AlfalfaGEDB | MS.gene008574

M. sativa cultivar XinJiangDaYe / MS.gene008574

KEGG: K20240
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008574.t1	XP_013460366.1	92.1	471	33	3	1	470	1	468	6.30E-251	876.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008574.t1	O80467	39.7	458	257	9	10	466	11	450	2.7e-77	290.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008574.t1	A0A072UX85	92.1	471	33	3	1	470	1	468	4.5e-251	876.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene008574	MS.gene38361	PPI
MS.gene008574	MS.gene063865	PPI
MS.gene008574	MS.gene50356	PPI
MS.gene008574	MS.gene56797	PPI
MS.gene008574	MS.gene032349	PPI
MS.gene008574	MS.gene011837	PPI
MS.gene008574	MS.gene069767	PPI
MS.gene008574	MS.gene38363	PPI
MS.gene50379	MS.gene008574	PPI
MS.gene008574	MS.gene78818	PPI
MS.gene008574	MS.gene28251	PPI
MS.gene008574	MS.gene90491	PPI
MS.gene008574	MS.gene32936	PPI
MS.gene008574	MS.gene38359	PPI
MS.gene008574	MS.gene016532	PPI
MS.gene008574	MS.gene066701	PPI
MS.gene008574	MS.gene50355	PPI
MS.gene008574	MS.gene039597	PPI
MS.gene066634	MS.gene008574	PPI
MS.gene008574	MS.gene011799	PPI
MS.gene008574	MS.gene002544	PPI
MS.gene008574	MS.gene50420	PPI
MS.gene008574	MS.gene32933	PPI
MS.gene008574	MS.gene46265	PPI
MS.gene008574	MS.gene064101	PPI
MS.gene008574	MS.gene90496	PPI
MS.gene008574	MS.gene87536	PPI
MS.gene008574	MS.gene99218	PPI
MS.gene008574	MS.gene38706	PPI
MS.gene011796	MS.gene008574	PPI
MS.gene87537	MS.gene008574	PPI
MS.gene008574	MS.gene004218	PPI
MS.gene008574	MS.gene38701	PPI
MS.gene008574	MS.gene016477	PPI
MS.gene013787	MS.gene008574	PPI
MS.gene008574	MS.gene012960	PPI
MS.gene008574	MS.gene02573	PPI
MS.gene008574	MS.gene011798	PPI
MS.gene87532	MS.gene008574	PPI
MS.gene99223	MS.gene008574	PPI
MS.gene008574	MS.gene012957	PPI
MS.gene008574	MS.gene87577	PPI
MS.gene008574	MS.gene38699	PPI
MS.gene008574	MS.gene95242	PPI
MS.gene008574	MS.gene000764	PPI
MS.gene008574	MS.gene50382	PPI
MS.gene007831	MS.gene008574	PPI
MS.gene008574	MS.gene56798	PPI
MS.gene008574	MS.gene38704	PPI
MS.gene008574	MS.gene012959	PPI
MS.gene008574	MS.gene87191	PPI
MS.gene008574	MS.gene56796	PPI
MS.gene99222	MS.gene008574	PPI
MS.gene008574	MS.gene87496	PPI
MS.gene008574	MS.gene070019	PPI
MS.gene066656	MS.gene008574	PPI
MS.gene008574	MS.gene011801	PPI
MS.gene008574	MS.gene90493	PPI
MS.gene066660	MS.gene008574	PPI
MS.gene008574	MS.gene066657	PPI
MS.gene008574	MS.gene32938	PPI
MS.gene008574	MS.gene56799	PPI
MS.gene008574	MS.gene063868	PPI
MS.gene03202	MS.gene008574	PPI
MS.gene008574	MS.gene069321	PPI
MS.gene008574	MS.gene000763	PPI
MS.gene008574	MS.gene38702	PPI
MS.gene008574	MS.gene32934	PPI
MS.gene008574	MS.gene012965	PPI
MS.gene008574	MS.gene90492	PPI
MS.gene046246	MS.gene008574	PPI
MS.gene38700	MS.gene008574	PPI
MS.gene008574	MS.gene50384	PPI
MS.gene008574	MS.gene38977	PPI
MS.gene008574	MS.gene38703	PPI
MS.gene008574	MS.gene066658	PPI
MS.gene32261	MS.gene008574	PPI
MS.gene008574	MS.gene38365	PPI
MS.gene008574	MS.gene012961	PPI
MS.gene99219	MS.gene008574	PPI
MS.gene008574	MS.gene38362	PPI
MS.gene56795	MS.gene008574	PPI
MS.gene008574	MS.gene99221	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008574.t1	MTR_3g465880	92.144	471	33	3	1	470	1	468	0.0	899
MS.gene008574.t1	MTR_3g465930	67.722	474	138	9	1	469	1	464	0.0	644
