AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070019

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070019.t1 MTR_4g022670 92.906 437 31 0 28 464 6 442 0.0 842
MS.gene070019.t1 MTR_4g022800 81.963 438 78 1 28 464 6 443 0.0 753
MS.gene070019.t1 MTR_4g022720 79.634 437 83 1 28 464 6 436 0.0 728
MS.gene070019.t1 MTR_7g026860 77.117 437 97 1 28 464 6 439 0.0 711
MS.gene070019.t1 MTR_4g022760 74.600 437 67 1 28 464 6 398 0.0 667
MS.gene070019.t1 MTR_4g022825 72.874 435 117 1 28 462 6 439 0.0 658
MS.gene070019.t1 MTR_3g088420 66.133 437 140 2 28 464 6 434 0.0 595
MS.gene070019.t1 MTR_3g026310 64.865 444 149 2 28 464 6 449 0.0 585
MS.gene070019.t1 MTR_8g041770 58.705 448 163 6 28 464 6 442 0.0 515
MS.gene070019.t1 MTR_0190s0020 58.166 447 166 7 28 463 6 442 3.30e-180 511
MS.gene070019.t1 MTR_4g094842 56.889 450 165 9 30 461 8 446 5.00e-165 473
MS.gene070019.t1 MTR_4g094835 52.902 448 180 8 30 459 8 442 4.20e-157 453
MS.gene070019.t1 MTR_4g022290 51.762 454 179 12 30 461 8 443 8.44e-145 421
MS.gene070019.t1 MTR_6g016815 51.716 437 137 7 28 464 20 382 1.40e-141 411
MS.gene070019.t1 MTR_4g094838 49.448 453 177 9 30 459 5 428 6.41e-141 411
MS.gene070019.t1 MTR_4g094828 51.322 454 196 12 27 463 5 450 2.34e-140 410
MS.gene070019.t1 MTR_8g079710 51.467 443 183 10 30 454 8 436 3.60e-140 409
MS.gene070019.t1 MTR_4g094832 55.789 380 141 7 94 459 60 426 8.64e-139 405
MS.gene070019.t1 MTR_3g101900 38.902 437 242 11 40 463 24 448 7.07e-91 283
MS.gene070019.t1 MTR_3g101930 37.838 444 246 9 38 462 22 454 4.17e-87 274
MS.gene070019.t1 MTR_8g040760 64.904 208 66 3 28 229 6 212 4.58e-86 263
MS.gene070019.t1 MTR_7g026850 72.254 173 47 1 45 217 7 178 6.38e-85 259
MS.gene070019.t1 MTR_3g102020 38.269 439 244 9 38 460 21 448 1.89e-84 267
MS.gene070019.t1 MTR_7g026770 66.162 198 39 1 163 360 132 301 3.31e-83 259
MS.gene070019.t1 MTR_3g101970 37.273 440 237 10 38 462 21 436 5.19e-82 260
MS.gene070019.t1 MTR_3g101960 37.986 437 243 10 38 458 21 445 6.01e-82 261
MS.gene070019.t1 MTR_3g102010 36.652 442 252 10 38 463 22 451 2.57e-80 256
MS.gene070019.t1 MTR_8g040780 56.944 216 89 2 247 458 12 227 1.16e-77 243
MS.gene070019.t1 MTR_5g081960 30.081 492 281 12 25 463 5 486 2.01e-55 192
MS.gene070019.t1 MTR_4g015180 31.697 489 253 13 38 464 19 488 2.14e-55 192
MS.gene070019.t1 MTR_3g070170 30.687 466 255 13 38 459 25 466 9.63e-55 190
MS.gene070019.t1 MTR_4g094825 49.765 213 89 6 24 221 60 269 1.13e-53 185
MS.gene070019.t1 MTR_4g094825 51.656 151 60 2 311 459 248 387 2.28e-44 160
MS.gene070019.t1 MTR_6g045553 28.352 455 269 11 45 459 34 471 3.48e-48 172
MS.gene070019.t1 MTR_6g034485 60.606 132 44 1 211 342 2 125 2.19e-47 159
MS.gene070019.t1 MTR_3g102030 34.242 330 175 7 95 419 28 320 6.19e-46 162
MS.gene070019.t1 MTR_7g026800 53.801 171 48 4 230 399 16 156 8.26e-46 158
MS.gene070019.t1 MTR_4g022780 53.216 171 49 4 230 399 17 157 3.11e-45 155
MS.gene070019.t1 MTR_6g034495 58.394 137 47 3 76 202 28 164 1.20e-43 151
MS.gene070019.t1 MTR_7g026780 62.162 111 18 1 354 464 26 112 1.99e-39 138
MS.gene070019.t1 MTR_7g026830 75.294 85 21 0 104 188 14 98 4.99e-37 131
MS.gene070019.t1 MTR_7g026870 86.765 68 4 1 311 373 84 151 3.46e-32 120
MS.gene070019.t1 MTR_8g075610 25.330 379 264 10 77 450 47 411 4.47e-29 118
MS.gene070019.t1 MTR_4g007540 26.020 392 249 13 77 450 56 424 3.36e-28 116
MS.gene070019.t1 MTR_8g096530 28.235 425 233 17 71 458 53 442 4.85e-28 116
MS.gene070019.t1 MTR_2g105330 26.053 380 263 11 77 450 56 423 8.47e-28 115
MS.gene070019.t1 MTR_2g064495 25.789 380 264 10 77 450 56 423 1.13e-27 115
MS.gene070019.t1 MTR_3g098980 28.249 354 209 12 77 415 66 389 2.84e-27 114
