AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000763

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000763.t1 MTR_4g022800 88.262 443 51 1 1 442 1 443 0.0 810
MS.gene000763.t1 MTR_4g022720 88.462 442 45 1 1 442 1 436 0.0 808
MS.gene000763.t1 MTR_7g026860 85.520 442 61 1 1 442 1 439 0.0 785
MS.gene000763.t1 MTR_4g022670 82.805 442 76 0 1 442 1 442 0.0 764
MS.gene000763.t1 MTR_4g022825 74.603 441 111 1 1 441 1 440 0.0 681
MS.gene000763.t1 MTR_4g022760 75.792 442 63 1 1 442 1 398 0.0 674
MS.gene000763.t1 MTR_3g088420 68.100 442 133 2 1 442 1 434 0.0 613
MS.gene000763.t1 MTR_3g026310 66.815 449 142 2 1 442 1 449 0.0 602
MS.gene000763.t1 MTR_0190s0020 57.743 452 170 7 1 441 1 442 0.0 524
MS.gene000763.t1 MTR_8g041770 58.057 453 168 6 1 442 1 442 0.0 515
MS.gene000763.t1 MTR_4g094842 56.236 457 171 11 1 439 1 446 9.90e-168 479
MS.gene000763.t1 MTR_4g094835 51.868 455 188 8 1 437 1 442 1.33e-158 456
MS.gene000763.t1 MTR_6g016815 52.262 442 137 7 1 442 15 382 1.43e-144 417
MS.gene000763.t1 MTR_4g094828 50.873 458 200 12 1 441 1 450 2.58e-144 419
MS.gene000763.t1 MTR_4g022290 50.325 461 189 13 1 439 1 443 7.03e-143 416
MS.gene000763.t1 MTR_4g094838 48.796 457 182 9 4 437 1 428 2.04e-141 411
MS.gene000763.t1 MTR_8g079710 50.552 453 186 11 1 432 1 436 3.57e-141 411
MS.gene000763.t1 MTR_4g094832 50.588 425 177 7 33 437 15 426 6.23e-139 405
MS.gene000763.t1 MTR_3g101930 40.449 445 233 11 16 440 22 454 2.48e-94 292
MS.gene000763.t1 MTR_3g101900 40.138 436 238 10 18 441 24 448 3.53e-94 291
MS.gene000763.t1 MTR_7g026850 84.713 157 24 0 29 185 12 168 1.45e-93 280
MS.gene000763.t1 MTR_3g102020 39.367 442 241 10 16 441 21 451 5.76e-91 283
MS.gene000763.t1 MTR_7g026770 69.192 198 33 1 141 338 132 301 8.08e-89 272
MS.gene000763.t1 MTR_8g040760 64.789 213 68 3 1 207 1 212 2.04e-88 268
MS.gene000763.t1 MTR_3g101960 38.991 436 240 9 16 436 21 445 2.91e-88 276
MS.gene000763.t1 MTR_3g101970 38.041 439 235 9 16 440 21 436 3.38e-88 275
MS.gene000763.t1 MTR_3g102010 38.776 441 244 11 16 441 22 451 1.10e-87 275
MS.gene000763.t1 MTR_8g040780 57.604 217 88 2 224 436 11 227 6.13e-79 245
MS.gene000763.t1 MTR_4g015180 33.742 489 243 14 16 442 19 488 7.23e-61 206
MS.gene000763.t1 MTR_5g081960 31.435 474 271 10 16 441 19 486 6.21e-59 201
MS.gene000763.t1 MTR_3g070170 31.330 466 260 11 16 441 25 470 3.23e-58 198
MS.gene000763.t1 MTR_4g094825 50.794 189 84 5 17 199 84 269 6.29e-51 177
MS.gene000763.t1 MTR_4g094825 49.367 158 67 2 286 441 245 391 2.02e-44 159
MS.gene000763.t1 MTR_6g034495 61.940 134 43 2 54 179 28 161 1.47e-48 164
MS.gene000763.t1 MTR_3g102030 33.939 330 176 8 73 397 28 320 1.67e-47 166
MS.gene000763.t1 MTR_6g045553 28.753 473 283 13 4 437 14 471 2.74e-46 166
MS.gene000763.t1 MTR_7g026830 87.059 85 11 0 82 166 14 98 5.27e-46 154
MS.gene000763.t1 MTR_6g034485 59.091 132 46 1 189 320 2 125 1.23e-43 149
MS.gene000763.t1 MTR_7g026800 49.701 167 54 2 212 377 19 156 6.01e-42 147
MS.gene000763.t1 MTR_4g022780 48.066 181 61 4 200 377 7 157 1.90e-41 145
MS.gene000763.t1 MTR_7g026780 65.766 111 14 2 332 442 26 112 9.76e-39 136
MS.gene000763.t1 MTR_4g007540 26.292 445 272 17 16 432 12 428 1.37e-30 122
MS.gene000763.t1 MTR_8g075610 27.157 394 244 14 55 431 47 414 2.02e-28 116
MS.gene000763.t1 MTR_2g105330 26.546 388 257 11 55 431 56 426 6.06e-28 115
MS.gene000763.t1 MTR_7g026870 90.741 54 5 0 298 351 98 151 8.63e-28 108
