AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066657

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066657.t1 MTR_4g022800 87.810 443 53 1 1 442 1 443 0.0 812
MS.gene066657.t1 MTR_4g022720 87.783 442 48 1 1 442 1 436 0.0 806
MS.gene066657.t1 MTR_7g026860 86.425 442 57 1 1 442 1 439 0.0 799
MS.gene066657.t1 MTR_4g022670 83.937 442 71 0 1 442 1 442 0.0 781
MS.gene066657.t1 MTR_4g022825 75.737 441 106 1 1 441 1 440 0.0 698
MS.gene066657.t1 MTR_4g022760 76.471 442 60 1 1 442 1 398 0.0 684
MS.gene066657.t1 MTR_3g088420 68.778 442 130 2 1 442 1 434 0.0 623
MS.gene066657.t1 MTR_3g026310 67.038 449 141 2 1 442 1 449 0.0 612
MS.gene066657.t1 MTR_8g041770 59.382 453 162 6 1 442 1 442 0.0 531
MS.gene066657.t1 MTR_0190s0020 58.186 452 168 7 1 441 1 442 0.0 530
MS.gene066657.t1 MTR_4g094842 56.893 457 168 10 1 439 1 446 5.50e-172 489
MS.gene066657.t1 MTR_4g094835 52.527 455 185 8 1 437 1 442 4.49e-163 467
MS.gene066657.t1 MTR_6g016815 52.941 442 134 7 1 442 15 382 1.58e-149 430
MS.gene066657.t1 MTR_4g094838 49.672 457 178 9 4 437 1 428 4.86e-147 426
MS.gene066657.t1 MTR_8g079710 50.993 453 184 11 1 432 1 436 6.95e-145 421
MS.gene066657.t1 MTR_4g094828 50.108 463 196 14 1 441 1 450 4.40e-144 419
MS.gene066657.t1 MTR_4g022290 49.675 461 192 13 1 439 1 443 2.70e-143 417
MS.gene066657.t1 MTR_4g094832 55.526 380 142 6 72 437 60 426 8.98e-140 407
MS.gene066657.t1 MTR_3g101900 40.961 437 233 12 18 441 24 448 2.40e-97 299
MS.gene066657.t1 MTR_3g101930 40.674 445 232 11 16 440 22 454 3.04e-95 294
MS.gene066657.t1 MTR_7g026850 79.641 167 34 0 29 195 12 178 6.69e-94 281
MS.gene066657.t1 MTR_3g102020 39.593 442 240 10 16 441 21 451 2.17e-91 284
MS.gene066657.t1 MTR_7g026770 69.697 198 32 1 141 338 132 301 3.15e-90 276
MS.gene066657.t1 MTR_3g101960 39.588 437 236 10 16 436 21 445 8.55e-89 278
MS.gene066657.t1 MTR_8g040760 63.850 213 70 3 1 207 1 212 9.36e-89 269
MS.gene066657.t1 MTR_3g101970 38.409 440 232 10 16 440 21 436 7.80e-87 272
MS.gene066657.t1 MTR_3g102010 38.914 442 242 12 16 441 22 451 9.48e-87 272
MS.gene066657.t1 MTR_8g040780 59.447 217 84 2 224 436 11 227 2.49e-82 254
MS.gene066657.t1 MTR_4g015180 33.947 489 242 15 16 442 19 488 3.33e-63 212
MS.gene066657.t1 MTR_5g081960 30.470 489 256 13 16 441 19 486 3.76e-59 201
MS.gene066657.t1 MTR_3g070170 30.323 465 266 10 16 441 25 470 1.80e-57 196
MS.gene066657.t1 MTR_4g094825 51.323 189 83 5 17 199 84 269 1.16e-53 184
MS.gene066657.t1 MTR_4g094825 49.367 158 67 2 286 441 245 391 1.40e-44 160
MS.gene066657.t1 MTR_3g102030 34.242 330 175 7 73 397 28 320 1.89e-48 168
MS.gene066657.t1 MTR_6g034495 56.774 155 58 3 33 179 8 161 1.15e-47 161
MS.gene066657.t1 MTR_7g026800 52.096 167 50 2 212 377 19 156 1.48e-46 159
MS.gene066657.t1 MTR_6g034485 62.121 132 42 2 189 320 2 125 2.54e-46 156
MS.gene066657.t1 MTR_6g045553 28.330 473 285 13 4 437 14 471 6.76e-46 166
MS.gene066657.t1 MTR_4g022780 50.276 181 57 4 200 377 7 157 7.05e-46 157
MS.gene066657.t1 MTR_7g026830 82.353 85 15 0 82 166 14 98 9.55e-43 146
MS.gene066657.t1 MTR_7g026780 65.766 111 14 2 332 442 26 112 5.90e-39 136
MS.gene066657.t1 MTR_4g007540 26.633 398 247 14 55 432 56 428 3.58e-30 121
MS.gene066657.t1 MTR_8g075610 26.031 388 256 11 55 431 47 414 3.21e-29 119
MS.gene066657.t1 MTR_2g064495 26.110 383 265 10 55 431 56 426 1.60e-28 117