MS.gene070019.t1 MTR_7g026880 90.385 52 5 0 412 463 2 53 1.30e-26 102
MS.gene070019.t1 MTR_3g026950 26.098 387 216 16 70 415 47 404 2.23e-23 102
MS.gene070019.t1 MTR_4g013050 27.147 361 201 13 72 415 48 363 6.12e-23 100
MS.gene070019.t1 MTR_1g081660 23.490 447 289 15 46 464 31 452 6.95e-20 92.4
MS.gene070019.t1 MTR_7g027175 25.000 396 253 12 71 450 62 429 7.02e-19 89.0
MS.gene070019.t1 MTR_3g049150 25.127 394 264 11 74 448 52 433 8.63e-19 89.0
MS.gene070019.t1 MTR_6g015830 24.561 456 298 14 26 454 18 454 1.19e-17 85.5
MS.gene070019.t1 MTR_2g089755 23.937 447 288 17 46 454 37 469 3.00e-17 84.3
MS.gene070019.t1 MTR_2g089765 24.757 412 251 15 46 414 39 434 3.14e-17 84.3
MS.gene070019.t1 MTR_2g089670 25.000 408 248 15 44 414 43 429 4.51e-17 84.0
MS.gene070019.t1 MTR_7g014330 24.096 415 258 13 40 418 20 413 5.71e-17 83.6
MS.gene070019.t1 MTR_2g089650 23.632 457 298 16 19 440 3 443 1.36e-16 82.4
MS.gene070019.t1 MTR_3g087640 23.656 465 309 14 27 454 11 466 1.86e-16 82.0
MS.gene070019.t1 MTR_4g097090 23.404 423 279 12 30 420 4 413 5.87e-16 80.5
MS.gene070019.t1 MTR_7g114470 22.685 432 284 16 47 450 25 434 6.72e-16 80.1
MS.gene070019.t1 MTR_5g076700 22.477 436 289 15 47 455 24 437 7.37e-16 80.1
MS.gene070019.t1 MTR_3g465980 25.497 302 196 9 77 366 68 352 1.06e-15 79.7
MS.gene070019.t1 MTR_7g015860 23.855 415 248 16 43 415 25 413 2.11e-15 78.6
MS.gene070019.t1 MTR_6g015320 25.293 427 269 14 43 436 26 435 3.90e-15 77.8
MS.gene070019.t1 MTR_4g127650 23.291 395 256 15 77 444 66 440 7.65e-15 77.0
MS.gene070019.t1 MTR_7g014250 23.684 418 254 14 41 418 21 413 9.18e-15 76.6
MS.gene070019.t1 MTR_7g084910 26.730 318 182 14 75 363 68 363 9.97e-15 76.3
MS.gene070019.t1 MTR_7g114520 21.189 387 268 12 47 415 25 392 1.42e-14 75.9
MS.gene070019.t1 MTR_7g085930 23.117 385 259 9 65 421 47 422 1.45e-14 75.9
MS.gene070019.t1 MTR_3g465990 25.418 299 200 10 77 366 68 352 5.84e-14 74.3
MS.gene070019.t1 MTR_1g040195 24.292 424 260 19 47 450 23 405 5.91e-14 73.9
MS.gene070019.t1 MTR_7g084940 25.362 276 188 9 75 344 67 330 7.18e-14 73.9
MS.gene070019.t1 MTR_7g056087 21.939 392 270 13 76 450 57 429 9.41e-14 73.6
MS.gene070019.t1 MTR_7g085990 24.041 391 248 13 65 421 43 418 1.00e-13 73.6
MS.gene070019.t1 MTR_1g040315 25.249 301 190 13 47 331 23 304 1.02e-13 73.2
MS.gene070019.t1 MTR_7g086000 25.067 375 235 16 80 428 63 417 1.68e-13 72.8
MS.gene070019.t1 MTR_3g465930 25.850 294 191 11 77 361 69 344 2.12e-13 72.4
MS.gene070019.t1 MTR_1g040175 26.449 276 181 11 77 346 64 323 3.12e-13 72.0
MS.gene070019.t1 MTR_3g465910 26.224 286 184 11 77 352 68 336 3.58e-13 72.0
MS.gene070019.t1 MTR_5g090830 23.636 385 239 13 65 415 49 412 1.26e-12 70.1
MS.gene070019.t1 MTR_5g090810 23.117 385 241 13 65 415 49 412 1.44e-12 69.7
MS.gene070019.t1 MTR_6g053810 24.911 281 185 10 77 346 71 336 2.12e-12 69.3
MS.gene070019.t1 MTR_7g014200 23.953 430 255 16 37 418 19 424 3.30e-12 68.6
MS.gene070019.t1 MTR_7g014610 23.294 425 266 15 77 464 68 469 1.23e-11 67.0
MS.gene070019.t1 MTR_5g090880 21.818 440 282 15 65 463 49 467 8.59e-11 64.3
MS.gene070019.t1 MTR_3g112180 22.299 435 304 15 26 450 16 426 9.09e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070019.t1 AT5G67150 40.984 427 230 11 44 459 27 442 6.79e-95 294
MS.gene070019.t1 AT3G50300 41.981 424 215 14 36 444 17 424 1.84e-91 285
MS.gene070019.t1 AT5G67160 39.310 435 241 11 33 463 17 432 6.02e-89 278
MS.gene070019.t1 AT3G50280 39.070 430 235 11 44 460 25 440 1.64e-88 277
MS.gene070019.t1 AT5G42830 37.273 440 252 11 37 463 21 449 3.49e-85 269
MS.gene070019.t1 AT3G50270 38.149 443 250 11 34 463 17 448 1.53e-84 267
MS.gene070019.t1 AT5G07850 38.876 445 243 12 37 464 22 454 3.03e-81 259
