AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87536

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87536.t1 MTR_4g022800 88.262 443 51 1 1 442 1 443 0.0 813
MS.gene87536.t1 MTR_4g022720 87.557 442 49 1 1 442 1 436 0.0 804
MS.gene87536.t1 MTR_7g026860 86.878 442 55 1 1 442 1 439 0.0 801
MS.gene87536.t1 MTR_4g022670 83.258 442 74 0 1 442 1 442 0.0 773
MS.gene87536.t1 MTR_4g022825 75.057 441 109 1 1 441 1 440 0.0 692
MS.gene87536.t1 MTR_4g022760 75.792 442 63 1 1 442 1 398 0.0 677
MS.gene87536.t1 MTR_3g088420 68.552 442 131 2 1 442 1 434 0.0 619
MS.gene87536.t1 MTR_3g026310 66.815 449 142 2 1 442 1 449 0.0 611
MS.gene87536.t1 MTR_0190s0020 58.186 452 168 7 1 441 1 442 0.0 528
MS.gene87536.t1 MTR_8g041770 59.161 453 163 6 1 442 1 442 0.0 527
MS.gene87536.t1 MTR_4g094842 56.893 457 168 10 1 439 1 446 8.06e-171 487
MS.gene87536.t1 MTR_4g094835 52.308 455 186 8 1 437 1 442 1.57e-161 463
MS.gene87536.t1 MTR_6g016815 52.715 442 135 7 1 442 15 382 1.23e-147 425
MS.gene87536.t1 MTR_4g094828 50.647 464 192 15 1 441 1 450 6.88e-146 424
MS.gene87536.t1 MTR_4g094838 49.453 457 179 9 4 437 1 428 1.36e-145 422
MS.gene87536.t1 MTR_4g022290 49.892 461 191 13 1 439 1 443 5.53e-145 421
MS.gene87536.t1 MTR_8g079710 50.331 453 187 11 1 432 1 436 2.80e-144 419
MS.gene87536.t1 MTR_4g094832 54.830 383 146 6 69 437 57 426 3.48e-138 403
MS.gene87536.t1 MTR_3g101900 40.503 437 235 12 18 441 24 448 2.51e-97 299
MS.gene87536.t1 MTR_3g101930 40.449 445 233 11 16 440 22 454 4.06e-96 296
MS.gene87536.t1 MTR_7g026850 79.042 167 35 0 29 195 12 178 6.14e-94 281
MS.gene87536.t1 MTR_3g102020 39.367 442 241 10 16 441 21 451 3.33e-92 286
MS.gene87536.t1 MTR_7g026770 69.697 198 32 1 141 338 132 301 1.83e-90 276
MS.gene87536.t1 MTR_3g101960 39.359 437 237 10 16 436 21 445 1.22e-89 280
MS.gene87536.t1 MTR_8g040760 63.850 213 70 3 1 207 1 212 9.67e-89 269
MS.gene87536.t1 MTR_3g101970 38.182 440 233 10 16 440 21 436 8.35e-88 275
MS.gene87536.t1 MTR_3g102010 38.688 442 243 12 16 441 22 451 1.07e-87 275
MS.gene87536.t1 MTR_8g040780 58.525 217 86 2 224 436 11 227 1.54e-79 246
MS.gene87536.t1 MTR_4g015180 34.151 489 241 15 16 442 19 488 3.90e-63 212
MS.gene87536.t1 MTR_5g081960 31.789 475 268 11 16 441 19 486 7.94e-61 206
MS.gene87536.t1 MTR_3g070170 30.968 465 263 10 16 441 25 470 7.41e-60 202
MS.gene87536.t1 MTR_4g094825 50.794 189 84 5 17 199 84 269 5.82e-53 182
MS.gene87536.t1 MTR_4g094825 48.734 158 68 2 286 441 245 391 9.78e-43 155
MS.gene87536.t1 MTR_3g102030 34.545 330 174 7 73 397 28 320 9.25e-50 172
MS.gene87536.t1 MTR_6g034495 57.419 155 57 3 33 179 8 161 3.13e-49 165
MS.gene87536.t1 MTR_6g034485 62.121 132 42 2 189 320 2 125 2.29e-46 156
MS.gene87536.t1 MTR_7g026800 51.497 167 51 2 212 377 19 156 1.57e-45 157
MS.gene87536.t1 MTR_4g022780 49.724 181 58 4 200 377 7 157 2.87e-45 155
MS.gene87536.t1 MTR_6g045553 28.541 473 284 13 4 437 14 471 5.04e-45 163
MS.gene87536.t1 MTR_7g026830 83.529 85 14 0 82 166 14 98 1.92e-44 150
MS.gene87536.t1 MTR_7g026780 65.766 111 14 2 332 442 26 112 1.15e-37 133
MS.gene87536.t1 MTR_4g007540 26.292 445 272 17 16 432 12 428 3.88e-31 124
MS.gene87536.t1 MTR_8g075610 26.209 393 249 12 55 431 47 414 6.83e-29 117
MS.gene87536.t1 MTR_2g105330 26.768 396 246 13 55 431 56 426 1.30e-28 117
