AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065537


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065537.t1 MTR_5g093240 90.164 122 9 1 1 122 1 119 2.60e-76 221
MS.gene065537.t1 MTR_5g093240 84.884 86 10 1 1 86 1 83 1.21e-45 143
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTCAGTAAGTTCATTA+AGG 0.284385 5.1:-74392906 MS.gene065537:CDS
CTTCTGCATCATCAACCTTC+TGG 0.304356 5.1:+74392569 None:intergenic
CGCAGCAGCAACATTATCTA+AGG 0.361910 5.1:-74392993 MS.gene065537:CDS
GCAGCAACAACAATGATATC+TGG 0.364461 5.1:+74392871 None:intergenic
GCACAACACAAAACTACTTT+GGG 0.366214 5.1:-74392965 MS.gene065537:CDS
ATGGATCGGCGGGTTGTGGC+AGG 0.389363 5.1:+74392842 None:intergenic
AAATACTTGCTTATAAAATC+TGG 0.401289 5.1:+74392616 None:intergenic
GAAGCAATGAAGAGGGGAGG+TGG 0.407582 5.1:+74393037 None:intergenic
CTATGTGGCATGGTCTTGCT+TGG 0.417895 5.1:+74392937 None:intergenic
AGCACAACACAAAACTACTT+TGG 0.452055 5.1:-74392966 MS.gene065537:CDS
CAATGATATCTGGAATTGCC+TGG 0.462076 5.1:+74392881 None:intergenic
TGAAGTATACTGATATAAGC+AGG 0.464588 5.1:+74392656 None:intergenic
CTTACTCGGAGAAAAGTAGA+TGG 0.479013 5.1:+74392823 None:intergenic
GTAGATGGATCGGCGGGTTG+TGG 0.490348 5.1:+74392838 None:intergenic
GAAAAGAACCGAACCTTACT+CGG 0.537160 5.1:+74392809 None:intergenic
CTTAGATAATGTTGCTGCTG+CGG 0.540896 5.1:+74392994 None:intergenic
TTTGGATTTGATAAACTAGA+GGG 0.553590 5.1:-74392595 MS.gene065537:CDS
TGCATGAGAAGCAATGAAGA+GGG 0.563172 5.1:+74393030 None:intergenic
TGGATCGGCGGGTTGTGGCA+GGG 0.568272 5.1:+74392843 None:intergenic
AATGATATCTGGAATTGCCT+GGG 0.588413 5.1:+74392882 None:intergenic
GAAGAAAAGTGCTTACTATG+TGG 0.588606 5.1:+74392922 None:intergenic
AGAAAAGTAGATGGATCGGC+GGG 0.589351 5.1:+74392832 None:intergenic
GCAGCAACATTATCTAAGGA+TGG 0.606428 5.1:-74392989 MS.gene065537:CDS
AGTAAGTTCATTAAGGACCC+AGG 0.609846 5.1:-74392899 MS.gene065537:CDS
TCGGAGAAAAGTAGATGGAT+CGG 0.611697 5.1:+74392828 None:intergenic
TAAACTAGAGGGAGACCAGA+AGG 0.616294 5.1:-74392584 MS.gene065537:CDS
AAAGTGCTTACTATGTGGCA+TGG 0.619717 5.1:+74392927 None:intergenic
GAAGTATACTGATATAAGCA+GGG 0.632422 5.1:+74392657 None:intergenic
GGGGAGGTGGAGACAAGTCA+TGG 0.642574 5.1:+74393050 None:intergenic
ATGCATGAGAAGCAATGAAG+AGG 0.647708 5.1:+74393029 None:intergenic
TGAGAAGCAATGAAGAGGGG+AGG 0.663602 5.1:+74393034 None:intergenic
GAGAAAAGTAGATGGATCGG+CGG 0.667923 5.1:+74392831 None:intergenic
GCATGAGAAGCAATGAAGAG+GGG 0.705476 5.1:+74393031 None:intergenic
CACAACACAAAACTACTTTG+GGG 0.712340 5.1:-74392964 MS.gene065537:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTATTTATTTTTTTACACT+AGG - chr5.1:74392862-74392881 MS.gene065537:CDS 15.0%
!! AAATACTTGCTTATAAAATC+TGG + chr5.1:74392996-74393015 None:intergenic 20.0%
!!! GATTTTATAAGCAAGTATTT+TGG - chr5.1:74392996-74393015 MS.gene065537:CDS 20.0%
! GTTCATTATGTCATATTTCA+TGG - chr5.1:74392831-74392850 MS.gene065537:CDS 25.0%
! TTTCTTCAGTAAGTTCATTA+AGG - chr5.1:74392703-74392722 MS.gene065537:intron 25.0%
! TTTGGATTTGATAAACTAGA+GGG - chr5.1:74393014-74393033 MS.gene065537:CDS 25.0%
!! TTTTGGATTTGATAAACTAG+AGG - chr5.1:74393013-74393032 MS.gene065537:CDS 25.0%
GAAGTATACTGATATAAGCA+GGG + chr5.1:74392955-74392974 None:intergenic 30.0%
TGAAGTATACTGATATAAGC+AGG + chr5.1:74392956-74392975 None:intergenic 30.0%
ACAAGCTGCAAACTTAAACA+AGG - chr5.1:74392892-74392911 MS.gene065537:CDS 35.0%
AGCACAACACAAAACTACTT+TGG - chr5.1:74392643-74392662 MS.gene065537:CDS 35.0%
CACAACACAAAACTACTTTG+GGG - chr5.1:74392645-74392664 MS.gene065537:CDS 35.0%
GCACAACACAAAACTACTTT+GGG - chr5.1:74392644-74392663 MS.gene065537:CDS 35.0%
! AATGATATCTGGAATTGCCT+GGG + chr5.1:74392730-74392749 None:intergenic 35.0%
!! GAAGAAAAGTGCTTACTATG+TGG + chr5.1:74392690-74392709 None:intergenic 35.0%
AGTAAGTTCATTAAGGACCC+AGG - chr5.1:74392710-74392729 MS.gene065537:intron 40.0%
ATGCATGAGAAGCAATGAAG+AGG + chr5.1:74392583-74392602 None:intergenic 40.0%
CTTACTCGGAGAAAAGTAGA+TGG + chr5.1:74392789-74392808 None:intergenic 40.0%
CTTAGATAATGTTGCTGCTG+CGG + chr5.1:74392618-74392637 None:intergenic 40.0%
GAAAAGAACCGAACCTTACT+CGG + chr5.1:74392803-74392822 None:intergenic 40.0%
GCAGCAACAACAATGATATC+TGG + chr5.1:74392741-74392760 None:intergenic 40.0%
GCAGCAACATTATCTAAGGA+TGG - chr5.1:74392620-74392639 MS.gene065537:CDS 40.0%
TCGGAGAAAAGTAGATGGAT+CGG + chr5.1:74392784-74392803 None:intergenic 40.0%
TGCATGAGAAGCAATGAAGA+GGG + chr5.1:74392582-74392601 None:intergenic 40.0%
! AAAGTGCTTACTATGTGGCA+TGG + chr5.1:74392685-74392704 None:intergenic 40.0%
! ACTTTTCTCCGAGTAAGGTT+CGG - chr5.1:74392792-74392811 MS.gene065537:intron 40.0%
! CAATGATATCTGGAATTGCC+TGG + chr5.1:74392731-74392750 None:intergenic 40.0%
!! CATCTACTTTTCTCCGAGTA+AGG - chr5.1:74392787-74392806 MS.gene065537:intron 40.0%
AGAAAAGTAGATGGATCGGC+GGG + chr5.1:74392780-74392799 None:intergenic 45.0%
CGCAGCAGCAACATTATCTA+AGG - chr5.1:74392616-74392635 MS.gene065537:CDS 45.0%
CTTCTGCATCATCAACCTTC+TGG + chr5.1:74393043-74393062 None:intergenic 45.0%
GAGAAAAGTAGATGGATCGG+CGG + chr5.1:74392781-74392800 None:intergenic 45.0%
GCATGAGAAGCAATGAAGAG+GGG + chr5.1:74392581-74392600 None:intergenic 45.0%
TAAACTAGAGGGAGACCAGA+AGG - chr5.1:74393025-74393044 MS.gene065537:CDS 45.0%
TGAGAAGCAATGAAGAGGGG+AGG + chr5.1:74392578-74392597 None:intergenic 50.0%
! CTATGTGGCATGGTCTTGCT+TGG + chr5.1:74392675-74392694 None:intergenic 50.0%
GAAGCAATGAAGAGGGGAGG+TGG + chr5.1:74392575-74392594 None:intergenic 55.0%
!! GTAGATGGATCGGCGGGTTG+TGG + chr5.1:74392774-74392793 None:intergenic 60.0%
!! ATGGATCGGCGGGTTGTGGC+AGG + chr5.1:74392770-74392789 None:intergenic 65.0%
!! TGGATCGGCGGGTTGTGGCA+GGG + chr5.1:74392769-74392788 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 74392561 74393070 74392561 ID=MS.gene065537
chr5.1 mRNA 74392561 74393070 74392561 ID=MS.gene065537.t1;Parent=MS.gene065537
chr5.1 exon 74392823 74393070 74392823 ID=MS.gene065537.t1.exon1;Parent=MS.gene065537.t1
chr5.1 CDS 74392823 74393070 74392823 ID=cds.MS.gene065537.t1;Parent=MS.gene065537.t1
chr5.1 exon 74392561 74392681 74392561 ID=MS.gene065537.t1.exon2;Parent=MS.gene065537.t1
chr5.1 CDS 74392561 74392681 74392561 ID=cds.MS.gene065537.t1;Parent=MS.gene065537.t1
Gene Sequence