MS.gene008574.t1	MTR_3g465910	67.660	470	136	9	1	466	1	458	0.0	641
MS.gene008574.t1	MTR_3g465980	66.030	471	147	7	1	469	1	460	0.0	629
MS.gene008574.t1	MTR_3g465990	65.817	471	148	7	1	469	1	460	0.0	628
MS.gene008574.t1	MTR_3g465950	68.202	456	126	7	1	454	1	439	0.0	613
MS.gene008574.t1	MTR_3g465870	62.076	472	131	8	1	470	1	426	0.0	565
MS.gene008574.t1	MTR_6g053810	60.907	463	172	7	9	468	8	464	0.0	560
MS.gene008574.t1	MTR_4g055170	51.374	473	206	12	3	469	4	458	5.02e-154	446
MS.gene008574.t1	MTR_2g015430	31.236	461	282	15	12	464	2	435	1.74e-58	199
MS.gene008574.t1	MTR_8g096530	28.541	466	279	17	10	464	5	427	8.68e-36	138
MS.gene008574.t1	MTR_1g053315	28.163	490	274	26	7	463	13	457	2.46e-34	134
MS.gene008574.t1	MTR_1g081660	28.009	457	286	16	19	466	25	447	2.87e-34	134
MS.gene008574.t1	MTR_7g027175	31.204	407	248	16	8	409	13	392	3.64e-34	133
MS.gene008574.t1	MTR_1g040175	30.878	353	216	11	8	352	5	337	7.36e-34	132
MS.gene008574.t1	MTR_7g114520	27.521	476	293	16	6	466	5	443	3.33e-33	131
MS.gene008574.t1	MTR_3g071320	28.228	457	290	14	12	461	5	430	6.04e-31	124
MS.gene008574.t1	MTR_3g098980	27.753	454	275	19	18	461	20	430	3.31e-30	122
MS.gene008574.t1	MTR_1g040215	26.392	485	259	16	19	466	16	439	4.21e-30	122
MS.gene008574.t1	MTR_7g110610	30.312	353	173	12	19	362	12	300	1.94e-29	119
MS.gene008574.t1	MTR_7g114550	27.475	404	249	13	19	409	17	389	8.13e-29	118
MS.gene008574.t1	MTR_5g076700	26.316	418	263	13	6	409	4	390	1.74e-28	117
MS.gene008574.t1	MTR_1g040320	26.840	462	272	14	8	466	5	403	6.57e-28	115
MS.gene008574.t1	MTR_7g114470	25.847	472	302	14	6	464	5	441	1.06e-27	115
MS.gene008574.t1	MTR_7g114490	27.163	416	261	13	6	409	4	389	6.93e-26	110
MS.gene008574.t1	MTR_7g114560	26.570	414	266	13	6	409	4	389	1.29e-25	109
MS.gene008574.t1	MTR_1g040195	27.753	454	267	15	19	466	16	414	6.93e-24	103
MS.gene008574.t1	MTR_1g040315	29.524	315	186	10	19	323	16	304	1.24e-23	103
MS.gene008574.t1	MTR_3g112180	25.000	456	281	15	20	460	20	429	2.73e-23	102
MS.gene008574.t1	MTR_3g026950	25.348	359	240	12	20	366	10	352	2.87e-23	102
MS.gene008574.t1	MTR_3g049150	27.371	369	228	15	14	366	4	348	3.10e-23	102
MS.gene008574.t1	MTR_8g075610	26.739	460	275	21	19	461	1	415	9.67e-23	100
MS.gene008574.t1	MTR_2g105330	25.380	461	297	18	12	461	3	427	4.21e-22	98.6
MS.gene008574.t1	MTR_2g064495	25.380	461	297	18	12	461	3	427	7.64e-22	97.8
MS.gene008574.t1	MTR_4g007540	25.325	462	297	18	12	461	3	428	8.29e-21	94.7
MS.gene008574.t1	MTR_7g085930	24.494	445	274	19	49	461	41	455	4.53e-19	90.1
MS.gene008574.t1	MTR_7g085990	26.420	352	215	17	49	376	37	368	1.28e-16	82.4
MS.gene008574.t1	MTR_0502s0010	28.685	251	145	8	113	352	11	238	1.74e-16	79.3
MS.gene008574.t1	MTR_4g127650	26.230	366	242	14	11	366	9	356	9.69e-16	79.7
MS.gene008574.t1	MTR_7g086000	27.151	372	227	16	20	366	12	364	1.90e-15	79.0
MS.gene008574.t1	MTR_7g084940	24.816	407	257	16	5	395	9	382	3.37e-15	77.8
MS.gene008574.t1	MTR_0042s0190	25.726	241	156	7	174	409	1	223	6.70e-13	69.7
MS.gene008574.t1	MTR_4g022825	25.347	288	187	7	65	338	50	323	1.31e-12	69.7
MS.gene008574.t1	MTR_5g090830	24.308	325	205	11	70	373	66	370	6.35e-12	67.8
MS.gene008574.t1	MTR_5g090880	23.810	441	262	17	70	470	67	473	2.43e-11	66.2
MS.gene008574.t1	MTR_3g450690	25.617	324	198	12	70	371	72	374	6.60e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008574.t1	AT5G07080	40.213	470	243	11	6	466	8	448	9.63e-99	304
