AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36317


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36317.t1 MTR_2g101820 97.279 147 3 1 1 146 1 147 5.34e-99 281
MS.gene36317.t1 MTR_2g101820 95.455 88 3 1 1 87 1 88 3.48e-55 169
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36317.t1 AT1G52230 79.452 146 29 1 1 146 1 145 2.10e-81 236
MS.gene36317.t1 AT3G16140 78.082 146 31 1 1 146 1 145 7.76e-80 232

Find 45 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCAAATCCAAGAATTTA+AGG 0.134528 2.2:-1915971 MS.gene36317:intron
GTGAAAGTACCTTAAATTCT+TGG 0.244082 2.2:+1915962 None:intergenic
CTTGCTGGCAGTTCCATTAC+TGG 0.265222 2.2:-1916026 MS.gene36317:CDS
TACTTCAGTGCCACAGCTTC+AGG 0.317013 2.2:-1914722 MS.gene36317:CDS
TACTTTGATTTGGAGGATAT+TGG 0.323961 2.2:-1915643 MS.gene36317:CDS
CAAGAGTGTGTACTTTGATT+TGG 0.336220 2.2:-1915653 MS.gene36317:CDS
TTGCCACGGGGACCGAGCTT+TGG 0.360365 2.2:+1914662 None:intergenic
CTCAATGTTAAGGGTCTTGC+TGG 0.365303 2.2:-1916041 MS.gene36317:CDS
CTCACCATACAACCCTCTTC+AGG 0.381984 2.2:-1915578 MS.gene36317:intron
ACCAGCTGGTCTCAATGTTA+AGG 0.384673 2.2:-1916051 MS.gene36317:CDS
CCACGGGGACCGAGCTTTGG+TGG 0.404075 2.2:+1914665 None:intergenic
GGCTCTGTGGTAGCAAAGTA+TGG 0.420308 2.2:-1915679 MS.gene36317:CDS
AGCTGTGGACCTTTCTTGAT+TGG 0.426964 2.2:+1914689 None:intergenic
CCAGCTGGTCTCAATGTTAA+GGG 0.447321 2.2:-1916050 MS.gene36317:CDS
CAAACCTGAAGAGGGTTGTA+TGG 0.465891 2.2:+1915574 None:intergenic
GAGTGTGTACTTTGATTTGG+AGG 0.467087 2.2:-1915650 MS.gene36317:CDS
GAGGATATTGGCAACACTAC+AGG 0.483887 2.2:-1915631 MS.gene36317:CDS
AATTCTTGGATTTGAAACTC+TGG 0.485960 2.2:+1915976 None:intergenic
CAAGAGTTGAACCACCTCCT+AGG 0.515089 2.2:+1914747 None:intergenic
AATAATCCTCTCTTTGTGAA+TGG 0.515909 2.2:+1914785 None:intergenic
CCCTTAACATTGAGACCAGC+TGG 0.523582 2.2:+1916050 None:intergenic
CCGAGCTTTGGTGGAAGCTG+TGG 0.524654 2.2:+1914674 None:intergenic
GACATTCTACCAATCAAGAA+AGG 0.540823 2.2:-1914698 MS.gene36317:CDS
AGATAGCTTAGTTCCAGTAA+TGG 0.548416 2.2:+1916013 None:intergenic
AGCTGGTTGTACACAAGCTA+AGG 0.551993 2.2:+1916067 None:intergenic
GCAGCTCCATTCACAAAGAG+AGG 0.555627 2.2:-1914791 MS.gene36317:CDS
TCCAGTAAACAAACCTGAAG+AGG 0.556418 2.2:+1915565 None:intergenic
