AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000764

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000764.t1 MTR_4g022825 92.955 440 31 0 1 440 1 440 0.0 851
MS.gene000764.t1 MTR_4g022720 73.696 441 109 2 1 440 1 435 0.0 678
MS.gene000764.t1 MTR_4g022800 72.624 442 119 2 1 440 1 442 0.0 676
MS.gene000764.t1 MTR_4g022670 72.955 440 118 1 1 439 1 440 0.0 672
MS.gene000764.t1 MTR_7g026860 72.562 441 117 2 1 440 1 438 0.0 669
MS.gene000764.t1 MTR_3g088420 66.818 440 139 1 1 440 1 433 0.0 610
MS.gene000764.t1 MTR_3g026310 64.877 447 149 3 1 439 1 447 0.0 582
MS.gene000764.t1 MTR_4g022760 64.399 441 112 2 1 440 1 397 0.0 573
MS.gene000764.t1 MTR_8g041770 58.667 450 165 6 1 439 1 440 0.0 531
MS.gene000764.t1 MTR_0190s0020 56.984 451 174 7 1 440 1 442 0.0 514
MS.gene000764.t1 MTR_4g094835 54.725 455 174 9 1 436 1 442 7.56e-169 482
MS.gene000764.t1 MTR_4g094842 56.044 455 174 8 1 438 1 446 8.81e-168 479
MS.gene000764.t1 MTR_4g094838 52.203 454 166 11 6 436 3 428 1.44e-153 442
MS.gene000764.t1 MTR_4g094828 50.327 459 198 12 1 439 1 449 5.18e-145 421
MS.gene000764.t1 MTR_4g094832 55.759 382 143 6 69 436 57 426 4.12e-144 418
MS.gene000764.t1 MTR_6g016815 52.500 440 136 7 1 440 15 381 7.24e-142 410
MS.gene000764.t1 MTR_8g079710 50.437 458 194 12 1 440 1 443 1.83e-141 412
MS.gene000764.t1 MTR_4g022290 50.109 459 190 13 1 437 1 442 6.47e-138 403
MS.gene000764.t1 MTR_3g101900 38.739 444 244 12 12 440 18 448 9.13e-92 285
MS.gene000764.t1 MTR_3g102020 38.409 440 243 10 15 437 20 448 3.60e-88 276
MS.gene000764.t1 MTR_8g040760 62.857 210 72 2 1 204 1 210 1.81e-87 266
MS.gene000764.t1 MTR_3g101930 37.615 436 241 10 24 439 30 454 4.45e-86 270
MS.gene000764.t1 MTR_3g101970 37.004 454 247 11 3 439 5 436 5.58e-86 270
MS.gene000764.t1 MTR_3g101960 37.757 437 245 10 15 435 20 445 4.30e-85 268
MS.gene000764.t1 MTR_8g040780 59.722 216 83 2 224 435 12 227 1.03e-84 260
MS.gene000764.t1 MTR_3g102010 37.644 433 243 10 24 440 30 451 2.12e-83 263
MS.gene000764.t1 MTR_7g026850 73.585 159 41 1 29 186 12 170 1.35e-81 249
MS.gene000764.t1 MTR_7g026770 59.474 190 48 2 141 329 132 293 1.80e-70 225
MS.gene000764.t1 MTR_3g070170 33.043 460 251 11 16 436 25 466 3.30e-62 209
MS.gene000764.t1 MTR_4g015180 32.990 485 247 11 16 439 19 486 4.20e-62 209
MS.gene000764.t1 MTR_5g081960 31.601 481 252 10 16 436 19 482 6.57e-61 206
MS.gene000764.t1 MTR_6g045553 31.416 452 272 10 16 436 27 471 7.19e-55 189
MS.gene000764.t1 MTR_4g094825 49.198 187 88 4 17 198 85 269 4.22e-53 182
MS.gene000764.t1 MTR_4g094825 57.823 147 49 2 292 436 252 387 1.03e-48 171
MS.gene000764.t1 MTR_3g102030 34.713 314 164 7 87 396 44 320 8.11e-51 174
MS.gene000764.t1 MTR_6g034495 61.194 134 42 3 57 180 31 164 2.30e-48 163
MS.gene000764.t1 MTR_6g034485 56.061 132 50 2 188 319 2 125 5.84e-44 150
MS.gene000764.t1 MTR_7g026800 49.718 177 58 4 205 379 13 160 8.18e-39 139
MS.gene000764.t1 MTR_4g022780 49.133 173 57 4 211 381 20 163 5.06e-38 136
MS.gene000764.t1 MTR_7g026830 71.429 84 24 0 82 165 14 97 1.33e-36 130
MS.gene000764.t1 MTR_7g026780 56.364 110 24 2 331 440 26 111 6.28e-31 115
MS.gene000764.t1 MTR_8g096530 30.028 353 214 12 49 391 53 382 1.89e-29 119
MS.gene000764.t1 MTR_4g007540 29.888 358 217 15 55 398 56 393 4.13e-28 115
MS.gene000764.t1 MTR_8g075610 26.933 401 233 16 55 429 47 413 4.59e-28 115