MS.gene070019.t1 AT5G07860 37.136 447 240 10 37 462 22 448 7.07e-80 255
MS.gene070019.t1 AT5G07870 37.611 452 247 12 37 464 22 462 7.73e-76 245
MS.gene070019.t1 AT5G38130 36.219 439 250 12 23 444 17 442 4.88e-75 243
MS.gene070019.t1 AT2G39980 32.079 505 258 14 20 464 2 481 2.79e-66 221
MS.gene070019.t1 AT3G50280 37.812 320 179 7 149 460 3 310 2.69e-62 205
MS.gene070019.t1 AT5G01210 32.941 425 208 12 88 463 73 469 1.29e-60 205
MS.gene070019.t1 AT5G23940 31.435 439 265 14 44 459 23 448 3.07e-53 186
MS.gene070019.t1 AT3G50290 40.511 274 150 6 144 412 4 269 6.60e-52 177
MS.gene070019.t1 AT5G48930 26.913 379 260 9 75 447 54 421 1.81e-30 122
MS.gene070019.t1 AT3G48720 28.490 351 213 15 77 415 61 385 1.71e-22 99.8
MS.gene070019.t1 AT5G57840 25.513 439 281 18 29 444 7 422 6.95e-22 98.2
MS.gene070019.t1 AT5G41040 25.762 361 224 13 71 415 63 395 7.95e-22 98.2
MS.gene070019.t1 AT5G41040 25.762 361 224 13 71 415 79 411 1.00e-21 97.8
MS.gene070019.t1 AT2G19070 24.781 456 278 20 49 462 19 451 5.81e-21 95.5
MS.gene070019.t1 AT5G63560 25.989 354 226 13 71 415 55 381 3.17e-19 90.1
MS.gene070019.t1 AT3G29670 22.603 438 295 14 42 454 25 443 4.07e-18 87.0
MS.gene070019.t1 AT5G39080 25.499 451 275 19 36 444 14 445 1.24e-15 79.3
MS.gene070019.t1 AT3G29635 24.422 389 263 12 46 414 29 406 1.86e-15 79.0
MS.gene070019.t1 AT2G25150 24.658 365 230 13 74 418 64 403 8.19e-15 77.0
MS.gene070019.t1 AT2G40230 26.016 369 242 13 63 419 42 391 2.86e-14 75.1
MS.gene070019.t1 AT1G28680 24.507 355 221 17 80 417 65 389 3.25e-14 75.1
MS.gene070019.t1 AT1G24430 23.256 387 250 13 63 436 45 397 3.54e-14 74.7
MS.gene070019.t1 AT3G29680 24.304 395 253 15 74 444 59 431 2.83e-13 72.0
MS.gene070019.t1 AT5G07080 27.046 281 171 12 78 344 71 331 3.78e-13 71.6
MS.gene070019.t1 AT5G47980 24.818 411 244 19 76 459 60 432 4.83e-13 71.2
MS.gene070019.t1 AT5G61160 24.364 275 182 10 157 415 139 403 7.29e-13 70.9
MS.gene070019.t1 AT1G28680 25.166 302 196 14 80 371 65 346 9.23e-13 70.1
MS.gene070019.t1 AT5G23970 25.400 437 265 22 30 445 7 403 1.91e-12 69.3
MS.gene070019.t1 AT1G78990 25.496 353 230 15 75 415 65 396 3.05e-12 68.9
MS.gene070019.t1 AT5G16410 23.896 385 239 16 63 421 77 433 6.34e-12 67.8
MS.gene070019.t1 AT1G24420 25.507 345 205 15 87 417 75 381 3.07e-11 65.5
MS.gene070019.t1 AT1G24420 25.507 345 205 15 87 417 75 381 3.07e-11 65.5
MS.gene070019.t1 AT5G39050 22.424 330 213 12 138 437 126 442 8.02e-11 64.3

Find 112 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCATGGTTTGATGTTTA+TGG 0.113342 4.3:+75920511 MS.gene070019:CDS
GCTTGCTTCAACCAACAAAA+AGG 0.220488 4.3:+75919478 MS.gene070019:CDS
CCGTTACCAGAAGATTATTT+TGG 0.221902 4.3:+75920280 MS.gene070019:CDS
AATGGATAAGAAATTATTAT+TGG 0.233186 4.3:-75920482 None:intergenic
CGTAGTGGATATAAAATAAA+TGG 0.258298 4.3:+75920568 MS.gene070019:CDS
AGTGTCTCAGCCGTTGGATT+TGG 0.276668 4.3:-75919532 None:intergenic
TAAGAAATCCAAGCTTTGTT+AGG 0.280992 4.3:+75920446 MS.gene070019:CDS
ATGTCTTCCTTACGAGGTTT+TGG 0.299283 4.3:+75920642 MS.gene070019:CDS
CATACGATTTCCTTTCACTA+TGG 0.304291 4.3:+75919988 MS.gene070019:CDS
GATGTTTATGGTAATGATTT+CGG 0.343194 4.3:+75920523 MS.gene070019:CDS
GGAGATCCCATGGAGTTAAA+TGG 0.348693 4.3:-75919448 None:intergenic
GTGTCTCAGCCGTTGGATTT+GGG 0.349706 4.3:-75919531 None:intergenic
TTCAACCATGTTGTCGTTGA+AGG 0.349859 4.3:+75919848 MS.gene070019:CDS
GAAACCAACGCTCTAGTGTT+GGG 0.360883 4.3:-75919946 None:intergenic
TATCCACTACGAACTGCTAT+AGG 0.366106 4.3:-75920556 None:intergenic
ATTGTATGATCACTTGATTT+TGG 0.375965 4.3:-75919425 None:intergenic
GAGTTTCCAAAATAATCTTC+TGG 0.384510 4.3:-75920286 None:intergenic
GGGAAGTGACCCACATTTCA+TGG 0.400457 4.3:+75920672 MS.gene070019:CDS