MS.gene008574.t1	AT2G23510	40.222	450	248	11	19	466	20	450	7.45e-97	299
MS.gene008574.t1	AT2G25150	38.608	474	267	11	1	469	1	455	9.97e-92	286
MS.gene008574.t1	AT3G47170	37.580	471	261	14	12	466	12	465	6.92e-79	253
MS.gene008574.t1	AT1G28680	35.590	458	270	12	12	464	4	441	5.14e-76	245
MS.gene008574.t1	AT1G28680	36.745	381	221	10	12	387	4	369	8.08e-63	209
MS.gene008574.t1	AT5G41040	28.541	466	283	18	8	463	29	454	4.78e-40	150
MS.gene008574.t1	AT5G41040	28.541	466	283	18	8	463	13	438	5.07e-40	149
MS.gene008574.t1	AT3G62160	27.952	415	252	12	8	413	1	377	6.06e-39	146
MS.gene008574.t1	AT3G03480	29.247	465	292	15	8	466	15	448	1.44e-38	145
MS.gene008574.t1	AT5G17540	26.667	465	305	12	8	466	5	439	1.84e-31	126
MS.gene008574.t1	AT2G40230	29.454	421	262	14	12	422	5	400	6.13e-29	118
MS.gene008574.t1	AT3G48720	28.478	460	271	22	15	461	12	426	8.13e-29	118
MS.gene008574.t1	AT1G03390	26.754	456	281	22	8	441	13	437	1.03e-25	109
MS.gene008574.t1	AT5G63560	27.561	410	256	15	8	409	5	381	2.92e-25	108
MS.gene008574.t1	AT5G48930	27.523	436	260	21	10	417	1	408	4.16e-24	104
MS.gene008574.t1	AT1G27620	26.000	400	238	16	69	452	57	414	2.66e-21	96.7
MS.gene008574.t1	AT1G65450	28.571	413	254	17	2	397	4	392	4.28e-19	90.1
MS.gene008574.t1	AT4G31910	26.764	411	253	17	33	416	29	418	7.21e-18	86.3
MS.gene008574.t1	AT2G19070	25.986	431	243	19	69	463	57	447	9.92e-18	85.9
MS.gene008574.t1	AT1G24430	30.216	139	84	5	71	208	63	189	1.75e-11	66.2
MS.gene008574.t1	AT5G23940	26.894	264	170	10	71	322	66	318	1.75e-11	66.6

Find 94 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATTTCTAGCTTTAGGTTT+AGG	0.176003	3.3:+53943514	None:intergenic
CATTTCTAGCTTTAGGTTTA+GGG	0.192317	3.3:+53943515	None:intergenic
ATGCAACATGTTTGCGTCTT+TGG	0.207307	3.3:-53942120	MS.gene008574:CDS
GATGAAAATAGTATAGATTT+TGG	0.226632	3.3:-53942176	MS.gene008574:CDS
CTGACTGAGTGGAAGCATTT+AGG	0.231506	3.3:-53942206	MS.gene008574:CDS
CCTTTGCCTATAGGGTATTA+TGG	0.248309	3.3:-53942425	MS.gene008574:CDS
CCTTTCTTCTCTTCATTACT+TGG	0.251953	3.3:-53943005	MS.gene008574:CDS
AAGTTACCCTTTGCTATTCA+TGG	0.268633	3.3:-53942921	MS.gene008574:CDS
CATCTTTCATTTCTAGCTTT+AGG	0.271621	3.3:+53943508	None:intergenic
TTAACTCCTCCTTTCATTGA+TGG	0.282796	3.3:+53942050	None:intergenic
GATTATTATTATCCTCTTGC+AGG	0.285447	3.3:-53943300	MS.gene008574:intron
GTTATGATCCTTTGCCTATA+GGG	0.290354	3.3:-53942433	MS.gene008574:CDS
ACAATTCATGATTCTGATTC+GGG	0.295244	3.3:-53943369	MS.gene008574:CDS
GTTGATCAAAGAGACTAAAA+AGG	0.312335	3.3:-53942327	MS.gene008574:CDS
AACTTTGGCATGGCAGCAAC+AGG	0.315484	3.3:+53942011	None:intergenic
GGTTATGATCCTTTGCCTAT+AGG	0.333082	3.3:-53942434	MS.gene008574:CDS
AACAATTCATGATTCTGATT+CGG	0.342734	3.3:-53943370	MS.gene008574:CDS
GGAGTTGGTGGTGCAGAATA+TGG	0.344499	3.3:-53943061	MS.gene008574:CDS
GAAAATGCAACAGGTTCTTT+TGG	0.344870	3.3:+53942692	None:intergenic
CATGTTGTTTGTGATGGATA+TGG	0.348097	3.3:-53942851	MS.gene008574:CDS
ATTTGTGGTGGTTTCACAAT+TGG	0.352743	3.3:-53942887	MS.gene008574:CDS
TCAATTTCCTCCTTGAACTT+TGG	0.354566	3.3:+53941996	None:intergenic
AGAAGCACTCTCAAAGGCTT+TGG	0.364642	3.3:-53943322	MS.gene008574:CDS
TCCATTGGTGATAAGTTGTA+AGG	0.380173	3.3:+53942502	None:intergenic
TGGTGGAAGTGGTTGTTTGC+TGG	0.388443	3.3:+53942724	None:intergenic
GGTGGTTTCACAATTGGAAT+GGG	0.391685	3.3:-53942881	MS.gene008574:CDS