MS.gene066657.t1 MTR_2g105330 26.110 383 265 10 55 431 56 426 1.85e-28 116
MS.gene066657.t1 MTR_7g026870 90.741 54 5 0 298 351 98 151 7.71e-28 108
MS.gene066657.t1 MTR_8g096530 28.198 344 225 8 53 392 57 382 2.08e-26 111
MS.gene066657.t1 MTR_4g013050 28.297 364 187 13 53 393 51 363 1.22e-23 102
MS.gene066657.t1 MTR_3g026950 27.273 385 206 18 52 393 51 404 1.23e-23 103
MS.gene066657.t1 MTR_3g098980 26.316 399 237 16 55 431 66 429 2.18e-22 99.4
MS.gene066657.t1 MTR_7g026880 71.154 52 15 0 390 441 2 53 1.96e-20 84.7
MS.gene066657.t1 MTR_3g049150 26.963 382 244 14 41 399 37 406 3.76e-20 92.8
MS.gene066657.t1 MTR_1g081660 24.457 368 236 15 37 393 60 396 8.08e-19 89.0
MS.gene066657.t1 MTR_2g089765 23.666 431 277 14 3 392 15 434 2.15e-18 87.8
MS.gene066657.t1 MTR_7g027175 23.018 391 273 11 50 432 63 433 5.40e-18 86.3
MS.gene066657.t1 MTR_7g084940 25.762 361 235 13 45 392 52 392 5.84e-18 86.3
MS.gene066657.t1 MTR_3g465980 27.143 280 178 10 55 324 68 331 6.12e-18 86.3
MS.gene066657.t1 MTR_2g089755 24.757 412 256 17 24 396 37 433 2.65e-17 84.3
MS.gene066657.t1 MTR_2g089670 26.733 404 246 17 22 392 43 429 3.32e-17 84.0
MS.gene066657.t1 MTR_6g015830 25.272 459 278 16 10 430 21 452 3.73e-17 84.0
MS.gene066657.t1 MTR_7g014230 26.995 426 232 17 18 396 22 415 4.86e-17 83.6
MS.gene066657.t1 MTR_2g089650 23.251 443 288 15 8 418 21 443 9.76e-17 82.4
MS.gene066657.t1 MTR_4g097090 24.934 377 222 15 55 398 65 413 1.01e-16 82.4
MS.gene066657.t1 MTR_7g014330 25.118 422 245 16 18 396 20 413 1.66e-16 81.6
MS.gene066657.t1 MTR_7g114470 22.274 431 281 16 25 425 25 431 1.86e-16 81.6
MS.gene066657.t1 MTR_7g015860 24.027 437 257 18 4 393 5 413 3.39e-16 80.9
MS.gene066657.t1 MTR_5g076700 24.013 304 198 9 25 310 24 312 4.17e-16 80.5
MS.gene066657.t1 MTR_7g014250 24.879 414 254 16 19 396 21 413 4.29e-16 80.5
MS.gene066657.t1 MTR_7g114550 24.711 433 272 17 25 428 24 431 4.93e-16 80.5
MS.gene066657.t1 MTR_3g086020 26.856 458 268 23 4 441 5 415 1.38e-15 78.6
MS.gene066657.t1 MTR_4g055170 26.861 309 192 9 55 351 69 355 2.44e-15 78.2
MS.gene066657.t1 MTR_1g040175 25.806 279 189 8 50 324 59 323 9.00e-15 76.6
MS.gene066657.t1 MTR_7g114520 23.077 429 277 17 25 432 25 421 9.97e-15 76.3
MS.gene066657.t1 MTR_3g071320 26.254 339 205 13 22 334 24 343 1.12e-14 76.3
MS.gene066657.t1 MTR_6g015320 24.242 363 240 11 52 395 63 409 2.41e-14 75.1
MS.gene066657.t1 MTR_7g086000 26.685 371 223 17 58 398 63 414 6.73e-14 73.9
MS.gene066657.t1 MTR_3g049140 27.222 180 113 6 235 399 27 203 7.19e-14 71.6
MS.gene066657.t1 MTR_3g087640 24.738 477 291 19 1 428 5 462 9.59e-14 73.6
MS.gene066657.t1 MTR_1g053315 23.116 398 250 18 55 424 78 447 1.21e-13 73.2
MS.gene066657.t1 MTR_7g014160 25.000 460 270 16 15 422 19 455 1.58e-13 72.8
MS.gene066657.t1 MTR_7g084910 26.316 285 170 10 45 308 53 318 1.99e-13 72.4
MS.gene066657.t1 MTR_1g040320 25.616 406 223 19 44 430 51 396 4.67e-13 70.9
MS.gene066657.t1 MTR_6g015815 23.620 453 284 15 15 430 25 452 6.24e-13 70.9
MS.gene066657.t1 MTR_7g085930 21.411 397 251 10 43 399 47 422 2.37e-12 68.9
MS.gene066657.t1 MTR_6g053810 24.561 285 181 11 55 324 71 336 2.80e-12 68.9
MS.gene066657.t1 MTR_5g090810 22.892 415 254 15 55 424 66 459 8.48e-12 67.4
MS.gene066657.t1 MTR_7g085990 21.446 401 246 11 43 399 43 418 1.20e-11 66.6
MS.gene066657.t1 MTR_7g014200 22.894 463 285 15 15 428 19 458 1.36e-11 66.6