MS.gene000764.t1 MTR_3g098980 27.441 379 237 13 55 421 66 418 8.41e-27 112
MS.gene000764.t1 MTR_2g064495 27.604 384 241 15 55 422 56 418 1.49e-26 111
MS.gene000764.t1 MTR_2g105330 27.344 384 242 15 55 422 56 418 1.63e-26 111
MS.gene000764.t1 MTR_3g026950 26.042 384 211 16 52 392 51 404 2.41e-22 99.4
MS.gene000764.t1 MTR_1g081660 26.303 403 226 16 24 392 31 396 7.32e-22 97.8
MS.gene000764.t1 MTR_3g071320 25.115 434 291 14 17 436 19 432 6.28e-21 95.1
MS.gene000764.t1 MTR_2g089650 24.638 414 265 18 38 417 43 443 1.46e-20 94.0
MS.gene000764.t1 MTR_4g013050 26.016 369 188 12 53 392 51 363 4.78e-20 92.0
MS.gene000764.t1 MTR_7g026870 74.074 54 14 0 297 350 98 151 9.15e-20 85.9
MS.gene000764.t1 MTR_3g086020 26.329 395 242 16 55 440 61 415 3.12e-19 89.7
MS.gene000764.t1 MTR_7g015860 24.029 412 248 14 22 392 26 413 5.13e-19 89.4
MS.gene000764.t1 MTR_3g049150 25.442 283 168 9 138 391 131 399 8.34e-19 89.0
MS.gene000764.t1 MTR_7g114520 25.556 360 237 15 44 392 53 392 1.16e-18 88.2
MS.gene000764.t1 MTR_7g114550 23.702 443 281 18 25 436 24 440 3.46e-18 87.0
MS.gene000764.t1 MTR_7g026880 68.627 51 16 0 389 439 2 52 3.99e-18 78.2
MS.gene000764.t1 MTR_3g112180 25.123 406 243 17 49 427 55 426 8.22e-18 85.9
MS.gene000764.t1 MTR_5g076700 24.533 428 269 18 25 422 24 427 8.41e-18 85.9
MS.gene000764.t1 MTR_4g097090 24.079 407 273 13 55 439 65 457 1.24e-17 85.1
MS.gene000764.t1 MTR_1g053315 23.810 420 257 17 33 422 60 446 2.98e-17 84.0
MS.gene000764.t1 MTR_2g089765 25.000 412 241 14 26 391 45 434 4.96e-17 83.6
MS.gene000764.t1 MTR_7g014160 25.945 397 254 12 55 421 69 455 5.02e-17 83.6
MS.gene000764.t1 MTR_7g114470 25.000 432 263 18 25 422 25 429 7.36e-17 82.8
MS.gene000764.t1 MTR_7g027175 24.870 386 253 14 55 427 68 429 1.90e-16 81.6
MS.gene000764.t1 MTR_6g015320 25.559 313 201 12 137 427 142 444 2.14e-16 81.3
MS.gene000764.t1 MTR_8g012750 24.079 407 264 12 22 391 24 422 2.59e-16 81.3
MS.gene000764.t1 MTR_2g089735 25.708 424 255 17 41 424 43 446 3.94e-16 80.5
MS.gene000764.t1 MTR_7g014200 23.025 443 292 13 22 427 28 458 5.93e-16 80.1
MS.gene000764.t1 MTR_6g015315 24.601 313 211 10 137 430 139 445 7.80e-16 79.7
MS.gene000764.t1 MTR_2g089670 23.981 417 242 16 21 391 42 429 8.27e-16 79.7
MS.gene000764.t1 MTR_6g015830 25.057 439 290 13 17 429 27 452 1.08e-15 79.3
MS.gene000764.t1 MTR_4g055170 27.124 306 194 11 55 350 69 355 1.16e-15 79.3
MS.gene000764.t1 MTR_7g084940 26.165 279 185 8 53 323 67 332 1.65e-15 78.6
MS.gene000764.t1 MTR_6g015815 21.719 442 291 14 22 429 32 452 2.69e-15 78.2
MS.gene000764.t1 MTR_6g015850 23.611 432 290 12 22 428 32 448 4.52e-15 77.4
MS.gene000764.t1 MTR_1g040195 24.645 422 260 18 25 427 23 405 6.38e-15 76.6
MS.gene000764.t1 MTR_5g084870 23.843 432 268 16 5 413 4 397 6.42e-15 76.6
MS.gene000764.t1 MTR_7g014610 25.553 407 246 16 55 421 68 457 1.47e-14 75.9
MS.gene000764.t1 MTR_6g053810 26.429 280 181 11 55 323 71 336 1.50e-14 75.9
MS.gene000764.t1 MTR_7g085930 26.523 279 186 6 43 308 47 319 1.70e-14 75.5
MS.gene000764.t1 MTR_7g084910 27.037 270 163 9 53 307 68 318 2.59e-14 75.1
MS.gene000764.t1 MTR_8g079950 25.000 372 236 15 41 396 42 386 5.17e-14 73.9
MS.gene000764.t1 MTR_1g040215 22.951 427 267 17 8 408 13 403 9.91e-14 73.2
MS.gene000764.t1 MTR_7g086000 26.265 415 256 18 58 439 63 460 2.56e-13 72.0
