AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49765


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49765.t1 MTR_8g096080 92.342 222 15 1 1 220 1 222 2.13e-153 424
MS.gene49765.t1 MTR_8g096110 82.648 219 38 0 1 219 1 219 8.34e-129 362
MS.gene49765.t1 MTR_8g096100 72.642 212 57 1 1 211 1 212 1.56e-106 306
MS.gene49765.t1 MTR_3g098490 66.842 190 56 3 1 187 1 186 3.03e-90 264
MS.gene49765.t1 MTR_3g098500 61.579 190 70 2 1 187 7 196 8.12e-83 246
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49765.t1 AT3G23760 62.162 185 67 2 9 191 11 194 1.41e-83 247
MS.gene49765.t1 AT4G14100 55.556 198 87 1 9 206 8 204 6.34e-81 240

Find 58 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCCTTCTGTTGTGTATT+TGG 0.136397 4.4:+7285449 None:intergenic
GCTTGATGGTGCTAATTATT+TGG 0.169784 4.4:-7285790 MS.gene49765:CDS
GCTCCTTCTGTTGTGTATTT+GGG 0.213711 4.4:+7285450 None:intergenic
TCCGGCCACGGCGCCGGAAT+TGG 0.215529 4.4:+7286041 None:intergenic
GGGAGAAAGTCGAATTTATT+TGG 0.267906 4.4:-7285731 MS.gene49765:CDS
CCACGACCCAATTGGCTTGA+TGG 0.304709 4.4:-7285804 MS.gene49765:CDS
CTTGATGGTGCTAATTATTT+GGG 0.312059 4.4:-7285789 MS.gene49765:CDS
CCGGCCACGGCGCCGGAATT+GGG 0.367218 4.4:+7286042 None:intergenic
TGAGAACATCAAGCTGGTAT+TGG 0.386461 4.4:+7285922 None:intergenic
AGGACTGAGTGGAACTGTTC+CGG 0.404511 4.4:+7286023 None:intergenic
ATTAGCACCATCAAGCCAAT+TGG 0.405118 4.4:+7285797 None:intergenic
GACCACAGAGGACCCAATTC+CGG 0.405830 4.4:-7286054 MS.gene49765:CDS
TTCTCAAATATGACCTTGAA+TGG 0.424620 4.4:-7285905 MS.gene49765:CDS
AATGTTGAAATTTCGTCCAT+TGG 0.430457 4.4:+7285947 None:intergenic
ATTTGAGGTTGGAGCAGTAC+TGG 0.443095 4.4:-7285527 MS.gene49765:CDS
ATGTTGAAATTTCGTCCATT+GGG 0.444878 4.4:+7285948 None:intergenic
CTCTTCGAAAACAAGACTTA+TGG 0.444999 4.4:-7285980 MS.gene49765:CDS
AACTGTTCCGGCCACGGCGC+CGG 0.448851 4.4:+7286035 None:intergenic
TGGTGCCATTGTTCCATTCA+AGG 0.450465 4.4:+7285892 None:intergenic
CCCAATTCCGGCGCCGTGGC+CGG 0.459468 4.4:-7286042 MS.gene49765:CDS
TTGGTATTCCACGACCCAAT+TGG 0.459870 4.4:-7285812 MS.gene49765:CDS
AACATTACACAGAAAATTGT+CGG 0.472163 4.4:+7285755 None:intergenic
ACTAATGAGTGAGATGAGAT+AGG 0.485469 4.4:-7285377 None:intergenic
CTCCTTCTGTTGTGTATTTG+GGG 0.486624 4.4:+7285451 None:intergenic
ACTGGATGATGCCCATTGGC+AGG 0.511220 4.4:-7285509 MS.gene49765:CDS
AGCCGCTTCTGTTGAGGAGT+AGG 0.512649 4.4:+7286003 None:intergenic
ACTTTGCAAGTGTGCTCGAA+AGG 0.514114 4.4:+7285849 None:intergenic
TTAGCACCATCAAGCCAATT+GGG 0.517591 4.4:+7285798 None:intergenic
AACAAGACTTATGGTACGAT+TGG 0.522229 4.4:-7285971 MS.gene49765:CDS
GAGTGGAACTGTTCCGGCCA+CGG 0.525676 4.4:+7286029 None:intergenic
TGGTCGAAGTTGAGAGATGA+AGG 0.525737 4.4:+7286072 None:intergenic
GAAGAGAGCCGCTTCTGTTG+AGG 0.544198 4.4:+7285997 None:intergenic
TACTGATGTGATGACATTTG+AGG 0.549841 4.4:-7285542 MS.gene49765:CDS
AATGCATTCTTCTGTGGCAG+AGG 0.552048 4.4:+7286121 None:intergenic
AATAGACAGGAGCCTGCCAA+TGG 0.552630 4.4:+7285497 None:intergenic
TTATGGTACGATTGGCCCAA+TGG 0.556501 4.4:-7285963 MS.gene49765:CDS
CTTTGCAAGTGTGCTCGAAA+GGG 0.564893 4.4:+7285850 None:intergenic
AAGTTGAGAGATGAAGGAAG+AGG 0.569210 4.4:+7286078 None:intergenic
CCATCAAGCCAATTGGGTCG+TGG 0.576651 4.4:+7285804 None:intergenic
ATAGACAGGAGCCTGCCAAT+GGG 0.577912 4.4:+7285498 None:intergenic