MS.gene066657.t1 MTR_7g013850 24.194 434 254 17 50 431 63 473 1.37e-11 66.6
MS.gene066657.t1 MTR_8g467580 25.000 460 289 20 4 437 3 432 3.09e-11 65.5
MS.gene066657.t1 MTR_2g015430 22.251 391 255 13 19 395 31 386 4.38e-11 65.1
MS.gene066657.t1 MTR_5g090830 23.040 421 269 13 55 441 66 465 8.43e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066657.t1 AT5G67150 39.474 456 249 12 1 440 1 445 5.16e-100 306
MS.gene066657.t1 AT3G50300 41.591 440 223 15 1 422 1 424 1.04e-95 295
MS.gene066657.t1 AT3G50280 37.639 449 258 10 1 437 1 439 1.19e-90 282
MS.gene066657.t1 AT5G67160 37.613 444 246 10 6 441 12 432 2.08e-88 276
MS.gene066657.t1 AT5G42830 37.045 440 253 10 15 441 21 449 1.41e-86 271
MS.gene066657.t1 AT3G50270 37.389 452 257 11 6 441 7 448 2.05e-86 271
MS.gene066657.t1 AT5G07850 39.775 445 239 13 15 442 22 454 1.32e-85 269
MS.gene066657.t1 AT5G07860 37.691 459 246 12 4 440 8 448 1.35e-84 266
MS.gene066657.t1 AT5G38130 38.073 436 242 12 1 422 21 442 1.08e-83 265
MS.gene066657.t1 AT5G07870 37.029 478 239 17 4 442 8 462 7.05e-77 247
MS.gene066657.t1 AT2G39980 33.264 481 247 13 16 442 21 481 7.32e-70 229
MS.gene066657.t1 AT5G01210 34.856 416 212 11 66 441 73 469 6.01e-69 227
MS.gene066657.t1 AT3G50280 37.931 319 182 6 125 437 1 309 1.11e-63 208
MS.gene066657.t1 AT3G50290 41.606 274 147 6 122 390 4 269 5.77e-55 184
MS.gene066657.t1 AT5G23940 29.801 453 292 13 4 437 3 448 3.51e-50 177
MS.gene066657.t1 AT5G48930 26.750 400 273 10 41 431 39 427 8.21e-32 126
MS.gene066657.t1 AT3G48720 26.733 404 260 14 42 431 44 425 6.21e-24 103
MS.gene066657.t1 AT5G57840 26.437 435 276 18 11 422 9 422 3.16e-22 99.0
MS.gene066657.t1 AT5G41040 24.190 401 266 13 52 439 66 441 1.53e-21 97.1
MS.gene066657.t1 AT5G41040 24.814 403 261 15 52 439 82 457 1.72e-21 97.1
MS.gene066657.t1 AT3G03480 26.764 411 246 18 25 411 33 412 3.39e-20 93.2
MS.gene066657.t1 AT3G29670 23.146 445 298 14 3 422 8 433 4.40e-20 92.8
MS.gene066657.t1 AT3G29590 25.000 460 283 15 3 428 7 438 1.34e-18 88.2
MS.gene066657.t1 AT5G63560 24.745 392 251 15 55 432 61 422 1.77e-18 87.8
MS.gene066657.t1 AT2G40230 24.719 445 303 16 1 432 1 426 1.23e-17 85.1
MS.gene066657.t1 AT1G65450 26.129 310 201 13 53 349 70 364 2.14e-17 84.7
MS.gene066657.t1 AT3G26040 25.000 416 246 16 51 441 55 429 3.11e-17 84.0
MS.gene066657.t1 AT5G23970 26.154 455 275 22 4 437 3 417 1.02e-16 82.4
MS.gene066657.t1 AT5G39090 26.087 276 170 10 135 392 140 399 4.48e-16 80.5
MS.gene066657.t1 AT1G28680 24.566 403 251 16 15 395 18 389 6.93e-16 80.1
MS.gene066657.t1 AT3G29635 24.598 435 273 15 21 422 26 438 8.45e-16 79.7
MS.gene066657.t1 AT3G29680 24.619 394 253 12 52 422 59 431 3.81e-15 77.8
MS.gene066657.t1 AT5G61160 23.881 402 249 15 20 393 31 403 4.04e-15 77.4
MS.gene066657.t1 AT1G28680 25.602 332 200 13 15 325 18 323 6.40e-15 76.6
MS.gene066657.t1 AT3G30280 25.061 411 250 20 54 441 61 436 1.20e-14 76.3
MS.gene066657.t1 AT1G27620 24.422 389 258 16 55 431 58 422 1.98e-14 75.5
MS.gene066657.t1 AT5G16410 24.694 409 232 19 41 414 77 444 2.43e-14 75.5
MS.gene066657.t1 AT2G25150 24.722 360 240 12 50 396 62 403 1.30e-13 72.8
MS.gene066657.t1 AT5G07080 25.231 325 203 13 17 322 28 331 1.39e-13 72.8
MS.gene066657.t1 AT1G03390 25.121 414 250 22 42 423 59 444 1.72e-13 72.4
MS.gene066657.t1 AT4G15390 22.596 416 249 17 53 437 62 435 3.81e-12 68.2
