAAACCGGT+AGG + chr8.4:26224418-26224437 MS.gene99222:CDS 55.0%
CTCCCACTTCGAACCCCTAC+CGG - chr8.4:26224436-26224455 None:intergenic 60.0%
TGGTTGGGGGAAACCGGTAG+GGG + chr8.4:26224420-26224439 MS.gene99222:CDS 60.0%
!! CGTGGACGAAGAGAGCACCA+AGG - chr8.4:26223562-26223581 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 26223248 26224594 26223248 ID=MS.gene99222
chr8.4 mRNA 26223248 26224594 26223248 ID=MS.gene99222.t1;Parent=MS.gene99222
chr8.4 exon 26223248 26224594 26223248 ID=MS.gene99222.t1.exon1;Parent=MS.gene99222.t1
chr8.4 CDS 26223248 26224594 26223248 ID=cds.MS.gene99222.t1;Parent=MS.gene99222.t1
Gene Sequence

>MS.gene99222

ATGGATTCCATCACAGTGATCTCAACTAGTACAATCCAAGCACCAATTGACAACAATAATCTCAACTCAAGAAGTGATTATCAAATAATCGATTTAACACCATGGGATCTCCAATTTCTCCTAGTTGAAACCATCCAAAAAGGCCTTCTTTTCCACCAACCCATTCCAAACCAAAACCAAATCAACCATTTGAAACAAACACTTTCCACAACACTCTCCTATTTTCCACCCTTAACAGGCCGTCTTATAATCACCCAACACAACGTAGCCACAAACAACAACAACACATCATGTTCCATCATTTGCAACAACCTTGGTGCTCTCTTCGTCCACGCAAAAGCGGAAAACACAACTGTATCAGACATCCTTAAACCAAACTATGTTCCTTCTATAGTTCACTCATTTTTCCCACTTAACGGAGTTAAAAACTACGAAGCCACTTCACAACCTATTCTAGCAGTCCAAGTAACGGAGTTAGCTAATGGCATCTTCATTGGTTTGACTCTCAACCACGTTGTTTGTGATGGCAAGATGTTTTGGCATTTCGTCAATACTTGGTCACAAATCTCAAAAAACTCCCAGAGTATACCAAAACTCCCTTCATTTCAACGTTGGTTCCCAAACGATGTTGAACTTCCAATACAATTTCCTTTCACAAAAACCGAATCACAATGTAGAAAACCGACGCAGAACTTACCTGAAAGAATTTTCCATTTTACAAAGGAGAAAATCGCGGAACTGAAATCAAAAGCAAATGAAGAAACCAAAACAGAGAATGTTCAAATCAAAATATCTTCGCTTCAAGCGTTGTTCTCTCATGTATGGCGATGTGTTGTTCGAAGCAAAAAACTCGATCCAGAAGAAGATTTTCGTTACGTTTTAGTTATAGGTGTTAGACCAAGGATGATTCCTCCGCTTGACGAGGATTATTTTGGAAATGCAGGGATCATTGGTAGTGTAACTATGAAAGTAGGGGAGATTCTAGAATGTGGGATTGGAAAATGTTCATTGGAGATGAACAAGATGATTATGTCACATTCTGATGAGAAAATAAGGGATGATTATGAGTGTTGGTTGAGAATGCCGAAGTTGTTTGGGATTAATAGTTTGGCAGGTGCTAATTCATTGGCTACGAGTAGTTCTCCGAGGTTTGATGTTTATGGTAATGATTTTGGTTGGGGGAAACCGGTAGGGGTTCGAAGTGGGAGTGCAAATAAAAGTAATGGGAAGATTACTGTGTTTGCAGGAGCTGAAGAAGGTAGCATTGATATTGAAGTTTGTCTTTCTTTTGAGATATTGGAGGCTTTGGGAAATGATGCTGAGTTCATGGTTCCTAGTTCTAAGTAA
Protein sequence

>MS.gene99222.t1

MDSITVISTSTIQAPIDNNNLNSRSDYQIIDLTPWDLQFLLVETIQKGLLFHQPIPNQNQINHLKQTLSTTLSYFPPLTGRLIITQHNVATNNNNTSCSIICNNLGALFVHAKAENTTVSDILKPNYVPSIVHSFFPLNGVKNYEATSQPILAVQVTELANGIFIGLTLNHVVCDGKMFWHFVNTWSQISKNSQSIPKLPSFQRWFPNDVELPIQFPFTKTESQCRKPTQNLPERIFHFTKEKIAELKSKANEETKTENVQIKISSLQALFSHVWRCVVRSKKLDPEEDFRYVLVIGVRPRMIPPLDEDYFGNAGIIGSVTMKVGEILECGIGKCSLEMNKMIMSHSDEKIRDDYECWLRMPKLFGINSLAGANSLATSSSPRFDVYGNDFGWGKPVGVRSGSANKSNGKITVFAGAEEGSIDIEVCLSFEILEALGNDAEFMVPSSK