AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92366


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92366.t1 MTR_5g005200 88.811 143 6 1 1 133 1 143 4.24e-78 227
MS.gene92366.t1 MTR_5g025500 42.105 95 55 0 38 132 37 131 1.58e-24 91.7
MS.gene92366.t1 MTR_3g053350 51.327 113 11 5 21 129 29 101 1.16e-18 81.3
MS.gene92366.t1 MTR_3g055330 41.111 90 51 2 44 132 147 235 1.33e-15 71.6
MS.gene92366.t1 MTR_3g055330 41.111 90 51 2 44 132 147 235 2.04e-15 71.2
MS.gene92366.t1 MTR_5g018010 40.580 69 40 1 62 130 61 128 4.10e-15 69.7
MS.gene92366.t1 MTR_5g018010 37.805 82 50 1 51 132 183 263 2.13e-14 67.8
MS.gene92366.t1 MTR_5g018010 40.580 69 40 1 62 130 138 205 1.18e-14 69.3
MS.gene92366.t1 MTR_5g018010 37.805 82 50 1 51 132 260 340 5.25e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92366.t1 AT2G14880 55.944 143 50 6 1 132 1 141 4.37e-42 136
MS.gene92366.t1 AT4G34290 56.034 116 39 2 29 132 29 144 1.03e-34 117
MS.gene92366.t1 AT3G03590 52.564 78 37 0 55 132 63 140 2.18e-25 94.0
MS.gene92366.t1 AT1G31760 40.594 101 59 1 32 131 8 108 6.58e-25 92.0
MS.gene92366.t1 AT2G35605 47.253 91 48 0 41 131 15 105 1.22e-24 91.3
MS.gene92366.t1 AT2G14880 56.471 85 32 4 13 94 21 103 3.94e-21 82.0
MS.gene92366.t1 AT2G14880 56.471 85 32 4 13 94 21 103 3.94e-21 82.0
MS.gene92366.t1 AT4G26810 34.000 100 61 2 32 131 1 95 1.57e-15 67.8
MS.gene92366.t1 AT4G26810 34.000 100 61 2 32 131 1 95 1.57e-15 67.8
MS.gene92366.t1 AT4G22360 37.624 101 54 3 40 132 173 272 1.90e-15 71.6
MS.gene92366.t1 AT1G49520 37.209 86 50 2 45 130 243 324 1.87e-13 66.2
MS.gene92366.t1 AT1G49520 32.895 76 50 1 55 130 101 175 1.99e-11 60.5
MS.gene92366.t1 AT3G48600 33.019 106 67 2 32 133 8 113 6.20e-13 62.8
MS.gene92366.t1 AT3G19080 35.165 91 56 2 40 130 250 337 8.30e-13 64.3
MS.gene92366.t1 AT3G19080 34.211 76 49 1 55 130 115 189 1.26e-11 60.8
MS.gene92366.t1 AT3G48600 33.019 106 67 2 32 133 8 113 1.41e-12 62.8
MS.gene92366.t1 AT3G19080 34.783 92 57 2 40 131 250 338 1.52e-12 63.5
MS.gene92366.t1 AT3G19080 33.766 77 50 1 55 131 115 190 1.93e-11 60.5
MS.gene92366.t1 AT3G19080 34.783 92 57 2 40 131 250 338 1.52e-12 63.5
MS.gene92366.t1 AT3G19080 33.766 77 50 1 55 131 115 190 1.93e-11 60.5
MS.gene92366.t1 AT3G19080 34.783 92 57 2 40 131 250 338 1.56e-12 63.5
MS.gene92366.t1 AT3G19080 33.766 77 50 1 55 131 115 190 2.03e-11 60.5