ATGGGAAGTTGAATACCATT+TGG 0.405055 4.3:-75919968 None:intergenic
AAACCATGGAGAACTACTAA+TGG 0.406093 4.3:-75920500 None:intergenic
GGAATTGTAAATGTATTTGC+TGG 0.411545 4.3:+75920589 MS.gene070019:CDS
GATTATATACTTCTTCTTGT+GGG 0.433778 4.3:-75920222 None:intergenic
TGTATAGTCAAAATGAAAGT+TGG 0.440205 4.3:+75920319 MS.gene070019:CDS
TATGGTAATGATTTCGGATG+GGG 0.444025 4.3:+75920529 MS.gene070019:CDS
AAGCGCTATTAACTCACCTT+TGG 0.445268 4.3:+75920170 MS.gene070019:CDS
GACGAAGGTGGATTATATAA+AGG 0.446121 4.3:+75920352 MS.gene070019:CDS
AATGAAAGTTGGTGAGTTGT+TGG 0.448624 4.3:+75920330 MS.gene070019:CDS
TGTGGCATTTCATTAATTCA+TGG 0.451210 4.3:+75919879 MS.gene070019:CDS
TGGATTTGGGTTTCTACCAC+CGG 0.460917 4.3:-75919518 None:intergenic
AAGAAATCCAAGCTTTGTTA+GGG 0.462160 4.3:+75920447 MS.gene070019:CDS
GTGGCATTTCATTAATTCAT+GGG 0.463542 4.3:+75919880 MS.gene070019:CDS
ATTTAACTCCATGGGATCTC+CGG 0.463819 4.3:+75919450 MS.gene070019:CDS
TACTTGTGTTGATGACATCT+TGG 0.466067 4.3:+75919691 MS.gene070019:CDS
CATGAAAGTGCTGAATTAGT+TGG 0.469381 4.3:-75920704 None:intergenic
GCCTGCATTGTTGCATATAA+CGG 0.471630 4.3:-75919639 None:intergenic
AGATTATATACTTCTTCTTG+TGG 0.484423 4.3:-75920223 None:intergenic
TGGTTGAAGCAAGCAGAAAC+CGG 0.484511 4.3:-75919469 None:intergenic
CCAAAATAATCTTCTGGTAA+CGG 0.486289 4.3:-75920280 None:intergenic
AATCTAGTCGTGATAGGAGT+TGG 0.488221 4.3:+75920241 MS.gene070019:CDS
TGGGTCAAATTGTTTGCAAC+GGG 0.488474 4.3:-75920203 None:intergenic
CTAGCAACCCTAACAAAGCT+TGG 0.494801 4.3:-75920454 None:intergenic
GTATATAATCTAGTCGTGAT+AGG 0.497624 4.3:+75920235 MS.gene070019:CDS
TTCGCCATGGACCACCGCGC+CGG 0.498907 4.3:-75919320 None:intergenic
GAGAGTACCGCAACGGTCAC+TGG 0.501901 4.3:-75919344 None:intergenic
TTCAACGACAACATGGTTGA+AGG 0.503002 4.3:-75919846 None:intergenic
GGAAACCAACGCTCTAGTGT+TGG 0.511281 4.3:-75919947 None:intergenic
CATGCCGGCGCGGTGGTCCA+TGG 0.512724 4.3:+75919316 None:intergenic
TGAAGAATCACTATGAGTCT+TGG 0.513376 4.3:+75920422 MS.gene070019:CDS
CATAGTGAAAGGAAATCGTA+TGG 0.513721 4.3:-75919987 None:intergenic
GGGTCTTCTTTATCACCATC+CGG 0.514663 4.3:+75919499 MS.gene070019:CDS
CTAGAGCGTTGGTTTCCAAA+TGG 0.515960 4.3:+75919953 MS.gene070019:CDS
AGGCACGTCAAAACCATTGT+TGG 0.517049 4.3:+75919778 MS.gene070019:CDS
TTTATGGTAATGATTTCGGA+TGG 0.520125 4.3:+75920527 MS.gene070019:CDS
ATACAATCCATTTAACTCCA+TGG 0.520586 4.3:+75919441 MS.gene070019:CDS
CGTAAGGAAGACATACTTGA+AGG 0.521339 4.3:-75920633 None:intergenic
AGTGGAGAGCGATGGTGAGA+GGG 0.532685 4.3:-75919374 None:intergenic
TGTCGGCCGTCTTGAAATAA+CGG 0.534950 4.3:+75919589 MS.gene070019:CDS
GTGGGTCAAATTGTTTGCAA+CGG 0.538778 4.3:-75920204 None:intergenic
TGGTGCCTTGATTGTATCAG+TGG 0.540456 4.3:-75919405 None:intergenic
TACAATCCATTTAACTCCAT+GGG 0.542338 4.3:+75919442 MS.gene070019:CDS
TCCGTTATATGCAACAATGC+AGG 0.542601 4.3:+75919638 MS.gene070019:CDS
TATCCATTAGTAGTTCTCCA+TGG 0.544589 4.3:+75920497 MS.gene070019:CDS
GGTGAGTTGTTGGACGAAGG+TGG 0.550907 4.3:+75920340 MS.gene070019:CDS
AGCAGAAACCGGAGATCCCA+TGG 0.551613 4.3:-75919458 None:intergenic
ATTTCAAGACGGCCGACAAG+AGG 0.553153 4.3:-75919584 None:intergenic
ATAGAGAGTGTCTCAGCCGT+TGG 0.554838 4.3:-75919538 None:intergenic
ATTGCCTCCAAAACCTCGTA+AGG 0.558716 4.3:-75920649 None:intergenic
TTATGGTAATGATTTCGGAT+GGG 0.560500 4.3:+75920528 MS.gene070019:CDS
AACAAAATAGCCATTGTGAT+GGG 0.565478 4.3:+75920014 MS.gene070019:CDS
CAGTGGAGAGCGATGGTGAG+AGG 0.565525 4.3:-75919375 None:intergenic
GTCACGACATCCATGAAATG+TGG 0.565781 4.3:-75920682 None:intergenic
GAAATCGAAAATCAATGAAG+AGG 0.568871 4.3:+75920120 MS.gene070019:CDS
AAACCAACGCTCTAGTGTTG+GGG 0.569521 4.3:-75919945 None:intergenic
ACAATCCACTGATACAATCA+AGG 0.569770 4.3:+75919400 MS.gene070019:CDS