MS.gene000764.t1 MTR_7g085990 25.763 295 168 10 43 308 43 315 9.88e-13 70.1
MS.gene000764.t1 MTR_5g090810 24.130 431 263 19 2 392 6 412 2.71e-12 68.9
MS.gene000764.t1 MTR_3g465990 23.913 276 191 8 55 323 68 331 1.06e-11 67.0
MS.gene000764.t1 MTR_7g013850 21.831 426 263 16 52 427 65 470 1.48e-11 66.6
MS.gene000764.t1 MTR_5g090830 24.362 431 262 19 2 392 6 412 1.51e-11 66.6
MS.gene000764.t1 MTR_5g090880 24.798 371 225 17 55 393 67 415 6.09e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000764.t1 AT5G67150 43.349 436 224 11 16 439 21 445 9.60e-112 336
MS.gene000764.t1 AT3G50300 42.069 435 227 11 1 421 1 424 1.99e-99 305
MS.gene000764.t1 AT3G50280 40.371 431 239 8 16 437 19 440 8.60e-98 300
MS.gene000764.t1 AT5G67160 38.657 432 236 10 17 440 22 432 2.20e-92 286
MS.gene000764.t1 AT3G50270 37.748 453 260 10 2 440 4 448 3.21e-90 281
MS.gene000764.t1 AT5G42830 37.358 439 250 12 16 440 22 449 2.36e-84 266
MS.gene000764.t1 AT5G38130 39.379 419 226 12 16 421 39 442 6.40e-84 265
MS.gene000764.t1 AT5G07860 37.701 435 235 11 24 439 31 448 1.03e-80 256
MS.gene000764.t1 AT5G07870 37.037 432 228 11 38 440 45 461 4.43e-74 239
MS.gene000764.t1 AT5G07850 36.878 442 235 13 24 440 31 453 1.08e-73 238
MS.gene000764.t1 AT2G39980 33.124 477 248 10 16 439 21 479 3.28e-69 228
MS.gene000764.t1 AT3G50280 38.871 319 180 5 125 437 1 310 6.64e-66 214
MS.gene000764.t1 AT5G01210 34.115 469 247 10 16 440 19 469 4.97e-65 216
MS.gene000764.t1 AT5G23940 33.043 460 267 13 1 436 6 448 1.48e-64 215
MS.gene000764.t1 AT3G50290 40.876 274 148 7 122 389 4 269 3.01e-50 172
MS.gene000764.t1 AT5G48930 28.997 369 210 12 53 398 54 393 9.94e-29 117
MS.gene000764.t1 AT3G29670 24.944 449 290 16 3 421 2 433 1.81e-24 105
MS.gene000764.t1 AT3G48720 27.198 364 230 12 42 392 44 385 4.40e-24 104
MS.gene000764.t1 AT3G29590 26.174 447 297 17 5 431 9 442 2.12e-23 102
MS.gene000764.t1 AT2G19070 24.454 458 275 19 27 438 19 451 6.44e-22 98.2
MS.gene000764.t1 AT5G41040 27.451 357 220 16 50 392 64 395 5.63e-21 95.1
MS.gene000764.t1 AT5G41040 27.451 357 220 16 50 392 80 411 6.48e-21 95.1
MS.gene000764.t1 AT5G57840 26.877 413 241 17 41 421 39 422 1.45e-19 90.9
MS.gene000764.t1 AT3G29680 25.693 397 250 14 49 421 56 431 2.73e-19 90.1
MS.gene000764.t1 AT3G03480 26.376 436 259 19 25 427 33 439 5.52e-19 89.4
MS.gene000764.t1 AT3G29635 25.115 434 269 17 22 421 27 438 6.01e-19 89.4
MS.gene000764.t1 AT2G40230 24.719 445 302 18 1 431 1 426 1.99e-18 87.4
MS.gene000764.t1 AT5G39050 24.409 381 228 13 66 414 90 442 3.27e-18 87.0
MS.gene000764.t1 AT1G24430 23.963 434 265 17 26 440 38 425 4.55e-18 86.7
MS.gene000764.t1 AT3G26040 24.331 411 258 16 49 440 53 429 5.27e-18 86.3
MS.gene000764.t1 AT5G63560 26.478 389 239 16 50 422 56 413 5.54e-18 86.3
MS.gene000764.t1 AT5G07080 27.365 296 182 13 42 321 53 331 1.89e-16 81.6
MS.gene000764.t1 AT1G28680 25.000 404 241 17 58 440 65 427 4.81e-16 80.5
MS.gene000764.t1 AT1G03390 25.490 408 255 19 42 422 59 444 7.77e-16 79.7
MS.gene000764.t1 AT5G39090 26.838 272 172 11 135 391 140 399 3.66e-15 77.8
MS.gene000764.t1 AT5G61160 20.802 399 270 13 22 392 23 403 5.45e-14 73.9
MS.gene000764.t1 AT3G30280 23.861 373 214 15 54 398 61 391 5.88e-14 73.9
MS.gene000764.t1 AT1G28680 24.752 303 195 11 58 348 65 346 9.26e-14 73.2
MS.gene000764.t1 AT5G23970 25.612 449 284 19 4 436 3 417 5.75e-13 70.9
