AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85117


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85117.t1 MTR_2g101820 97.279 147 3 1 1 146 1 147 5.34e-99 281
MS.gene85117.t1 MTR_2g101820 95.455 88 3 1 1 87 1 88 3.48e-55 169
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85117.t1 AT1G52230 79.452 146 29 1 1 146 1 145 2.10e-81 236
MS.gene85117.t1 AT3G16140 78.082 146 31 1 1 146 1 145 7.76e-80 232

Find 45 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCAAATCCAAGAATTTA+AGG 0.134528 2.4:-3062543 MS.gene85117:intron
GTGAAAGTACCTTAAATTCT+TGG 0.244082 2.4:+3062534 None:intergenic
CTTGCTGGCAGTTCCATTAC+TGG 0.265222 2.4:-3062598 MS.gene85117:CDS
TACTTCAGTGCCACAGCTTC+AGG 0.317013 2.4:-3061275 MS.gene85117:CDS
TACTTTGATTTGGAGGATAT+TGG 0.323961 2.4:-3062209 MS.gene85117:CDS
CAAGAGTGTGTACTTTGATT+TGG 0.336220 2.4:-3062219 MS.gene85117:CDS
TTGCCACGGGGACCGAGCTT+TGG 0.360365 2.4:+3061215 None:intergenic
CTCAATGTTAAGGGTCTTGC+TGG 0.365303 2.4:-3062613 MS.gene85117:CDS
CTCACCATACAACCCTCTTC+AGG 0.381984 2.4:-3062144 MS.gene85117:intron
ACCAGCTGGTCTCAATGTTA+AGG 0.384673 2.4:-3062623 MS.gene85117:CDS
CCACGGGGACCGAGCTTTGG+TGG 0.404075 2.4:+3061218 None:intergenic
GGCTCTGTGGTAGCAAAGTA+TGG 0.420308 2.4:-3062245 MS.gene85117:CDS
AGCTGTGGACCTTTCTTGAT+TGG 0.426964 2.4:+3061242 None:intergenic
CCAGCTGGTCTCAATGTTAA+GGG 0.447321 2.4:-3062622 MS.gene85117:CDS
GAGTGTGTACTTTGATTTGG+AGG 0.467087 2.4:-3062216 MS.gene85117:CDS
GAGGATATTGGCAACACTAC+AGG 0.483887 2.4:-3062197 MS.gene85117:CDS
AATTCTTGGATTTGAAACTC+TGG 0.485960 2.4:+3062548 None:intergenic
CAAACCTGAAGAGGGTTGTA+TGG 0.501808 2.4:+3062140 None:intergenic
CAAGAGTTGAACCACCTCCT+AGG 0.515089 2.4:+3061300 None:intergenic
AATAATCCTCTCTTTGTGAA+TGG 0.515909 2.4:+3061338 None:intergenic
CCCTTAACATTGAGACCAGC+TGG 0.523582 2.4:+3062622 None:intergenic
CCGAGCTTTGGTGGAAGCTG+TGG 0.524654 2.4:+3061227 None:intergenic
GACATTCTACCAATCAAGAA+AGG 0.540823 2.4:-3061251 MS.gene85117:CDS
AGATAGCTTAGTTCCAGTAA+TGG 0.548416 2.4:+3062585 None:intergenic
AGCTGGTTGTACACAAGCTA+AGG 0.551993 2.4:+3062639 None:intergenic
GCAGCTCCATTCACAAAGAG+AGG 0.555627 2.4:-3061344 MS.gene85117:CDS
TCCAGTAGACAAACCTGAAG+AGG 0.558714 2.4:+3062131 None:intergenic