GTATTTGCTGGAATAGAAGA+AGG 0.570455 4.3:+75920601 MS.gene070019:CDS
ACTTGTGTTGATGACATCTT+GGG 0.572753 4.3:+75919692 MS.gene070019:CDS
GGCGAAACATGCCGGCGCGG+TGG 0.572870 4.3:+75919309 None:intergenic
CAACAAAATAGCCATTGTGA+TGG 0.573773 4.3:+75920013 MS.gene070019:CDS
AACGGTCACTGGCTTCGCCA+TGG 0.578214 4.3:-75919333 None:intergenic
TCAAGTATGTCTTCCTTACG+AGG 0.584114 4.3:+75920636 MS.gene070019:CDS
CAAGTTAATTGCTTCACACA+CGG 0.586291 4.3:+75920390 MS.gene070019:CDS
CAAGTAACGGAGCTAGTTGA+CGG 0.586570 4.3:+75919806 MS.gene070019:CDS
ATTGTTGGCTGTTCAAGTAA+CGG 0.588367 4.3:+75919793 MS.gene070019:CDS
GTTGGTGAGTTGTTGGACGA+AGG 0.590545 4.3:+75920337 MS.gene070019:CDS
TTTGGGTTTCTACCACCGGA+TGG 0.595045 4.3:-75919514 None:intergenic
GAAATATCACGTGGTTGTTG+TGG 0.597765 4.3:+75919905 MS.gene070019:CDS
AGTGTTCCGTTATTTCAAGA+CGG 0.599596 4.3:-75919595 None:intergenic
GGTAACGGTGGAACAAACCT+TGG 0.603404 4.3:-75920265 None:intergenic
AACGGGTGACAAAGCACCAA+AGG 0.603630 4.3:-75920186 None:intergenic
ACTTTCCTTCAACGACAACA+TGG 0.607457 4.3:-75919853 None:intergenic
GTATCAGTGGATTGTATCAG+TGG 0.611091 4.3:-75919392 None:intergenic
TAGGTTATTTCATTTCACAA+AGG 0.626286 4.3:+75920081 MS.gene070019:CDS
AAGTATTCTCCGCTACAGCA+TGG 0.627484 4.3:-75919673 None:intergenic
AACCAACGCTCTAGTGTTGG+GGG 0.632370 4.3:-75919944 None:intergenic
GTTACTTGAACAGCCAACAA+TGG 0.633211 4.3:-75919791 None:intergenic
ATAGTGAAAGGAAATCGTAT+GGG 0.637742 4.3:-75919986 None:intergenic
AAATAATCTTCTGGTAACGG+TGG 0.643941 4.3:-75920277 None:intergenic
TCACGACATCCATGAAATGT+GGG 0.644749 4.3:-75920681 None:intergenic
TCGTGATAGGAGTTGGACCA+AGG 0.645786 4.3:+75920248 MS.gene070019:CDS
TCCGGCGAAACATGCCGGCG+CGG 0.647663 4.3:+75919306 None:intergenic
TGGTAGAAACCCAAATCCAA+CGG 0.650160 4.3:+75919522 MS.gene070019:CDS
TTACCATAAACATCAAACCA+TGG 0.659822 4.3:-75920514 None:intergenic
AAACCTATAGCAGTTCGTAG+TGG 0.661852 4.3:+75920553 MS.gene070019:CDS
GTGGAGAGCGATGGTGAGAG+GGG 0.662034 4.3:-75919373 None:intergenic
ACTCTTTATCCATGCTGTAG+CGG 0.670104 4.3:+75919664 MS.gene070019:CDS
TCTTCTTTATCACCATCCGG+TGG 0.677054 4.3:+75919502 MS.gene070019:CDS
GGCGAAGCCAGTGACCGTTG+CGG 0.682452 4.3:+75919337 MS.gene070019:CDS
TGAATTAATGAAATGCCACA+CGG 0.688433 4.3:-75919876 None:intergenic
GTTGTGCGAGAGTACCGCAA+CGG 0.717449 4.3:-75919351 None:intergenic
ATTGTATCAGTGGAGAGCGA+TGG 0.718102 4.3:-75919382 None:intergenic
TCATGGGCTGAAATATCACG+TGG 0.731649 4.3:+75919896 MS.gene070019:CDS
TCGTTGAAGGAAAGTCCGTG+TGG 0.753987 4.3:+75919861 MS.gene070019:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGGATAAGAAATTATTAT+TGG - chr4.3:75920485-75920504 None:intergenic 15.0%
!!! AAAAACTTTGTTTATCAAAT+AGG + chr4.3:75920062-75920081 MS.gene070019:CDS 15.0%
! AAAAATGAATCGACAATTAG+AGG - chr4.3:75919731-75919750 None:intergenic 25.0%
! AGATTATATACTTCTTCTTG+TGG - chr4.3:75920226-75920245 None:intergenic 25.0%
! GATTATATACTTCTTCTTGT+GGG - chr4.3:75920225-75920244 None:intergenic 25.0%
! TGTATAGTCAAAATGAAAGT+TGG + chr4.3:75920319-75920338 MS.gene070019:CDS 25.0%
!! ATAGTTTTTATCTCCGATAA+AGG - chr4.3:75919756-75919775 None:intergenic 25.0%
!! ATTGTATGATCACTTGATTT+TGG - chr4.3:75919428-75919447 None:intergenic 25.0%
!! GATGTTTATGGTAATGATTT+CGG + chr4.3:75920523-75920542 MS.gene070019:CDS 25.0%
!! TAGGTTATTTCATTTCACAA+AGG + chr4.3:75920081-75920100 MS.gene070019:CDS 25.0%
!!! CGTAGTGGATATAAAATAAA+TGG + chr4.3:75920568-75920587 MS.gene070019:CDS 25.0%
!!! GATTCATTTTTTGCCTTTAT+CGG + chr4.3:75919740-75919759 MS.gene070019:CDS 25.0%
AACAAAATAGCCATTGTGAT+GGG + chr4.3:75920014-75920033 MS.gene070019:CDS 30.0%
AAGAGGTTGAAAAAAGGAAA+GGG - chr4.3:75919570-75919589 None:intergenic 30.0%
ATACAATCCATTTAACTCCA+TGG + chr4.3:75919441-75919460 MS.gene070019:CDS 30.0%