MS.gene000764.t1 AT1G78990 25.135 370 236 15 38 392 53 396 1.01e-12 70.1
MS.gene000764.t1 AT2G23510 24.679 389 251 19 55 422 69 436 2.03e-12 69.3
MS.gene000764.t1 AT2G25150 25.414 362 234 14 50 395 62 403 3.06e-12 68.6
MS.gene000764.t1 AT5G16410 22.084 403 276 14 28 413 63 444 3.26e-12 68.6
MS.gene000764.t1 AT3G47170 25.503 298 185 11 44 321 64 344 4.68e-12 68.2
MS.gene000764.t1 AT1G32910 24.932 369 216 17 53 392 63 399 7.56e-12 67.4
MS.gene000764.t1 AT1G65450 24.028 283 193 11 53 324 70 341 8.96e-12 67.0
MS.gene000764.t1 AT4G15390 22.525 404 258 15 10 394 24 391 1.68e-11 66.2
MS.gene000764.t1 AT4G15390 22.525 404 258 15 10 394 24 391 1.68e-11 66.2

Find 83 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCATGGTTTGATGTTTA+TGG 0.113342 4.3:-75885214 MS.gene000764:CDS
GTGTCTTCCTTACAAGATTT+TGG 0.202537 4.3:-75885083 MS.gene000764:CDS
CCATTGCCAGAAGATTATTT+TGG 0.248348 4.3:-75885445 MS.gene000764:CDS
GATGTTTATGGTAATGATTT+TGG 0.308658 4.3:-75885202 MS.gene000764:CDS
TGGTTGGATAAAGTAGAAAT+TGG 0.327409 4.3:+75886253 None:intergenic
GCGCTTAATACGTTCATAAT+AGG 0.328958 4.3:-75885490 MS.gene000764:CDS
TGATCATGAATTCATGGATA+TGG 0.332225 4.3:-75885047 MS.gene000764:CDS
AATGTCTTAGCCGTTGAATT+TGG 0.349561 4.3:+75886190 None:intergenic
TTCTTGTGGATCCAGATGTT+TGG 0.352700 4.3:+75885516 None:intergenic
AATGAAAGCTGGGGAGTTGT+TGG 0.360707 4.3:-75885395 MS.gene000764:CDS
TTTCATTGCTTTGATTGTAT+TGG 0.364692 4.3:+75886314 None:intergenic
GGAATGGTTTGTGCCTTTGC+TGG 0.368156 4.3:-75885136 MS.gene000764:CDS
TGAGAAATCCAAGCTTCATT+AGG 0.373854 4.3:-75885279 MS.gene000764:CDS
AGTGATTCGTTCCAAACATC+TGG 0.378012 4.3:-75885527 MS.gene000764:CDS
GCGTTTCCAAAATAATCTTC+TGG 0.379778 4.3:+75885439 None:intergenic
GCCAGCAAGAGGTTCGAAAA+AGG 0.381613 4.3:+75886149 None:intergenic
GAAACCAACGCTCTAGTGTT+GGG 0.385754 4.3:+75885779 None:intergenic
CTAGCTGACGGCATCTTCAT+AGG 0.398680 4.3:-75885904 MS.gene000764:CDS
GGGAGTTGTTGGAGGAAGGT+GGG 0.400037 4.3:-75885384 MS.gene000764:CDS
GAAACGCGATTATAGGTTGC+GGG 0.410719 4.3:-75885423 MS.gene000764:CDS
AAACCATGGAGAACTGCTAA+TGG 0.455624 4.3:+75885225 None:intergenic
GGGAAATGATCATGAATTCA+TGG 0.457595 4.3:-75885053 MS.gene000764:CDS
TTGTTGGAGGAAGGTGGGAT+TGG 0.457605 4.3:-75885379 MS.gene000764:CDS
GGGGAGTTGTTGGAGGAAGG+TGG 0.459892 4.3:-75885385 MS.gene000764:CDS
CGGAGTGGAAATAGAACAAA+TGG 0.464324 4.3:-75885157 MS.gene000764:CDS
TGCGGGTTTAAAATGAAAGC+TGG 0.465815 4.3:-75885406 MS.gene000764:CDS
GGCAATGGTGGAACAAGTCT+TGG 0.472119 4.3:+75885460 None:intergenic
CCAAAATAATCTTCTGGCAA+TGG 0.477773 4.3:+75885445 None:intergenic
GCTGGGGAGTTGTTGGAGGA+AGG 0.483907 4.3:-75885388 MS.gene000764:CDS
AAAACCCACATATGTTCCTT+CGG 0.485243 4.3:-75886010 MS.gene000764:CDS
GAGGAAGGTGGGATTGGTAA+AGG 0.486075 4.3:-75885373 MS.gene000764:CDS
CATTGCTTTGATTGTATTGG+TGG 0.486507 4.3:+75886317 None:intergenic
CTATCAAGCCTAATGAAGCT+TGG 0.491157 4.3:+75885271 None:intergenic
GGGGAAAGCCTGTGGCAGTT+CGG 0.491671 4.3:-75885177 MS.gene000764:CDS
TTGTATTGGTGGAGAGTACA+TGG 0.501073 4.3:+75886328 None:intergenic
ATGGGCTGTTGAATACCATC+TGG 0.501211 4.3:+75885757 None:intergenic
ATAAAATTGATTTAACTCCA+TGG 0.501405 4.3:-75886281 MS.gene000764:CDS
ATTGCCTCCAAAATCTTGTA+AGG 0.504306 4.3:+75885076 None:intergenic
TAAAATTGATTTAACTCCAT+GGG 0.505681 4.3:-75886280 MS.gene000764:CDS
GCCTTTGCTGGAATAGAAGA+AGG 0.511653 4.3:-75885124 MS.gene000764:CDS