ACAGGTCAGTGGGATTTGTA+CGG 0.573529 2.4:-3062179 MS.gene85117:CDS
TTAGCTTGTGTACAACCAGC+TGG 0.579471 2.4:-3062637 MS.gene85117:CDS
GTACACAAGCTAAGGTAGCA+AGG 0.579499 2.4:+3062647 None:intergenic
GATTTGAAACTCTGGCGAGA+GGG 0.583566 2.4:+3062556 None:intergenic
CCACAGCTTCCACCAAAGCT+CGG 0.583979 2.4:-3061227 MS.gene85117:CDS
TGGCAACACTACAGGTCAGT+GGG 0.596869 2.4:-3062189 MS.gene85117:CDS
AACTAGATTAGATCTTGCCA+CGG 0.597660 2.4:+3061201 None:intergenic
GGATTTGAAACTCTGGCGAG+AGG 0.599558 2.4:+3062555 None:intergenic
ACTAGATTAGATCTTGCCAC+GGG 0.603116 2.4:+3061202 None:intergenic
CCAGTAGACAAACCTGAAGA+GGG 0.614925 2.4:+3062132 None:intergenic
TACACAAGCTAAGGTAGCAA+GGG 0.622521 2.4:+3062648 None:intergenic
TGAAGAGGGTTGTATGGTGA+GGG 0.652580 2.4:+3062146 None:intergenic
AACTTGCAGGAGTGGCTCTG+TGG 0.654086 2.4:-3062258 MS.gene85117:intron
CCACCAAAGCTCGGTCCCCG+TGG 0.657534 2.4:-3061218 MS.gene85117:CDS
TTGGCAACACTACAGGTCAG+TGG 0.661910 2.4:-3062190 MS.gene85117:CDS
TAGAATGTCACCTGAAGCTG+TGG 0.676047 2.4:+3061265 None:intergenic
CTGAAGAGGGTTGTATGGTG+AGG 0.677728 2.4:+3062145 None:intergenic
CTAGATTAGATCTTGCCACG+GGG 0.731168 2.4:+3061203 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAACTTATCTAATCTAAAT+AGG + chr2.4:3061503-3061522 None:intergenic 15.0%
!! ATCAAAGAAATCAATTAATT+TGG + chr2.4:3062425-3062444 None:intergenic 15.0%
!! TTATATTAAGTGTCTTTATA+AGG - chr2.4:3062083-3062102 MS.gene85117:intron 15.0%
!! AATAAGCTGAAAATAACTTA+TGG - chr2.4:3061986-3062005 MS.gene85117:intron 20.0%
!! CATTAGCGTAATAAAAAAAA+CGG + chr2.4:3061408-3061427 None:intergenic 20.0%
!! TAGATCAACAAAATAATCTA+TGG + chr2.4:3061848-3061867 None:intergenic 20.0%
!!! AAATGTAATTAGTTCTTCAT+TGG - chr2.4:3062259-3062278 MS.gene85117:intron 20.0%
!!! CTTCTTGTTTTCTTATAATA+AGG + chr2.4:3062200-3062219 None:intergenic 20.0%
! AGACATTTAATATGAGAATG+AGG - chr2.4:3062329-3062348 MS.gene85117:intron 25.0%
! GACATTTAATATGAGAATGA+GGG - chr2.4:3062330-3062349 MS.gene85117:intron 25.0%
! GTTTCAAATCCAAGAATTTA+AGG - chr2.4:3061320-3061339 MS.gene85117:CDS 25.0%
!! CTCATTTGTTGATATAAACT+TGG - chr2.4:3062467-3062486 MS.gene85117:intron 25.0%
!! TGCTGTAACAAATTGATTTA+TGG - chr2.4:3062375-3062394 MS.gene85117:intron 25.0%
!! TGTTTTCTTATAATAAGGAC+TGG + chr2.4:3062195-3062214 None:intergenic 25.0%