GTCCTACTCCTCAACAGAAG+CGG 0.579821 4.4:-7286005 MS.gene49765:CDS
AACCCCAAATACACAACAGA+AGG 0.582352 4.4:-7285453 MS.gene49765:CDS
TCGTAGAAGATCCACTTGAC+AGG 0.590706 4.4:+7285681 None:intergenic
TCACTAAAAGACCTGTCAAG+TGG 0.590795 4.4:-7285692 MS.gene49765:CDS
TATGACCTTGAATGGAACAA+TGG 0.591932 4.4:-7285897 MS.gene49765:CDS
GCCTTACTAAAACAATAGAC+AGG 0.594404 4.4:+7285484 None:intergenic
CATATTTGAGAACATCAAGC+TGG 0.600302 4.4:+7285916 None:intergenic
AGGGTCGAGAGTGTAGAGAA+AGG 0.604953 4.4:+7285869 None:intergenic
CAAACAAATGCATTCTTCTG+TGG 0.604964 4.4:+7286115 None:intergenic
CAGTACTGGATGATGCCCAT+TGG 0.615897 4.4:-7285513 MS.gene49765:CDS
CGCCGGAATTGGGTCCTCTG+TGG 0.626171 4.4:+7286052 None:intergenic
GATGTGATGACATTTGAGGT+TGG 0.629445 4.4:-7285538 MS.gene49765:CDS
GTCAAGTGGATCTTCTACGA+TGG 0.629602 4.4:-7285678 MS.gene49765:intron
AGGACCCAATTCCGGCGCCG+TGG 0.635409 4.4:-7286046 MS.gene49765:CDS
AAAGTGACTCAATATGATGT+TGG 0.647948 4.4:-7285831 MS.gene49765:CDS
TGTTGAGGAGTAGGACTGAG+TGG 0.648618 4.4:+7286012 None:intergenic
GTCGAGAGTGTAGAGAAAGG+TGG 0.677328 4.4:+7285872 None:intergenic
TCTCTCAACTTCGACCACAG+AGG 0.720290 4.4:-7286066 MS.gene49765:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATTCATTATATGATGAT+TGG - chr4.4:7285922-7285941 MS.gene49765:CDS 15.0%
! AACATTACACAGAAAATTGT+CGG + chr4.4:7285751-7285770 None:intergenic 25.0%
! CTACATATAGCTAAAATTAC+TGG - chr4.4:7285876-7285895 MS.gene49765:CDS 25.0%
!! ACAATTTTCTGTGTAATGTT+TGG - chr4.4:7285751-7285770 MS.gene49765:CDS 25.0%
!! CAATTTTCTGTGTAATGTTT+GGG - chr4.4:7285752-7285771 MS.gene49765:CDS 25.0%
AAAGTGACTCAATATGATGT+TGG - chr4.4:7285672-7285691 MS.gene49765:intron 30.0%
AATGTTGAAATTTCGTCCAT+TGG + chr4.4:7285559-7285578 None:intergenic 30.0%
ATGTTGAAATTTCGTCCATT+GGG + chr4.4:7285558-7285577 None:intergenic 30.0%
TTCTCAAATATGACCTTGAA+TGG - chr4.4:7285598-7285617 MS.gene49765:intron 30.0%
!! CTTGATGGTGCTAATTATTT+GGG - chr4.4:7285714-7285733 MS.gene49765:CDS 30.0%
!!! CTATTGTTGATGATTTTCGT+TGG - chr4.4:7286101-7286120 MS.gene49765:CDS 30.0%
!!! TCCTGTCTATTGTTTTAGTA+AGG - chr4.4:7286018-7286037 MS.gene49765:CDS 30.0%
AACAAGACTTATGGTACGAT+TGG - chr4.4:7285532-7285551 MS.gene49765:CDS 35.0%
CAAACAAATGCATTCTTCTG+TGG + chr4.4:7285391-7285410 None:intergenic 35.0%
CATATTTGAGAACATCAAGC+TGG + chr4.4:7285590-7285609 None:intergenic 35.0%
CTCTTCGAAAACAAGACTTA+TGG - chr4.4:7285523-7285542 MS.gene49765:CDS 35.0%
GCCTTACTAAAACAATAGAC+AGG + chr4.4:7286022-7286041 None:intergenic 35.0%
GGGAGAAAGTCGAATTTATT+TGG - chr4.4:7285772-7285791 MS.gene49765:CDS 35.0%
TACTGATGTGATGACATTTG+AGG - chr4.4:7285961-7285980 MS.gene49765:CDS 35.0%
TATGACCTTGAATGGAACAA+TGG - chr4.4:7285606-7285625 MS.gene49765:intron 35.0%
!! GCTTGATGGTGCTAATTATT+TGG - chr4.4:7285713-7285732 MS.gene49765:CDS 35.0%
!!! CTATTGTTTTAGTAAGGCTG+AGG - chr4.4:7286024-7286043 MS.gene49765:CDS 35.0%
AACCCCAAATACACAACAGA+AGG - chr4.4:7286050-7286069 MS.gene49765:CDS 40.0%
AAGTTGAGAGATGAAGGAAG+AGG + chr4.4:7285428-7285447 None:intergenic 40.0%
ATATGATGATTGGTGAGTGC+AGG - chr4.4:7285932-7285951 MS.gene49765:CDS 40.0%
ATTAGCACCATCAAGCCAAT+TGG + chr4.4:7285709-7285728 None:intergenic 40.0%
CTCCTTCTGTTGTGTATTTG+GGG + chr4.4:7286055-7286074 None:intergenic 40.0%