TTCAATCATGTTGTAGCTGA+CGG 0.511810 4.3:-75885874 MS.gene000764:CDS
TATAGTTAAAGAAAATCGTA+TGG 0.514078 4.3:+75885738 None:intergenic
TTTCCACTCCGAACTGCCAC+AGG 0.522499 4.3:+75885169 None:intergenic
TGAATTTGGTCTGATGCTAC+AGG 0.526430 4.3:+75886204 None:intergenic
GGAAACCAACGCTCTAGTGT+TGG 0.530585 4.3:+75885778 None:intergenic
TGTAAGGAAGACACACTTGA+AGG 0.538009 4.3:+75885092 None:intergenic
TAAAGAATGATTATGAGTCA+TGG 0.538860 4.3:-75885303 MS.gene000764:CDS
GCGGGTTTAAAATGAAAGCT+GGG 0.538973 4.3:-75885405 MS.gene000764:CDS
GTATTAAGCGCATCTTCTTG+TGG 0.540410 4.3:+75885502 None:intergenic
AGGTACATCAAAATCATTGT+TGG 0.540953 4.3:-75885944 MS.gene000764:CDS
GAAAGCTGGGGAGTTGTTGG+AGG 0.541663 4.3:-75885392 MS.gene000764:CDS
TGGCCATTAGCAGTTCTCCA+TGG 0.541677 4.3:-75885228 MS.gene000764:CDS
AAATAATCTTCTGGCAATGG+TGG 0.542517 4.3:+75885448 None:intergenic
GGAAACGCGATTATAGGTTG+CGG 0.544739 4.3:-75885424 MS.gene000764:CDS
TAGTTTGTAACTCCGTTGAA+AGG 0.548129 4.3:+75885970 None:intergenic
CGTGTTCTGTAACTTTGAGA+CGG 0.548497 4.3:+75886127 None:intergenic
TGGAAATAGAACAAATGGAA+TGG 0.553380 4.3:-75885152 MS.gene000764:CDS
ACCTTCTTCTATTCCAGCAA+AGG 0.555908 4.3:+75885123 None:intergenic
AGTAGAAATTGGAGATCCCA+TGG 0.565431 4.3:+75886264 None:intergenic
ATGGCATTTCATCAATTCGT+GGG 0.570140 4.3:-75885842 MS.gene000764:CDS
AACAACCGAAGGAACATATG+TGG 0.575430 4.3:+75886005 None:intergenic
ATAGTTAAAGAAAATCGTAT+GGG 0.586579 4.3:+75885739 None:intergenic
AAACCAACGCTCTAGTGTTG+GGG 0.588825 4.3:+75885780 None:intergenic
CTACAGGATGGTGATAAAGA+AGG 0.592406 4.3:+75886220 None:intergenic
CATGGCATTTCATCAATTCG+TGG 0.610417 4.3:-75885843 MS.gene000764:CDS
ACTTTGAGACGGCCAGCAAG+AGG 0.616523 4.3:+75886138 None:intergenic
CTAGAGCGTTGGTTTCCAGA+TGG 0.617733 4.3:-75885772 MS.gene000764:CDS
TCTGTAACATGCAATAATGC+AGG 0.619827 4.3:-75886084 MS.gene000764:CDS
ATTGTTGGCAGTTCAAGTGA+CGG 0.619862 4.3:-75885929 MS.gene000764:CDS
AACCAACGCTCTAGTGTTGG+GGG 0.632370 4.3:+75885781 None:intergenic
TAGCTGACGGACAGTCCACA+TGG 0.634434 4.3:-75885861 MS.gene000764:CDS
CAAGTGACGGAGCTAGCTGA+CGG 0.639821 4.3:-75885916 MS.gene000764:CDS
CGGGTTTAAAATGAAAGCTG+GGG 0.642028 4.3:-75885404 MS.gene000764:CDS
AAGCCTGTGGCAGTTCGGAG+TGG 0.647498 4.3:-75885172 MS.gene000764:CDS
ACAACCGAAGGAACATATGT+GGG 0.650889 4.3:+75886006 None:intergenic
AACGGAGTTACAAACTACGA+AGG 0.656439 4.3:-75885964 MS.gene000764:CDS
TTACCATAAACATCAAACCA+TGG 0.659822 4.3:+75885211 None:intergenic
TAGCATCAGACCAAATTCAA+CGG 0.663707 4.3:-75886200 MS.gene000764:CDS
AGGTGTAAACTTTATACATG+CGG 0.677512 4.3:-75886064 MS.gene000764:CDS
GCCACAAAAGAATTACTCTG+AGG 0.711684 4.3:-75885713 MS.gene000764:CDS
CGAATTGATGAAATGCCATG+TGG 0.718082 4.3:+75885846 None:intergenic
TTTGGTCTGATGCTACAGGA+TGG 0.756053 4.3:+75886208 None:intergenic
TCGTGGGCTGAAATGTCACG+TGG 0.758657 4.3:-75885826 MS.gene000764:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTTAAAGAAAATCGTAT+GGG + chr4.3:75885642-75885661 None:intergenic 20.0%
!! TATAGTTAAAGAAAATCGTA+TGG + chr4.3:75885643-75885662 None:intergenic 20.0%
!!! ATAAAATTGATTTAACTCCA+TGG - chr4.3:75885097-75885116 MS.gene000764:CDS 20.0%
!!! TAAAATTGATTTAACTCCAT+GGG - chr4.3:75885098-75885117 MS.gene000764:CDS 20.0%
!!! TTGTAGAGAAGATATTTTTT+TGG + chr4.3:75885805-75885824 None:intergenic 20.0%
! AAAAATTCTATCTATCTGAG+AGG - chr4.3:75885715-75885734 MS.gene000764:CDS 25.0%