MS.gene000763.t1 MTR_2g064495 26.546 388 257 11 55 431 56 426 8.93e-28 114
MS.gene000763.t1 MTR_8g096530 28.116 345 224 9 53 392 57 382 2.48e-25 108
MS.gene000763.t1 MTR_4g013050 28.846 364 185 13 53 393 51 363 1.14e-24 105
MS.gene000763.t1 MTR_3g026950 27.561 410 239 17 52 426 51 437 8.67e-24 103
MS.gene000763.t1 MTR_3g098980 25.990 404 232 16 55 431 66 429 1.93e-22 99.4
MS.gene000763.t1 MTR_3g049150 26.943 386 239 14 41 399 37 406 1.51e-20 94.0
MS.gene000763.t1 MTR_7g026880 71.154 52 15 0 390 441 2 53 1.96e-20 84.7
MS.gene000763.t1 MTR_3g086020 27.352 457 267 21 4 441 5 415 8.85e-19 88.6
MS.gene000763.t1 MTR_6g015830 27.412 456 272 18 10 430 21 452 9.44e-19 88.6
MS.gene000763.t1 MTR_7g027175 22.879 389 276 10 50 432 63 433 2.52e-18 87.0
MS.gene000763.t1 MTR_2g089765 23.235 439 269 15 3 392 15 434 3.09e-18 87.4
MS.gene000763.t1 MTR_2g089755 24.760 416 251 17 24 396 37 433 9.77e-18 85.5
MS.gene000763.t1 MTR_1g081660 24.103 390 252 15 24 393 31 396 2.14e-17 84.3
MS.gene000763.t1 MTR_2g089650 23.778 450 277 18 8 418 21 443 3.48e-17 84.0
MS.gene000763.t1 MTR_3g465980 27.698 278 179 8 55 324 68 331 4.64e-17 83.6
MS.gene000763.t1 MTR_3g465990 27.240 279 179 10 55 324 68 331 1.71e-16 81.6
MS.gene000763.t1 MTR_7g084940 25.683 366 229 15 45 392 52 392 6.70e-16 79.7
MS.gene000763.t1 MTR_4g055170 29.412 306 188 12 55 351 69 355 1.09e-15 79.3
MS.gene000763.t1 MTR_3g087640 24.628 471 299 17 1 428 5 462 1.28e-15 79.3
MS.gene000763.t1 MTR_3g028210 24.130 460 291 16 4 423 8 449 2.29e-15 78.2
MS.gene000763.t1 MTR_7g114560 32.386 176 96 8 25 185 24 191 7.41e-15 76.6
MS.gene000763.t1 MTR_7g015860 23.007 439 259 18 4 393 5 413 8.12e-15 76.6
MS.gene000763.t1 MTR_6g015320 26.056 284 181 8 130 395 137 409 1.05e-14 76.3
MS.gene000763.t1 MTR_7g114520 24.088 411 261 19 38 432 46 421 1.31e-14 75.9
MS.gene000763.t1 MTR_1g040175 26.950 282 182 11 50 324 59 323 1.38e-14 75.9
MS.gene000763.t1 MTR_7g014330 26.942 412 250 17 18 396 20 413 2.27e-14 75.1
MS.gene000763.t1 MTR_1g040320 23.833 407 229 16 44 430 51 396 2.45e-14 75.1
MS.gene000763.t1 MTR_6g015850 24.396 455 282 18 10 429 21 448 3.74e-14 74.7
MS.gene000763.t1 MTR_7g014250 27.132 387 208 19 52 396 59 413 1.40e-13 72.8
MS.gene000763.t1 MTR_7g114490 23.982 442 286 18 25 439 24 442 1.56e-13 72.8
MS.gene000763.t1 MTR_3g071320 26.403 303 183 11 54 334 59 343 1.57e-13 72.4
MS.gene000763.t1 MTR_6g015815 24.816 407 257 14 52 430 67 452 3.25e-13 71.6
MS.gene000763.t1 MTR_3g465930 24.430 307 192 10 36 324 50 334 1.18e-12 70.1
MS.gene000763.t1 MTR_1g040315 27.090 299 187 11 25 309 23 304 1.79e-12 69.3
MS.gene000763.t1 MTR_7g084910 27.562 283 169 11 45 308 53 318 2.94e-12 68.6
MS.gene000763.t1 MTR_3g049140 25.926 189 122 6 226 399 18 203 6.13e-12 65.9
MS.gene000763.t1 MTR_7g014620 25.313 399 229 16 55 422 54 414 1.83e-11 66.2
MS.gene000763.t1 MTR_1g053315 22.169 415 276 19 36 424 54 447 3.22e-11 65.5
MS.gene000763.t1 MTR_8g467590 24.242 462 286 19 6 437 5 432 4.00e-11 65.1
MS.gene000763.t1 MTR_7g014360 26.937 271 169 9 138 396 90 343 5.17e-11 64.7
MS.gene000763.t1 MTR_7g014200 22.961 466 281 17 15 428 19 458 7.80e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000763.t1 AT5G67150 39.474 456 249 12 1 440 1 445 2.25e-98 302
MS.gene000763.t1 AT3G50300 41.458 439 225 14 1 422 1 424 1.83e-93 289
MS.gene000763.t1 AT3G50280 37.639 449 258 10 1 437 1 439 2.88e-90 281