MS.gene87536.t1 MTR_2g064495 26.343 391 254 12 55 431 56 426 1.78e-28 117
MS.gene87536.t1 MTR_8g096530 27.665 394 251 11 53 435 57 427 2.79e-27 113
MS.gene87536.t1 MTR_7g026870 88.889 54 6 0 298 351 98 151 5.26e-27 106
MS.gene87536.t1 MTR_4g013050 27.824 363 190 12 53 393 51 363 6.69e-24 103
MS.gene87536.t1 MTR_3g098980 26.515 396 240 15 55 431 66 429 1.71e-23 102
MS.gene87536.t1 MTR_3g026950 26.316 418 234 18 52 426 51 437 2.98e-23 102
MS.gene87536.t1 MTR_3g049150 26.509 381 247 13 41 399 37 406 1.52e-20 94.0
MS.gene87536.t1 MTR_1g081660 23.214 392 253 15 24 393 31 396 3.54e-19 89.7
MS.gene87536.t1 MTR_7g026880 67.308 52 17 0 390 441 2 53 4.53e-19 80.9
MS.gene87536.t1 MTR_2g089765 23.690 439 267 15 3 392 15 434 1.19e-18 88.6
MS.gene87536.t1 MTR_7g027175 23.851 348 243 8 50 393 63 392 2.74e-18 87.0
MS.gene87536.t1 MTR_3g465980 26.978 278 181 8 55 324 68 331 2.77e-18 87.4
MS.gene87536.t1 MTR_7g084940 27.437 277 185 8 53 324 67 332 8.11e-18 85.9
MS.gene87536.t1 MTR_2g089755 24.760 416 251 17 24 396 37 433 1.40e-17 85.1
MS.gene87536.t1 MTR_4g097090 24.668 377 223 15 55 398 65 413 1.84e-17 84.7
MS.gene87536.t1 MTR_2g089670 26.368 402 250 13 22 392 43 429 3.35e-17 84.0
MS.gene87536.t1 MTR_7g014230 27.143 420 239 17 18 396 22 415 4.28e-17 83.6
MS.gene87536.t1 MTR_3g086020 27.000 400 234 18 55 441 61 415 7.60e-17 82.4
MS.gene87536.t1 MTR_3g465990 26.523 279 181 10 55 324 68 331 8.07e-17 82.8
MS.gene87536.t1 MTR_7g014330 25.118 422 245 16 18 396 20 413 1.07e-16 82.4
MS.gene87536.t1 MTR_5g076700 24.342 304 197 10 25 310 24 312 2.04e-16 81.6
MS.gene87536.t1 MTR_2g089650 24.299 428 258 18 8 396 21 421 3.24e-16 80.9
MS.gene87536.t1 MTR_7g015860 23.007 439 259 16 4 393 5 413 3.67e-16 80.9
MS.gene87536.t1 MTR_7g014250 26.596 376 224 16 52 396 59 413 3.85e-16 80.9
MS.gene87536.t1 MTR_4g055170 25.908 413 256 16 55 440 69 458 6.78e-16 80.1
MS.gene87536.t1 MTR_6g015850 24.508 457 281 18 10 430 21 449 1.11e-15 79.3
MS.gene87536.t1 MTR_3g071320 27.414 321 187 13 41 334 42 343 1.78e-15 78.6
MS.gene87536.t1 MTR_7g114520 22.901 393 254 16 25 393 25 392 2.01e-15 78.6
MS.gene87536.t1 MTR_1g040175 26.690 281 184 10 50 324 59 323 2.60e-15 78.2
MS.gene87536.t1 MTR_7g114490 23.423 444 286 15 25 439 24 442 2.89e-15 78.2
MS.gene87536.t1 MTR_6g015320 23.724 392 268 11 55 428 66 444 9.72e-15 76.3
MS.gene87536.t1 MTR_7g084910 26.786 280 171 10 53 317 68 328 9.47e-14 73.2
MS.gene87536.t1 MTR_7g014160 25.000 460 270 17 15 422 19 455 1.82e-13 72.4
MS.gene87536.t1 MTR_3g087640 23.992 471 302 17 1 428 5 462 2.51e-13 72.0
MS.gene87536.t1 MTR_6g053810 22.523 333 202 12 49 357 65 365 2.83e-13 72.0
MS.gene87536.t1 MTR_3g049140 26.667 180 114 6 235 399 27 203 6.92e-13 68.9
MS.gene87536.t1 MTR_1g053315 22.162 370 240 16 43 393 70 410 3.44e-12 68.6
MS.gene87536.t1 MTR_5g090810 22.892 415 254 15 55 424 66 459 1.02e-11 67.0
MS.gene87536.t1 MTR_7g014200 23.377 462 284 17 15 428 19 458 1.03e-11 67.0
MS.gene87536.t1 MTR_7g014610 23.608 449 293 13 15 422 18 457 1.15e-11 67.0
MS.gene87536.t1 MTR_7g085930 21.649 388 243 10 52 399 56 422 2.75e-11 65.9
MS.gene87536.t1 MTR_2g015430 22.506 391 254 13 19 395 31 386 3.57e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87536.t1 AT5G67150 39.474 456 249 12 1 440 1 445 4.28e-99 304
MS.gene87536.t1 AT3G50300 41.364 440 224 15 1 422 1 424 5.57e-95 293