ACAGGTCAGTGGGATTTGTA+CGG 0.573529 2.2:-1915613 MS.gene36317:CDS
TTAGCTTGTGTACAACCAGC+TGG 0.579471 2.2:-1916065 MS.gene36317:CDS
GTACACAAGCTAAGGTAGCA+AGG 0.579499 2.2:+1916075 None:intergenic
GATTTGAAACTCTGGCGAGA+GGG 0.583566 2.2:+1915984 None:intergenic
CCACAGCTTCCACCAAAGCT+CGG 0.583979 2.2:-1914674 MS.gene36317:CDS
AACTAAATTAGATCTTGCCA+CGG 0.594009 2.2:+1914648 None:intergenic
TGGCAACACTACAGGTCAGT+GGG 0.596869 2.2:-1915623 MS.gene36317:CDS
ACTAAATTAGATCTTGCCAC+GGG 0.597169 2.2:+1914649 None:intergenic
GGATTTGAAACTCTGGCGAG+AGG 0.599558 2.2:+1915983 None:intergenic
CCAGTAAACAAACCTGAAGA+GGG 0.604276 2.2:+1915566 None:intergenic
TACACAAGCTAAGGTAGCAA+GGG 0.622521 2.2:+1916076 None:intergenic
TGAAGAGGGTTGTATGGTGA+GGG 0.652580 2.2:+1915580 None:intergenic
AACTTGCAGGAGTGGCTCTG+TGG 0.654086 2.2:-1915692 MS.gene36317:intron
CCACCAAAGCTCGGTCCCCG+TGG 0.657534 2.2:-1914665 MS.gene36317:CDS
TTGGCAACACTACAGGTCAG+TGG 0.661910 2.2:-1915624 MS.gene36317:CDS
TAGAATGTCACCTGAAGCTG+TGG 0.676047 2.2:+1914712 None:intergenic
CTGAAGAGGGTTGTATGGTG+AGG 0.677728 2.2:+1915579 None:intergenic
CTAAATTAGATCTTGCCACG+GGG 0.688209 2.2:+1914650 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTATGAAAATAACTTA+TGG - chr2.2:1915416-1915435 MS.gene36317:intron 15.0%
!! ATCAAATAAACCAATTAATT+TGG + chr2.2:1915853-1915872 None:intergenic 15.0%
!! TTATATTAAGTGTCTTTATA+AGG - chr2.2:1915513-1915532 MS.gene36317:intron 15.0%
!!! TTTTTTCATACCAAATTAAT+TGG - chr2.2:1915840-1915859 MS.gene36317:intron 15.0%
!! TAGATCAACAAAATAATCTA+TGG + chr2.2:1915289-1915308 None:intergenic 20.0%
!! TGCAAATAACTTATCTAAAT+AGG + chr2.2:1914949-1914968 None:intergenic 20.0%
!!! AAATGTAATTAGTTCTTCAT+TGG - chr2.2:1915689-1915708 MS.gene36317:intron 20.0%
!!! CTTCTTGTTTTCTTATAATA+AGG + chr2.2:1915630-1915649 None:intergenic 20.0%
! AGACATTTAATATGAGAATG+AGG - chr2.2:1915759-1915778 MS.gene36317:intron 25.0%
! GACATTTAATATGAGAATGA+GGG - chr2.2:1915760-1915779 MS.gene36317:intron 25.0%
! GTTTCAAATCCAAGAATTTA+AGG - chr2.2:1914767-1914786 MS.gene36317:CDS 25.0%
!! CTCATTTGTTGATATAAACT+TGG - chr2.2:1915895-1915914 MS.gene36317:intron 25.0%
!! TGTTTTCTTATAATAAGGAC+TGG + chr2.2:1915625-1915644 None:intergenic 25.0%
!!! ACATAACATAATTAACTTGC+AGG - chr2.2:1915033-1915052 MS.gene36317:intron 25.0%