MS.gene000763.t1 AT5G67160 37.838 444 245 10 6 441 12 432 2.34e-89 278
MS.gene000763.t1 AT5G42830 37.500 440 251 10 15 441 21 449 1.82e-86 271
MS.gene000763.t1 AT3G50270 37.389 452 257 11 6 441 7 448 2.46e-86 271
MS.gene000763.t1 AT5G07850 39.775 445 239 13 15 442 22 454 2.61e-84 266
MS.gene000763.t1 AT5G07860 37.908 459 245 12 4 440 8 448 3.71e-84 266
MS.gene000763.t1 AT5G38130 37.615 436 244 12 1 422 21 442 1.65e-81 259
MS.gene000763.t1 AT5G07870 37.029 478 239 16 4 442 8 462 2.01e-75 243
MS.gene000763.t1 AT2G39980 33.680 481 245 13 16 442 21 481 4.53e-69 227
MS.gene000763.t1 AT5G01210 34.255 470 246 12 16 441 19 469 2.18e-66 220
MS.gene000763.t1 AT3G50280 37.304 319 184 6 125 437 1 309 4.99e-62 204
MS.gene000763.t1 AT3G50290 40.146 274 151 6 122 390 4 269 8.25e-53 178
MS.gene000763.t1 AT5G23940 29.978 457 286 14 4 437 3 448 5.35e-50 177
MS.gene000763.t1 AT5G48930 26.244 442 284 13 11 432 9 428 5.68e-31 124
MS.gene000763.t1 AT5G57840 26.889 450 268 21 4 422 3 422 1.04e-22 100
MS.gene000763.t1 AT3G48720 27.094 406 256 15 42 431 44 425 1.41e-22 99.8
MS.gene000763.t1 AT5G41040 25.187 401 262 14 52 439 66 441 1.63e-21 97.1
MS.gene000763.t1 AT5G41040 25.187 401 262 14 52 439 82 457 1.92e-21 96.7
MS.gene000763.t1 AT3G03480 26.290 407 253 16 25 411 33 412 2.64e-19 90.5
MS.gene000763.t1 AT3G29670 22.566 452 308 14 3 430 8 441 2.85e-19 90.1
MS.gene000763.t1 AT3G29590 25.541 462 278 17 3 428 7 438 3.32e-18 87.0
MS.gene000763.t1 AT5G63560 24.552 391 253 15 55 432 61 422 4.19e-18 86.7
MS.gene000763.t1 AT5G23970 25.974 462 267 20 4 437 3 417 9.67e-18 85.5
MS.gene000763.t1 AT5G39080 26.406 409 247 19 52 426 62 450 1.45e-17 85.1
MS.gene000763.t1 AT2G40230 24.888 446 301 17 1 432 1 426 6.34e-17 83.2
MS.gene000763.t1 AT3G29635 24.884 430 272 16 24 422 29 438 1.13e-15 79.3
MS.gene000763.t1 AT5G07080 27.660 282 168 13 56 322 71 331 1.95e-15 78.6
MS.gene000763.t1 AT1G24430 25.416 421 266 18 36 442 40 426 3.39e-15 77.8
MS.gene000763.t1 AT2G25150 24.234 359 243 10 50 396 62 403 5.50e-15 77.0
MS.gene000763.t1 AT1G28680 24.501 351 226 12 58 395 65 389 6.54e-15 77.0
MS.gene000763.t1 AT1G65450 24.918 305 203 11 53 345 70 360 7.19e-15 76.6
MS.gene000763.t1 AT5G16410 24.552 391 255 17 41 414 77 444 1.53e-14 75.9
MS.gene000763.t1 AT3G29680 24.181 397 251 13 52 422 59 431 1.65e-14 75.9
MS.gene000763.t1 AT1G28680 25.714 280 175 9 58 325 65 323 9.44e-14 73.2
MS.gene000763.t1 AT1G78990 25.989 354 227 16 53 393 65 396 1.07e-13 73.2
MS.gene000763.t1 AT3G30280 23.844 411 255 18 54 441 61 436 1.96e-13 72.4
MS.gene000763.t1 AT1G27620 23.929 397 250 18 55 431 58 422 2.14e-13 72.4
MS.gene000763.t1 AT1G32910 24.438 356 235 14 53 393 63 399 2.28e-12 68.9
MS.gene000763.t1 AT5G61160 24.101 278 179 11 135 393 139 403 2.83e-12 68.9
MS.gene000763.t1 AT5G39050 24.611 321 189 12 149 428 160 468 1.95e-11 66.2

Find 92 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCATGGTTTGATGTTTA+TGG 0.113342 4.3:+75891053 MS.gene000763:CDS
CCATTACCAGAAAATTATTT+TGG 0.134239 4.3:+75890822 MS.gene000763:CDS
TTAGCAAGCCTAACAAAATT+TGG 0.155332 4.3:-75890996 None:intergenic
ATAGTTTGATCACTTGATTT+TGG 0.265670 4.3:-75889967 None:intergenic
TTGTCTTCCACATGAGATTC+TGG 0.280245 4.3:+75891184 MS.gene000763:CDS
AAAACTTTGTTTATCAAATA+GGG 0.285623 4.3:+75890605 MS.gene000763:CDS
GATGTTTATGGTAATGATTT+TGG 0.308658 4.3:+75891065 MS.gene000763:CDS