! TAAAGAATGATTATGAGTCA+TGG - chr4.3:75886075-75886094 MS.gene000764:CDS 25.0%
! TTTCATTGCTTTGATTGTAT+TGG + chr4.3:75885067-75885086 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG - chr4.3:75886176-75886195 MS.gene000764:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG - chr4.3:75886181-75886200 MS.gene000764:CDS 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG - chr4.3:75886180-75886199 MS.gene000764:CDS 25.0%
AGGTACATCAAAATCATTGT+TGG - chr4.3:75885434-75885453 MS.gene000764:CDS 30.0%
AGGTGTAAACTTTATACATG+CGG - chr4.3:75885314-75885333 MS.gene000764:CDS 30.0%
CTTTATCCAACCAACAAAAA+AGG - chr4.3:75885135-75885154 MS.gene000764:CDS 30.0%
TGATCATGAATTCATGGATA+TGG - chr4.3:75886331-75886350 MS.gene000764:CDS 30.0%
TGGAAATAGAACAAATGGAA+TGG - chr4.3:75886226-75886245 MS.gene000764:CDS 30.0%
TGGTTGGATAAAGTAGAAAT+TGG + chr4.3:75885128-75885147 None:intergenic 30.0%
TTACCATAAACATCAAACCA+TGG + chr4.3:75886170-75886189 None:intergenic 30.0%
!! TATGGTAATGATTTTGGATG+GGG - chr4.3:75886182-75886201 MS.gene000764:CDS 30.0%
!! TATTTTGGAAACGCGATTAT+AGG - chr4.3:75885948-75885967 MS.gene000764:CDS 30.0%
!!! ATAAAGAAGGCCTTTTTTGT+TGG + chr4.3:75885148-75885167 None:intergenic 30.0%
!!! CGTTCATTTTTTCCTTTCAA+CGG - chr4.3:75885396-75885415 MS.gene000764:CDS 30.0%
!!! CTTGAGCACTAAGAATTTTT+TGG - chr4.3:75886130-75886149 MS.gene000764:CDS 30.0%
AAAAATGAACGAACAACCGA+AGG + chr4.3:75885387-75885406 None:intergenic 35.0%
AAAACCCACATATGTTCCTT+CGG - chr4.3:75885368-75885387 MS.gene000764:CDS 35.0%
AAATAATCTTCTGGCAATGG+TGG + chr4.3:75885933-75885952 None:intergenic 35.0%
AAGAGGTTCGAAAAAGGAAA+GGG + chr4.3:75885226-75885245 None:intergenic 35.0%
AATGTCTTAGCCGTTGAATT+TGG + chr4.3:75885191-75885210 None:intergenic 35.0%
ATGGCATTTCATCAATTCGT+GGG - chr4.3:75885536-75885555 MS.gene000764:CDS 35.0%
ATTGCCTCCAAAATCTTGTA+AGG + chr4.3:75886305-75886324 None:intergenic 35.0%
CATTGCTTTGATTGTATTGG+TGG + chr4.3:75885064-75885083 None:intergenic 35.0%
CCAAAATAATCTTCTGGCAA+TGG + chr4.3:75885936-75885955 None:intergenic 35.0%
GCGCTTAATACGTTCATAAT+AGG - chr4.3:75885888-75885907 MS.gene000764:CDS 35.0%
GCGTTTCCAAAATAATCTTC+TGG + chr4.3:75885942-75885961 None:intergenic 35.0%
GGGAAATGATCATGAATTCA+TGG - chr4.3:75886325-75886344 MS.gene000764:CDS 35.0%
TAGCATCAGACCAAATTCAA+CGG - chr4.3:75885178-75885197 MS.gene000764:CDS 35.0%
TCTCCATGGTTTGATGTTTA+TGG - chr4.3:75886164-75886183 MS.gene000764:CDS 35.0%
TCTGTAACATGCAATAATGC+AGG - chr4.3:75885294-75885313 MS.gene000764:CDS 35.0%
TGAGAAATCCAAGCTTCATT+AGG - chr4.3:75886099-75886118 MS.gene000764:CDS 35.0%
TTCAATCATGTTGTAGCTGA+CGG - chr4.3:75885504-75885523 MS.gene000764:CDS 35.0%
! AAGGAACATATGTGGGTTTT+AGG + chr4.3:75885368-75885387 None:intergenic 35.0%
! CCATTGCCAGAAGATTATTT+TGG - chr4.3:75885933-75885952 MS.gene000764:CDS 35.0%
! GTGTCTTCCTTACAAGATTT+TGG - chr4.3:75886295-75886314 MS.gene000764:CDS 35.0%
! TAGTTTGTAACTCCGTTGAA+AGG + chr4.3:75885411-75885430 None:intergenic 35.0%
! TCCTCAGAGTAATTCTTTTG+TGG + chr4.3:75885669-75885688 None:intergenic 35.0%
! TCTTCCTTACAAGATTTTGG+AGG - chr4.3:75886298-75886317 MS.gene000764:CDS 35.0%
!! AAGCGCTTTTAACTCATCTT+TGG - chr4.3:75885823-75885842 MS.gene000764:CDS 35.0%
!! TACAAGATTTTGGAGGCAAT+GGG - chr4.3:75886305-75886324 MS.gene000764:CDS 35.0%