ATAGTGAAAGGAAATCGTAT+GGG - chr4.3:75919989-75920008 None:intergenic 30.0%
ATCGGAGATAAAAACTATGA+AGG + chr4.3:75919758-75919777 MS.gene070019:CDS 30.0%
CCAAAATAATCTTCTGGTAA+CGG - chr4.3:75920283-75920302 None:intergenic 30.0%
GAAATCGAAAATCAATGAAG+AGG + chr4.3:75920120-75920139 MS.gene070019:CDS 30.0%
GAGTTTCCAAAATAATCTTC+TGG - chr4.3:75920289-75920308 None:intergenic 30.0%
GGAATTGTAAATGTATTTGC+TGG + chr4.3:75920589-75920608 MS.gene070019:CDS 30.0%
GTATATAATCTAGTCGTGAT+AGG + chr4.3:75920235-75920254 MS.gene070019:CDS 30.0%
GTGGCATTTCATTAATTCAT+GGG + chr4.3:75919880-75919899 MS.gene070019:CDS 30.0%
TACAATCCATTTAACTCCAT+GGG + chr4.3:75919442-75919461 MS.gene070019:CDS 30.0%
TGAATTAATGAAATGCCACA+CGG - chr4.3:75919879-75919898 None:intergenic 30.0%
TGTGGCATTTCATTAATTCA+TGG + chr4.3:75919879-75919898 MS.gene070019:CDS 30.0%
TTACCATAAACATCAAACCA+TGG - chr4.3:75920517-75920536 None:intergenic 30.0%
! ATAAAGAAGACCCTTTTTGT+TGG - chr4.3:75919492-75919511 None:intergenic 30.0%
! TTATGGTAATGATTTCGGAT+GGG + chr4.3:75920528-75920547 MS.gene070019:CDS 30.0%
! TTTATGGTAATGATTTCGGA+TGG + chr4.3:75920527-75920546 MS.gene070019:CDS 30.0%
!! AAGAAATCCAAGCTTTGTTA+GGG + chr4.3:75920447-75920466 MS.gene070019:CDS 30.0%
!! TAAGAAATCCAAGCTTTGTT+AGG + chr4.3:75920446-75920465 MS.gene070019:CDS 30.0%
!!! TTTTGTTGTTCCATAGTGAA+AGG - chr4.3:75920001-75920020 None:intergenic 30.0%
AAAAACTCAACCCATCACAA+TGG - chr4.3:75920027-75920046 None:intergenic 35.0%
AAACCATGGAGAACTACTAA+TGG - chr4.3:75920503-75920522 None:intergenic 35.0%
ACAATCCACTGATACAATCA+AGG + chr4.3:75919400-75919419 MS.gene070019:CDS 35.0%
ATGGGAAGTTGAATACCATT+TGG - chr4.3:75919971-75919990 None:intergenic 35.0%
CAACAAAATAGCCATTGTGA+TGG + chr4.3:75920013-75920032 MS.gene070019:CDS 35.0%
CAAGAGGTTGAAAAAAGGAA+AGG - chr4.3:75919571-75919590 None:intergenic 35.0%
CAAGTTAATTGCTTCACACA+CGG + chr4.3:75920390-75920409 MS.gene070019:CDS 35.0%
CATACGATTTCCTTTCACTA+TGG + chr4.3:75919988-75920007 MS.gene070019:CDS 35.0%
CATAGTGAAAGGAAATCGTA+TGG - chr4.3:75919990-75920009 None:intergenic 35.0%
CTTGCTTCAACCAACAAAAA+GGG + chr4.3:75919479-75919498 MS.gene070019:CDS 35.0%
TATCCATTAGTAGTTCTCCA+TGG + chr4.3:75920497-75920516 MS.gene070019:CDS 35.0%
TCTCCATGGTTTGATGTTTA+TGG + chr4.3:75920511-75920530 MS.gene070019:CDS 35.0%
TGAAGAATCACTATGAGTCT+TGG + chr4.3:75920422-75920441 MS.gene070019:CDS 35.0%
! AGTGTTCCGTTATTTCAAGA+CGG - chr4.3:75919598-75919617 None:intergenic 35.0%
! ATTGTTGGCTGTTCAAGTAA+CGG + chr4.3:75919793-75919812 MS.gene070019:CDS 35.0%
! CCGTTACCAGAAGATTATTT+TGG + chr4.3:75920280-75920299 MS.gene070019:CDS 35.0%
! CTAGTTGACGGTATTTTCAT+TGG + chr4.3:75919818-75919837 MS.gene070019:CDS 35.0%
! GTATTTGCTGGAATAGAAGA+AGG + chr4.3:75920601-75920620 MS.gene070019:CDS 35.0%
! TATGGTAATGATTTCGGATG+GGG + chr4.3:75920529-75920548 MS.gene070019:CDS 35.0%
!! AAATAATCTTCTGGTAACGG+TGG - chr4.3:75920280-75920299 None:intergenic 35.0%
!! AATGAAAGTTGGTGAGTTGT+TGG + chr4.3:75920330-75920349 MS.gene070019:CDS 35.0%
!! ACTTGTGTTGATGACATCTT+GGG + chr4.3:75919692-75919711 MS.gene070019:CDS 35.0%
!! GACGAAGGTGGATTATATAA+AGG + chr4.3:75920352-75920371 MS.gene070019:CDS 35.0%
!! TACTTGTGTTGATGACATCT+TGG + chr4.3:75919691-75919710 MS.gene070019:CDS 35.0%
!!! TCCTTTTTTCAACCTCTTGT+CGG + chr4.3:75919572-75919591 MS.gene070019:CDS 35.0%
AAACCTATAGCAGTTCGTAG+TGG + chr4.3:75920553-75920572 MS.gene070019:CDS 40.0%
AAGCGCTATTAACTCACCTT+TGG + chr4.3:75920170-75920189 MS.gene070019:CDS 40.0%
AATCTAGTCGTGATAGGAGT+TGG + chr4.3:75920241-75920260 MS.gene070019:CDS 40.0%
ACTCTTTATCCATGCTGTAG+CGG + chr4.3:75919664-75919683 MS.gene070019:CDS 40.0%
ACTTTCCTTCAACGACAACA+TGG - chr4.3:75919856-75919875 None:intergenic 40.0%