GCTCCTTCTGTTGTGTATTT+GGG + chr4.4:7286056-7286075 None:intergenic 40.0%
TCACTAAAAGACCTGTCAAG+TGG - chr4.4:7285811-7285830 MS.gene49765:CDS 40.0%
TGAGAACATCAAGCTGGTAT+TGG + chr4.4:7285584-7285603 None:intergenic 40.0%
TGCTCCTTCTGTTGTGTATT+TGG + chr4.4:7286057-7286076 None:intergenic 40.0%
TTAGCACCATCAAGCCAATT+GGG + chr4.4:7285708-7285727 None:intergenic 40.0%
! GATGTGATGACATTTGAGGT+TGG - chr4.4:7285965-7285984 MS.gene49765:CDS 40.0%
AATGCATTCTTCTGTGGCAG+AGG + chr4.4:7285385-7285404 None:intergenic 45.0%
ACTTTGCAAGTGTGCTCGAA+AGG + chr4.4:7285657-7285676 None:intergenic 45.0%
CTTTGCAAGTGTGCTCGAAA+GGG + chr4.4:7285656-7285675 None:intergenic 45.0%
GTCAAGTGGATCTTCTACGA+TGG - chr4.4:7285825-7285844 MS.gene49765:CDS 45.0%
TCGTAGAAGATCCACTTGAC+AGG + chr4.4:7285825-7285844 None:intergenic 45.0%
TGGTCGAAGTTGAGAGATGA+AGG + chr4.4:7285434-7285453 None:intergenic 45.0%
TTGGTATTCCACGACCCAAT+TGG - chr4.4:7285691-7285710 MS.gene49765:CDS 45.0%
! ATTTGAGGTTGGAGCAGTAC+TGG - chr4.4:7285976-7285995 MS.gene49765:CDS 45.0%
! TTATGGTACGATTGGCCCAA+TGG - chr4.4:7285540-7285559 MS.gene49765:CDS 45.0%
!! TGGTGCCATTGTTCCATTCA+AGG + chr4.4:7285614-7285633 None:intergenic 45.0%
AATAGACAGGAGCCTGCCAA+TGG + chr4.4:7286009-7286028 None:intergenic 50.0%
AGGACTGAGTGGAACTGTTC+CGG + chr4.4:7285483-7285502 None:intergenic 50.0%
ATAGACAGGAGCCTGCCAAT+GGG + chr4.4:7286008-7286027 None:intergenic 50.0%
GTCCTACTCCTCAACAGAAG+CGG - chr4.4:7285498-7285517 MS.gene49765:CDS 50.0%
TCTCTCAACTTCGACCACAG+AGG - chr4.4:7285437-7285456 MS.gene49765:CDS 50.0%
TGTTGAGGAGTAGGACTGAG+TGG + chr4.4:7285494-7285513 None:intergenic 50.0%
! AGGGTCGAGAGTGTAGAGAA+AGG + chr4.4:7285637-7285656 None:intergenic 50.0%
! CAGTACTGGATGATGCCCAT+TGG - chr4.4:7285990-7286009 MS.gene49765:CDS 50.0%
! GTCGAGAGTGTAGAGAAAGG+TGG + chr4.4:7285634-7285653 None:intergenic 50.0%
ACTGGATGATGCCCATTGGC+AGG - chr4.4:7285994-7286013 MS.gene49765:CDS 55.0%
AGCCGCTTCTGTTGAGGAGT+AGG + chr4.4:7285503-7285522 None:intergenic 55.0%
CCATCAAGCCAATTGGGTCG+TGG + chr4.4:7285702-7285721 None:intergenic 55.0%
GAAGAGAGCCGCTTCTGTTG+AGG + chr4.4:7285509-7285528 None:intergenic 55.0%
GACCACAGAGGACCCAATTC+CGG - chr4.4:7285449-7285468 MS.gene49765:CDS 55.0%
!! CCACGACCCAATTGGCTTGA+TGG - chr4.4:7285699-7285718 MS.gene49765:CDS 55.0%
GAGTGGAACTGTTCCGGCCA+CGG + chr4.4:7285477-7285496 None:intergenic 60.0%
CGCCGGAATTGGGTCCTCTG+TGG + chr4.4:7285454-7285473 None:intergenic 65.0%
AACTGTTCCGGCCACGGCGC+CGG + chr4.4:7285471-7285490 None:intergenic 70.0%
AGGACCCAATTCCGGCGCCG+TGG - chr4.4:7285457-7285476 MS.gene49765:CDS 70.0%
CCCAATTCCGGCGCCGTGGC+CGG - chr4.4:7285461-7285480 MS.gene49765:CDS 75.0%
! CCGGCCACGGCGCCGGAATT+GGG + chr4.4:7285464-7285483 None:intergenic 75.0%
! TCCGGCCACGGCGCCGGAAT+TGG + chr4.4:7285465-7285484 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 7285378 7286147 7285378 ID=MS.gene49765
chr4.4 mRNA 7285378 7286147 7285378 ID=MS.gene49765.t1;Parent=MS.gene49765
chr4.4 exon 7285679 7286147 7285679 ID=MS.gene49765.t1.exon1;Parent=MS.gene49765.t1
chr4.4 CDS 7285679 7286147 7285679 ID=cds.MS.gene49765.t1;Parent=MS.gene49765.t1
chr4.4 exon 7285378 7285571 7285378 ID=MS.gene49765.t1.exon2;Parent=MS.gene49765.t1
chr4.4 CDS 7285378 7285571 7285378 ID=cds.MS.gene49765.t1;Parent=MS.gene49765.t1
Gene Sequence