MS.gene066657.t1 AT4G15390 22.596 416 249 17 53 437 62 435 3.81e-12 68.2
MS.gene066657.t1 AT5G39050 23.988 321 191 11 149 428 160 468 4.15e-12 68.2

Find 88 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCATGGTTTGATGTTTA+TGG 0.113342 4.2:+80062490 MS.gene066657:CDS
CCATTACCAGAAAATTATTT+TGG 0.134239 4.2:+80062259 MS.gene066657:CDS
ACTTGCTTCAACCAATAAAA+AGG 0.152814 4.2:+80061457 MS.gene066657:CDS
TTAGCAAGCCTAACAAAATT+TGG 0.155332 4.2:-80062433 None:intergenic
ATAGTTTGATCACTTGATTT+TGG 0.265670 4.2:-80061404 None:intergenic
TTGTCTTCCACATGAGATTC+TGG 0.280245 4.2:+80062621 MS.gene066657:CDS
AAAACTTTGTTTATCAAATA+GGG 0.285623 4.2:+80062042 MS.gene066657:CDS
AACACTAGAGCGTTGGTTTC+CGG 0.290488 4.2:+80061928 MS.gene066657:CDS
GATGTTTATGGTAATGATTT+TGG 0.308658 4.2:+80062502 MS.gene066657:CDS
TTCTTGTGGATCAAATTGTT+TGG 0.335214 4.2:-80062188 None:intergenic
AGTGTCTCAGCCGTTGGATT+TGG 0.357834 4.2:-80061511 None:intergenic
AATGAAAGTTGGGGAGTTGT+TGG 0.379006 4.2:+80062309 MS.gene066657:CDS
TGGATTTGGTTAGCTTCAAC+TGG 0.379348 4.2:-80061497 None:intergenic
AAATGCAATTATAAGTTGTA+TGG 0.380673 4.2:+80062282 MS.gene066657:CDS
GTGTTTGCCGGAATAGAAAA+AGG 0.382441 4.2:+80062580 MS.gene066657:CDS
ACCTCTCACGTTGCATTAGT+TGG 0.383610 4.2:-80062683 None:intergenic
GCCAACAAGAGGTTGGAAAA+AGG 0.384487 4.2:-80061552 None:intergenic
AAACCATGGAGAACTGCTAA+TGG 0.390568 4.2:-80062479 None:intergenic
GTATGGTTAAAATGAAAGTT+GGG 0.393088 4.2:+80062299 MS.gene066657:CDS
GAAACCAACGCTCTAGTGTT+GGG 0.395003 4.2:-80061925 None:intergenic
TGTATGGTTAAAATGAAAGT+TGG 0.395906 4.2:+80062298 MS.gene066657:CDS
TATGGCATTTCATCAATTCA+TGG 0.407954 4.2:+80061858 MS.gene066657:CDS
CTAGTTGACGGTATGTTCAT+TGG 0.417115 4.2:+80061797 MS.gene066657:CDS
GGACTTGTTACAGTGTTTGC+CGG 0.422586 4.2:+80062568 MS.gene066657:CDS
GAGGTACATAGGTGGGTTCT+AGG 0.427095 4.2:-80061688 None:intergenic
TTTAACCATGTTGTCGTTGA+CGG 0.436978 4.2:+80061827 MS.gene066657:CDS
GACACAACATTCATGAATTG+AGG 0.451357 4.2:-80062661 None:intergenic
GTTGGGGAGTTGTTGGAGGA+AGG 0.453345 4.2:+80062316 MS.gene066657:CDS
AATACGATTTCCTTTCACTT+TGG 0.455150 4.2:+80061967 MS.gene066657:CDS
TTTAACTCATCTTTGGTGCT+CGG 0.456890 4.2:+80062156 MS.gene066657:CDS
CGTAGTGGATATAAAGCAAA+TGG 0.458289 4.2:+80062547 MS.gene066657:CDS
CTATTAAATACTTCTTCTTG+TGG 0.458740 4.2:-80062202 None:intergenic
GTATTTAATAGAGTCGTGAT+AGG 0.460162 4.2:+80062214 MS.gene066657:CDS
ACCTGCATTGTTGCATATAA+CGG 0.463614 4.2:-80061618 None:intergenic
TGGTTGAAGCAAGTAGAAAT+CGG 0.475965 4.2:-80061448 None:intergenic
TGAAGAATCACTACGAGTCT+TGG 0.477110 4.2:+80062401 MS.gene066657:CDS
GGGGAGTTGTTGGAGGAAGG+TGG 0.485977 4.2:+80062319 MS.gene066657:CDS
TATCCACTACGAACTGCTAC+AGG 0.486958 4.2:-80062535 None:intergenic
AATAGAGTCGTGATAGGAGT+TGG 0.491643 4.2:+80062220 MS.gene066657:CDS
CATGTGGAAGACAAACTTGA+AGG 0.493205 4.2:-80062612 None:intergenic
AGGCACATCAAAACCATTGT+TGG 0.495948 4.2:+80061757 MS.gene066657:CDS
AAACTATTGATTTGACTCCA+TGG 0.507614 4.2:+80061420 MS.gene066657:CDS
GAAAGTTGGGGAGTTGTTGG+AGG 0.511101 4.2:+80062312 MS.gene066657:CDS
AACTATTGATTTGACTCCAT+GGG 0.516810 4.2:+80061421 MS.gene066657:CDS
CAACAAAAGAATCACTCTGA+TGG 0.518700 4.2:+80061992 MS.gene066657:CDS
CACATCAAAACCATTGTTGG+CGG 0.519877 4.2:+80061760 MS.gene066657:CDS
ATGAATTGATGAAATGCCAT+AGG 0.530467 4.2:-80061856 None:intergenic
ATTGGCTGTTGAACACCATC+CGG 0.531798 4.2:-80061947 None:intergenic