Find 48 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCAAATGCTCTTGAGGGCT+TGG 0.201712 5.1:-252722 None:intergenic
GTGGCCTTTGCCTTCAATTA+AGG 0.272510 5.1:-253744 None:intergenic
GCTCTTGAGGGCTTGGGTTC+GGG 0.285856 5.1:-252715 None:intergenic
TGCTCTTGAGGGCTTGGGTT+CGG 0.309630 5.1:-252716 None:intergenic
CCCAAGCCCTCAAGAGCATT+TGG 0.313567 5.1:+252721 MS.gene92366:CDS
GAGCATTTGGGCCCACATTA+AGG 0.313899 5.1:+252734 MS.gene92366:CDS
CTTGGAGTCCTGAGAGTTAG+AGG 0.343205 5.1:-252531 None:intergenic
CCAAATGCTCTTGAGGGCTT+GGG 0.352861 5.1:-252721 None:intergenic
GGCTTGGGTTCGGGAAATCT+CGG 0.358689 5.1:-252706 None:intergenic
GTTGATATGCTTGAGATTGC+TGG 0.364505 5.1:+253703 MS.gene92366:CDS
GCCCTCACTTCCTTAATTGA+AGG 0.384410 5.1:+253734 MS.gene92366:CDS
CCGACGAGGTCTTGCATCTC+GGG 0.390790 5.1:-252678 None:intergenic
GCCGACGAGGTCTTGCATCT+CGG 0.410466 5.1:-252679 None:intergenic
AAACTGAAGAAGGTATTTGC+AGG 0.431242 5.1:+253670 MS.gene92366:CDS
CCAAGCCCTCAAGAGCATTT+GGG 0.438538 5.1:+252722 MS.gene92366:CDS
GCTTGGGTTCGGGAAATCTC+GGG 0.463473 5.1:-252705 None:intergenic
TCCCACACCTTCGTTGCGTA+TGG 0.464306 5.1:+252563 MS.gene92366:CDS
CATCTCCTCCTCTAACTCTC+AGG 0.478833 5.1:+252523 MS.gene92366:CDS
CATTAAGGAACACAATCTTC+AGG 0.486338 5.1:+252749 MS.gene92366:CDS
GTCTCTCAGCCTCCACCCAC+CGG 0.498374 5.1:+252615 MS.gene92366:CDS
GCCTTCAATTAAGGAAGTGA+GGG 0.502350 5.1:-253735 None:intergenic
GTGGGCCCAAATGCTCTTGA+GGG 0.503891 5.1:-252727 None:intergenic
CTCTAACTCTCAGGACTCCA+AGG 0.525205 5.1:+252532 MS.gene92366:CDS
TGTGGGCCCAAATGCTCTTG+AGG 0.532300 5.1:-252728 None:intergenic
GAAGATTGTGTTCCTTAATG+TGG 0.537334 5.1:-252746 None:intergenic
AGTGAAGATGGCGGTTTCAA+TGG 0.537531 5.1:+252482 None:intergenic
AGGAGATGATAATAATAGTC+CGG 0.546560 5.1:-252508 None:intergenic
GGTGGGTGGAGGCTGAGAGA+CGG 0.548280 5.1:-252613 None:intergenic
CGGTGGCAGAAGTAACTGTT+CGG 0.553786 5.1:-252593 None:intergenic
TGCCTTCAATTAAGGAAGTG+AGG 0.555494 5.1:-253736 None:intergenic
AAGATTGTGTTCCTTAATGT+GGG 0.565657 5.1:-252745 None:intergenic
AGCCATACGCAACGAAGGTG+TGG 0.571765 5.1:-252565 None:intergenic
GCCATACGCAACGAAGGTGT+GGG 0.575259 5.1:-252564 None:intergenic
CTGTGATGAGAAACTGAAGA+AGG 0.578111 5.1:+253660 MS.gene92366:CDS
ATGGCGGTTTCAATGGCTAC+CGG 0.582248 5.1:+252489 MS.gene92366:CDS
GGGTGGAGGCTGAGAGACGG+TGG 0.583952 5.1:-252610 None:intergenic
GGTGTGGGAAACGCAAACCT+TGG 0.584167 5.1:-252549 None:intergenic
AACTGAAGAAGGTATTTGCA+GGG 0.589332 5.1:+253671 MS.gene92366:CDS