TGGTGGTTTCACAATTGGAA+TGG	0.410300	3.3:-53942882	MS.gene008574:CDS
AGTGACTCAAAAGTTGTAAC+TGG	0.415404	3.3:+53942557	None:intergenic
GACTCTAAGTCTTCCGTCTC+CGG	0.416343	3.3:+53943090	None:intergenic
TCAGAATCATGAATTGTTGA+TGG	0.437948	3.3:+53943375	None:intergenic
TTAGCATATGCTTCTAAGAA+TGG	0.443157	3.3:+53943034	None:intergenic
GCCTTCTGTGTTACCTGTGT+GGG	0.449032	3.3:-53942777	MS.gene008574:CDS
TGCTGCCATGCCAAAGTTCA+AGG	0.449608	3.3:-53942006	MS.gene008574:CDS
GCAATTGTTGAACTTGCAAA+AGG	0.451246	3.3:-53942809	MS.gene008574:CDS
GGAAGACTTAGAGTCGTGTT+TGG	0.459101	3.3:-53943082	MS.gene008574:CDS
TGTTCACATGTTGTTTGTGA+TGG	0.460683	3.3:-53942857	MS.gene008574:CDS
GTCAAGCTGTTCATGTATAC+CGG	0.467168	3.3:-53943398	MS.gene008574:CDS
TCATCATCATCAACTTTACT+AGG	0.476073	3.3:+53942077	None:intergenic
ACTTAAGATGAGTTTGATGA+AGG	0.481102	3.3:-53942600	MS.gene008574:CDS
GGTTGAAGTAATGGTGGAAG+TGG	0.490092	3.3:+53942713	None:intergenic
CCAAGTAATGAAGAGAAGAA+AGG	0.491172	3.3:+53943005	None:intergenic
ACTAAAGGTGAAAATGCAAC+AGG	0.491686	3.3:+53942683	None:intergenic
TCTTGCTTATGCTCCGTCTC+CGG	0.493846	3.3:+53942263	None:intergenic
CTTAGAGTCGTGTTTGGAGT+TGG	0.494597	3.3:-53943076	MS.gene008574:CDS
AAAAGCAATGTTGAATATGT+TGG	0.498010	3.3:-53942480	MS.gene008574:CDS
GTATTACCATAATACCCTAT+AGG	0.501660	3.3:+53942419	None:intergenic
AAGATGAGTTTGATGAAGGA+AGG	0.508475	3.3:-53942596	MS.gene008574:CDS
CAAGTAATGAAGAGAAGAAA+GGG	0.508480	3.3:+53943006	None:intergenic
AATACAAGAAGGAATGGACT+TGG	0.517455	3.3:+53943467	None:intergenic
AGAAAGAATACAAGAAGGAA+TGG	0.519099	3.3:+53943461	None:intergenic
TTGTCACCATGAATAGCAAA+GGG	0.521433	3.3:+53942915	None:intergenic
TGGGAAAGAGAGAGACTAGT+TGG	0.531305	3.3:-53942758	MS.gene008574:CDS
GGTATTAGACGCAGCATGAA+AGG	0.539149	3.3:-53942455	MS.gene008574:CDS
CACTTGGTGCTTATGTGTGG+AGG	0.540041	3.3:-53942538	MS.gene008574:CDS
TATGGTAATACTACTGTGGA+TGG	0.540481	3.3:-53942407	MS.gene008574:CDS
TTTGTCACCATGAATAGCAA+AGG	0.542722	3.3:+53942914	None:intergenic
ATTTCTAGCTTTAGGTTTAG+GGG	0.543168	3.3:+53943516	None:intergenic
AGAGTCGTGTTTGGAGTTGG+TGG	0.545215	3.3:-53943073	MS.gene008574:CDS
TTCCTCCTTGAACTTTGGCA+TGG	0.549243	3.3:+53942001	None:intergenic
GACAGAGAAAGAATACAAGA+AGG	0.550104	3.3:+53943456	None:intergenic
CTCGATCGACACTCCGGAGA+CGG	0.550133	3.3:-53942276	MS.gene008574:CDS
CACAGATATTGCAAAAGACT+TGG	0.550145	3.3:-53942972	MS.gene008574:CDS
AGTCACTTGGTGCTTATGTG+TGG	0.550371	3.3:-53942541	MS.gene008574:CDS
GCCTTACAACTTATCACCAA+TGG	0.553004	3.3:-53942503	MS.gene008574:CDS
AACTTAACAATTTCATAGAG+TGG	0.558390	3.3:+53942347	None:intergenic
AACATGTTTGCGTCTTTGGA+AGG	0.558593	3.3:-53942116	MS.gene008574:CDS
AATCATGAATTGTTGATGGC+CGG	0.563884	3.3:+53943379	None:intergenic
CAAGAAGGAATGGACTTGGA+TGG	0.571444	3.3:+53943471	None:intergenic
TTTACAAGTATACCTGCAAG+AGG	0.571753	3.3:+53943288	None:intergenic
GTGACAAAATTTATTTGTGG+TGG	0.581717	3.3:-53942899	MS.gene008574:CDS
GCAAACATGTTGCATGGTGC+TGG	0.589020	3.3:+53942128	None:intergenic
CCATAATACCCTATAGGCAA+AGG	0.590841	3.3:+53942425	None:intergenic
GTGGTTTCACAATTGGAATG+GGG	0.594042	3.3:-53942880	MS.gene008574:CDS
AGCCTTCTGTGTTACCTGTG+TGG	0.596158	3.3:-53942778	MS.gene008574:CDS
ATTCAAAGAAGCACTCTCAA+AGG	0.600716	3.3:-53943328	MS.gene008574:CDS
ACATGTTTGCGTCTTTGGAA+GGG	0.601109	3.3:-53942115	MS.gene008574:CDS