MS.gene87536.t1 AT3G50280 37.416 449 259 10 1 437 1 439 4.20e-90 280
MS.gene87536.t1 AT5G67160 37.387 444 247 10 6 441 12 432 9.66e-88 274
MS.gene87536.t1 AT5G42830 36.591 440 255 10 15 441 21 449 1.03e-85 270
MS.gene87536.t1 AT3G50270 37.168 452 258 11 6 441 7 448 1.37e-85 269
MS.gene87536.t1 AT5G07850 39.551 445 240 13 15 442 22 454 6.62e-84 265
MS.gene87536.t1 AT5G07860 37.037 459 249 12 4 440 8 448 1.77e-82 261
MS.gene87536.t1 AT5G38130 37.844 436 243 12 1 422 21 442 2.76e-82 261
MS.gene87536.t1 AT5G07870 36.000 475 248 14 4 442 8 462 1.02e-74 241
MS.gene87536.t1 AT2G39980 33.472 481 246 13 16 442 21 481 3.87e-70 230
MS.gene87536.t1 AT5G01210 35.096 416 211 11 66 441 73 469 3.34e-68 224
MS.gene87536.t1 AT3G50280 37.304 319 184 6 125 437 1 309 1.20e-61 202
MS.gene87536.t1 AT3G50290 41.241 274 148 6 122 390 4 269 4.72e-54 182
MS.gene87536.t1 AT5G23940 30.110 455 288 13 4 437 3 448 6.00e-50 177
MS.gene87536.t1 AT5G48930 27.182 401 270 11 41 431 39 427 1.42e-32 128
MS.gene87536.t1 AT3G48720 27.747 364 229 13 42 393 44 385 2.40e-24 105
MS.gene87536.t1 AT5G57840 26.196 439 272 18 11 422 9 422 1.19e-22 100
MS.gene87536.t1 AT5G41040 25.248 404 258 15 52 439 82 457 2.98e-22 99.4
MS.gene87536.t1 AT5G41040 25.248 404 258 15 52 439 66 441 3.42e-22 99.0
MS.gene87536.t1 AT3G29670 23.423 444 298 13 3 422 8 433 8.96e-21 94.7
MS.gene87536.t1 AT5G63560 26.271 354 213 15 55 393 61 381 7.79e-20 91.7
MS.gene87536.t1 AT3G03480 26.895 409 242 18 25 408 33 409 3.27e-19 90.1
MS.gene87536.t1 AT3G29590 25.980 408 274 13 4 395 8 403 2.67e-18 87.4
MS.gene87536.t1 AT1G65450 26.667 285 184 12 53 325 70 341 1.74e-17 84.7
MS.gene87536.t1 AT1G24430 25.481 416 262 16 41 442 45 426 1.96e-17 84.7
MS.gene87536.t1 AT3G26040 25.472 424 244 17 45 441 51 429 2.92e-17 84.0
MS.gene87536.t1 AT3G29635 24.771 436 271 16 21 422 26 438 3.31e-17 84.0
MS.gene87536.t1 AT5G23970 25.670 448 286 19 4 437 3 417 3.88e-17 83.6
MS.gene87536.t1 AT2G40230 24.494 445 304 16 1 432 1 426 4.17e-17 83.6
MS.gene87536.t1 AT3G29680 23.590 390 262 10 52 422 59 431 1.36e-16 82.0
MS.gene87536.t1 AT1G28680 25.672 409 239 19 15 395 18 389 7.38e-16 79.7
MS.gene87536.t1 AT5G16410 24.372 398 247 17 41 414 77 444 8.97e-16 79.7
MS.gene87536.t1 AT5G39090 25.632 277 170 10 135 392 140 399 1.17e-15 79.3
MS.gene87536.t1 AT1G28680 25.904 332 199 13 15 325 18 323 2.92e-15 77.8
MS.gene87536.t1 AT5G07080 25.846 325 201 13 17 322 28 331 7.26e-15 76.6
MS.gene87536.t1 AT1G27620 24.422 389 258 15 55 431 58 422 1.20e-14 76.3
MS.gene87536.t1 AT2G25150 25.275 364 233 15 50 396 62 403 1.33e-14 75.9
MS.gene87536.t1 AT3G30280 25.061 411 250 19 54 441 61 436 1.72e-14 75.5
MS.gene87536.t1 AT5G61160 21.810 431 282 16 20 422 31 434 2.23e-14 75.5
MS.gene87536.t1 AT1G03390 25.068 367 226 18 48 393 69 407 1.37e-12 69.7
MS.gene87536.t1 AT3G47170 27.302 315 189 14 58 351 73 368 3.63e-12 68.6
MS.gene87536.t1 AT1G78990 25.141 354 230 16 53 393 65 396 3.87e-12 68.6
MS.gene87536.t1 AT5G39050 22.635 296 187 9 149 415 160 442 5.72e-12 67.8
MS.gene87536.t1 AT1G32910 23.133 415 262 18 53 432 63 455 8.77e-11 64.3

Find 90 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCATGGTTTGATGTTTA+TGG 0.113342 4.1:-75628275 MS.gene87536:CDS
CCATTACCAGAAAATTATTT+TGG 0.134239 4.1:-75628506 MS.gene87536:CDS