ACATGAGATTCTGGAGGCAA+TGG 0.532230 4.2:+80062630 MS.gene066657:CDS
ATGGCATTTCATCAATTCAT+GGG 0.535218 4.2:+80061859 MS.gene066657:CDS
AAAGTGAAAGGAAATCGTAT+TGG 0.538100 4.2:-80061965 None:intergenic
ACAGAGAGTGTCTCAGCCGT+TGG 0.539317 4.2:-80061517 None:intergenic
GGAAACCAACGCTCTAGTGT+TGG 0.539690 4.2:-80061926 None:intergenic
GAGGAAGGTGGACTTTGTAA+AGG 0.546587 4.2:+80062331 MS.gene066657:CDS
TCCGTTATATGCAACAATGC+AGG 0.551070 4.2:+80061617 MS.gene066657:CDS
TGTTGGCCGTCTCAAAATAA+CGG 0.553833 4.2:+80061568 MS.gene066657:CDS
ATTGTTGGCGGTTCAAGTAA+CGG 0.553835 4.2:+80061772 MS.gene066657:CDS
AACAAAAGAATCACTCTGAT+GGG 0.558688 4.2:+80061993 MS.gene066657:CDS
TGAGACGGCCAACAAGAGGT+TGG 0.560059 4.2:-80061559 None:intergenic
GGTAATGGTGGAACTAACCT+TGG 0.563399 4.2:-80062244 None:intergenic
ACACAACATTCATGAATTGA+GGG 0.567594 4.2:-80062660 None:intergenic
AAACCAACGCTCTAGTGTTG+GGG 0.573903 4.2:-80061924 None:intergenic
GTGATTCTTCAACTTCTCAT+CGG 0.575796 4.2:-80062389 None:intergenic
TTTGGTTAGCTTCAACTGGA+TGG 0.576856 4.2:-80061493 None:intergenic
AGTAGAAATCGGAGATCCCA+TGG 0.579335 4.2:-80061437 None:intergenic
TCTCTCCACTGATACAATCA+AGG 0.580251 4.2:+80061379 MS.gene066657:CDS
TCGTTGACGGAAAGTCCCTA+TGG 0.585370 4.2:+80061840 MS.gene066657:CDS
TGGTGCCTTGATTGTATCAG+TGG 0.587630 4.2:-80061384 None:intergenic
GTTACTTGAACCGCCAACAA+TGG 0.593750 4.2:-80061770 None:intergenic
TATCCATTAGCAGTTCTCCA+TGG 0.597915 4.2:+80062476 MS.gene066657:CDS
TATGGTTAAAATGAAAGTTG+GGG 0.603920 4.2:+80062300 MS.gene066657:CDS
TGAATTGATGAAATGCCATA+GGG 0.604499 4.2:-80061855 None:intergenic
AAGTATTCTCAGCTGCAGCA+TGG 0.612633 4.2:-80061652 None:intergenic
ACAATTAGAGGTACATAGGT+GGG 0.616014 4.2:-80061695 None:intergenic
CAAGTAACGGAACTAGTTGA+CGG 0.616630 4.2:+80061785 MS.gene066657:CDS
ATCAGTGGAGAGAACTCGTA+CGG 0.619099 4.2:-80061369 None:intergenic
GACAATTAGAGGTACATAGG+TGG 0.624565 4.2:-80061696 None:intergenic
AACAAAGTTAGAAGCTACGA+AGG 0.627886 4.2:+80061737 MS.gene066657:CDS
ACTTTCCGTCAACGACAACA+TGG 0.629179 4.2:-80061832 None:intergenic
CTAGAGCGTTGGTTTCCGGA+TGG 0.634274 4.2:+80061932 MS.gene066657:CDS
AACCAACGCTCTAGTGTTGG+GGG 0.634809 4.2:-80061923 None:intergenic
ATCGACAATTAGAGGTACAT+AGG 0.637686 4.2:-80061699 None:intergenic
TCTTCCACATGAGATTCTGG+AGG 0.645425 4.2:+80062624 MS.gene066657:CDS
TTACCATAAACATCAAACCA+TGG 0.659822 4.2:-80062493 None:intergenic
AAGCCTGTAGCAGTTCGTAG+TGG 0.668630 4.2:+80062532 MS.gene066657:CDS
TCGTGATAGGAGTTGGACCA+AGG 0.676348 4.2:+80062227 MS.gene066657:CDS
TTGAAGCTAACCAAATCCAA+CGG 0.678953 4.2:+80061501 MS.gene066657:CDS
ATTGCCTCCAGAATCTCATG+TGG 0.679505 4.2:-80062628 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAACTTTGTTTATCAAAT+AGG + chr4.2:80062041-80062060 MS.gene066657:CDS 15.0%
!!! AAAACTTTGTTTATCAAATA+GGG + chr4.2:80062042-80062061 MS.gene066657:CDS 15.0%
!! AAATGCAATTATAAGTTGTA+TGG + chr4.2:80062282-80062301 MS.gene066657:CDS 20.0%
!! TTAAAAATCAATGAAGAGAT+CGG + chr4.2:80062103-80062122 MS.gene066657:CDS 20.0%
!!! TAAGAAATCCAAATTTTGTT+AGG + chr4.2:80062425-80062444 MS.gene066657:CDS 20.0%
! AAAAATGAATCGACAATTAG+AGG - chr4.2:80061710-80061729 None:intergenic 25.0%
! CTATTAAATACTTCTTCTTG+TGG - chr4.2:80062205-80062224 None:intergenic 25.0%
! GTATGGTTAAAATGAAAGTT+GGG + chr4.2:80062299-80062318 MS.gene066657:CDS 25.0%