CCCACCGGAGGCAAAATTCG+CGG 0.592965 5.1:+252630 MS.gene92366:CDS
AAATCTCGGGTTGGCCGACG+AGG 0.609292 5.1:-252692 None:intergenic
GGGTTCGGGAAATCTCGGGT+TGG 0.610231 5.1:-252701 None:intergenic
TCTCAGCCTCCACCCACCGG+AGG 0.623094 5.1:+252618 MS.gene92366:CDS
CGACGAGGTCTTGCATCTCG+GGG 0.623606 5.1:-252677 None:intergenic
CCCGAGATGCAAGACCTCGT+CGG 0.633591 5.1:+252678 MS.gene92366:CDS
GGAGTCCTGAGAGTTAGAGG+AGG 0.667796 5.1:-252528 None:intergenic
AGTGGAGCCATACGCAACGA+AGG 0.673850 5.1:-252570 None:intergenic
GACGAGGTCTTGCATCTCGG+GGG 0.684378 5.1:-252676 None:intergenic
GCAGAAGTAACTGTTCGGAG+TGG 0.693933 5.1:-252588 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAAAAACAAATAATTTGTA+CGG - chr5.1:252838-252857 None:intergenic 15.0%
!!! TTATTTTTAACCAACTAAAT+GGG + chr5.1:253113-253132 MS.gene92366:intron 15.0%
!!! TTTAGGTGAAATTTTAAAAA+AGG - chr5.1:252815-252834 None:intergenic 15.0%
!!! TTTATTTTTAACCAACTAAA+TGG + chr5.1:253112-253131 MS.gene92366:intron 15.0%
!! AACATGTAGTTTAGATTATA+TGG - chr5.1:253368-253387 None:intergenic 20.0%
!! ATATTATTAGGCACTAATTT+GGG + chr5.1:253020-253039 MS.gene92366:intron 20.0%
!! CAAAATAAAACATGAACAAA+TGG - chr5.1:252971-252990 None:intergenic 20.0%
!! GTCTGAATGAATATATTATT+AGG + chr5.1:253008-253027 MS.gene92366:intron 20.0%
!! TAAGTTGTTAGTTATTAAGA+TGG + chr5.1:252890-252909 MS.gene92366:intron 20.0%
!! TATATTATTAGGCACTAATT+TGG + chr5.1:253019-253038 MS.gene92366:intron 20.0%
!!! TATGCAATTCTGTTTTATAA+TGG + chr5.1:253308-253327 MS.gene92366:intron 20.0%
! AAAAGAATGAAAACGAGATT+TGG - chr5.1:253095-253114 None:intergenic 25.0%
! CATTCAATTTATGGATGATA+CGG - chr5.1:253509-253528 None:intergenic 25.0%
! TATATTCATTCAGACCAAAT+TGG - chr5.1:253003-253022 None:intergenic 25.0%
!! ACAAATAATTTGTACGGTTT+AGG - chr5.1:252832-252851 None:intergenic 25.0%
!! ATGGATTCTATTGTCAATTT+TGG + chr5.1:253340-253359 MS.gene92366:intron 25.0%
!!! CAAAAAGTTGATGTACAAAT+TGG - chr5.1:253142-253161 None:intergenic 25.0%
AAATTGGATTCCCATTTAGT+TGG - chr5.1:253126-253145 None:intergenic 30.0%
AAATTTGTGCAATTGAAACG+CGG - chr5.1:253273-253292 None:intergenic 30.0%
AAGATTGTGTTCCTTAATGT+GGG - chr5.1:252748-252767 None:intergenic 30.0%
AGGAGATGATAATAATAGTC+CGG - chr5.1:252511-252530 None:intergenic 30.0%
CCATAAATTGAATGTTCTCT+GGG + chr5.1:253515-253534 MS.gene92366:intron 30.0%
TAAACTGCATAGATTCAGAA+TGG + chr5.1:252867-252886 MS.gene92366:intron 30.0%