ACTTGCTTCAACCAATAAAA+AGG 0.152814 4.1:-75629308 MS.gene87536:CDS
ATAGTTTGATCACTTGATTT+TGG 0.265670 4.1:+75629361 None:intergenic
TTGTCTTCCACATGAGATTC+TGG 0.280245 4.1:-75628144 MS.gene87536:CDS
AAAACTTTGTTTATCAAATA+GGG 0.285623 4.1:-75628723 MS.gene87536:CDS
AACACTAGAGCGTTGGTTTC+CGG 0.290488 4.1:-75628837 MS.gene87536:CDS
GATGTTTATGGTAATGATTT+TGG 0.308658 4.1:-75628263 MS.gene87536:CDS
TTCTTGTGGATCAAATTGTT+TGG 0.335214 4.1:+75628577 None:intergenic
TAAGAAATCCAAGCTTTGTT+AGG 0.345177 4.1:-75628340 MS.gene87536:CDS
AGTGTCTCAGCCGTTGGATT+TGG 0.357834 4.1:+75629254 None:intergenic
AATGAAAGTTGGGGAGTTGT+TGG 0.379006 4.1:-75628456 MS.gene87536:CDS
AAATGCAATTATAAGTTGTA+TGG 0.380673 4.1:-75628483 MS.gene87536:CDS
ACCTCTCACGTTGCATTAGT+TGG 0.383610 4.1:+75628082 None:intergenic
TGGATTTGGTTAGCTTCAAC+TGG 0.386379 4.1:+75629268 None:intergenic
AAACCATGGAGAACTGCTAA+TGG 0.390568 4.1:+75628286 None:intergenic
GTATGGTTAAAATGAAAGTT+GGG 0.393088 4.1:-75628466 MS.gene87536:CDS
GAAACCAACGCTCTAGTGTT+GGG 0.395003 4.1:+75628840 None:intergenic
TGTATGGTTAAAATGAAAGT+TGG 0.395906 4.1:-75628467 MS.gene87536:CDS
TATGGCATTTCATCAATTCA+TGG 0.407954 4.1:-75628907 MS.gene87536:CDS
CTTTGTTAGGCTTGCTAACA+TGG 0.414224 4.1:-75628327 MS.gene87536:CDS
CTAGTTGACGGTATGTTCAT+TGG 0.417115 4.1:-75628968 MS.gene87536:CDS
GCCAGCAAGAGGTTGGAAAA+AGG 0.417931 4.1:+75629213 None:intergenic
AGTGTTTGCCAGAATAGAAA+AGG 0.423519 4.1:-75628186 MS.gene87536:CDS
GAGGTACATAGGTGGGTTCT+AGG 0.423951 4.1:+75629077 None:intergenic
TTTAACCATGTTGTCGTTGA+CGG 0.436978 4.1:-75628938 MS.gene87536:CDS
GACACAACATTCATGAATTG+AGG 0.451357 4.1:+75628104 None:intergenic
GTTGGGGAGTTGTTGGAGGA+AGG 0.453345 4.1:-75628449 MS.gene87536:CDS
TTTAACTCATCTTTGGTGCT+CGG 0.456890 4.1:-75628609 MS.gene87536:CDS
CGTAGTGGATATAAAGCAAA+TGG 0.458289 4.1:-75628218 MS.gene87536:CDS
CTATTAAATACTTCTTCTTG+TGG 0.458740 4.1:+75628563 None:intergenic
AATACGATTTCCTTTCACTT+TGG 0.459710 4.1:-75628798 MS.gene87536:CDS
GTATTTAATAGAGTCGTGAT+AGG 0.460162 4.1:-75628551 MS.gene87536:CDS
ACCTGCATTGTTGCATATAA+CGG 0.463614 4.1:+75629147 None:intergenic
TTAGCAAGCCTAACAAAGCT+TGG 0.467551 4.1:+75628332 None:intergenic
TGAAGAATCACTACGAGTCT+TGG 0.477110 4.1:-75628364 MS.gene87536:CDS
GGGGAGTTGTTGGAGGAAGG+TGG 0.485977 4.1:-75628446 MS.gene87536:CDS
TATCCACTACGAACTGCTAC+AGG 0.486958 4.1:+75628230 None:intergenic
AATAGAGTCGTGATAGGAGT+TGG 0.491643 4.1:-75628545 MS.gene87536:CDS
CATGTGGAAGACAAACTTGA+AGG 0.493205 4.1:+75628153 None:intergenic
AGGCACATCAAAACCATTGT+TGG 0.495948 4.1:-75629008 MS.gene87536:CDS
AAACTATTGATTTGACTCCA+TGG 0.507614 4.1:-75629345 MS.gene87536:CDS
GAAAGTTGGGGAGTTGTTGG+AGG 0.511101 4.1:-75628453 MS.gene87536:CDS
AACTATTGATTTGACTCCAT+GGG 0.516810 4.1:-75629344 MS.gene87536:CDS
CAACAAAAGAATCACTCTGA+TGG 0.518700 4.1:-75628773 MS.gene87536:CDS
CACATCAAAACCATTGTTGG+CGG 0.519877 4.1:-75629005 MS.gene87536:CDS
TGCTGGCCGTCTCAAAATAA+CGG 0.522868 4.1:-75629197 MS.gene87536:CDS
ATGAATTGATGAAATGCCAT+AGG 0.530467 4.1:+75628909 None:intergenic
ACATGAGATTCTGGAGGCAA+TGG 0.532230 4.1:-75628135 MS.gene87536:CDS
ATGGCATTTCATCAATTCAT+GGG 0.535218 4.1:-75628906 MS.gene87536:CDS
AAAGTGAAAGGAAATCGTAT+TGG 0.539286 4.1:+75628800 None:intergenic
ACAGAGAGTGTCTCAGCCGT+TGG 0.539317 4.1:+75629248 None:intergenic