!! TTACAAGATTTTGGAGGCAA+TGG - chr4.3:75886304-75886323 MS.gene000764:CDS 35.0%
AAACCATGGAGAACTGCTAA+TGG + chr4.3:75886156-75886175 None:intergenic 40.0%
AACAACCGAAGGAACATATG+TGG + chr4.3:75885376-75885395 None:intergenic 40.0%
AACGGAGTTACAAACTACGA+AGG - chr4.3:75885414-75885433 MS.gene000764:CDS 40.0%
ACAACCGAAGGAACATATGT+GGG + chr4.3:75885375-75885394 None:intergenic 40.0%
ACCTTCTTCTATTCCAGCAA+AGG + chr4.3:75886258-75886277 None:intergenic 40.0%
AGTAGAAATTGGAGATCCCA+TGG + chr4.3:75885117-75885136 None:intergenic 40.0%
AGTGATTCGTTCCAAACATC+TGG - chr4.3:75885851-75885870 MS.gene000764:CDS 40.0%
ATTGTTGGCAGTTCAAGTGA+CGG - chr4.3:75885449-75885468 MS.gene000764:CDS 40.0%
CAAGAGGTTCGAAAAAGGAA+AGG + chr4.3:75885227-75885246 None:intergenic 40.0%
CATGGCATTTCATCAATTCG+TGG - chr4.3:75885535-75885554 MS.gene000764:CDS 40.0%
CGAATTGATGAAATGCCATG+TGG + chr4.3:75885535-75885554 None:intergenic 40.0%
CGGAGTGGAAATAGAACAAA+TGG - chr4.3:75886221-75886240 MS.gene000764:CDS 40.0%
CTACAGGATGGTGATAAAGA+AGG + chr4.3:75885161-75885180 None:intergenic 40.0%
CTATCAAGCCTAATGAAGCT+TGG + chr4.3:75886110-75886129 None:intergenic 40.0%
GCCACAAAAGAATTACTCTG+AGG - chr4.3:75885665-75885684 MS.gene000764:CDS 40.0%
GTATTAAGCGCATCTTCTTG+TGG + chr4.3:75885879-75885898 None:intergenic 40.0%
TGAATTTGGTCTGATGCTAC+AGG + chr4.3:75885177-75885196 None:intergenic 40.0%
TGTAAGGAAGACACACTTGA+AGG + chr4.3:75886289-75886308 None:intergenic 40.0%
TTCTTGTGGATCCAGATGTT+TGG + chr4.3:75885865-75885884 None:intergenic 40.0%
! CGGGTTTAAAATGAAAGCTG+GGG - chr4.3:75885974-75885993 MS.gene000764:CDS 40.0%
! CGTGTTCTGTAACTTTGAGA+CGG + chr4.3:75885254-75885273 None:intergenic 40.0%
! GCGGGTTTAAAATGAAAGCT+GGG - chr4.3:75885973-75885992 MS.gene000764:CDS 40.0%
! TGCGGGTTTAAAATGAAAGC+TGG - chr4.3:75885972-75885991 MS.gene000764:CDS 40.0%
! TTGTATTGGTGGAGAGTACA+TGG + chr4.3:75885053-75885072 None:intergenic 40.0%
!!! AGAAGGCCTTTTTTGTTGGT+TGG + chr4.3:75885144-75885163 None:intergenic 40.0%
AAACCAACGCTCTAGTGTTG+GGG + chr4.3:75885601-75885620 None:intergenic 45.0%
AATGAAAGCTGGGGAGTTGT+TGG - chr4.3:75885983-75886002 MS.gene000764:CDS 45.0%
GAAACCAACGCTCTAGTGTT+GGG + chr4.3:75885602-75885621 None:intergenic 45.0%
GAAACGCGATTATAGGTTGC+GGG - chr4.3:75885955-75885974 MS.gene000764:CDS 45.0%
GCCTTTGCTGGAATAGAAGA+AGG - chr4.3:75886254-75886273 MS.gene000764:CDS 45.0%
GGAAACGCGATTATAGGTTG+CGG - chr4.3:75885954-75885973 MS.gene000764:CDS 45.0%
TTTGGTCTGATGCTACAGGA+TGG + chr4.3:75885173-75885192 None:intergenic 45.0%
! ATGGGCTGTTGAATACCATC+TGG + chr4.3:75885624-75885643 None:intergenic 45.0%
!!! TCCTTTTTCGAACCTCTTGC+TGG - chr4.3:75885228-75885247 MS.gene000764:CDS 45.0%
CTAGCTGACGGCATCTTCAT+AGG - chr4.3:75885474-75885493 MS.gene000764:CDS 50.0%
GCCAGCAAGAGGTTCGAAAA+AGG + chr4.3:75885232-75885251 None:intergenic 50.0%
GGAAACCAACGCTCTAGTGT+TGG + chr4.3:75885603-75885622 None:intergenic 50.0%
TGGCCATTAGCAGTTCTCCA+TGG - chr4.3:75886150-75886169 MS.gene000764:CDS 50.0%
TTGTTGGAGGAAGGTGGGAT+TGG - chr4.3:75885999-75886018 MS.gene000764:CDS 50.0%
! AACCAACGCTCTAGTGTTGG+GGG + chr4.3:75885600-75885619 None:intergenic 50.0%
! GAGGAAGGTGGGATTGGTAA+AGG - chr4.3:75886005-75886024 MS.gene000764:CDS 50.0%
! GGAATGGTTTGTGCCTTTGC+TGG - chr4.3:75886242-75886261 MS.gene000764:CDS 50.0%
! GGCAATGGTGGAACAAGTCT+TGG + chr4.3:75885921-75885940 None:intergenic 50.0%