! TATGGTTAAAATGAAAGTTG+GGG + chr4.2:80062300-80062319 MS.gene066657:CDS 25.0%
! TGTATGGTTAAAATGAAAGT+TGG + chr4.2:80062298-80062317 MS.gene066657:CDS 25.0%
!! ATAAAGAAGACCCTTTTTAT+TGG - chr4.2:80061471-80061490 None:intergenic 25.0%
!! ATAGTTTGATCACTTGATTT+TGG - chr4.2:80061407-80061426 None:intergenic 25.0%
!! CCATTACCAGAAAATTATTT+TGG + chr4.2:80062259-80062278 MS.gene066657:CDS 25.0%
!! GCATTTCCAAAATAATTTTC+TGG - chr4.2:80062268-80062287 None:intergenic 25.0%
!! TATACTACCTTTTTCTATTC+CGG - chr4.2:80062590-80062609 None:intergenic 25.0%
!!! AAATAATTTTCTGGTAATGG+TGG - chr4.2:80062259-80062278 None:intergenic 25.0%
!!! CCAAAATAATTTTCTGGTAA+TGG - chr4.2:80062262-80062281 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + chr4.2:80062502-80062521 MS.gene066657:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG + chr4.2:80062507-80062526 MS.gene066657:CDS 25.0%
!!! TTGTAGAGAAGATATTTTGA+TGG - chr4.2:80062131-80062150 None:intergenic 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG + chr4.2:80062506-80062525 MS.gene066657:CDS 25.0%
AAAGTGAAAGGAAATCGTAT+TGG - chr4.2:80061968-80061987 None:intergenic 30.0%
AACAAAAGAATCACTCTGAT+GGG + chr4.2:80061993-80062012 MS.gene066657:CDS 30.0%
AATACGATTTCCTTTCACTT+TGG + chr4.2:80061967-80061986 MS.gene066657:CDS 30.0%
ACACAACATTCATGAATTGA+GGG - chr4.2:80062663-80062682 None:intergenic 30.0%
ACTTGCTTCAACCAATAAAA+AGG + chr4.2:80061457-80061476 MS.gene066657:CDS 30.0%
ATGAATTGATGAAATGCCAT+AGG - chr4.2:80061859-80061878 None:intergenic 30.0%
ATGGCATTTCATCAATTCAT+GGG + chr4.2:80061859-80061878 MS.gene066657:CDS 30.0%
CTTGCTTCAACCAATAAAAA+GGG + chr4.2:80061458-80061477 MS.gene066657:CDS 30.0%
GTATTTAATAGAGTCGTGAT+AGG + chr4.2:80062214-80062233 MS.gene066657:CDS 30.0%
TATGGCATTTCATCAATTCA+TGG + chr4.2:80061858-80061877 MS.gene066657:CDS 30.0%
TGAATTGATGAAATGCCATA+GGG - chr4.2:80061858-80061877 None:intergenic 30.0%
TTACCATAAACATCAAACCA+TGG - chr4.2:80062496-80062515 None:intergenic 30.0%
TTAGCAAGCCTAACAAAATT+TGG - chr4.2:80062436-80062455 None:intergenic 30.0%
TTCTTGTGGATCAAATTGTT+TGG - chr4.2:80062191-80062210 None:intergenic 30.0%
!! AAACTATTGATTTGACTCCA+TGG + chr4.2:80061420-80061439 MS.gene066657:CDS 30.0%
!! AACTATTGATTTGACTCCAT+GGG + chr4.2:80061421-80061440 MS.gene066657:CDS 30.0%
!! TATGGTAATGATTTTGGATG+GGG + chr4.2:80062508-80062527 MS.gene066657:CDS 30.0%
AACAAAGTTAGAAGCTACGA+AGG + chr4.2:80061737-80061756 MS.gene066657:CDS 35.0%
ACCTGCATTGTTGCATATAA+CGG - chr4.2:80061621-80061640 None:intergenic 35.0%
CAACAAAAGAATCACTCTGA+TGG + chr4.2:80061992-80062011 MS.gene066657:CDS 35.0%
GACACAACATTCATGAATTG+AGG - chr4.2:80062664-80062683 None:intergenic 35.0%
GTGATTCTTCAACTTCTCAT+CGG - chr4.2:80062392-80062411 None:intergenic 35.0%
TCTCCATGGTTTGATGTTTA+TGG + chr4.2:80062490-80062509 MS.gene066657:CDS 35.0%
TGGTTGAAGCAAGTAGAAAT+CGG - chr4.2:80061451-80061470 None:intergenic 35.0%
TTGAAGCTAACCAAATCCAA+CGG + chr4.2:80061501-80061520 MS.gene066657:CDS 35.0%
TTTAACCATGTTGTCGTTGA+CGG + chr4.2:80061827-80061846 MS.gene066657:CDS 35.0%
! ACAATTAGAGGTACATAGGT+GGG - chr4.2:80061698-80061717 None:intergenic 35.0%
! ATCGACAATTAGAGGTACAT+AGG - chr4.2:80061702-80061721 None:intergenic 35.0%
! TTTTGTTGCTCCAAAGTGAA+AGG - chr4.2:80061980-80061999 None:intergenic 35.0%
!! AAGCGCTTTTAACTCATCTT+TGG + chr4.2:80062149-80062168 MS.gene066657:CDS 35.0%