GGAAACCAACGCTCTAGTGT+TGG 0.539690 4.1:+75628839 None:intergenic
TGAGACGGCCAGCAAGAGGT+TGG 0.540839 4.1:+75629206 None:intergenic
GAGGAAGGTGGACTTTGTAA+AGG 0.546587 4.1:-75628434 MS.gene87536:CDS
TCCGTTATATGCAACAATGC+AGG 0.551070 4.1:-75629148 MS.gene87536:CDS
ATTGGCCGTTGAACACCATC+CGG 0.551218 4.1:+75628818 None:intergenic
ATTGTTGGCGGTTCAAGTAA+CGG 0.553835 4.1:-75628993 MS.gene87536:CDS
AACAAAAGAATCACTCTGAT+GGG 0.558688 4.1:-75628772 MS.gene87536:CDS
GTGTTTGCCAGAATAGAAAA+GGG 0.560054 4.1:-75628185 MS.gene87536:CDS
GGTAATGGTGGAACTAACCT+TGG 0.563399 4.1:+75628521 None:intergenic
GGTTTCCGGATGGTGTTCAA+CGG 0.567162 4.1:-75628823 MS.gene87536:CDS
ACACAACATTCATGAATTGA+GGG 0.567594 4.1:+75628105 None:intergenic
AAACCAACGCTCTAGTGTTG+GGG 0.573903 4.1:+75628841 None:intergenic
ATCGACAATTGGAGGTACAT+AGG 0.574863 4.1:+75629066 None:intergenic
TCTCTCCACTGATACAATCA+AGG 0.580251 4.1:-75629386 MS.gene87536:CDS
TCGTTGACGGAAAGTCCCTA+TGG 0.585370 4.1:-75628925 MS.gene87536:CDS
TGGTGCCTTGATTGTATCAG+TGG 0.587630 4.1:+75629381 None:intergenic
GTTACTTGAACCGCCAACAA+TGG 0.593750 4.1:+75628995 None:intergenic
TTTGGTTAGCTTCAACTGGA+CGG 0.595328 4.1:+75629272 None:intergenic
TATCCATTAGCAGTTCTCCA+TGG 0.597915 4.1:-75628289 MS.gene87536:CDS
TATGGTTAAAATGAAAGTTG+GGG 0.603920 4.1:-75628465 MS.gene87536:CDS
TGAATTGATGAAATGCCATA+GGG 0.604499 4.1:+75628910 None:intergenic
AAGTATTCTCAGCTGCAGCA+TGG 0.612633 4.1:+75629113 None:intergenic
AGTAGAAATCGAAGATCCCA+TGG 0.614089 4.1:+75629328 None:intergenic
CAAGTAACGGAACTAGTTGA+CGG 0.616630 4.1:-75628980 MS.gene87536:CDS
GTGATTCTTCAACTTCGCAT+CGG 0.618663 4.1:+75628376 None:intergenic
ATCAGTGGAGAGAACTCGTA+CGG 0.619099 4.1:+75629396 None:intergenic
ACAATTGGAGGTACATAGGT+GGG 0.626645 4.1:+75629070 None:intergenic
AACAAAGTTAGAAGCTACGA+AGG 0.627886 4.1:-75629028 MS.gene87536:CDS
ACTTTCCGTCAACGACAACA+TGG 0.629179 4.1:+75628933 None:intergenic
CTAGAGCGTTGGTTTCCGGA+TGG 0.634274 4.1:-75628833 MS.gene87536:CDS
AACCAACGCTCTAGTGTTGG+GGG 0.634809 4.1:+75628842 None:intergenic
GACAATTGGAGGTACATAGG+TGG 0.636969 4.1:+75629069 None:intergenic
TCTTCCACATGAGATTCTGG+AGG 0.645425 4.1:-75628141 MS.gene87536:CDS
TTACCATAAACATCAAACCA+TGG 0.659822 4.1:+75628272 None:intergenic
AAGCCTGTAGCAGTTCGTAG+TGG 0.668630 4.1:-75628233 MS.gene87536:CDS
TCGTGATAGGAGTTGGACCA+AGG 0.676348 4.1:-75628538 MS.gene87536:CDS
TTGAAGCTAACCAAATCCAA+CGG 0.678953 4.1:-75629264 MS.gene87536:CDS
ATTGCCTCCAGAATCTCATG+TGG 0.679505 4.1:+75628137 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAACTTTGTTTATCAAAT+AGG - chr4.1:75628776-75628795 MS.gene87536:CDS 15.0%
!!! AAAACTTTGTTTATCAAATA+GGG - chr4.1:75628777-75628796 MS.gene87536:CDS 15.0%
!! AAATGCAATTATAAGTTGTA+TGG - chr4.1:75629017-75629036 MS.gene87536:CDS 20.0%
!! TTAAAAATCAATGAAGAGAT+CGG - chr4.1:75628838-75628857 MS.gene87536:CDS 20.0%
! ACAAAAAATGAATCGACAAT+TGG + chr4.1:75628448-75628467 None:intergenic 25.0%
! CTATTAAATACTTCTTCTTG+TGG + chr4.1:75628940-75628959 None:intergenic 25.0%
! GTATGGTTAAAATGAAAGTT+GGG - chr4.1:75629034-75629053 MS.gene87536:CDS 25.0%
! TATGGTTAAAATGAAAGTTG+GGG - chr4.1:75629035-75629054 MS.gene87536:CDS 25.0%
! TGTATGGTTAAAATGAAAGT+TGG - chr4.1:75629033-75629052 MS.gene87536:CDS 25.0%
!! ATAAAGAAGACCCTTTTTAT+TGG + chr4.1:75628206-75628225 None:intergenic 25.0%