TCCATAAATTGAATGTTCTC+TGG + chr5.1:253514-253533 MS.gene92366:intron 30.0%
TGGGAATAATCGGTTATTAT+GGG + chr5.1:253039-253058 MS.gene92366:intron 30.0%
TTGGGAATAATCGGTTATTA+TGG + chr5.1:253038-253057 MS.gene92366:intron 30.0%
! TTTATAATGGTTGATGCTGA+TGG + chr5.1:253321-253340 MS.gene92366:intron 30.0%
!! TTTTGACTCAACAACCAATT+TGG + chr5.1:252986-253005 MS.gene92366:intron 30.0%
AAAGCAGAGTTAGTACTTGA+TGG - chr5.1:253249-253268 None:intergenic 35.0%
ACATAATTAACCTGTCTGTC+TGG + chr5.1:253223-253242 MS.gene92366:intron 35.0%
ATGTAGAACAAGTGCAAACA+AGG - chr5.1:253193-253212 None:intergenic 35.0%
CATTAAGGAACACAATCTTC+AGG + chr5.1:252749-252768 MS.gene92366:CDS 35.0%
CCCAGAGAACATTCAATTTA+TGG - chr5.1:253518-253537 None:intergenic 35.0%
CTGAACGTATTTATGAATGC+AGG - chr5.1:253567-253586 None:intergenic 35.0%
GAAGATTGTGTTCCTTAATG+TGG - chr5.1:252749-252768 None:intergenic 35.0%
GGAATAATCGGTTATTATGG+GGG + chr5.1:253041-253060 MS.gene92366:intron 35.0%
GGCACTAATTTGGGAATAAT+CGG + chr5.1:253029-253048 MS.gene92366:intron 35.0%
GGGAATAATCGGTTATTATG+GGG + chr5.1:253040-253059 MS.gene92366:intron 35.0%
TATCCTCAAGAGAAAGAGAA+AGG - chr5.1:252787-252806 None:intergenic 35.0%
TATGGGGGCTAAATTCAATT+GGG + chr5.1:253056-253075 MS.gene92366:intron 35.0%
TTATGAATGCAGGATAGCAT+TGG - chr5.1:253557-253576 None:intergenic 35.0%
TTATGGGGGCTAAATTCAAT+TGG + chr5.1:253055-253074 MS.gene92366:intron 35.0%
! GAATAAGCCTCTTTTTCTCA+GGG - chr5.1:253639-253658 None:intergenic 35.0%
!! AAACTGAAGAAGGTATTTGC+AGG + chr5.1:253670-253689 MS.gene92366:CDS 35.0%
!! AACTGAAGAAGGTATTTGCA+GGG + chr5.1:253671-253690 MS.gene92366:CDS 35.0%
CTGTGATGAGAAACTGAAGA+AGG + chr5.1:253660-253679 MS.gene92366:CDS 40.0%
GTCTGTACAAGAGTAGTTGT+AGG - chr5.1:253460-253479 None:intergenic 40.0%
GTTGATATGCTTGAGATTGC+TGG + chr5.1:253703-253722 MS.gene92366:CDS 40.0%
TACGTTCAGCAACGTTTGAT+TGG + chr5.1:253578-253597 MS.gene92366:intron 40.0%
TCTCCTTTCTCTTTCTCTTG+AGG + chr5.1:252781-252800 MS.gene92366:intron 40.0%
TTCAGAACCCTGAGAAAAAG+AGG + chr5.1:253629-253648 MS.gene92366:intron 40.0%
! CGAATAAGCCTCTTTTTCTC+AGG - chr5.1:253640-253659 None:intergenic 40.0%
! GCAGGATAGCATTGGATTAA+TGG - chr5.1:253549-253568 None:intergenic 40.0%
!!! ATCTCGGGGGAGATTTTTTT+AGG - chr5.1:252666-252685 None:intergenic 40.0%
! AATACCGCGAATTTTGCCTC+CGG - chr5.1:252637-252656 None:intergenic 45.0%