ATTTAACTCCATGGGATCTC+CGG + chr4.3:75919450-75919469 MS.gene070019:CDS 40.0%
CGTAAGGAAGACATACTTGA+AGG - chr4.3:75920636-75920655 None:intergenic 40.0%
GAAATATCACGTGGTTGTTG+TGG + chr4.3:75919905-75919924 MS.gene070019:CDS 40.0%
GCCTGCATTGTTGCATATAA+CGG - chr4.3:75919642-75919661 None:intergenic 40.0%
GCTTGCTTCAACCAACAAAA+AGG + chr4.3:75919478-75919497 MS.gene070019:CDS 40.0%
GTGGGTCAAATTGTTTGCAA+CGG - chr4.3:75920207-75920226 None:intergenic 40.0%
GTTACTTGAACAGCCAACAA+TGG - chr4.3:75919794-75919813 None:intergenic 40.0%
TATCCACTACGAACTGCTAT+AGG - chr4.3:75920559-75920578 None:intergenic 40.0%
TCAAGTATGTCTTCCTTACG+AGG + chr4.3:75920636-75920655 MS.gene070019:CDS 40.0%
TCACGACATCCATGAAATGT+GGG - chr4.3:75920684-75920703 None:intergenic 40.0%
TCCGTTATATGCAACAATGC+AGG + chr4.3:75919638-75919657 MS.gene070019:CDS 40.0%
TGGGTCAAATTGTTTGCAAC+GGG - chr4.3:75920206-75920225 None:intergenic 40.0%
TGGTAGAAACCCAAATCCAA+CGG + chr4.3:75919522-75919541 MS.gene070019:CDS 40.0%
TTCAACCATGTTGTCGTTGA+AGG + chr4.3:75919848-75919867 MS.gene070019:CDS 40.0%
TTCAACGACAACATGGTTGA+AGG - chr4.3:75919849-75919868 None:intergenic 40.0%
TTGAAAAAAGGAAAGGGCAG+AGG - chr4.3:75919564-75919583 None:intergenic 40.0%
! ATGTCTTCCTTACGAGGTTT+TGG + chr4.3:75920642-75920661 MS.gene070019:CDS 40.0%
!! GAGTTTTTCAGCGTCTAATG+AGG + chr4.3:75920039-75920058 MS.gene070019:CDS 40.0%
!! GTATCAGTGGATTGTATCAG+TGG - chr4.3:75919395-75919414 None:intergenic 40.0%
AAACCAACGCTCTAGTGTTG+GGG - chr4.3:75919948-75919967 None:intergenic 45.0%
AAGTATTCTCCGCTACAGCA+TGG - chr4.3:75919676-75919695 None:intergenic 45.0%
AGGCACGTCAAAACCATTGT+TGG + chr4.3:75919778-75919797 MS.gene070019:CDS 45.0%
ATTGCCTCCAAAACCTCGTA+AGG - chr4.3:75920652-75920671 None:intergenic 45.0%
ATTGTATCAGTGGAGAGCGA+TGG - chr4.3:75919385-75919404 None:intergenic 45.0%
CTAGCAACCCTAACAAAGCT+TGG - chr4.3:75920457-75920476 None:intergenic 45.0%
GAAACCAACGCTCTAGTGTT+GGG - chr4.3:75919949-75919968 None:intergenic 45.0%
GCCGACAAGAGGTTGAAAAA+AGG - chr4.3:75919576-75919595 None:intergenic 45.0%
GGAGATCCCATGGAGTTAAA+TGG - chr4.3:75919451-75919470 None:intergenic 45.0%
GGGTCTTCTTTATCACCATC+CGG + chr4.3:75919499-75919518 MS.gene070019:CDS 45.0%
GTCACGACATCCATGAAATG+TGG - chr4.3:75920685-75920704 None:intergenic 45.0%
TCTTCTTTATCACCATCCGG+TGG + chr4.3:75919502-75919521 MS.gene070019:CDS 45.0%
TGGTGCCTTGATTGTATCAG+TGG - chr4.3:75919408-75919427 None:intergenic 45.0%
TGGTTGAAGCAAGCAGAAAC+CGG - chr4.3:75919472-75919491 None:intergenic 45.0%
TGTCGGCCGTCTTGAAATAA+CGG + chr4.3:75919589-75919608 MS.gene070019:CDS 45.0%
! CAAGTAACGGAGCTAGTTGA+CGG + chr4.3:75919806-75919825 MS.gene070019:CDS 45.0%
! TCATGGGCTGAAATATCACG+TGG + chr4.3:75919896-75919915 MS.gene070019:CDS 45.0%
! TGGATTTGGGTTTCTACCAC+CGG - chr4.3:75919521-75919540 None:intergenic 45.0%
!! CTAGAGCGTTGGTTTCCAAA+TGG + chr4.3:75919953-75919972 MS.gene070019:CDS 45.0%
!! TCTTCCTTACGAGGTTTTGG+AGG + chr4.3:75920645-75920664 MS.gene070019:CDS 45.0%
!!! TACGAGGTTTTGGAGGCAAT+GGG + chr4.3:75920652-75920671 MS.gene070019:CDS 45.0%
!!! TTACGAGGTTTTGGAGGCAA+TGG + chr4.3:75920651-75920670 MS.gene070019:CDS 45.0%
AGTGTCTCAGCCGTTGGATT+TGG - chr4.3:75919535-75919554 None:intergenic 50.0%
ATTTCAAGACGGCCGACAAG+AGG - chr4.3:75919587-75919606 None:intergenic 50.0%
GGAAACCAACGCTCTAGTGT+TGG - chr4.3:75919950-75919969 None:intergenic 50.0%
GGGAAGTGACCCACATTTCA+TGG + chr4.3:75920672-75920691 MS.gene070019:CDS 50.0%
GTGTCTCAGCCGTTGGATTT+GGG - chr4.3:75919534-75919553 None:intergenic 50.0%
TCGTGATAGGAGTTGGACCA+AGG + chr4.3:75920248-75920267 MS.gene070019:CDS 50.0%
TCGTTGAAGGAAAGTCCGTG+TGG + chr4.3:75919861-75919880 MS.gene070019:CDS 50.0%