!! ATAGTTTGATCACTTGATTT+TGG + chr4.1:75628142-75628161 None:intergenic 25.0%
!! CCATTACCAGAAAATTATTT+TGG - chr4.1:75628994-75629013 MS.gene87536:CDS 25.0%
!! GCATTTCCAAAATAATTTTC+TGG + chr4.1:75629003-75629022 None:intergenic 25.0%
!!! AAATAATTTTCTGGTAATGG+TGG + chr4.1:75628994-75629013 None:intergenic 25.0%
!!! CCAAAATAATTTTCTGGTAA+TGG + chr4.1:75628997-75629016 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG - chr4.1:75629237-75629256 MS.gene87536:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG - chr4.1:75629242-75629261 MS.gene87536:CDS 25.0%
!!! TTGTAGAGAAGATATTTTGA+TGG + chr4.1:75628866-75628885 None:intergenic 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG - chr4.1:75629241-75629260 MS.gene87536:CDS 25.0%
AAAAATGAATCGACAATTGG+AGG + chr4.1:75628445-75628464 None:intergenic 30.0%
AAAGTGAAAGGAAATCGTAT+TGG + chr4.1:75628703-75628722 None:intergenic 30.0%
AACAAAAGAATCACTCTGAT+GGG - chr4.1:75628728-75628747 MS.gene87536:CDS 30.0%
AATACGATTTCCTTTCACTT+TGG - chr4.1:75628702-75628721 MS.gene87536:CDS 30.0%
ACACAACATTCATGAATTGA+GGG + chr4.1:75629398-75629417 None:intergenic 30.0%
ACTTGCTTCAACCAATAAAA+AGG - chr4.1:75628192-75628211 MS.gene87536:CDS 30.0%
ATGAATTGATGAAATGCCAT+AGG + chr4.1:75628594-75628613 None:intergenic 30.0%
ATGGCATTTCATCAATTCAT+GGG - chr4.1:75628594-75628613 MS.gene87536:CDS 30.0%
CTTGCTTCAACCAATAAAAA+GGG - chr4.1:75628193-75628212 MS.gene87536:CDS 30.0%
GTATTTAATAGAGTCGTGAT+AGG - chr4.1:75628949-75628968 MS.gene87536:CDS 30.0%
TATGGCATTTCATCAATTCA+TGG - chr4.1:75628593-75628612 MS.gene87536:CDS 30.0%
TGAATTGATGAAATGCCATA+GGG + chr4.1:75628593-75628612 None:intergenic 30.0%
TTACCATAAACATCAAACCA+TGG + chr4.1:75629231-75629250 None:intergenic 30.0%
TTCTTGTGGATCAAATTGTT+TGG + chr4.1:75628926-75628945 None:intergenic 30.0%
! TATACTACCCTTTTCTATTC+TGG + chr4.1:75629325-75629344 None:intergenic 30.0%
!! AAACTATTGATTTGACTCCA+TGG - chr4.1:75628155-75628174 MS.gene87536:CDS 30.0%
!! AACTATTGATTTGACTCCAT+GGG - chr4.1:75628156-75628175 MS.gene87536:CDS 30.0%
!! TAAGAAATCCAAGCTTTGTT+AGG - chr4.1:75629160-75629179 MS.gene87536:CDS 30.0%
!! TATGGTAATGATTTTGGATG+GGG - chr4.1:75629243-75629262 MS.gene87536:CDS 30.0%
AACAAAGTTAGAAGCTACGA+AGG - chr4.1:75628472-75628491 MS.gene87536:CDS 35.0%
ACCTGCATTGTTGCATATAA+CGG + chr4.1:75628356-75628375 None:intergenic 35.0%
AGTGTTTGCCAGAATAGAAA+AGG - chr4.1:75629314-75629333 MS.gene87536:CDS 35.0%
CAACAAAAGAATCACTCTGA+TGG - chr4.1:75628727-75628746 MS.gene87536:CDS 35.0%
GACACAACATTCATGAATTG+AGG + chr4.1:75629399-75629418 None:intergenic 35.0%
GTGTTTGCCAGAATAGAAAA+GGG - chr4.1:75629315-75629334 MS.gene87536:CDS 35.0%
TCTCCATGGTTTGATGTTTA+TGG - chr4.1:75629225-75629244 MS.gene87536:CDS 35.0%
TTGAAGCTAACCAAATCCAA+CGG - chr4.1:75628236-75628255 MS.gene87536:CDS 35.0%
TTTAACCATGTTGTCGTTGA+CGG - chr4.1:75628562-75628581 MS.gene87536:CDS 35.0%
! TTTTGTTGCTCCAAAGTGAA+AGG + chr4.1:75628715-75628734 None:intergenic 35.0%
!! AAGCGCTTTTAACTCATCTT+TGG - chr4.1:75628884-75628903 MS.gene87536:CDS 35.0%
!! CGTAGTGGATATAAAGCAAA+TGG - chr4.1:75629282-75629301 MS.gene87536:CDS 35.0%
!! TTTAACTCATCTTTGGTGCT+CGG - chr4.1:75628891-75628910 MS.gene87536:CDS 35.0%
!!! AGTGTTCCGTTATTTTGAGA+CGG + chr4.1:75628312-75628331 None:intergenic 35.0%