AGTGTCTCAGCCGTTGGATT+TGG 0.349622 4.3:-75890074 None:intergenic
GAAACCAACGCTCTAGTGTT+GGG 0.360883 4.3:-75890488 None:intergenic
TTTAACTCACCTTTGGTTCT+CGG 0.362307 4.3:+75890719 MS.gene000763:CDS
ATTGGCCGTTGAACACCATT+TGG 0.375515 4.3:-75890510 None:intergenic
AATGAAAGTTGGGGAGTTGT+TGG 0.379006 4.3:+75890872 MS.gene000763:CDS
TGGATTTGGTTAGCTTCAAC+TGG 0.379348 4.3:-75890060 None:intergenic
AAATGCAATTATAAGTTGTA+TGG 0.380673 4.3:+75890845 MS.gene000763:CDS
GTGTTTGCCGGAATAGAAAA+AGG 0.382441 4.3:+75891143 MS.gene000763:CDS
ACCTCTCACGTTGCATTAGT+TGG 0.383610 4.3:-75891246 None:intergenic
AAACCATGGAGAACTGCTAA+TGG 0.390568 4.3:-75891042 None:intergenic
GTATGGTTAAAATGAAAGTT+GGG 0.393088 4.3:+75890862 MS.gene000763:CDS
TGTATGGTTAAAATGAAAGT+TGG 0.395906 4.3:+75890861 MS.gene000763:CDS
TGTGGCATTTCATCAATTCA+TGG 0.407032 4.3:+75890421 MS.gene000763:CDS
TGGGATCTCCGGTTTCTACT+TGG 0.411278 4.3:+75890003 MS.gene000763:CDS
GCCGGCAAGAGGTTGGAAAA+AGG 0.411903 4.3:-75890115 None:intergenic
GGACTTGTTACAGTGTTTGC+CGG 0.422586 4.3:+75891131 MS.gene000763:CDS
GAGGTACATAGGTGGGTTCT+AGG 0.423951 4.3:-75890251 None:intergenic
TTTAACCATGTTGTCGTTGA+CGG 0.436978 4.3:+75890390 MS.gene000763:CDS
GACACAACATTCATGAATTG+AGG 0.451357 4.3:-75891224 None:intergenic
GTTGGGGAGTTGTTGGAGGA+AGG 0.453345 4.3:+75890879 MS.gene000763:CDS
CGTAGTGGATATAAAGCAAA+TGG 0.458289 4.3:+75891110 MS.gene000763:CDS
GTATTTAATAGAGTCGTGAT+AGG 0.460162 4.3:+75890777 MS.gene000763:CDS
ATTTGACTCCATGGGATCTC+CGG 0.462323 4.3:+75889992 MS.gene000763:CDS
TGGTGATAAAGAAGACCCTT+TGG 0.469749 4.3:-75890036 None:intergenic
TGAAGAATCACTACGAGTCT+TGG 0.477110 4.3:+75890964 MS.gene000763:CDS
GGGGAGTTGTTGGAGGAAGG+TGG 0.485977 4.3:+75890882 MS.gene000763:CDS
TATCCACTACGAACTGCTAC+AGG 0.486958 4.3:-75891098 None:intergenic
ATTGTTGGCGGTTCAAGTTA+CGG 0.487402 4.3:+75890335 MS.gene000763:CDS
GTGGCATTTCATCAATTCAT+GGG 0.489232 4.3:+75890422 MS.gene000763:CDS
AATAGAGTCGTGATAGGAGT+TGG 0.491643 4.3:+75890783 MS.gene000763:CDS
GGTGATAAAGAAGACCCTTT+GGG 0.492906 4.3:-75890035 None:intergenic
CATGTGGAAGACAAACTTGA+AGG 0.493205 4.3:-75891175 None:intergenic
AGGCACATCAAAACCATTGT+TGG 0.495948 4.3:+75890320 MS.gene000763:CDS
CAACAAAAGAATCACTCTGA+TGG 0.501307 4.3:+75890555 MS.gene000763:CDS
CTATTAAATACTTCTTCTTG+CGG 0.507155 4.3:-75890765 None:intergenic
AAACTATTGATTTGACTCCA+TGG 0.507614 4.3:+75889983 MS.gene000763:CDS
GAAAGTTGGGGAGTTGTTGG+AGG 0.511101 4.3:+75890875 MS.gene000763:CDS
GGAAACCAACGCTCTAGTGT+TGG 0.511281 4.3:-75890489 None:intergenic
CTAGAGCGTTGGTTTCCAAA+TGG 0.513520 4.3:+75890495 MS.gene000763:CDS
AACTATTGATTTGACTCCAT+GGG 0.516810 4.3:+75889984 MS.gene000763:CDS
CACATCAAAACCATTGTTGG+CGG 0.519877 4.3:+75890323 MS.gene000763:CDS
TGTATGTACCAAGTAGAAAC+CGG 0.525201 4.3:-75890011 None:intergenic
ACAAAGAGTGTCTCAGCCGT+TGG 0.530388 4.3:-75890080 None:intergenic
ACATGAGATTCTGGAGGCAA+TGG 0.532230 4.3:+75891193 MS.gene000763:CDS
TGAGACGGCCGGCAAGAGGT+TGG 0.536408 4.3:-75890122 None:intergenic
AAAGTGAAAGGAAATCGTAT+TGG 0.539286 4.3:-75890528 None:intergenic
AACAAAAGAATCACTCTGAT+GGG 0.541294 4.3:+75890556 MS.gene000763:CDS
GAGGAAGGTGGACTTTGTAA+AGG 0.546587 4.3:+75890894 MS.gene000763:CDS
TGCCGGCCGTCTCAAAATAA+CGG 0.548175 4.3:+75890131 MS.gene000763:CDS
ATGAATTGATGAAATGCCAC+AGG 0.555492 4.3:-75890419 None:intergenic
CAAGTTACGGAGCTAGTTGA+CGG 0.556008 4.3:+75890348 MS.gene000763:CDS