!!! GTTATTTTCATAAGTTTTCC+AGG + chr2.2:1915413-1915432 None:intergenic 25.0%
AATAATCCTCTCTTTGTGAA+TGG + chr2.2:1915956-1915975 None:intergenic 30.0%
AGACACTTAATATAACACAC+AGG + chr2.2:1915508-1915527 None:intergenic 30.0%
AGTGATGTTTATATGTGTCT+AGG - chr2.2:1915253-1915272 MS.gene36317:intron 30.0%
GACACTTAATATAACACACA+GGG + chr2.2:1915507-1915526 None:intergenic 30.0%
! AATTCTTGGATTTGAAACTC+TGG + chr2.2:1914765-1914784 None:intergenic 30.0%
!! GTGAAAGTACCTTAAATTCT+TGG + chr2.2:1914779-1914798 None:intergenic 30.0%
!! TACTTTGATTTGGAGGATAT+TGG - chr2.2:1915095-1915114 MS.gene36317:intron 30.0%
!!! GTATTCGATGCATTGATTTT+TGG - chr2.2:1915797-1915816 MS.gene36317:intron 30.0%
AAAAAACAGACAGACGAGTA+TGG + chr2.2:1914841-1914860 None:intergenic 35.0%
AGATAGCTTAGTTCCAGTAA+TGG + chr2.2:1914728-1914747 None:intergenic 35.0%
GACATTCTACCAATCAAGAA+AGG - chr2.2:1916040-1916059 MS.gene36317:CDS 35.0%
GTTTATATGTGTCTAGGCTT+AGG - chr2.2:1915259-1915278 MS.gene36317:intron 35.0%
TATAGTCTATAGGACAGCAA+AGG + chr2.2:1915482-1915501 None:intergenic 35.0%
! CAAGAGTGTGTACTTTGATT+TGG - chr2.2:1915085-1915104 MS.gene36317:intron 35.0%
! TTGCTCAAGTTTTTGATCCT+AGG - chr2.2:1915974-1915993 MS.gene36317:CDS 35.0%
!! ACATAATTAACTTGCAGGAG+TGG - chr2.2:1915038-1915057 MS.gene36317:intron 35.0%
ATATGTGTCTAGGCTTAGGA+AGG - chr2.2:1915263-1915282 MS.gene36317:intron 40.0%
CACTAGCTACTACTTTAGCA+AGG + chr2.2:1915656-1915675 None:intergenic 40.0%
CCAGTAAACAAACCTGAAGA+GGG + chr2.2:1915175-1915194 None:intergenic 40.0%
TACACAAGCTAAGGTAGCAA+GGG + chr2.2:1914665-1914684 None:intergenic 40.0%
TCCAGTAAACAAACCTGAAG+AGG + chr2.2:1915176-1915195 None:intergenic 40.0%
! AAGTTTTTGATCCTAGGAGG+TGG - chr2.2:1915980-1915999 MS.gene36317:CDS 40.0%
! ACACAGGGAGTATAGTCTAT+AGG + chr2.2:1915492-1915511 None:intergenic 40.0%
! CTCAAGTTTTTGATCCTAGG+AGG - chr2.2:1915977-1915996 MS.gene36317:CDS 40.0%
!! GAGTGTGTACTTTGATTTGG+AGG - chr2.2:1915088-1915107 MS.gene36317:intron 40.0%
!! GCTGTTTCTATAAGCTAACC+TGG - chr2.2:1915392-1915411 MS.gene36317:intron 40.0%
ACAGGTCAGTGGGATTTGTA+CGG - chr2.2:1915125-1915144 MS.gene36317:intron 45.0%
AGCTGGTTGTACACAAGCTA+AGG + chr2.2:1914674-1914693 None:intergenic 45.0%
AGCTGTGGACCTTTCTTGAT+TGG + chr2.2:1916052-1916071 None:intergenic 45.0%