ATGGTGACAAAATTTATTTG+TGG	0.603646	3.3:-53942902	MS.gene008574:CDS
GGTAGACTAGTGAGACATGC+CGG	0.607006	3.3:-53943109	MS.gene008574:CDS
GCAATGTTGAATATGTTGGT+TGG	0.607643	3.3:-53942476	MS.gene008574:CDS
CTAGTGAGACATGCCGGAGA+CGG	0.615424	3.3:-53943103	MS.gene008574:CDS
GATGATGATCCATCAATGAA+AGG	0.624021	3.3:-53942059	MS.gene008574:CDS
TCCCACACAGGTAACACAGA+AGG	0.627307	3.3:+53942776	None:intergenic
GCTTCTTTGAATACATGACA+AGG	0.632259	3.3:+53943339	None:intergenic
AAAGACGCAAACATGTTGCA+TGG	0.635230	3.3:+53942122	None:intergenic
CGGTAACGATTCTGACTGAG+TGG	0.635573	3.3:-53942217	MS.gene008574:CDS
GATGATCCATCAATGAAAGG+AGG	0.655138	3.3:-53942056	MS.gene008574:CDS
AGTCTCTCTCTTTCCCACAC+AGG	0.657387	3.3:+53942764	None:intergenic
ACTTGGTGCTTATGTGTGGA+GGG	0.659872	3.3:-53942537	MS.gene008574:CDS
TAGCATGGACGGTAGCGGTG+CGG	0.664954	3.3:-53942237	MS.gene008574:CDS
GTATTATGGTAATACTACTG+TGG	0.668892	3.3:-53942411	MS.gene008574:CDS
CTTCTTTGAATACATGACAA+GGG	0.672661	3.3:+53943340	None:intergenic
ATACATGAACAGCTTGACAA+AGG	0.673504	3.3:+53943403	None:intergenic
ACTTTGGCATGGCAGCAACA+GGG	0.676516	3.3:+53942012	None:intergenic
TGCCATGCCAAAGTTCAAGG+AGG	0.740408	3.3:-53942003	MS.gene008574:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATTAAAATCTATTGTTTTA+AGG	-	chr3.3:53942863-53942882	MS.gene008574:CDS	10.0%
!!!	TAGATTTTAATAACTGTTTT+TGG	+	chr3.3:53942855-53942874	None:intergenic	15.0%
!!!	AAAATAGTATAGATTTTGGA+GGG	-	chr3.3:53943336-53943355	MS.gene008574:CDS	20.0%
!!!	GATGAAAATAGTATAGATTT+TGG	-	chr3.3:53943332-53943351	MS.gene008574:CDS	20.0%
!	AAAAGCAATGTTGAATATGT+TGG	-	chr3.3:53943028-53943047	MS.gene008574:CDS	25.0%
!	AACAATTCATGATTCTGATT+CGG	-	chr3.3:53942138-53942157	MS.gene008574:CDS	25.0%
!	AACTTAACAATTTCATAGAG+TGG	+	chr3.3:53943164-53943183	None:intergenic	25.0%
!	ATAAATAAACAAACGGTGTT+TGG	+	chr3.3:53942309-53942328	None:intergenic	25.0%
!	ATGGTGACAAAATTTATTTG+TGG	-	chr3.3:53942606-53942625	MS.gene008574:CDS	25.0%
!!	GAAAATAGTATAGATTTTGG+AGG	-	chr3.3:53943335-53943354	MS.gene008574:CDS	25.0%
	ACAATTCATGATTCTGATTC+GGG	-	chr3.3:53942139-53942158	MS.gene008574:CDS	30.0%
	ACTTAAGATGAGTTTGATGA+AGG	-	chr3.3:53942908-53942927	MS.gene008574:CDS	30.0%
	AGAAAGAATACAAGAAGGAA+TGG	+	chr3.3:53942050-53942069	None:intergenic	30.0%
	CAAGTAATGAAGAGAAGAAA+GGG	+	chr3.3:53942505-53942524	None:intergenic	30.0%
	CTTCTTTGAATACATGACAA+GGG	+	chr3.3:53942171-53942190	None:intergenic	30.0%
	GATTATTATTATCCTCTTGC+AGG	-	chr3.3:53942208-53942227	MS.gene008574:CDS	30.0%
	GTATTACCATAATACCCTAT+AGG	+	chr3.3:53943092-53943111	None:intergenic	30.0%
	GTATTATGGTAATACTACTG+TGG	-	chr3.3:53943097-53943116	MS.gene008574:CDS	30.0%
	GTGACAAAATTTATTTGTGG+TGG	-	chr3.3:53942609-53942628	MS.gene008574:CDS	30.0%
	GTTGATCAAAGAGACTAAAA+AGG	-	chr3.3:53943181-53943200	MS.gene008574:intron	30.0%
	TCAGAATCATGAATTGTTGA+TGG	+	chr3.3:53942136-53942155	None:intergenic	30.0%
	TCATCATCATCAACTTTACT+AGG	+	chr3.3:53943434-53943453	None:intergenic	30.0%
	TTTAACGAGACATTCTTCTA+TGG	+	chr3.3:53942348-53942367	None:intergenic	30.0%
!	AAAGGGTAACTTTTATCTTG+AGG	+	chr3.3:53942579-53942598	None:intergenic	30.0%
!	TAACTTTTATCTTGAGGAGA+AGG	+	chr3.3:53942573-53942592	None:intergenic	30.0%
!	TTAGCATATGCTTCTAAGAA+TGG	+	chr3.3:53942477-53942496	None:intergenic	30.0%
!!	ATTCAACATTGCTTTTCCAT+TGG	+	chr3.3:53943024-53943043	None:intergenic	30.0%