ATCAACAATTGGAGGTACAT+AGG 0.556823 4.3:-75890262 None:intergenic
GGTTTCCAAATGGTGTTCAA+CGG 0.558301 4.3:+75890505 MS.gene000763:CDS
GGTAATGGTGGAACTAACCT+TGG 0.563399 4.3:-75890807 None:intergenic
ACACAACATTCATGAATTGA+GGG 0.567594 4.3:-75891223 None:intergenic
GTGATTCTTCAACTTCTCAT+CGG 0.575796 4.3:-75890952 None:intergenic
TTTGGTTAGCTTCAACTGGA+TGG 0.576856 4.3:-75890056 None:intergenic
CTTGGTACATACAACCCAAA+GGG 0.578037 4.3:+75890021 MS.gene000763:CDS
AGTAGAAACCGGAGATCCCA+TGG 0.580798 4.3:-75890000 None:intergenic
TGGTGCCTTGATTGTATCAG+TGG 0.583979 4.3:-75889947 None:intergenic
AAACCAACGCTCTAGTGTTG+GGG 0.590997 4.3:-75890487 None:intergenic
TCTGTCCACTGATACAATCA+AGG 0.594826 4.3:+75889942 MS.gene000763:CDS
AACCAACGCTCTAGTGTTGG+GGG 0.595723 4.3:-75890486 None:intergenic
TATCCATTAGCAGTTCTCCA+TGG 0.597915 4.3:+75891039 MS.gene000763:CDS
GTAACTTGAACCGCCAACAA+TGG 0.600639 4.3:-75890333 None:intergenic
ACAATTGGAGGTACATAGGT+GGG 0.600875 4.3:-75890258 None:intergenic
TATGGTTAAAATGAAAGTTG+GGG 0.603920 4.3:+75890863 MS.gene000763:CDS
ATCAGTGGACAGAACTCGTA+CGG 0.611189 4.3:-75889932 None:intergenic
AACAATTGGAGGTACATAGG+TGG 0.614048 4.3:-75890259 None:intergenic
AACGCATCACCGAGAACCAA+AGG 0.616613 4.3:-75890728 None:intergenic
ACTTTCCGTCAACGACAACA+TGG 0.629179 4.3:-75890395 None:intergenic
ACTTGGTACATACAACCCAA+AGG 0.643558 4.3:+75890020 MS.gene000763:CDS
TCTTCCACATGAGATTCTGG+AGG 0.645425 4.3:+75891187 MS.gene000763:CDS
GCATATAACAGAACACGAGA+CGG 0.649984 4.3:-75890169 None:intergenic
TTACCATAAACATCAAACCA+TGG 0.659822 4.3:-75891056 None:intergenic
TCTGTTATATGCAACAATGC+AGG 0.665996 4.3:+75890180 MS.gene000763:CDS
AAGCCTGTAGCAGTTCGTAG+TGG 0.668630 4.3:+75891095 MS.gene000763:CDS
TCGTGATAGGAGTTGGACCA+AGG 0.676348 4.3:+75890790 MS.gene000763:CDS
TTGAAGCTAACCAAATCCAA+CGG 0.678953 4.3:+75890064 MS.gene000763:CDS
ATTGCCTCCAGAATCTCATG+TGG 0.679505 4.3:-75891191 None:intergenic
ACTGAGGTTAGAAGCTACGA+AGG 0.680362 4.3:+75890300 MS.gene000763:CDS
TGAATTGATGAAATGCCACA+GGG 0.685982 4.3:-75890418 None:intergenic
AAGTATTCTCTGCTGCAGCG+TGG 0.693165 4.3:-75890215 None:intergenic
ACCAACGCTCTAGTGTTGGG+GGG 0.706711 4.3:-75890485 None:intergenic
TCGTTGACGGAAAGTCCCTG+TGG 0.745350 4.3:+75890403 MS.gene000763:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAACTTTGTTTATCAAAT+AGG + chr4.3:75890604-75890623 MS.gene000763:CDS 15.0%
!!! AAAACTTTGTTTATCAAATA+GGG + chr4.3:75890605-75890624 MS.gene000763:CDS 15.0%
!! AAATGCAATTATAAGTTGTA+TGG + chr4.3:75890845-75890864 MS.gene000763:CDS 20.0%
!! TTAAAAATCAATGAAGAGAT+TGG + chr4.3:75890666-75890685 MS.gene000763:CDS 20.0%
!!! TAAGAAATCCAAATTTTGTT+AGG + chr4.3:75890988-75891007 MS.gene000763:CDS 20.0%
! AAAAATGAATCAACAATTGG+AGG - chr4.3:75890273-75890292 None:intergenic 25.0%
! CTATTAAATACTTCTTCTTG+CGG - chr4.3:75890768-75890787 None:intergenic 25.0%
! GTATGGTTAAAATGAAAGTT+GGG + chr4.3:75890862-75890881 MS.gene000763:CDS 25.0%
! TATGGTTAAAATGAAAGTTG+GGG + chr4.3:75890863-75890882 MS.gene000763:CDS 25.0%
! TGTATGGTTAAAATGAAAGT+TGG + chr4.3:75890861-75890880 MS.gene000763:CDS 25.0%
!! ATAGTTTGATCACTTGATTT+TGG - chr4.3:75889970-75889989 None:intergenic 25.0%
!! CCATTACCAGAAAATTATTT+TGG + chr4.3:75890822-75890841 MS.gene000763:CDS 25.0%
!! GCATTTCCAAAATAATTTTC+TGG - chr4.3:75890831-75890850 None:intergenic 25.0%
!! TATACTACCTTTTTCTATTC+CGG - chr4.3:75891153-75891172 None:intergenic 25.0%