AGTCTATAGGACAGCAAAGG+AGG + chr2.2:1915479-1915498 None:intergenic 45.0%
CAAACCTGAAGAGGGTTGTA+TGG + chr2.2:1915167-1915186 None:intergenic 45.0%
CTCAATGTTAAGGGTCTTGC+TGG - chr2.2:1914697-1914716 MS.gene36317:CDS 45.0%
GAGGATATTGGCAACACTAC+AGG - chr2.2:1915107-1915126 MS.gene36317:intron 45.0%
GTACACAAGCTAAGGTAGCA+AGG + chr2.2:1914666-1914685 None:intergenic 45.0%
TAGAATGTCACCTGAAGCTG+TGG + chr2.2:1916029-1916048 None:intergenic 45.0%
TGAAGAGGGTTGTATGGTGA+GGG + chr2.2:1915161-1915180 None:intergenic 45.0%
! ACCAGCTGGTCTCAATGTTA+AGG - chr2.2:1914687-1914706 MS.gene36317:CDS 45.0%
! CCAGCTGGTCTCAATGTTAA+GGG - chr2.2:1914688-1914707 MS.gene36317:CDS 45.0%
! CCCTCTTCAGGTTTGTTTAC+TGG - chr2.2:1915172-1915191 MS.gene36317:intron 45.0%
! GATTTGAAACTCTGGCGAGA+GGG + chr2.2:1914757-1914776 None:intergenic 45.0%
!! TTAGCTTGTGTACAACCAGC+TGG - chr2.2:1914673-1914692 MS.gene36317:CDS 45.0%
CAAGAGTTGAACCACCTCCT+AGG + chr2.2:1915994-1916013 None:intergenic 50.0%
CCCTTAACATTGAGACCAGC+TGG + chr2.2:1914691-1914710 None:intergenic 50.0%
CTCACCATACAACCCTCTTC+AGG - chr2.2:1915160-1915179 MS.gene36317:intron 50.0%
CTGAAGAGGGTTGTATGGTG+AGG + chr2.2:1915162-1915181 None:intergenic 50.0%
CTTGCTGGCAGTTCCATTAC+TGG - chr2.2:1914712-1914731 MS.gene36317:CDS 50.0%
GCAGCTCCATTCACAAAGAG+AGG - chr2.2:1915947-1915966 MS.gene36317:intron 50.0%
GGCTCTGTGGTAGCAAAGTA+TGG - chr2.2:1915059-1915078 MS.gene36317:intron 50.0%
TACTTCAGTGCCACAGCTTC+AGG - chr2.2:1916016-1916035 MS.gene36317:CDS 50.0%
TGGCAACACTACAGGTCAGT+GGG - chr2.2:1915115-1915134 MS.gene36317:intron 50.0%
TTGGCAACACTACAGGTCAG+TGG - chr2.2:1915114-1915133 MS.gene36317:intron 50.0%
! GGATTTGAAACTCTGGCGAG+AGG + chr2.2:1914758-1914777 None:intergenic 50.0%
CCACAGCTTCCACCAAAGCT+CGG - chr2.2:1916064-1916083 MS.gene36317:CDS 55.0%
! AACTTGCAGGAGTGGCTCTG+TGG - chr2.2:1915046-1915065 MS.gene36317:intron 55.0%
! CCGAGCTTTGGTGGAAGCTG+TGG + chr2.2:1916067-1916086 None:intergenic 60.0%
TTGCCACGGGGACCGAGCTT+TGG + chr2.2:1916079-1916098 None:intergenic 65.0%
CCACCAAAGCTCGGTCCCCG+TGG - chr2.2:1916073-1916092 MS.gene36317:CDS 70.0%
! CCACGGGGACCGAGCTTTGG+TGG + chr2.2:1916076-1916095 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 1914655 1916105 1914655 ID=MS.gene36317