ATGGCGGTTTCAATGGCTAC+CGG + chr5.1:252489-252508 MS.gene92366:CDS 50.0%
CATCTCCTCCTCTAACTCTC+AGG + chr5.1:252523-252542 MS.gene92366:CDS 50.0%
CCAAATGCTCTTGAGGGCTT+GGG - chr5.1:252724-252743 None:intergenic 50.0%
CGGTGGCAGAAGTAACTGTT+CGG - chr5.1:252596-252615 None:intergenic 50.0%
CTCTAACTCTCAGGACTCCA+AGG + chr5.1:252532-252551 MS.gene92366:CDS 50.0%
CTTGGAGTCCTGAGAGTTAG+AGG - chr5.1:252534-252553 None:intergenic 50.0%
GAGCATTTGGGCCCACATTA+AGG + chr5.1:252734-252753 MS.gene92366:CDS 50.0%
GCAGAAGTAACTGTTCGGAG+TGG - chr5.1:252591-252610 None:intergenic 50.0%
! TACTTGATGGCCAGACAGAC+AGG - chr5.1:253236-253255 None:intergenic 50.0%
!! CCAAGCCCTCAAGAGCATTT+GGG + chr5.1:252722-252741 MS.gene92366:CDS 50.0%
AGCCATACGCAACGAAGGTG+TGG - chr5.1:252568-252587 None:intergenic 55.0%
AGTGGAGCCATACGCAACGA+AGG - chr5.1:252573-252592 None:intergenic 55.0%
CCCAAATGCTCTTGAGGGCT+TGG - chr5.1:252725-252744 None:intergenic 55.0%
GCTTGGGTTCGGGAAATCTC+GGG - chr5.1:252708-252727 None:intergenic 55.0%
GGAGTCCTGAGAGTTAGAGG+AGG - chr5.1:252531-252550 None:intergenic 55.0%
GGCTTGGGTTCGGGAAATCT+CGG - chr5.1:252709-252728 None:intergenic 55.0%
GGTGTGGGAAACGCAAACCT+TGG - chr5.1:252552-252571 None:intergenic 55.0%
GTGGGCCCAAATGCTCTTGA+GGG - chr5.1:252730-252749 None:intergenic 55.0%
TCCCACACCTTCGTTGCGTA+TGG + chr5.1:252563-252582 MS.gene92366:CDS 55.0%
TGCTCTTGAGGGCTTGGGTT+CGG - chr5.1:252719-252738 None:intergenic 55.0%
TGTGGGCCCAAATGCTCTTG+AGG - chr5.1:252731-252750 None:intergenic 55.0%
! GCCATACGCAACGAAGGTGT+GGG - chr5.1:252567-252586 None:intergenic 55.0%
!! CCCAAGCCCTCAAGAGCATT+TGG + chr5.1:252721-252740 MS.gene92366:CDS 55.0%
CCCACCGGAGGCAAAATTCG+CGG + chr5.1:252630-252649 MS.gene92366:CDS 60.0%
CCCGAGATGCAAGACCTCGT+CGG + chr5.1:252678-252697 MS.gene92366:CDS 60.0%
CCGACGAGGTCTTGCATCTC+GGG - chr5.1:252681-252700 None:intergenic 60.0%
CGACGAGGTCTTGCATCTCG+GGG - chr5.1:252680-252699 None:intergenic 60.0%
GACGAGGTCTTGCATCTCGG+GGG - chr5.1:252679-252698 None:intergenic 60.0%
GCCGACGAGGTCTTGCATCT+CGG - chr5.1:252682-252701 None:intergenic 60.0%
GCTCTTGAGGGCTTGGGTTC+GGG - chr5.1:252718-252737 None:intergenic 60.0%
GGGTTCGGGAAATCTCGGGT+TGG - chr5.1:252704-252723 None:intergenic 60.0%
! ACCGCGAATTTTGCCTCCGG+TGG - chr5.1:252634-252653 None:intergenic 60.0%
! ATTTTGCCTCCGGTGGGTGG+AGG - chr5.1:252627-252646 None:intergenic 60.0%
! CCGCGAATTTTGCCTCCGGT+GGG - chr5.1:252633-252652 None:intergenic 60.0%