>MS.gene49765

ATGGCCTCTGCCACAGAAGAATGCATTTGTTTGTTTTTGTGTCTCTTCCTCTTCCTTCATCTCTCAACTTCGACCACAGAGGACCCAATTCCGGCGCCGTGGCCGGAACAGTTCCACTCAGTCCTACTCCTCAACAGAAGCGGCTCTCTTCGAAAACAAGACTTATGGTACGATTGGCCCAATGGACGAAATTTCAACATTATCCAATACCAGCTTGATGTTCTCAAATATGACCTTGAATGGAACAATGGCACCACCTTTCTCTACACTCTCGACCCTTTCGAGCACACTTGCAAAGTGACTCAATATGATGTTGGTATTCCACGACCCAATTGGCTTGATGGTGCTAATTATTTGGGTCAACAATATGCCGACAATTTTCTGTGTAATGTTTGGGAGAAAGTCGAATTTATTTGGTACTTTGAAGATGTTCTCACTAAAAGACCTGTCAAGTGGATCTTCTACGATGGTTAGTTGCTTTAATTTGAAGTTTTCGTGCTACATATAGCTAAAATTACTGGCTAGAAAAATATGTTATGTTTATTAAATTCATTATATGATGATTGGTGAGTGCAGGAATGATTACTGATGTGATGACATTTGAGGTTGGAGCAGTACTGGATGATGCCCATTGGCAGGCTCCTGTCTATTGTTTTAGTAAGGCTGAGGCTGAACCCCAAATACACAACAGAAGGAGCAGTTTCTCATTAGATTTAGAAGCTGCTATTGTTGATGATTTTCGTTGGACACTAATGAGTGAGATGAGATAG

Protein sequence

>MS.gene49765.t1

MASATEECICLFLCLFLFLHLSTSTTEDPIPAPWPEQFHSVLLLNRSGSLRKQDLWYDWPNGRNFNIIQYQLDVLKYDLEWNNGTTFLYTLDPFEHTCKVTQYDVGIPRPNWLDGANYLGQQYADNFLCNVWEKVEFIWYFEDVLTKRPVKWIFYDGMITDVMTFEVGAVLDDAHWQAPVYCFSKAEAEPQIHNRRSSFSLDLEAAIVDDFRWTLMSEMR