AAACCATGGAGAACTGCTAA+TGG + chr4.1:75629217-75629236 None:intergenic 40.0%
AATAGAGTCGTGATAGGAGT+TGG - chr4.1:75628955-75628974 MS.gene87536:CDS 40.0%
AATGAAAGTTGGGGAGTTGT+TGG - chr4.1:75629044-75629063 MS.gene87536:CDS 40.0%
AGGCACATCAAAACCATTGT+TGG - chr4.1:75628492-75628511 MS.gene87536:CDS 40.0%
AGTAGAAATCGAAGATCCCA+TGG + chr4.1:75628175-75628194 None:intergenic 40.0%
ATTGTTGGCGGTTCAAGTAA+CGG - chr4.1:75628507-75628526 MS.gene87536:CDS 40.0%
CACATCAAAACCATTGTTGG+CGG - chr4.1:75628495-75628514 MS.gene87536:CDS 40.0%
CATGTGGAAGACAAACTTGA+AGG + chr4.1:75629350-75629369 None:intergenic 40.0%
CTAGTTGACGGTATGTTCAT+TGG - chr4.1:75628532-75628551 MS.gene87536:CDS 40.0%
GTGATTCTTCAACTTCGCAT+CGG + chr4.1:75629127-75629146 None:intergenic 40.0%
TATCCATTAGCAGTTCTCCA+TGG - chr4.1:75629211-75629230 MS.gene87536:CDS 40.0%
TCCGTTATATGCAACAATGC+AGG - chr4.1:75628352-75628371 MS.gene87536:CDS 40.0%
TCTCTCCACTGATACAATCA+AGG - chr4.1:75628114-75628133 MS.gene87536:CDS 40.0%
TGAAGAATCACTACGAGTCT+TGG - chr4.1:75629136-75629155 MS.gene87536:CDS 40.0%
TTAGCAAGCCTAACAAAGCT+TGG + chr4.1:75629171-75629190 None:intergenic 40.0%
TTGGAAAAAGGTAAGAGCAG+AGG + chr4.1:75628278-75628297 None:intergenic 40.0%
TTGTCTTCCACATGAGATTC+TGG - chr4.1:75629356-75629375 MS.gene87536:CDS 40.0%
! ACAATTGGAGGTACATAGGT+GGG + chr4.1:75628433-75628452 None:intergenic 40.0%
! ATCGACAATTGGAGGTACAT+AGG + chr4.1:75628437-75628456 None:intergenic 40.0%
! CAAGTAACGGAACTAGTTGA+CGG - chr4.1:75628520-75628539 MS.gene87536:CDS 40.0%
!! CTTTGTTAGGCTTGCTAACA+TGG - chr4.1:75629173-75629192 MS.gene87536:CDS 40.0%
!! TGGATTTGGTTAGCTTCAAC+TGG + chr4.1:75628235-75628254 None:intergenic 40.0%
!! TTTGGTTAGCTTCAACTGGA+CGG + chr4.1:75628231-75628250 None:intergenic 40.0%
AAACCAACGCTCTAGTGTTG+GGG + chr4.1:75628662-75628681 None:intergenic 45.0%
AAGTATTCTCAGCTGCAGCA+TGG + chr4.1:75628390-75628409 None:intergenic 45.0%
ACTTTCCGTCAACGACAACA+TGG + chr4.1:75628570-75628589 None:intergenic 45.0%
ATCAGTGGAGAGAACTCGTA+CGG + chr4.1:75628107-75628126 None:intergenic 45.0%
ATTGCCTCCAGAATCTCATG+TGG + chr4.1:75629366-75629385 None:intergenic 45.0%
GAAACCAACGCTCTAGTGTT+GGG + chr4.1:75628663-75628682 None:intergenic 45.0%
GTTACTTGAACCGCCAACAA+TGG + chr4.1:75628508-75628527 None:intergenic 45.0%
TATCCACTACGAACTGCTAC+AGG + chr4.1:75629273-75629292 None:intergenic 45.0%
TCTTCCACATGAGATTCTGG+AGG - chr4.1:75629359-75629378 MS.gene87536:CDS 45.0%
TGCTGGCCGTCTCAAAATAA+CGG - chr4.1:75628303-75628322 MS.gene87536:CDS 45.0%
TGGTGCCTTGATTGTATCAG+TGG + chr4.1:75628122-75628141 None:intergenic 45.0%
! ACATGAGATTCTGGAGGCAA+TGG - chr4.1:75629365-75629384 MS.gene87536:CDS 45.0%
! ACCTTTTTCCAACCTCTTGC+TGG - chr4.1:75628286-75628305 MS.gene87536:CDS 45.0%
! GACAATTGGAGGTACATAGG+TGG + chr4.1:75628434-75628453 None:intergenic 45.0%
! GAGGAAGGTGGACTTTGTAA+AGG - chr4.1:75629066-75629085 MS.gene87536:CDS 45.0%
! GGTAATGGTGGAACTAACCT+TGG + chr4.1:75628982-75629001 None:intergenic 45.0%
!! AACACTAGAGCGTTGGTTTC+CGG - chr4.1:75628663-75628682 MS.gene87536:CDS 45.0%
AAGCCTGTAGCAGTTCGTAG+TGG - chr4.1:75629267-75629286 MS.gene87536:CDS 50.0%
AGTGTCTCAGCCGTTGGATT+TGG + chr4.1:75628249-75628268 None:intergenic 50.0%
ATTGGCCGTTGAACACCATC+CGG + chr4.1:75628685-75628704 None:intergenic 50.0%
GAAAGTTGGGGAGTTGTTGG+AGG - chr4.1:75629047-75629066 MS.gene87536:CDS 50.0%