!!	ATTTCTAGCTTTAGGTTTAG+GGG	+	chr3.3:53941995-53942014	None:intergenic	30.0%
!!	CATCTTTCATTTCTAGCTTT+AGG	+	chr3.3:53942003-53942022	None:intergenic	30.0%
!!	CATTTCTAGCTTTAGGTTTA+GGG	+	chr3.3:53941996-53942015	None:intergenic	30.0%
!!	TCATTTCTAGCTTTAGGTTT+AGG	+	chr3.3:53941997-53942016	None:intergenic	30.0%
!!!	ACTGTTTTTGGTTGAACTAA+AGG	+	chr3.3:53942843-53942862	None:intergenic	30.0%
	AAGATGAGTTTGATGAAGGA+AGG	-	chr3.3:53942912-53942931	MS.gene008574:CDS	35.0%
	ACTAAAGGTGAAAATGCAAC+AGG	+	chr3.3:53942828-53942847	None:intergenic	35.0%
	AGTGACTCAAAAGTTGTAAC+TGG	+	chr3.3:53942954-53942973	None:intergenic	35.0%
	ATACATGAACAGCTTGACAA+AGG	+	chr3.3:53942108-53942127	None:intergenic	35.0%
	ATTTGTGGTGGTTTCACAAT+TGG	-	chr3.3:53942621-53942640	MS.gene008574:CDS	35.0%
	CACAGATATTGCAAAAGACT+TGG	-	chr3.3:53942536-53942555	MS.gene008574:CDS	35.0%
	CCAAGTAATGAAGAGAAGAA+AGG	+	chr3.3:53942506-53942525	None:intergenic	35.0%
	CCTTTCTTCTCTTCATTACT+TGG	-	chr3.3:53942503-53942522	MS.gene008574:CDS	35.0%
	GACAGAGAAAGAATACAAGA+AGG	+	chr3.3:53942055-53942074	None:intergenic	35.0%
	GATGATGATCCATCAATGAA+AGG	-	chr3.3:53943449-53943468	MS.gene008574:CDS	35.0%
	GCAATGTTGAATATGTTGGT+TGG	-	chr3.3:53943032-53943051	MS.gene008574:CDS	35.0%
	GCAATTGTTGAACTTGCAAA+AGG	-	chr3.3:53942699-53942718	MS.gene008574:CDS	35.0%
	GCTTCTTTGAATACATGACA+AGG	+	chr3.3:53942172-53942191	None:intergenic	35.0%
	GTTATGATCCTTTGCCTATA+GGG	-	chr3.3:53943075-53943094	MS.gene008574:CDS	35.0%
	TATGGTAATACTACTGTGGA+TGG	-	chr3.3:53943101-53943120	MS.gene008574:CDS	35.0%
	TCAATTTCCTCCTTGAACTT+TGG	+	chr3.3:53943515-53943534	None:intergenic	35.0%
	TTAACTCCTCCTTTCATTGA+TGG	+	chr3.3:53943461-53943480	None:intergenic	35.0%
	TTGTCACCATGAATAGCAAA+GGG	+	chr3.3:53942596-53942615	None:intergenic	35.0%
	TTTACAAGTATACCTGCAAG+AGG	+	chr3.3:53942223-53942242	None:intergenic	35.0%
	TTTGTCACCATGAATAGCAA+AGG	+	chr3.3:53942597-53942616	None:intergenic	35.0%
!	AATACAAGAAGGAATGGACT+TGG	+	chr3.3:53942044-53942063	None:intergenic	35.0%
!	GAAAATGCAACAGGTTCTTT+TGG	+	chr3.3:53942819-53942838	None:intergenic	35.0%
!	TCCATTGGTGATAAGTTGTA+AGG	+	chr3.3:53943009-53943028	None:intergenic	35.0%
!	TGTTCACATGTTGTTTGTGA+TGG	-	chr3.3:53942651-53942670	MS.gene008574:CDS	35.0%
!!	AAGCAAGATTTTAGCATGGA+CGG	-	chr3.3:53943260-53943279	MS.gene008574:intron	35.0%
!!	AAGTTACCCTTTGCTATTCA+TGG	-	chr3.3:53942587-53942606	MS.gene008574:CDS	35.0%
!!	AATCATGAATTGTTGATGGC+CGG	+	chr3.3:53942132-53942151	None:intergenic	35.0%
!!	ATTCAAAGAAGCACTCTCAA+AGG	-	chr3.3:53942180-53942199	MS.gene008574:CDS	35.0%
!!	CATGTTGTTTGTGATGGATA+TGG	-	chr3.3:53942657-53942676	MS.gene008574:CDS	35.0%
!!	GCATAAGCAAGATTTTAGCA+TGG	-	chr3.3:53943256-53943275	MS.gene008574:intron	35.0%
!!	GGTTCTTTTGGTTGAAGTAA+TGG	+	chr3.3:53942807-53942826	None:intergenic	35.0%
!!	GTTACAACTTTTGAGTCACT+TGG	-	chr3.3:53942954-53942973	MS.gene008574:CDS	35.0%
!!	TCTTTTGGTTGAAGTAATGG+TGG	+	chr3.3:53942804-53942823	None:intergenic	35.0%
!!!	TTGTTTTAAGGTTGAGAGTG+AGG	-	chr3.3:53942875-53942894	MS.gene008574:CDS	35.0%
	AAAGACGCAAACATGTTGCA+TGG	+	chr3.3:53943389-53943408	None:intergenic	40.0%
	AACATGTTTGCGTCTTTGGA+AGG	-	chr3.3:53943392-53943411	MS.gene008574:CDS	40.0%
	ATGCAACATGTTTGCGTCTT+TGG	-	chr3.3:53943388-53943407	MS.gene008574:CDS	40.0%
	CCATAATACCCTATAGGCAA+AGG	+	chr3.3:53943086-53943105	None:intergenic	40.0%