!!! AAATAATTTTCTGGTAATGG+TGG - chr4.3:75890822-75890841 None:intergenic 25.0%
!!! CCAAAATAATTTTCTGGTAA+TGG - chr4.3:75890825-75890844 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr4.3:75891065-75891084 MS.gene000763:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG + chr4.3:75891070-75891089 MS.gene000763:CDS 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG + chr4.3:75891069-75891088 MS.gene000763:CDS 25.0%
AAAGTGAAAGGAAATCGTAT+TGG - chr4.3:75890531-75890550 None:intergenic 30.0%
AACAAAAGAATCACTCTGAT+GGG + chr4.3:75890556-75890575 MS.gene000763:CDS 30.0%
ACACAACATTCATGAATTGA+GGG - chr4.3:75891226-75891245 None:intergenic 30.0%
GCGAAAAATGAATCAACAAT+TGG - chr4.3:75890276-75890295 None:intergenic 30.0%
GTATTTAATAGAGTCGTGAT+AGG + chr4.3:75890777-75890796 MS.gene000763:CDS 30.0%
TTACCATAAACATCAAACCA+TGG - chr4.3:75891059-75891078 None:intergenic 30.0%
TTAGCAAGCCTAACAAAATT+TGG - chr4.3:75890999-75891018 None:intergenic 30.0%
!! AAACTATTGATTTGACTCCA+TGG + chr4.3:75889983-75890002 MS.gene000763:CDS 30.0%
!! AACTATTGATTTGACTCCAT+GGG + chr4.3:75889984-75890003 MS.gene000763:CDS 30.0%
!! TATGGTAATGATTTTGGATG+GGG + chr4.3:75891071-75891090 MS.gene000763:CDS 30.0%
!! TTCATTTTTCGCTTTAACTG+AGG + chr4.3:75890284-75890303 MS.gene000763:CDS 30.0%
!! TTTTGTTGCTTCAAAGTGAA+AGG - chr4.3:75890543-75890562 None:intergenic 30.0%
ATGAATTGATGAAATGCCAC+AGG - chr4.3:75890422-75890441 None:intergenic 35.0%
CAACAAAAGAATCACTCTGA+TGG + chr4.3:75890555-75890574 MS.gene000763:CDS 35.0%
GACACAACATTCATGAATTG+AGG - chr4.3:75891227-75891246 None:intergenic 35.0%
GTGATTCTTCAACTTCTCAT+CGG - chr4.3:75890955-75890974 None:intergenic 35.0%
GTGGCATTTCATCAATTCAT+GGG + chr4.3:75890422-75890441 MS.gene000763:CDS 35.0%
TCTCCATGGTTTGATGTTTA+TGG + chr4.3:75891053-75891072 MS.gene000763:CDS 35.0%
TCTGTTATATGCAACAATGC+AGG + chr4.3:75890180-75890199 MS.gene000763:CDS 35.0%
TGAATTGATGAAATGCCACA+GGG - chr4.3:75890421-75890440 None:intergenic 35.0%
TGTATGTACCAAGTAGAAAC+CGG - chr4.3:75890014-75890033 None:intergenic 35.0%
TGTGGCATTTCATCAATTCA+TGG + chr4.3:75890421-75890440 MS.gene000763:CDS 35.0%
TTGAAGCTAACCAAATCCAA+CGG + chr4.3:75890064-75890083 MS.gene000763:CDS 35.0%
TTTAACCATGTTGTCGTTGA+CGG + chr4.3:75890390-75890409 MS.gene000763:CDS 35.0%
! ATCAACAATTGGAGGTACAT+AGG - chr4.3:75890265-75890284 None:intergenic 35.0%
! CTAGTTGACGGTATTTTCAT+TGG + chr4.3:75890360-75890379 MS.gene000763:CDS 35.0%
!! AAGCACTTTTAACTCACCTT+TGG + chr4.3:75890712-75890731 MS.gene000763:CDS 35.0%
!! CGTAGTGGATATAAAGCAAA+TGG + chr4.3:75891110-75891129 MS.gene000763:CDS 35.0%
!! TTTAACTCACCTTTGGTTCT+CGG + chr4.3:75890719-75890738 MS.gene000763:CDS 35.0%
!!! TGTGTTCCGTTATTTTGAGA+CGG - chr4.3:75890140-75890159 None:intergenic 35.0%
!!! TTTTGTTAGGCTTGCTAACA+TGG + chr4.3:75891001-75891020 MS.gene000763:CDS 35.0%
AAACCATGGAGAACTGCTAA+TGG - chr4.3:75891045-75891064 None:intergenic 40.0%
AAGAGGTTGGAAAAAGGCAA+GGG - chr4.3:75890112-75890131 None:intergenic 40.0%
AATAGAGTCGTGATAGGAGT+TGG + chr4.3:75890783-75890802 MS.gene000763:CDS 40.0%
AATGAAAGTTGGGGAGTTGT+TGG + chr4.3:75890872-75890891 MS.gene000763:CDS 40.0%
ACTTGGTACATACAACCCAA+AGG + chr4.3:75890020-75890039 MS.gene000763:CDS 40.0%
AGGCACATCAAAACCATTGT+TGG + chr4.3:75890320-75890339 MS.gene000763:CDS 40.0%
CACATCAAAACCATTGTTGG+CGG + chr4.3:75890323-75890342 MS.gene000763:CDS 40.0%