GCCAGCAAGAGGTTGGAAAA+AGG + chr4.1:75628290-75628309 None:intergenic 50.0%
GGAAACCAACGCTCTAGTGT+TGG + chr4.1:75628664-75628683 None:intergenic 50.0%
GGTTTCCGGATGGTGTTCAA+CGG - chr4.1:75628677-75628696 MS.gene87536:CDS 50.0%
TCGTGATAGGAGTTGGACCA+AGG - chr4.1:75628962-75628981 MS.gene87536:CDS 50.0%
TCGTTGACGGAAAGTCCCTA+TGG - chr4.1:75628575-75628594 MS.gene87536:CDS 50.0%
! AACCAACGCTCTAGTGTTGG+GGG + chr4.1:75628661-75628680 None:intergenic 50.0%
! GAGGTACATAGGTGGGTTCT+AGG + chr4.1:75628426-75628445 None:intergenic 50.0%
!!! ATTTTGAGACGGCCAGCAAG+AGG + chr4.1:75628301-75628320 None:intergenic 50.0%
AACCCCCAACACTAGAGCGT+TGG - chr4.1:75628656-75628675 MS.gene87536:CDS 55.0%
GTTGGGGAGTTGTTGGAGGA+AGG - chr4.1:75629051-75629070 MS.gene87536:CDS 55.0%
!! ACAGAGAGTGTCTCAGCCGT+TGG + chr4.1:75628255-75628274 None:intergenic 55.0%
!! CTAGAGCGTTGGTTTCCGGA+TGG - chr4.1:75628667-75628686 MS.gene87536:CDS 55.0%
GGGGAGTTGTTGGAGGAAGG+TGG - chr4.1:75629054-75629073 MS.gene87536:CDS 60.0%
TGAGACGGCCAGCAAGAGGT+TGG + chr4.1:75628297-75628316 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 75628097 75629425 75628097 ID=MS.gene87536
chr4.1 mRNA 75628097 75629425 75628097 ID=MS.gene87536.t1;Parent=MS.gene87536
chr4.1 exon 75628097 75629425 75628097 ID=MS.gene87536.t1.exon1;Parent=MS.gene87536.t1
chr4.1 CDS 75628097 75629425 75628097 ID=cds.MS.gene87536.t1;Parent=MS.gene87536.t1
Gene Sequence

>MS.gene87536

ATGGGAACCGTACGAGTTCTCTCCACTGATACAATCAAGGCACCAAAATCAAGTGATCAAACTATTGATTTGACTCCATGGGATCTTCGATTTCTACTTGCTTCAACCAATAAAAAGGGTCTTCTTTATCACCGTCCAGTTGAAGCTAACCAAATCCAACGGCTGAGACACTCTCTGTCCTCTGCTCTTACCTTTTTCCAACCTCTTGCTGGCCGTCTCAAAATAACGGAACACTCAGACAACACTGTCTCGTGTTCCGTTATATGCAACAATGCAGGTGTACTCTTTATCCATGCTGCAGCTGAGAATACTTGTGTTGCTGACATCCTAGAACCCACCTATGTACCTCCAATTGTCGATTCATTTTTTGTTTTAAACAAAGTTAGAAGCTACGAAGGCACATCAAAACCATTGTTGGCGGTTCAAGTAACGGAACTAGTTGACGGTATGTTCATTGGTTGCTCATTTAACCATGTTGTCGTTGACGGAAAGTCCCTATGGCATTTCATCAATTCATGGGCTGAAATTTCACGTAGTTGTTGTCATCATCAAATATCTAAACCCCCAACACTAGAGCGTTGGTTTCCGGATGGTGTTCAACGGCCAATACGATTTCCTTTCACTTTGGAGCAACAAAAGAATCACTCTGATGGGTTGAGTTTTTCGTCGTTTGATGATGAAAAACTTTGTTTATCAAATAGGGTATTTCATTTCACAAAAGAGAAAATTGTGCAGCTGAAATTAAAAATCAATGAAGAGATCGGTACCATCAAAATATCTTCTCTACAAGCGCTTTTAACTCATCTTTGGTGCTCGGTGATGCGTTCCAAACAATTTGATCCACAAGAAGAAGTATTTAATAGAGTCGTGATAGGAGTTGGACCAAGGTTAGTTCCACCATTACCAGAAAATTATTTTGGAAATGCAATTATAAGTTGTATGGTTAAAATGAAAGTTGGGGAGTTGTTGGAGGAAGGTGGACTTTGTAAAGGTGCTTGCGAGATGAACAAGTTAATTGCTTCACACACCGATGCGAAGTTGAAGAATCACTACGAGTCTTGGTTAAGAAATCCAAGCTTTGTTAGGCTTGCTAACATGGTAAAAAATAATTTCATATCCATTAGCAGTTCTCCATGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAGCCTGTAGCAGTTCGTAGTGGATATAAAGCAAATGGACTTATTACAGTGTTTGCCAGAATAGAAAAGGGTAGTATAGACCTTCAAGTTTGTCTTCCACATGAGATTCTGGAGGCAATGGAAAATGACCCTCAATTCATGAATGTTGTGTCCAACTAA
Protein sequence

>MS.gene87536.t1

MGTVRVLSTDTIKAPKSSDQTIDLTPWDLRFLLASTNKKGLLYHRPVEANQIQRLRHSLSSALTFFQPLAGRLKITEHSDNTVSCSVICNNAGVLFIHAAAENTCVADILEPTYVPPIVDSFFVLNKVRSYEGTSKPLLAVQVTELVDGMFIGCSFNHVVVDGKSLWHFINSWAEISRSCCHHQISKPPTLERWFPDGVQRPIRFPFTLEQQKNHSDGLSFSSFDDEKLCLSNRVFHFTKEKIVQLKLKINEEIGTIKISSLQALLTHLWCSVMRSKQFDPQEEVFNRVVIGVGPRLVPPLPENYFGNAIISCMVKMKVGELLEEGGLCKGACEMNKLIASHTDAKLKNHYESWLRNPSFVRLANMVKNNFISISSSPWFDVYGNDFGWGKPVAVRSGYKANGLITVFARIEKGSIDLQVCLPHEILEAMENDPQFMNVVSN