! TTTTGGATGGGGAAAGCCTG+TGG - chr4.3:75886193-75886212 MS.gene000764:CDS 50.0%
!! CTAGAGCGTTGGTTTCCAGA+TGG - chr4.3:75885606-75885625 MS.gene000764:CDS 50.0%
AACCCCCAACACTAGAGCGT+TGG - chr4.3:75885595-75885614 MS.gene000764:CDS 55.0%
GAAAGCTGGGGAGTTGTTGG+AGG - chr4.3:75885986-75886005 MS.gene000764:CDS 55.0%
GGGAGTTGTTGGAGGAAGGT+GGG - chr4.3:75885994-75886013 MS.gene000764:CDS 55.0%
TTTCCACTCCGAACTGCCAC+AGG + chr4.3:75886212-75886231 None:intergenic 55.0%
! CAAGTGACGGAGCTAGCTGA+CGG - chr4.3:75885462-75885481 MS.gene000764:CDS 55.0%
! TAGCTGACGGACAGTCCACA+TGG - chr4.3:75885517-75885536 MS.gene000764:CDS 55.0%
! TCGTGGGCTGAAATGTCACG+TGG - chr4.3:75885552-75885571 MS.gene000764:CDS 55.0%
!! ACTTTGAGACGGCCAGCAAG+AGG + chr4.3:75885243-75885262 None:intergenic 55.0%
AAGCCTGTGGCAGTTCGGAG+TGG - chr4.3:75886206-75886225 MS.gene000764:CDS 60.0%
GCTGGGGAGTTGTTGGAGGA+AGG - chr4.3:75885990-75886009 MS.gene000764:CDS 60.0%
GGGGAAAGCCTGTGGCAGTT+CGG - chr4.3:75886201-75886220 MS.gene000764:CDS 60.0%
GGGGAGTTGTTGGAGGAAGG+TGG - chr4.3:75885993-75886012 MS.gene000764:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 75885039 75886361 75885039 ID=MS.gene000764
chr4.3 mRNA 75885039 75886361 75885039 ID=MS.gene000764.t1;Parent=MS.gene000764
chr4.3 exon 75885039 75886361 75885039 ID=MS.gene000764.t1.exon1;Parent=MS.gene000764.t1
chr4.3 CDS 75885039 75886361 75885039 ID=cds.MS.gene000764.t1;Parent=MS.gene000764.t1
Gene Sequence

>MS.gene000764

ATGGGTACATTCCATGTACTCTCCACCAATACAATCAAAGCAATGAAATCAAGTAATCATAAAATTGATTTAACTCCATGGGATCTCCAATTTCTACTTTATCCAACCAACAAAAAAGGCCTTCTTTATCACCATCCTGTAGCATCAGACCAAATTCAACGGCTAAGACATTCTCTTTCTTCTGCCCTTTCCTTTTTCGAACCTCTTGCTGGCCGTCTCAAAGTTACAGAACACGAAGACAGTACTGTCTCGTGTTCTGTAACATGCAATAATGCAGGTGTAAACTTTATACATGCGGCAGCAAAGAATACTTGTGTTGCTGACATCCTAAAACCCACATATGTTCCTTCGGTTGTTCGTTCATTTTTTCCTTTCAACGGAGTTACAAACTACGAAGGTACATCAAAATCATTGTTGGCAGTTCAAGTGACGGAGCTAGCTGACGGCATCTTCATAGGCTGTAATTTCAATCATGTTGTAGCTGACGGACAGTCCACATGGCATTTCATCAATTCGTGGGCTGAAATGTCACGTGGTTGTCATCATCAAATATCTAAACCCCCAACACTAGAGCGTTGGTTTCCAGATGGTATTCAACAGCCCATACGATTTTCTTTAACTATAGAGCCACAAAAGAATTACTCTGAGGAGTTGAGTTTTTCGTCATCTGATGACGAAAAATTCTATCTATCTGAGAGGATATTTCACTTCACAAAGAAGAAAATCATGCAATTGAAATCAAAAGTCAACGCAGCGATTGATACCAAAAAAATATCTTCTCTACAAGCGCTTTTAACTCATCTTTGGTGTTCAGTGATTCGTTCCAAACATCTGGATCCACAAGAAGATGCGCTTAATACGTTCATAATAGGAGTTAGACCAAGACTTGTTCCACCATTGCCAGAAGATTATTTTGGAAACGCGATTATAGGTTGCGGGTTTAAAATGAAAGCTGGGGAGTTGTTGGAGGAAGGTGGGATTGGTAAAGGTGCTTTGTTGATGAATAAGTTAATTGCTTTGCACTCTAATGAGAAATTAAAGAATGATTATGAGTCATGGTTGAGAAATCCAAGCTTCATTAGGCTTGATAGCTTGAGCACTAAGAATTTTTTGGCCATTAGCAGTTCTCCATGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAGCCTGTGGCAGTTCGGAGTGGAAATAGAACAAATGGAATGGTTTGTGCCTTTGCTGGAATAGAAGAAGGTAGCATTGACCTTCAAGTGTGTCTTCCTTACAAGATTTTGGAGGCAATGGGAAATGATCATGAATTCATGGATATGGTTAACTAA
Protein sequence

>MS.gene000764.t1

MGTFHVLSTNTIKAMKSSNHKIDLTPWDLQFLLYPTNKKGLLYHHPVASDQIQRLRHSLSSALSFFEPLAGRLKVTEHEDSTVSCSVTCNNAGVNFIHAAAKNTCVADILKPTYVPSVVRSFFPFNGVTNYEGTSKSLLAVQVTELADGIFIGCNFNHVVADGQSTWHFINSWAEMSRGCHHQISKPPTLERWFPDGIQQPIRFSLTIEPQKNYSEELSFSSSDDEKFYLSERIFHFTKKKIMQLKSKVNAAIDTKKISSLQALLTHLWCSVIRSKHLDPQEDALNTFIIGVRPRLVPPLPEDYFGNAIIGCGFKMKAGELLEEGGIGKGALLMNKLIALHSNEKLKNDYESWLRNPSFIRLDSLSTKNFLAISSSPWFDVYGNDFGWGKPVAVRSGNRTNGMVCAFAGIEEGSIDLQVCLPYKILEAMGNDHEFMDMVN