!! CGTAGTGGATATAAAGCAAA+TGG + chr4.2:80062547-80062566 MS.gene066657:CDS 35.0%
!! TTTAACTCATCTTTGGTGCT+CGG + chr4.2:80062156-80062175 MS.gene066657:CDS 35.0%
!!! AGTGTTCCGTTATTTTGAGA+CGG - chr4.2:80061577-80061596 None:intergenic 35.0%
!!! TTTTGTTAGGCTTGCTAACA+TGG + chr4.2:80062438-80062457 MS.gene066657:CDS 35.0%
AAACCATGGAGAACTGCTAA+TGG - chr4.2:80062482-80062501 None:intergenic 40.0%
AATAGAGTCGTGATAGGAGT+TGG + chr4.2:80062220-80062239 MS.gene066657:CDS 40.0%
AATGAAAGTTGGGGAGTTGT+TGG + chr4.2:80062309-80062328 MS.gene066657:CDS 40.0%
AGGCACATCAAAACCATTGT+TGG + chr4.2:80061757-80061776 MS.gene066657:CDS 40.0%
ATTGTTGGCGGTTCAAGTAA+CGG + chr4.2:80061772-80061791 MS.gene066657:CDS 40.0%
CACATCAAAACCATTGTTGG+CGG + chr4.2:80061760-80061779 MS.gene066657:CDS 40.0%
CATGTGGAAGACAAACTTGA+AGG - chr4.2:80062615-80062634 None:intergenic 40.0%
CTAGTTGACGGTATGTTCAT+TGG + chr4.2:80061797-80061816 MS.gene066657:CDS 40.0%
GTGTTTGCCGGAATAGAAAA+AGG + chr4.2:80062580-80062599 MS.gene066657:CDS 40.0%
TATCCATTAGCAGTTCTCCA+TGG + chr4.2:80062476-80062495 MS.gene066657:CDS 40.0%
TCCGTTATATGCAACAATGC+AGG + chr4.2:80061617-80061636 MS.gene066657:CDS 40.0%
TCTCTCCACTGATACAATCA+AGG + chr4.2:80061379-80061398 MS.gene066657:CDS 40.0%
TGAAGAATCACTACGAGTCT+TGG + chr4.2:80062401-80062420 MS.gene066657:CDS 40.0%
TGTTGGCCGTCTCAAAATAA+CGG + chr4.2:80061568-80061587 MS.gene066657:CDS 40.0%
TTGGAAAAAGGTAAGAGCAG+AGG - chr4.2:80061543-80061562 None:intergenic 40.0%
TTGTCTTCCACATGAGATTC+TGG + chr4.2:80062621-80062640 MS.gene066657:CDS 40.0%
! ACCTTTTTCCAACCTCTTGT+TGG + chr4.2:80061551-80061570 MS.gene066657:CDS 40.0%
! CAAGTAACGGAACTAGTTGA+CGG + chr4.2:80061785-80061804 MS.gene066657:CDS 40.0%
! GACAATTAGAGGTACATAGG+TGG - chr4.2:80061699-80061718 None:intergenic 40.0%
!! TGGATTTGGTTAGCTTCAAC+TGG - chr4.2:80061500-80061519 None:intergenic 40.0%
!! TTTGGTTAGCTTCAACTGGA+TGG - chr4.2:80061496-80061515 None:intergenic 40.0%
AAACCAACGCTCTAGTGTTG+GGG - chr4.2:80061927-80061946 None:intergenic 45.0%
AAGTATTCTCAGCTGCAGCA+TGG - chr4.2:80061655-80061674 None:intergenic 45.0%
ACTTTCCGTCAACGACAACA+TGG - chr4.2:80061835-80061854 None:intergenic 45.0%
AGTAGAAATCGGAGATCCCA+TGG - chr4.2:80061440-80061459 None:intergenic 45.0%
ATCAGTGGAGAGAACTCGTA+CGG - chr4.2:80061372-80061391 None:intergenic 45.0%
ATTGCCTCCAGAATCTCATG+TGG - chr4.2:80062631-80062650 None:intergenic 45.0%
ATTGGCTGTTGAACACCATC+CGG - chr4.2:80061950-80061969 None:intergenic 45.0%
GAAACCAACGCTCTAGTGTT+GGG - chr4.2:80061928-80061947 None:intergenic 45.0%
GCCAACAAGAGGTTGGAAAA+AGG - chr4.2:80061555-80061574 None:intergenic 45.0%
GTTACTTGAACCGCCAACAA+TGG - chr4.2:80061773-80061792 None:intergenic 45.0%
TATCCACTACGAACTGCTAC+AGG - chr4.2:80062538-80062557 None:intergenic 45.0%
TCTTCCACATGAGATTCTGG+AGG + chr4.2:80062624-80062643 MS.gene066657:CDS 45.0%
TGGTGCCTTGATTGTATCAG+TGG - chr4.2:80061387-80061406 None:intergenic 45.0%
! ACATGAGATTCTGGAGGCAA+TGG + chr4.2:80062630-80062649 MS.gene066657:CDS 45.0%
! GAGGAAGGTGGACTTTGTAA+AGG + chr4.2:80062331-80062350 MS.gene066657:CDS 45.0%
! GGTAATGGTGGAACTAACCT+TGG - chr4.2:80062247-80062266 None:intergenic 45.0%
!! AACACTAGAGCGTTGGTTTC+CGG + chr4.2:80061928-80061947 MS.gene066657:CDS 45.0%
!! GGACTTGTTACAGTGTTTGC+CGG + chr4.2:80062568-80062587 MS.gene066657:CDS 45.0%