!!! ACATAACATAATTAACTTGC+AGG - chr2.4:3061592-3061611 MS.gene85117:intron 25.0%
!!! CTTATTTTCATAAGTTTTCC+AGG + chr2.4:3061972-3061991 None:intergenic 25.0%
AATAATCCTCTCTTTGTGAA+TGG + chr2.4:3062528-3062547 None:intergenic 30.0%
AGACACTTAATATAACACAG+AGG + chr2.4:3062078-3062097 None:intergenic 30.0%
AGTGATGTTTATATGTGTCT+AGG - chr2.4:3061812-3061831 MS.gene85117:intron 30.0%
GACACTTAATATAACACAGA+GGG + chr2.4:3062077-3062096 None:intergenic 30.0%
! AATTCTTGGATTTGAAACTC+TGG + chr2.4:3061318-3061337 None:intergenic 30.0%
!! GTGAAAGTACCTTAAATTCT+TGG + chr2.4:3061332-3061351 None:intergenic 30.0%
!! TACTTTGATTTGGAGGATAT+TGG - chr2.4:3061654-3061673 MS.gene85117:intron 30.0%
AGATAGCTTAGTTCCAGTAA+TGG + chr2.4:3061281-3061300 None:intergenic 35.0%
GACATTCTACCAATCAAGAA+AGG - chr2.4:3062612-3062631 MS.gene85117:CDS 35.0%
GTTTATATGTGTCTAGGCTT+AGG - chr2.4:3061818-3061837 MS.gene85117:intron 35.0%
TATAGTCTATAGGACAGCAA+AGG + chr2.4:3062052-3062071 None:intergenic 35.0%
! CAAGAGTGTGTACTTTGATT+TGG - chr2.4:3061644-3061663 MS.gene85117:intron 35.0%
! TTGCTCAAGTTTTTGATCCT+AGG - chr2.4:3062546-3062565 MS.gene85117:CDS 35.0%
!! ACATAATTAACTTGCAGGAG+TGG - chr2.4:3061597-3061616 MS.gene85117:intron 35.0%
AAAAAACGGACAGACGAGTA+TGG + chr2.4:3061394-3061413 None:intergenic 40.0%
ATATGTGTCTAGGCTTAGGA+AGG - chr2.4:3061822-3061841 MS.gene85117:intron 40.0%
CACTAGCTACTACTTTAGCA+AGG + chr2.4:3062226-3062245 None:intergenic 40.0%
TACACAAGCTAAGGTAGCAA+GGG + chr2.4:3061218-3061237 None:intergenic 40.0%
! AAGTTTTTGATCCTAGGAGG+TGG - chr2.4:3062552-3062571 MS.gene85117:CDS 40.0%
! ACAGAGGGAGTATAGTCTAT+AGG + chr2.4:3062062-3062081 None:intergenic 40.0%
! CTCAAGTTTTTGATCCTAGG+AGG - chr2.4:3062549-3062568 MS.gene85117:CDS 40.0%
!! GAGTGTGTACTTTGATTTGG+AGG - chr2.4:3061647-3061666 MS.gene85117:intron 40.0%
!! GCTGTTTCTATAAGCTAACC+TGG - chr2.4:3061951-3061970 MS.gene85117:intron 40.0%
ACAGGTCAGTGGGATTTGTA+CGG - chr2.4:3061684-3061703 MS.gene85117:intron 45.0%
AGCTGGTTGTACACAAGCTA+AGG + chr2.4:3061227-3061246 None:intergenic 45.0%
AGCTGTGGACCTTTCTTGAT+TGG + chr2.4:3062624-3062643 None:intergenic 45.0%
AGTCTATAGGACAGCAAAGG+AGG + chr2.4:3062049-3062068 None:intergenic 45.0%
CAAACCTGAAGAGGGTTGTA+TGG + chr2.4:3061726-3061745 None:intergenic 45.0%