	GATGATCCATCAATGAAAGG+AGG	-	chr3.3:53943452-53943471	MS.gene008574:CDS	40.0%
	GCCTTACAACTTATCACCAA+TGG	-	chr3.3:53943005-53943024	MS.gene008574:CDS	40.0%
	GGTGGTTTCACAATTGGAAT+GGG	-	chr3.3:53942627-53942646	MS.gene008574:CDS	40.0%
	GGTTATGATCCTTTGCCTAT+AGG	-	chr3.3:53943074-53943093	MS.gene008574:CDS	40.0%
	GTCAAGCTGTTCATGTATAC+CGG	-	chr3.3:53942110-53942129	MS.gene008574:CDS	40.0%
	GTGGTTTCACAATTGGAATG+GGG	-	chr3.3:53942628-53942647	MS.gene008574:CDS	40.0%
	TGGTGGTTTCACAATTGGAA+TGG	-	chr3.3:53942626-53942645	MS.gene008574:CDS	40.0%
!	ACATGTTTGCGTCTTTGGAA+GGG	-	chr3.3:53943393-53943412	MS.gene008574:CDS	40.0%
!	CCTTTGCCTATAGGGTATTA+TGG	-	chr3.3:53943083-53943102	MS.gene008574:CDS	40.0%
!	CGAGTTTTTGACGTAATCAC+TGG	+	chr3.3:53943216-53943235	None:intergenic	40.0%
!	GGATTGAGAAGTTGGTTTAG+TGG	+	chr3.3:53942371-53942390	None:intergenic	40.0%
!	GTTTTTGACGTAATCACTGG+TGG	+	chr3.3:53943213-53943232	None:intergenic	40.0%
	AACTTCTCAATCCGTTTCCC+AGG	-	chr3.3:53942378-53942397	MS.gene008574:CDS	45.0%
	CAAAAACTCGATCGACACTC+CGG	-	chr3.3:53943226-53943245	MS.gene008574:intron	45.0%
	GGAAGACTTAGAGTCGTGTT+TGG	-	chr3.3:53942426-53942445	MS.gene008574:CDS	45.0%
	GGTATTAGACGCAGCATGAA+AGG	-	chr3.3:53943053-53943072	MS.gene008574:CDS	45.0%
	TGGGAAAGAGAGAGACTAGT+TGG	-	chr3.3:53942750-53942769	MS.gene008574:CDS	45.0%
	TTCCTCCTTGAACTTTGGCA+TGG	+	chr3.3:53943510-53943529	None:intergenic	45.0%
!	CAAGAAGGAATGGACTTGGA+TGG	+	chr3.3:53942040-53942059	None:intergenic	45.0%
!	CATGTCTCACTAGTCTACCT+GGG	+	chr3.3:53942398-53942417	None:intergenic	45.0%
!	CTTAGAGTCGTGTTTGGAGT+TGG	-	chr3.3:53942432-53942451	MS.gene008574:CDS	45.0%
!	GGTTGAAGTAATGGTGGAAG+TGG	+	chr3.3:53942798-53942817	None:intergenic	45.0%
!	TCACTAGTCTACCTGGGAAA+CGG	+	chr3.3:53942392-53942411	None:intergenic	45.0%
!!	ACTTGGTGCTTATGTGTGGA+GGG	-	chr3.3:53942971-53942990	MS.gene008574:CDS	45.0%
!!	AGAAGCACTCTCAAAGGCTT+TGG	-	chr3.3:53942186-53942205	MS.gene008574:CDS	45.0%
!!	AGTCACTTGGTGCTTATGTG+TGG	-	chr3.3:53942967-53942986	MS.gene008574:CDS	45.0%
!!	CTGACTGAGTGGAAGCATTT+AGG	-	chr3.3:53943302-53943321	MS.gene008574:CDS	45.0%
!!	GATTTTAGCATGGACGGTAG+CGG	-	chr3.3:53943266-53943285	MS.gene008574:intron	45.0%
!!	TGGGAAACGGATTGAGAAGT+TGG	+	chr3.3:53942379-53942398	None:intergenic	45.0%
!!	TAAATAAATAAATAAACAAA+CGG	+	chr3.3:53942316-53942335	None:intergenic	5.0%
	AACTTTGGCATGGCAGCAAC+AGG	+	chr3.3:53943500-53943519	None:intergenic	50.0%
	ACTTTGGCATGGCAGCAACA+GGG	+	chr3.3:53943499-53943518	None:intergenic	50.0%
	AGCCTTCTGTGTTACCTGTG+TGG	-	chr3.3:53942730-53942749	MS.gene008574:CDS	50.0%
	AGTCTCTCTCTTTCCCACAC+AGG	+	chr3.3:53942747-53942766	None:intergenic	50.0%
	CGGTAACGATTCTGACTGAG+TGG	-	chr3.3:53943291-53943310	MS.gene008574:intron	50.0%
	GACTCTAAGTCTTCCGTCTC+CGG	+	chr3.3:53942421-53942440	None:intergenic	50.0%
	GCAAACATGTTGCATGGTGC+TGG	+	chr3.3:53943383-53943402	None:intergenic	50.0%
	GCCTTCTGTGTTACCTGTGT+GGG	-	chr3.3:53942731-53942750	MS.gene008574:CDS	50.0%
	GGTAGACTAGTGAGACATGC+CGG	-	chr3.3:53942399-53942418	MS.gene008574:CDS	50.0%
	TCCCACACAGGTAACACAGA+AGG	+	chr3.3:53942735-53942754	None:intergenic	50.0%
	TCTTGCTTATGCTCCGTCTC+CGG	+	chr3.3:53943248-53943267	None:intergenic	50.0%
	TGCCATGCCAAAGTTCAAGG+AGG	-	chr3.3:53943505-53943524	MS.gene008574:CDS	50.0%
	TGCTGCCATGCCAAAGTTCA+AGG	-	chr3.3:53943502-53943521	MS.gene008574:CDS	50.0%