!!! ATTTTGAGACGGCCAACAAG+AGG - chr4.2:80061566-80061585 None:intergenic 45.0%
AAGCCTGTAGCAGTTCGTAG+TGG + chr4.2:80062532-80062551 MS.gene066657:CDS 50.0%
AGTGTCTCAGCCGTTGGATT+TGG - chr4.2:80061514-80061533 None:intergenic 50.0%
GAAAGTTGGGGAGTTGTTGG+AGG + chr4.2:80062312-80062331 MS.gene066657:CDS 50.0%
GGAAACCAACGCTCTAGTGT+TGG - chr4.2:80061929-80061948 None:intergenic 50.0%
TCGTGATAGGAGTTGGACCA+AGG + chr4.2:80062227-80062246 MS.gene066657:CDS 50.0%
TCGTTGACGGAAAGTCCCTA+TGG + chr4.2:80061840-80061859 MS.gene066657:CDS 50.0%
! AACCAACGCTCTAGTGTTGG+GGG - chr4.2:80061926-80061945 None:intergenic 50.0%
! GAGGTACATAGGTGGGTTCT+AGG - chr4.2:80061691-80061710 None:intergenic 50.0%
AACCCCCAACACTAGAGCGT+TGG + chr4.2:80061921-80061940 MS.gene066657:CDS 55.0%
GTTGGGGAGTTGTTGGAGGA+AGG + chr4.2:80062316-80062335 MS.gene066657:CDS 55.0%
TGAGACGGCCAACAAGAGGT+TGG - chr4.2:80061562-80061581 None:intergenic 55.0%
!! ACAGAGAGTGTCTCAGCCGT+TGG - chr4.2:80061520-80061539 None:intergenic 55.0%
!! CTAGAGCGTTGGTTTCCGGA+TGG + chr4.2:80061932-80061951 MS.gene066657:CDS 55.0%
GGGGAGTTGTTGGAGGAAGG+TGG + chr4.2:80062319-80062338 MS.gene066657:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 80061362 80062690 80061362 ID=MS.gene066657
chr4.2 mRNA 80061362 80062690 80061362 ID=MS.gene066657.t1;Parent=MS.gene066657
chr4.2 exon 80061362 80062690 80061362 ID=MS.gene066657.t1.exon1;Parent=MS.gene066657.t1
chr4.2 CDS 80061362 80062690 80061362 ID=cds.MS.gene066657.t1;Parent=MS.gene066657.t1
Gene Sequence

>MS.gene066657

ATGGGAACCGTACGAGTTCTCTCCACTGATACAATCAAGGCACCAAAATCAAGTGATCAAACTATTGATTTGACTCCATGGGATCTCCGATTTCTACTTGCTTCAACCAATAAAAAGGGTCTTCTTTATCACCATCCAGTTGAAGCTAACCAAATCCAACGGCTGAGACACTCTCTGTCCTCTGCTCTTACCTTTTTCCAACCTCTTGTTGGCCGTCTCAAAATAACGGAACACTCAGACAACACTGTCTCGTGTTCCGTTATATGCAACAATGCAGGTGTACTCTTTATCCATGCTGCAGCTGAGAATACTTGTGTTGCTGACATCCTAGAACCCACCTATGTACCTCTAATTGTCGATTCATTTTTTGTTTTAAACAAAGTTAGAAGCTACGAAGGCACATCAAAACCATTGTTGGCGGTTCAAGTAACGGAACTAGTTGACGGTATGTTCATTGGTTGCTCATTTAACCATGTTGTCGTTGACGGAAAGTCCCTATGGCATTTCATCAATTCATGGGCTGAAATTTCACGTAGTTGTTGTCATCATCAAATATCTAAACCCCCAACACTAGAGCGTTGGTTTCCGGATGGTGTTCAACAGCCAATACGATTTCCTTTCACTTTGGAGCAACAAAAGAATCACTCTGATGGGTTGAGTTTTTCGTCGTTTGATGATGAAAAACTTTGTTTATCAAATAGGGTATTTCATTTCACAAAAGAGAAAATTGTGCAGCTGAAATTAAAAATCAATGAAGAGATCGGTACCATCAAAATATCTTCTCTACAAGCGCTTTTAACTCATCTTTGGTGCTCGGTGATGCGTTCCAAACAATTTGATCCACAAGAAGAAGTATTTAATAGAGTCGTGATAGGAGTTGGACCAAGGTTAGTTCCACCATTACCAGAAAATTATTTTGGAAATGCAATTATAAGTTGTATGGTTAAAATGAAAGTTGGGGAGTTGTTGGAGGAAGGTGGACTTTGTAAAGGTGCTTGCGAGATGAACAAGTTAATTGCTTCACACACCGATGAGAAGTTGAAGAATCACTACGAGTCTTGGTTAAGAAATCCAAATTTTGTTAGGCTTGCTAACATGGTAAAAAATAATTTCATATCCATTAGCAGTTCTCCATGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAGCCTGTAGCAGTTCGTAGTGGATATAAAGCAAATGGACTTGTTACAGTGTTTGCCGGAATAGAAAAAGGTAGTATAGACCTTCAAGTTTGTCTTCCACATGAGATTCTGGAGGCAATGGAAAATGACCCTCAATTCATGAATGTTGTGTCCAACTAA
Protein sequence

>MS.gene066657.t1

MGTVRVLSTDTIKAPKSSDQTIDLTPWDLRFLLASTNKKGLLYHHPVEANQIQRLRHSLSSALTFFQPLVGRLKITEHSDNTVSCSVICNNAGVLFIHAAAENTCVADILEPTYVPLIVDSFFVLNKVRSYEGTSKPLLAVQVTELVDGMFIGCSFNHVVVDGKSLWHFINSWAEISRSCCHHQISKPPTLERWFPDGVQQPIRFPFTLEQQKNHSDGLSFSSFDDEKLCLSNRVFHFTKEKIVQLKLKINEEIGTIKISSLQALLTHLWCSVMRSKQFDPQEEVFNRVVIGVGPRLVPPLPENYFGNAIISCMVKMKVGELLEEGGLCKGACEMNKLIASHTDEKLKNHYESWLRNPNFVRLANMVKNNFISISSSPWFDVYGNDFGWGKPVAVRSGYKANGLVTVFAGIEKGSIDLQVCLPHEILEAMENDPQFMNVVSN