CATGTGGAAGACAAACTTGA+AGG - chr4.3:75891178-75891197 None:intergenic 40.0%
CTTGGTACATACAACCCAAA+GGG + chr4.3:75890021-75890040 MS.gene000763:CDS 40.0%
GCATATAACAGAACACGAGA+CGG - chr4.3:75890172-75890191 None:intergenic 40.0%
GGTGATAAAGAAGACCCTTT+GGG - chr4.3:75890038-75890057 None:intergenic 40.0%
GGTTTCCAAATGGTGTTCAA+CGG + chr4.3:75890505-75890524 MS.gene000763:CDS 40.0%
GTGTTTGCCGGAATAGAAAA+AGG + chr4.3:75891143-75891162 MS.gene000763:CDS 40.0%
TATCCATTAGCAGTTCTCCA+TGG + chr4.3:75891039-75891058 MS.gene000763:CDS 40.0%
TCTGTCCACTGATACAATCA+AGG + chr4.3:75889942-75889961 MS.gene000763:CDS 40.0%
TGAAGAATCACTACGAGTCT+TGG + chr4.3:75890964-75890983 MS.gene000763:CDS 40.0%
TGGTGATAAAGAAGACCCTT+TGG - chr4.3:75890039-75890058 None:intergenic 40.0%
TTGTCTTCCACATGAGATTC+TGG + chr4.3:75891184-75891203 MS.gene000763:CDS 40.0%
! AACAATTGGAGGTACATAGG+TGG - chr4.3:75890262-75890281 None:intergenic 40.0%
! ACAATTGGAGGTACATAGGT+GGG - chr4.3:75890261-75890280 None:intergenic 40.0%
! ATTGTTGGCGGTTCAAGTTA+CGG + chr4.3:75890335-75890354 MS.gene000763:CDS 40.0%
!! TGGATTTGGTTAGCTTCAAC+TGG - chr4.3:75890063-75890082 None:intergenic 40.0%
!! TTTGGTTAGCTTCAACTGGA+TGG - chr4.3:75890059-75890078 None:intergenic 40.0%
AAACCAACGCTCTAGTGTTG+GGG - chr4.3:75890490-75890509 None:intergenic 45.0%
ACTGAGGTTAGAAGCTACGA+AGG + chr4.3:75890300-75890319 MS.gene000763:CDS 45.0%
ACTTTCCGTCAACGACAACA+TGG - chr4.3:75890398-75890417 None:intergenic 45.0%
ATCAGTGGACAGAACTCGTA+CGG - chr4.3:75889935-75889954 None:intergenic 45.0%
ATTGCCTCCAGAATCTCATG+TGG - chr4.3:75891194-75891213 None:intergenic 45.0%
ATTGGCCGTTGAACACCATT+TGG - chr4.3:75890513-75890532 None:intergenic 45.0%
ATTTGACTCCATGGGATCTC+CGG + chr4.3:75889992-75890011 MS.gene000763:CDS 45.0%
CAAGAGGTTGGAAAAAGGCA+AGG - chr4.3:75890113-75890132 None:intergenic 45.0%
CAAGTTACGGAGCTAGTTGA+CGG + chr4.3:75890348-75890367 MS.gene000763:CDS 45.0%
GAAACCAACGCTCTAGTGTT+GGG - chr4.3:75890491-75890510 None:intergenic 45.0%
GTAACTTGAACCGCCAACAA+TGG - chr4.3:75890336-75890355 None:intergenic 45.0%
TATCCACTACGAACTGCTAC+AGG - chr4.3:75891101-75891120 None:intergenic 45.0%
TCTTCCACATGAGATTCTGG+AGG + chr4.3:75891187-75891206 MS.gene000763:CDS 45.0%
TGGTGCCTTGATTGTATCAG+TGG - chr4.3:75889950-75889969 None:intergenic 45.0%
! ACATGAGATTCTGGAGGCAA+TGG + chr4.3:75891193-75891212 MS.gene000763:CDS 45.0%
! GAGGAAGGTGGACTTTGTAA+AGG + chr4.3:75890894-75890913 MS.gene000763:CDS 45.0%
! GGTAATGGTGGAACTAACCT+TGG - chr4.3:75890810-75890829 None:intergenic 45.0%
!! CTAGAGCGTTGGTTTCCAAA+TGG + chr4.3:75890495-75890514 MS.gene000763:CDS 45.0%
!! GGACTTGTTACAGTGTTTGC+CGG + chr4.3:75891131-75891150 MS.gene000763:CDS 45.0%
!!! TTCCGTTATTTTGAGACGGC+CGG - chr4.3:75890136-75890155 None:intergenic 45.0%
AACGCATCACCGAGAACCAA+AGG - chr4.3:75890731-75890750 None:intergenic 50.0%
AAGCCTGTAGCAGTTCGTAG+TGG + chr4.3:75891095-75891114 MS.gene000763:CDS 50.0%
AAGTATTCTCTGCTGCAGCG+TGG - chr4.3:75890218-75890237 None:intergenic 50.0%
AGTAGAAACCGGAGATCCCA+TGG - chr4.3:75890003-75890022 None:intergenic 50.0%
AGTGTCTCAGCCGTTGGATT+TGG - chr4.3:75890077-75890096 None:intergenic 50.0%
GAAAGTTGGGGAGTTGTTGG+AGG + chr4.3:75890875-75890894 MS.gene000763:CDS 50.0%
GGAAACCAACGCTCTAGTGT+TGG - chr4.3:75890492-75890511 None:intergenic 50.0%
TCGTGATAGGAGTTGGACCA+AGG + chr4.3:75890790-75890809 MS.gene000763:CDS 50.0%