	TGGTGGAAGTGGTTGTTTGC+TGG	+	chr3.3:53942787-53942806	None:intergenic	50.0%
!	AGAGTCGTGTTTGGAGTTGG+TGG	-	chr3.3:53942435-53942454	MS.gene008574:CDS	50.0%
!	GCATGTCTCACTAGTCTACC+TGG	+	chr3.3:53942399-53942418	None:intergenic	50.0%
!!	CACTTGGTGCTTATGTGTGG+AGG	-	chr3.3:53942970-53942989	MS.gene008574:CDS	50.0%
!!	GGAGTTGGTGGTGCAGAATA+TGG	-	chr3.3:53942447-53942466	MS.gene008574:CDS	50.0%
	CTAGTGAGACATGCCGGAGA+CGG	-	chr3.3:53942405-53942424	MS.gene008574:CDS	55.0%
	CTCGATCGACACTCCGGAGA+CGG	-	chr3.3:53943232-53943251	MS.gene008574:intron	60.0%
!	TAGCATGGACGGTAGCGGTG+CGG	-	chr3.3:53943271-53943290	MS.gene008574:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	53941974	53943556	53941974	ID=MS.gene008574
chr3.3	mRNA	53941974	53943556	53941974	ID=MS.gene008574.t1;Parent=MS.gene008574
chr3.3	exon	53943301	53943556	53943301	ID=MS.gene008574.t1.exon1;Parent=MS.gene008574.t1
chr3.3	CDS	53943301	53943556	53943301	ID=cds.MS.gene008574.t1;Parent=MS.gene008574.t1
chr3.3	exon	53941974	53943130	53941974	ID=MS.gene008574.t1.exon2;Parent=MS.gene008574.t1
chr3.3	CDS	53941974	53943130	53941974	ID=cds.MS.gene008574.t1;Parent=MS.gene008574.t1

Gene Sequence

>MS.gene008574

ATGGCGTATCAGAAAGCACCCCTAAACCTAAAGCTAGAAATGAAAGATGTTGTGCTTGTAAAACCATCCAAGTCCATTCCTTCTTGTATTCTTTCTCTGTCTACACTTGACAATAAAGACAACAATAATAACCTTTGTCAAGCTGTTCATGTATACCGGCCATCAACAATTCATGATTCTGATTCGGGTTTCGACCCTTGTCATGTATTCAAAGAAGCACTCTCAAAGGCTTTGGATTATTATTATCCTCTTGCAGGTATACTTGTAAAAAAGAACTTTTATGATGATAAATGTTACTAATGAATTGTTATAGCATAAATTTATGTGTGTATCCAAACACCGTTTGTTTATTTATTTATTTATGCTTGTGACCATAGAAGAATGTCTCGTTAAACCACTAAACCAACTTCTCAATCCGTTTCCCAGGTAGACTAGTGAGACATGCCGGAGACGGAAGACTTAGAGTCGTGTTTGGAGTTGGTGGTGCAGAATATGGTGTTCCATTCTTAGAAGCATATGCTAATTGCACCCTTTCTTCTCTTCATTACTTGGACAATACTGACACAGATATTGCAAAAGACTTGGTGCTTGATGTTCCTTCTCCTCAAGATAAAAGTTACCCTTTGCTATTCATGGTGACAAAATTTATTTGTGGTGGTTTCACAATTGGAATGGGGTGTTCACATGTTGTTTGTGATGGATATGGTTCAATTCAAGTCTTCAAAGCAATTGTTGAACTTGCAAAAGGAAGAAGTGAGCCTTCTGTGTTACCTGTGTGGGAAAGAGAGAGACTAGTTGGTTCAACAAGTACCAGCAAACAACCACTTCCACCATTACTTCAACCAAAAGAACCTGTTGCATTTTCACCTTTAGTTCAACCAAAAACAGTTATTAAAATCTATTGTTTTAAGGTTGAGAGTGAGGTGATAAAAAAACTTAAGATGAGTTTGATGAAGGAAGGTGATAGTGAAAGTGAACCAGTTACAACTTTTGAGTCACTTGGTGCTTATGTGTGGAGGGCAAGAGCAAGAGCCTTACAACTTATCACCAATGGAAAAGCAATGTTGAATATGTTGGTTGGTATTAGACGCAGCATGAAAGGTTATGATCCTTTGCCTATAGGGTATTATGGTAATACTACTGTGGATGGAAAAGTTGTTTTAAAAGTGAGTGAGCTCAATGAAATGCCACTCTATGAAATTGTTAAGTTGATCAAAGAGACTAAAAAGGTTGCTTCCACCAGTGATTACGTCAAAAACTCGATCGACACTCCGGAGACGGAGCATAAGCAAGATTTTAGCATGGACGGTAGCGGTGCGGTAACGATTCTGACTGAGTGGAAGCATTTAGGTTTTCTTGATGAAAATAGTATAGATTTTGGAGGGTATGAATCAGTGAATTTTGTACCAGCACCATGCAACATGTTTGCGTCTTTGGAAGGGTGCATTTTCGCATCTCCTAGTAAAGTTGATGATGATGATGATCCATCAATGAAAGGAGGAGTTAAGATTTTCACATCACTCCCTGTTGCTGCCATGCCAAAGTTCAAGGAGGAAATTGAAGCTTTAAGATTACTTAATTGA

Protein sequence

>MS.gene008574.t1

MAYQKAPLNLKLEMKDVVLVKPSKSIPSCILSLSTLDNKDNNNNLCQAVHVYRPSTIHDSDSGFDPCHVFKEALSKALDYYYPLAGRLVRHAGDGRLRVVFGVGGAEYGVPFLEAYANCTLSSLHYLDNTDTDIAKDLVLDVPSPQDKSYPLLFMVTKFICGGFTIGMGCSHVVCDGYGSIQVFKAIVELAKGRSEPSVLPVWERERLVGSTSTSKQPLPPLLQPKEPVAFSPLVQPKTVIKIYCFKVESEVIKKLKMSLMKEGDSESEPVTTFESLGAYVWRARARALQLITNGKAMLNMLVGIRRSMKGYDPLPIGYYGNTTVDGKVVLKVSELNEMPLYEIVKLIKETKKVASTSDYVKNSIDTPETEHKQDFSMDGSGAVTILTEWKHLGFLDENSIDFGGYESVNFVPAPCNMFASLEGCIFASPSKVDDDDDPSMKGGVKIFTSLPVAAMPKFKEEIEALRLLN