chr2.2 mRNA 1914655 1916105 1914655 ID=MS.gene36317.t1;Parent=MS.gene36317
chr2.2 exon 1915972 1916105 1915972 ID=MS.gene36317.t1.exon1;Parent=MS.gene36317.t1
chr2.2 CDS 1915972 1916105 1915972 ID=cds.MS.gene36317.t1;Parent=MS.gene36317.t1
chr2.2 exon 1915579 1915705 1915579 ID=MS.gene36317.t1.exon2;Parent=MS.gene36317.t1
chr2.2 CDS 1915579 1915705 1915579 ID=cds.MS.gene36317.t1;Parent=MS.gene36317.t1
chr2.2 exon 1914655 1914834 1914655 ID=MS.gene36317.t1.exon3;Parent=MS.gene36317.t1
chr2.2 CDS 1914655 1914834 1914655 ID=cds.MS.gene36317.t1;Parent=MS.gene36317.t1
Gene Sequence

>MS.gene36317

ATGGCTTCCCTTGCTACCTTAGCTTGTGTACAACCAGCTGGTCTCAATGTTAAGGGTCTTGCTGGCAGTTCCATTACTGGAACTAAGCTATCTTTCAAGCCCTCTCGCCAGAGTTTCAAATCCAAGAATTTAAGGTACTTTCACAACTTTGAACAAATTATTTCAATTAATATAACTAGCATGCCATACTCGTCTGTCTGTTTTTTTATTACGCTAATGTTTATGTTTTTAAAGTATAAAGTGTCTGTAGTGCAATTTTTATTTAAGTAAACTTCTTAAGTTATATGAGATCCTATTTAGATAAGTTATTTGCAGTTTATAACACAAACACTTATTTAAATTAAATCAACTCATATTATATGTGTATGTGTAAGCTTAACATAACATAATTAACTTGCAGGAGTGGCTCTGTGGTAGCAAAGTATGGTGACAAGAGTGTGTACTTTGATTTGGAGGATATTGGCAACACTACAGGTCAGTGGGATTTGTACGGCTCAGATGCACCCTCACCATACAACCCTCTTCAGGTTTGTTTACTGGATTTTAGATCACTTTACTTTGTTTTGTGCATTCATTTTGTAGTGAGTAATGAACACAAAGTGATGTTTATATGTGTCTAGGCTTAGGAAGGCCATAGATTATTTTGTTGATCTAGCATAAGCACATATCATATAAGTGTTTCTGTATAAGCTATTTTTATAACAAAAATTAAATAAAGTTAAACTATTTTCATATAAGCTGTTTCTATAAGCTAACCTGGAAAACTTATGAAAATAACTTATGGACAATCAAACATGTTTTCATTATAAGTTCGCTTTAAGCCTCCTTTGCTGTCCTATAGACTATACTCCCTGTGTGTTATATTAAGTGTCTTTATAAGGTTATGCACACTTCTTAAGAAAATTATTGAGTGTACTTATTGATAAAATGAGTTTTACTTACTAAAGTATTCGTATTAGTACTCGCTCCAGTCCTTATTATAAGAAAACAAGAAGTATCCTTGCTAAAGTAGTAGCTAGTGTATATTAGTAGAAAAATGTAATTAGTTCTTCATTGGTATTCTAAAACGACAAAAAAATGTGAGACAAACAAATATTTTAATTGAGACATTTAATATGAGAATGAGGGAGTAGTATATTCGTGTATTCGATGCATTGATTTTTGGCAGTAAAATGTTTTTTTTTTTTTTTTCATACCAAATTAATTGGTTTATTTGATTGAAAATGCACAAACTTGATCTCTCATTTGTTGATATAAACTTGGCATTGCAGAGCAAGTTCTTTGAGACATTTGCAGCTCCATTCACAAAGAGAGGATTATTGCTCAAGTTTTTGATCCTAGGAGGTGGTTCAACTCTTGCATACTTCAGTGCCACAGCTTCAGGTGACATTCTACCAATCAAGAAAGGTCCACAGCTTCCACCAAAGCTCGGTCCCCGTGGCAAGATCTAA

Protein sequence

>MS.gene36317.t1

MASLATLACVQPAGLNVKGLAGSSITGTKLSFKPSRQSFKSKNLRSGSVVAKYGDKSVYFDLEDIGNTTGQWDLYGSDAPSPYNPLQSKFFETFAAPFTKRGLLLKFLILGGGSTLAYFSATASGDILPIKKGPQLPPKLGPRGKI