! AACCAACGCTCTAGTGTTGG+GGG - chr4.3:75919947-75919966 None:intergenic 50.0%
! AACGGGTGACAAAGCACCAA+AGG - chr4.3:75920189-75920208 None:intergenic 50.0%
! GGTAACGGTGGAACAAACCT+TGG - chr4.3:75920268-75920287 None:intergenic 50.0%
! GTTGGTGAGTTGTTGGACGA+AGG + chr4.3:75920337-75920356 MS.gene070019:CDS 50.0%
! TTTGGGTTTCTACCACCGGA+TGG - chr4.3:75919517-75919536 None:intergenic 50.0%
!! ATAGAGAGTGTCTCAGCCGT+TGG - chr4.3:75919541-75919560 None:intergenic 50.0%
AACCCCCAACACTAGAGCGT+TGG + chr4.3:75919942-75919961 MS.gene070019:CDS 55.0%
AGCAGAAACCGGAGATCCCA+TGG - chr4.3:75919461-75919480 None:intergenic 55.0%
AGTGGAGAGCGATGGTGAGA+GGG - chr4.3:75919377-75919396 None:intergenic 55.0%
GGTGAGTTGTTGGACGAAGG+TGG + chr4.3:75920340-75920359 MS.gene070019:CDS 55.0%
! ACGCTCTAGTGTTGGGGGTT+TGG - chr4.3:75919942-75919961 None:intergenic 55.0%
!! GTTGTGCGAGAGTACCGCAA+CGG - chr4.3:75919354-75919373 None:intergenic 55.0%
CAGTGGAGAGCGATGGTGAG+AGG - chr4.3:75919378-75919397 None:intergenic 60.0%
GTGGAGAGCGATGGTGAGAG+GGG - chr4.3:75919376-75919395 None:intergenic 60.0%
!! AACGGTCACTGGCTTCGCCA+TGG - chr4.3:75919336-75919355 None:intergenic 60.0%
!! GAGAGTACCGCAACGGTCAC+TGG - chr4.3:75919347-75919366 None:intergenic 60.0%
GGCGAAGCCAGTGACCGTTG+CGG + chr4.3:75919337-75919356 MS.gene070019:CDS 65.0%
TTCGCCATGGACCACCGCGC+CGG - chr4.3:75919323-75919342 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 75919317 75920711 75919317 ID=MS.gene070019
chr4.3 mRNA 75919317 75920711 75919317 ID=MS.gene070019.t1;Parent=MS.gene070019
chr4.3 exon 75919317 75920711 75919317 ID=MS.gene070019.t1.exon1;Parent=MS.gene070019.t1
chr4.3 CDS 75919317 75920711 75919317 ID=cds.MS.gene070019.t1;Parent=MS.gene070019.t1
Gene Sequence

>MS.gene070019

ATGCCGGCGCGGTGGTCCATGGCGAAGCCAGTGACCGTTGCGGTACTCTCGCACAACCCCTCTCACCATCGCTCTCCACTGATACAATCCACTGATACAATCAAGGCACCAAAATCAAGTGATCATACAATCCATTTAACTCCATGGGATCTCCGGTTTCTGCTTGCTTCAACCAACAAAAAGGGTCTTCTTTATCACCATCCGGTGGTAGAAACCCAAATCCAACGGCTGAGACACTCTCTATCCTCTGCCCTTTCCTTTTTTCAACCTCTTGTCGGCCGTCTTGAAATAACGGAACACTCAGACAACACTATCTCGTGTTCCGTTATATGCAACAATGCAGGCGTACTCTTTATCCATGCTGTAGCGGAGAATACTTGTGTTGATGACATCTTGGGATCAGTCTACGTTCCTCTAATTGTCGATTCATTTTTTGCCTTTATCGGAGATAAAAACTATGAAGGCACGTCAAAACCATTGTTGGCTGTTCAAGTAACGGAGCTAGTTGACGGTATTTTCATTGGTTGCTCCTTCAACCATGTTGTCGTTGAAGGAAAGTCCGTGTGGCATTTCATTAATTCATGGGCTGAAATATCACGTGGTTGTTGTGGTGATCAAATATCCAAACCCCCAACACTAGAGCGTTGGTTTCCAAATGGTATTCAACTTCCCATACGATTTCCTTTCACTATGGAACAACAAAATAGCCATTGTGATGGGTTGAGTTTTTCAGCGTCTAATGAGGAAAAACTTTGTTTATCAAATAGGTTATTTCATTTCACAAAGGAGAAAATCAGTCAACTGAAATCGAAAATCAATGAAGAGGTTAGTGCAATCAAAATATCATCTCTACAAGCGCTATTAACTCACCTTTGGTGCTTTGTCACCCGTTGCAAACAATTTGACCCACAAGAAGAAGTATATAATCTAGTCGTGATAGGAGTTGGACCAAGGTTTGTTCCACCGTTACCAGAAGATTATTTTGGAAACTCGATTATAAGTTGTATAGTCAAAATGAAAGTTGGTGAGTTGTTGGACGAAGGTGGATTATATAAAGGTGCTTGTGAGATGAACAAGTTAATTGCTTCACACACGGATGAGAAGTTGAAGAATCACTATGAGTCTTGGCTAAGAAATCCAAGCTTTGTTAGGGTTGCTAGCATGTCCAATAATAATTTCTTATCCATTAGTAGTTCTCCATGGTTTGATGTTTATGGTAATGATTTCGGATGGGGAAAACCTATAGCAGTTCGTAGTGGATATAAAATAAATGGAATTGTAAATGTATTTGCTGGAATAGAAGAAGGTAGTATTGACCTTCAAGTATGTCTTCCTTACGAGGTTTTGGAGGCAATGGGAAGTGACCCACATTTCATGGATGTCGTGACCAACTAA
Protein sequence

>MS.gene070019.t1

MPARWSMAKPVTVAVLSHNPSHHRSPLIQSTDTIKAPKSSDHTIHLTPWDLRFLLASTNKKGLLYHHPVVETQIQRLRHSLSSALSFFQPLVGRLEITEHSDNTISCSVICNNAGVLFIHAVAENTCVDDILGSVYVPLIVDSFFAFIGDKNYEGTSKPLLAVQVTELVDGIFIGCSFNHVVVEGKSVWHFINSWAEISRGCCGDQISKPPTLERWFPNGIQLPIRFPFTMEQQNSHCDGLSFSASNEEKLCLSNRLFHFTKEKISQLKSKINEEVSAIKISSLQALLTHLWCFVTRCKQFDPQEEVYNLVVIGVGPRFVPPLPEDYFGNSIISCIVKMKVGELLDEGGLYKGACEMNKLIASHTDEKLKNHYESWLRNPSFVRVASMSNNNFLSISSSPWFDVYGNDFGWGKPIAVRSGYKINGIVNVFAGIEEGSIDLQVCLPYEVLEAMGSDPHFMDVVTN