CCAGTAGACAAACCTGAAGA+GGG + chr2.4:3061734-3061753 None:intergenic 45.0%
CTCAATGTTAAGGGTCTTGC+TGG - chr2.4:3061250-3061269 MS.gene85117:CDS 45.0%
GAGGATATTGGCAACACTAC+AGG - chr2.4:3061666-3061685 MS.gene85117:intron 45.0%
GTACACAAGCTAAGGTAGCA+AGG + chr2.4:3061219-3061238 None:intergenic 45.0%
TAGAATGTCACCTGAAGCTG+TGG + chr2.4:3062601-3062620 None:intergenic 45.0%
TCCAGTAGACAAACCTGAAG+AGG + chr2.4:3061735-3061754 None:intergenic 45.0%
TGAAGAGGGTTGTATGGTGA+GGG + chr2.4:3061720-3061739 None:intergenic 45.0%
! ACCAGCTGGTCTCAATGTTA+AGG - chr2.4:3061240-3061259 MS.gene85117:CDS 45.0%
! CCAGCTGGTCTCAATGTTAA+GGG - chr2.4:3061241-3061260 MS.gene85117:CDS 45.0%
! GATTTGAAACTCTGGCGAGA+GGG + chr2.4:3061310-3061329 None:intergenic 45.0%
!! TTAGCTTGTGTACAACCAGC+TGG - chr2.4:3061226-3061245 MS.gene85117:CDS 45.0%
CAAGAGTTGAACCACCTCCT+AGG + chr2.4:3062566-3062585 None:intergenic 50.0%
CCCTTAACATTGAGACCAGC+TGG + chr2.4:3061244-3061263 None:intergenic 50.0%
CTCACCATACAACCCTCTTC+AGG - chr2.4:3061719-3061738 MS.gene85117:intron 50.0%
CTGAAGAGGGTTGTATGGTG+AGG + chr2.4:3061721-3061740 None:intergenic 50.0%
CTTGCTGGCAGTTCCATTAC+TGG - chr2.4:3061265-3061284 MS.gene85117:CDS 50.0%
GCAGCTCCATTCACAAAGAG+AGG - chr2.4:3062519-3062538 MS.gene85117:intron 50.0%
GGCTCTGTGGTAGCAAAGTA+TGG - chr2.4:3061618-3061637 MS.gene85117:intron 50.0%
TACTTCAGTGCCACAGCTTC+AGG - chr2.4:3062588-3062607 MS.gene85117:CDS 50.0%
TGGCAACACTACAGGTCAGT+GGG - chr2.4:3061674-3061693 MS.gene85117:intron 50.0%
TTGGCAACACTACAGGTCAG+TGG - chr2.4:3061673-3061692 MS.gene85117:intron 50.0%
! CCCTCTTCAGGTTTGTCTAC+TGG - chr2.4:3061731-3061750 MS.gene85117:intron 50.0%
! GGATTTGAAACTCTGGCGAG+AGG + chr2.4:3061311-3061330 None:intergenic 50.0%
CCACAGCTTCCACCAAAGCT+CGG - chr2.4:3062636-3062655 MS.gene85117:CDS 55.0%
! AACTTGCAGGAGTGGCTCTG+TGG - chr2.4:3061605-3061624 MS.gene85117:intron 55.0%
! CCGAGCTTTGGTGGAAGCTG+TGG + chr2.4:3062639-3062658 None:intergenic 60.0%
TTGCCACGGGGACCGAGCTT+TGG + chr2.4:3062651-3062670 None:intergenic 65.0%
CCACCAAAGCTCGGTCCCCG+TGG - chr2.4:3062645-3062664 MS.gene85117:CDS 70.0%
! CCACGGGGACCGAGCTTTGG+TGG + chr2.4:3062648-3062667 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 3061208 3062677 3061208 ID=MS.gene85117