! CGAATTTTGCCTCCGGTGGG+TGG - chr5.1:252630-252649 None:intergenic 60.0%
!! AAATCTCGGGTTGGCCGACG+AGG - chr5.1:252695-252714 None:intergenic 60.0%
GGTGGGTGGAGGCTGAGAGA+CGG - chr5.1:252616-252635 None:intergenic 65.0%
GTCTCTCAGCCTCCACCCAC+CGG + chr5.1:252615-252634 MS.gene92366:CDS 65.0%
GGGTGGAGGCTGAGAGACGG+TGG - chr5.1:252613-252632 None:intergenic 70.0%
TCTCAGCCTCCACCCACCGG+AGG + chr5.1:252618-252637 MS.gene92366:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 252489 253753 252489 ID=MS.gene92366
chr5.1 mRNA 252489 253753 252489 ID=MS.gene92366.t1;Parent=MS.gene92366
chr5.1 exon 252489 252770 252489 ID=MS.gene92366.t1.exon1;Parent=MS.gene92366.t1
chr5.1 CDS 252489 252770 252489 ID=cds.MS.gene92366.t1;Parent=MS.gene92366.t1
chr5.1 exon 253634 253753 253634 ID=MS.gene92366.t1.exon2;Parent=MS.gene92366.t1
chr5.1 CDS 253634 253753 253634 ID=cds.MS.gene92366.t1;Parent=MS.gene92366.t1
Gene Sequence

>MS.gene92366

ATGGCGGTTTCAATGGCTACCGGACTATTATTATCATCTCCTCCTCTAACTCTCAGGACTCCAAGGTTTGCGTTTCCCACACCTTCGTTGCGTATGGCTCCACTCCGAACAGTTACTTCTGCCACCGTCTCTCAGCCTCCACCCACCGGAGGCAAAATTCGCGGTATTATGAAACCTAAAAAAATCTCCCCCGAGATGCAAGACCTCGTCGGCCAACCCGAGATTTCCCGAACCCAAGCCCTCAAGAGCATTTGGGCCCACATTAAGGAACACAATCTTCAGGTCTAACTTCTCTCCTTTCTCTTTCTCTTGAGGATAATAAGCCTTTTTTAAAATTTCACCTAAACCGTACAAATTATTTGTTTTTGAAATTGTTGTTAAACTGCATAGATTCAGAATGGTAAGTTGTTAGTTATTAAGATGGTTTCATTTCAAGACATGATATCTGTTTCATTGACTACAATACATATATTACAAGACCATTTGTTCATGTTTTATTTTGACTCAACAACCAATTTGGTCTGAATGAATATATTATTAGGCACTAATTTGGGAATAATCGGTTATTATGGGGGCTAAATTCAATTGGGCTGCAGCAGAAAACCAAATCTCGTTTTCATTCTTTTATTTTTAACCAACTAAATGGGAATCCAATTTGTACATCAACTTTTTGACTGTCATATCTAACTCTGTATTTTTAACCTTGTTTGCACTTGTTCTACATACAGCACAATACATAATTAACCTGTCTGTCTGGCCATCAAGTACTAACTCTGCTTTTCCGCGTTTCAATTGCACAAATTTTATGAATTTGTTGTATATGCAATTCTGTTTTATAATGGTTGATGCTGATGGATTCTATTGTCAATTTTGGAGCCATATAATCTAAACTACATGTTTCATATAATTAGATTACAATTCAAGTGTTTATTATTATTATTATTATTATTATTATTATTTTCTAGCTTCCTACAACTACTCTTGTACAGACTACACTAGTATAGTGTTTGATTATTTCCGTATCATCCATAAATTGAATGTTCTCTGGGATAATTATCCATTAATCCAATGCTATCCTGCATTCATAAATACGTTCAGCAACGTTTGATTGGTTTTAATTGAATGTTTCTTTCTAATATATTCAGAACCCTGAGAAAAAGAGGCTTATTCGCTGTGATGAGAAACTGAAGAAGGTATTTGCAGGGAGAGATGAAGTTGATATGCTTGAGATTGCTGGCTTGATTAGCCCTCACTTCCTTAATTGA

Protein sequence

>MS.gene92366.t1

MAVSMATGLLLSSPPLTLRTPRFAFPTPSLRMAPLRTVTSATVSQPPPTGGKIRGIMKPKKISPEMQDLVGQPEISRTQALKSIWAHIKEHNLQNPEKKRLIRCDEKLKKVFAGRDEVDMLEIAGLISPHFLN