>MS.gene065537

ATGACTTGTCTCCACCTCCCCTCTTCATTGCTTCTCATGCATACAAAACTCAAACCGCAGCAGCAACATTATCTAAGGATGGAGCACAACACAAAACTACTTTGGGGAGAACCAAGCAAGACCATGCCACATAGTAAGCACTTTTCTTCAGTAAGTTCATTAAGGACCCAGGCAATTCCAGATATCATTGTTGTTGCTGCTACTTCCCTGCCACAACCCGCCGATCCATCTACTTTTCTCCGAGTAAGGTTCGGTTCTTTTCTTTCACTTGTTCATTATGTCATATTTCATGGTCATGTATAGTATTTATTTTTTTACACTAGGAGATATAACAAGCTGCAAACTTAAACAAGGTTGTTTTCTGCTCATAATTTGTTTATACATTGCAGTGCCCTGCTTATATCAGTATACTTCATAGCAAATTTCGTCGTGCCAGATTTTATAAGCAAGTATTTTGGATTTGATAAACTAGAGGGAGACCAGAAGGTTGATGATGCAGAAGATAAATAG

Protein sequence

>MS.gene065537.t1

MTCLHLPSSLLLMHTKLKPQQQHYLRMEHNTKLLWGEPSKTMPHSKHFSSVSSLRTQAIPDIIVVAATSLPQPADPSTFLRVSALLISVYFIANFVVPDFISKYFGFDKLEGDQKVDDAEDK