TGCCGGCCGTCTCAAAATAA+CGG + chr4.3:75890131-75890150 MS.gene000763:CDS 50.0%
TGGGATCTCCGGTTTCTACT+TGG + chr4.3:75890003-75890022 MS.gene000763:CDS 50.0%
TTGGAAAAAGGCAAGGGCAG+AGG - chr4.3:75890106-75890125 None:intergenic 50.0%
! AACCAACGCTCTAGTGTTGG+GGG - chr4.3:75890489-75890508 None:intergenic 50.0%
! GAGGTACATAGGTGGGTTCT+AGG - chr4.3:75890254-75890273 None:intergenic 50.0%
! GCCTTTTTCCAACCTCTTGC+CGG + chr4.3:75890114-75890133 MS.gene000763:CDS 50.0%
!! ACAAAGAGTGTCTCAGCCGT+TGG - chr4.3:75890083-75890102 None:intergenic 50.0%
GCCGGCAAGAGGTTGGAAAA+AGG - chr4.3:75890118-75890137 None:intergenic 55.0%
GTTGGGGAGTTGTTGGAGGA+AGG + chr4.3:75890879-75890898 MS.gene000763:CDS 55.0%
TCGTTGACGGAAAGTCCCTG+TGG + chr4.3:75890403-75890422 MS.gene000763:CDS 55.0%
! ACCAACGCTCTAGTGTTGGG+GGG - chr4.3:75890488-75890507 None:intergenic 55.0%
!!! ATTTTGAGACGGCCGGCAAG+AGG - chr4.3:75890129-75890148 None:intergenic 55.0%
ACCCCCCAACACTAGAGCGT+TGG + chr4.3:75890484-75890503 MS.gene000763:CDS 60.0%
GGGGAGTTGTTGGAGGAAGG+TGG + chr4.3:75890882-75890901 MS.gene000763:CDS 60.0%
TGAGACGGCCGGCAAGAGGT+TGG - chr4.3:75890125-75890144 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 75889925 75891253 75889925 ID=MS.gene000763
chr4.3 mRNA 75889925 75891253 75889925 ID=MS.gene000763.t1;Parent=MS.gene000763
chr4.3 exon 75889925 75891253 75889925 ID=MS.gene000763.t1.exon1;Parent=MS.gene000763.t1
chr4.3 CDS 75889925 75891253 75889925 ID=cds.MS.gene000763.t1;Parent=MS.gene000763.t1
Gene Sequence

>MS.gene000763

ATGGGAACCGTACGAGTTCTGTCCACTGATACAATCAAGGCACCAAAATCAAGTGATCAAACTATTGATTTGACTCCATGGGATCTCCGGTTTCTACTTGGTACATACAACCCAAAGGGTCTTCTTTATCACCATCCAGTTGAAGCTAACCAAATCCAACGGCTGAGACACTCTTTGTCCTCTGCCCTTGCCTTTTTCCAACCTCTTGCCGGCCGTCTCAAAATAACGGAACACAATGACAACACCGTCTCGTGTTCTGTTATATGCAACAATGCAGGTGTACTCTTTATCCACGCTGCAGCAGAGAATACTTGTGTTGCTGACATCCTAGAACCCACCTATGTACCTCCAATTGTTGATTCATTTTTCGCTTTAACTGAGGTTAGAAGCTACGAAGGCACATCAAAACCATTGTTGGCGGTTCAAGTTACGGAGCTAGTTGACGGTATTTTCATTGGTTGTTCATTTAACCATGTTGTCGTTGACGGAAAGTCCCTGTGGCATTTCATCAATTCATGGGCTGAAATTTCACGTAGTTGTTGTCGTCATCAAATATCTAACCCCCCAACACTAGAGCGTTGGTTTCCAAATGGTGTTCAACGGCCAATACGATTTCCTTTCACTTTGAAGCAACAAAAGAATCACTCTGATGGGTTGAGTTTTTCGTCGTTTAATGATGAAAAACTTTGTTTATCAAATAGGGTATTTCATTTCACAATAGAGAAAATTGTACAGCTGAAATTAAAAATCAATGAAGAGATTGGTGTCATCAAAATATCTTCTCTACAAGCACTTTTAACTCACCTTTGGTTCTCGGTGATGCGTTTCAAACAATTTGATCCGCAAGAAGAAGTATTTAATAGAGTCGTGATAGGAGTTGGACCAAGGTTAGTTCCACCATTACCAGAAAATTATTTTGGAAATGCAATTATAAGTTGTATGGTTAAAATGAAAGTTGGGGAGTTGTTGGAGGAAGGTGGACTTTGTAAAGGTGCTTGCGAGATGAACAAGTTAATTGCTTCACACACCGATGAGAAGTTGAAGAATCACTACGAGTCTTGGTTAAGAAATCCAAATTTTGTTAGGCTTGCTAACATGGTAAAAAATAATTTCATATCCATTAGCAGTTCTCCATGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAGCCTGTAGCAGTTCGTAGTGGATATAAAGCAAATGGACTTGTTACAGTGTTTGCCGGAATAGAAAAAGGTAGTATAGACCTTCAAGTTTGTCTTCCACATGAGATTCTGGAGGCAATGGAAAATGACCCTCAATTCATGAATGTTGTGTCCAACTAA
Protein sequence

>MS.gene000763.t1

MGTVRVLSTDTIKAPKSSDQTIDLTPWDLRFLLGTYNPKGLLYHHPVEANQIQRLRHSLSSALAFFQPLAGRLKITEHNDNTVSCSVICNNAGVLFIHAAAENTCVADILEPTYVPPIVDSFFALTEVRSYEGTSKPLLAVQVTELVDGIFIGCSFNHVVVDGKSLWHFINSWAEISRSCCRHQISNPPTLERWFPNGVQRPIRFPFTLKQQKNHSDGLSFSSFNDEKLCLSNRVFHFTIEKIVQLKLKINEEIGVIKISSLQALLTHLWFSVMRFKQFDPQEEVFNRVVIGVGPRLVPPLPENYFGNAIISCMVKMKVGELLEEGGLCKGACEMNKLIASHTDEKLKNHYESWLRNPNFVRLANMVKNNFISISSSPWFDVYGNDFGWGKPVAVRSGYKANGLVTVFAGIEKGSIDLQVCLPHEILEAMENDPQFMNVVSN