chr2.4 mRNA 3061208 3062677 3061208 ID=MS.gene85117.t1;Parent=MS.gene85117
chr2.4 exon 3062544 3062677 3062544 ID=MS.gene85117.t1.exon1;Parent=MS.gene85117.t1
chr2.4 CDS 3062544 3062677 3062544 ID=cds.MS.gene85117.t1;Parent=MS.gene85117.t1
chr2.4 exon 3062145 3062271 3062145 ID=MS.gene85117.t1.exon2;Parent=MS.gene85117.t1
chr2.4 CDS 3062145 3062271 3062145 ID=cds.MS.gene85117.t1;Parent=MS.gene85117.t1
chr2.4 exon 3061208 3061387 3061208 ID=MS.gene85117.t1.exon3;Parent=MS.gene85117.t1
chr2.4 CDS 3061208 3061387 3061208 ID=cds.MS.gene85117.t1;Parent=MS.gene85117.t1
Gene Sequence

>MS.gene85117

ATGGCTTCCCTTGCTACCTTAGCTTGTGTACAACCAGCTGGTCTCAATGTTAAGGGTCTTGCTGGCAGTTCCATTACTGGAACTAAGCTATCTTTCAAGCCCTCTCGCCAGAGTTTCAAATCCAAGAATTTAAGGTACTTTCACAACTTTGAACTAATTATTTCAATTAATATAACTAGCATGCCATACTCGTCTGTCCGTTTTTTTTATTACGCTAATGTTTATGTTTTTAAAGTATAAAGTGTTTGTAGTGCAATTTTTATTTAAGTAAACTTCTTAAGTTATATGAGATCCTATTTAGATTAGATAAGTTATTTGCAGTTTATAACACAAACACTTATTTAAATTAAATCAACTCATATTATATGTGTATGTGTAAGCTTAACATAACATAATTAACTTGCAGGAGTGGCTCTGTGGTAGCAAAGTATGGTGACAAGAGTGTGTACTTTGATTTGGAGGATATTGGCAACACTACAGGTCAGTGGGATTTGTACGGCTCAGATGCACCCTCACCATACAACCCTCTTCAGGTTTGTCTACTGGATTTTAGATCACTTTACTTTGTTTTGTGCATTCATTTAGTAGTGAGTAATGAACACAAAGTGATGTTTATATGTGTCTAGGCTTAGGAAGGCCATAGATTATTTTGTTGATCTAGCATAAGCACATATCATATAAGTGTTTCTGTATAAGCTATTTTTATAACAAAAATTAAATAAAGTTAAACTATTTTCATATAAGCTGTTTCTATAAGCTAACCTGGAAAACTTATGAAAATAAGCTGAAAATAACTTATGGACAATCAAACATGTTTTCATTATAAGTTCGCTTTAAGCCTCCTTTGCTGTCCTATAGACTATACTCCCTCTGTGTTATATTAAGTGTCTTTATAAGGTTATGCACACTTCTTAAGAAAATCATTGAGTGTACTTATTGATAAAATGAGTTTTACTTACTAAAGTATTCGTATTAGTACTCGCTCCAGTCCTTATTATAAGAAAACAAGAAGTATCCTTGCTAAAGTAGTAGCTAGTGTATATTAGTAGAAAAATGTAATTAGTTCTTCATTGGTATTCTAAAACGACAAAAAAATGTGAGACAAACAAATATTTCAAATGAGACATTTAATATGAGAATGAGGGAGTAGTATATTAGTGTATTCGATGCTGTAACAAATTGATTTATGGCAGCAAAATGTTTTTTTTTTCATACCAAATTAATTGATTTCTTTGATTGAAAATGCACAAACTTGATCTCTCATTTGTTGATATAAACTTGGCATTGCAGAGCAAGTTCTTTGAGACATTTGCAGCTCCATTCACAAAGAGAGGATTATTGCTCAAGTTTTTGATCCTAGGAGGTGGTTCAACTCTTGCATACTTCAGTGCCACAGCTTCAGGTGACATTCTACCAATCAAGAAAGGTCCACAGCTTCCACCAAAGCTCGGTCCCCGTGGCAAGATCTAA

Protein sequence

>MS.gene85117.t1

MASLATLACVQPAGLNVKGLAGSSITGTKLSFKPSRQSFKSKNLRSGSVVAKYGDKSVYFDLEDIGNTTGQWDLYGSDAPSPYNPLQSKFFETFAAPFTKRGLLLKFLILGGGSTLAYFSATASGDILPIKKGPQLPPKLGPRGKI